Base de dados : MEDLINE
Pesquisa : G08.686.195.875 [Categoria DeCS]
Referências encontradas : 1475 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 148 ir para página                         

  1 / 1475 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28977586
[Au] Autor:Payrits M; Sághy É; Cseko K; Pohóczky K; Bölcskei K; Ernszt D; Barabás K; Szolcsányi J; Ábrahám IM; Helyes Z; Szoke É
[Ad] Endereço:Department of Pharmacology and Pharmacotherapy, University of Pécs Medical School, H-7624 Pécs, Hungary.
[Ti] Título:Estradiol Sensitizes the Transient Receptor Potential Vanilloid 1 Receptor in Pain Responses.
[So] Source:Endocrinology;158(10):3249-3258, 2017 Oct 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sex differences exist in chronic pain pathologies, and gonadal estradiol (E2) alters the pain sensation. The nocisensor transient receptor potential vanilloid 1 (TRPV1) receptor plays a critical role in triggering pain. Here we examined the impact of E2 on the function of TRPV1 receptor in mice sensory neurons in vitro and in vivo. Both mechano- and thermonociceptive thresholds of the plantar surface of the paw of female mice were significantly lower in proestrus compared with the estrus phase. These thresholds were higher in ovariectomized (OVX) mice and significantly lower in sham-operated mice in proestrus compared with the sham-operated mice in estrus phase. This difference was absent in TRPV1 receptor-deficient mice. Furthermore, E2 potentiated the TRPV1 receptor activation-induced mechanical hyperalgesia in OVX mice. Long pretreatment (14 hours) with E2 induced a significant increase in TRPV1 receptor messenger RNA expression and abolished the capsaicin-induced TRPV1 receptor desensitization in primary sensory neurons. The short E2 incubation (10 minutes) also prevented the desensitization, which reverted after coadministration of E2 and the tropomyosin-related kinase A (TrkA) receptor inhibitor. Our study provides in vivo and in vitro evidence for E2-induced TRPV1 receptor upregulation and sensitization mediated by TrkAR via E2-induced genomic and nongenomic mechanisms. The sensitization and upregulation of TRPV1 receptor by E2 in sensory neurons may explain the greater pain sensitivity in female mice.
[Mh] Termos MeSH primário: Estradiol/farmacologia
Dor/fisiopatologia
Canais de Cátion TRPV/efeitos dos fármacos
Canais de Cátion TRPV/fisiologia
[Mh] Termos MeSH secundário: Animais
Capsaicina/farmacologia
Células Cultivadas
Tolerância a Medicamentos
Estro/fisiologia
Feminino
Expressão Gênica/efeitos dos fármacos
Temperatura Alta
Masculino
Mecanorreceptores/efeitos dos fármacos
Mecanorreceptores/fisiologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Nociceptores/efeitos dos fármacos
Nociceptores/fisiologia
Ovariectomia
Proestro/fisiologia
RNA Mensageiro/análise
Células Receptoras Sensoriais/química
Células Receptoras Sensoriais/fisiologia
Caracteres Sexuais
Canais de Cátion TRPV/genética
Regulação para Cima/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (TRPV Cation Channels); 0 (TRPV1 receptor); 4TI98Z838E (Estradiol); S07O44R1ZM (Capsaicin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE
[do] DOI:10.1210/en.2017-00101


  2 / 1475 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28257870
[Au] Autor:De Los Reyes M; Palomino J; Parraguez VH; Ramirez F
[Ad] Endereço:Laboratory of Animal Reproduction, Faculty of Veterinary Sciences, University of Chile, Casilla 2 Correo 15, Santiago, Chile. Electronic address: mdlreyes@uchile.cl.
[Ti] Título:Analysis of LH receptor in canine ovarian follicles throughout the estrous cycle.
