Base de dados : MEDLINE
Pesquisa : G07.345.625.875 [Categoria DeCS]
Referências encontradas : 421 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 43 ir para página                         

  1 / 421 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28981580
[Au] Autor:Velappan Y; Signorelli S; Considine MJ
[Ad] Endereço:The UWA Institute of Agriculture, The University of Western Australia, Perth, WA 6009, Australia.
[Ti] Título:Cell cycle arrest in plants: what distinguishes quiescence, dormancy and differentiated G1?
[So] Source:Ann Bot;120(4):495-509, 2017 Oct 17.
[Is] ISSN:1095-8290
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Background: Quiescence is a fundamental feature of plant life, which enables plasticity, renewal and fidelity of the somatic cell line. Cellular quiescence is defined by arrest in a particular phase of the cell cycle, typically G1 or G2; however, the regulation of quiescence and proliferation can also be considered across wider scales in space and time. As such, quiescence is a defining feature of plant development and phenology, from meristematic stem cell progenitors to terminally differentiated cells, as well as dormant or suppressed seeds and buds. While the physiology of each of these states differs considerably, each is referred to as 'cell cycle arrest' or 'G1 arrest'. Scope: Here the physiology and molecular regulation of (1) meristematic quiescence, (2) dormancy and (3) terminal differentiation (cell cycle exit) are considered in order to determine whether and how the molecular decisions guiding these nuclear states are distinct. A brief overview of the canonical cell cycle regulators is provided, and the genetic and genomic, as well as physiological, evidence is considered regarding two primary questions: (1) Are the canonical cell cycle regulators superior or subordinate in the regulation of quiescence? (2) Are these three modes of quiescence governed by distinct molecular controls? Conclusion: Meristematic quiescence, dormancy and terminal differentiation are each predominantly characterized by G1 arrest but regulated distinctly, at a level largely superior to the canonical cell cycle. Meristematic quiescence is intrinsically linked to non-cell-autonomous regulation of meristem cell identity, and particularly through the influence of ubiquitin-dependent proteolysis, in partnership with reactive oxygen species, abscisic acid and auxin. The regulation of terminal differentiation shares analogous features with meristematic quiescence, albeit with specific activators and a greater role for cytokinin signalling. Dormancy meanwhile appears to be regulated at the level of chromatin accessibility, by Polycomb group-type histone modifications of particular dormancy genes.
[Mh] Termos MeSH primário: Pontos de Checagem do Ciclo Celular/fisiologia
Desenvolvimento Vegetal/fisiologia
Dormência de Plantas/fisiologia
[Mh] Termos MeSH secundário: Fase G1/fisiologia
Meristema/crescimento & desenvolvimento
Meristema/fisiologia
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/fisiologia
Brotos de Planta/crescimento & desenvolvimento
Brotos de Planta/fisiologia
Plantas
Fase de Repouso do Ciclo Celular/fisiologia
Fase S/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1093/aob/mcx082


