Base de dados : MEDLINE
Pesquisa : G07.345.750 [Categoria DeCS]
Referências encontradas : 579 [refinar]
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  1 / 579 MEDLINE  
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[PMID]:28985225
[Au] Autor:Parkyn Schneider M; Liu B; Glock P; Suttie A; McHugh E; Andrew D; Batinovic S; Williamson N; Hanssen E; McMillan P; Hliscs M; Tilley L; Dixon MWA
[Ad] Endereço:Department of Biochemistry and Molecular Biology, The University of Melbourne, Melbourne, Victoria, Australia.
[Ti] Título:Disrupting assembly of the inner membrane complex blocks Plasmodium falciparum sexual stage development.
[So] Source:PLoS Pathog;13(10):e1006659, 2017 Oct.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transmission of malaria parasites relies on the formation of a specialized blood form called the gametocyte. Gametocytes of the human pathogen, Plasmodium falciparum, adopt a crescent shape. Their dramatic morphogenesis is driven by the assembly of a network of microtubules and an underpinning inner membrane complex (IMC). Using super-resolution optical and electron microscopies we define the ultrastructure of the IMC at different stages of gametocyte development. We characterize two new proteins of the gametocyte IMC, called PhIL1 and PIP1. Genetic disruption of PhIL1 or PIP1 ablates elongation and prevents formation of transmission-ready mature gametocytes. The maturation defect is accompanied by failure to form an enveloping IMC and a marked swelling of the digestive vacuole, suggesting PhIL1 and PIP1 are required for correct membrane trafficking. Using immunoprecipitation and mass spectrometry we reveal that PhIL1 interacts with known and new components of the gametocyte IMC.
[Mh] Termos MeSH primário: Microtúbulos/metabolismo
Plasmodium falciparum/crescimento & desenvolvimento
Desenvolvimento Sexual/fisiologia
[Mh] Termos MeSH secundário: Animais
Microscopia Eletrônica/métodos
Microtúbulos/ultraestrutura
Plasmodium falciparum/ultraestrutura
Transporte Proteico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006659


  2 / 579 MEDLINE  
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[PMID]:28949961
[Au] Autor:Kuroki S; Okashita N; Baba S; Maeda R; Miyawaki S; Yano M; Yamaguchi M; Kitano S; Miyachi H; Itoh A; Yoshida M; Tachibana M
[Ad] Endereço:Division of Epigenome Dynamics, Institute of Advanced Medical Sciences, Tokushima University, Tokushima, Japan.
[Ti] Título:Rescuing the aberrant sex development of H3K9 demethylase Jmjd1a-deficient mice by modulating H3K9 methylation balance.
[So] Source:PLoS Genet;13(9):e1007034, 2017 Sep.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Histone H3 lysine 9 (H3K9) methylation is a hallmark of heterochromatin. H3K9 demethylation is crucial in mouse sex determination; The H3K9 demethylase Jmjd1a deficiency leads to increased H3K9 methylation at the Sry locus in embryonic gonads, thereby compromising Sry expression and causing male-to-female sex reversal. We hypothesized that the H3K9 methylation level at the Sry locus is finely tuned by the balance in activities between the H3K9 demethylase Jmjd1a and an unidentified H3K9 methyltransferase to ensure correct Sry expression. Here we identified the GLP/G9a H3K9 methyltransferase complex as the enzyme catalyzing H3K9 methylation at the Sry locus. Based on this finding, we tried to rescue the sex-reversal phenotype of Jmjd1a-deficient mice by modulating GLP/G9a complex activity. A heterozygous GLP mutation rescued the sex-reversal phenotype of Jmjd1a-deficient mice by restoring Sry expression. The administration of a chemical inhibitor of GLP/G9a enzyme into Jmjd1a-deficient embryos also successfully rescued sex reversal. Our study not only reveals the molecular mechanism underlying the tuning of Sry expression but also provides proof on the principle of therapeutic strategies based on the pharmacological modulation of epigenetic balance.
