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  1 / 43196 MEDLINE  
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Svidzinski, Terezinha Inez Estivalet
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[PMID]:29262785
[Au] Autor:da Silva EM; Mansano ESB; Miazima ES; Rodrigues FAV; Hernandes L; Svidzinski TIE
[Ad] Endereço:Department of Medical Mycology, State University of Maringá, Av. Colombo, 5760, C.P, Maringá, PR, 87020-900, Brazil.
[Ti] Título:Radiation used for head and neck cancer increases virulence in Candida tropicalis isolated from a cancer patient.
[So] Source:BMC Infect Dis;17(1):783, 2017 Dec 20.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Studies have shown that radiation from radiotherapy increases the yeast colonization of patients. However it is not clear, if such radiation alters the yeast itself. The aim of the present study was therefore to report the direct impact of gamma radiation on Candida tropicalis. METHODS: C. tropicalis was obtained from a patient with a carcinoma, a suspension of this yeast containing 2.0 × 10 colony forming units per milliliter was prepared. It was submitted to gamma radiation dosage similar to that used in the treatment of head and neck cancer. After a cumulative dose of 7200 cGy some virulence attributes of C. tropicalis, including macro and micromorphological characteristics, adhesion and biofilm abilities, murine experimental infection and phagocytosis resistance were evaluated on irradiated and non-irradiated yeasts. RESULTS: After irradiation the colony morphology of the yeast was altered from a ring format to a smooth appearance in most colonies. Scanning electron microscopy revealed notable differences in the structures of both these colonies and the yeast cells, with the loss of pseudohyphae following irradiation and an increase in extracellular matrix production. The adherence and biofilm production of the yeast was greater following irradiation, both in terms of the number of yeasts and total biomass production on several abiotic surfaces and TR146 cells. The phagocytic index of the irradiated yeasts was not statistically different; however, the presence of cellular debris was detected in the kidneys of infected animals. Mice infected with irradiated yeasts developed an infection at the site of the yeast inoculation, although systemic infection was unchanged. CONCLUSIONS: Our findings show for the first time that C. tropicalis, one of the most important yeasts from colonization, which cause fatal candidemia in cancer patients, is affected by gamma irradiation, with changes to its virulence profile.
[Mh] Termos MeSH primário: Candida tropicalis
Candidíase Invasiva
Neoplasias de Cabeça e Pescoço
Radioterapia/efeitos adversos
Virulência/efeitos da radiação
[Mh] Termos MeSH secundário: Biofilmes
Candida tropicalis/patogenicidade
Candida tropicalis/efeitos da radiação
Neoplasias de Cabeça e Pescoço/complicações
Neoplasias de Cabeça e Pescoço/radioterapia
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2879-6


  2 / 43196 MEDLINE  
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[PMID]:29458673
[Au] Autor:Morse DJ; Wilson MJ; Wei X; Lewis MAO; Bradshaw DJ; Murdoch C; Williams DW
[Ad] Endereço:1​Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, UK.
[Ti] Título:Denture-associated biofilm infection in three-dimensional oral mucosal tissue models.
