Base de dados : MEDLINE
Pesquisa : G07.345.500.325.180.812 [Categoria DeCS]
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[PMID]:29346450
[Au] Autor:Sridharan J; Haremaki T; Weinstein DC
[Ad] Endereço:Biology Department, Queens College of the City University of New York, Flushing, New York, United States of America.
[Ti] Título:Cloning and spatiotemporal expression of Xenopus laevis Apolipoprotein CI.
[So] Source:PLoS One;13(1):e0191470, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Apolipoprotein CI (ApoCI) belongs to the Apolipoprotein superfamily, members of which are involved in lipid transport, uptake and homeostasis. Excessive ApoCI has been implicated in atherosclerosis and Alzheimer's disease in humans. In this study we report the isolation of Xenopus laevis apoCI and describe the expression pattern of this gene during early development, using reverse transcription polymerase chain reaction and whole mount in situ hybridization. Xenopus apoCI is enriched in the dorsal ectoderm during gastrulation, and is subsequently expressed in sensory placodes, neural tube and cranial neural crest. These data suggest as yet uncharacterized roles for ApoCI during early vertebrate embryogenesis.
[Mh] Termos MeSH primário: Apolipoproteína C-I/genética
[Mh] Termos MeSH secundário: Animais
Clonagem Molecular
Gastrulação
Hibridização In Situ
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Xenopus laevis/embriologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Apolipoprotein C-I)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191470


  2 / 749 MEDLINE  
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[PMID]:29049289
[Au] Autor:Plouhinec JL; Medina-Ruiz S; Borday C; Bernard E; Vert JP; Eisen MB; Harland RM; Monsoro-Burq AH
[Ad] Endereço:Université Paris Sud, Université Paris Saclay, CNRS UMR 3347, INSERM U1021, Orsay, France.
[Ti] Título:A molecular atlas of the developing ectoderm defines neural, neural crest, placode, and nonneural progenitor identity in vertebrates.
[So] Source:PLoS Biol;15(10):e2004045, 2017 Oct.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During vertebrate neurulation, the embryonic ectoderm is patterned into lineage progenitors for neural plate, neural crest, placodes and epidermis. Here, we use Xenopus laevis embryos to analyze the spatial and temporal transcriptome of distinct ectodermal domains in the course of neurulation, during the establishment of cell lineages. In order to define the transcriptome of small groups of cells from a single germ layer and to retain spatial information, dorsal and ventral ectoderm was subdivided along the anterior-posterior and medial-lateral axes by microdissections. Principal component analysis on the transcriptomes of these ectoderm fragments primarily identifies embryonic axes and temporal dynamics. This provides a genetic code to define positional information of any ectoderm sample along the anterior-posterior and dorsal-ventral axes directly from its transcriptome. In parallel, we use nonnegative matrix factorization to predict enhanced gene expression maps onto early and mid-neurula embryos, and specific signatures for each ectoderm area. The clustering of spatial and temporal datasets allowed detection of multiple biologically relevant groups (e.g., Wnt signaling, neural crest development, sensory placode specification, ciliogenesis, germ layer specification). We provide an interactive network interface, EctoMap, for exploring synexpression relationships among genes expressed in the neurula, and suggest several strategies to use this comprehensive dataset to address questions in developmental biology as well as stem cell or cancer research.
[Mh] Termos MeSH primário: Ectoderma/embriologia
Crista Neural/embriologia
Neurônios/citologia
Células-Tronco/metabolismo
Xenopus laevis/embriologia
[Mh] Termos MeSH secundário: Algoritmos
Animais
Análise por Conglomerados
Bases de Dados Genéticas
Ectoderma/metabolismo
Gastrulação/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica no Desenvolvimento
Ontologia Genética
Redes Reguladoras de Genes
Seres Humanos
Internet
Microdissecção
Neoplasias/genética
Crista Neural/metabolismo
Neurulação/genética
Análise de Componente Principal
Fatores de Tempo
Transcriptoma/genética
Proteínas Wnt/metabolismo
Xenopus laevis/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Wnt Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171020
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.2004045