[Mh] Termos MeSH primário: Histona-Lisina N-Metiltransferase/metabolismo
Histonas/metabolismo
Histona Desmetilases com o Domínio Jumonji/genética
Proteína da Região Y Determinante do Sexo/metabolismo
Desenvolvimento Sexual/genética
[Mh] Termos MeSH secundário: Animais
Feminino
Regulação da Expressão Gênica
Loci Gênicos
Gônadas/embriologia
Gônadas/metabolismo
Histona-Lisina N-Metiltransferase/genética
Histonas/genética
Histona Desmetilases com o Domínio Jumonji/deficiência
Histona Desmetilases com o Domínio Jumonji/metabolismo
Masculino
Metilação
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Fatores de Transcrição SOX9/genética
Fatores de Transcrição SOX9/metabolismo
Análise de Sequência de DNA
Proteína da Região Y Determinante do Sexo/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Histones); 0 (SOX9 Transcription Factor); 0 (Sex-Determining Region Y Protein); 0 (Sox9 protein, mouse); 0 (Sry protein, mouse); EC 1.14.11.- (Jumonji Domain-Containing Histone Demethylases); EC 1.14.11.- (Kdm3a protein, mouse); EC 2.1.1.43 (G9a protein, mouse); EC 2.1.1.43 (GLP protein, mouse); EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171022
[Lr] Data última revisão:
171022
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007034


  3 / 579 MEDLINE  
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[PMID]:28895116
[Au] Autor:Pittet F; Johnson C; Hinde K
[Ad] Endereço:Brain, Mind, and Behavior Unit, California National Primate Research Center, Davis, California.
[Ti] Título:Age at reproductive debut: Developmental predictors and consequences for lactation, infant mass, and subsequent reproduction in rhesus macaques (Macaca mulatta).
[So] Source:Am J Phys Anthropol;164(3):457-476, 2017 Nov.
[Is] ISSN:1096-8644
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The age at which females initiate their reproductive career is a critical life-history parameter with potential consequences on their residual reproductive value and lifetime fitness. The age at reproductive debut may be intimately tied to the somatic capacity of the mother to rear her young, but relatively little is known about the influence of age of first birth on milk synthesis within a broader framework of reproductive scheduling, infant outcomes, and other life-history tradeoffs. MATERIAL AND METHODS: Our study investigated the predictors of age at first reproduction among 108 captive rhesus macaque (Macaca mulatta) females, and associations with their milk synthesis at peak lactation, infant mass, and ability to subsequently conceive and reproduce. RESULTS: The majority of females reproduced in their fourth year (typical breeders); far fewer initiated their reproductive career one year earlier or one year later (respectively early and late breeders). Early breeders (3-year-old) benefited from highly favorable early life development (better juvenile growth, high dominance rank) to accelerate reproduction, but were impaired in milk synthesis due to lower somatic resources and their own continued growth. Comparatively, late breeders suffered from poor developmental conditions, only partially compensated by their delayed reproduction, and evinced compromised milk synthesis. Typical breeders not only produced higher available milk energy but also had best reproductive performance during the breeding and birth seasons following primiparity. DISCUSSION: Here, we refine and extend our understanding of how life-history tradeoffs manifest in the magnitude, sources, and consequences of variation in age of reproductive debut. These findings provide insight into primate reproductive flexibility in the context of constraints and opportunities.
[Mh] Termos MeSH primário: Macaca mulatta/crescimento & desenvolvimento
Macaca mulatta/fisiologia
Comportamento Sexual Animal/fisiologia
Desenvolvimento Sexual/fisiologia
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Animais Recém-Nascidos/fisiologia
Tamanho Corporal/fisiologia
Feminino
Lactação/fisiologia
Leite/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1002/ajpa.23286


  4 / 579 MEDLINE  
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[PMID]:28533820
[Au] Autor:Radhakrishnan S; Literman R; Mizoguchi B; Valenzuela N
[Ad] Endereço:Bioinformatics and Computational Biology Program, Iowa State University, Ames, IA 50011 USA.
[Ti] Título:MeDIP-seq and nCpG analyses illuminate sexually dimorphic methylation of gonadal development genes with high historic methylation in turtle hatchlings with temperature-dependent sex determination.
[So] Source:Epigenetics Chromatin;10:28, 2017.
[Is] ISSN:1756-8935
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: DNA methylation alters gene expression but not DNA sequence and mediates some cases of phenotypic plasticity. Temperature-dependent sex determination (TSD) epitomizes phenotypic plasticity where environmental temperature drives embryonic sexual fate, as occurs commonly in turtles. Importantly, the temperature-specific transcription of two genes underlying gonadal differentiation is known to be induced by differential methylation in TSD fish, turtle and alligator. Yet, how extensive is the link between DNA methylation and TSD remains unclear. Here we test for broad differences in genome-wide DNA methylation between male and female hatchling gonads of the TSD painted turtle using methyl DNA immunoprecipitation sequencing, to identify differentially methylated candidates for future study. We also examine the genome-wide nCpG distribution (which affects DNA methylation) in painted turtles and test for historic methylation in genes regulating vertebrate gonadogenesis. RESULTS: Turtle global methylation was consistent with other vertebrates (57% of the genome, 78% of all CpG dinucleotides). Numerous genes predicted to regulate turtle gonadogenesis exhibited sex-specific methylation and were proximal to methylated repeats. nCpG distribution predicted actual turtle DNA methylation and was bimodal in gene promoters (as other vertebrates) and introns (unlike other vertebrates). Differentially methylated genes, including regulators of sexual development, had lower nCpG content indicative of higher historic methylation. CONCLUSIONS: Ours is the first evidence suggesting that sexually dimorphic DNA methylation is pervasive in turtle gonads (perhaps mediated by repeat methylation) and that it targets numerous regulators of gonadal development, consistent with the hypothesis that it may regulate thermosensitive transcription in TSD vertebrates. However, further research during embryogenesis will help test this hypothesis and the alternative that instead, most differential methylation observed in hatchlings is the by-product of sexual differentiation and not its cause.
[Mh] Termos MeSH primário: Metilação de DNA/genética
Gônadas/crescimento & desenvolvimento
Processos de Determinação Sexual
Tartarugas/genética
[Mh] Termos MeSH secundário: Animais
Embrião não Mamífero
Desenvolvimento Embrionário
Feminino
Regulação da Expressão Gênica no Desenvolvimento
Gônadas/metabolismo
Masculino
Desenvolvimento Sexual
Temperatura Ambiente
Tartarugas/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE
[do] DOI:10.1186/s13072-017-0136-2


  5 / 579 MEDLINE  
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[PMID]:28338982
[Au] Autor:Teichert I; Dahlmann TA; Kück U; Nowrousian M
[Ad] Endereço:Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-Universität Bochum, Germany.
[Ti] Título:RNA Editing During Sexual Development Occurs in Distantly Related Filamentous Ascomycetes.
[So] Source:Genome Biol Evol;9(4):855-868, 2017 Apr 01.
[Is] ISSN:1759-6653
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:RNA editing is a post-transcriptional process that modifies RNA molecules leading to transcript sequences that differ from their template DNA. A-to-I editing was found to be widely distributed in nuclear transcripts of metazoa, but was detected in fungi only recently in a study of the filamentous ascomycete Fusarium graminearum that revealed extensive A-to-I editing of mRNAs in sexual structures (fruiting bodies). Here, we searched for putative RNA editing events in RNA-seq data from Sordaria macrospora and Pyronema confluens, two distantly related filamentous ascomycetes, and in data from the Taphrinomycete Schizosaccharomyces pombe. Like F. graminearum, S. macrospora is a member of the Sordariomycetes, whereas P. confluens belongs to the early-diverging group of Pezizomycetes. We found extensive A-to-I editing in RNA-seq data from sexual mycelium from both filamentous ascomycetes, but not in vegetative structures. A-to-I editing was not detected in different stages of meiosis of S. pombe. A comparison of A-to-I editing in S. macrospora with F. graminearum and P. confluens, respectively, revealed little conservation of individual editing sites. An analysis of RNA-seq data from two sterile developmental mutants of S. macrospora showed that A-to-I editing is strongly reduced in these strains. Sequencing of cDNA fragments containing more than one editing site from P. confluens showed that at the beginning of sexual development, transcripts were incompletely edited or unedited, whereas in later stages transcripts were more extensively edited. Taken together, these data suggest that A-to-I RNA editing is an evolutionary conserved feature during fruiting body development in filamentous ascomycetes.
