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Pesquisa : G07.345.625.124 [Categoria DeCS]
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[PMID]:28617857
[Au] Autor:Xiong B; Gu X; Qiu X; Dong Z; Ye S; Sun G; Huang S; Liu X; Xi L; Wang Z
[Ad] Endereço:College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
[Ti] Título:Variability in CitXET expression and XET activity in Citrus cultivar Huangguogan seedlings with differed degrees of etiolation.
[So] Source:PLoS One;12(6):e0178973, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Considering the known effects of xyloglucan endotransglycosylase (XET) on plant growth and development, we aimed to determine whether XETs help to regulate the growth and elongation of Huangguogan shoots and roots. We confirmed a possible role for XET during seedling etiolation. Our results revealed that the roots of etiolated seedlings (H-E) were longer than those of green seedlings (H-G). However, shoot length exhibited the opposite pattern. We also observed positive and negative effects on the xyloglucan-degrading activity of XET in the root sub-apical region and shoots of etiolated Huangguogan seedling, respectively. There was a significant down-regulation in CitXET expression in the etiolated shoots at 15 days after seed germination. On the contrary, it was significantly increased in the root sub-apical region of etiolated and multicolored seedlings at 15 days after seed germination. The XET coding sequence (i.e., CitXET) was cloned from Huangguogan seedlings using gene-specific primers. The encoded amino acid sequence was predicted by using bioinformatics-based methods. The 990-bp CitXET gene was highly homologous to other XET genes. The CitXET protein was predicted to contain 319 amino acids, with a molecular mass of 37.45 kDa and an isoelectric point of 9.05. The predicted molecular formula was C1724H2548N448O466S14, and the resulting protein included only one transmembrane structure. The CitXET secondary structure consisted of four main structures (i.e., 21% α-helix, 30.72% extended strand, 9.09% ß-turn, and 39.18% random coil). Analyses involving the NCBI Conserved Domains Database (NCBI-CDD), InterPro, and ScanProsite revealed that CitXET was a member of the glycosyl hydrolase family 16 (GH16), and included the DEIDFEFLG motif. Our results indicate that the differed degrees of etiolation influenced the CitXET expression pattern and XET activity in Huangguogan seedlings. The differential changes in XET activity and CitXET expression levels in Huangguogan seedlings may influence the regulation of root and shoot development, and may be important for seedling etiolation.
[Mh] Termos MeSH primário: Citrus/crescimento & desenvolvimento
Glicosiltransferases/genética
Glicosiltransferases/metabolismo
Plântulas/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Citrus/genética
Citrus/metabolismo
Clonagem Molecular
Estiolamento
Regulação da Expressão Gênica de Plantas
Germinação
Glicosiltransferases/química
Filogenia
Proteínas de Plantas/química
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Raízes de Plantas/genética
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Brotos de Planta/genética
Brotos de Planta/crescimento & desenvolvimento
Brotos de Planta/metabolismo
Estrutura Terciária de Proteína
Plântulas/genética
Plântulas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); EC 2.4.- (Glycosyltransferases); EC 2.4.1.- (xyloglucan endotransglycosylase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178973


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[PMID]:28552939
[Au] Autor:Talar U; Kielbowicz-Matuk A; Czarnecka J; Rorat T
[Ad] Endereço:Department of Environmental Stress Biology, Institute of Plant Genetics, Polish Academy of Sciences, Poznan, Poland.
[Ti] Título:Genome-wide survey of B-box proteins in potato (Solanum tuberosum)-Identification, characterization and expression patterns during diurnal cycle, etiolation and de-etiolation.
[So] Source:PLoS One;12(5):e0177471, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant B-box domain proteins (BBX) mediate many light-influenced developmental processes including seedling photomorphogenesis, seed germination, shade avoidance and photoperiodic regulation of flowering. Despite the wide range of potential functions, the current knowledge regarding BBX proteins in major crop plants is scarce. In this study, we identify and characterize the StBBX gene family in potato, which is composed of 30 members, with regard to structural properties and expression profiles under diurnal cycle, etiolation and de-etiolations. Based on domain organization and phylogenetic relationships, StBBX genes have been classified into five groups. Using real-time quantitative PCR, we found that expression of most of them oscillates following a 24-h rhythm; however, large differences in expression profiles were observed between the genes regarding amplitude and position of the maximal and minimal expression levels in the day/night cycle. On the basis of the time-of-day/time-of-night, we distinguished three expression groups specifically expressed during the light and two during the dark phase. In addition, we showed that the expression of several StBBX genes is under the control of the circadian clock and that some others are specifically associated with the etiolation and de-etiolation conditions. Thus, we concluded that StBBX proteins are likely key players involved in the complex diurnal and circadian networks regulating plant development as a function of light conditions and day duration.
