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  1 / 1122 MEDLINE  
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[PMID]:29324755
[Au] Autor:Saracino A; Cozzi-Lepri A; Shanyinde M; Ceccherini Silberstein F; Nozza S; Di Biagio A; Cassola G; Bruno G; Capobianchi M; Puoti M; Monno L; d'Arminio Monforte A; ICONA Foundation Study
[Ad] Endereço:Clinic of Infectious Diseases, University of Bari, Bari, Italy.
[Ti] Título:HIV-1 co-receptor tropism and liver fibrosis in HIV-infected patients.
[So] Source:PLoS One;13(1):e0190302, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In vitro, gp120 of both X4 and R5 HIV-1 strains activates human hepatic stellate cells, but if it can promote liver fibrosis in vivo is unknown. We aimed to evaluate if patients carrying X4 or R5 strains have a different liver fibrosis (LF) progression over time. METHODS: A total of 1,137 HIV-infected patients in ICONA cohort (21% females, 7% HCV co-infected) with an available determination of HIV-1 co-receptor tropism (CRT), a Fibrosis-4 Index for Liver Fibrosis (FIB-4) <3.25 and at least one-year follow-up were included. CRT was assessed by gp120 sequencing on plasma RNA and geno2pheno algorithm (10% false positive rate) or by Trofile. LF was assessed by means of FIB-4. LF progression was defined as an absolute score increase or a transition to higher fibrosis stratum and/or occurrence of liver-related clinical events. RESULTS: A total of 249 (22%) patients carried X4 strains, which were associated with older age, lower CD4 count, lower nadir CD4, and intravenous drug use. Overall, X4 and R5 patients had similar baseline FIB-4 scores and similar mean FIB-4 slope after a median follow-up of 35 months. There was no difference between X4 and R5 for time to LF progression (p = 0.925). Estimated risk of LF at 24 months (95% CI) after baseline in X4 and R5 was 10.6% (8.3-12.9) and 9.9% (5.9-14.0), respectively. Age, HCV co-infection, diabetes, HIV-duration, HIV-RNA>100.000 cp/mL, antiretroviral therapy exposure were associated with LF progression at multivariate analysis. CONCLUSIONS: A slight LF progression over time was observed in HIV-infected patients. No difference was demonstrated for X4 and R5 HIV-1 strains in accelerating LF evolution.
[Mh] Termos MeSH primário: Infecções por HIV/complicações
HIV-1/fisiologia
Cirrose Hepática/complicações
Tropismo Viral/fisiologia
[Mh] Termos MeSH secundário: Fármacos Anti-HIV/uso terapêutico
Feminino
Infecções por HIV/tratamento farmacológico
Seres Humanos
Masculino
Receptores Virais/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-HIV Agents); 0 (Receptors, Virus)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190302


  2 / 1122 MEDLINE  
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[PMID]:28463988
[Au] Autor:Calton CM; Bronnimann MP; Manson AR; Li S; Chapman JA; Suarez-Berumen M; Williamson TR; Molugu SK; Bernal RA; Campos SK
[Ad] Endereço:BIO5 Institute, University of Arizona, Tucson, Arizona, United States of America.
[Ti] Título:Translocation of the papillomavirus L2/vDNA complex across the limiting membrane requires the onset of mitosis.
[So] Source:PLoS Pathog;13(5):e1006200, 2017 May.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The human papillomavirus type 16 (HPV16) L2 protein acts as a chaperone to ensure that the viral genome (vDNA) traffics from endosomes to the trans-Golgi network (TGN) and eventually the nucleus, where HPV replication occurs. En route to the nucleus, the L2/vDNA complex must translocate across limiting intracellular membranes. The details of this critical process remain poorly characterized. We have developed a system based on subcellular compartmentalization of the enzyme BirA and its cognate substrate to detect membrane translocation of L2-BirA from incoming virions. We find that L2 translocation requires transport to the TGN and is strictly dependent on entry into mitosis, coinciding with mitotic entry in synchronized cells. Cell cycle arrest causes retention of L2/vDNA at the TGN; only release and progression past G2/M enables translocation across the limiting membrane and subsequent infection. Microscopy of EdU-labeled vDNA reveals a rapid and dramatic shift in vDNA localization during early mitosis. At late G2/early prophase vDNA egresses from the TGN to a pericentriolar location, accumulating there through prometaphase where it begins to associate with condensed chromosomes. By metaphase and throughout anaphase the vDNA is seen bound to the mitotic chromosomes, ensuring distribution into both daughter nuclei. Mutations in a newly defined chromatin binding region of L2 potently blocked translocation, suggesting that translocation is dependent on chromatin binding during prometaphase. This represents the first time a virus has been shown to functionally couple the penetration of limiting membranes to cellular mitosis, explaining in part the tropism of HPV for mitotic basal keratinocytes.