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[PMID]:29033362
[Au] Autor:Barone V; Lang M; Krens SFG; Pradhan SJ; Shamipour S; Sako K; Sikora M; Guet CC; Heisenberg CP
[Ad] Endereço:Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
[Ti] Título:An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate.
[So] Source:Dev Cell;43(2):198-211.e12, 2017 Oct 23.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cell-cell contact formation constitutes an essential step in evolution, leading to the differentiation of specialized cell types. However, remarkably little is known about whether and how the interplay between contact formation and fate specification affects development. Here, we identify a positive feedback loop between cell-cell contact duration, morphogen signaling, and mesendoderm cell-fate specification during zebrafish gastrulation. We show that long-lasting cell-cell contacts enhance the competence of prechordal plate (ppl) progenitor cells to respond to Nodal signaling, required for ppl cell-fate specification. We further show that Nodal signaling promotes ppl cell-cell contact duration, generating a positive feedback loop between ppl cell-cell contact duration and cell-fate specification. Finally, by combining mathematical modeling and experimentation, we show that this feedback determines whether anterior axial mesendoderm cells become ppl or, instead, turn into endoderm. Thus, the interdependent activities of cell-cell signaling and contact formation control fate diversification within the developing embryo.
[Mh] Termos MeSH primário: Comunicação Celular
Linhagem da Célula
Retroalimentação Fisiológica
Gástrula/metabolismo
Morfogênese/fisiologia
Proteínas de Peixe-Zebra/metabolismo
Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Animais
Padronização Corporal
Diferenciação Celular
Embrião não Mamífero/citologia
Embrião não Mamífero/metabolismo
Desenvolvimento Embrionário
Gástrula/crescimento & desenvolvimento
Gastrulação/fisiologia
Regulação da Expressão Gênica no Desenvolvimento
Modelos Teóricos
Proteína Nodal/genética
Proteína Nodal/metabolismo
Transdução de Sinais
Células-Tronco/citologia
Células-Tronco/metabolismo
Peixe-Zebra/embriologia
Proteínas de Peixe-Zebra/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nodal Protein); 0 (Zebrafish Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE


  4 / 749 MEDLINE  
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[PMID]:28923486
[Au] Autor:Nikaido M; Acedo JN; Hatta K; Piotrowski T
[Ad] Endereço:Stowers Institute for Medical Research, Kansas City, MO 64110, USA; Graduate School of Life Sciences, University of Hyogo, Hyogo Pref. 678-1297, Japan.
[Ti] Título:Retinoic acid is required and Fgf, Wnt, and Bmp signaling inhibit posterior lateral line placode induction in zebrafish.
[So] Source:Dev Biol;431(2):215-225, 2017 11 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The lateral line system is a mechanosensory systems present in aquatic animals. The anterior and posterior lateral lines develop from anterior and posterior lateral line placodes (aLLp and pLLp), respectively. Although signaling molecules required for the induction of other cranial placodes have been well studied, the molecular mechanisms underlying formation of the lateral line placodes are unknown. In this study we tested the requirement of multiple signaling pathways, such as Wnt, Bmp Fgf, and Retinoic Acid for aLLp and pLLp induction. We determined that aLLp specification requires Fgf signaling, whilst pLLp specification requires retinoic acid which inhibits Fgf signaling. pLLp induction is also independent of Wnt and Bmp activities, even though these pathways limit the boundaries of the pLLp. This is the first report that the aLLp and pLLp depend on different inductive mechanisms and that pLLp induction requires the inhibition of Fgf, Wnt and Bmp signaling.
[Mh] Termos MeSH primário: Proteínas Morfogenéticas Ósseas/metabolismo
Fatores de Crescimento de Fibroblastos/metabolismo
Sistema da Linha Lateral/embriologia
Transdução de Sinais
Tretinoína/farmacologia
Proteínas Wnt/metabolismo
Peixe-Zebra/embriologia
Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Animais
Benzaldeídos/farmacologia
Padronização Corporal/efeitos dos fármacos
Padronização Corporal/genética
Gastrulação/efeitos dos fármacos
Sistema da Linha Lateral/efeitos dos fármacos
Sistema da Linha Lateral/metabolismo
Transdução de Sinais/efeitos dos fármacos
Proteínas de Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzaldehydes); 0 (Bone Morphogenetic Proteins); 0 (Wnt Proteins); 0 (Zebrafish Proteins); 5688UTC01R (Tretinoin); 62031-54-3 (Fibroblast Growth Factors)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