[So] Source:Theriogenology;93:71-77, 2017 Apr 15.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to determine the mRNA LHR and LHR protein expression pattern in the canine ovarian follicles at different stage of development throughout the estrous cycle. Dog ovaries were obtained from 1-6y bitches at proestrus/estrus, anestrus and diestrus stages following ovariohysterectomy. Follicular cells were mechanically recovered from follicles distributed into four types (preantral, small antral, medium antral and large antral). Total RNA extraction was performed and the evaluation of gene expression levels was achieved by relative quantification q-PCR analysis. Intrafollicular amounts of LHR were assessed by western blot method. All results were evaluated by ANOVA. The expression levels of mRNA LHR in follicular cells were observed in every stage of development, however this gene expression varied over the estrous cycle. LHR transcripts increased (P < 0.05) from preantral to antral stage. There were not differences in LHR gene expression among follicles at preantral stages; however, at antral stages the lowest (P < 0.05) LHR mRNA expression was found at anestrus and the highest (P < 0.05) at proestrus/estrus. The LHR protein was also detected in dog follicles in all reproductive phases with patterns varying with stage of follicular development over the reproductive cycle. The antibody against human LHR revealed two bands at ∼90 and ∼67 kDa, probably representing the matured protein and its precursor respectively. Both bands LHR appeared already at preantral follicles increasing (P < 0.05) with growth. A high proportion of LHR was presented as immature forms in all follicles stages during different phases of the estrous cycle. In conclusion, the gene and protein of LHR are differentially expressed in dog follicles over the estrous cycle, increasing with growth and the precursor protein is the most predominant LHR form present in canine follicles.
[Mh] Termos MeSH primário: Cães/metabolismo
Ciclo Estral/metabolismo
Folículo Ovariano/química
Receptores do LH/análise
Receptores do LH/genética
[Mh] Termos MeSH secundário: Anestro/metabolismo
Animais
Western Blotting/veterinária
Diestro/metabolismo
Estro/metabolismo
Feminino
Expressão Gênica
Histerectomia/veterinária
Ovariectomia/veterinária
Proestro/metabolismo
RNA Mensageiro/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (Receptors, LH)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE


  3 / 1475 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27911605
[Au] Autor:Silveira MA; Burger LL; DeFazio RA; Wagenmaker ER; Moenter SM
[Ad] Endereço:Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
[Ti] Título:GnRH Neuron Activity and Pituitary Response in Estradiol-Induced vs Proestrous Luteinizing Hormone Surges in Female Mice.
[So] Source:Endocrinology;158(2):356-366, 2017 02 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During the female reproductive cycle, estradiol exerts negative and positive feedback at both the central level to alter gonadotropin-releasing hormone (GnRH) release and at the pituitary to affect response to GnRH. Many studies of the neurobiologic mechanisms underlying estradiol feedback have been done on ovariectomized, estradiol-replaced (OVX+E) mice. In this model, GnRH neuron activity depends on estradiol and time of day, increasing in estradiol-treated mice in the late afternoon, coincident with a daily luteinizing hormone (LH) surge. Amplitude of this surge appears lower than in proestrous mice, perhaps because other ovarian factors are not replaced. We hypothesized GnRH neuron activity is greater during the proestrous-preovulatory surge than the estradiol-induced surge. GnRH neuron activity was monitored by extracellular recordings from fluorescently tagged GnRH neurons in brain slices in the late afternoon from diestrous, proestrous, and OVX+E mice. Mean GnRH neuron firing rate was low on diestrus; firing rate was similarly increased in proestrous and OVX+E mice. Bursts of action potentials have been associated with hormone release in neuroendocrine systems. Examination of the patterning of action potentials revealed a shift toward longer burst duration in proestrous mice, whereas intervals between spikes were shorter in OVX+E mice. LH response to an early afternoon injection of GnRH was greater in proestrous than diestrous or OVX+E mice. These observations suggest the lower LH surge amplitude observed in the OVX+E model is likely not attributable to altered mean GnRH neuron activity, but because of reduced pituitary sensitivity, subtle shifts in action potential pattern, and/or excitation-secretion coupling in GnRH neurons.
[Mh] Termos MeSH primário: Estradiol/fisiologia
Hormônio Liberador de Gonadotropina/fisiologia
Hormônio Luteinizante/sangue
Hipófise/fisiologia
Proestro/fisiologia
[Mh] Termos MeSH secundário: Potenciais de Ação
Animais
Feminino
Camundongos Endogâmicos C57BL
Neurônios/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
33515-09-2 (Gonadotropin-Releasing Hormone); 4TI98Z838E (Estradiol); 9002-67-9 (Luteinizing Hormone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161203
[St] Status:MEDLINE
[do] DOI:10.1210/en.2016-1771


  4 / 1475 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27746040
[Au] Autor:Yaba A; Sozen B; Suzen B; Demir N
[Ad] Endereço:Department of histology and embryology, Yeditepe university, faculty of medicine, 34755 Istanbul, Turkey. Electronic address: aylinyaba@hotmail.com.
