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Pesquisa : G07.345.249.845.400 [Categoria DeCS]
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  1 / 1170 MEDLINE  
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[PMID]:29324818
[Au] Autor:Fujii N; Miyabayashi S; Sugita T; Kobayashi A; Yamazaki C; Miyazawa Y; Kamada M; Kasahara H; Osada I; Shimazu T; Fusejima Y; Higashibata A; Yamazaki T; Ishioka N; Takahashi H
[Ad] Endereço:Graduate School of Life Sciences, Tohoku University, Katahira, Aoba-ku, Sendai, Japan.
[Ti] Título:Root-tip-mediated inhibition of hydrotropism is accompanied with the suppression of asymmetric expression of auxin-inducible genes in response to moisture gradients in cucumber roots.
[So] Source:PLoS One;13(1):e0189827, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In cucumber seedlings, gravitropism interferes with hydrotropism, which results in the nearly complete inhibition of hydrotropism under stationary conditions. However, hydrotropic responses are induced when the gravitropic response in the root is nullified by clinorotation. Columella cells in the root cap sense gravity, which induces the gravitropic response. In this study, we found that removing the root tip induced hydrotropism in cucumber roots under stationary conditions. The application of auxin transport inhibitors to cucumber seedlings under stationary conditions suppressed the hydrotropic response induced by the removal of the root tip. To investigate the expression of genes related to hydrotropism in de-tipped cucumber roots, we conducted transcriptome analysis of gene expression by RNA-Seq using seedlings exhibiting hydrotropic and gravitropic responses. Of the 21 and 45 genes asymmetrically expressed during hydrotropic and gravitropic responses, respectively, five genes were identical. Gene ontology (GO) analysis indicated that the category auxin-inducible genes was significantly enriched among genes that were more highly expressed in the concave side of the root than the convex side during hydrotropic or gravitropic responses. Reverse transcription followed by quantitative polymerase chain reaction (RT-qPCR) analysis revealed that root hydrotropism induced under stationary conditions (by removing the root tip) was accompanied by the asymmetric expression of several auxin-inducible genes. However, intact roots did not exhibit the asymmetric expression patterns of auxin-inducible genes under stationary conditions, even in the presence of a moisture gradient. These results suggest that the root tip inhibits hydrotropism by suppressing the induction of asymmetric auxin distribution. Auxin transport and distribution not mediated by the root tip might play a role in hydrotropism in cucumber roots.
[Mh] Termos MeSH primário: Cucumis sativus/genética
Regulação da Expressão Gênica de Plantas/fisiologia
Gravitropismo/fisiologia
Ácidos Indolacéticos/metabolismo
Raízes de Plantas/fisiologia
[Mh] Termos MeSH secundário: Cucumis sativus/crescimento & desenvolvimento
Genes de Plantas
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Transcriptoma
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Indoleacetic Acids); 059QF0KO0R (Water)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189827


  2 / 1170 MEDLINE  
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[PMID]:27773568
[Au] Autor:Rakusová H; Abbas M; Han H; Song S; Robert HS; Friml J
[Ad] Endereço:Institute of Science and Technology (IST) Austria, 3400 Klosterneuburg, Austria; Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
[Ti] Título:Termination of Shoot Gravitropic Responses by Auxin Feedback on PIN3 Polarity.
[So] Source:Curr Biol;26(22):3026-3032, 2016 Nov 21.
[Is] ISSN:1879-0445
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plants adjust their growth according to gravity. Gravitropism involves gravity perception, signal transduction, and asymmetric growth response, with organ bending as a consequence [1]. Asymmetric growth results from the asymmetric distribution of the plant-specific signaling molecule auxin [2] that is generated by lateral transport, mediated in the hypocotyl predominantly by the auxin transporter PIN-FORMED3 (PIN3) [3-5]. Gravity stimulation polarizes PIN3 to the bottom sides of endodermal cells, correlating with increased auxin accumulation in adjacent tissues at the lower side of the stimulated organ, where auxin induces cell elongation and, hence, organ bending. A curvature response allows the hypocotyl to resume straight growth at a defined angle [6], implying that at some point auxin symmetry is restored to prevent overbending. Here, we present initial insights into cellular and molecular mechanisms that lead to the termination of the tropic response. We identified an auxin feedback on PIN3 polarization as underlying mechanism that restores symmetry of the PIN3-dependent auxin flow. Thus, two mechanistically distinct PIN3 polarization events redirect auxin fluxes at different time points of the gravity response: first, gravity-mediated redirection of PIN3-mediated auxin flow toward the lower hypocotyl side, where auxin gradually accumulates and promotes growth, and later PIN3 polarization to the opposite cell side, depleting this auxin maximum to end the bending. Accordingly, genetic or pharmacological interference with the late PIN3 polarization prevents termination of the response and leads to hypocotyl overbending. This observation reveals a role of auxin feedback on PIN polarity in the termination of the tropic response.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/fisiologia
Gravitropismo
Sensação Gravitacional
Ácidos Indolacéticos/metabolismo
Reguladores de Crescimento de Planta/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/metabolismo
Retroalimentação Fisiológica
Brotos de Planta/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Indoleacetic Acids); 0 (PIN3 protein, Arabidopsis); 0 (Plant Growth Regulators)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  3 / 1170 MEDLINE  
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[PMID]:27649449
[Au] Autor:Möller BK; Xuan W; Beeckman T
[Ad] Endereço:Department of Plant Systems Biology, VIB, Technologiepark 927, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium.
[Ti] Título:Dynamic control of lateral root positioning.
[So] Source:Curr Opin Plant Biol;35:1-7, 2017 Feb.
[Is] ISSN:1879-0356
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In dicot root systems, lateral roots are in general regularly spaced along the longitudinal axis of the primary root to facilitate water and nutrient uptake. Recently, recurrent programmed cell death in the root cap of the growing root has been implicated in lateral root spacing. The root cap contains an auxin source that modulates lateral root patterning. Periodic release of auxin by dying root cap cells seems to trigger lateral root specification at regular intervals. However, it is currently unclear through which molecular mechanisms auxin restricts lateral root specification to specific cells along the longitudinal and radial axes of the root, or how environmental signals impact this process.
[Mh] Termos MeSH primário: Arabidopsis/crescimento & desenvolvimento
Raízes de Plantas/crescimento & desenvolvimento
Tropismo
[Mh] Termos MeSH secundário: Gravitropismo
Coifa/crescimento & desenvolvimento
Água/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE


  4 / 1170 MEDLINE  
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[PMID]:27263083
[Au] Autor:Ibragimova NN; Ageeva MV; Gorshkova TA
[Ad] Endereço:Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Lobachevsky Str. 2/31, Kazan, 420111, Russia. nibra@yandex.ru.
[Ti] Título:Development of gravitropic response: unusual behavior of flax phloem G-fibers.
[So] Source:Protoplasma;254(2):749-762, 2017 Mar.
[Is] ISSN:1615-6102
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The major mechanism of gravitropism that is discussed for herbal plants is based on the nonuniform elongation of cells located on the opposite stem sides, occurring in the growing zone of an organ. However, gravitropic response of flax (Linum usitatissimum L.) is well-pronounced in the lower half of developing stem, which has ceased elongation long in advance of plant inclination. We have analyzed the stem curvature region by various approaches of microscopy and found the undescribed earlier significant modifications in primary phloem fibers that have constitutively developed G-layer. In fibers on the pulling stem side, cell portions were widened with formation of "bottlenecks" between them, leading to the "sausage-like" shape of a cell. Lumen diameter in fiber widening increased, while cell wall thickness decreased. Callose was deposited in proximity to bottlenecks and sometimes totally occluded their lumen. Structure of fiber cell wall changed considerably, with formation of breaks between G- and S-layers. Thick fibrillar structures that were revealed in fiber cell wall by light microscopy got oblique orientation instead of parallel to the fiber axis one in control plants. The described changes occurred at various combinations of gravitational and mechanical stimuli. Thus, phloem fibers with constitutively formed gelatinous cell wall, located in nonelongating parts of herbal plant, are involved in gravitropism and may become an important element in general understanding of the gravity effects on plants. We suggest flax phloem fibers as the model system to study the mechanism of plant position correction, including signal perception and transduction.
[Mh] Termos MeSH primário: Linho/fisiologia
Gravitropismo/fisiologia
Floema/fisiologia
[Mh] Termos MeSH secundário: Parede Celular/metabolismo
Parede Celular/ultraestrutura
Linho/crescimento & desenvolvimento
Linho/ultraestrutura
Glucanos/metabolismo
Floema/anatomia & histologia
Floema/citologia
Floema/ultraestrutura
Caules de Planta/fisiologia
Caules de Planta/ultraestrutura
Xilema/fisiologia
Xilema/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 9064-51-1 (callose)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160606
[St] Status:MEDLINE
[do] DOI:10.1007/s00709-016-0985-8


  5 / 1170 MEDLINE  
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[PMID]:27758895
[Au] Autor:Kim J; Song K; Park E; Kim K; Bae G; Choi G
[Ad] Endereço:Department of Biological Sciences, KAIST, Daejeon 34141, Korea.
[Ti] Título:Epidermal Phytochrome B Inhibits Hypocotyl Negative Gravitropism Non-Cell-Autonomously.
[So] Source:Plant Cell;28(11):2770-2785, 2016 Nov.
[Is] ISSN:1532-298X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Seedling hypocotyls display negative gravitropism in the dark but agravitropism in the light. The Arabidopsis thaliana pif quadruple mutant (pifQ), which lacks four PHYTOCHROME-INTERACTING FACTORS (PIFs), is agravitropic in the dark. Endodermis-specific expression of PIF1 rescues gravitropism in pifQ mutant seedlings. Since phytochromes induce light responses by inhibiting PIFs and the COP1-SPA ubiquitin E3 ligase complex in the nucleus, we asked whether phyB can cell autonomously inhibit hypocotyl negative gravitropism in the endodermis. We found that while epidermis-specific expression of PHYB rescues hypocotyl negative gravitropism and all other phyB mutant phenotypes, endodermis-specific expression of PHYB does not. Epidermal phyB induces the phosphorylation and degradation of endodermal PIFs in response to red light. This induces a global gene expression pattern similar to that induced by red light treatment of seedlings expressing PHYB under the control of its own endogenous promoter. Our results imply that epidermal phyB generates an unidentified mobile signal that travels to the endodermis where it promotes PIF degradation and inhibits hypocotyl negative gravitropism.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/metabolismo
Hipocótilo/metabolismo
Fitocromo B/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/genética
Proteínas de Arabidopsis/genética
Regulação da Expressão Gênica de Plantas/genética
Regulação da Expressão Gênica de Plantas/fisiologia
Gravitropismo/genética
Gravitropismo/fisiologia
Hipocótilo/genética
Fitocromo B/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (PHYB protein, Arabidopsis); 136250-22-1 (Phytochrome B)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE


  6 / 1170 MEDLINE  
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[PMID]:27733332
[Au] Autor:Wu D; Huang LZ; Gao J; Wang YH
[Ad] Endereço:State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
[Ti] Título:The molecular mechanism of plant gravitropism.
[So] Source:Yi Chuan;38(7):589-602, 2016 07 20.
[Is] ISSN:0253-9772
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Gravity is an important environmental factor that regulates plant growth and morphogenesis. In response to gravity stimulus, plants can set the optimum angle between the organs and the gravity vector. Plant gravitropism is divided into four sequential steps, including gravity perception, signal transduction, asymmetrical distribution of auxin, and organ curvature. In recent years, large numbers of mutants with defective gravitropism have been identified and genes involved in the regulation of gravitropism have been functionally characterized. In particular, progress has been achieved on elucidating the molecular mechanisms of gravity perception and asymmetrical distribution of auxin. As one of the most important strategies for plant to adapt environmental changes, gravitropism is also involved in the regulation of rice plant architecture and grain yield through modulating rice tiller angle. Therefore, the investigation of plant gravitropism not only contributes to decipher the regulatory mechanisms of plant growth and development, but also helps to guide the genetic improvement of crop architecture. However, the molecular mechanisms and regulatory network of gravitropism remain to be elusive. In this review, we focus on recent progress on elucidating molecular mechanisms underlying gravitropism and its involvement in regulating rice tiller angle, which is an important agronomic trait that determines rice plant architecture and thus grain yields.
[Mh] Termos MeSH primário: Gravitropismo/genética
Desenvolvimento Vegetal
[Mh] Termos MeSH secundário: Cálcio/metabolismo
Oryza/genética
Oryza/crescimento & desenvolvimento
Reguladores de Crescimento de Planta/análise
Transdução de Sinais/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Plant Growth Regulators); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161014
[St] Status:MEDLINE


  7 / 1170 MEDLINE  
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[PMID]:27728837
[Au] Autor:Dümmer M; Michalski C; Essen LO; Rath M; Galland P; Forreiter C
[Ad] Endereço:Fachbereich Biologie, Philipps-Universität Marburg, Karl-von-Frisch Str. 8, D-35032 Marburg, Germany. Electronic address: duemmer@Staff.Uni-Marburg.DE.
[Ti] Título:EHB1 and AGD12, two calcium-dependent proteins affect gravitropism antagonistically in Arabidopsis thaliana.
[So] Source:J Plant Physiol;206:114-124, 2016 Nov 01.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The ADP-RIBOSYLATION FACTOR GTPase-ACTIVATING PROTEIN (AGD) 12, a member of the ARF-GAP protein family, affects gravitropism in Arabidopsis thaliana. A loss-of-function mutant lacking AGD12 displayed diminished gravitropism in roots and hypocotyls indicating that both organs are affected by this regulator. AGD12 is structurally related to ENHANCED BENDING (EHB) 1, previously described as a negative effector of gravitropism. In contrast to agd12 mutants, ehb1 loss-of function seedlings displayed enhanced gravitropic bending. While EHB1 and AGD12 both possess a C-terminal C2/CaLB-domain, EHB1 lacks the N-terminal ARF-GAP domain present in AGD12. Subcellular localization analysis using Brefeldin A indicated that both proteins are elements of the trans Golgi network. Physiological analyses provided evidence that gravitropic signaling might operate via an antagonistic interaction of ARF-GAP (AGD12) and EHB1 in their Ca -activated states.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/fisiologia
Cálcio/farmacologia
Proteínas Ativadoras de GTPase/metabolismo
Gravitropismo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/efeitos dos fármacos
Proteínas de Arabidopsis/química
Estiolamento/efeitos dos fármacos
Proteínas Ativadoras de GTPase/química
Meristema/efeitos dos fármacos
Meristema/fisiologia
Mutação/genética
Domínios Proteicos
Plântulas/efeitos dos fármacos
Plântulas/fisiologia
Frações Subcelulares/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AGD12 protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (EHB1 protein, Arabidopsis); 0 (GTPase-Activating Proteins); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170827
[Lr] Data última revisão:
170827
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161012
[St] Status:MEDLINE


  8 / 1170 MEDLINE  
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[PMID]:27535793
[Au] Autor:Krieger G; Shkolnik D; Miller G; Fromm H
[Ad] Endereço:Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel (G.K., D.S., H.F.); andMina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 5290002, Israel (G.M.).
[Ti] Título:Reactive Oxygen Species Tune Root Tropic Responses.
[So] Source:Plant Physiol;172(2):1209-1220, 2016 Oct.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The default growth pattern of primary roots of land plants is directed by gravity. However, roots possess the ability to sense and respond directionally to other chemical and physical stimuli, separately and in combination. Therefore, these root tropic responses must be antagonistic to gravitropism. The role of reactive oxygen species (ROS) in gravitropism of maize and Arabidopsis (Arabidopsis thaliana) roots has been previously described. However, which cellular signals underlie the integration of the different environmental stimuli, which lead to an appropriate root tropic response, is currently unknown. In gravity-responding roots, we observed, by applying the ROS-sensitive fluorescent dye dihydrorhodamine-123 and confocal microscopy, a transient asymmetric ROS distribution, higher at the concave side of the root. The asymmetry, detected at the distal elongation zone, was built in the first 2 h of the gravitropic response and dissipated after another 2 h. In contrast, hydrotropically responding roots show no transient asymmetric distribution of ROS Decreasing ROS levels by applying the antioxidant ascorbate, or the ROS-generation inhibitor diphenylene iodonium attenuated gravitropism while enhancing hydrotropism. Arabidopsis mutants deficient in Ascorbate Peroxidase 1 showed attenuated hydrotropic root bending. Mutants of the root-expressed NADPH oxidase RBOH C, but not rbohD, showed enhanced hydrotropism and less ROS in their roots apices (tested in tissue extracts with Amplex Red). Finally, hydrostimulation prior to gravistimulation attenuated the gravistimulated asymmetric ROS and auxin signals that are required for gravity-directed curvature. We suggest that ROS, presumably H O , function in tuning root tropic responses by promoting gravitropism and negatively regulating hydrotropism.
[Mh] Termos MeSH primário: Arabidopsis/fisiologia
Gravitropismo/fisiologia
Raízes de Plantas/crescimento & desenvolvimento
Espécies Reativas de Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Antioxidantes/farmacologia
Arabidopsis/genética
Arabidopsis/metabolismo
Ácido Ascórbico/farmacologia
Gravitropismo/efeitos dos fármacos
Gravitropismo/genética
Isoenzimas/genética
Isoenzimas/metabolismo
Microscopia Confocal
Mutação
NADPH Oxidases/genética
NADPH Oxidases/metabolismo
Oniocompostos/farmacologia
Raízes de Plantas/genética
Raízes de Plantas/metabolismo
Tropismo/efeitos dos fármacos
Tropismo/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Isoenzymes); 0 (Onium Compounds); 0 (Reactive Oxygen Species); 6HJ411TU98 (diphenyleneiodonium); EC 1.6.3.- (NADPH Oxidases); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160819
[St] Status:MEDLINE


  9 / 1170 MEDLINE  
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[PMID]:27490179
[Au] Autor:Araniti F; Graña E; Krasuska U; Bogatek R; Reigosa MJ; Abenavoli MR; Sánchez-Moreiras AM
[Ad] Endereço:Dipartimento di AGRARIA, Università Mediterranea di Reggio Calabria, Facoltà di Agraria - Salita Melissari, Lotto-D, I-89124, Reggio Calabria RC, Italy.
[Ti] Título:Loss of Gravitropism in Farnesene-Treated Arabidopsis Is Due to Microtubule Malformations Related to Hormonal and ROS Unbalance.
[So] Source:PLoS One;11(8):e0160202, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mode of action of farnesene, a volatile sesquiterpene commonly found in the essential oils of several plants, was deeply studied on the model species Arabidopsis thaliana. The effects of farnesene on the Arabidopsis root morphology were evaluated by different microscopic techniques. As well, microtubules immunolabeling, phytohormone measurements and ROS staining helped us to elucidate the single or multi-modes of action of this sesquiterpene on plant metabolism. Farnesene-treated roots showed a strong growth inhibition and marked modifications on morphology, important tissue alterations, cellular damages and anisotropic growth. Left-handed growth of farnesene-treated roots, reverted by taxol (a known microtubule stabilizer), was related to microtubule condensation and disorganization. As well, the inhibition of primary root growth, lateral root number, lateral root length, and both root hairs length and density could be explained by the strong increment in ethylene production and auxin content detected in farnesene-treated seedlings. Microtubule alteration and hormonal unbalance appear as important components in the mode of action of farnesene and confirm the strong phytotoxic potential of this sesquiterpene.
[Mh] Termos MeSH primário: Arabidopsis/efeitos dos fármacos
Gravitropismo
Microtúbulos/metabolismo
Reguladores de Crescimento de Planta/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Sesquiterpenos/farmacologia
[Mh] Termos MeSH secundário: Arabidopsis/crescimento & desenvolvimento
Arabidopsis/metabolismo
Etilenos/metabolismo
Peróxido de Hidrogênio/análise
Ácidos Indolacéticos/metabolismo
Microscopia Eletrônica de Transmissão
Óxido Nítrico/metabolismo
Paclitaxel/farmacologia
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/fisiologia
Plântulas/efeitos dos fármacos
Plântulas/crescimento & desenvolvimento
Superóxidos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenes); 0 (Indoleacetic Acids); 0 (Plant Growth Regulators); 0 (Reactive Oxygen Species); 0 (Sesquiterpenes); 11062-77-4 (Superoxides); 31C4KY9ESH (Nitric Oxide); 91GW059KN7 (ethylene); BBX060AN9V (Hydrogen Peroxide); P88XT4IS4D (Paclitaxel)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160805
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0160202


  10 / 1170 MEDLINE  
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[PMID]:27473572
[Au] Autor:Zou JJ; Zheng ZY; Xue S; Li HH; Wang YR; Le J
[Ad] Endereço:Key Laboratory of Plant Molecular Physiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
[Ti] Título:The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism.
[So] Source:J Exp Bot;67(18):5325-5337, 2016 Oct.
[Is] ISSN:1460-2431
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Gravitropism is vital for shaping directional plant growth in response to the forces of gravity. Signals perceived in the gravity-sensing cells can be converted into biochemical signals and transmitted. Sedimentation of amyloplasts in the columella cells triggers asymmetric auxin redistribution in root tips, leading to downward root growth. The actin cytoskeleton is thought to play an important role in root gravitropism, although the molecular mechanism has not been resolved. DISTORTED1 (DIS1) encodes the ARP3 subunit of the Arabidopsis Actin-Related Protein 2/3 (ARP2/3) complex, and the ARP3/DIS1 mutant dis1-1 showed delayed root curvature after gravity stimulation. Microrheological analysis revealed that the high apparent viscosity within dis1-1 central columella cells is closely associated with abnormal movement trajectories of amyloplasts. Analysis using a sensitive auxin input reporter DII-VENUS showed that asymmetric auxin redistribution was reduced in the root tips of dis1-1, and the actin-disrupting drug Latrunculin B increased the asymmetric auxin redistribution. An uptake assay using the membrane-selective dye FM4-64 indicated that endocytosis was decelerated in dis1-1 root epidermal cells. Treatment and wash-out with Brefeldin A, which inhibits protein transport from the endoplasmic reticulum to the Golgi apparatus, showed that cycling of the auxin-transporter PIN-FORMED (PIN) proteins to the plasma membrane was also suppressed in dis1-1 roots. The results reveal that ARP3/DIS1 acts in root gravitropism by affecting amyloplast sedimentation and PIN-mediated polar auxin transport through regulation of PIN protein trafficking.
[Mh] Termos MeSH primário: Proteína 3 Relacionada a Actina/fisiologia
Proteínas de Arabidopsis/fisiologia
Gravitropismo/fisiologia
Ácidos Indolacéticos/metabolismo
Reguladores de Crescimento de Planta/fisiologia
Raízes de Plantas/fisiologia
Plastídeos/fisiologia
[Mh] Termos MeSH secundário: Proteína 3 Relacionada a Actina/genética
Actinas/metabolismo
Arabidopsis/genética
Arabidopsis/fisiologia
Proteínas de Arabidopsis/genética
Gravitropismo/genética
Microscopia Confocal
Plastídeos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actin-Related Protein 3); 0 (Actins); 0 (Arabidopsis Proteins); 0 (DIS1 protein, Arabidopsis); 0 (Indoleacetic Acids); 0 (Plant Growth Regulators)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160731
[St] Status:MEDLINE



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