Base de dados : MEDLINE
Pesquisa : G06.550.050 [Categoria DeCS]
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[PMID]:28461449
[Au] Autor:Stubbendieck RM; Straight PD
[Ad] Endereço:Interdisciplinary Program in Genetics, Texas A&M University, College Station, Texas, USA.
[Ti] Título:Linearmycins Activate a Two-Component Signaling System Involved in Bacterial Competition and Biofilm Morphology.
[So] Source:J Bacteriol;199(18), 2017 09 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteria use two-component signaling systems to adapt and respond to their competitors and changing environments. For instance, competitor bacteria may produce antibiotics and other bioactive metabolites and sequester nutrients. To survive, some species of bacteria escape competition through antibiotic production, biofilm formation, or motility. Specialized metabolite production and biofilm formation are relatively well understood for bacterial species in isolation. How bacteria control these functions when competitors are present is not well studied. To address fundamental questions relating to the competitive mechanisms of different species, we have developed a model system using two species of soil bacteria, and sp. strain Mg1. Using this model, we previously found that linearmycins produced by sp. strain Mg1 cause lysis of cells and degradation of colony matrix. We identified strains of with mutations in the two-component signaling system operon that confer dual phenotypes of specific linearmycin resistance and biofilm morphology. We determined that expression of the ATP-binding cassette (ABC) transporter operon, particularly and , is necessary for biofilm morphology. Using transposon mutagenesis, we identified genes that are required for YfiLMN-mediated biofilm morphology, including several chaperones. Using transcriptional fusions, we found that YfiJ signaling is activated by linearmycins and other polyene metabolites. Finally, using a truncated YfiJ, we show that YfiJ requires its transmembrane domain to activate downstream signaling. Taken together, these results suggest coordinated dual antibiotic resistance and biofilm morphology by a single multifunctional ABC transporter promotes competitive fitness of DNA sequencing approaches have revealed hitherto unexplored diversity of bacterial species in a wide variety of environments that includes the gastrointestinal tract of animals and the rhizosphere of plants. Interactions between different species in bacterial communities have impacts on our health and industry. However, many approaches currently used to study whole bacterial communities do not resolve mechanistic details of interspecies interactions, including how bacteria sense and respond to their competitors. Using a competition model, we have uncovered dual functions for a previously uncharacterized two-component signaling system involved in specific antibiotic resistance and biofilm morphology. Insights gleaned from signaling within interspecies interaction models build a more complete understanding of gene functions important for bacterial communities and will enhance community-level analytical approaches.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Antibiose
Bacillus subtilis/fisiologia
Biofilmes/crescimento & desenvolvimento
Viabilidade Microbiana
Streptomyces/fisiologia
[Mh] Termos MeSH secundário: Transportadores de Cassetes de Ligação de ATP/metabolismo
Fusão Gênica Artificial
Bacillus subtilis/efeitos dos fármacos
Elementos de DNA Transponíveis
Perfilação da Expressão Gênica
Regulação Bacteriana da Expressão Gênica
Mutagênese Insercional
Mutação
Transdução de Sinais
Streptomyces/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (ATP-Binding Cassette Transporters); 0 (Anti-Bacterial Agents); 0 (DNA Transposable Elements)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:27773522
[Au] Autor:Tamulaitis G; Venclovas C; Siksnys V
[Ad] Endereço:Institute of Biotechnology, Vilnius University, Sauletekio av. 7, Vilnius 10257, Lithuania.
[Ti] Título:Type III CRISPR-Cas Immunity: Major Differences Brushed Aside.
[So] Source:Trends Microbiol;25(1):49-61, 2017 Jan.
[Is] ISSN:1878-4380
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:For a long time the mechanism of immunity provided by the Type III CRISPR-Cas systems appeared to be inconsistent: the Type III-A Csm complex of Staphylococcus epidermidis was first reported to target DNA while Type III-B Cmr complexes were shown to target RNA. This long-standing conundrum has now been resolved by finding that the Type III CRISPR-Cas systems are both RNases and target RNA-activated DNA nucleases. The immunity is achieved by coupling binding and cleavage of RNA transcripts to the degradation of invading DNA. The base-pairing potential between the target RNA and the CRISPR RNA (crRNA) 5'-handle seems to play an important role in discriminating self and non-self nucleic acids; however, the detailed mechanism remains to be uncovered.
[Mh] Termos MeSH primário: Antibiose/genética
Sistemas CRISPR-Cas/genética
Proteínas de Ligação a DNA/genética
DNA/metabolismo
Proteínas de Ligação a RNA/genética
RNA/metabolismo
Staphylococcus epidermidis/genética
[Mh] Termos MeSH secundário: Sistemas CRISPR-Cas/fisiologia
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (RNA-Binding Proteins); 63231-63-0 (RNA); 9007-49-2 (DNA)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:29251479
[Au] Autor:Thiyagarajan S; Bavya M; Jamal A
[Ti] Título:Isolation of marine fungi Aspergillus sp. and its in vitro antifouling activity against marine bacteria.
[So] Source:J Environ Biol;37(5):895-903, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Biofouling is considered as a main issue of concern in aquatic environment causing severe economic loss and pollution. The aim of the present study was to isolate marine fungus antagonistic to biofouling bacteria and to define antifouling compounds present in it. Using standard plate method five predominant biofouling bacteria viz., Methylococcus sp., Flavobacterium sp., Marinococcus sp., Serratia sp. and Pseudomonas sp. were isolated from marine solid substances on Zobell's agar. Tolerance range of these bacteria to NaCl was 2-10%. Isolation of fungi from mangrove and estuarine sediments and their screening identified Aspergillus sp. EF4 as a potential isolate. This isolate caused inhibition of all the five test bacterial cultures measuring zone diameters respectively of 11, 16, 12, 13 and 11mm.? Subsequent to submerged fermentation using shaking flask method this fungus produced bioactive compounds within 5 days. The culture parameters optimized were raffinose as carbon source, yeast extract as lone nitrogen source, pH up to 9.0 and temperature up to 40?C. Antifouling compounds of culture filtrate were separated and detected by a three-step procedure involving thin layer chromatography, bioautography and preparative TLC. The in vitro assay involving glass slide-wooden stick-biofilm method revealed that these compounds could cause inhibition and destruction of bacteria to an extent of 2.16 x 104 CFU ml-1 and 2.46 x 104 CFU ml-1 respectively while growth of bacteria in control beaker was enumerated to be 4.41 x 104 CFU ml-1. High performance liquid chromatography of culture filtrate indicated probable principal antifouling compound as Fumonisin B2. Isolation of antagonistic marine fungus from Indian coast and detection of its antifouling compound would help in planning effective strategies for controlling biofouling in marine environment.
[Mh] Termos MeSH primário: Antibiose
Aspergillus/fisiologia
Bactérias/classificação
Incrustação Biológica
[Mh] Termos MeSH secundário: Organismos Aquáticos
Fenômenos Fisiológicos Bacterianos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


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[PMID]:29251469
[Au] Autor:Kaur R; Joshi N; Virk JS; Sharma S
[Ti] Título:Evaluation of Pseudomonas fluorescens for the management of tomato early blight disease and fruit borer.
[So] Source:J Environ Biol;37(5):869-872, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Early blight disease and fruit damage by Helicoverpa armigera are serious problems of tomato causing heavy losses in the yield. An experiment was conducted to evaluate the efficacy of talc based formulation of antagonist Pseudomonas flourescens (Psf) delivered through two different forms of substrate, farmyard manure (FYM) and vermicompost, for the management of tomato early blight disease. Two treatments of Psf, were selected Solarization of nursery soil + seeds and seedlings root dip treatment with Psf formulation + Use of FYM colonized with Psf formulation; Solarization of nursery soil + seed and seedlings root dip treatment with Psf formulation + Use of vermicompost colonized with Psf formulation. These were compared with farmer's practice and untreated control. The impact of all these treatments on tomato fruit borer, H. armigera was also studied. There was not much variation in plant growth parameters (plant height and canopy width) between all the treatments. Both biocontrol treatments (17.69% and 141.3 q acre-1; 15.70% and 139.4 q acre-1, respectively), as well as farmer's practice (10.25% and 208.5 q acre-1) were found to be better than untreated control (29.21% and 94.6 q acre-1) in reducing the incidence of early blight disease in tomato and increasing the marketable yield of tomato fruits. However, the farmer's practice was found to be significantly better than both biocontrol treatments of Psf in controlling fruit damage due to disease, as well as fruit borer. The effect of both bioagents enriched formulations i.e. FYM+Psf and vermicompost+Psf with respect to disease was significantly at par with each other, whereas they had no impact on the fruit borer incidence. The results indicated that either of biocontrol treatments of P. flourescens using farmyard manure and vermicompost, as delivery substrates, can be considered as one of the component along with chemical control in developing IPM programme for the management of early blight disease in tomato.
[Mh] Termos MeSH primário: Antibiose
Lycopersicon esculentum/microbiologia
Mariposas/microbiologia
Controle Biológico de Vetores
Doenças das Plantas/microbiologia
Pseudomonas fluorescens/fisiologia
[Mh] Termos MeSH secundário: Animais
Compostagem
Esterco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Manure)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


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[PMID]:29326272
[Au] Autor:Zhao W; Caro F; Robins W; Mekalanos JJ
[Ti] Título:Antagonism toward the intestinal microbiota and its effect on virulence.
[So] Source:Science;359(6372):210-213, 2018 01 12.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The bacterial type VI secretion system (T6SS) is a nanomachine that delivers toxic effector proteins into target cells, killing them. In mice, we found that the T6SS attacks members of the host commensal microbiota in vivo, facilitating the pathogen's colonization of the gut. This microbial antagonistic interaction drives measurable changes in the pathogenicity of through enhanced intestinal colonization, expression of bacterial virulence genes, and activation of host innate immune genes. Because ablation of mouse commensals by this enteric pathogen correlated with more severe diarrheal symptoms, we conclude that antagonism toward the gut microbiota could improve the fitness of as a pathogen by elevating its transmission to new susceptible hosts.
[Mh] Termos MeSH primário: Antibiose
Cólera/microbiologia
Microbioma Gastrointestinal/fisiologia
Sistemas de Secreção Tipo VI/metabolismo
Vibrio cholerae/fisiologia
Vibrio cholerae/patogenicidade
[Mh] Termos MeSH secundário: Animais
Cólera/imunologia
Cólera/metabolismo
Citocinas/genética
Citocinas/metabolismo
Escherichia coli/crescimento & desenvolvimento
Regulação da Expressão Gênica
Regulação Bacteriana da Expressão Gênica
Aptidão Genética
Intestinos/imunologia
Intestinos/metabolismo
Intestinos/microbiologia
Camundongos
Mutação
Simbiose
Transcrição Genética
Sistemas de Secreção Tipo VI/genética
Vibrio cholerae/genética
Vibrio cholerae/crescimento & desenvolvimento
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Cytokines); 0 (Type VI Secretion Systems)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1126/science.aap8775


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[PMID]:29293082
[Au] Autor:Hernandez Reyes Y; Provost C; Traesel CK; Jacques M; Gagnon CA
[Ad] Endereço:Centre de recherche en infectiologie porcine et avicole (CRIPA) et Groupe de recherche sur les maladies infectieuses en production animale (GREMIP), Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, Québec, J2S 2M2, Canada.
[Ti] Título:Actinobacillus pleuropneumoniae culture supernatant antiviral effect against porcine reproductive and respiratory syndrome virus occurs prior to the viral genome replication and transcription through actin depolymerization.
[So] Source:J Med Microbiol;67(2):249-264, 2018 Feb.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Recently, the strong antiviral activity of an Actinobacillus pleuropneumoniae (App) culture supernatant against porcine reproductive and respiratory syndrome virus (PRRSV) was discovered. Following this finding, the objective of the present study was to understand how the App culture supernatant inhibits PRRSV replication in its natural targeted host cells, i.e. porcine alveolar macrophages (PAMs). METHODOLOGY: Several assays were conducted with App culture supernatant-treated PRRSV-infected cell lines, such as PAM, St-Jude porcine lung and MARC-145 cells. RT-qPCR assays were used to determine the expression levels of type I and II IFN mRNAs, viral genomic (gRNA) and sub-genomic RNAs (sgRNAs). Proteomic, Western blot and immunofluorescence assays were conducted to determine the involvement of actin filaments in the App culture supernatant antiviral effect.Results/Key findings. Type I and II IFN mRNA expressions were not upregulated by the App culture supernatant. Time courses of gRNA and sgRNA expression levels demonstrated that the App culture supernatant inhibits PRRSV infection before the first viral transcription cycle. Western blot experiments confirmed an increase in the expression of cofilin (actin cytoskeleton dynamics regulator) and immunofluorescence also demonstrated a significant decrease of actin filaments in App culture supernatant-treated PRRSV-infected PAM cells. App culture supernatant antiviral activity was also demonstrated against other PRRSV strains of genotypes I and II. CONCLUSION: App culture supernatant antiviral effect against PRRSV takes place early during PRRSV infection. Results suggest that App culture supernatant antiviral effect may take place via the activation of cofilin, which induces actin depolymerization and subsequently, probably affects PRRSV endocytosis. Other experiments are needed to fully validate this latest hypothesis.
[Mh] Termos MeSH primário: Actinobacillus pleuropneumoniae/fisiologia
Actinas/metabolismo
Antivirais/farmacologia
Vírus da Síndrome Respiratória e Reprodutiva Suína/fisiologia
Replicação Viral/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antibiose
Linhagem Celular
Meios de Cultura/química
Genoma Viral
Interferon Tipo I/genética
Macrófagos Alveolares/efeitos dos fármacos
Macrófagos Alveolares/virologia
Vírus da Síndrome Respiratória e Reprodutiva Suína/genética
Reação em Cadeia da Polimerase em Tempo Real
Suínos
Transcrição Genética/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Antiviral Agents); 0 (Culture Media); 0 (Interferon Type I)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000659


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[PMID]:29320571
[Au] Autor:Gao P; Qin J; Li D; Zhou S
[Ad] Endereço:College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.
[Ti] Título:Inhibitory effect and possible mechanism of a Pseudomonas strain QBA5 against gray mold on tomato leaves and fruits caused by Botrytis cinerea.
[So] Source:PLoS One;13(1):e0190932, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The fungal pathogen Botrytis cinerea causes gray mold disease on various hosts, which results in serious economic losses. Over the past several decades, many kinds of fungicides have been used to successfully control the disease. Meanwhile, the uses of fungicides lead to environmental pollution as well as a potential threat to the human health by the chemical residues in tomato fruit. Also, the gray mold disease is difficult to control with fungicides. Therefore, exploring alternative measures such as biological controls could be the best choice to control the disease and alleviate damages caused by fungicides. In this study, we isolated and identified a novel Pseudomonas strain termed as QBA5 from healthy tomato plant based on the morphological, biochemical characteristics and molecular detection. The antifungal activity assays revealed that, in the presence of QBA5, conidia germination, germ tube elongation and mycelial growth of B. cinerea were significantly inhibited. Most importantly, QBA5 exerted a significant preventive effectiveness against gray mold on tomato fruits and plants. The possible mechanism of QBA5 involved in the inhibition of B. cinerea was investigated. It revealed that the conidia plasma membrane of B. cinerea was severely damaged by QBA5. Further, four different antifungal compounds in the supernatant of QBA5 were separated by preparative high performance liquid chromatography (PHPLC). Overall, the data indicate that there is a considerable potential for QBA5 to reduce the damage caused by gray mold disease on tomato.
[Mh] Termos MeSH primário: Antibiose
Botrytis/fisiologia
Frutas/microbiologia
Lycopersicon esculentum/microbiologia
Doenças das Plantas/microbiologia
Folhas de Planta/microbiologia
Pseudomonas/fisiologia
[Mh] Termos MeSH secundário: Antifúngicos/química
Antifúngicos/farmacologia
Botrytis/efeitos dos fármacos
Membrana Celular/efeitos dos fármacos
Membrana Celular/fisiologia
Meios de Cultura/química
Frutas/efeitos dos fármacos
Lycopersicon esculentum/efeitos dos fármacos
Doenças das Plantas/prevenção & controle
Doenças das Plantas/terapia
Folhas de Planta/efeitos dos fármacos
Pseudomonas/isolamento & purificação
Esporos Fúngicos/efeitos dos fármacos
Esporos Fúngicos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Culture Media)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190932


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[PMID]:28470335
[Au] Autor:Han GG; Song AA; Kim EB; Yoon SH; Bok JD; Cho CS; Kil DY; Kang SK; Choi YJ
[Ad] Endereço:Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
[Ti] Título:Improved antimicrobial activity of Pediococcus acidilactici against Salmonella Gallinarum by UV mutagenesis and genome shuffling.