[Ti] Título:Expression of aquaporin-7 and aquaporin-9 in tanycyte cells and choroid plexus during mouse estrus cycle.
[So] Source:Morphologie;101(332):39-46, 2017 Mar.
[Is] ISSN:1286-0115
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Tanycytes are special ependymal cells located in the ventrolateral wall and floor of the third ventricle having processes extending nuclei that regulate reproductive functions and around of vessels in median eminance. The aquaporins (AQPs) are a family of transmembrane proteins that transport water and glycerol. AQP-7 and -9 are permeable to other small molecules as glycerol and therefore called aquaglyceroporins. In this study, we aimed to show localization of AQP-7 and -9 in epithelial cells of choroid plexus and tanycytes during female mouse estrus cycle. AQP-7 and -9 proteins were detected in α2 and ß1 tanycytes in prÅ“strus stage. Interestingly, there is no staining in estrus stage in any type of tanycytes. We observed weak immunoreactivity in α1, α2 and ß1 tanycyte cells in metestrus stage for AQP-7 and α1 for AQP-9 protein. AQP-7 and -9 showed intense immunoreactivity in α2, ß1 and ß2 tanycyte cells during diestrus stage. Consequently, AQP-7 and -9 showed differential staining pattern in different stages of mouse estrus cycle. In the light of our findings and other recent publications, we suggest that AQP-7 and -9-mediated glycerol transport in tanycyte cells might be under hormonal control to use glycerol as a potential energy substrate during mouse estrus cycle.
[Mh] Termos MeSH primário: Aquaporinas/metabolismo
Plexo Corióideo/metabolismo
Células Ependimogliais/metabolismo
Ciclo Estral/metabolismo
[Mh] Termos MeSH secundário: Animais
Transporte Biológico
Plexo Corióideo/citologia
Células Epiteliais/metabolismo
Estro/metabolismo
Feminino
Glicerol/metabolismo
Metestro/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
Proestro/metabolismo
Terceiro Ventrículo/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aqp7 protein, mouse); 0 (Aqp9 protein, mouse); 0 (Aquaporins); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE


  5 / 1475 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:28011946
[Au] Autor:Salimi M; Zardooz H; Khodagholi F; Rostamkhani F; Shaerzadeh F
[Ad] Endereço:Department of Physiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
[Ti] Título:High-fat diet with stress impaired islets' insulin secretion by reducing plasma estradiol and pancreatic GLUT2 protein levels in rats' proestrus phase.
[So] Source:J Physiol Pharmacol;67(5):653-666, 2016 Oct.
[Is] ISSN:1899-1505
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:This study was conducted to determine whether two estrus phases (proestrus and diestrus) in female rats may influence the metabolic response to a high-fat diet and/or stress, focusing on pancreatic insulin secretion and content. Animals were divided into high-fat and normal diet groups, then each group was subdivided into stress and non-stress groups, and finally, each one of these was divided into proestrus and diestrus subgroups. At the end of high-fat diet treatment, foot-shock stress was applied to the animals. Then, blood samples were taken to measure plasma factors. Finally, the pancreas was removed for determination of glucose transporter 2 (GLUT2) protein levels and assessment of insulin content and secretion of the isolated islets. In the normal and high-fat diet groups, stress increased plasma corticosterone concentration in both phases. In both study phases, high-fat diet consumption decreased estradiol and increased leptin plasma levels. In the high-fat diet group in response to high glucose concentration, a reduction in insulin secretion was observed in the proestrus phase compared with the same phase in the normal diet group in the presence and absence of stress. Also, high-fat diet decreased the insulin content of islets in the proestrus phase compared with the normal diet. High-fat diet and/or stress caused a reduction in islet GLUT2 protein levels in both phases. In conclusion, it seems possible that high-fat diet alone or combined with foot-shock, predispose female rats to impaired insulin secretion, at least in part, by interfering with estradiol levels in the proestrus phase and decreasing pancreatic GLUT2 protein levels.