  5 / 749 MEDLINE  
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[PMID]:28919206
[Au] Autor:Díaz-Díaz C; Fernandez de Manuel L; Jimenez-Carretero D; Montoya MC; Clavería C; Torres M
[Ad] Endereço:Cardiovascular Development Program, Centro Nacional de Investigaciones Cardiovasculares, CNIC, Madrid 28029, Spain.
[Ti] Título:Pluripotency Surveillance by Myc-Driven Competitive Elimination of Differentiating Cells.
[So] Source:Dev Cell;42(6):585-599.e4, 2017 Sep 25.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mammalian epiblast is formed by pluripotent cells able to differentiate into all tissues of the new individual. In their progression to differentiation, epiblast cells and their in vitro counterparts, embryonic stem cells (ESCs), transit from naive pluripotency through a differentiation-primed pluripotent state. During these events, epiblast cells and ESCs are prone to death, driven by competition between Myc-high cells (winners) and Myc-low cells (losers). Using live tracking of Myc levels, we show that Myc-high ESCs approach the naive pluripotency state, whereas Myc-low ESCs are closer to the differentiation-primed state. In ESC colonies, naive cells eliminate differentiating cells by cell competition, which is determined by a limitation in the time losers are able to survive persistent contact with winners. In the mouse embryo, cell competition promotes pluripotency maintenance by elimination of primed lineages before gastrulation. The mechanism described here is relevant to mammalian embryo development and induced pluripotency.
[Mh] Termos MeSH primário: Diferenciação Celular
Células-Tronco Pluripotentes/citologia
Células-Tronco Pluripotentes/metabolismo
Proteínas Proto-Oncogênicas c-myc/metabolismo
[Mh] Termos MeSH secundário: Animais
Comunicação Celular
Linhagem da Célula
Proliferação Celular
Sobrevivência Celular
Rastreamento de Células
Células Cultivadas
Embrião de Mamíferos/citologia
Gastrulação
Perfilação da Expressão Gênica
Camadas Germinativas/citologia
Padrões de Herança/genética
Camundongos
Células-Tronco Embrionárias Murinas/citologia
Células-Tronco Embrionárias Murinas/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proto-Oncogene Proteins c-myc)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE


  6 / 749 MEDLINE  
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[PMID]:28818668
[Au] Autor:Amiel AR; Johnston H; Chock T; Dahlin P; Iglesias M; Layden M; Röttinger E; Martindale MQ
[Ad] Endereço:Université Côte d'Azur, CNRS, INSERM, Institute for Research on Cancer and Aging, Nice, France.
[Ti] Título:A bipolar role of the transcription factor ERG for cnidarian germ layer formation and apical domain patterning.
[So] Source:Dev Biol;430(2):346-361, 2017 10 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Germ layer formation and axial patterning are biological processes that are tightly linked during embryonic development of most metazoans. In addition to canonical WNT, it has been proposed that ERK-MAPK signaling is involved in specifying oral as well as aboral territories in cnidarians. However, the effector and the molecular mechanism underlying latter phenomenon is unknown. By screening for potential effectors of ERK-MAPK signaling in both domains, we identified a member of the ETS family of transcription factors, Nverg that is bi-polarily expressed prior to gastrulation. We further describe the crucial role of NvERG for gastrulation, endomesoderm as well as apical domain formation. The molecular characterization of the obtained NvERG knock-down phenotype using previously described as well as novel potential downstream targets, provides evidence that a single transcription factor, NvERG, simultaneously controls expression of two different sets of downstream targets, leading to two different embryonic gene regulatory networks (GRNs) in opposite poles of the developing embryo. We also highlight the molecular interaction of cWNT and MEK/ERK/ERG signaling that provides novel insight into the embryonic axial organization of Nematostella, and show a cWNT repressive role of MEK/ERK/ERG signaling in segregating the endomesoderm in two sub-domains, while a common input of both pathways is required for proper apical domain formation. Taking together, we build the first blueprint for a global cnidarian embryonic GRN that is the foundation for additional gene specific studies addressing the evolution of embryonic and larval development.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica no Desenvolvimento
Camadas Germinativas/crescimento & desenvolvimento
Anêmonas-do-Mar/genética
Fatores de Transcrição/fisiologia
[Mh] Termos MeSH secundário: Animais
Padronização Corporal
DNA Complementar/genética
Embrião não Mamífero/metabolismo
Embrião não Mamífero/ultraestrutura
Fatores de Crescimento de Fibroblastos/fisiologia
Gastrulação/genética
Técnicas de Silenciamento de Genes
Redes Reguladoras de Genes
Camadas Germinativas/metabolismo
Sistema de Sinalização das MAP Quinases
Mesoderma/metabolismo
Anêmonas-do-Mar/embriologia
Anêmonas-do-Mar/ultraestrutura
Via de Sinalização Wnt
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Transcription Factors); 62031-54-3 (Fibroblast Growth Factors)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE


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[PMID]:28668387
[Au] Autor:Li YL; Shao M; Shi DL
[Ad] Endereço:School of Life Sciences, Shandong University, 27, Shanda Nan Road, Jinan 250100, China.
[Ti] Título:Rac1 signalling coordinates epiboly movement by differential regulation of actin cytoskeleton in zebrafish.
[So] Source:Biochem Biophys Res Commun;490(3):1059-1065, 2017 Aug 26.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dynamic cytoskeleton organization is essential for polarized cell behaviours in a wide variety of morphogenetic events. In zebrafish, epiboly involves coordinated cell shape changes and expansion of cell layers to close the blastopore, but many important regulatory aspects are still unclear. Especially, the spatio-temporal regulation and function of actin structures remain to be determined for a better understanding of the mechanisms that coordinate epiboly movement. Here we show that Rac1 signalling, likely functions downstream of phosphatiditylinositol-3 kinase, is required for F-actin organization during epiboly progression in zebtafish. Using a dominant negative mutant of Rac1 and specific inhibitors to block the activation of this pathway, we find that marginal contractile actin ring is sensitive to inhibition of Rac1 signalling. In particular, we identify a novel function for this actin structure in retaining the external yolk syncytial nuclei within the margin of enveloping layer for coordinated movement toward the vegetal pole. Furthermore, we find that F-actin bundles, progressively formed in the vegetal cortex of the yolk cell, act in concert with marginal actin ring and play an active role in pulling external yolk syncytial nuclei toward the vegetal pole direction. This study uncovers novel roles of different actin structures in orchestrating epiboly movement. It helps to provide insight into the mechanisms regulating cellular polarization during early development.
[Mh] Termos MeSH primário: Citoesqueleto de Actina/metabolismo
Transdução de Sinais
Proteínas de Peixe-Zebra/metabolismo
Peixe-Zebra/embriologia
Peixe-Zebra/metabolismo
Proteínas rac1 de Ligação ao GTP/metabolismo
[Mh] Termos MeSH secundário: Citoesqueleto de Actina/ultraestrutura
Animais
Forma Celular
Embrião não Mamífero/citologia
Embrião não Mamífero/embriologia
Embrião não Mamífero/metabolismo
Embrião não Mamífero/ultraestrutura
Gástrula/citologia
Gástrula/embriologia
Gástrula/metabolismo
Gástrula/ultraestrutura
Gastrulação
Fosfatidilinositol 3-Quinases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Zebrafish Proteins); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 3.6.5.2 (rac1 GTP-Binding Protein)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170703
[St] Status:MEDLINE


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[PMID]:28607482
[Au] Autor:Kobayashi T; Zhang H; Tang WWC; Irie N; Withey S; Klisch D; Sybirna A; Dietmann S; Contreras DA; Webb R; Allegrucci C; Alberio R; Surani MA
[Ad] Endereço:Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.
[Ti] Título:Principles of early human development and germ cell program from conserved model systems.
[So] Source:Nature;546(7658):416-420, 2017 06 15.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Human primordial germ cells (hPGCs), the precursors of sperm and eggs, originate during weeks 2-3 of early post-implantation development. Using in vitro models of hPGC induction, recent studies have suggested that there are marked mechanistic differences in the specification of human and mouse PGCs. This may be due in part to the divergence in their pluripotency networks and early post-implantation development. As early human embryos are not accessible for direct study, we considered alternatives including porcine embryos that, as in humans, develop as bilaminar embryonic discs. Here we show that porcine PGCs originate from the posterior pre-primitive-streak competent epiblast by sequential upregulation of SOX17 and BLIMP1 in response to WNT and BMP signalling. We use this model together with human and monkey in vitro models simulating peri-gastrulation development to show the conserved principles of epiblast development for competency for primordial germ cell fate. This process is followed by initiation of the epigenetic program and regulated by a balanced SOX17-BLIMP1 gene dosage. Our combinatorial approach using human, porcine and monkey in vivo and in vitro models provides synthetic insights into early human development.
[Mh] Termos MeSH primário: Diferenciação Celular
Desenvolvimento Embrionário
Células Germinativas/citologia
Macaca fascicularis/embriologia
Modelos Biológicos
Células-Tronco Pluripotentes/citologia
Suínos/embriologia
[Mh] Termos MeSH secundário: Animais
Proteínas Morfogenéticas Ósseas/metabolismo
Linhagem da Célula
Corpos Embrioides/citologia
Epigênese Genética
Feminino
Gastrulação
Dosagem de Genes
Células Germinativas/metabolismo
Camadas Germinativas/citologia
Seres Humanos
Técnicas In Vitro
Masculino
Modelos Animais
Fator 1 de Ligação ao Domínio I Regulador Positivo
Linha Primitiva/citologia
Proteínas Repressoras/genética
Fatores de Transcrição SOXF/genética
Via de Sinalização Wnt
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bone Morphogenetic Proteins); 0 (Repressor Proteins); 0 (SOX17 protein, human); 0 (SOXF Transcription Factors); 138415-26-6 (PRDM1 protein, human); EC 2.1.1.- (Positive Regulatory Domain I-Binding Factor 1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1038/nature22812


  9 / 749 MEDLINE  
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[PMID]:28602952
[Au] Autor:Abdol AM; Röttinger E; Jansson F; Kaandorp JA
[Ad] Endereço:Computational Science Lab, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. Electronic address: a.m.abdol@uva.nl.
[Ti] Título:A novel technique to combine and analyse spatial and temporal expression datasets: A case study with the sea anemone Nematostella vectensis to identify potential gene interactions.
[So] Source:Dev Biol;428(1):204-214, 2017 08 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Understanding genetic interactions during early development of a given organism, is the first step toward unveiling gene regulatory networks (GRNs) that govern a biological process of interest. Predicting such interactions from large expression datasets by performing targeted knock-down/knock-out approaches is a challenging task. We use the currently available expression datasets (in situ hybridization images & qPCR time series) for a basal anthozoan the sea anemone N. vectensis to construct continuous spatiotemporal gene expression patterns during its early development. Moreover, by combining cluster results from each dataset we develop a method that provides testable hypotheses about potential genetic interactions. We show that the analysis of spatial gene expression patterns reveals functional regions of the embryo during the gastrulation. The clustering results from qPCR time series unveils significant temporal events and highlights genes potentially involved in N. vectensis gastrulation. Furthermore, we introduce a method for merging the clustering results from spatial and temporal datasets by which we can group genes that are expressed in the same region and at the time. We demonstrate that the merged clusters can be used to identify GRN interactions involved in various processes and to predict possible activators or repressors of any gene in the dataset. Finally, we validate our methods and results by predicting the repressor effect of NvErg on NvBra in the central domain during the gastrulation that has recently been confirmed by functional analysis.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica no Desenvolvimento/genética
Redes Reguladoras de Genes/genética
Anêmonas-do-Mar/embriologia
Anêmonas-do-Mar/genética
[Mh] Termos MeSH secundário: Animais
Análise por Conglomerados
Gastrulação/genética
Perfilação da Expressão Gênica
Análise Espaço-Temporal
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE


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[PMID]:28512197
[Au] Autor:Krens SFG; Veldhuis JH; Barone V; Capek D; Maître JL; Brodland GW; Heisenberg CP
[Ad] Endereço:Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
[Ti] Título:Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation.
[So] Source:Development;144(10):1798-1806, 2017 05 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences in cell adhesion and cortex tension are commonly thought to drive cell sorting by regulating tissue surface tension (TST). However, the role that differential TST plays in cell segregation within the developing embryo is as yet unclear. Here, we have analyzed the role of differential TST for germ layer progenitor cell segregation during zebrafish gastrulation. Contrary to previous observations that differential TST drives germ layer progenitor cell segregation , we show that germ layers display indistinguishable TST within the gastrulating embryo, arguing against differential TST driving germ layer progenitor cell segregation We further show that the osmolarity of the interstitial fluid (IF) is an important factor that influences germ layer TST , and that lower osmolarity of the IF compared with standard cell culture medium can explain why germ layers display differential TST in culture but not Finally, we show that directed migration of mesendoderm progenitors is required for germ layer progenitor cell segregation and germ layer formation.
[Mh] Termos MeSH primário: Padronização Corporal
Movimento Celular
Líquido Extracelular/química
Gastrulação/fisiologia
Células-Tronco/química
Células-Tronco/fisiologia
Peixe-Zebra/embriologia
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Embrião não Mamífero
Mesoderma/química
Mesoderma/citologia
Mesoderma/embriologia
Concentração Osmolar
Células-Tronco/citologia
Tensão Superficial
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170518
[St] Status:MEDLINE
[do] DOI:10.1242/dev.144964



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