Base de dados : MEDLINE
Pesquisa : G07.345.500.325.377.765 [Categoria DeCS]
Referências encontradas : 63 [refinar]
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[PMID]:28578021
[Au] Autor:Mohanty JN; Nayak S; Jha S; Joshi RK
[Ad] Endereço:Functional Genomics Laboratory, Centre of Biotechnology, Siksha O Anusandhan University, Kalinga Nagar, Ghatikia, Bhubaneswar 751003, India.
[Ti] Título:Transcriptome profiling of the floral buds and discovery of genes related to sex-differentiation in the dioecious cucurbit Coccinia grandis (L.) Voigt.
[So] Source:Gene;626:395-406, 2017 Aug 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Dioecious species offer an inclusive structure to study the molecular basis of sexual dimorphism in angiosperms. Despite having a small genome and heteromorphic sex chromosomes, Coccinia grandis is a highly neglected dioecious species with little information available on its physical state, genetic orientation and key sex-defining elements. In the present study, we performed RNA-Seq and DGE analysis of male (MB) and female (FB) buds in C. grandis to gain insights into the molecular basis of sex determination in this plant. De novo assembly of 75 million clean reads resulted in 72,479 unigenes for male library and 63,308 unigenes for female library with a mean length of 736bp. 61,458 (85.57%) unigenes displayed significant similarity with protein sequences from publicly available databases. Comparative transcriptome analyses revealed 1410 unigenes as differentially expressed (DEGs) between MB and FB samples. A consistent correlation between the expression levels of DEGs was observed for the RNA-Seq pattern and qRT-PCR validation. Functional annotation showed high enrichment of DEGs involved in phytohormone biosynthesis, hormone signaling and transduction, transcriptional regulation and methyltransferase activity. High induction of hormone responsive genes such as ARF6, ACC synthase1, SNRK2 and BRI1-associated receptor kinase 1 (BAK1) suggest that multiple phytohormones and their signaling crosstalk play crucial role in sex determination in this species. Beside, the transcription factors such as zinc fingers, homeodomain leucine zippers and MYBs were identified as major determinants of male specific expression. Moreover, the detection of multiple DEGs as the miRNA target site implies that a small RNA mediated gene silencing cascade may also be regulating gender differentiation in C. grandis. Overall, the present transcriptome resources provide us a large number of DEGs involved in sex expression and could form the groundwork for unravelling the molecular mechanism of sex determination in C. grandis.
[Mh] Termos MeSH primário: Cucurbitaceae/genética
Flores/genética
Regulação da Expressão Gênica no Desenvolvimento
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Transcriptoma
[Mh] Termos MeSH secundário: Cromossomos de Plantas/genética
Cucurbitaceae/crescimento & desenvolvimento
Flores/crescimento & desenvolvimento
MicroRNAs/genética
Organogênese Vegetal/genética
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Cromossomos Sexuais/genética
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (Plant Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170605
[St] Status:MEDLINE


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[PMID]:27737904
[Au] Autor:Cucinotta M; Manrique S; Guazzotti A; Quadrelli NE; Mendes MA; Benkova E; Colombo L
[Ad] Endereço:Dipartimento di BioScienze, Università degli studi di Milano, Via Celoria 26, Milano 20133, Italy.
[Ti] Título:Cytokinin response factors integrate auxin and cytokinin pathways for female reproductive organ development.
[So] Source:Development;143(23):4419-4424, 2016 12 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The developmental programme of the pistil is under the control of both auxin and cytokinin. Crosstalk between these factors converges on regulation of the auxin carrier PIN-FORMED 1 (PIN1). Here, we show that in the triple transcription factor mutant cytokinin response factor 2 (crf2) crf3 crf6 both pistil length and ovule number were reduced. PIN1 expression was also lower in the triple mutant and the phenotypes could not be rescued by exogenous cytokinin application. pin1 complementation studies using genomic PIN1 constructs showed that the pistil phenotypes were only rescued when the PCRE1 domain, to which CRFs bind, was present. Without this domain, pin mutants resemble the crf2 crf3 crf6 triple mutant, indicating the pivotal role of CRFs in auxin-cytokinin crosstalk.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Flores/embriologia
Proteínas de Membrana Transportadoras/metabolismo
Óvulo Vegetal/embriologia
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Arabidopsis/embriologia
Arabidopsis/genética
Proteínas de Arabidopsis/biossíntese
Flores/genética
Organogênese Vegetal/genética
Óvulo Vegetal/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (CRF2 protein, Arabidopsis); 0 (CRF3 protein, Arabidopsis); 0 (CRF6 protein, Arabidopsis); 0 (Membrane Transport Proteins); 0 (PCRE1 protein, Arabidopsis); 0 (PIN1 protein, Arabidopsis); 0 (Transcription Factors)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161015
[St] Status:MEDLINE


