Base de dados : MEDLINE
Pesquisa : G07.775.249 [Categoria DeCS]
Referências encontradas : 434 [refinar]
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[PMID]:28470617
[Au] Autor:Sugiki T; Fujiwara T; Kojima C
[Ad] Endereço:Graduate School of Engineering, Yokohama National University, Yokohama, Japan.
[Ti] Título:Cold-Shock Expression System in E. coli for Protein NMR Studies.
[So] Source:Methods Mol Biol;1586:345-357, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The cold-shock system using the pCold vector is one of the most effective Escherichia coli heterologous protein expression systems. It allows the improvement of the expression level of the protein of interest in a soluble fraction. In this chapter, we describe practical procedures for the overexpression of heterologous protein of interest by using the pCold vector or the single-protein production system. The latter is one of the most advanced pCold technologies for isotope labeling of the target protein and its NMR studies.
[Mh] Termos MeSH primário: Clonagem Molecular/métodos
Resposta ao Choque Frio
Escherichia coli/genética
Vetores Genéticos/genética
[Mh] Termos MeSH secundário: Técnicas de Cultura de Células/métodos
Temperatura Baixa
Escherichia coli/fisiologia
Marcação por Isótopo/métodos
Ressonância Magnética Nuclear Biomolecular/métodos
Proteínas Recombinantes/genética
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6887-9_23


  2 / 434 MEDLINE  
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[PMID]:29175331
[Au] Autor:Fujita T; Liu Y; Higashitsuji H; Itoh K; Shibasaki K; Fujita J; Nishiyama H
[Ad] Endereço:Department of Urology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan; Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
[Ti] Título:Involvement of TRPV3 and TRPM8 ion channel proteins in induction of mammalian cold-inducible proteins.
[So] Source:Biochem Biophys Res Commun;495(1):935-940, 2018 01 01.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cold-inducible RNA-binding protein (CIRP), RNA-binding motif protein 3 (RBM3) and serine and arginine rich splicing factor 5 (SRSF5) are RNA-binding proteins that are transcriptionally upregulated in response to moderately low temperatures and a variety of cellular stresses in mammalian cells. Induction of these cold-inducible proteins (CIPs) is dependent on transient receptor potential (TRP) V4 channel protein, but seems independent of its ion channel activity. We herein report that in addition to TRPV4, TRPV3 and TRPM8 are necessary for the induction of CIPs. We established cell lines from the lung of TRPV4-knockout (KO) mouse, and observed induction of CIPs in them by western blot analysis. A TRPV4 antagonist RN1734 suppressed the induction in wild-type mouse cells, but not in TRPV4-KO cells. A TRPV3 channel blocker S408271 and a TRPM8 channel blocker AMTB as well as siRNAs against TRPV3 and TRPM8 suppressed the CIP induction in mouse TRPV4-KO cells and human U-2 OS cells. A TRPV3 channel agonist 2-APB induced CIP expression, but camphor did not. Neither did a TRPM8 channel agonist WS-12. These results suggest that TRPV4, TRPV3 and TRPM8 proteins, but not their ion channel activities are necessary for the induction of CIPs at 32 °C. Identification of proteins that differentially interact with these TRP channels at 37 °C and 32 °C would help elucidate the underlying mechanisms of CIP induction by hypothermia.
[Mh] Termos MeSH primário: Proteínas e Peptídeos de Choque Frio/metabolismo
Resposta ao Choque Frio/fisiologia
Ativação do Canal Iônico/fisiologia
Canais de Cátion TRPM/metabolismo
Canais de Cátion TRPV/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Temperatura Baixa
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cold Shock Proteins and Peptides); 0 (TRPM Cation Channels); 0 (TRPM8 protein, mouse); 0 (TRPV Cation Channels); 0 (Trpv3 protein, mouse); 0 (Trpv4 protein, mouse)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