[Mh] Termos MeSH primário: Proteínas do Capsídeo/metabolismo
Genoma Viral/genética
Papillomavirus Humano 16/fisiologia
Mitose
Proteínas Oncogênicas Virais/metabolismo
Infecções por Papillomavirus/virologia
[Mh] Termos MeSH secundário: Transporte Biológico
Proteínas do Capsídeo/genética
Pontos de Checagem do Ciclo Celular
Linhagem Celular
Núcleo Celular/metabolismo
Núcleo Celular/virologia
DNA Viral/genética
DNA Viral/metabolismo
Endossomos/metabolismo
Endossomos/virologia
Papillomavirus Humano 16/genética
Seres Humanos
Queratinócitos/virologia
Mutação
Proteínas Oncogênicas Virais/genética
Tropismo Viral
Vírion
Internalização do Vírus
Rede trans-Golgi/metabolismo
Rede trans-Golgi/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (DNA, Viral); 0 (L2 protein, Human papillomavirus type 16); 0 (Oncogene Proteins, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006200


  3 / 1122 MEDLINE  
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[PMID]:29216317
[Au] Autor:Fraser JE; De Bruyne JT; Iturbe-Ormaetxe I; Stepnell J; Burns RL; Flores HA; O'Neill SL
[Ad] Endereço:Institute of Vector-Borne Disease, Monash University, Clayton, VIC, Australia.
[Ti] Título:Novel Wolbachia-transinfected Aedes aegypti mosquitoes possess diverse fitness and vector competence phenotypes.
[So] Source:PLoS Pathog;13(12):e1006751, 2017 Dec.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wolbachia pipientis from Drosophila melanogaster (wMel) is an endosymbiotic bacterium that restricts transmission of human pathogenic flaviviruses and alphaviruses, including dengue, Zika, and chikungunya viruses, when introduced into the mosquito vector Aedes aegypti. To date, wMel-infected Ae. aegypti have been released in field trials in 5 countries to evaluate the effectiveness of this strategy for disease control. Despite the success in establishing wMel-infected mosquitoes in wild populations, and the well-characterized antiviral capabilities of wMel, transinfecting different or additional Wolbachia strains into Ae. aegypti may improve disease impact, and perhaps more importantly, could provide a strategy to account for the possible evolution of resistant arboviruses. Here, we report the successful transinfection of Ae. aegypti with the Wolbachia strains wMelCS (D. melanogaster), wRi (D. simulans) and wPip (Culex quinquefasciatus) and assess the effects on Ae. aegypti fitness, cytoplasmic incompatibility, tissue tropism and pathogen blocking in a laboratory setting. The results demonstrate that wMelCS provides a similar degree of protection against dengue virus as wMel following an infectious blood meal, and significantly reduces viral RNA levels beyond that of wMel following a direct challenge with infectious virus in mosquitoes, with no additional fitness cost to the host. The protection provided by wRi is markedly weaker than that of wMelCS, consistent with previous characterisations of these lines in Drosophila, while wPip was found to substantially reduce the fitness of Ae. aegypti. Thus, we determine wMelCS as a key candidate for further testing in field-relevant fitness tests and viremic blood feeding challenges in a clinical setting to determine if it may represent an alternative Wolbachia strain with more desirable attributes than wMel for future field testing.