  2 / 421 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28628628
[Au] Autor:Nelson SK; Steber CM
[Ad] Endereço:Molecular Plant Sciences Program, Washington State University, Pullman, Washington, United States of America.
[Ti] Título:Transcriptional mechanisms associated with seed dormancy and dormancy loss in the gibberellin-insensitive sly1-2 mutant of Arabidopsis thaliana.
[So] Source:PLoS One;12(6):e0179143, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:While widespread transcriptome changes were previously observed with seed dormancy loss, this study specifically characterized transcriptional changes associated with the increased seed dormancy and dormancy loss of the gibberellin (GA) hormone-insensitive sleepy1-2 (sly1-2) mutant. The SLY1 gene encodes the F-box subunit of an SCF E3 ubiquitin ligase needed for GA-triggered proteolysis of DELLA repressors of seed germination. DELLA overaccumulation in sly1-2 seeds leads to increased dormancy that can be rescued without DELLA protein destruction either by overexpression of the GA receptor, GA-INSENSITIVE DWARF1b (GID1b-OE) (74% germination) or by extended dry after-ripening (11 months, 51% germination). After-ripening of sly1 resulted in different transcriptional changes in early versus late Phase II of germination that were consistent with the processes known to occur. Approximately half of the transcriptome changes with after-ripening appear to depend on SLY1-triggered DELLA proteolysis. Given that many of these SLY1/GA-dependent changes are genes involved in protein translation, it appears that GA signaling increases germination capacity in part by activating translation. While sly1-2 after-ripening was associated with transcript-level changes in 4594 genes over two imbibition timepoints, rescue of sly1-2 germination by GID1b-OE was associated with changes in only 23 genes. Thus, a big change in sly1-2 germination phenotype can occur with relatively little change in the global pattern of gene expression during the process of germination. Most GID1b-OE-responsive transcripts showed similar changes with after-ripening in early Phase II of imbibition, but opposite changes with after-ripening by late Phase II. This suggests that GID1b-OE stimulates germination early in imbibition, but may later trigger negative feedback regulation.
[Mh] Termos MeSH primário: Alquil e Aril Transferases/metabolismo
Proteínas de Arabidopsis/metabolismo
Arabidopsis/efeitos dos fármacos
Giberelinas/farmacologia
Dormência de Plantas/efeitos dos fármacos
Transcriptoma/efeitos dos fármacos
[Mh] Termos MeSH secundário: Alquil e Aril Transferases/genética
Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/genética
Germinação/efeitos dos fármacos
Mutação
Fenótipo
Reguladores de Crescimento de Planta/farmacologia
Plantas Geneticamente Modificadas/metabolismo
Proteólise
RNA de Plantas/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Receptores de Superfície Celular/genética
Receptores de Superfície Celular/metabolismo
Sementes/efeitos dos fármacos
Sementes/genética
Sementes/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (GAI protein, Arabidopsis); 0 (GID1b protein, Arabidopsis); 0 (Gibberellins); 0 (Plant Growth Regulators); 0 (RNA, Plant); 0 (Receptors, Cell Surface); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.29 (SLY1 protein, Arabidopsis)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179143


  3 / 421 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28411210
[Au] Autor:Imaizumi T; Auge G; Donohue K
[Ad] Endereço:Department of Biology, Duke University, Box 90338, Durham, North Carolina 27708 USA toima@affrc.go.jp.
[Ti] Título:Photoperiod throughout the maternal life cycle, not photoperiod during seed imbibition, influences germination in .
[So] Source:Am J Bot;104(4):516-526, 2017 Apr.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Plants adjust their phenology in response to seasonal cues experienced both by their parents and by themselves, and coordinating responses to these cues is necessary for expressing adaptive phenology. We investigated how cues are integrated across time to influence an important progeny phenotype, i.e., seed germination. METHODS: We used to investigate how the photoperiod experienced by maternal parents and by progeny influences seed germination. We examined when maternal photoperiod effects on germination are imposed and how long they persist in progeny. KEY RESULTS: The photoperiod experienced by maternal plants more strongly influenced germination than the photoperiod experienced during seed imbibition. In addition, the photoperiod experienced at the prereproductive stage frequently influenced germination as strongly as that experienced during reproduction. In general, seeds from plants grown under short days had higher seed germination percentages than seeds from plants grown in longer days. These maternal effects diminished with after-ripening, but reappeared in seeds induced into secondary dormancy. CONCLUSIONS: We found no evidence that the effect of photoperiod systematically attenuates in proportion to the time that elapsed between the cue and the timing of seed germination. Moreover, more recently experienced cues did not override the effects of cues experienced previously. Instead, specific sequences of photoperiods experienced at the prereproductive and reproductive stages appear to influence germination behavior.
[Mh] Termos MeSH primário: Arabidopsis/fisiologia
Germinação/fisiologia
Fotoperíodo
Sementes/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Arabidopsis/efeitos da radiação
Germinação/efeitos da radiação
Dormência de Plantas/fisiologia
Dormência de Plantas/efeitos da radiação
Reprodução/fisiologia
Reprodução/efeitos da radiação
Sementes/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1600389