[Mh] Termos MeSH primário: Ritmo Circadiano
Estiolamento
Genoma de Planta
Proteínas de Plantas/genética
Solanum tuberosum/metabolismo
[Mh] Termos MeSH secundário: Filogenia
Proteínas de Plantas/classificação
Solanum tuberosum/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170530
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177471


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[PMID]:28235420
[Au] Autor:Xu J; Li Y; Wang Y; Liu X; Zhu XG
[Ad] Endereço:Key Laboratory of Computational Biology and Partner Institute for Computational Biology, Chinese Academy of Sciences, Shanghai, China.
[Ti] Título:Altered expression profiles of microRNA families during de-etiolation of maize and rice leaves.
[So] Source:BMC Res Notes;10(1):108, 2017 Feb 24.
[Is] ISSN:1756-0500
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: MicroRNAs (miRNAs) are highly conserved small non-coding RNAs that play important regulatory roles in plants. Although many miRNA families are sequentially and functionally conserved across plant kingdoms (Dezulian et al. in Genome Biol 13, 2005), they still differ in many aspects such as family size, average length, genomic loci etc. (Unver et al. in Int J Plant Genomics, 2009). RESULTS: In this study, we investigated changes of miRNA expression profiles during greening process of etiolated seedlings of Oryza sativa (C ) and Zea mays (C ) to explore conserved and species-specific characteristics of miRNAs between these two species. Futhermore, we predicted 47 and 42 candidate novel miRNAs using parameterized monocot specific miRDeep2 pipeline in maize and rice respectively. Potential targets of miRNAs comprising both mRNA and long non-coding RNA (lncRNA) were examined to clarify potential regulation of photosynthesis. Based on our result, two putative positive Kranz regulators reported by Wang et al. (2010) were predicted as potential targets of miR156. A few photosynthesis related genes such as sulfate adenylytransferase (APS3), chlorophyll a/b binding family protein etc. were suggested to be regulated by miRNAs. However, no C shuttle genes were predicted to be direct targets of either known or candidate novel miRNAs. CONCLUSIONS: This study provided the comprehensive list of miRNA that showed altered expression during the de-etiolation process and a number of candidate miRNAs that might play regulatory roles in C and C photosynthesis.
[Mh] Termos MeSH primário: Estiolamento/genética
Regulação da Expressão Gênica de Plantas/fisiologia
MicroRNAs/genética
Oryza/genética
Transcriptoma/genética
Zea mays/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170310
[Lr] Data última revisão:
170310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE
[do] DOI:10.1186/s13104-016-2367-x


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[PMID]:27916965
[Au] Autor:Li YX; Pan YG; He FP; Yuan MQ; Li SB
[Ad] Endereço:College of Food, Hainan University, Haikou 570228, China. lisa19910@163.com.
[Ti] Título:Pathway Analysis and Metabolites Identification by Metabolomics of Etiolation Substrate from Fresh-Cut Chinese Water Chestnut (Eleocharis tuberosa).
[So] Source:Molecules;21(12), 2016 Dec 01.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Fresh-cut Chinese water chestnuts (CWC) turn yellow after being peeled, reducing their shelf life and commercial value. Metabolomics, the systematic study of the full complement of small molecular metabolites, was useful for clarifying the mechanism of fresh-cut CWC etiolation and developing methods to inhibit yellowing. In this study, metabolic alterations associated with etiolation at different growth stages (0 day, 2 days, 3 days, 4 days, 5 days) from fresh-cut CWC were investigated using LC-MS and analyzed by pattern recognition methods (principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and orthogonal projection to latent structures-discriminant analysis (OPLS-DA)). The metabolic pathways of the etiolation molecules were elucidated. The main metabolic pathway appears to be the conversion of phenylalanine to -coumaroyl-CoA, followed by conversion to naringenin chalcone, to naringenin, and naringenin then following different pathways. Firstly, it can transform into apigenin and its derivatives; secondly, it can produce eriodictyol and its derivatives; and thirdly it can produce dihydrokaempferol, quercetin, and myricetin. The eriodictyol can be further transformed to luteolin, cyanidin, dihydroquercetin, dihydrotricetin, and others. This is the first reported use of metabolomics to study the metabolic pathways of the etiolation of fresh-cut CWC.