[Mh] Termos MeSH primário: Ascomicetos/genética
Fungos/genética
Edição de RNA/genética
Desenvolvimento Sexual/genética
[Mh] Termos MeSH secundário: Ascomicetos/crescimento & desenvolvimento
Sequência de Bases/genética
Carpóforos/genética
Carpóforos/crescimento & desenvolvimento
Fungos/crescimento & desenvolvimento
Fusarium/genética
Fusarium/crescimento & desenvolvimento
Regulação Fúngica da Expressão Gênica
Mutação
Schizosaccharomyces/genética
Schizosaccharomyces/crescimento & desenvolvimento
Sordariales/genética
Sordariales/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1093/gbe/evx052


  6 / 579 MEDLINE  
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[PMID]:28237997
[Au] Autor:Yun YH; Oh MH; Kim JY; Kim SH
[Ad] Endereço:Department of Microbiology, Dankook University, Cheonan 31116, Republic of Korea.
[Ti] Título:, a Hybrid Histidine Kinase Gene, Is Essential for the Sexual Development and Virulence of .
[So] Source:J Microbiol Biotechnol;27(5):1010-1022, 2017 May 28.
[Is] ISSN:1738-8872
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Hybrid histidine kinase is part of a two-component system that is required for various stress responses and pathogenesis of pathogenic fungi. The gene in human pathogen encodes a hybrid histidine kinase and is important for pathogenesis. In this study, we identified a homolog, , in the maize pathogen by bioinformatics analysis. To explore the role of in the survival of under environmental stresses and its pathogenesis, mutants were constructed by allelic exchange. The growth of mutants was significantly impaired when they were cultured under hyperosmotic stress. The mutants exhibited increased resistance to antifungal agent fludioxonil. In particular, the mutants were unable to produce cytokinesis or conjugation tubes, and to develop fuzzy filaments, resulting in impaired mating between compatible strains. The expression levels of , and , which are involved in the pheromone pathway, were significantly decreased in the mutants. In inoculation tests to the host plant, the mutants showed significantly reduced ability in the production of anthocyanin pigments and tumor development on maize leaves. Overall, the combined results indicated that plays important roles in the survival under hyperosmotic stress, and contributes to cytokinesis, sexual development, and virulence of by regulating the expression of the genes involved in the pheromone pathway.
[Mh] Termos MeSH primário: Genes Fúngicos Tipo Acasalamento/genética
Histidina Quinase/genética
Desenvolvimento Sexual/genética
Ustilago/crescimento & desenvolvimento
Ustilago/patogenicidade
Virulência/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Antocianinas/metabolismo
Antifúngicos/farmacologia
Cultura Axênica
Biologia Computacional
Citocinese
DNA Fúngico/genética
Dioxóis/farmacologia
Escherichia coli/genética
Proteínas Fúngicas/metabolismo
Regulação Fúngica da Expressão Gênica
Proteínas de Grupo de Alta Mobilidade/metabolismo
Histidina Quinase/classificação
Hiperostose
Mutação
Pressão Osmótica
Fenótipo
Feromônios/metabolismo
Filogenia
Doenças das Plantas/microbiologia
Folhas de Planta/metabolismo
Proteínas de Plantas/metabolismo
Pirróis/farmacologia
RNA Mensageiro/análise
Receptores de Feromonas/metabolismo
Alinhamento de Sequência
Estresse Fisiológico/genética
Fatores de Transcrição/metabolismo
Ustilago/efeitos dos fármacos
Zea mays/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antifungal Agents); 0 (DNA, Fungal); 0 (Dioxoles); 0 (Fungal Proteins); 0 (High Mobility Group Proteins); 0 (Pheromones); 0 (Plant Proteins); 0 (Pyrroles); 0 (RNA, Messenger); 0 (Receptors, Pheromone); 0 (Transcription Factors); 0 (pheromone response factor 1, Ustilago maydis); EC 2.7.13.1 (Histidine Kinase); ENS9J0YM16 (fludioxonil)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE
[do] DOI:10.4014/jmb.1702.02001


  7 / 579 MEDLINE  
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[PMID]:28127758
[Au] Autor:Alhomaidah D; McGowan R; Ahmed SF
[Ad] Endereço:Developmental Endocrinology Research Group, University of Glasgow, Glasgow, UK.
[Ti] Título:The current state of diagnostic genetics for conditions affecting sex development.
[So] Source:Clin Genet;91(2):157-162, 2017 Feb.
[Is] ISSN:1399-0004
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Disorders of sex development (DSD), are a group of rare congenital conditions. Unlike 46, XX DSD where the cause is usually clear, identification of a cause of XY DSD is often unclear and may be attributed to a disorder of gonadal development, androgen synthesis or androgen action. Reaching a firm diagnosis is challenging and requires expertise within a framework that abides by the highest standards of clinical care. Whilst conditions associated with altered sex development have improved our fundamental understanding of sex and gonadal development, it is debatable whether this improvement in our understanding has improved the lives of people with DSD. Thus, there is a need for more emphasis on showing that a firm diagnosis for conditions associated with DSD is associated with a change in clinical practice that benefits the patient. With the rapid advances in diagnostic technology, there is also a need for clearer guidance on the relative merits of biochemical vs genetic evaluation. The standardization and harmonization of complex genetic and biochemical analyses for rare conditions are issues that require further guidance and it is probably that international networks and registries for rare conditions will facilitate the development of this framework.
[Mh] Termos MeSH primário: Transtornos do Desenvolvimento Sexual/diagnóstico
Transtornos do Desenvolvimento Sexual/genética
Gônadas/crescimento & desenvolvimento
Desenvolvimento Sexual/genética
[Mh] Termos MeSH secundário: Androgênios/genética
Transtornos do Desenvolvimento Sexual/patologia
Feminino
Seres Humanos
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Androgens)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE
[do] DOI:10.1111/cge.12912


  8 / 579 MEDLINE  
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[PMID]:28089630
[Au] Autor:Soukup AA; Fischer GJ; Luo J; Keller NP
[Ad] Endereço:Department of Genetics, University of Wisconsin-Madison, WI, United States.
[Ti] Título:The Aspergillus nidulans Pbp1 homolog is required for normal sexual development and secondary metabolism.
[So] Source:Fungal Genet Biol;100:13-21, 2017 Mar.
[Is] ISSN:1096-0937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:P bodies and stress granules are RNA-containing structures governing mRNA degradation and translational arrest, respectively. Saccharomyces cerevisiae Pbp1 protein localizes to stress granules and promotes their formation and is involved in proper polyadenylation, suppression of RNA-DNA hybrids, and preventing aberrant rDNA recombination. A genetic screen for Aspergillus nidulans mutants aberrant in secondary metabolism identified the Pbp1 homolog, PbpA. Using Dcp1 (mRNA decapping) as a marker for P-body formation and FabM (Pab1, poly-A binding protein) to track stress granule accumulation, we examine the dynamics of RNA granule formation in A. nidulans cells lacking pub1, edc3, and pbpA. Although PbpA acts as a functional homolog of yeast PBP1, PbpA had little impact on either P-body or stress granule formation in A. nidulans in contrast to Pub1 and Edc3. However, we find that PbpA is critical for sexual development and its loss increases the production of some secondary metabolites including the carcinogen sterigmatocystin.
[Mh] Termos MeSH primário: Aspergillus nidulans/genética
Proteínas de Transporte/genética
Proteínas de Saccharomyces cerevisiae/genética
Metabolismo Secundário/genética
Desenvolvimento Sexual/genética
[Mh] Termos MeSH secundário: Grânulos Citoplasmáticos/metabolismo
Ligação Proteica
Biossíntese de Proteínas
Estabilidade de RNA/genética
Saccharomyces cerevisiae/genética
Esterigmatocistina/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (PBP1 protein, S cerevisiae); 0 (Saccharomyces cerevisiae Proteins); 10048-13-2 (Sterigmatocystin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170721
[Lr] Data última revisão:
170721
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170117
[St] Status:MEDLINE


  9 / 579 MEDLINE  
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[PMID]:27940159
[Au] Autor:Webster KA; Schach U; Ordaz A; Steinfeld JS; Draper BW; Siegfried KR
[Ad] Endereço:University of Massachusetts Boston, Biology Department, 100 Morrissey Blvd., Boston, MA 02125, USA.
[Ti] Título:Dmrt1 is necessary for male sexual development in zebrafish.