[So] Source:J Med Microbiol;67(3):364-375, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: In vitro analyses of virulence, pathogenicity and associated host cell responses are important components in the study of biofilm infections. The Candida-related infection, denture-associated oral candidosis, affects up to 60 % of denture wearers and manifests as inflammation of palatal tissues contacting the denture-fitting surface. Commercially available three-dimensional tissue models can be used to study infection, but their use is limited for many academic research institutions, primarily because of the substantial purchase costs. The aim of this study was to develop and evaluate the use of in vitro tissue models to assess infections by biofilms on acrylic surfaces through tissue damage and Candida albicans virulence gene expression. METHODOLOGY: In vitro models were compared against commercially available tissue equivalents (keratinocyte-only, SkinEthic; full-thickness, MatTek Corporation). An in vitro keratinocyte-only tissue was produced using a cancer-derived cell line, TR146, and a full-thickness model incorporating primary fibroblasts and immortalised normal oral keratinocytes was also generated. The in vitro full-thickness tissues incorporated keratinocytes and fibroblasts, and have potential for future further development and analysis. RESULTS: Following polymicrobial infection with biofilms on acrylic surfaces, both in-house developed models were shown to provide equivalent results to the SkinEthic and MatTek models in terms of tissue damage: a significant (P<0.05) increase in LDH activity for mixed species biofilms compared to uninfected control, and no significant difference (P>0.05) in the expression of most C. albicans virulence genes when comparing tissue models of the same type. CONCLUSION: Our results confirm the feasibility and suitability of using these alternative in vitro tissue models for such analyses.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Candidíase Bucal/microbiologia
Dentaduras/microbiologia
Interações Hospedeiro-Patógeno
Mucosa Bucal/microbiologia
[Mh] Termos MeSH secundário: Candida albicans/genética
Candida albicans/patogenicidade
Candida albicans/fisiologia
Linhagem Celular
Coinfecção/microbiologia
Fibroblastos/microbiologia
Seres Humanos
Queratinócitos/microbiologia
Polimetil Metacrilato
Estomatite sob Prótese
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9011-14-7 (Polymethyl Methacrylate)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000677


  3 / 43196 MEDLINE  
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[PMID]:29458545
[Au] Autor:Song Q; Wu J; Ruan P
[Ad] Endereço:1​Department of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention, Ningbo, Zhejiang Province, PR China.
[Ti] Título:Predominance of community-associated sequence type 59 methicillin-resistant Staphylococcus aureus in a paediatric intensive care unit.
[So] Source:J Med Microbiol;67(3):408-414, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To investigate the distribution of molecular types of methicillin-resistant Staphylococcus aureus (MRSA) in a paediatric intensive care unit (PICU) according to their community-associated (CA) and hospital-associated (HA) source of acquisition, and thus assess the degree to which CA-MRSA has been introduced into the PICU. METHODOLOGY: We implemented an MRSA surveillance in a PICU during 2013-2016 and investigated the genetic diversity of the isolates retrospectively using three genetic typing methods, as well as antibiograms and virulence factor profiles.Results/Key findings. From 2684 specimens, we identified 60 MRSA isolates, 43 of which were ST59 CA-MRSA. These 43 ST59 MRSA isolates could be further subtyped into 2 clusters and 7 sporadic isolates by pulsed-field gel electrophoresis, and 3 spa types, which demonstrated the genetic diversity in ST59 MRSA. Phenotypic diversity was also demonstrated among these ST59 MRSA isolates, with 12 virulence factor profiles and 4 antibiograms being identified. Epidemiological information showed that 43 ST59 MRSA isolates were both community-associated (15 isolates) and hospital-associated (28 isolates) and caused colonization and various types of infections in different age groups of children. CONCLUSIONS: Our results show that a predominant ST59 CA-MRSA has been introduced into the PICU to a significant extent. This has caused the ST59 HA-MRSA and CA-MRSA in the PICU to be indistinguishable. Our results also demonstrate that when we are interpreting situations where the causative agents of infections focus on very limited pathogenic clones, combined typing methods and epidemiological information are needed to investigate isolates' genetic and phenotypic diversity to distinguish an outbreak from endemic cases.
[Mh] Termos MeSH primário: Infecções Comunitárias Adquiridas/microbiologia
Infecção Hospitalar/microbiologia
Unidades de Terapia Intensiva Pediátrica
Staphylococcus aureus Resistente à Meticilina/genética
Infecções Estafilocócicas/microbiologia
[Mh] Termos MeSH secundário: Adolescente
Antibacterianos/farmacologia
Técnicas de Tipagem Bacteriana
Criança
Pré-Escolar
Infecções Comunitárias Adquiridas/epidemiologia
Infecção Hospitalar/epidemiologia
DNA Bacteriano/genética
Eletroforese em Gel de Campo Pulsado
Variação Genética
Seres Humanos
Lactente
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Testes de Sensibilidade Microbiana
Tipagem de Sequências Multilocus
Estudos Retrospectivos
Análise de Sequência de DNA
Infecções Estafilocócicas/epidemiologia
Virulência/genética
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (Virulence Factors)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000693


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[PMID]:28462789
[Au] Autor:Ren J; Sang Y; Lu J; Yao YF
[Ad] Endereço:Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
[Ti] Título:Protein Acetylation and Its Role in Bacterial Virulence.
[So] Source:Trends Microbiol;25(9):768-779, 2017 Sep.
[Is] ISSN:1878-4380
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Protein acetylation is a universal post-translational modification which is found in both eukaryotes and prokaryotes. This process is achieved enzymatically by the protein acetyltransferase Pat, and nonenzymatically by metabolic intermediates (e.g., acetyl phosphate) in bacteria. Protein acetylation plays a role in bacterial chemotaxis, metabolism, DNA replication, and other cellular processes. Recently, accumulating evidence has suggested that protein acetylation might be involved in bacterial virulence because a number of bacterial virulence factors are acetylated. In this review, we summarize the progress in understanding bacterial protein acetylation and discuss how it mediates bacterial virulence.
[Mh] Termos MeSH primário: Acetiltransferases/metabolismo
Bactérias/metabolismo
Bactérias/patogenicidade
Proteínas/metabolismo
[Mh] Termos MeSH secundário: Acetilação
Bactérias/enzimologia
Proteínas de Bactérias/metabolismo
Carbono/metabolismo
Nitrogênio/metabolismo
Processamento de Proteína Pós-Traducional
Proteômica
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Proteins); 7440-44-0 (Carbon); EC 2.3.1.- (Acetyltransferases); EC 2.3.1.- (protein acyltransferase); N762921K75 (Nitrogen)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  5 / 43196 MEDLINE  
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[PMID]:29241035
[Au] Autor:Lopez CA; Skaar EP
[Ad] Endereço:Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
[Ti] Título:Crossed Wires: Interspecies Interference Blocks Pathogen Colonization.
[So] Source:Cell Host Microbe;22(6):721-723, 2017 12 13.
[Is] ISSN:1934-6069
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Commensal bacteria protect against invading pathogens using many strategies. In this issue of Cell Host & Microbe, Paharik et al. (2017) find that a commensal blocks Staphylococcus aureus colonization by producing a signal to shut down virulence.
[Mh] Termos MeSH primário: Infecções Estafilocócicas/microbiologia
Staphylococcus aureus
[Mh] Termos MeSH secundário: Seres Humanos
Simbiose
Virulência
Fatores de Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Nm] Nome de substância:
0 (Virulence Factors)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE


  6 / 43196 MEDLINE  
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[PMID]:28456668
[Au] Autor:Sarcey E; Serres A; Tindy F; Chareyre A; Ng S; Nicolas M; Vetter E; Bonnevay T; Abachin E; Mallet L
[Ad] Endereço:Sanofi Pasteur, Analytical Research and Development Department EU, Campus Mérieux-1541, Avenue Marcel Mérieux, 69280, Marcy L'Etoile, France. Electronic address: Eric.Sarcey@sanofi.com.
[Ti] Título:Quantifying low-frequency revertants in oral poliovirus vaccine using next generation sequencing.
[So] Source:J Virol Methods;246:75-80, 2017 Aug.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Spontaneous reversion to neurovirulence of live attenuated oral poliovirus vaccine (OPV) serotype 3 (chiefly involving the n.472U>C mutation), must be monitored during production to ensure vaccine safety and consistency. Mutant analysis by polymerase chain reaction and restriction enzyme cleavage (MAPREC) has long been endorsed by the World Health Organization as the preferred in vitro test for this purpose; however, it requires radiolabeling, which is no longer supported by many laboratories. We evaluated the performance and suitability of next generation sequencing (NGS) as an alternative to MAPREC. The linearity of NGS was demonstrated at revertant concentrations equivalent to the study range of 0.25%-1.5%. NGS repeatability and intermediate precision were comparable across all tested samples, and NGS was highly reproducible, irrespective of sequencing platform or analysis software used. NGS was performed on OPV serotype 3 working seed lots and monovalent bulks (n=21) that were previously tested using MAPREC, and which covered the representative range of vaccine production. Percentages of 472-C revertants identified by NGS and MAPREC were comparable and highly correlated (r≥0.80), with a Pearson correlation coefficient of 0.95585 (p<0.0001). NGS demonstrated statistically equivalent performance to that of MAPREC for quantifying low-frequency OPV serotype 3 revertants, and offers a valid alternative to MAPREC.
[Mh] Termos MeSH primário: Sequenciamento de Nucleotídeos em Larga Escala/métodos
Mutação
Vacina Antipólio Oral/genética
Poliovirus/isolamento & purificação
Poliovirus/patogenicidade
[Mh] Termos MeSH secundário: Seres Humanos
Mutação Puntual
Poliovirus/genética
Reação em Cadeia da Polimerase
Estudo de Prova de Conceito
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Poliovirus Vaccine, Oral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


  7 / 43196 MEDLINE  
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[PMID]:29346449
[Au] Autor:Chrostek E; Teixeira L
[Ad] Endereço:Instituto Gulbenkian de Ciência, Oeiras, Portugal.
[Ti] Título:Within host selection for faster replicating bacterial symbionts.
[So] Source:PLoS One;13(1):e0191530, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wolbachia is a widespread, intracellular symbiont of arthropods, able to induce reproductive distortions and antiviral protection in insects. Wolbachia can also be pathogenic, as is the case with wMelPop, a virulent variant of the endosymbiont of Drosophila melanogaster. An extensive genomic amplification of the 20kb region encompassing eight Wolbachia genes, called Octomom, is responsible for wMelPop virulence. The Octomom copy number in wMelPop can be highly variable between individual D. melanogaster flies, even when comparing siblings arising from a single female. Moreover, Octomom copy number can change rapidly between generations. These data suggest an intra-host variability in Octomom copy number between Wolbachia cells. Since wMelPop Wolbachia with different Octomom copy numbers grow at different rates, we hypothesized that selection could act on this intra-host variability. Here we tested if total Octomom copy number changes during the lifespan of individual Drosophila hosts, revealing selection for different Wolbachia populations. We performed a time course analysis of Octomom amplification in flies whose mothers were controlled for Octomom copy number. We show that despite the Octomom copy number being relatively stable it increases slightly throughout D. melanogaster adult life. This indicates that there is selection acting on the intra-host variation in the Octomom copy number over the lifespan of individual hosts. This within host selection for faster replicating bacterial symbionts may be in conflict with between host selection against highly pathogenic Wolbachia.
[Mh] Termos MeSH primário: Drosophila melanogaster/microbiologia
Interações Hospedeiro-Patógeno
Simbiose
Wolbachia/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Genes Bacterianos
Virulência/genética
Wolbachia/genética
Wolbachia/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191530


  8 / 43196 MEDLINE  
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[PMID]:29401571
[Au] Autor:Kryukov VY; Rotskaya UN; Yaroslavtseva ON; Elisaphenko EA; Duisembekov BA; Glupov VV
[Ti] Título:[Phenotypic and genetic changes of entomopathogenic ascomycete Beauveria Bassiana under passaging through various hosts].
[So] Source:Parazitologiia;51(1):3-14, 2017 Jan-Feb.
[Is] ISSN:0031-1847
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Phenotypic and genetic estimations of entomopathogenic ascomycete B.bassiana (strain Sar-31) after 6-passaging through four hosts were shown. Increasing of virulence, changes in morpho-cultural characteristics and variations in Inter Simple Sequence Repeats (ISSR) assay between initial and reisolated cultures were registered. Six passages of entomopathogenic ascomycete Beauveria bassiana (strain Sar-31) through four hosts (Galleria mellonella, Tenebrio molitor, Leptinotarsa decemlineata, Locusta migratoria) and following estimation of phenotypic and genetic differences of the initial strain and reisolated cultures were conducted. The passaging of strain through certain host led to increasing of virulence for both this host and other test-insects. Unidirectional changes of morpho-cultural characteristics: colonies pigmentation and relief strengthening, increasing of conidia production and lipolytic activity were registered in all passaged cultures. Genetic analysis with 6 ISSR markers revealed variations between initial and reisolated cultures in 3 markers. Taken together, the results of this study help us understand potential ways of fungi strains changes during epizootic process and possibilities of ISSR assay applying for investigation of pathogen transmission.
[Mh] Termos MeSH primário: Beauveria/genética
Beauveria/patogenicidade
Proteínas Fúngicas/genética
Especificidade de Hospedeiro
Esporos Fúngicos/genética
Esporos Fúngicos/patogenicidade
[Mh] Termos MeSH secundário: Animais
Beauveria/enzimologia
Beauveria/crescimento & desenvolvimento
Coleópteros/microbiologia
Proteínas Fúngicas/metabolismo
Marcadores Genéticos
Genótipo
Lipólise
Locusta migratoria/microbiologia
Repetições de Microssatélites
Mariposas/microbiologia
Fenótipo
Pigmentação/genética
Inoculações Seriadas
Esporos Fúngicos/enzimologia
Esporos Fúngicos/crescimento & desenvolvimento
Tenebrio/microbiologia
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Genetic Markers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180206
[St] Status:MEDLINE


  9 / 43196 MEDLINE  
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[PMID]:29385177
[Au] Autor:Dickel F; Münch D; Amdam GV; Mappes J; Freitak D
[Ad] Endereço:Centre of Excellence in Biological Interactions, Department of Biological and Environmental Science, University of Jyvaskyla, Jyvaskyla, Finland.
[Ti] Título:Increased survival of honeybees in the laboratory after simultaneous exposure to low doses of pesticides and bacteria.
[So] Source:PLoS One;13(1):e0191256, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recent studies of honeybees and bumblebees have examined combinatory effects of different stressors, as insect pollinators are naturally exposed to multiple stressors. At the same time the potential influences of simultaneously occurring agricultural agents on insect pollinator health remain largely unknown. Due to different farming methods, and the drift of applied agents and manure, pollinators are most probably exposed to insecticides but also bacteria from organic fertilizers at the same time. We orally exposed honeybee workers to sub-lethal doses of the insecticide thiacloprid and two strains of the bacterium Enterococcus faecalis, which can occur in manure from farming animals. Our results show that under laboratory conditions the bees simultaneously exposed to the a bacterium and the pesticide thiacloprid thiacloprid had significant higher survival rates 11 days post exposure than the controls, which surprisingly showed the lowest survival. Bees that were exposed to diet containing thiacloprid showed decreased food intake. General antibacterial activity is increased by the insecticide and the bacteria, resulting in a higher immune response observed in treated individuals compared to control individuals. We thus propose that caloric restriction through behavioural and physiological adaptations may have mediated an improved survival and stress resistance in our tests. However, the decreased food consumption could in long-term also result in possible negative effects at colony level. Our study does not show an additive negative impact of sub-lethal insecticide and bacteria doses, when tested under laboratory conditions. In contrast, we report seemingly beneficial effects of simultaneous exposure of bees to agricultural agents, which might demonstrate a surprising biological capacity for coping with stressors, possibly through hormetic regulation.
[Mh] Termos MeSH primário: Abelhas/efeitos dos fármacos
Abelhas/microbiologia
Enterococcus faecalis/patogenicidade
Inseticidas/toxicidade
Neonicotinoides/toxicidade
Tiazinas/toxicidade
[Mh] Termos MeSH secundário: Animais
Abelhas/fisiologia
Ingestão de Alimentos/efeitos dos fármacos
Hormese
Inseticidas/administração & dosagem
Neonicotinoides/administração & dosagem
Polinização
Estresse Fisiológico
Tiazinas/administração & dosagem
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insecticides); 0 (Neonicotinoids); 0 (Thiazines); DSV3A944A4 (thiacloprid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191256


  10 / 43196 MEDLINE  
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[PMID]:29351318
[Au] Autor:Sigaúque B; Kobayashi M; Vubil D; Nhacolo A; Chaúque A; Moaine B; Massora S; Mandomando I; Nhampossa T; Bassat Q; Pimenta F; Menéndez C; Carvalho MDG; Macete E; Schrag SJ
[Ad] Endereço:Centro de Investigação em Saúde de Manhiça, Maputo, Mozambique.
[Ti] Título:Invasive bacterial disease trends and characterization of group B streptococcal isolates among young infants in southern Mozambique, 2001-2015.
[So] Source:PLoS One;13(1):e0191193, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Maternal group B streptococcal (GBS) vaccines under development hold promise to prevent GBS disease in young infants. Sub-Saharan Africa has the highest estimated disease burden, although data on incidence and circulating strains are limited. We described invasive bacterial disease (IBD) trends among infants <90 days in rural Mozambique during 2001-2015, with a focus on GBS epidemiology and strain characteristics. METHODS: Community-level birth and mortality data were obtained from Manhiça's demographic surveillance system. IBD cases were captured through ongoing surveillance at Manhiça district hospital. Stored GBS isolates from cases underwent serotyping by multiplex PCR, antimicrobial susceptibility testing, and whole genome sequencing. RESULTS: There were 437 IBD cases, including 57 GBS cases. Significant declines in overall IBD, neonatal mortality, and stillbirth rates were observed (P<0.0001), but not for GBS (P = 0.17). In 2015, GBS was the leading cause of young infant IBD (2.7 per 1,000 live births). Among 35 GBS isolates available for testing, 31 (88.6%) were highly related serotype III isolates within multilocus sequence types (STs) 17 (68.6%) or 109 (20.0%). All seven ST109 isolates (21.9%) had elevated minimum inhibitory concentration (MIC) to penicillin (≥0.12 µg/mL) associated with penicillin-binding protein (PBP) 2x substitution G398A. Epidemiologic and molecular data suggest this is a well-established clone. CONCLUSION: A notable young infant GBS disease burden persisted despite improvements in overall maternal and neonatal health. We report an established strain with pbp2x point mutation, a first-step mutation associated with reduced penicillin susceptibility within a well-known virulent lineage in rural Mozambique. Our findings further underscores the need for non-antibiotic GBS prevention strategies.
[Mh] Termos MeSH primário: Infecções Bacterianas/epidemiologia
Infecções Estreptocócicas/epidemiologia
Infecções Estreptocócicas/microbiologia
Streptococcus agalactiae
[Mh] Termos MeSH secundário: Idade de Início
Feminino
Genoma Bacteriano
Seres Humanos
Imunidade Materno-Adquirida
Incidência
Lactente
Recém-Nascido
Masculino
Testes de Sensibilidade Microbiana
Moçambique/epidemiologia
Reação em Cadeia da Polimerase Multiplex
Resistência às Penicilinas/genética
Proteínas de Ligação às Penicilinas/genética
Mutação Puntual
Gravidez
Estudos Retrospectivos
Sorotipagem
Infecções Estreptocócicas/prevenção & controle
Vacinas Estreptocócicas/farmacologia
Streptococcus agalactiae/efeitos dos fármacos
Streptococcus agalactiae/genética
Streptococcus agalactiae/isolamento & purificação
Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Penicillin-Binding Proteins); 0 (Streptococcal Vaccines); 128284-03-7 (PBP 2x protein, Streptococcus)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191193



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