[So] Source:Appl Microbiol Biotechnol;101(13):5353-5363, 2017 Jul.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Pediococcus acidilactici is a widely used probiotic, and Salmonella enterica serovar Gallinarum (SG) is a significant pathogen in the poultry industry. In this study, we improved the antimicrobial activity of P. acidilactici against SG using UV mutation and genome shuffling (GS). To improve antimicrobial activity against SG, UV mutagenesis was performed against wild-type P. acidilactici (WT), and five mutants showed improved antimicrobial activity. To further improve antimicrobial activity, GS was performed on five UV mutants. Following GS, four mutants showed improved antimicrobial activity compared with the UV mutants and WT. The antimicrobial activity of GS1 was highest among the mutants; however, the activity was reduced when the culture supernatant was treated with proteinase K, suggesting that the improved antimicrobial activity is due to a proteinous substance such as bacteriocin. To validate the activity of GS1 in vivo, we designed multi-species probiotics and performed broiler feeding experiments. Groups consisted of no treatment (NC), avilamycin-treated (PC), probiotic group 1 containing WT (T1), and probiotic group 2 containing GS1 (T2). In broiler feeding experiments, coliform bacteria were significantly reduced in T2 compared with NC, PC, and T1. The cecal microbiota was modulated and pathogenic bacteria were reduced by GS1 oral administration. In this study, GS1 showed improved antimicrobial activity against SG in vitro and reduced pathogenic bacteria in a broiler feeding experiment. These results suggest that GS1 can serve as an efficient probiotic, as an alternative to antibiotics in the poultry industry.
[Mh] Termos MeSH primário: Antibiose
Embaralhamento de DNA
Mutagênese
Pediococcus acidilactici/genética
Pediococcus acidilactici/fisiologia
Probióticos
Salmonella/fisiologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Anti-Infecciosos
Bacteriocinas/biossíntese
Bacteriocinas/farmacologia
Ceco/microbiologia
Galinhas/microbiologia
Meios de Cultura/química
Endopeptidase K/metabolismo
Genoma Bacteriano
Pediococcus acidilactici/efeitos dos fármacos
Pediococcus acidilactici/efeitos da radiação
Doenças das Aves Domésticas/microbiologia
Doenças das Aves Domésticas/terapia
Probióticos/química
Salmonella/efeitos dos fármacos
Salmonelose Animal/microbiologia
Salmonelose Animal/terapia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents); 0 (Bacteriocins); 0 (Culture Media); EC 3.4.21.64 (Endopeptidase K)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-017-8293-6


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[PMID]:28859156
[Au] Autor:Barman S; Ghosh R; Sengupta S; Mandal NC
[Ad] Endereço:Mycology and Plant Pathology Laboratory, Department of Botany, Visva-Bharati, Santiniketan, West Bengal, India.
[Ti] Título:Longterm storage of post-packaged bread by controlling spoilage pathogens using Lactobacillus fermentum C14 isolated from homemade curd.