  4 / 421 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28391330
[Au] Autor:Finch-Savage WE; Footitt S
[Ad] Endereço:School of Life Sciences, Wellesbourne Campus, University of Warwick, Warwickshire CV35 9EF, UK.
[Ti] Título:Seed dormancy cycling and the regulation of dormancy mechanisms to time germination in variable field environments.
[So] Source:J Exp Bot;68(4):843-856, 2017 Feb 01.
[Is] ISSN:1460-2431
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Many molecular mechanisms that regulate dormancy have been identified individually in controlled laboratory studies. However, little is known about how the seed employs this complex suite of mechanisms during dormancy cycling in the variable environment of the soil seed bank. Nevertheless, this behaviour is essential to ensure germination takes place in a favourable habitat and climate space, and in the correct season for the resulting plant to complete its life cycle. During their time in the soil seed bank, seeds continually adjust their dormancy status by sensing a range of environmental signals. Those related to slow seasonal change (e.g. temperature) are used for temporal sensing to determine the time of year and depth of dormancy. This alters their sensitivity to signals related to their spatial environment (e.g. light, nitrate, and water potential) that indicate that conditions are suitable for germination, and so trigger the termination of dormancy. We review work on the physiological, molecular, and ecological aspects of seed dormancy in Arabidopsis and interpret it in the context of dormancy cycling in the soil seed bank. This approach has provided new insight into the co-ordination of mechanisms and signalling networks, and the multidimensional sensing that regulates dormancy cycling in a variable environment.
[Mh] Termos MeSH primário: Dormência de Plantas/fisiologia
[Mh] Termos MeSH secundário: Arabidopsis/crescimento & desenvolvimento
Arabidopsis/fisiologia
Meio Ambiente
Regulação da Expressão Gênica de Plantas/fisiologia
Luz
Fenômenos Fisiológicos Vegetais
Estações do Ano
Água/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170410
[St] Status:MEDLINE
[do] DOI:10.1093/jxb/erw477


  5 / 421 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28365837
[Au] Autor:Chao WS; Dogramaci M; Horvath DP; Anderson JV; Foley ME
[Ad] Endereço:Biosciences Research Lab, USDA-Agricultural Research Service, 1605 Albrecht Boulevard N., Fargo, ND, 58102-2765, USA. wun.chao@ars.usda.gov.
[Ti] Título:Comparison of phytohormone levels and transcript profiles during seasonal dormancy transitions in underground adventitious buds of leafy spurge.
[So] Source:Plant Mol Biol;94(3):281-302, 2017 Jun.
[Is] ISSN:1573-5028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Leafy spurge (Euphorbia esula L.) is an herbaceous perennial weed that maintains its perennial growth habit through generation of underground adventitious buds (UABs) on the crown and lateral roots. These UABs undergo seasonal phases of dormancy under natural conditions, namely para-, endo-, and ecodormancy in summer, fall, and winter, respectively. These dormancy phases can also be induced in growth chambers by manipulating photoperiod and temperature. In this study, UABs induced into the three phases of dormancy under controlled conditions were used to compare changes in phytohormone and transcriptome profiles. Results indicated that relatively high levels of ABA, the ABA metabolite PA, and IAA were found in paradormant buds. When UABs transitioned from para- to endodormancy, ABA and PA levels decreased, whereas IAA levels were maintained. Additionally, transcript profiles associated with regulation of soluble sugars and ethylene activities were also increased during para- to endodormancy transition, which may play some role in maintaining endodormancy status. When crown buds transitioned from endo- to ecodormancy, the ABA metabolites PA and DPA decreased significantly along with the down-regulation of ABA biosynthesis genes, ABA2 and NCED3. IAA levels were also significantly lower in ecodormant buds than that of endodormant buds. We hypothesize that extended cold treatment may trigger physiological stress in endodormant buds, and that these stress-associated signals induced the endo- to ecodormancy transition and growth competence. The up-regulation of NAD/NADH phosphorylation and dephosphorylation pathway, and MAF3-like and GRFs genes, may be considered as markers of growth competency.
[Mh] Termos MeSH primário: Euphorbia/fisiologia
Dormência de Plantas/fisiologia
Reguladores de Crescimento de Planta/metabolismo
Proteínas de Plantas/metabolismo
Estações do Ano
Transcriptoma
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas/fisiologia
Proteínas de Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Growth Regulators); 0 (Plant Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170403
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-017-0607-7


