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[PMID]:27746040
[Au] Autor:Yaba A; Sozen B; Suzen B; Demir N
[Ad] Endereço:Department of histology and embryology, Yeditepe university, faculty of medicine, 34755 Istanbul, Turkey. Electronic address: aylinyaba@hotmail.com.
[Ti] Título:Expression of aquaporin-7 and aquaporin-9 in tanycyte cells and choroid plexus during mouse estrus cycle.
[So] Source:Morphologie;101(332):39-46, 2017 Mar.
[Is] ISSN:1286-0115
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Tanycytes are special ependymal cells located in the ventrolateral wall and floor of the third ventricle having processes extending nuclei that regulate reproductive functions and around of vessels in median eminance. The aquaporins (AQPs) are a family of transmembrane proteins that transport water and glycerol. AQP-7 and -9 are permeable to other small molecules as glycerol and therefore called aquaglyceroporins. In this study, we aimed to show localization of AQP-7 and -9 in epithelial cells of choroid plexus and tanycytes during female mouse estrus cycle. AQP-7 and -9 proteins were detected in α2 and ß1 tanycytes in prÅ“strus stage. Interestingly, there is no staining in estrus stage in any type of tanycytes. We observed weak immunoreactivity in α1, α2 and ß1 tanycyte cells in metestrus stage for AQP-7 and α1 for AQP-9 protein. AQP-7 and -9 showed intense immunoreactivity in α2, ß1 and ß2 tanycyte cells during diestrus stage. Consequently, AQP-7 and -9 showed differential staining pattern in different stages of mouse estrus cycle. In the light of our findings and other recent publications, we suggest that AQP-7 and -9-mediated glycerol transport in tanycyte cells might be under hormonal control to use glycerol as a potential energy substrate during mouse estrus cycle.
[Mh] Termos MeSH primário: Aquaporinas/metabolismo
Plexo Corióideo/metabolismo
Células Ependimogliais/metabolismo
Ciclo Estral/metabolismo
[Mh] Termos MeSH secundário: Animais
Transporte Biológico
Plexo Corióideo/citologia
Células Epiteliais/metabolismo
Estro/metabolismo
Feminino
Glicerol/metabolismo
Metestro/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
Proestro/metabolismo
Terceiro Ventrículo/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aqp7 protein, mouse); 0 (Aqp9 protein, mouse); 0 (Aquaporins); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE


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[PMID]:26709901
[Au] Autor:Martin-DeLeon PA
[Ad] Endereço:219 Mckinly Lab, Department of Biological Sciences, University of Delaware, Newark, DE 19701, pdeleon@udel.edu.
[Ti] Título:Uterosomes: Exosomal cargo during the estrus cycle and interaction with sperm.
[So] Source:Front Biosci (Schol Ed);8:115-22, 2016 Jan 01.
[Is] ISSN:1945-0524
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The term "uterosomes" was first used to classify extracellular membrane vesicles released into the uterine luminal fluid. These extracellular vesicles (EVs), varying in sizes, fit the classification of exosomes and microvesicles on the basis of size, the presence of the CD9 biochemical marker, and lateral orientation of the membrane. Uterosomes appear to be formed by the apocrine pathway, similar to other reproductive EVs. In the murine system, the protein cargo carried by uterosomes includes glycosyl phosphatidylinositol (GPI)-linked and transmembrane proteins and these are hormonally regulated, appearing at high levels during proestrus/estrus and only marginally present at diestrus /metestrus. Uterosomes have been shown to deliver proteins in their cargo to sperm, with a functional impact, and are thought to participate in promoting sperm capacitation. Further studies are warranted, particularly those aimed at identifying the contents of their cargo during the estrus and menstrual cycle and the role they play n sperm maturation.