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[PMID]:27717460
[Au] Autor:Johnsson C; Fischer U
[Ad] Endereço:Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 901 83 Umeå, Sweden. Electronic address: Christoffer.Johnsson@slu.se.
[Ti] Título:Cambial stem cells and their niche.
[So] Source:Plant Sci;252:239-245, 2016 Nov.
[Is] ISSN:1873-2259
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Unlike animals, plants often have an indefinite genetic potency to form new organs throughout their entire lifespan. Growth and organogenesis are driven by cell divisions in meristems at distinct sites within the plant. Since the meristems contributing to axial thickening in dicots (cambia) are separated from places where axes elongate (apical meristems); there is a need of communication to coordinate growth. In their behavior, some meristematic cells resemble animal stem cells whose daughter cells either maintain the capacity to divide over a long period of time or undergo differentiation. The behavior of stem cells is regulated by their microenvironment, the so called niche. The stem- and niche-cell concept is now also widely accepted for apical meristems. An integral part of the cambial niche has recently been localized to the phloem. It steers cell division activity in the cambium via the release of a peptide signal and may be a hub to integrate signals from other stem cell populations to coordinate growth. Although these signals have yet to be determined, the discovery of the cambial niche cells will pave the way for a better understanding of inter-meristematic communication and cambial stem cell behavior.
[Mh] Termos MeSH primário: Meristema/fisiologia
Organogênese Vegetal
Desenvolvimento Vegetal
[Mh] Termos MeSH secundário: Comunicação Celular
Meristema/metabolismo
Transdução de Sinais
Nicho de Células-Tronco
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161009
[St] Status:MEDLINE


  4 / 63 MEDLINE  
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[PMID]:27287881
[Au] Autor:Buendía-Monreal M; Gillmor CS
[Ad] Endereço:Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), Unidad de Genómica Avanzada, CINVESTAV-IPN, Irapuato, Guanajuato, Mexico.
[Ti] Título:Mediator: A key regulator of plant development.
[So] Source:Dev Biol;419(1):7-18, 2016 11 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mediator is a multiprotein complex that regulates transcription at the level of RNA pol II assembly, as well as through regulation of chromatin architecture, RNA processing and recruitment of epigenetic marks. Though its modular structure is conserved in eukaryotes, its subunit composition has diverged during evolution and varies in response to environmental and tissue-specific inputs, suggesting different functions for each subunit and/or Mediator conformation. In animals, Mediator has been implicated in the control of differentiation and morphogenesis through modulation of numerous signaling pathways. In plants, studies have revealed roles for Mediator in regulation of cell division, cell fate and organogenesis, as well as developmental timing and hormone responses. We begin this review with an overview of biochemical mechanisms of yeast and animal Mediator that are likely to be conserved in all eukaryotes, as well as a brief discussion of the role of Mediator in animal development. We then present a comprehensive review of studies of the role of Mediator in plant development. Finally, we point to important questions for future research on the role of Mediator as a master coordinator of development.
[Mh] Termos MeSH primário: Complexo Mediador/fisiologia
Desenvolvimento Vegetal/fisiologia
Proteínas de Plantas/fisiologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Arabidopsis/fisiologia
Células Eucarióticas/metabolismo
Previsões
Proteínas Fúngicas/fisiologia
Regulação da Expressão Gênica de Plantas
Seres Humanos
Organogênese Vegetal/fisiologia
Reguladores de Crescimento de Planta/fisiologia
RNA Polimerase II/metabolismo
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Fungal Proteins); 0 (Mediator Complex); 0 (Plant Growth Regulators); 0 (Plant Proteins); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160612
[St] Status:MEDLINE