[Mh] Termos MeSH primário: Dieta Hiperlipídica
Estradiol/sangue
Transportador de Glucose Tipo 2/metabolismo
Insulina/secreção
Pâncreas/metabolismo
Proestro/metabolismo
[Mh] Termos MeSH secundário: Animais
Glicemia/análise
Corticosterona/sangue
Diestro/sangue
Diestro/metabolismo
Estimulação Elétrica
Feminino

Insulina/sangue
Ilhotas Pancreáticas/secreção
Proestro/sangue
Ratos Wistar
Estresse Fisiológico
Estresse Psicológico/sangue
Estresse Psicológico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Glucose Transporter Type 2); 0 (Insulin); 0 (Slc2a2 protein, rat); 4TI98Z838E (Estradiol); W980KJ009P (Corticosterone)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161225
[St] Status:MEDLINE


  6 / 1475 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27809846
[Au] Autor:Espinosa-Valdez A; Flores A; Arrieta-Cruz I; Cárdenas M; Chavira R; Domínguez R; Cruz ME
[Ad] Endereço:Biology of Reproduction Research Unit, Laboratory of Neuroendocrinology, Facultad de Estudios Superiores Zaragoza, UNAM, AP 9-020, CP 15000, Mexico City, Mexico.
[Ti] Título:The participation of the muscarinic receptors in the preoptic-anterior hypothalamic areas in the regulation of ovulation depends on the ovary.
[So] Source:Reprod Biol Endocrinol;14(1):75, 2016 Nov 04.
[Is] ISSN:1477-7827
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Muscarinic receptors (mAChRs) of the preoptic and anterior hypothalamus areas (POA-AHA) regulate ovulation in an asymmetric manner during the estrous cycle. The aims of the present study were to analyze the effects of a temporal blockade of mAChRs on either side of the POA-AHA performed in diestrus-2 rats on ovulation, the levels of estradiol, follicle stimulating hormone (FSH) and luteinizing hormone (LH) and the mechanisms involved in changes in ovulation. METHODS: Cyclic rats on diestrus-2 day were anesthetized and randomly assigned to the following groups: 1) microinjection of 1 µl of saline or atropine solution (62.5 ng) in the left or right POA-AHA; 2) removal (unilateral ovariectomty, ULO) of the left (L-ULO) or right (R-ULO) ovary, and 3) rats microinjected with atropine into the left or right POA-AHA plus L-ULO or R-ULO. The ovulation rate and the number of ova shed were measured during the predicted estrus, as well as the levels of estradiol, FSH and LH during the predicted proestrus and the effects of injecting synthetic LH-releasing hormone (LHRH) or estradiol benzoate (EB). RESULTS: Atropine in the left POA-AHA decreased both the ovulation rate and estradiol and LH levels on the afternoon of proestrus, also LHRH or EB injection restored ovulation. L- or R-ULO resulted in a lower ovulation rate and smaller number of ova shed, and only injection of LHRH restored ovulation. EB injection at diestrus-2 restored ovulation in animals with L-ULO only. The levels of estradiol, FSH and LH in rats with L-ULO were higher than in animals with unilateral laparotomy. In the group microinjected with atropine in the left POA-AHA, ovulation was similar to that in ULO rats. In contrast, atropine in the right POA-AHA of ULO rats blocked ovulation, an action that was restored by either LHRH or EB injection. CONCLUSIONS: These results indicated that the removal of a single ovary at noon on diestrus-2 day perturbed the neuronal pathways regulating LH secretion, which was mediated by the muscarinic system connecting the right POA-AHA and the ovaries.