[Mh] Termos MeSH primário: Ciclo Estral
Exossomos/fisiologia
Útero/fisiologia
[Mh] Termos MeSH secundário: Animais
Estro
Feminino
Fertilização
Seres Humanos
Masculino
Metestro
Biogênese de Organelas
Proteínas/metabolismo
Reprodução
Maturação do Esperma
Espermatozoides
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; REVIEW
[Nm] Nome de substância:
0 (Proteins)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151229
[Lr] Data última revisão:
151229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151229
[St] Status:MEDLINE


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[PMID]:26653570
[Au] Autor:Woitowich NC; Philibert KD; Leitermann RJ; Wungjiranirun M; Urban JH; Glucksman MJ
[Ad] Endereço:Departments of Physiology and Biophysics (N.C.W., R.J.L., J.H.U.) and Biochemistry and Molecular Biology (N.C.W., K.D.P., M.W., M.J.G.), and Midwest Proteome Center (K.D.P., M.J.G.). Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois 60064.
[Ti] Título:EP24.15 as a Potential Regulator of Kisspeptin Within the Neuroendocrine Hypothalamus.
[So] Source:Endocrinology;157(2):820-30, 2016 Feb.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The neuropeptide kisspeptin (Kiss1) is integral to the advent of puberty and the generation of cyclical LH surges. Although many complex actions of Kiss1 are known, the mechanisms governing the processing/regulation of this peptide have not been unveiled. The metallo enzyme, endopeptidase 24.15 (thimet oligopeptidase), has been demonstrated to play a key role in the processing and thus the duration of action of the reproductive neuropeptide, GnRH, which signals downstream of Kiss1. Initial in silico modeling implied that Kiss1 could also be a putative substrate for EP24.15. Coincubation of Kiss1 and EP24.15 demonstrated multiple cleavages of the peptide predominantly between Arg29-Gly30 and Ser47-Phe48 (corresponding to Ser5-Phe6 in Kiss-10; Kiss-10 as a substrate had an additional cleavage between Phe6-Gly7) as determined by mass spectrometry. Vmax for the reaction was 2.37±0.09 pmol/min · ng with a Km of 19.68 ± 2.53µM, which is comparable with other known substrates of EP24.15. EP24.15 immunoreactivity, as previously demonstrated, is distributed in cell bodies, nuclei, and processes throughout the hypothalamus. Kiss1 immunoreactivity is localized primarily to cell bodies and fibers within the mediobasal and anteroventral-periventricular hypothalamus. Double-label immunohistochemistry indicated coexpression of EP24.15 and Kiss1, implicating that the regulation of Kiss1 by EP24.15 could occur in vivo. Further studies will be directed at determining the precise temporal sequence of EP24.15 effects on Kiss1 as it relates to the control of reproductive hormone secretion and treatment of fertility issues.
[Mh] Termos MeSH primário: Hormônio Liberador de Gonadotropina/metabolismo
Hipotálamo/enzimologia
Kisspeptinas/metabolismo
Metaloendopeptidases/metabolismo
[Mh] Termos MeSH secundário: Animais
Simulação por Computador
Escherichia coli
Feminino
Hipotálamo/metabolismo
Imuno-Histoquímica
Masculino
Espectrometria de Massas
Metestro/metabolismo
Proestro/metabolismo
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Kiss1 protein, rat); 0 (Kisspeptins); 33515-09-2 (Gonadotropin-Releasing Hormone); EC 3.4.24.- (Metalloendopeptidases); EC 3.4.24.15 (thimet oligopeptidase)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170201
[Lr] Data última revisão:
170201
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:151215
[St] Status:MEDLINE
[do] DOI:10.1210/en.2015-1580


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[PMID]:26632610
[Au] Autor:Hasegawa A; Mochida K; Inoue H; Noda Y; Endo T; Watanabe G; Ogura A
[Ad] Endereço:RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
[Ti] Título:High-Yield Superovulation in Adult Mice by Anti-Inhibin Serum Treatment Combined with Estrous Cycle Synchronization.