[Mh] Termos MeSH primário: Aedes/microbiologia
Transmissão Vertical de Doença Infecciosa/veterinária
Mosquitos Vetores/microbiologia
Wolbachia/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Aedes/crescimento & desenvolvimento
Aedes/fisiologia
Aedes/virologia
Animais
Controle de Doenças Transmissíveis/métodos
Culex/microbiologia
Vírus da Dengue/isolamento & purificação
Vírus da Dengue/fisiologia
Drosophila melanogaster/microbiologia
Drosophila simulans/microbiologia
Feminino
Fertilidade
Masculino
Controle de Mosquitos/métodos
Mosquitos Vetores/fisiologia
Mosquitos Vetores/virologia
Especificidade de Órgãos
Ovário/microbiologia
Ovário/fisiologia
RNA Viral/isolamento & purificação
Glândulas Salivares/microbiologia
Glândulas Salivares/fisiologia
Caracteres Sexuais
Especificidade da Espécie
Análise de Sobrevida
Tropismo Viral
Wolbachia/isolamento & purificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006751


  4 / 1122 MEDLINE  
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[PMID]:29244846
[Au] Autor:Alvarez-Quinto RA; Cornejo-Franco JF; Quito-Avila DF
[Ad] Endereço:Centro de Investigaciones Biotecnológicas del Ecuador, CIBE, Escuela Superior Politécnica del Litoral, ESPOL, Guayaquil, Ecuador.
[Ti] Título:Characterization of a not so new potexvirus from babaco (Vasconcellea x heilbornii).
[So] Source:PLoS One;12(12):e0189519, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new member of the genus Potexvirus was fully sequenced and characterized. The virus was isolated from babaco (Vasconcellea x heilbornii), a natural hybrid native to Ecuador. The virus contains a 6,692 nt long genome organized in five open reading frames in an arrangement typical of other potexviruses. Sequence comparisons revealed close relatedness with Papaya mosaic virus (PapMV), Alternathera mosaic virus (AltMV) and Senna mosaic virus (SenMV), exhibiting nucleotide identities up to 67% for the polymerase (Pol) and 68% for the coat protein (CP), with deduced amino acid identities of 70% and 72% for the Pol and CP, respectively. The presence of an AlkB domain, in the polymerase region, was observed. Terminal nucleotide sequences were conserved across potexviruses with characteristic motifs and predicted secondary structures at the 3' UTR. Although serologically undistinguishable from PapMV and AltMV, the new virus showed differences in host range and symptom induction. The name babaco mosaic virus is proposed for this newly characterized Potexvirus. The complete genome sequence of the new virus has been deposited in NCBI GenBank under accession number MF978248.
[Mh] Termos MeSH primário: Magnoliopsida/virologia
Potexvirus/genética
[Mh] Termos MeSH secundário: Genes Virais
Especificidade de Hospedeiro
Filogenia
Potexvirus/isolamento & purificação
Análise de Sequência de DNA
Proteínas Virais/genética
Tropismo Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189519


  5 / 1122 MEDLINE  
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[PMID]:29260200
[Au] Autor:Reid CA; Ertel KJ; Lipinski DM
[Ad] Endereço:Department of Ophthalmology, Eye Institute, Medical College of Wisconsin, Milwaukee, Wisconsin, United States.
[Ti] Título:Improvement of Photoreceptor Targeting via Intravitreal Delivery in Mouse and Human Retina Using Combinatory rAAV2 Capsid Mutant Vectors.
[So] Source:Invest Ophthalmol Vis Sci;58(14):6429-6439, 2017 Dec 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: Effective intravitreal gene delivery to cells of the central retina (i.e., photoreceptors) would be of substantial benefit for treating patients with retinal diseases, such as achromatopsia, where retinal detachment from a subretinal may be harmful. Previous studies demonstrated that mutation of the recombinant adeno-associated virus (rAAV) capsid through introduction of peptide insertions or amino acid substitutions dramatically alters vector tropism. Herein, we evaluate the photoreceptor transduction efficiency of three rAAV2/2-based capsid mutant vectors: rAAV2/2[7m8], rAAV2/2[QuadYF+TV], and a chimeric vector incorporating both mutations (termed rAAV2/2[MAX]) following intravitreal delivery in mice. Furthermore, we evaluate the transduction efficiency of rAAV2/2[MAX] using explanted human central retinal samples to address clinical translatability. Methods: Vectors containing a GFP or mCherry reporter gene were intravitreally injected into C57BL/6J or Nrl-EGFP mice, respectively. Transduction was assessed in vivo utilizing a custom multiline confocal scanning laser ophthalmoscope. Injected Nrl-EGFP mouse retinas were used to quantify transduced photoreceptors using flow cytometry. Postmortem human retinal tissue was cultured following administration of rAAV2/2[MAX]. C57BL/6J retinas and human explants were cryosectioned to determine vector tropism. Results: The chimeric vector rAAV2/2[MAX] transduced significantly higher proportions of the retina than did either single mutant serotypes following intravitreal delivery in murine retina, including inner retinal cells and photoreceptors. Vector rAAV2[MAX] demonstrated transduction of human photoreceptors and ganglion cells. Conclusions: Transduction observed via rAAV2/2[MAX] indicates that combining mutations with complementary mechanisms of action in a single vector results in enhanced transduction. rAAV2/2[MAX] also presented the ability to transduce human photoreceptors and ganglion cells, indicating potential for efficient intravitreal vector delivery.
[Mh] Termos MeSH primário: Capsídeo/metabolismo
Dependovirus/genética
Técnicas de Transferência de Genes
Terapia Genética/métodos
Vetores Genéticos/administração & dosagem
Células Fotorreceptoras de Vertebrados/metabolismo
Retina/metabolismo
[Mh] Termos MeSH secundário: Animais
Dependovirus/fisiologia
Seres Humanos
Injeções Intravítreas
Camundongos
Camundongos Endogâmicos C57BL
Regiões Promotoras Genéticas/genética
Transdução Genética
Tropismo Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-22281


  6 / 1122 MEDLINE  
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[PMID]:28985234
[Au] Autor:McDonald EM; Duggal NK; Brault AC
[Ad] Endereço:Division of Vector-borne Diseases, National Center for Emerging Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, United States of America.
[Ti] Título:Pathogenesis and sexual transmission of Spondweni and Zika viruses.
[So] Source:PLoS Negl Trop Dis;11(10):e0005990, 2017 Oct.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Spondweni serogroup of viruses (Flaviviridae, Flavivirus) is comprised of Spondweni virus (SPONV) and Zika virus (ZIKV), which are mosquito-borne viruses capable of eliciting human disease. Numerous cases of ZIKV sexual transmission in humans have been documented following the emergence of the Asian genotype in the Americas. The African ZIKV genotype virus was previously implicated in the first reported case of ZIKV sexual transmission. Reports of SPONV infection in humans have been associated with non-specific febrile illness, but no association with sexual transmission has been reported. In order to assess the relative efficiency of sexual transmission of different ZIKV strains and the potential capacity of SPONV to be sexually transmitted, viral loads in the male reproductive tract and in seminal fluids were assessed in interferon α/ß and -γ receptor deficient (AG129) mice. Male mice were inoculated subcutaneously with Asian genotype ZIKV strains PRVABC59 (Puerto Rico, 2015), FSS13025 (Cambodia, 2010), or P6-740 (Malaysia, 1966); African genotype ZIKV strain DakAr41524 (Senegal, 1984); or SPONV strain SAAr94 (South Africa, 1955). Infectious virus was detected in 60-72% of ejaculates collected from AG129 mice inoculated with ZIKV strains. In contrast, only 4% of ejaculates from SPONV-inoculated AG129 males were found to contain infectious virus, despite viral titers in the testes that were comparable to those of ZIKV-inoculated mice. Based on these results, future studies should be undertaken to assess the role of viral genetic determinants and host tropism that dictate the differential sexual transmission potential of ZIKV and SPONV.
[Mh] Termos MeSH primário: Sêmen/virologia
Doenças Virais Sexualmente Transmissíveis/transmissão
Carga Viral
Tropismo Viral/fisiologia
Infecção pelo Zika virus/transmissão
Zika virus/classificação
[Mh] Termos MeSH secundário: Aedes/virologia
Animais
Linhagem Celular
Cercopithecus aethiops
Modelos Animais de Doenças
Seres Humanos
Masculino
Camundongos
Mosquitos Vetores/virologia
Doenças Virais Sexualmente Transmissíveis/patologia
Doenças Virais Sexualmente Transmissíveis/virologia
Células Vero
Replicação Viral
Infecção pelo Zika virus/patologia
Infecção pelo Zika virus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005990


  7 / 1122 MEDLINE  
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[PMID]:28945790
[Au] Autor:Del Prete GQ; Keele BF; Fode J; Thummar K; Swanstrom AE; Rodriguez A; Raymond A; Estes JD; LaBranche CC; Montefiori DC; KewalRamani VN; Lifson JD; Bieniasz PD; Hatziioannou T
[Ad] Endereço:AIDS and Cancer Virus Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, United States of America.