[Mh] Termos MeSH primário: Núcleo Hipotalâmico Anterior/metabolismo
Diestro/metabolismo
Estradiol/metabolismo
Hormônio Foliculoestimulante/metabolismo
Hormônio Luteinizante/metabolismo
Ovulação/metabolismo
Área Pré-Óptica/metabolismo
Receptores Muscarínicos/metabolismo
[Mh] Termos MeSH secundário: Animais
Núcleo Hipotalâmico Anterior/efeitos dos fármacos
Atropina/farmacologia
Anticoncepcionais/farmacologia
Diestro/efeitos dos fármacos
Estradiol/análogos & derivados
Estradiol/farmacologia
Feminino
Hormônio Liberador de Gonadotropina/farmacologia
Hormônio Luteinizante/efeitos dos fármacos
Antagonistas Muscarínicos/farmacologia
Ovariectomia
Ovário/efeitos dos fármacos
Ovulação/efeitos dos fármacos
Área Pré-Óptica/efeitos dos fármacos
Proestro/efeitos dos fármacos
Proestro/metabolismo
Ratos
Receptores Muscarínicos/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Contraceptive Agents); 0 (Muscarinic Antagonists); 0 (Receptors, Muscarinic); 1S4CJB5ZGN (estradiol 3-benzoate); 33515-09-2 (Gonadotropin-Releasing Hormone); 4TI98Z838E (Estradiol); 7C0697DR9I (Atropine); 9002-67-9 (Luteinizing Hormone); 9002-68-0 (Follicle Stimulating Hormone)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE


  7 / 1475 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27306649
[Au] Autor:Vieyra E; Ramírez DA; Lagunas N; Cárdenas M; Chavira R; Damián-Matsumura P; Trujillo A; Domínguez R; Morales-Ledesma L
[Ad] Endereço:Biology of Reproduction Research Unit, Physiology of Reproduction Laboratory, Facultad de Estudios Superiores Zaragoza, UNAM, AP 9-020, CP 15000, Ciudad de México, México.
[Ti] Título:Unilaterally blocking the muscarinic receptors in the suprachiasmatic nucleus in proestrus rats prevents pre-ovulatory LH secretion and ovulation.
[So] Source:Reprod Biol Endocrinol;14(1):34, 2016 Jun 16.
[Is] ISSN:1477-7827
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The suprachiasmatic nucleus (SCN) and the cholinergic system of various regions of the hypothalamus participate in the regulation of gonadotropin-releasing hormone (GnRH) and gonadotropin secretion, which are necessary for the occurrence of ovulation. In the present study, our goal was to analyse the effects of unilaterally blocking the muscarinic receptors in the SCN on ovulation and steroid secretion. METHODS: Cyclic rats were randomly allotted to one of the experimental groups. Groups of 8-14 rats were anaesthetized and microinjected with 0.3 µl of saline or a solution of atropine (62.5 ng in 0.3 µl of saline) into the left or right SCN at 09.00 or 19.00 h during diestrus-1 or on the proestrus day. The rats were euthanized on the predicted day of oestrus, and evaluated ovulation and levels of progesterone and oestradiol. Other groups of 10 rats were microinjected with atropine into the left or right SCNs at 09.00 h on the proestrus day, were euthanized eight h later, and luteinizing hormone (LH) was measured. RESULTS: At 09.00 or 19.00 h during diestrus-1, atropine microinjections into the SCNs on either side did not modify ovulation. The atropine microinjections performed at 09.00 h of proestrus into either side of the SCN blocked ovulation (right SCN: 1/9 ovulated vs. 9/10 in the saline group; left SCN: 8/14 ovulated vs. 10/10 in the saline group). The LH levels at 17.00 h in the rats that were microinjected with atropine at 09.00 h of proestrus were lower than those of the controls. In the non-ovulating atropine-treated rats, the injection of synthetic LH-releasing hormone (LHRH) restored ovulation. Atropine treatment at 19.00 h of proestrus on either side of the SCN did not modify ovulation, while the progesterone and oestradiol levels were lower. CONCLUSION: Based on the present results, we suggest that the cholinergic neural information arriving on either side of the SCN is necessary for the pre-ovulatory secretion of LH to induce ovulation. Additionally, the regulation of progesterone and oestradiol secretion by the cholinergic innervation of the SCN varies with the time of day, the day of the cycle, and the affected SCN.
[Mh] Termos MeSH primário: Atropina/farmacologia
Hormônio Luteinizante/sangue
Antagonistas Muscarínicos/farmacologia
Ovulação/efeitos dos fármacos
Proestro/efeitos dos fármacos
Núcleo Supraquiasmático/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Feminino
Ovário/efeitos dos fármacos
Proestro/metabolismo
Ratos
Núcleo Supraquiasmático/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Muscarinic Antagonists); 7C0697DR9I (Atropine); 9002-67-9 (Luteinizing Hormone)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160617
[St] Status:MEDLINE
[do] DOI:10.1186/s12958-016-0168-7


  8 / 1475 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27068699
[Au] Autor:Yap CC; Wharfe MD; Mark PJ; Waddell BJ; Smith JT
[Ad] Endereço:School of AnatomyPhysiology and Human Biology, The University of Western Australia, Perth, Western Australia, Australia.