[So] Source:Biol Reprod;94(1):21, 2016 Jan.
[Is] ISSN:1529-7268
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Producing many mature oocytes is of great importance for assisted reproductive technologies. In mice, superovulation by consecutive injections of equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) has been the gold standard for oocyte collection. However, the yield of mature oocytes by this regimen can fluctuate according to the stage of the estrous cycle, strain, and age. Therefore, our objective was to develop a high-yield superovulation protocol to collect higher numbers of oocytes from adult female mice of different strains and ages. First, we aimed to synchronize the estrous cycle using C57BL/6 (B6) female mice. Most (93%) were synchronized to metestrus after two daily injections of progesterone. Second, we found that with the injection of anti-inhibin serum (AIS) instead of eCG, the mean number of ovulated oocytes almost doubled (21 vs. 41 per mouse). Third, by combining estrous cycle synchronization with two AIS injections, we obtained 62 oocytes per mouse, about three times that with the eCG-hCG protocol. Importantly, this approach increased the proportion of mice that ovulated >25 oocytes from about 40% (eCG-hCG) to 90%. The same protocol was also effective in other inbred (BALB/cA), outbred (ICR), and hybrid (B6D2F1) strains. In addition, B6 female mice aged over 1 yr ovulated 1.8-fold more oocytes by this protocol. Thus, estrous cycle synchronization followed by AIS-hCG yielded a broadly applicable, highly efficient superovulation. This protocol should promote the effective use of invaluable female mouse strains and decrease the numbers of animals euthanized.
[Mh] Termos MeSH primário: Anticorpos Bloqueadores/farmacologia
Sincronização do Estro/efeitos dos fármacos
Inibinas/antagonistas & inibidores
Inibinas/imunologia
Superovulação/efeitos dos fármacos
[Mh] Termos MeSH secundário: Envelhecimento
Animais
Gonadotropina Coriônica/farmacologia
Feminino
Fertilização In Vitro/métodos
Hormônio Foliculoestimulante/sangue
Metestro/fisiologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos ICR
Oócitos
Gravidez
Progesterona/farmacologia
Zona Pelúcida/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Blocking); 0 (Chorionic Gonadotropin); 4G7DS2Q64Y (Progesterone); 57285-09-3 (Inhibins); 9002-68-0 (Follicle Stimulating Hormone)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151204
[St] Status:MEDLINE
[do] DOI:10.1095/biolreprod.115.134023


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[PMID]:25925152
[Au] Autor:Vastagh C; Rodolosse A; Solymosi N; Farkas I; Auer H; Sárvári M; Liposits Z
[Ad] Endereço:Laboratory of Endocrine Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
[Ti] Título:Differential Gene Expression in Gonadotropin-Releasing Hormone Neurons of Male and Metestrous Female Mice.
[So] Source:Neuroendocrinology;102(1-2):44-59, 2015.
[Is] ISSN:1423-0194
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Gonadotropin-releasing hormone (GnRH) neurons play a pivotal role in the regulation of the hypothalamic-pituitary gonadal axis in a sex-specific manner. We hypothesized that the differences seen in reproductive functions of males and females are associated with a sexually dimorphic gene expression profile of GnRH neurons. METHODS AND RESULTS: We compared the transcriptome of GnRH neurons obtained from intact metestrous female and male GnRH-green fluorescent protein transgenic mice. About 1,500 individual GnRH neurons from each sex were sampled with laser capture microdissection followed by whole-transcriptome amplification for gene expression profiling. Under stringent selection criteria (fold change >1.6, adjusted p value 0.01), Affymetrix Mouse Genome 430 PM array analysis identified 543 differentially expressed genes. Sexual dimorphism was most apparent in gene clusters associated with synaptic communication, signal transduction, cell adhesion, vesicular transport and cell metabolism. To validate microarray results, 57 genes were selected, and 91% of their differential expression was confirmed by real-time PCR. Similarly, 88% of microarray results were confirmed with PCR from independent samples obtained by patch pipette harvesting and pooling of 30 GnRH neurons from each sex. We found significant differences in the expression of genes involved in vesicle priming and docking (Syt1, Cplx1), GABAergic (Gabra3, Gabrb3, Gabrg2) and glutamatergic (Gria1, Grin1, Slc17a6) neurotransmission, peptide signaling (Sstr3, Npr2, Cxcr4) and the regulation of intracellular ion homeostasis (Cacna1, Cacnb1, Cacng5, Kcnq2, Kcnc1). CONCLUSION: The striking sexual dimorphism of the GnRH neuron transcriptome we report here contributes to a better understanding of the differences in cellular mechanisms of GnRH neurons in the two sexes.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Hormônio Liberador de Gonadotropina/metabolismo
Neurônios/metabolismo
Caracteres Sexuais
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Feminino
Proteínas de Fluorescência Verde
Masculino
Metestro/genética
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
147336-22-9 (Green Fluorescent Proteins); 33515-09-2 (Gonadotropin-Releasing Hormone)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150919
[Lr] Data última revisão:
150919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150501
[St] Status:MEDLINE
[do] DOI:10.1159/000430818


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[PMID]:25787929
[Au] Autor:Okame R; Nakahara K; Murakami N
[Ad] Endereço:Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
[Ti] Título:Plasma amino acid profiles at various reproductive stages in female rats.
[So] Source:J Vet Med Sci;77(7):815-21, 2015 Jul.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:We measured the plasma levels of amino acids at various reproductive stages in female rats, including the estrous cycle, pregnancy and lactation, and compared the resulting amino acid profiles using two- or three-dimensional figures. These figures revealed that the amino acid profiles of pregnant and lactating dams differed considerably from those during the estrous cycle or in male rats. The plasma levels of individual amino acids were almost the same between proestrus, estrus, metestrus and diestrus, and their profiles did not differ significantly. However, the amino acid profiles changed during pregnancy and lactation in dams. The plasma Ser level decreased significantly in mid and late pregnancy, whereas Tyr, Gly and His decreased significantly in the late and end stages of pregnancy, and Trp and Lys significantly decreased and increased at the end of pregnancy, respectively. Much larger changes in amino acid profiles were observed during lactation, when the levels of many amino acids increased significantly, and none showed a significant decrease. Plasma Pro, Ser and Gly levels increased continuously from day 1 until day 15 of lactation, whereas Asn and Met increased significantly from days 1 and 5 respectively until the end of lactation. These results suggest that the profiles of plasma amino acids show characteristic changes according to reproductive stage and that it may be necessary to consider such differences when performing amino acid-based diagnosis.
[Mh] Termos MeSH primário: Aminoácidos/sangue
Ciclo Estral/sangue
Lactação/sangue
Prenhez/sangue
Ratos/sangue
[Mh] Termos MeSH secundário: Aminoácidos/fisiologia
Animais
Ciclo Estral/fisiologia
Estro/sangue
Estro/fisiologia
Feminino
Lactação/fisiologia
Masculino
Metestro/sangue
Metestro/fisiologia
Gravidez
Prenhez/fisiologia
Proestro/sangue
Proestro/fisiologia
Ratos/fisiologia
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150320
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.15-0095


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[PMID]:25296014
[Au] Autor:Kennedy LH; Hwang H; Wolfe AM; Hauptman J; Nemzek-Hamlin JA
[Ad] Endereço:Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, Michigan, USA. Lucy.kennedy@case.edu.
[Ti] Título:Effects of buprenorphine and estrous cycle in a murine model of cecal ligation and puncture.
[So] Source:Comp Med;64(4):270-82, 2014 Aug.
[Is] ISSN:1532-0820
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effect of opioids on the immunopathology of sepsis models in mice has been controversial. In previous work, we showed that mortality and various inflammatory parameters did not differ between female mice given saline or buprenorphine after cecal ligation and puncture. To investigate further, we hypothesized that buprenorphine would not affect outcomes of sepsis at any stage of estrous. Female mice were allocated into 4 groups (n = 20 per group) according to stage of estrous. Mice then underwent cecal ligation and puncture and received either buprenorphine or saline. In 3-wk survival studies, overall survival did not differ between buprenorphine- and saline-treated mice. When mice were stratified according to stage of estrous, survival did not vary among saline-treated groups but was lower in buprenorphine-treated mice in metestrus compared with proestrus. To investigate inflammation as a potential mechanism for survival, we measured cell counts and cytokine levels in the peripheral blood and peritoneal lavage fluid at 12 and 24 h after cecal ligation and puncture. At 24 h, buprenorphine-treated mice in proestrus had more circulating neutrophils and monocytes than did saline-treated mice in proestrus and more circulating WBC than did mice in any other stage with or without buprenorphine. Our current results suggest that the effects of buprenorphine on a 50% survival model of sepsis in BALB/c female mice are minimal overall but that the stage of estrous has various effects in this model. Investigators should consider the effects of buprenorphine and estrous cycle when using female mice in sepsis research.
[Mh] Termos MeSH primário: Analgésicos Opioides/farmacologia
Buprenorfina/farmacologia
Ceco/cirurgia
Ciclo Estral
Sepse/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Líquido Ascítico/imunologia
Líquido Ascítico/microbiologia
Comportamento Animal/efeitos dos fármacos
Ceco/microbiologia
Citocinas/sangue
Modelos Animais de Doenças
Estro
Feminino
Mediadores da Inflamação/sangue
Ligadura
Metestro
Camundongos
Camundongos Endogâmicos BALB C
Peritônio/efeitos dos fármacos
Peritônio/imunologia
Peritônio/microbiologia
Proestro
Punções
Sepse/sangue
Sepse/imunologia
Sepse/microbiologia
Sepse/fisiopatologia
Fatores Sexuais
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Analgesics, Opioid); 0 (Cytokines); 0 (Inflammation Mediators); 40D3SCR4GZ (Buprenorphine)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:151029
[Lr] Data última revisão:
151029
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141009
[St] Status:MEDLINE


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[PMID]:24800255
[Au] Autor:Rodríguez-Landa JF; Vicente-Serna J; Rodríguez-Blanco LA; Rovirosa-Hernández Mde J; García-Orduña F; Carro-Juárez M
[Ad] Endereço:Instituto de Neuroetología, Universidad Veracruzana, Avenida Dr. Luis Castelazo s/n, Colonia Industrial Ánimas, 91001 Xalapa, VER, Mexico ; Facultad de Química Farmacéutica Biológica, Universidad Veracruzana, 91190 Xalapa, VER, Mexico.
[Ti] Título:Montanoa frutescens and Montanoa grandiflora extracts reduce anxiety-like behavior during the metestrus-diestrus phase of the ovarian cycle in Wistar rats.
[So] Source:Biomed Res Int;2014:938060, 2014.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In previous studies, the anxiolytic-like effects of Montanoa tomentosa and Montanoa frutescens were reported in male rats, but the potential anxiolytic-like effects of Montanoa plants during the different phases of the ovarian cycle in rats remain to be explored. The anxiolytic-like effects of the aqueous crude extracts of M. frutescens (25 and 50 mg/kg) and M. grandiflora (25 and 50 mg/kg) in the elevated plus maze were investigated in Wistar rats during the estrous cycle and compared with 2 mg/kg diazepam as a reference anxiolytic drug. To investigate any motor effect (i.e., hyperactivity, no changes, or hypoactivity) associated with the treatments, the rats were evaluated in the open field test. The M. frutescens (25 and 50 mg/kg) and M. grandiflora (50 mg/kg) extracts exerted anxiolytic-like effects during the metestrus-diestrus phase, similar to diazepam, without disrupting spontaneous motor activity. No significant effects of the extracts were detected in either behavioral test during the proestrus-estrus phase, whereas diazepam produced motor hypoactivity in the open field test. These results indicate that the M. frutescens and M. grandiflora extracts possess anxiolytic-like effects that depend on the ovarian cycle phase, supporting the Mexican ancient medicinal use of these plants to ameliorate anxiety disorders.
[Mh] Termos MeSH primário: Ansiolíticos/farmacologia
Ansiedade/tratamento farmacológico
Diestro/fisiologia
Metestro/fisiologia
Montanoa/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Ansiolíticos/química
Ansiolíticos/uso terapêutico
Comportamento Animal/efeitos dos fármacos
Feminino
Aprendizagem em Labirinto/efeitos dos fármacos
Extratos Vegetais/química
Extratos Vegetais/uso terapêutico
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Anxiety Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:150806
[Lr] Data última revisão:
150806
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140507
[St] Status:MEDLINE
[do] DOI:10.1155/2014/938060


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[PMID]:24124622
[Au] Autor:Farkas I; Vastagh C; Sárvári M; Liposits Z
[Ad] Endereço:Laboratory of Endocrine Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
[Ti] Título:Ghrelin decreases firing activity of gonadotropin-releasing hormone (GnRH) neurons in an estrous cycle and endocannabinoid signaling dependent manner.
[So] Source:PLoS One;8(10):e78178, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The orexigenic peptide, ghrelin is known to influence function of GnRH neurons, however, the direct effects of the hormone upon these neurons have not been explored, yet. The present study was undertaken to reveal expression of growth hormone secretagogue receptor (GHS-R) in GnRH neurons and elucidate the mechanisms of ghrelin actions upon them. Ca(2+)-imaging revealed a ghrelin-triggered increase of the Ca(2+)-content in GT1-7 neurons kept in a steroid-free medium, which was abolished by GHS-R-antagonist JMV2959 (10 µM) suggesting direct action of ghrelin. Estradiol (1nM) eliminated the ghrelin-evoked rise of Ca(2+)-content, indicating the estradiol dependency of the process. Expression of GHS-R mRNA was then confirmed in GnRH-GFP neurons of transgenic mice by single cell RT-PCR. Firing rate and burst frequency of GnRH-GFP neurons were lower in metestrous than proestrous mice. Ghrelin (40 nM-4 µM) administration resulted in a decreased firing rate and burst frequency of GnRH neurons in metestrous, but not in proestrous mice. Ghrelin also decreased the firing rate of GnRH neurons in males. The ghrelin-evoked alterations of the firing parameters were prevented by JMV2959, supporting the receptor-specific actions of ghrelin on GnRH neurons. In metestrous mice, ghrelin decreased the frequency of GABAergic mPSCs in GnRH neurons. Effects of ghrelin were abolished by the cannabinoid receptor type-1 (CB1) antagonist AM251 (1µM) and the intracellularly applied DAG-lipase inhibitor THL (10 µM), indicating the involvement of retrograde endocannabinoid signaling. These findings demonstrate that ghrelin exerts direct regulatory effects on GnRH neurons via GHS-R, and modulates the firing of GnRH neurons in an ovarian-cycle and endocannabinoid dependent manner.
[Mh] Termos MeSH primário: Potenciais de Ação/efeitos dos fármacos
Endocanabinoides/metabolismo
Ciclo Estral/efeitos dos fármacos
Grelina/farmacologia
Hormônio Liberador de Gonadotropina/biossíntese
Neurônios/efeitos dos fármacos
Neurônios/fisiologia
Transdução de Sinais/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Encéfalo/metabolismo
Cálcio/metabolismo
Feminino
Neurônios GABAérgicos/efeitos dos fármacos
Neurônios GABAérgicos/fisiologia
Expressão Gênica
Masculino
Metestro/efeitos dos fármacos
Camundongos
Proestro/efeitos dos fármacos
RNA Mensageiro/genética
Receptores de Grelina/genética
Receptores de Grelina/metabolismo
Potenciais Sinápticos/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Endocannabinoids); 0 (Ghrelin); 0 (RNA, Messenger); 0 (Receptors, Ghrelin); 33515-09-2 (Gonadotropin-Releasing Hormone); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1405
[Cu] Atualização por classe:150422
[Lr] Data última revisão:
150422
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131015
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0078178


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[PMID]:23633624
[Au] Autor:Bhutada S; Katkam RR; Nandedkar T; Metkari SM; Chaudhari UK; Varghese S; Kholkute SD; Sachdeva G
[Ad] Endereço:Primate Biology Division, National Institute for Research in Reproductive Health, Indian Council of Medical Research, JM Street, Parel, Mumbai 400012, India.
[Ti] Título:Uterine secretome and its modulation in rat (Rattus norvegicus).
[So] Source:Reproduction;146(1):13-26, 2013 Jul.
[Is] ISSN:1741-7899
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study identifies uterine fluid (UF) proteins that display differential abundance during the embryo-permissive phase in nonconception and conception cycles in rats. UF samples were collected from nonpregnant rats in the proestrous (n=17) and metestrous (n=18) phases and also from pregnant (n=17) and pseudopregnant (n=17) rats on day 4 post coitus. UF protein profile in the metestrous phase was compared with that in the proestrous phase. Similarly, UF protein profile of the pregnant rats was compared with that of the pseudopregnant rats. Two-dimensional PAGE, followed by densitometric analysis of the paired protein spots, revealed differential abundance of 44 proteins in the metestrous phase, compared with that in the proestrous phase. Of these, 29 proteins were identified by matrix-assisted laser desorption/ionization time-of-flight or liquid chromatography-tandem mass spectrometry. Functional groups such as proteases, protease inhibitors, and oxidoreductases were enriched in differentially abundant proteins. Total protease activity in UF was found to be significantly (P<0.05; t-test) higher in the proestrous phase, compared with that in the metestrous phase. Furthermore, 41 UF proteins were found to be differentially abundant in pregnant rats, compared with pseudopregnant rats. Of these, 11 proteins could be identified. Immunoblotting analysis confirmed significantly higher (P<0.05; t-test) abundance of ß-actin, Rho-specific guanine nucleotide dissociation inhibitor alpha (Rho-GDIα), and peroxiredoxin-2 and -6 in the metestrous phase, compared with that in the proestrous phase. Compared with pseudopregnant rats, pregnant rats had significantly higher (P<0.05; t-test) levels of UF ß-actin and Rho-GDIα. Furthermore, these proteins could be detected in the culture supernatants of endometrial epithelial cell lines, thereby providing an evidence of their secretion from endometrial epithelial cells. Data obtained from the study expand our knowledge on the uterine milieu that favours embryo implantation.
[Mh] Termos MeSH primário: Prenhez/fisiologia
Útero/secreção
[Mh] Termos MeSH secundário: Actinas/metabolismo
Animais
Linhagem Celular
Eletroforese em Gel Bidimensional
Implantação do Embrião
Endométrio/metabolismo
Feminino
Seres Humanos
Metestro/fisiologia
Peptídeo Hidrolases/metabolismo
Peroxirredoxina VI/metabolismo
Gravidez
Pseudogravidez/metabolismo
Ratos
Inibidor alfa de Dissociação do Nucleotídeo Guanina rho/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins); 0 (rho Guanine Nucleotide Dissociation Inhibitor alpha); EC 1.11.1.15 (Peroxiredoxin VI); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1402
[Cu] Atualização por classe:130617
[Lr] Data última revisão:
130617
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130502
[St] Status:MEDLINE
[do] DOI:10.1530/REP-12-0461



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