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[PMID]:27285948
[Au] Autor:Zhang J; Gai M; Li X; Li T; Sun H
[Ad] Endereço:a Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province , College of Horticulture, Shenyang Agricultural University , Shenyang , PR China.
[Ti] Título:Somatic embryogenesis and direct as well as indirect organogenesis in Lilium pumilum DC. Fisch., an endangered ornamental and medicinal plant.
[So] Source:Biosci Biotechnol Biochem;80(10):1898-906, 2016 Oct.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Somatic embryogenesis and organogenesis in Lilium pumilum were successfully regulated by picloram, α-naphthaleneacetic acid (NAA), and 6-benzyladenine (BA). In organogenesis, the highest shoot regeneration frequency (92.5%) was obtained directly from bulb scales on Murashige and Skoog (MS) medium containing 2.0 mg L(-1) BA and 0.2 mg L(-1) NAA, while organogenic callus (OC) formed from leaves on MS medium supplemented with 1.0 mg L(-1) BA and 0.5 mg L(-1) NAA. Following subculture, 76.7% of OC regenerated shoots. In somatic embryogenesis, the combination of picloram and NAA increased the amount of embryogenic callus (EC) that formed with a maximum on 90.7% of all explants which formed 11 somatic embryos (SEs) per explant. Differences between EC and OC in cellular morphology and cell differentiation fate were easily observed. SEs initially formed via an exogenous or an endogenous origin. The appearance of a protoderm in heart-shaped SE and the bipolar shoot-root development in oval-shaped SE indicated true somatic embryogenesis. This protocol provides a new and detailed regulation and histological examination of regeneration pattern in L. pumilum.
[Mh] Termos MeSH primário: Espécies em Perigo de Extinção
Lilium/fisiologia
Organogênese Vegetal
Sementes/fisiologia
[Mh] Termos MeSH secundário: Compostos de Benzil/farmacologia
Lilium/efeitos dos fármacos
Lilium/metabolismo
Ácidos Naftalenoacéticos/farmacologia
Organogênese Vegetal/efeitos dos fármacos
Picloram/farmacologia
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/crescimento & desenvolvimento
Folhas de Planta/metabolismo
Plantas Medicinais
Purinas/farmacologia
Regeneração/efeitos dos fármacos
Sementes/efeitos dos fármacos
Sementes/metabolismo
Amido/metabolismo
Sacarose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzyl Compounds); 0 (Naphthaleneacetic Acids); 0 (Purines); 57-50-1 (Sucrose); 9005-25-8 (Starch); KXG6A989PS (benzylaminopurine); O7437X49DW (Picloram)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160611
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2016.1194178


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[PMID]:27143753
[Au] Autor:Ikeuchi M; Ogawa Y; Iwase A; Sugimoto K
[Ad] Endereço:RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa 230-0045, Japan.
[Ti] Título:Plant regeneration: cellular origins and molecular mechanisms.
[So] Source:Development;143(9):1442-51, 2016 05 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Compared with animals, plants generally possess a high degree of developmental plasticity and display various types of tissue or organ regeneration. This regenerative capacity can be enhanced by exogenously supplied plant hormones in vitro, wherein the balance between auxin and cytokinin determines the developmental fate of regenerating organs. Accumulating evidence suggests that some forms of plant regeneration involve reprogramming of differentiated somatic cells, whereas others are induced through the activation of relatively undifferentiated cells in somatic tissues. We summarize the current understanding of how plants control various types of regeneration and discuss how developmental and environmental constraints influence these regulatory mechanisms.
[Mh] Termos MeSH primário: Arabidopsis/crescimento & desenvolvimento
Organogênese Vegetal/fisiologia
Reguladores de Crescimento de Planta/metabolismo
Raízes de Plantas/crescimento & desenvolvimento
Brotos de Planta/crescimento & desenvolvimento
Regeneração/fisiologia
[Mh] Termos MeSH secundário: Arabidopsis/fisiologia
Reprogramação Celular/fisiologia
Citocininas/metabolismo
Ácidos Indolacéticos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokinins); 0 (Indoleacetic Acids); 0 (Plant Growth Regulators)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160505
[St] Status:MEDLINE
[do] DOI:10.1242/dev.134668