[Ti] Título:Diurnal regulation of hypothalamic kisspeptin is disrupted during mouse pregnancy.
[So] Source:J Endocrinol;229(3):307-18, 2016 Jun.
[Is] ISSN:1479-6805
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Kisspeptin, the neuropeptide product of the Kiss1 gene, is critical in driving the hypothalamic-pituitary-gonadal (HPG) axis. Kisspeptin neurons in the anteroventral periventricular nucleus (AVPV) and arcuate nucleus (Arc) of the hypothalamus mediate differential effects, with the Arc regulating negative feedback of sex steroids and the AVPV regulating positive feedback, vital for the preovulatory surge and gated under circadian control. We aimed to characterize hypothalamic Kiss1 and Kiss1r mRNA expression in nonpregnant and pregnant mice, and investigate potential circadian regulation. Anterior and posterior hypothalami were collected from C57BL/6J mice at diestrus, proestrus, and days 6, 10, 14, and 18 of pregnancy, at six time points across 24h, for real-time PCR analysis of gene expression. Analysis confirmed that Kiss1 mRNA expression in the AVPV increased at ZT13 during proestrus, with a luteinizing hormone surge observed thereafter. No diurnal regulation was seen at diestrus or at any stage of pregnancy. Anterior hypothalamic Avp mRNA expression exhibited no diurnal variation, but Avpr1a peaked at 12:00h during proestrus, possibly reflecting the circadian input from the suprachiasmatic nucleus to AVPV Kiss1 neurons. Rfrp (Npvf) expression in the posterior hypothalamus did not demonstrate diurnal variation at any stage. Clock genes Bmal1 and Rev-erbα were strongly diurnal, but there was little change between diestrus/proestrus and pregnancy. Our data indicate the absence of the circadian input to Kiss1 in pregnancy, despite high gestational estradiol levels and normal clock gene expression, and may suggest a disruption of a kisspeptin-specific diurnal rhythm that operates in the nonpregnant state.
[Mh] Termos MeSH primário: Ritmo Circadiano/fisiologia
Hipotálamo/fisiologia
Kisspeptinas/fisiologia
Prenhez/fisiologia
[Mh] Termos MeSH secundário: Animais
Arginina Vasopressina/genética
Ritmo Circadiano/genética
Feminino
Expressão Gênica
Hormônios/sangue
Kisspeptinas/genética
Camundongos
Camundongos Endogâmicos C57BL
Gravidez
Prenhez/genética
Proestro/genética
Proestro/fisiologia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Receptores de Vasopressinas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AVPR1a receptor, mouse); 0 (Hormones); 0 (Kiss1 protein, mouse); 0 (Kisspeptins); 0 (RNA, Messenger); 0 (Receptors, Vasopressin); 113-79-1 (Arginine Vasopressin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160413
[St] Status:MEDLINE
[do] DOI:10.1530/JOE-16-0086


  9 / 1475 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26909900
[Au] Autor:Wang SB; Liu YP; Zhang ZH; Wang ZK; Xu YX; Wang ZC
[Ad] Endereço:Laboratory of Sport Physiology and Biomedicine, School of Physical Education and Sport Sciences, Fujian Normal University, Fuzhou, China.
[Ti] Título:Temporal and spatial expression profiles of Frizzled 3 in the ovary during the estrous cycle.
[So] Source:Genet Mol Res;15(1), 2016 Jan 08.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Frizzled 3 is an important receptor in the Wnt/ß-catenin pathway, a conserved signaling pathway that regulates gene expression and controls diverse developmental processes. However, the role of this protein during follicular development in the adult ovary is not known. The present study was designed to investigate the expression and localization of Frizzled 3 mRNA and protein during the estrous cycle in the mouse ovary through in situ hybridization (ISH), real-time quantitative polymerase chain reaction, immunohistochemistry and western blot. ISH results showed that in proestrus, high expression of Frizzled 3 was found in the granulosa and stroma with weak levels in the corpus luteum. In estrus and diestrus, the stroma had high Frizzled 3 expression, but levels were low in granulosa cells and corpus luteum. In the metestrus, moderate expression of Frizzled 3 was found in the stroma but low to no expression was found in luteal cells and follicles. The mRNA and protein levels of Frizzled 3 were found to be the highest in proestrus and diestrus compared to estrus and metestrus (P < 0.05), confirming the ISH results. During estrus and diestrus, high Frizzled 3 expression was observed in the stroma and moderate levels in granulosa cells, and during estrus and proestrus, low expression was seen in the oocyte cell membrane. The western blot results further confirmed this change during the estrous cycle. Together, these results indicate that Frizzled 3 is involved in regulating follicular development and oocyte maturation during the estrous cycle.