[Ti] Título:A single gp120 residue can affect HIV-1 tropism in macaques.
[So] Source:PLoS Pathog;13(9):e1006572, 2017 Sep.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Species-dependent variation in proteins that aid or limit virus replication determines the ability of lentiviruses to jump between host species. Identifying and overcoming these differences facilitates the development of animal models for HIV-1, including models based on chimeric SIVs that express HIV-1 envelope (Env) glycoproteins, (SHIVs) and simian-tropic HIV-1 (stHIV) strains. Here, we demonstrate that the inherently poor ability of most HIV-1 Env proteins to use macaque CD4 as a receptor is improved during adaptation by virus passage in macaques. We identify a single amino acid, A281, in HIV-1 Env that consistently changes during adaptation in macaques and affects the ability of HIV-1 Env to use macaque CD4. Importantly, mutations at A281 do not markedly affect HIV-1 Env neutralization properties. Our findings should facilitate the design of HIV-1 Env proteins for use in non-human primate models and thus expedite the development of clinically relevant reagents for testing interventions against HIV-1.
[Mh] Termos MeSH primário: Proteína gp120 do Envelope de HIV/química
Infecções por HIV/virologia
HIV-1/fisiologia
Tropismo Viral/fisiologia
[Mh] Termos MeSH secundário: Adaptação Fisiológica/fisiologia
Animais
Antígenos CD4/metabolismo
Modelos Animais de Doenças
Feminino
Citometria de Fluxo
Proteína gp120 do Envelope de HIV/genética
Infecções por HIV/genética
Seres Humanos
Immunoblotting
Macaca mulatta
Masculino
Reação em Cadeia da Polimerase
Vírus da Imunodeficiência Símia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD4 Antigens); 0 (HIV Envelope Protein gp120); 0 (gp120 protein, Human immunodeficiency virus 1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006572


  8 / 1122 MEDLINE  
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[PMID]:28934452
[Au] Autor:Belser JA; Creager HM; Zeng H; Maines TR; Tumpey TM
[Ad] Endereço:Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention.
[Ti] Título:Pathogenesis, Transmissibility, and Tropism of a Highly Pathogenic Avian Influenza A(H7N7) Virus Associated With Human Conjunctivitis in Italy, 2013.
[So] Source:J Infect Dis;216(suppl_4):S508-S511, 2017 Sep 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:H7 subtype influenza viruses represent a persistent public health threat because of their continued detection in poultry and ability to cause human infection. An outbreak of highly pathogenic avian influenza H7N7 virus in Italy during 2013 resulted in 3 cases of human conjunctivitis. We determined the pathogenicity and transmissibility of influenza A/Italy/3/2013 virus in mouse and ferret models and examined the replication kinetics of this virus in several human epithelial cell types. The moderate virulence observed in mammalian models and the capacity for transmission in a direct contact model underscore the need for continued study of H7 subtype viruses.
[Mh] Termos MeSH primário: Conjuntivite Viral/diagnóstico
Vírus da Influenza A Subtipo H7N7/isolamento & purificação
Influenza Aviária/diagnóstico
Influenza Aviária/transmissão
Influenza Humana/diagnóstico
Tropismo Viral
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Modelos Animais de Doenças
Epitélio Posterior/citologia
Epitélio Posterior/virologia
Feminino
Furões/virologia
Seres Humanos
Vírus da Influenza A Subtipo H7N7/fisiologia
Itália/epidemiologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Mucosa Nasal/citologia
Mucosa Nasal/virologia
Aves Domésticas/virologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jiw559


  9 / 1122 MEDLINE  
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Montassier, Hélio José
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[PMID]:28895517
[Au] Autor:Santos Fernando F; Coelho Kasmanas T; Diniz Lopes P; da Silva Montassier MF; Zanella Mores MA; Casagrande Mariguela V; Pavani C; Moreira Dos Santos R; Assayag MS; Montassier HJ
[Ad] Endereço:1​Department of Veterinary Pathology, Laboratory of Virology and Immunology, Universidade Estadual Paulista Júlio de Mesquita Filho (FCAV- UNESP), Jaboticabal, SP 14884-900, Brazil.