  6 / 421 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28283703
[Au] Autor:Zhou Y; Xie Y; Cai J; Liu C; Zhu H; Jiang R; Zhong Y; Zhang G; Tan B; Liu G; Fu X; Liu Z; Wang S; Zhang G; Zeng R
[Ad] Endereço:State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources and Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
[Ti] Título:Substitution mapping of QTLs controlling seed dormancy using single segment substitution lines derived from multiple cultivated rice donors in seven cropping seasons.
[So] Source:Theor Appl Genet;130(6):1191-1205, 2017 Jun.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: A permanent advanced population containing 388 SSSLs was used for genetic analysis of seed dormancy; 25 QTLs including eight stable, six major and five new were identified. Seed dormancy (SD) is not only a complex biological phenomenon, but also a key practical problem in agricultural production closely related with pre-harvest sprouting (PHS). However, the genetic mechanisms of SD remain elusive. Here, we report the genetic dissection of SD in rice using 388 single segment substitution lines (SSSLs) derived from 16 donor parents. Continuous variation and positive correlations in seed germination percentages were observed in seven seasons. Genetic analysis revealed the narrow sense heritability in different seasons varied from 31.4 to 82.2% with an average value of 56.8%. In addition, 49 SSSLs exhibited significant difference to recipient parent HJX74 on SD in at least two seasons, and 12 of them were stably identified with putative QTLs in all of their corresponding cropping seasons. Based on substitution mapping, a total of 25 dormancy QTLs were detected on 11 chromosomes except the chromosome 5 with an interval length of 1.1 to 31.3 cM. The additive effects of these QTLs changed from -0.31 to -0.13, and the additive effect contributions ranged from 16.7 to 41.4%. Six QTLs, qSD3-2, qSD4-1, qSD7-1, qSD7-2, qSD7-3 and qSD11-2, showed large additive effect contributions (≥30%). Five QTLs, qSD3-3, qSD7-1, qSD7-4, qSD9-1 and qSD10-1, may represent novel ones. Furthermore, linkage and recombinant analysis delimited qSD7-1 to a locus 1.5 cM away from marker Oi2 and a 355-kb fragment flanked by RM1134 and Ui159, respectively. Taken together, this work conducts a comprehensive genetic dissection of SD and will provide more selections for breeding elite PHS-resistant rice varieties.
[Mh] Termos MeSH primário: Oryza/genética
Dormência de Plantas/genética
Locos de Características Quantitativas
[Mh] Termos MeSH secundário: Mapeamento Cromossômico
Ligação Genética
Variação Genética
Oryza/fisiologia
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170312
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2881-9


  7 / 421 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:28229967
[Au] Autor:Arif MA; Nagel M; Lohwasser U; Borner A
[Ad] Endereço:Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
[Ti] Título:Genetic architecture of seed longevity in bread wheat (Triticum aestivum L.).
[So] Source:J Biosci;42(1):81-89, 2017 Mar.
[Is] ISSN:0973-7138
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:The deterioration in the quality of ex situ conserved seed over time reflects a combination of both physical and chemical changes. Intraspecific variation for longevity is, at least in part, under genetic control. Here, the grain of 183 bread wheat accessions maintained under low-temperature storage at the IPK-Gatersleben genebank over some decades have been tested for their viability, along with that of fresh grain subjected to two standard artificial ageing procedures. A phenotype-genotype association analysis, conducted to reveal the genetic basis of the observed variation between accessions, implicated many regions of the genome, underling the genetic complexity of the trait. Some, but not all, of these regions were associated with variation for both natural and experimental ageing, implying some non-congruency obtains between these two forms of testing for longevity. The genes underlying longevity appear to be independent of known genes determining dormancy and pre-harvest sprouting.
[Mh] Termos MeSH primário: Longevidade/genética
Locos de Características Quantitativas/genética
Sementes/genética
Triticum/genética
[Mh] Termos MeSH secundário: Envelhecimento/genética
Estudos de Associação Genética
Germinação
Fenótipo
Dormência de Plantas/genética
Proteínas de Plantas/genética
Sementes/crescimento & desenvolvimento
Triticum/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170224
[St] Status:MEDLINE