[Mh] Termos MeSH primário: Corpo Lúteo/metabolismo
Receptores Frizzled/genética
Regulação da Expressão Gênica no Desenvolvimento
Oócitos/metabolismo
Folículo Ovariano/metabolismo
[Mh] Termos MeSH secundário: Animais
Corpo Lúteo/crescimento & desenvolvimento
Corpo Lúteo/ultraestrutura
Diestro/genética
Estro/genética
Feminino
Receptores Frizzled/metabolismo
Hibridização In Situ
Camundongos
Camundongos Endogâmicos ICR
Oócitos/crescimento & desenvolvimento
Oócitos/ultraestrutura
Folículo Ovariano/crescimento & desenvolvimento
Folículo Ovariano/ultraestrutura
Proestro/genética
Reação em Cadeia da Polimerase em Tempo Real
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Frizzled Receptors); 0 (Fzd3 protein, mouse)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE
[do] DOI:10.4238/gmr.15017152


  10 / 1475 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26778278
[Au] Autor:Delsouc MB; Della Vedova MC; Ramírez D; Anzulovich AC; Delgado SM; Casais M
[Ad] Endereço:Laboratorio de Biología de la Reproducción (LABIR), Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina; Instituto Multidisciplinario de Investigaciones Biológicas de San Luis (IMIBIO-SL), Consejo Nacional de Investigaciones Científicas y Técnicas (CONIC
[Ti] Título:Oxidative stress and altered steroidogenesis in the ovary by cholinergic stimulation of coeliac ganglion in the first proestrous in rats. Implication of nitric oxide.
[So] Source:Nitric Oxide;53:45-53, 2016 Feb 29.
[Is] ISSN:1089-8611
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An ex-vivo Coeliac Ganglion-Superior Ovarian Nerve-Ovary (CG-SON-O) system from virgin rats in the first proestrous was used to test whether cholinergic stimulation of CG affects oxidative status and steroidogenesis in the ovary. The CG and the O were placed in separate buffered-compartments, connected by the SON, and the CG was stimulated by acetylcholine (Ach). To test a possible role of nitric oxide (NO) in the ovarian response to cholinergic stimulation of CG, aminoguanidine (AG) - an inhibitor of inducible-NO synthase was added to the O compartment. After 180 min incubation, the oxidative status was assessed in O whereas nitrite and steroidogenesis were assessed at 30, 120 and 180 min. Ach in CG decreased the total antioxidant capacity, but increased NO production and protein carbonization in O. Ach stimulation of CG increased estradiol, but decreased progesterone release in O by reducing the mRNAs related to their synthesis and degradation. The addition of AG to the O compartment caused an opposite effect, which was more pronounced in the presence of Ach in the CG compartment than in its absence. These results show that the stimulation of the extrinsic-cholinergic innervation of the O increases the concentration of NO, causes oxidative stress and modulates steroidogenesis in the first rat proestrous.
[Mh] Termos MeSH primário: Colinérgicos/farmacologia
Gânglios Simpáticos/efeitos dos fármacos
Óxido Nítrico/metabolismo
Ovário/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Proestro
Progesterona/biossíntese
[Mh] Termos MeSH secundário: Animais
Feminino
Gânglios Simpáticos/fisiologia
Óxido Nítrico/biossíntese
Ovário/inervação
Ovário/metabolismo
Proestro/efeitos dos fármacos
Progesterona/secreção
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cholinergic Agents); 31C4KY9ESH (Nitric Oxide); 4G7DS2Q64Y (Progesterone)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160119
[St] Status:MEDLINE



página 1 de 148 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde