Base de dados : MEDLINE
Pesquisa : G07.345.500.325 [Categoria DeCS]
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  1 / 22621 MEDLINE  
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[PMID]:15490885
[Au] Autor:Yang ZT; Shen W; Deng JX
[Ad] Endereço:Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. yangzhengtian@yahoo.com.cn
[Ti] Título:[Nuclear reprogramming of somatic nuclear transfer embryos].
[So] Source:Yi Chuan Xue Bao;31(6):641-6, 2004 Jun.
[Is] ISSN:0379-4172
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Although the cloned animals have been successfully generated in a number of mammalian species, there are still many problems about this technology. The developmental aberrancies include a high rate of abortion during early gestation and high rate of perinatal death. The main cause of these problems may be attributed to the epigenetic reprogramming of somatic donor genome. During mammalian embryonic development, DNA methylation is an essential process in the regulation of transcription. There are many aberrant methylation in various genomic regions of cloned embryos. The gene imprinting of cloned embryos are also abnormal.
[Mh] Termos MeSH primário: Clonagem de Organismos
Metilação de DNA
Impressão Genômica
Técnicas de Transferência Nuclear
[Mh] Termos MeSH secundário: Desenvolvimento Embrionário e Fetal
Regulação da Expressão Gênica no Desenvolvimento
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Em] Mês de entrada:0411
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:041020
[St] Status:MEDLINE


  2 / 22621 MEDLINE  
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[PMID]:15481861
[Au] Autor:Adam AA; Takahashi Y; Katagiri S; Nagano M
[Ad] Endereço:Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
[Ti] Título:Effects of oxygen tension in the gas atmosphere during in vitro maturation, in vitro fertilization and in vitro culture on the efficiency of in vitro production of mouse embryos.
[So] Source:Jpn J Vet Res;52(2):77-84, 2004 Aug.
[Is] ISSN:0047-1917
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Effects of oxygen (O2) tension in the gas atmosphere during in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC) on the efficiency of in vitro production of mouse embryos were examined. Mouse oocytes recovered from large antral follicles were subjected to IVM in Waymouth medium for 15, 16 and 17 hr under 5 or 20% O2 and then subjected to IVF and IVC under 5 or 20% O2 tension. Lowering the O2 tension in the gas atmosphere for IVM from 20 to 5% improved the cleavage rate after IVF when the oocytes were subjected to IVM for 15 hr; however, no improvement in the cleavage rate was observed when the culture period for IVM was extended to 16 and 17 hr. Lowering the O2 tension to 5% for IVM and IVC improved the development of the cleaved oocytes to the blastocyst stage, regardless of the culture period for IVM. However, the O2 tension for IVF had no remarkable effect on the subsequent embryonic development. These results demonstrate that 5% O2 is superior to 20% O2 for IVM and IVC, and suggest that 20% O2 for IVM may delay oocyte maturation and/or the acquisition of fertilizability and impair the developmental competence of oocytes.
[Mh] Termos MeSH primário: Embrião de Mamíferos/efeitos dos fármacos
Embrião de Mamíferos/fisiologia
Camundongos/embriologia
Oócitos/efeitos dos fármacos
Oxigênio/farmacologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Cultura de Células/métodos
Divisão Celular/efeitos dos fármacos
Divisão Celular/fisiologia
Relação Dose-Resposta a Droga
Desenvolvimento Embrionário e Fetal/efeitos dos fármacos
Desenvolvimento Embrionário e Fetal/fisiologia
Feminino
Fertilização In Vitro/veterinária
Masculino
Camundongos Endogâmicos ICR
Oócitos/metabolismo
Oócitos/fisiologia
Oxigênio/metabolismo
Distribuição Aleatória
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
S88TT14065 (Oxygen)
[Em] Mês de entrada:0411
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:041016
[St] Status:MEDLINE


  3 / 22621 MEDLINE  
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[PMID]:15473488
[Au] Autor:Rabkin-Aikawa E; Farber M; Aikawa M; Schoen FJ
[Ad] Endereço:Department of Pathology, Department of Medicine, Brigham and Women's Hospital, and Harvard Medical School, Boston, MA 02115, USA.
[Ti] Título:Dynamic and reversible changes of interstitial cell phenotype during remodeling of cardiac valves.
[So] Source:J Heart Valve Dis;13(5):841-7, 2004 Sep.
[Is] ISSN:0966-8519
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIM OF THE STUDY: The roles of cardiac valvular interstitial cells (VIC) in extracellular matrix remodeling in fetal development, adaptation and response to injury are largely unknown. METHODS: The phenotype of VIC was studied in health (normal adult human and sheep), development (fetal human and sheep), disease (human mitral valves with myxomatous degeneration), adaptation (clinical pulmonary to aortic valve autografts) and tissue-engineered heart valves matured in vitro and remodeled in vivo. Cell phenotype was assessed using expression of vimentin (V), alpha-smooth muscle actin (SMA, A), matrix metalloproteinase (MMP)-13/collagenase-3 (M), and SMemb (S). RESULTS: VIC in normal adult valves were predominantly quiescent fibroblasts immunoreactive to vimentin (89.7 +/- 2.5%), but not MMP-13 or SMemb, with only 2.5 +/- 0.4% of alpha-SMA-positive cells ('normal/quiescent' phenotype: V+/A-/M-/S-). In contrast, fetal VIC were mostly activated myofibroblasts ('developing/activated' phenotype: V+/A+/M+/S+), with 62.1 +/- 5.0% of cells staining positive for alpha-SMA. VIC in myxomatous valves, short-term autografts and engineered valves in vitro were also activated myofibroblasts with coexpression of vimentin, alpha-SMA (36.2 +/- 3.7%, 19.3 +/- 2.4%, and 60.3 +/- 9% positive cells, respectively), strong MMP-13 activity indicative of collagen remodeling, and SMemb ('remodeling/activated' phenotype: V+/A+/M+/S+). In contrast, VIC in long-term pulmonary autografts and engineered valve explants had a mostly fibroblast-like phenotype, with sparse alpha-SMA expression (6.0 +/- 1% and 5.4 +/- 1.0% positive cells) (V+/A-/M-/S-). CONCLUSION: Most VIC in normal valves were quiescent with a fibroblast-like phenotype. VIC in developing, diseased, adapting and engineered valves adjust to a dynamic environment through VIC activation and secretion of proteolytic enzymes mediating extracellular matrix remodeling ('developing/ remodeling/activated' phenotype), followed by a normalization of phenotype.
[Mh] Termos MeSH primário: Fibroblastos/fisiologia
Doenças das Valvas Cardíacas/fisiopatologia
Valvas Cardíacas/fisiologia
[Mh] Termos MeSH secundário: Actinas/biossíntese
Adulto
Animais
Colagenases/biossíntese
Desenvolvimento Embrionário e Fetal/fisiologia
Próteses Valvulares Cardíacas
Valvas Cardíacas/citologia
Valvas Cardíacas/embriologia
Valvas Cardíacas/fisiopatologia
Seres Humanos
Metaloproteinase 13 da Matriz
Músculo Liso/metabolismo
Miosinas/biossíntese
Fenótipo
Ovinos
Engenharia Tecidual
Vimentina/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Actins); 0 (Vimentin); EC 3.4.24.- (Collagenases); EC 3.4.24.- (MMP13 protein, human); EC 3.4.24.- (Matrix Metalloproteinase 13); EC 3.6.4.1 (Myosins)
[Em] Mês de entrada:0411
[Cu] Atualização por classe:091119
[Lr] Data última revisão:
091119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:041012
[St] Status:MEDLINE


  4 / 22621 MEDLINE  
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[PMID]:15469172
[Au] Autor:Kably Ambe A; Ruiz Anguas J; Carballo Mondragón E; Corona de Lau C; Karchmer Krivitsky S
[Ad] Endereço:Hospital Angeles de Las Lomas, Centro Especializado para la Atención de la Mujer, Unidad de Reproducción Asistida. cepam@infosel.com.mx
[Ti] Título:[Correlation between follicle levels of superoxide dismutase and oocyte quality, fertilization rates and embryo development].
[Ti] Título:Correlación entre las concentraciones intrafoliculares de la superóxido dismutasa y la calidad ovocitaria, las tasas de fertilización y el desarrollo embrionario..
[So] Source:Ginecol Obstet Mex;72:335-44, 2004 Jul.
[Is] ISSN:0300-9041
[Cp] País de publicação:Mexico
[La] Idioma:spa
[Ab] Resumo:BACKGROUND: The intraovarian oxidative balance is important during oocyte development, and fertilization. It has been proposed that one of the most important enzymes in the follicle is the superoxide dismutase (SOD). OBJECTIVE: To correlate levels and percentage of SOD activity in follicular liquid with quality, fertilization and embryo development in a group of patients submitted to in vitro fertilization. MATERIAL AND METHODS: We obtained 120 follicular liquids from oocytes aspirated in 41 patients during an IVF program and then we followed the development of each oocyte separately. We measured the activity and concentration of SOD in the follicular liquid, and we evaluated the following variables: quality and maturity in the oocytes, as well as fertilization rate, segmentation rate and pregnancy. The statistical analysis was made with ANOVA test and Pearson test. RESULTS: In the analysis of the results, we observed a higher percentage of activity in the SOD in oocytes with good quality (3 and 4) in comparison with poor quality oocyte (1 and 2) (89 and 82% vs 75 and 61% p<0.05). We observed higher concentrations and activity of SOD in oocytes with a good fertilization rate and segmentation (p<0.05). When we analyzed the variables in function of pregnancy, we observed that the embryos that were transferred and developed pregnancy had higher concentrations and activity of SOD than embryos that did not develop pregnancy. CONCLUSIONS: Elevated levels and high percentage in the activity of SOD are associated with a better quality in the oocyte, and a good embryo development, influenced by the oxidative balance.
[Mh] Termos MeSH primário: Desenvolvimento Embrionário e Fetal
Fertilização In Vitro
Oócitos/fisiologia
Folículo Ovariano/enzimologia
Superóxido Dismutase/metabolismo
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:0411
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:041008
[St] Status:MEDLINE


  5 / 22621 MEDLINE  
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[PMID]:15467528
[Au] Autor:Garite TJ; Clark RH; Elliott JP; Thorp JA
[Ad] Endereço:Department of Obstetrics and Gynecology, University of California, Irvine, Calif, USA. tjgarite@uci.edu
[Ti] Título:Twins and triplets: the effect of plurality and growth on neonatal outcome compared with singleton infants.
[So] Source:Am J Obstet Gynecol;191(3):700-7, 2004 Sep.
[Is] ISSN:0002-9378
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Information on outcome by gestational age from large numbers of twins and triplets is limited and is important for counseling and decision-making in obstetric practice. We reviewed one of the largest available neonatal databases to describe mortality and morbidity rates and growth in newborn infants from multiple gestations and compared these data with data for singletons. STUDY DESIGN: Data from a large prospectively recorded neonatal database that incorporated neonatal records from January 1997 to July 2002 were reviewed. We evaluated birth weight and neonatal mortality and morbidity rates that affected long-term outcome for each week of gestational age from 23 to 35 weeks of gestation for all nonanomolous inborn twins and triplets who were admitted to the neonatal intensive care unit and compared these data to all singletons who met similar criteria during the same time period. RESULTS: There were 12,302 twin and 2155 triplet births that met the entry criteria. The data for these newborn infants were compared with 36,931 singletons. Average birth weights at each gestational week were similar for all gestational ages until 29 weeks of gestation for triplets and 32 weeks of gestation for twins. After these gestational ages, the entire difference between twins and singletons was due to the weight of the smaller twin; the larger twins' mean weights were similar to singletons at all weeks that were studied. Birth order at each week also did not affect neonatal mortality rates, even when corrected for route of delivery and antenatal steroids. Neonatal morbidities associated with adverse long-term outcomes (intraventricular hemorrhage, retinopathy of prematurity, necrotizing enterocolitis) were also not different between multiple infants and singletons. Intrauterine growth restriction (IUGR) was associated with increased mortality rates at all gestational ages, but in the absences of IUGR, discordance was not. CONCLUSION: Data on a large number of twins and triplets provide reassurance that neonatal outcome at all viable premature weeks of gestation are similar to singletons. Intrauterine growth restriction and prematurity are therefore the principal issues that drive neonatal mortality and morbidity rates in multiple gestations. These data are important for obstetric decision-making and patient counseling.
[Mh] Termos MeSH primário: Desenvolvimento Embrionário e Fetal
Idade Gestacional
Resultado da Gravidez
Trigêmeos
Gêmeos
[Mh] Termos MeSH secundário: Peso ao Nascer
Feminino
Retardo do Crescimento Fetal/mortalidade
Seres Humanos
Mortalidade Infantil
Recém-Nascido
Recém-Nascido Prematuro
Terapia Intensiva Neonatal
Morbidade
Gravidez
Gravidez Múltipla
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:0411
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:041007
[St] Status:MEDLINE


  6 / 22621 MEDLINE  
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PubMed Central Texto completo
[PMID]:15466500
[Au] Autor:Oken E; Kleinman KP; Olsen SF; Rich-Edwards JW; Gillman MW
[Ad] Endereço:Department of Ambulatory Care and Prevention, Harvard Medical School and Harvard Pilgrim Health Care, Boston, MA, USA. emily_oken@harvardpilgrim.org
[Ti] Título:Associations of seafood and elongated n-3 fatty acid intake with fetal growth and length of gestation: results from a US pregnancy cohort.
[So] Source:Am J Epidemiol;160(8):774-83, 2004 Oct 15.
[Is] ISSN:0002-9262
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previous studies, mainly among populations with high consumption of seafood, have suggested that increased marine n-3 polyunsaturated fatty acid (PUFA) intake during pregnancy promotes longer gestation and higher birth weight. Few studies have isolated the contribution of fetal growth to birth weight. Using data from 2,109 pregnant women in Massachusetts enrolled in Project Viva from 1999 to 2002, the authors examined associations of marine n-3 PUFA and seafood intake with birth weight and birth-weight-for-gestational-age z value (fetal growth) using linear regression; length of gestation using median regression; and low birth weight, preterm delivery, and being small for gestational age using logistic regression. After adjustment for maternal and child factors, birth weight was 94 (95% confidence interval: 23, 166) g lower and fetal growth z value 0.19 (95% confidence interval: 0.08, 0.31) units lower in the highest compared with the lowest quartile of first-trimester n-3 PUFA intake. Results for the second and third trimesters were similar, and findings for seafood paralleled those for n-3 PUFA. Elongated n-3 PUFA intake and seafood intake were not associated with length of gestation or risk of preterm birth. Results from this US cohort support the conclusion that seafood intake during pregnancy is associated with reduced fetal growth.
[Mh] Termos MeSH primário: Peso ao Nascer
Desenvolvimento Embrionário e Fetal
Ácidos Graxos Ômega-3/administração & dosagem
Idade Gestacional
Alimentos Marinhos
[Mh] Termos MeSH secundário: Inquéritos sobre Dietas
Ingestão de Energia
Feminino
Retardo do Crescimento Fetal/epidemiologia
Retardo do Crescimento Fetal/etiologia
Retardo do Crescimento Fetal/prevenção & controle
Seres Humanos
Recém-Nascido de Baixo Peso
Recém-Nascido
Recém-Nascido Pequeno para a Idade Gestacional
Modelos Lineares
Modelos Logísticos
Masculino
Massachusetts/epidemiologia
Análise Multivariada
Obesidade/complicações
Obesidade/epidemiologia
Trabalho de Parto Prematuro/epidemiologia
Trabalho de Parto Prematuro/etiologia
Trabalho de Parto Prematuro/prevenção & controle
Gravidez
Complicações na Gravidez/epidemiologia
Resultado da Gravidez/epidemiologia
Estudos Prospectivos
Fatores de Risco
Fumar/efeitos adversos
Fumar/epidemiologia
Inquéritos e Questionários
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Fatty Acids, Omega-3)
[Em] Mês de entrada:0411
[Cu] Atualização por classe:161122
[Lr] Data última revisão:
161122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:041007
[St] Status:MEDLINE


  7 / 22621 MEDLINE  
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[PMID]:15454936
[Au] Autor:Cobb J; Duboule D
[Ti] Título:Tracing microRNA patterns in mice.
[So] Source:Nat Genet;36(10):1033-4, 2004 Oct.
[Is] ISSN:1061-4036
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: MicroRNAs/genética
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas
Animais
Desenvolvimento Embrionário e Fetal/genética
Regulação da Expressão Gênica no Desenvolvimento
Genes Homeobox
Genes Reporter
Óperon Lac
Camundongos
Camundongos Transgênicos
Família Multigênica
[Pt] Tipo de publicação:COMMENT; NEWS
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (MicroRNAs)
[Em] Mês de entrada:0411
[Cu] Atualização por classe:041117
[Lr] Data última revisão:
041117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:040930
[St] Status:MEDLINE


  8 / 22621 MEDLINE  
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[PMID]:15383103
[Au] Autor:Baschat AA
[Ad] Endereço:Department of Obstetrics, Gynaecology and Reproductive Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA.
[Ti] Título:Fetal responses to placental insufficiency: an update.
[So] Source:BJOG;111(10):1031-41, 2004 Oct.
[Is] ISSN:1470-0328
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Retardo do Crescimento Fetal/etiologia
Insuficiência Placentária/etiologia
[Mh] Termos MeSH secundário: Glicemia/metabolismo
Desenvolvimento Embrionário e Fetal
Feminino
Retardo do Crescimento Fetal/metabolismo
Feto/metabolismo
Doenças Hematológicas/etiologia
Seres Humanos
Placenta/irrigação sanguínea
Gravidez
Ultrassonografia Pré-Natal/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Blood Glucose)
[Em] Mês de entrada:0410
[Cu] Atualização por classe:051116
[Lr] Data última revisão:
051116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:040924
[St] Status:MEDLINE


  9 / 22621 MEDLINE  
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[PMID]:15375258
[Au] Autor:Gluckman PD; Hanson MA
[Ad] Endereço:Liggins Institute, University of Auckland and National Research Centre for Growth and Development, 2-6 Park Avenue, Grafton, Private Bag 92019, Auckland, New Zealand. pd.gluckman@auckland.ac.nz
[Ti] Título:Living with the past: evolution, development, and patterns of disease.
[So] Source:Science;305(5691):1733-6, 2004 Sep 17.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Epidemiological observations have led to the hypothesis that the risk of developing some chronic noncommunicable diseases in adulthood is influenced not only by genetic and adult life-style factors but also by environmental factors acting in early life. Research in evolutionary biology, developmental biology, and animal and human physiology provides support for this idea and suggests that environmental processes influencing the propensity to disease in adulthood operate during the periconceptual, fetal, and infant phases of life. This "developmental origins of health and disease" concept may have important biological, medical, and socioeconomic implications.
[Mh] Termos MeSH primário: Evolução Biológica
Doença Crônica
Suscetibilidade a Doenças
Doença/etiologia
Desenvolvimento Embrionário e Fetal
Meio Ambiente
[Mh] Termos MeSH secundário: Animais
Peso ao Nascer
Sinais (Psicologia)
Feminino
Seres Humanos
Recém-Nascido
Estilo de Vida
Fenômenos Fisiológicos da Nutrição
Gravidez
Efeitos Tardios da Exposição Pré-Natal
Fatores de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.; REVIEW
[Em] Mês de entrada:0409
[Cu] Atualização por classe:101118
[Lr] Data última revisão:
101118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:040918
[St] Status:MEDLINE


  10 / 22621 MEDLINE  
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[PMID]:15374701
[Au] Autor:Bedaiwy MA; Falcone T; Mohamed MS; Aleem AA; Sharma RK; Worley SE; Thornton J; Agarwal A
[Ad] Endereço:Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
[Ti] Título:Differential growth of human embryos in vitro: role of reactive oxygen species.
[So] Source:Fertil Steril;82(3):593-600, 2004 Sep.
[Is] ISSN:0015-0282
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To examine the relationship of early human embryonic development with the level of reactive oxygen species (ROS) in the culture media on the first day (day 1 ROS) after insemination. DESIGN: A prospective study. SETTING: Patients undergoing assisted reproduction in a teaching hospital. PATIENT(S): Patients undergoing conventional IVF (n = 104; 115 cycles) and intracytoplasmic sperm injection (ICSI) (n = 91; 96 cycles) were included. Both fertilization and early cultures were performed in human tubal fluid with 5% serum substitute supplement. INTERVENTION(S): Day 1 ROS levels in the central well (sample) and the outer well (control) of each embryo culture dish were measured after overnight incubation by chemiluminescence assay using luminol as the probe. MAIN OUTCOME MEASURE(S): Fertilization rate and embryo quality at day 3 and 5 were recorded for each cycle. Age, parity, and demographic features were also compared. RESULT(S): High day 1 ROS levels in culture media were associated with low blastocyst rate, low fertilization rate, low cleavage rate, and high embryonic fragmentation with ICSI but not with conventional IVF. High day 1 ROS levels in culture media were associated with lower pregnancy rates in both IVF and ICSI cycles. CONCLUSION(S): Reactive oxygen species generated in culture media by day 1 may be an important biochemical marker for early embryonic growth. Increased embryonic fragmentation and slow cleavage rate may be partially attributed to early exposure of embryos to high ROS levels in ICSI cycles. Differential growth of ICSI embryos incubated under identical conditions may be in part due to differences in ROS levels of the culture medium surrounding these embryos.
[Mh] Termos MeSH primário: Desenvolvimento Embrionário e Fetal/fisiologia
Oócitos/citologia
Espécies Reativas de Oxigênio
Injeções de Esperma Intracitoplásmicas
Espermatozoides/citologia
[Mh] Termos MeSH secundário: Blastocisto/citologia
Implantação do Embrião
Feminino
Fertilização
Fertilização In Vitro
Seres Humanos
Técnicas In Vitro
Infertilidade Feminina/etiologia
Masculino
Indução da Ovulação/métodos
Gravidez
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Reactive Oxygen Species)
[Em] Mês de entrada:0411
[Cu] Atualização por classe:141120
[Lr] Data última revisão:
141120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:040918
[St] Status:MEDLINE



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