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[PMID]: | 29174386 |
[Au] Autor: | Li T; Lu Z; Luo R; Gao J; Zhao X; Ma Y |
[Ad] Endereço: | College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; Sheep Breeding Biotechnology Engineering Laboratory of Gansu Province, Minqin 733300, China. |
[Ti] Título: | Expression and cellular localization of double sex and mab-3 related transcription factor 1 in testes of postnatal Small-Tail Han sheep at different developmental stages. |
[So] Source: | Gene;642:467-473, 2018 Feb 05. | [Is] ISSN: | 1879-0038 |
[Cp] País de publicação: | Netherlands |
[La] Idioma: | eng |
[Ab] Resumo: | Double sex and mab-3 related transcription factor 1 (Dmrt1), an evolutionarily conserved gene, is a sex-related gene expressed in male gonads, that is involved in the regulation of sex differentiation, testicular development and reproductive function maintenance. Until now, functional studies on the Dmrt1 gene in sheep (Ovis aries) have been lacking. In this study, testis, heart, liver, spleen, lung, kidney and longissimus dorsi muscle tissues were collected from Small-Tail Han sheep at 0, 2, 5, 12 and 24months after birth (mab). Dmrt1 expression and cellular localization were detected in various testicular tissues by quantitative real time PCR (qRT-PCR), western blot and immunohistochemistry methods. The morphological structures of testicular tissues at different developmental stages were observed by hematoxylin & eosin (HE) staining. The Dmrt1 mRNA expression levels in 12 and 24 mab sheep were significantly higher than those in 0 and 2 mab sheep (P<0.05), and Dmrt1 protein expression showed a similar trend. The qRT-PCR results in various tissues at 12 mab showed that Dmrt1 mRNA was predominantly expressed in testes. Immunohistochemical staining in testes at different developmental stages showed that Dmrt1 protein immunoreactive responses were mainly localized in Sertoli cells and gonocytes at 0, 2 and 5 mab, while they were localized in spermatocytes, sperm cells and some spermatogonia and Sertoli cells at 12 and 24 mab. We speculate that the Dmrt1 gene plays a vital role in postnatal sheep spermatogenesis, perhaps by regulating the maturation and functional maintenance of Sertoli cells, the proliferation and differentiation of gonocytes in prepubertal sheep testes, and the mitosis and meiosis of germ cells in adult sheep, but the specific mechanisms underlying these phenomena must be further studied and verified. |
[Mh] Termos MeSH primário: |
Ovinos/crescimento & desenvolvimento Testículo/crescimento & desenvolvimento Fatores de Transcrição/genética Fatores de Transcrição/metabolismo
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[Mh] Termos MeSH secundário: |
Animais Animais Recém-Nascidos Proliferação Celular Regulação da Expressão Gênica no Desenvolvimento Masculino Células de Sertoli/metabolismo Diferenciação Sexual Ovinos/genética Ovinos/metabolismo Espermatócitos/citologia Espermatócitos/metabolismo Espermatogênese Testículo/metabolismo
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Transcription Factors) |
[Em] Mês de entrada: | 1712 |
[Cu] Atualização por classe: | 171226 |
[Lr] Data última revisão:
| 171226 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 171128 |
[St] Status: | MEDLINE |
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