[So] Source:PLoS One;12(8):e0184020, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One potent lactic acid bacterial strain C14 with strong antifungal activity was isolated from homemade curd. Based on morphological as well as biochemical characters and 16S rDNA sequence homology the strain was identified as Lactobacillus fermentum. It displayed a wide antimicrobial spectrum against both Gram-positive and Gram-negative pathogenic bacteria, and also against number of food spoilage, plant and human pathogenic fungi. The cell free supernatant (CFS) of the strain C14 was also effective against the fungi tested. Inhibition of radial growth of Penicillium digitatum, Trichophyton rubrum and Mucor sp. was noticed in the presence of CFS of C14 even at low concentration (1%). More than 94.3 ± 1.6% and 91.5 ± 2.2% inhibition of conidial germination of P. digitatum and Mucor sp. were noticed in the presence of 10-fold-concentrated CFS of C14. Massive deformation of the fungal mycelia was observed by SEM studies, and losses of cellular proteins and DNA are also evident upon its treatment with C14. HPLC analysis revealed the presence of phenyl lactic acid, lactic acid along with some unidentified compounds in the antifungal extract. Challenge experiment showed immense potential of the strain C14 in preventing the spoilage of bread samples caused by Mucor sp. and Bacillus subtilis. The bread samples remained fresh upto 25 days even after inoculation with Mucor sp. (3.7 × 104 spores /ml) and B. subtilis (4.6 × 104 CFU /ml). Along with the antifungal properties, the isolated lactic acid bacterial strain also showed very good antioxidant activities. Unchanged level of liver enzymes serum glutamic pyruvic transaminase and serum glutamic oxaloacetic transaminase in albino mice upon feeding with C14 also suggested non-toxic nature of the bacterial isolate.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Antibiose
Conservantes de Alimentos/farmacologia
Lactatos/farmacologia
Ácido Láctico/farmacologia
Lactobacillus fermentum/química
Leite/microbiologia
[Mh] Termos MeSH secundário: Alanina Transaminase/metabolismo
Animais
Anti-Infecciosos/isolamento & purificação
Aspartato Aminotransferases/sangue
Pão
Fermentação
Conservantes de Alimentos/isolamento & purificação
Armazenamento de Alimentos/métodos
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/crescimento & desenvolvimento
Bactérias Gram-Positivas/efeitos dos fármacos
Bactérias Gram-Positivas/crescimento & desenvolvimento
Seres Humanos
Lactatos/isolamento & purificação
Ácido Láctico/isolamento & purificação
Lactobacillus fermentum/isolamento & purificação
Masculino
Camundongos
Mucor/efeitos dos fármacos
Mucor/crescimento & desenvolvimento
Micélio/efeitos dos fármacos
Micélio/crescimento & desenvolvimento
Penicillium/efeitos dos fármacos
Penicillium/crescimento & desenvolvimento
Trichophyton/efeitos dos fármacos
Trichophyton/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Food Preservatives); 0 (Lactates); 156-05-8 (3-phenyllactic acid); 33X04XA5AT (Lactic Acid); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184020


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[PMID]:28854661
[Au] Autor:Girvin J; Whitworth RJ; Rojas LMA; Smith CM
[Ad] Endereço:USDA-APHIS-PPQ, Federal Way, WA 98003.
[Ti] Título:Resistance of Select Winter Wheat (Triticum aestivum) Cultivars to Rhopalosiphum padi (Hemiptera: Aphididae).
[So] Source:J Econ Entomol;110(4):1886-1889, 2017 Aug 01.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The bird cherry-oat aphid (Rhopalosiphum padi L.) is a global pest of wheat and vectors some of the most damaging strains of barley yellow dwarf virus (BYDV). In years of heavy R. padi infestation, R. padi and BYDV together reduce wheat yields by 30-40% in Kansas and other states of the U.S. Great Plains wheat production area. Cultivation of wheat cultivars resistant to R. padi can greatly reduce production costs and mitigate R. padi-BYDV yield losses, and increase producer profits. This study identified cultivars of hard red and soft white winter wheat with R. padi resistance that suppress R. padi populations or tolerate the effects of R. padi feeding damage. 'Pioneer (S) 25R40,' 'MFA (S) 2248,' 'Pioneer (S) 25R77,' and 'Limagrain LCS Mint' significantly reduced R. padi populations. MFA (S) 2248, Pioneer (S) 25R40, and 'Limagrain LS Wizard' exhibited tolerance expressed as significantly greater aboveground biomass. These findings are significant in that they have identified wheat cultivars currently available to producers, enabling the immediate improvement of tactics to manage R. padi and BYDV in heavily infested areas. Secondarily, these results identify cultivars that are good candidates for use in breeding and genetic analyses of arthropod resistance genes in wheat.
[Mh] Termos MeSH primário: Antibiose
Afídeos/fisiologia
Herbivoria
Triticum/fisiologia
[Mh] Termos MeSH secundário: Animais
Kansas
Luteovirus/fisiologia
Doenças das Plantas/prevenção & controle
Doenças das Plantas/virologia
Triticum/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tox164



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