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[PMID]:27048938
[Au] Autor:Wang Z; Li N; Jiang S; Gonzalez N; Huang X; Wang Y; Inzé D; Li Y
[Ad] Endereço:State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Center for Excellence in Molecular Plant Sciences, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Lincui East Road, Chaoyang District, Beijing 100101, China.
[Ti] Título:SCF(SAP) controls organ size by targeting PPD proteins for degradation in Arabidopsis thaliana.
[So] Source:Nat Commun;7:11192, 2016 Apr 06.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Control of organ size by cell proliferation and growth is a fundamental process, but the mechanisms that determine the final size of organs are largely elusive in plants. We have previously revealed that the ubiquitin receptor DA1 regulates organ size by repressing cell proliferation in Arabidopsis. Here we report that a mutant allele of STERILE APETALA (SAP) suppresses the da1-1 mutant phenotype. We show that SAP is an F-box protein that forms part of a SKP1/Cullin/F-box E3 ubiquitin ligase complex and controls organ size by promoting the proliferation of meristemoid cells. Genetic analyses suggest that SAP may act in the same pathway with PEAPOD1 and PEAPOD2, which are negative regulators of meristemoid proliferation, to control organ size, but does so independently of DA1. Further results reveal that SAP physically associates with PEAPOD1 and PEAPOD2, and targets them for degradation. These findings define a molecular mechanism by which SAP and PEAPOD control organ size.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/genética
Proteínas de Ligação a DNA/genética
Regulação da Expressão Gênica no Desenvolvimento
Regulação da Expressão Gênica de Plantas
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Arabidopsis/citologia
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/metabolismo
Proteínas de Arabidopsis/metabolismo
Proliferação Celular
Proteínas Culina/genética
Proteínas Culina/metabolismo
Proteínas de Ligação a DNA/metabolismo
Proteínas F-Box/genética
Proteínas F-Box/metabolismo
Proteínas com Domínio LIM/genética
Proteínas com Domínio LIM/metabolismo
Meristema/citologia
Meristema/genética
Meristema/crescimento & desenvolvimento
Meristema/metabolismo
Tamanho do Órgão
Organogênese Vegetal/genética
Fenótipo
Células Vegetais/metabolismo
Complexo de Endopeptidases do Proteassoma/metabolismo
Proteólise
Proteínas Ligases SKP Culina F-Box/genética
Proteínas Ligases SKP Culina F-Box/metabolismo
Proteínas de Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/metabolismo
Transdução de Sinais
Fatores de Transcrição/metabolismo
Ubiquitina-Proteína Ligases/genética
Ubiquitina-Proteína Ligases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Cullin Proteins); 0 (DA1 protein, Arabidopsis); 0 (DNA-Binding Proteins); 0 (F-Box Proteins); 0 (LIM Domain Proteins); 0 (PEAPOD protein, Arabidopsis); 0 (SAP protein, Arabidopsis); 0 (Saccharomyces cerevisiae Proteins); 0 (TIFY4B protein, Arabidopsis); 0 (Transcription Factors); EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases); EC 2.3.2.27 (SKP1 protein, S cerevisiae); EC 2.3.2.27 (Ubiquitin-Protein Ligases); EC 3.4.25.1 (Proteasome Endopeptidase Complex)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160407
[St] Status:MEDLINE
[do] DOI:10.1038/ncomms11192


  8 / 63 MEDLINE  
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[PMID]:26906484
[Au] Autor:de Zeeuw T; Weijers D
[Ad] Endereço:Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, The Netherlands.
[Ti] Título:Plant Organogenesis: Rules of Order.
[So] Source:Curr Biol;26(4):R157-9, 2016 Feb 22.
[Is] ISSN:1879-0445
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plant lateral roots have a defined developmental pattern and shape, but a key question is whether strict regulation underlies observed regularity. A new study uses long-term in toto live imaging and simulations to show that organogenesis rather follows self-organizing principles.
[Mh] Termos MeSH primário: Organogênese Vegetal
Raízes de Plantas
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas
Organogênese
[Pt] Tipo de publicação:COMMENT; JOURNAL ARTICLE
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160224
[Lr] Data última revisão:
160224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE


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[PMID]:26872130
[Au] Autor:Adibi M; Yoshida S; Weijers D; Fleck C
[Ad] Endereço:Laboratory of Systems and Synthetic Biology, Wageningen University, Wageningen, the Netherlands.
[Ti] Título:Centering the Organizing Center in the Arabidopsis thaliana Shoot Apical Meristem by a Combination of Cytokinin Signaling and Self-Organization.
[So] Source:PLoS One;11(2):e0147830, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plants have the ability to continously generate new organs by maintaining populations of stem cells throught their lives. The shoot apical meristem (SAM) provides a stable environment for the maintenance of stem cells. All cells inside the SAM divide, yet boundaries and patterns are maintained. Experimental evidence indicates that patterning is independent of cell lineage, thus a dynamic self-regulatory mechanism is required. A pivotal role in the organization of the SAM is played by the WUSCHEL gene (WUS). An important question in this regard is that how WUS expression is positioned in the SAM via a cell-lineage independent signaling mechanism. In this study we demonstrate via mathematical modeling that a combination of an inhibitor of the Cytokinin (CK) receptor, Arabidopsis histidine kinase 4 (AHK4) and two morphogens originating from the top cell layer, can plausibly account for the cell lineage-independent centering of WUS expression within SAM. Furthermore, our laser ablation and microsurgical experiments support the hypothesis that patterning in SAM occurs at the level of CK reception and signaling. The model suggests that the interplay between CK signaling, WUS/CLV feedback loop and boundary signals can account for positioning of the WUS expression, and provides directions for further experimental investigation.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/genética
Citocininas/metabolismo
Regulação da Expressão Gênica de Plantas
Proteínas de Homeodomínio/genética
Meristema/genética
Proteínas Quinases/genética
Receptores de Superfície Celular/genética
[Mh] Termos MeSH secundário: Arabidopsis/citologia
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/metabolismo
Proteínas de Arabidopsis/metabolismo
Retroalimentação Fisiológica
Proteínas de Homeodomínio/metabolismo
Meristema/citologia
Meristema/crescimento & desenvolvimento
Meristema/metabolismo
Modelos Estatísticos
Organogênese Vegetal/genética
Células Vegetais/metabolismo
Proteínas Quinases/metabolismo
Proteínas Serina-Treonina Quinases
Receptores Proteína Tirosina Quinases/genética
Receptores Proteína Tirosina Quinases/metabolismo
Receptores de Superfície Celular/metabolismo
Transdução de Sinais
Células-Tronco/citologia
Células-Tronco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Cytokinins); 0 (Homeodomain Proteins); 0 (Receptors, Cell Surface); 0 (WUSCHEL protein, Arabidopsis); EC 2.7.- (Protein Kinases); EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases); EC 2.7.11.1 (CLV1 protein, Arabidopsis); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.3.- (WOL protein, Arabidopsis)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170323
[Lr] Data última revisão:
170323
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160213
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0147830


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[PMID]:26848843
[Au] Autor:Xu G; Huang J; Yang Y; Yao YA
[Ad] Endereço:Research Institute of Forest Resources and Environment, Guizhou University, Guiyang, Guizhou, P. R. China.
[Ti] Título:Transcriptome Analysis of Flower Sex Differentiation in Jatropha curcas L. Using RNA Sequencing.
[So] Source:PLoS One;11(2):e0145613, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Jatropha curcas is thought to be a promising biofuel material, but its yield is restricted by a low ratio of instaminate/staminate flowers (1/10-1/30). Furthermore, valuable information about flower sex differentiation in this plant is scarce. To explore the mechanism of this process in J. curcas, transcriptome profiling of flower development was carried out, and certain genes related with sex differentiation were obtained through digital gene expression analysis of flower buds from different phases of floral development. RESULTS: After Illumina sequencing and clustering, 57,962 unigenes were identified. A total of 47,423 unigenes were annotated, with 85 being related to carpel and stamen differentiation, 126 involved in carpel and stamen development, and 592 functioning in the later development stage for the maturation of staminate or instaminate flowers. Annotation of these genes provided comprehensive information regarding the sex differentiation of flowers, including the signaling system, hormone biosynthesis and regulation, transcription regulation and ubiquitin-mediated proteolysis. A further expression pattern analysis of 15 sex-related genes using quantitative real-time PCR revealed that gibberellin-regulated protein 4-like protein and AMP-activated protein kinase are associated with stamen differentiation, whereas auxin response factor 6-like protein, AGAMOUS-like 20 protein, CLAVATA1, RING-H2 finger protein ATL3J, auxin-induced protein 22D, and r2r3-myb transcription factor contribute to embryo sac development in the instaminate flower. Cytokinin oxidase, Unigene28, auxin repressed-like protein ARP1, gibberellin receptor protein GID1 and auxin-induced protein X10A are involved in both stages mentioned above. In addition to its function in the differentiation and development of the stamens, the gibberellin signaling pathway also functions in embryo sac development for the instaminate flower. The auxin signaling pathway also participates in both stamen development and embryo sac development. CONCLUSIONS: Our transcriptome data provide a comprehensive gene expression profile for flower sex differentiation in Jatropha curcas, as well as new clues and information for further study in this field.
[Mh] Termos MeSH primário: Flores/genética
Jatropha/genética
Transcriptoma
[Mh] Termos MeSH secundário: Biologia Computacional/métodos
Bases de Dados Genéticas
Flores/ultraestrutura
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Sequenciamento de Nucleotídeos em Larga Escala
Anotação de Sequência Molecular
Organogênese Vegetal/genética
Fenótipo
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160206
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0145613



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