[Ti] Título:Assessment of molecular and genetic evolution, antigenicity and virulence properties during the persistence of the infectious bronchitis virus in broiler breeders.
[So] Source:J Gen Virol;98(10):2470-2481, 2017 Oct.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The infectious bronchitis virus (IBV) causes a highly contagious disease [infectious bronchitis (IB)] that results in substantial economic losses to the poultry industry worldwide. We conducted a molecular and phylogenetic analysis of the S1 gene of Brazilian (BR) IBV isolates from a routinely vaccinated commercial flock of broiler breeders, obtained from clinical IB episodes that occurred in 24-, 46- and 62-week-old chickens. We also characterized the antigenicity, pathogenesis, tissue tropism and spreading of three IBV isolates by experimental infection of specific pathogen-free (SPF) chickens and contact sentinel birds. The results reveal that the three IBV isolates mainly exhibited mutations in the hypervariable regions (HVRs) of the S1 gene and protein, but were phylogenetically and serologically closely related, belonging to lineage 11 of the GI genotype, the former BR genotype I. All three isolates caused persistent infection in broiler breeders reared in the field, despite high systemic anti-IBV antibody titres, and exhibited tropism and pathogenicity for the trachea and kidney after experimental infection in SPF chickens and contact birds. In conclusion, BR genotype I isolates of IBV evolve continuously during the productive cycle of persistently infected broiler breeders, causing outbreaks that are not impaired by the current vaccination programme with Massachusetts vaccine strains. In addition, the genetic alterations in the S1 gene of these isolates were not able to change their tissue tropism and pathogenicity, but did seem to negatively influence the effectiveness of the host immune responses against these viruses, and favour viral persistence.
[Mh] Termos MeSH primário: Proteínas do Capsídeo/genética
Galinhas/virologia
Infecções por Coronavirus/veterinária
Vírus da Bronquite Infecciosa/genética
Vírus da Bronquite Infecciosa/imunologia
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Brasil
Infecções por Coronavirus/imunologia
Infecções por Coronavirus/virologia
Vírus da Bronquite Infecciosa/isolamento & purificação
Vírus da Bronquite Infecciosa/patogenicidade
Rim/virologia
Traqueia/virologia
Tropismo Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000893


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[PMID]:28888112
[Au] Autor:Harper SJ; Cowell SJ; Dawson WO
[Ad] Endereço:Department of Plant Pathology, Washington State University, Prosser, WA 99350, USA. Electronic address: scott.harper@wsu.edu.
[Ti] Título:Isolate fitness and tissue-tropism determine superinfection success.
[So] Source:Virology;511:222-228, 2017 Nov.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mechanism of cross-protection, the deliberate infection of plants with a "mild" virus isolate to protect against "severe" isolates, has long been a topic of debate. In our model system, Citrus tristeza virus (CTV), this appears to be genotype-specific superinfection-exclusion, suggesting a simple recipe for cross-protection. However, this concept failed in field trials, which led us to examine the process of superinfection-exclusion more closely. We found that exclusion relies on the relative fitness of the primary versus the challenge isolates, and the host infected, and that significant differences in superinfection success could occur between isolates that differ by as few as 3 nucleotides. Furthermore, we found that exclusion was not uniform throughout the plant, but was tissue-specific. These data suggest that cross-protection is not a simple like-for-like process but a complex interaction between the primary and challenge isolates and the host.
[Mh] Termos MeSH primário: Citrus/virologia
Closterovirus/fisiologia
Doenças das Plantas/virologia
Superinfecção/virologia
Interferência Viral
Tropismo Viral
[Mh] Termos MeSH secundário: Interações Hospedeiro-Patógeno
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170910
[St] Status:MEDLINE



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