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[PMID]:29175325
[Au] Autor:Zou S; Lang T; Zhang B; Huang K; Gong L; Luo H; Xu W; He X
[Ad] Endereço:Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China; Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural
[Ti] Título:Fatty acid oxidation alleviates the energy deficiency caused by the loss of MPC1 in MPC1 mice.
[So] Source:Biochem Biophys Res Commun;495(1):1008-1013, 2018 01 01.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pyruvate is a central substrate in energy metabolism, paramount to carbohydrate, fat, and amino acid catabolic and anabolic pathways. Mitochondrial pyruvate carrier 1(MPC1) is one important component of the complex that facilitates mitochondrial pyruvate import. Complete MPC1 deficiency is a serious concern, and has been shown to result in embryonic lethality in mice. The study outlined in this paper generated one mouse line with the MPC1 protein part deficiency by using the CRISPR/Cas9 system. Clinical observations, body weight and organ/tissue weight, gas exchange, cold-stimulation, blood parameters, as well as histopathology analysis were analyzed to evaluate potential physiological abnormalities caused by MPC1 deficiency. Results indicate that MPC1 mice experienced a change in important clinical criteria such as low body weight, decreased movement, and low body shell temperature, few adipose accumulate. The mice show significant difference in some blood parameters including apo-B100, apo-A1, HDL, glucagon, insulin. However these changes alleviated while being fed with the HFD, which provided metabolites to sustain the TCA cycle and body development. The MPC1 mice may employ fatty acid oxidation to meet their bioenergetic demands. This study suggests that inhibition of MPC1 activity can boost fatty acid oxidation to provide sufficient energy to the body. This work promotes further studies regarding the interplay between carbohydrate and fat metabolism.
[Mh] Termos MeSH primário: Peso Corporal/fisiologia
Metabolismo Energético/fisiologia
Ácidos Graxos/metabolismo
Consumo de Oxigênio/fisiologia
Pró-Proteína Convertase 1/metabolismo
Ácido Pirúvico/metabolismo
[Mh] Termos MeSH secundário: Animais
Resposta ao Choque Frio/fisiologia
Ativação Enzimática
Masculino
Camundongos
Camundongos Knockout
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fatty Acids); 8558G7RUTR (Pyruvic Acid); EC 3.4.21.93 (Pcsk1 protein, mouse); EC 3.4.21.93 (Proprotein Convertase 1)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


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[PMID]:29023475
[Au] Autor:Wang D; Li L; Wu G; Vasseur L; Yang G; Huang P
[Ad] Endereço:Tea Research Institute, Fujian Academy of Agricultural Sciences, Fu'an, Fujian, China.
[Ti] Título:De novo transcriptome sequencing of Isaria cateniannulata and comparative analysis of gene expression in response to heat and cold stresses.
[So] Source:PLoS One;12(10):e0186040, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Isaria cateniannulata is a very important and virulent entomopathogenic fungus that infects many insect pest species. Although I. cateniannulata is commonly exposed to extreme environmental temperature conditions, little is known about its molecular response mechanism to temperature stress. Here, we sequenced and de novo assembled the transcriptome of I. cateniannulata in response to high and low temperature stresses using Illumina RNA-Seq technology. Our assembly encompassed 17,514 unigenes (mean length = 1,197 bp), in which 11,445 unigenes (65.34%) showed significant similarities to known sequences in NCBI non-redundant protein sequences (Nr) database. Using digital gene expression analysis, 4,483 differentially expressed genes (DEGs) were identified after heat treatment, including 2,905 up-regulated genes and 1,578 down-regulated genes. Under cold stress, 1,927 DEGs were identified, including 1,245 up-regulated genes and 682 down-regulated genes. The expression patterns of 18 randomly selected candidate DEGs resulting from quantitative real-time PCR (qRT-PCR) were consistent with their transcriptome analysis results. Although DEGs were involved in many pathways, we focused on the genes that were involved in endocytosis: In heat stress, the pathway of clathrin-dependent endocytosis (CDE) was active; however at low temperature stresses, the pathway of clathrin-independent endocytosis (CIE) was active. Besides, four categories of DEGs acting as temperature sensors were observed, including cell-wall-major-components-metabolism-related (CWMCMR) genes, heat shock protein (Hsp) genes, intracellular-compatible-solutes-metabolism-related (ICSMR) genes and glutathione S-transferase (GST). These results enhance our understanding of the molecular mechanisms of I. cateniannulata in response to temperature stresses and provide a valuable resource for the future investigations.
[Mh] Termos MeSH primário: Resposta ao Choque Frio/genética
Regulação Fúngica da Expressão Gênica
Resposta ao Choque Térmico/genética
Hypocreales/fisiologia
[Mh] Termos MeSH secundário: Perfilação da Expressão Gênica
Ontologia Genética
Hypocreales/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186040


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[PMID]:28912063
[Au] Autor:Liu L; Song Y; Xu J; Li D; Li G; An L
[Ad] Endereço:Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
[Ti] Título:Differential expression by chromatin modifications of alcohol dehydrogenase 1 of Chorispora bungeana in cold stress.
[So] Source:Gene;636:1-16, 2017 Dec 15.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Epigenetic modifications regulate plant genes to cope with a variety of environmental stresses. Chorispora bungeana is an alpine subnival plant with strong tolerance to multiple abiotic stresses, especially cold stress. In this study, we characterized the alcohol dehydrogenase 1 gene from Chorispora bungeana, CbADH1, that is up-regulated in cold conditions. Overexpression of CbADH1 in Arabidopsis thaliana improved cold tolerance, as indicated by a decreased lethal temperature (LT50). Chromatin immunoprecipitation assays showed that histone H3 is removed from the promoter region and the middle-coding region of the gene. H3K9 acetylation and H3K4 trimethylation increased throughout the gene and in the proximal promoter region, respectively. Moreover, increased Ser5P and Ser2P polymerase II accumulation further indicated changes in the transcription initiation and elongation of CbADH1 were due to the cold stress. Taken together, our results suggested that CbADH1 is highly expressed during cold stress, and is regulated by epigenetic modifications. This study expands our understanding of the regulation of gene expression by epigenetic modifications in response to environmental cues.
[Mh] Termos MeSH primário: Álcool Desidrogenase/genética
Brassicaceae/enzimologia
Resposta ao Choque Frio/genética
Proteínas de Plantas/genética
[Mh] Termos MeSH secundário: Álcool Desidrogenase/química
Álcool Desidrogenase/metabolismo
Sequência de Aminoácidos
Arabidopsis/genética
Brassicaceae/genética
Cromatina/metabolismo
Clonagem Molecular
Sequência Conservada
Histonas/metabolismo
Folhas de Planta/enzimologia
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
RNA Polimerase II/metabolismo
Elementos de Resposta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (Histones); 0 (Plant Proteins); EC 1.1.1.1 (Alcohol Dehydrogenase); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE


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[PMID]:28671258
[Au] Autor:Auler PA; Benitez LC; do Amaral MN; Vighi IL; Rodrigues GS; da Maia LC; Braga EJB
[Ad] Endereço:Departamento de Botânica, Instituto de Biologia, , , Brasil pri_auler@hotmail.com.
[Ti] Título:Selection of candidate reference genes and validation for real-time PCR studies in rice plants exposed to low temperatures.
[So] Source:Genet Mol Res;16(2), 2017 Jun 29.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Rice is a cereal that presents a great ability to adapt to different soil and climate conditions. However, as it is a tropical crop with C3 metabolism, it performs better in warm temperatures with high solar radiation. Tolerance to stress caused by low temperatures is a highly complex process that involves various metabolic pathways and cellular compartments, resulting in general or specific effects on plant growth and development. In order to observe the true effect of a particular stress on genetic expression, reference genes need to be chosen for real-time PCRs, the expression levels of which should remain stable independent of the situation imposed. In this paper, the expression stability was evaluated of the actin 11 (ACT11), ubiquitin-conjugating enzyme 2 (UBC-E2), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), beta tubilin (ß-Tubulin), eukaryotic initiation factor 4α (eIF-4-α), eukaryotic initiation factor 1α (eIF-1-α), ubiquitin 10 (UBQ10), ubiquitin 5 (UBQ5), aquaporin (TIP41), and cyclophilin genes, in two rice genotypes cultivated in low temperature (13°C) conditions in vegetative stage (V4). The analysis material (leaves) was collected after 0, 6, 24, 48, and 72 h of exposure to the stress. In this study, the geNorm, BestKeeper, ΔCt, NormFinder, and RefFinder methods were used to evaluate the expression stability of the candidate reference genes. The results revealed that the most indicated genes for all the analysis methods were UBQ10 and UBQ5 for BRS Bojuru and BRS Pampa, respectively. On the other hand, the eIF-1-α gene presents the least expression stability and is not indicated for studies of rice plants subjected to low temperatures. The validation with the antioxidant system genes SODCc1-Cu/Zn, CATC, APX2, and GR2 confirmed the importance of using previously tested normalizing genes for adequate real-time PCR results.
[Mh] Termos MeSH primário: Resposta ao Choque Frio/genética
Genes de Plantas
Oryza/genética
Reação em Cadeia da Polimerase em Tempo Real/normas
[Mh] Termos MeSH secundário: Actinas/genética
Aquaporinas/genética
Ciclofilinas/genética
Fator de Iniciação 4A em Eucariotos/genética
Gliceraldeído-3-Fosfato Desidrogenase (Fosforiladora)/genética
Proteínas de Plantas/genética
Reação em Cadeia da Polimerase em Tempo Real/métodos
Padrões de Referência
Tubulina (Proteína)/genética
Ubiquitinas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Actins); 0 (Aquaporins); 0 (Plant Proteins); 0 (Tubulin); 0 (Ubiquitins); EC 1.2.1.12 (Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)); EC 2.7.7.- (Eukaryotic Initiation Factor-4A); EC 5.2.1.- (Cyclophilins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16029695


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[PMID]:28662501
[Au] Autor:Luo X; Jia R; Luo XQ; Wang G; Zhang QL; Qiao H; Wang N; Yan JQ
[Ad] Endereço:Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China.
[Ti] Título:Cold Exposure Differentially Stimulates Angiogenesis in BAT and WAT of Mice: Implication in Adrenergic Activation.
[So] Source:Cell Physiol Biochem;42(3):974-986, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: To characterize the temporal profile of cold-induced angiogenesis in brown and white adipose tissues of mice in vivo and the temporal changes of angiogenic factors in primary mice brown (BA) and white adipocytes (WA) treated with ß3-adrenoceptor agonist (CL316,243) in vitro. METHODS: 8-week old male C57BL/6J mice were individually housed in conventional cages under cold exposure (4°C) for 1, 2, 3, 4 and 5 days. Interscapular brown adipose tissue (iBAT), inguinal subcutaneous (sWAT) and epididymal white adipose tissues (eWAT) were harvested for immunohistochemical and gene expression analysis. In vitro, primary mice BA and WA treated with or without CL316,243 were harvested for gene expression and protein secretion analysis. RESULTS: A combination of morphological and genetic (Vegfa, Vegfr2, Hif-1α, Pai1 and Pedf) analyses demonstrated depot-specific angiogenesis in response to cold exposure. Upon CL316,243 treatment, angiogenic factors (Vegfa, Vegfr2, Hif-1α, Pai1 and Pedf) and secreted protein VEGFA were transiently increased in both BA and WA. CONCLUSION: Our results show that iBAT is highly responsive to cold-induced angiogenesis that is mainly supported by sWAT with a lesser extent by eWAT. Moreover, the angiogenesis is a transient process with the angiogenic factors may work in an autocrine/paracrine manner.
[Mh] Termos MeSH primário: Tecido Adiposo Marrom/irrigação sanguínea
Tecido Adiposo Marrom/fisiologia
Tecido Adiposo Branco/irrigação sanguínea
Tecido Adiposo Branco/fisiologia
Resposta ao Choque Frio
Neovascularização Fisiológica
[Mh] Termos MeSH secundário: Tecido Adiposo Marrom/citologia
Tecido Adiposo Branco/citologia
Animais
Células Cultivadas
Temperatura Baixa
Regulação da Expressão Gênica
Masculino
Camundongos Endogâmicos C57BL
Fator A de Crescimento do Endotélio Vascular/análise
Fator A de Crescimento do Endotélio Vascular/genética
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Vascular Endothelial Growth Factor A); 0 (vascular endothelial growth factor A, mouse)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE
[do] DOI:10.1159/000478680


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[PMID]:28656954
[Au] Autor:Shigeev VB; Shigeeev SV
[Ad] Endereço:Bureau of Forensic Medical Expertisem Moscow Health Department, Moscow, Russia, 107023.
[Ti] Título:[The forensic medical assessment of the causes and conditions of the formation of the chilling injury].
[Ti] Título:Sudebno-meditsinskaia otsenka prichin i uslovii vozniknoveniia kholodovoi travmy..
[So] Source:Sud Med Ekspert;60(3):42-49, 2017.
[Is] ISSN:0039-4521
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The authors present the data of the literature publications and theoretical considerations concerning the causes and conditions behind the formation of the chilling injury. It is demonstrated that the chilling injury develops as a consequence of a disturbance in the relationship between the hypothermic protection of the organism and the cooling potential of its environment. The thermal balance of the human organism depends not only on the natural mechanisms of physical and chemical thermoregulation but also on the character of artificial thermoregulation including the man-made means of cold protection. The critical evaluation of all the available data on chilling injuries to the human body gave evidence that the causes and conditions of their development can be highly multivarious which does not however exclude the possibility of their systematization.
[Mh] Termos MeSH primário: Temperatura Baixa/efeitos adversos
Congelamento das Extremidades
Hipotermia/patologia
[Mh] Termos MeSH secundário: Resposta ao Choque Frio
Medicina Legal/métodos
Congelamento das Extremidades/diagnóstico
Congelamento das Extremidades/etiologia
Congelamento das Extremidades/mortalidade
Congelamento das Extremidades/fisiopatologia
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.17116/sudmed201760342-49


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[PMID]:28653738
[Au] Autor:Xu Q; Wang YC; Liu R; Brito LF; Kang L; Yu Y; Wang DS; Wu HJ; Liu A
[Ad] Endereço:Xiertala Cattle Breeding Farm, Hailaer Farm Buro, Hailaer, Inner Mongolia, Moguai Farm, Hailaer Farm Buro, Hailaer, Inner Mongolia, China.
[Ti] Título:Differential gene expression in the peripheral blood of Chinese Sanhe cattle exposed to severe cold stress.
[So] Source:Genet Mol Res;16(2), 2017 Jun 20.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Livestock is an important food resource for the inhabitants of cold regions, such as northern Asia and alpine regions, where agriculture is limited. In these regions, cold stress largely affects livestock production, thereby reducing the productivity and survival of animals. Despite the importance of breeding cold-tolerant animals, few studies have investigated the effects of cold stress on cattle. Furthermore, whether severe cold stress alters gene expression or affects molecular genetic mechanisms remains unknown. Thus, we investigated gene expression changes in the peripheral blood samples of the Chinese Sanhe cattle exposed to severe cold. A total of 193 genes were found to exhibit significant alteration in expression (P < 0.05; fold change > 1.3), with 107 genes showing upregulation and 86 showing downregulation after cold exposure. The differences in the expression of 10 selected genes were further validated by real-time qRT-PCR. Further analyses showed that these differentially expressed genes (DEGs) were predominantly associated with important biological pathways and gene networks, such as lipid metabolism and cell death and survival, which are potentially associated with severe cold-stress resistance. Identification and description of these cold stress-induced DEGs might lead to the discovery of novel blood biomarkers that could be used to assess cold-stress resistance in cattle. To our knowledge, this is the first genomic evidence of differences in the transcript expression pattern in cattle exposed to severe cold stress. Our findings provide insights on the potential molecular mechanisms underlying cold-stress response in cattle.
[Mh] Termos MeSH primário: Resposta ao Choque Frio/genética
Redes Reguladoras de Genes
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Bovinos
Feminino
Perfilação da Expressão Gênica
Leucócitos
Análise de Sequência com Séries de Oligonucleotídeos
RNA Mensageiro
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16029593


  10 / 434 MEDLINE  
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[PMID]:28549208
[Au] Autor:Marini N; Bevilacqua CB; Büttow MV; Raseira MCB; Bonow S
[Ad] Endereço:Laboratório de Fisiologia e Tecnologia de Pós-Colheita, Embrapa Uva e Vinho, , Brasil.
[Ti] Título:Identification of reference genes for RT-qPCR analysis in peach genotypes with contrasting chilling requirements.
[So] Source:Genet Mol Res;16(2), 2017 May 25.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Selecting and validating reference genes are the first steps in studying gene expression by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). The present study aimed to evaluate the stability of five reference genes for the purpose of normalization when studying gene expression in various cultivars of Prunus persica with different chilling requirements. Flower bud tissues of nine peach genotypes from Embrapa's peach breeding program with different chilling requirements were used, and five candidate reference genes based on the RT-qPCR that were useful for studying the relative quantitative gene expression and stability were evaluated using geNorm, NormFinder, and bestKeeper software packages. The results indicated that among the genes tested, the most stable genes to be used as reference genes are Act and UBQ10. This study is the first survey of the stability of reference genes in peaches under chilling stress and provides guidelines for more accurate RT-qPCR results.
[Mh] Termos MeSH primário: Aclimatação/genética
Resposta ao Choque Frio/genética
Genótipo
Técnicas de Genotipagem/normas
Reação em Cadeia da Polimerase/normas
Prunus persica/genética
[Mh] Termos MeSH secundário: Flores/genética
Genes Essenciais
Genes de Plantas
Marcadores Genéticos
Técnicas de Genotipagem/métodos
Melhoramento Vegetal/métodos
Reação em Cadeia da Polimerase/métodos
Prunus persica/fisiologia
Padrões de Referência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16029666



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