[PMID]: | 28724546 |
[Au] Autor: | Wang L; Jacobs JP; Lagishetty V; Yuan PQ; Wu SV; Million M; Reeve JR; Pisegna JR; Taché Y |
[Ad] Endereço: | CURE/Digestive Diseases Research Center and Center for Neurobiology of Stress and Resilience, Department of Medicine, Vatche and Tamar Manoukian Digestive Diseases Division, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California; and Lixinw@ucla.edu. |
[Ti] Título: | High-protein diet improves sensitivity to cholecystokinin and shifts the cecal microbiome without altering brain inflammation in diet-induced obesity in rats. |
[So] Source: | Am J Physiol Regul Integr Comp Physiol;313(4):R473-R486, 2017 Oct 01. |
[Is] ISSN: | 1522-1490 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | High-protein diet (HPD) curtails obesity and/or fat mass, but it is unknown whether it reverses neuroinflammation or alters glucose levels, CCK sensitivity, and gut microbiome in rats fed a Western diet (WD)-induced obesity (DIO). Male rats fed a WD (high fat and sugar) for 12 wk were switched to a HPD for 6 wk. Body composition, food intake, meal pattern, sensitivity to intraperitoneal CCK-8S, blood glucose, brain signaling, and cecal microbiota were assessed. When compared with a normal diet, WD increased body weight (9.3%) and fat mass (73.4%). CCK-8S (1.8 or 5.2 nmol/kg) did not alter food intake and meal pattern in DIO rats. Switching to a HPD for 6 wk reduced fat mass (15.7%) with a nonsignificantly reduced body weight gain, normalized blood glucose, and decreased feeding after CCK-8S. DIO rats on the WD or switched to a HPD showed comparable microbial diversity. However, in HPD versus WD rats, there was enrichment of 114 operational taxonomic units (OTUs) and depletion of 188 OTUs. Of those, (enriched on a HPD), an unclassified Clostridiales, a member of the RF39 order, and a were significantly associated with fat mass. The WD increased cytokine expression in the hypothalamus and dorsal medulla that was unchanged by switching to HPD. These data indicate that HPD reduces body fat and restores glucose homeostasis and CCK sensitivity, while not modifying brain inflammation. In addition, expansion of cecal correlated to fat mass loss may represent a potential peripheral mechanism of HPD beneficial effects. |
[Mh] Termos MeSH primário: |
Encéfalo/efeitos dos fármacos Ceco/efeitos dos fármacos Colecistocinina/farmacologia Proteínas na Dieta/farmacologia Encefalite/metabolismo Microbiota/efeitos dos fármacos Obesidade/microbiologia
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[Mh] Termos MeSH secundário: |
Animais Glicemia/metabolismo Composição Corporal/efeitos dos fármacos Peso Corporal/efeitos dos fármacos Encéfalo/metabolismo Ceco/metabolismo Ceco/microbiologia Citocinas/metabolismo Dieta Ocidental Ingestão de Alimentos/efeitos dos fármacos Masculino Obesidade/metabolismo Ratos Ratos Sprague-Dawley
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Blood Glucose); 0 (Cytokines); 0 (Dietary Proteins); 9011-97-6 (Cholecystokinin) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171108 |
[Lr] Data última revisão:
| 171108 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170721 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1152/ajpregu.00105.2017 |
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