[Mh] Termos MeSH primário: Eleocharis/metabolismo
Estiolamento/fisiologia
Metaboloma/fisiologia
Metabolômica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161206
[St] Status:MEDLINE


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[PMID]:27728837
[Au] Autor:Dümmer M; Michalski C; Essen LO; Rath M; Galland P; Forreiter C
[Ad] Endereço:Fachbereich Biologie, Philipps-Universität Marburg, Karl-von-Frisch Str. 8, D-35032 Marburg, Germany. Electronic address: duemmer@Staff.Uni-Marburg.DE.
[Ti] Título:EHB1 and AGD12, two calcium-dependent proteins affect gravitropism antagonistically in Arabidopsis thaliana.
[So] Source:J Plant Physiol;206:114-124, 2016 Nov 01.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The ADP-RIBOSYLATION FACTOR GTPase-ACTIVATING PROTEIN (AGD) 12, a member of the ARF-GAP protein family, affects gravitropism in Arabidopsis thaliana. A loss-of-function mutant lacking AGD12 displayed diminished gravitropism in roots and hypocotyls indicating that both organs are affected by this regulator. AGD12 is structurally related to ENHANCED BENDING (EHB) 1, previously described as a negative effector of gravitropism. In contrast to agd12 mutants, ehb1 loss-of function seedlings displayed enhanced gravitropic bending. While EHB1 and AGD12 both possess a C-terminal C2/CaLB-domain, EHB1 lacks the N-terminal ARF-GAP domain present in AGD12. Subcellular localization analysis using Brefeldin A indicated that both proteins are elements of the trans Golgi network. Physiological analyses provided evidence that gravitropic signaling might operate via an antagonistic interaction of ARF-GAP (AGD12) and EHB1 in their Ca -activated states.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/fisiologia
Cálcio/farmacologia
Proteínas Ativadoras de GTPase/metabolismo
Gravitropismo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/efeitos dos fármacos
Proteínas de Arabidopsis/química
Estiolamento/efeitos dos fármacos
Proteínas Ativadoras de GTPase/química
Meristema/efeitos dos fármacos
Meristema/fisiologia
Mutação/genética
Domínios Proteicos
Plântulas/efeitos dos fármacos
Plântulas/fisiologia
Frações Subcelulares/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AGD12 protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (EHB1 protein, Arabidopsis); 0 (GTPase-Activating Proteins); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170827
[Lr] Data última revisão:
170827
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161012
[St] Status:MEDLINE


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[PMID]:27485473
[Au] Autor:Boex-Fontvieille E; Rustgi S; Von Wettstein D; Pollmann S; Reinbothe S; Reinbothe C
[Ad] Endereço:a Laboratoire de Génétique Moléculaire des Plantes and Biologie Environnementale et Systémique (BEeSy), Université Grenoble-Alpes , Grenoble cedex , France.
[Ti] Título:Jasmonic acid protects etiolated seedlings of Arabidopsis thaliana against herbivorous arthropods.
[So] Source:Plant Signal Behav;11(8):e1214349, 2016 Aug 02.
[Is] ISSN:1559-2324
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Seed predators can cause mass ingestion of larger seed populations. As well, herbivorous arthropods attempt to attack etiolated seedlings and chose the apical hook for ingestion, aimed at dropping the cotyledons for later consumption. Etiolated seedlings, as we show here, have established an efficient mechanism of protecting their Achilles' heel against these predators, however. Evidence is provided for a role of jasmonic acid (JA) in this largely uncharacterized plant-herbivore interaction during skotomorphogenesis and that this comprises the temporally and spatially tightly controlled synthesis of a cysteine protease inhibitors of the Kunitz family. Interestingly, the same Kunitz protease inhibitor was found to be expressed in flowers of Arabidopsis where endogenous JA levels are high for fertility. Because both the apical hook and inflorescences were preferred isopod targets in JA-deficient plants that could be rescued by exogenously administered JA, our data identify a JA-dependent mechanism of plant arthropod deterrence that is recalled in different organs and at quite different times of plant development.
[Mh] Termos MeSH primário: Arabidopsis/metabolismo
Arabidopsis/parasitologia
Artrópodes/patogenicidade
Ciclopentanos/farmacologia
Herbivoria/fisiologia
Oxilipinas/farmacologia
Plântulas/metabolismo
[Mh] Termos MeSH secundário: Acetatos/farmacologia
Animais
Arabidopsis/efeitos dos fármacos
Estiolamento/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Plântulas/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Cyclopentanes); 0 (Oxylipins); 6RI5N05OWW (jasmonic acid); 900N171A0F (methyl jasmonate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160804
[St] Status:MEDLINE
[do] DOI:10.1080/15592324.2016.1214349


  7 / 53 MEDLINE  
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[PMID]:27436282
[Au] Autor:Xu J; Bräutigam A; Weber AP; Zhu XG
[Ad] Endereço:CAS Key Laboratory of Computational Biology and State Key Laboratory for Hybrid Rice, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
[Ti] Título:Systems analysis of cis-regulatory motifs in C4 photosynthesis genes using maize and rice leaf transcriptomic data during a process of de-etiolation.
[So] Source:J Exp Bot;67(17):5105-17, 2016 Sep.
[Is] ISSN:1460-2431
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Identification of potential cis-regulatory motifs controlling the development of C4 photosynthesis is a major focus of current research. In this study, we used time-series RNA-seq data collected from etiolated maize and rice leaf tissues sampled during a de-etiolation process to systematically characterize the expression patterns of C4-related genes and to further identify potential cis elements in five different genomic regions (i.e. promoter, 5'UTR, 3'UTR, intron, and coding sequence) of C4 orthologous genes. The results demonstrate that although most of the C4 genes show similar expression patterns, a number of them, including chloroplast dicarboxylate transporter 1, aspartate aminotransferase, and triose phosphate transporter, show shifted expression patterns compared with their C3 counterparts. A number of conserved short DNA motifs between maize C4 genes and their rice orthologous genes were identified not only in the promoter, 5'UTR, 3'UTR, and coding sequences, but also in the introns of core C4 genes. We also identified cis-regulatory motifs that exist in maize C4 genes and also in genes showing similar expression patterns as maize C4 genes but that do not exist in rice C3 orthologs, suggesting a possible recruitment of pre-existing cis-elements from genes unrelated to C4 photosynthesis into C4 photosynthesis genes during C4 evolution.
[Mh] Termos MeSH primário: Estiolamento/genética
Genes de Plantas/genética
Oryza/genética
Fotossíntese/genética
Folhas de Planta/fisiologia
Zea mays/genética
[Mh] Termos MeSH secundário: Estiolamento/fisiologia
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas/genética
Regulação da Expressão Gênica de Plantas/fisiologia
Genes de Plantas/fisiologia
Oryza/fisiologia
Fotossíntese/fisiologia
Zea mays/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE
[do] DOI:10.1093/jxb/erw275


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[PMID]:27208292
[Au] Autor:Zang G; Zou H; Zhang Y; Xiang Z; Huang J; Luo L; Wang C; Lei K; Li X; Song D; Din AU; Wang G
[Ad] Endereço:Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China (G.Z., H.Z., Z.X., J.H., L.L., A.U.D., G.W.);Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
[Ti] Título:The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice.
[So] Source:Plant Physiol;171(2):1259-76, 2016 Jun.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DEETIOLATED1 (DET1) plays a critical role in developmental and environmental responses in many plants. To date, the functions of OsDET1 in rice (Oryza sativa) have been largely unknown. OsDET1 is an ortholog of Arabidopsis (Arabidopsis thaliana) DET1 Here, we found that OsDET1 is essential for maintaining normal rice development. The repression of OsDET1 had detrimental effects on plant development, and leaded to contradictory phenotypes related to abscisic acid (ABA) in OsDET1 interference (RNAi) plants. We found that OsDET1 is involved in modulating ABA signaling in rice. OsDET1 RNAi plants exhibited an ABA hypersensitivity phenotype. Using yeast two-hybrid (Y2H) and bimolecular fluorescence complementation assays, we determined that OsDET1 interacts physically with DAMAGED-SPECIFIC DNA-BINDING PROTEIN1 (OsDDB1) and CONSTITUTIVE PHOTOMORPHOGENIC10 (COP10); DET1- and DDB1-ASSOCIATED1 binds to the ABA receptors OsPYL5 and OsDDB1. We found that the degradation of OsPYL5 was delayed in OsDET1 RNAi plants. These findings suggest that OsDET1 deficiency disturbs the COP10-DET1-DDB1 complex, which is responsible for ABA receptor (OsPYL) degradation, eventually leading to ABA sensitivity in rice. Additionally, OsDET1 also modulated ABA biosynthesis, as ABA biosynthesis was inhibited in OsDET1 RNAi plants and promoted in OsDET1-overexpressing transgenic plants. In conclusion, our data suggest that OsDET1 plays an important role in maintaining normal development in rice and mediates the cross talk between ABA biosynthesis and ABA signaling pathways in rice.
[Mh] Termos MeSH primário: Ácido Abscísico/biossíntese
Proteínas de Arabidopsis/química
Estiolamento
Proteínas Nucleares/química
Oryza/metabolismo
Proteínas de Plantas/metabolismo
Homologia de Sequência de Aminoácidos
Transdução de Sinais
[Mh] Termos MeSH secundário: Ácido Abscísico/farmacologia
Escuridão
Estiolamento/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Pleiotropia Genética/efeitos dos fármacos
Germinação/efeitos dos fármacos
Germinação/genética
Proteínas de Fluorescência Verde/metabolismo
Complexos Multiproteicos/metabolismo
Oryza/efeitos dos fármacos
Oryza/genética
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/genética
Proteínas de Plantas/genética
Plantas Geneticamente Modificadas
Ligação Proteica/efeitos dos fármacos
Proteólise/efeitos dos fármacos
Interferência de RNA/efeitos dos fármacos
Plântulas/efeitos dos fármacos
Plântulas/genética
Plântulas/crescimento & desenvolvimento
Transdução de Sinais/efeitos dos fármacos
Transdução de Sinais/genética
Estresse Fisiológico/efeitos dos fármacos
Estresse Fisiológico/genética
Transcriptoma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (DET1 protein, Arabidopsis); 0 (Multiprotein Complexes); 0 (Nuclear Proteins); 0 (Plant Proteins); 147336-22-9 (Green Fluorescent Proteins); 72S9A8J5GW (Abscisic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160522
[St] Status:MEDLINE
[do] DOI:10.1104/pp.16.00059


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[PMID]:27090636
[Au] Autor:Hlousková P; Bergougnoux V
[Ad] Endereço:Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research and Faculty of Science, Palacký University in Olomouc, Slechtitelu 11, CZ-783 71, Olomouc, Czech Republic.
[Ti] Título:A subtracted cDNA library identifies genes up-regulated during PHOT1-mediated early step of de-etiolation in tomato (Solanum lycopersicum L.).
[So] Source:BMC Genomics;17:291, 2016 Apr 18.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: De-etiolation is the switch from skoto- to photomorphogenesis, enabling the heterotrophic etiolated seedling to develop into an autotrophic plant. Upon exposure to blue light (BL), reduction of hypocotyl growth rate occurs in two phases: a rapid inhibition mediated by phototropin 1 (PHOT1) within the first 30-40 min of illumination, followed by the cryptochrome 1 (CRY1)-controlled establishment of the steady-state growth rate. Although some information is available for CRY1-mediated de-etiolation, less attention has been given to the PHOT1 phase of de-etiolation. RESULTS: We generated a subtracted cDNA library using the suppression subtractive hybridization method to investigate the molecular mechanisms of BL-induced de-etiolation in tomato (Solanum lycopersicum L.), an economically important crop. We focused our interest on the first 30 min following the exposure to BL when PHOT1 is required to induce the process. Our library generated 152 expressed sequence tags that were found to be rapidly accumulated upon exposure to BL and consequently potentially regulated by PHOT1. Annotation revealed that biological functions such as modification of chromatin structure, cell wall modification, and transcription/translation comprise an important part of events contributing to the establishment of photomorphogenesis in young tomato seedlings. Our conclusions based on bioinformatics data were supported by qRT-PCR analyses the specific investigation of V-H(+)-ATPase during de-etiolation in tomato. CONCLUSIONS: Our study provides the first report dealing with understanding the PHOT1-mediated phase of de-etiolation. Using subtractive cDNA library, we were able to identify important regulatory mechanisms. The profound induction of transcription/translation, as well as modification of chromatin structure, is relevant in regard to the fact that the entry into photomorphogenesis is based on a deep reprograming of the cell. Also, we postulated that BL restrains the cell expansion by the rapid modification of the cell wall.
[Mh] Termos MeSH primário: Estiolamento/genética
Luz
Lycopersicon esculentum/genética
Fototropinas/fisiologia
[Mh] Termos MeSH secundário: Cromatina/ultraestrutura
Regulação da Expressão Gênica de Plantas
Biblioteca Gênica
Redes Reguladoras de Genes
Hipocótilo/crescimento & desenvolvimento
Lycopersicon esculentum/crescimento & desenvolvimento
Plântulas/genética
Plântulas/crescimento & desenvolvimento
Regulação para Cima
ATPases Vacuolares Próton-Translocadoras/genética
ATPases Vacuolares Próton-Translocadoras/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chromatin); 0 (Phototropins); EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160420
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-016-2613-6


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[PMID]:26969722
[Au] Autor:Chen S; Jia H; Zhao H; Liu D; Liu Y; Liu B; Bauer S; Somerville CR
[Ad] Endereço:Biomass Energy Center for Arid and Semi-arid Lands, Northwest A&F University, Shaanxi, China (S.C., H.J., H.Z., D.L., Y.L., B.L.);College of Life Sciences, Northwest A&F University, Shaanxi, China (S.C., H.J.);Energy Biosciences Institute (S.B., C.R.S.), andDepartment of Plant and Microbial
[Ti] Título:Anisotropic Cell Expansion Is Affected through the Bidirectional Mobility of Cellulose Synthase Complexes and Phosphorylation at Two Critical Residues on CESA3.
[So] Source:Plant Physiol;171(1):242-50, 2016 May.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Here we report that phosphorylation status of S211 and T212 of the CESA3 component of Arabidopsis (Arabidopsis thaliana) cellulose synthase impacts the regulation of anisotropic cell expansion as well as cellulose synthesis and deposition and microtubule-dependent bidirectional mobility of CESA complexes. Mutation of S211 to Ala caused a significant decrease in the length of etiolated hypocotyls and primary roots, while root hairs were not significantly affected. By contrast, the S211E mutation stunted the growth of root hairs, but primary roots were not significantly affected. Similarly, T212E caused a decrease in the length of root hairs but not root length. However, T212E stunted the growth of etiolated hypocotyls. Live-cell imaging of fluorescently labeled CESA showed that the rate of movement of CESA particles was directionally asymmetric in etiolated hypocotyls of S211A and T212E mutants, while similar bidirectional velocities were observed with the wild-type control and S211E and T212A mutant lines. Analysis of cell wall composition and the innermost layer of cell wall suggests a role for phosphorylation of CESA3 S211 and T212 in cellulose aggregation into fibrillar bundles. These results suggest that microtubule-guided bidirectional mobility of CESA complexes is fine-tuned by phosphorylation of CESA3 S211 and T212, which may, in turn, modulate cellulose synthesis and organization, resulting in or contributing to the observed defects of anisotropic cell expansion.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/enzimologia
Arabidopsis/genética
Glucosiltransferases/metabolismo
Fosforilação
[Mh] Termos MeSH secundário: Anisotropia
Arabidopsis/citologia
Proteínas de Arabidopsis/genética
Parede Celular/metabolismo
Celulose/metabolismo
DNA Complementar
Dinitrobenzenos
Estiolamento
Glucosiltransferases/genética
Hipocótilo/metabolismo
Microscopia de Força Atômica
Microscopia Eletrônica de Varredura
Microtúbulos/metabolismo
Monossacarídeos/análise
Mutagênese Sítio-Dirigida
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Plantas Geneticamente Modificadas
Plântulas/crescimento & desenvolvimento
Sulfanilamidas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (DNA, Complementary); 0 (Dinitrobenzenes); 0 (Monosaccharides); 0 (Sulfanilamides); 662E385DWH (oryzalin); 9004-34-6 (Cellulose); EC 2.4.1.- (CesA3 protein, Arabidopsis); EC 2.4.1.- (Glucosyltransferases); EC 2.4.1.- (cellulose synthase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160313
[St] Status:MEDLINE
[do] DOI:10.1104/pp.15.01874



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