  8 / 421 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28126845
[Au] Autor:Basbouss-Serhal I; Pateyron S; Cochet F; Leymarie J; Bailly C
[Ad] Endereço:Sorbonne Universités, UPMC Université Paris 06, Centre National de la Recherche Scientifique, Institut de Biologie Paris-Seine, Unité Mixte de Recherche 7622, Biologie du Développement, F-75005 Paris, France (I.B.-S., F.C., J.L., C.B.).
[Ti] Título:5' to 3' mRNA Decay Contributes to the Regulation of Arabidopsis Seed Germination by Dormancy.
[So] Source:Plant Physiol;173(3):1709-1723, 2017 Mar.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The regulation of plant gene expression, necessary for development and adaptive responses, relies not only on RNA transcription but also on messenger RNA (mRNA) fate. To understand whether seed germination relies on the degradation of specific subsets of mRNA, we investigated whether the 5' to 3' RNA decay machinery participated in the regulation of this process. Arabidopsis ( ) seeds of ( ) and ( ) mutants displayed distinct dormancy phenotypes. Transcriptome analysis and mutant seeds allowed us to identify genes that are likely to play a role in the control of germination. Study of 5' untranslated region features of these transcripts revealed that specific motifs, secondary energy, and GC content could play a role in their degradation by XRN4 and VCS, and Gene Ontology clustering revealed novel actors of seed dormancy and germination. Several specific transcripts identified as being putative targets of XRN4 and VCS in seeds ( , , , and ) were further studied by reverse genetics, and their functional roles in the germination process were confirmed by mutant analysis. These findings suggest that completion of germination and its regulation by dormancy also depend on the degradation of specific subsets of mRNA.
[Mh] Termos MeSH primário: Arabidopsis/genética
Regulação da Expressão Gênica de Plantas
Germinação/genética
Dormência de Plantas/genética
RNA Mensageiro/genética
Sementes/genética
[Mh] Termos MeSH secundário: Regiões 5' não Traduzidas/genética
Ácido Abscísico/metabolismo
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Composição de Bases/genética
Sequência de Bases
Análise por Conglomerados
Exorribonucleases/genética
Exorribonucleases/metabolismo
Perfilação da Expressão Gênica/métodos
Ontologia Genética
Mutação
Motivos de Nucleotídeos/genética
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Estabilidade de RNA/genética
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Sementes/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (Arabidopsis Proteins); 0 (Plant Proteins); 0 (RNA, Messenger); 0 (VARICOSE protein, Arabidopsis); 0 (Xrn1 protein, plant); 72S9A8J5GW (Abscisic Acid); EC 3.1.- (Exoribonucleases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE
[do] DOI:10.1104/pp.16.01933


  9 / 421 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28103799
[Au] Autor:Khalil-Ur-Rehman M; Sun L; Li CX; Faheem M; Wang W; Tao JM
[Ad] Endereço:Laboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
[Ti] Título:Comparative RNA-seq based transcriptomic analysis of bud dormancy in grape.
[So] Source:BMC Plant Biol;17(1):18, 2017 Jan 19.
[Is] ISSN:1471-2229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bud dormancy is an important biological phenomenon of perennial plants that enables them to survive under harsh environmental circumstances. Grape (Vitis vinifera) is one of the most grown fruit crop worldwide; however, underlying mechanisms involved in grape bud dormancy are not yet clear. This work was aimed to explore the underlying molecular mechanism regulating bud dormancy in grape. RESULTS: We have performed transcriptome and differential transcript expression analyses of "Shine Muscat" grape buds using the Illumina RNA-seq system. Comparisons of transcript expression levels among three stages of dormancy, paradormancy (PD) vs endodormancy (ED), summer buds (SB) vs ED and SB vs PD, resulted in the detection of 8949, 9780 and 3938 differentially expressed transcripts, respectively. Out of approximately 78 million high-quality generated reads, 6096 transcripts were differentially expressed (log2 ratio ≥ 1, FDR ≤ 0.001). Grape reference genome was used for alignment of sequence reads and to measure the expression level of transcripts. Furthermore, findings obtained were then compared using two different databases; Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), to annotate the transcript descriptions and to assign a pathway to each transcript. KEGG analysis revealed that secondary metabolites biosynthesis and plant hormone signaling was found most enriched out of the 127 total pathways. In the comparisons of the PD vs ED and SB vs ED stages of grape buds, the gibberellin (GA) and abscisic acid (ABA) pathways were found to be the most enriched. The ABA and GA pathways were further analyzed to observe the expression pattern of differentially expressed transcripts. Transcripts related to the PP2C family (ABA pathway) were found to be up-regulated in the PD vs ED comparison and down-regulated in the SB vs ED and SB vs PD comparisons. GID1 family transcripts (GA pathway) were up-regulated while DELLA family transcripts were down-regulated during the three dormancy stages. Differentially expressed transcripts (DEGs) related to redox activity were abundant in the GO biological process category. RT-qPCR assay results for 12 selected transcripts validated the data obtained by RNA-seq. CONCLUSION: At this stage, taking into account the results obtained so far, it is possible to put forward a hypothesis for the molecular mechanism underlying grape bud dormancy, which may pave the way for ultimate improvements in the grape industry.
[Mh] Termos MeSH primário: Proteínas de Plantas/genética
RNA de Plantas/genética
Vitis/genética
[Mh] Termos MeSH secundário: Flores/genética
Flores/crescimento & desenvolvimento
Flores/metabolismo
Perfilação da Expressão Gênica
Regulação da Expressão Gênica no Desenvolvimento
Regulação da Expressão Gênica de Plantas
Dormência de Plantas
Proteínas de Plantas/metabolismo
RNA de Plantas/metabolismo
Vitis/crescimento & desenvolvimento
Vitis/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (RNA, Plant)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE
[do] DOI:10.1186/s12870-016-0960-8