[So] Source:Dev Biol;422(1):33-46, 2017 02 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The dmrt1 (doublesex and mab-3 related transcription factor 1) gene is a key regulator of sex determination and/or gonadal sex differentiation across metazoan animals. This is unusual given that sex determination genes are typically not well conserved. The mechanisms by which zebrafish sex is determined have remained elusive due to the lack of sex chromosomes and the complex polygenic nature of sex determination in domesticated strains. To investigate the role of dmrt1 in zebrafish sex determination and gonad development, we isolated mutations disrupting this gene. We found that the majority of dmrt1 mutant fish develop as fertile females suggesting a complete male-to-female sex reversal in mutant animals that would have otherwise developed as males. A small percentage of mutant animals became males, but were sterile and displayed testicular dysgenesis. Therefore zebrafish dmrt1 functions in male sex determination and testis development. Mutant males had aberrant gonadal development at the onset of gonadal sex-differentiation, displaying reduced oocyte apoptosis followed by development of intersex gonads and failed testis morphogenesis and spermatogenesis. By contrast, female ovaries developed normally. We found that Dmrt1 is necessary for normal transcriptional regulation of the amh (anti-Müllerian hormone) and foxl2 (forkhead box L2) genes, which are thought to be important for male or female sexual development respectively. Interestingly, we identified one dmrt1 mutant allele that co-operates with a linked segregation distorter locus to generate an apparent XY sex determination mechanism. We conclude that dmrt1 is dispensable for ovary development but necessary for testis development in zebrafish, and that dmrt1 promotes male development by transcriptionally regulating male and female genes as has been described in other animals. Furthermore, the strong sex-ratio bias caused by dmrt1 reduction-of-function points to potential mechanisms through which sex chromosomes may evolve.
[Mh] Termos MeSH primário: Desenvolvimento Sexual
Testículo/embriologia
Fatores de Transcrição/fisiologia
Peixe-Zebra/embriologia
[Mh] Termos MeSH secundário: Animais
Feminino
Proteína Forkhead Box L2
Fatores de Transcrição Forkhead/análise
Regulação da Expressão Gênica no Desenvolvimento
Masculino
Cromossomos Sexuais
Processos de Determinação Sexual
Diferenciação Sexual
Fatores de Transcrição/genética
Proteínas de Peixe-Zebra/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DMRT1 protein); 0 (Forkhead Box Protein L2); 0 (Forkhead Transcription Factors); 0 (Foxl2 protein, zebrafish); 0 (Transcription Factors); 0 (Zebrafish Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


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[PMID]:27785716
[Au] Autor:Park CB; Kim YJ; Soyano K
[Ad] Endereço:Environmental Safety Group, Korea Institute of Science and Technology Europe, 66123, Saarbruecken, Germany. cb.park@kist-europe.de.
[Ti] Título:Effects of increasing temperature due to aquatic climate change on the self-fertility and the sexual development of the hermaphrodite fish, Kryptolebias marmoratus.
[So] Source:Environ Sci Pollut Res Int;24(2):1484-1494, 2017 Jan.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In order to assess the effects of increasing temperature on the reproductive performance of fish, different thermal conditions (i.e., 25.0, 26.5, 27.5, 28.5, 30.0 °C) were used in this study and the self-fertilizing hermaphrodite fish, Kryptolebias marmoratus, was exposed to these different thermal conditions. During an exposure period of 30 to 150 days, the gonadosomatic index (GSI), gonadal development, the levels of plasma 17ß-estradial (E2) and testosterone (T), hepatic vitellogenin (VTG) mRNA abundance, and the number of self-fertilized eggs were analyzed. This study confirmed that a high water temperature above 27.5 °C led to the suppression of self-fertility of hermaphroditic fish from 30 days after exposure. The oocyte quality and maturation would be affected by the disruption of hepatic VTG synthesis at a high water temperature of 30 °C, which resulted in the reduced the self-fertility in K. marmoratus. Consequently, this study suggests that elevated water temperature due to aquatic climate change prior to sexual maturation and the onset of spawning can lead to the reproductive dysfunction of hermaphroditic K. marmoratus.
[Mh] Termos MeSH primário: Mudança Climática
Fertilidade
Temperatura Alta
Peixes Listrados/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Gônadas/crescimento & desenvolvimento
Masculino
Reprodução
Autofertilização
Desenvolvimento Sexual
Maturidade Sexual
Vitelogeninas/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Vitellogenins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161028
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-016-7878-4



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