  10 / 421 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28087660
[Au] Autor:Fogliani B; Gâteblé G; Villegente M; Fabre I; Klein N; Anger N; Baskin CC; Scutt CP
[Ad] Endereço:Institut Agronomique néo-Calédonien (IAC), BP 73 Port Laguerre, 98890 Païta, New Caledonia.
[Ti] Título:The morphophysiological dormancy in Amborella trichopoda seeds is a pleisiomorphic trait in angiosperms.
[So] Source:Ann Bot;119(4):581-590, 2017 Mar 01.
[Is] ISSN:1095-8290
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Background and Aims: Recent parsimony-based reconstructions suggest that seeds of early angiosperms had either morphophysiological or physiological dormancy, with the former considered as more probable. The aim of this study was to determine the class of seed dormancy present in Amborella trichopoda , the sole living representative of the most basal angiosperm lineage Amborellales, with a view to resolving fully the class of dormancy present at the base of the angiosperm clade. Methods: Drupes of A. trichopoda without fleshy parts were germinated and dissected to observe their structure and embryo growth. Pre-treatments including acid scarification, gibberellin treatment and seed excision were tested to determine their influence on dormancy breakage and germination. Character-state mapping by maximum parsimony, incorporating data from the present work and published sources, was then used to determine the likely class of dormancy present in early angiosperms. Key Results: Germination in A. trichopoda requires a warm stratification period of at least approx. 90 d, which is followed by endosperm swelling, causing the water-permeable pericarp-mesocarp envelope to split open. The embryo then grows rapidly within the seed, to radicle emergence some 17 d later and cotyledon emergence after an additional 24 d. Gibberellin treatment, acid scarification and excision of seeds from the surrounding drupe tissues all promoted germination by shortening the initial phase of dormancy, prior to embryo growth. Conclusions: Seeds of A. trichopoda have non-deep simple morphophysiological dormancy, in which mechanical resistance of the pericarp-mesocarp envelope plays a key role in the initial physiological phase. Maximum parsimony analyses, including data obtained in the present work, indicate that morphophysiological dormancy is likely to be a pleisiomorphic trait in flowering plants. The significance of this conclusion for studies of early angiosperm evolution is discussed.
[Mh] Termos MeSH primário: Magnoliopsida/fisiologia
Dormência de Plantas/fisiologia
Sementes/fisiologia
[Mh] Termos MeSH secundário: Evolução Biológica
Germinação/fisiologia
Magnoliopsida/anatomia & histologia
Magnoliopsida/ultraestrutura
Microscopia Eletrônica de Varredura
Sementes/anatomia & histologia
Sementes/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170115
[St] Status:MEDLINE
[do] DOI:10.1093/aob/mcw244



página 1 de 43 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde