Base de dados : MEDLINE
Pesquisa : G08.686.290 [Categoria DeCS]
Referências encontradas : 801 [refinar]
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  1 / 801 MEDLINE  
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[PMID]:28455549
[Au] Autor:Cassel M; de Paiva Camargo M; Oliveira de Jesus LW; Borella MI
[Ad] Endereço:Fish Endocrinology Laboratory, Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, Av Prof Lineu Prestes n. 1524, lab 426, São Paulo, SP, CEP 05508-000, Brazil. monica_cassel@usp.br.
[Ti] Título:Involution processes of follicular atresia and post-ovulatory complex in a characid fish ovary: a study of apoptosis and autophagy pathways.
[So] Source:J Mol Histol;48(3):243-257, 2017 Jun.
[Is] ISSN:1567-2387
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Recent studies seem to indicate that apoptosis and autophagy can act cooperatively in fish ovaries in order to achieve more effective ovarian regression after spawning. Considering the importance of tissue remodeling in ovarian functioning, we sought to morphologically characterize the involution processes of follicular atresia and post-ovulatory complexes using Astyanax altiparanae as an experimental model, and to determine the location of proteins involved in apoptosis and autophagy throughout this process. Fifteen females were collected after reproductive management. Fragments of the left ovaries were removed, fixed, and prepared for light microscopy and immunofluorescence analyses. The main characteristics of the involution processes were found to be consistent with previous descriptions. However, there were certain morphological peculiarities that do not appear to have been described for any other species thus far. These peculiarities may be related to the focus of this study on a single species, which allows for a more detailed investigation into morphological changes than studies on multiple species. Autophagy was also found to precede apoptosis in both involution processes in A. altiparanae. This may be related to the energy recycling process required before the removal of degenerated follicular cells by apoptosis. Thus, these results support the idea that there is crosstalk between autophagy and apoptosis pathways in ovarian involution processes, as well as the idea that the cell death pathways of these processes are conserved between teleost species with external fertilization.
[Mh] Termos MeSH primário: Characidae/fisiologia
Atresia Folicular
Ovário/fisiologia
[Mh] Termos MeSH secundário: Animais
Apoptose
Autofagia
Feminino
Redes e Vias Metabólicas
Receptor Cross-Talk
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1007/s10735-017-9723-6


  2 / 801 MEDLINE  
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[PMID]:28646569
[Au] Autor:Mirzaei M; Razi M; Sadrkhanlou R
[Ad] Endereço:Department of Basic Science, division of Comparative Histology & Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
[Ti] Título:Nanosilver particles increase follicular atresia: Correlation with oxidative stress and aromatization.
[So] Source:Environ Toxicol;32(10):2244-2255, 2017 Oct.
[Is] ISSN:1522-7278
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Present study was performed in order to update the possible mechanism(s), involving in nanosilver particles (NSPs)-induced detrimental impacts in ovarian tissue. For this purpose, 24 mature female rats were divided into control and 0.5, 1, 5 mg/kg NSPs-received groups (intraperitoneally, for 35 days). Follicular growth and atresia, ovarian total antioxidant capacity (TAC), malondialdehyde (MDA), superoxide dismutase (SOD) contents, serum estrogen (E ) level and macrophages infiltration were investigated. Moreover, ovarian angiogenesis, cellular mRNA damage and cytochrome aromatase CYP19 expression were analyzed. The NSPs enhanced follicular atresia diminished E reduced TAC and SOD level, elevated MDA content and up-regulated macrophages infiltration. Cellular mRNA damage, impaired angiogenesis and diminished CYP19 expression were revealed in NSPs-received groups. Therefore NSPs by down-regulating aromatization, reduce E synthesis which then it leads to impaired angiogenesis. The impaired angiogenesis in turn down-regulates ovarian antioxidant status, which partially enhances follicular atresia by triggering lipid peroxidation and mRNA damage.
[Mh] Termos MeSH primário: Aromatase/metabolismo
Atresia Folicular/efeitos dos fármacos
Nanopartículas Metálicas/toxicidade
Ovário/efeitos dos fármacos
Estresse Oxidativo
Prata/toxicidade
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Estradiol/sangue
Feminino
Peroxidação de Lipídeos/efeitos dos fármacos
Malondialdeído/metabolismo
Neovascularização Fisiológica/efeitos dos fármacos
Ovário/irrigação sanguínea
Ovário/metabolismo
RNA Mensageiro/metabolismo
Ratos Wistar
Superóxido Dismutase/metabolismo
Testosterona/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (RNA, Messenger); 3M4G523W1G (Silver); 3XMK78S47O (Testosterone); 4TI98Z838E (Estradiol); 4Y8F71G49Q (Malondialdehyde); EC 1.14.14.1 (Aromatase); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170625
[St] Status:MEDLINE
[do] DOI:10.1002/tox.22440


  3 / 801 MEDLINE  
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[PMID]:28333135
[Au] Autor:Yeung CK; Wang G; Yao Y; Liang J; Tenny Chung CY; Chuai M; Lee KK; Yang X
[Ad] Endereço:Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University, Guangzhou 510632, China.
[Ti] Título:BRE modulates granulosa cell death to affect ovarian follicle development and atresia in the mouse.
[So] Source:Cell Death Dis;8(3):e2697, 2017 Mar 23.
[Is] ISSN:2041-4889
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The BRE (brain and reproductive expression) gene, highly expressed in nervous and reproductive system organs, plays an important role in modulating DNA damage repair under stress response and pathological conditions. Folliculogenesis, a process that ovarian follicle develops into maturation, is closely associated with the interaction between somatic granulosa cell and oocyte. However, the regulatory role of BRE in follicular development remains undetermined. In this context, we found that BRE is normally expressed in the oocytes and granulosa cells from the primordial follicle stage. There was a reduction in follicles number of BRE mutant (BRE ) mice. It was attributed to increase the follicular atresia in ovaries, as a result of retarded follicular development. We established that cell proliferation was inhibited, while apoptosis was markedly increased in the granulosa cells in the absence of BRE. In addition, expressions of γ-H2AX (marker for showing DNA double-strand breaks) and DNA damage-relevant genes are both upregulated in BRE mice. In sum, these results suggest that the absence of BRE, deficiency in DNA damage repair, causes increased apoptosis in granulosa cells, which in turn induces follicular atresia in BRE mice.
[Mh] Termos MeSH primário: Morte Celular/fisiologia
Atresia Folicular/metabolismo
Células da Granulosa/metabolismo
Células da Granulosa/patologia
Proteínas do Tecido Nervoso/metabolismo
Proteínas Nucleares/metabolismo
Folículo Ovariano/metabolismo
Ovário/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/fisiologia
Linhagem Celular Tumoral
Proliferação Celular/fisiologia
Dano ao DNA/fisiologia
Reparo do DNA/fisiologia
Feminino
Atresia Folicular/fisiologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Oócitos/metabolismo
Folículo Ovariano/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bre protein, mouse); 0 (Nerve Tissue Proteins); 0 (Nuclear Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1038/cddis.2017.91


  4 / 801 MEDLINE  
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[PMID]:28261870
[Au] Autor:Grier HJ; Neidig CL; Quagio-Grassiotto I
[Ad] Endereço:Florida Fish and Wildlife Research Institute, 100 8th Ave, S, St. Petersburg, Florida, 33701-5020.
[Ti] Título:Development and fate of the postovulatory follicle complex, postovulatory follicle, and observations on folliculogenesis and oocyte atresia in ovulated common snook, Centropomus undecimalis (Bloch, 1792).
[So] Source:J Morphol;278(4):547-562, 2017 Apr.
[Is] ISSN:1097-4687
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The common snook, Centropomus undecimalis, was induced to ovulate using a time-release, GnRH analogue. Ovulation occurred the afternoon or evening the day after hormone administration. The time of ovulation was established within half an hour. At ovulation, three fish per time-group were divided into 0, 6, 12, 18 hr and one thru five days post-ovulation to study changes in the postovulatory follicle complex (POC). Histology of the ovaries revealed changes in the POC, postovulatory follicle (POF) and oocyte atresia through five days post-ovulation. Within 24 hr, nuclei of the POF cells lost their initial spherical or oval configuration, and by four days the basement membrane within the POC had fragmented. There was a temporal separation between ovulation and post-ovulation folliculogenesis; that is, in that the formation of new follicles commenced within the germinal epithelium between 12-48 hrs after ovulation. Morphology of the POC was best revealed with the reticulin stain; it is composed of the POF and postovulatory theca (POT). These are separated by a basement membrane, reflecting the origin of a follicle from a germinal epithelium while the theca is derived from stroma. The POF is composed of the former follicle cells that surrounded and contacted the oocyte during its development; the follicle is composed of the oocyte and its surrounding follicle cells. The POC is composed of a prominent basement membrane separating the POT from the POF. The reticulin stain clearly defines compartmentation in the ovary and supports redefinition of the POF as the follicle cells that formerly surrounded the oocyte prior to ovulation. J. Morphol. 278:547-562, 2017. © 2017 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Atresia Folicular/fisiologia
Oócitos/citologia
Organogênese
Folículo Ovariano/citologia
Folículo Ovariano/crescimento & desenvolvimento
Ovulação/fisiologia
Perciformes/crescimento & desenvolvimento
Perciformes/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170307
[St] Status:MEDLINE
[do] DOI:10.1002/jmor.20652


  5 / 801 MEDLINE  
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[PMID]:28208755
[Au] Autor:Worku T; Rehman ZU; Talpur HS; Bhattarai D; Ullah F; Malobi N; Kebede T; Yang L
[Ad] Endereço:Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. wtesfaye68@yahoo.com.
[Ti] Título:MicroRNAs: New Insight in Modulating Follicular Atresia: A Review.
[So] Source:Int J Mol Sci;18(2), 2017 Feb 09.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Our understanding of the post-transcriptional mechanisms involved in follicular atresia is limited; however, an important development has been made in understanding the biological regulatory networks responsible for mediating follicular atresia. MicroRNAs have come to be seen as a key regulatory actor in determining cell fate in a wide range of tissues in normal and pathological processes. Profiling studies of miRNAs during follicular atresia and development have identified several putative miRNAs enriched in apoptosis signaling pathways. Subsequent in vitro and/or in vivo studies of granulosa cells have elucidated the functional role of some miRNAs along with their molecular pathways. In particular, the regulatory roles of some miRNAs have been consistently observed during studies of follicular cellular apoptosis. Continued work should gradually lead to better understanding of the role of miRNAs in this field. Ultimately, we expect this understanding will have substantial benefits for fertility management at both the in vivo or/and in vitro levels. The stable nature of miRNA holds remarkable promise in clinical use as a diagnostic tool and in reproductive medicine to solve the ever-increasing fertility problem. In this review, we summarize current knowledge of the involvement of miRNAs in follicular atresia, discuss the challenges for further work and pinpoint areas for future research.
[Mh] Termos MeSH primário: Atresia Folicular/genética
Regulação da Expressão Gênica
MicroRNAs/genética
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Autofagia/genética
Biomarcadores
Feminino
Atresia Folicular/metabolismo
Perfilação da Expressão Gênica
Células da Granulosa/metabolismo
Seres Humanos
Folículo Ovariano/metabolismo
Transdução de Sinais
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biomarkers); 0 (MicroRNAs)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE


  6 / 801 MEDLINE  
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[PMID]:28165147
[Au] Autor:Waltrick DS; Simpfendorfer CA; Awruch CA
[Ad] Endereço:Department of Fisheries, Western Australian Fisheries and Marine Research Laboratories, Government of Western Australia, PO Box 20, North Beach, Western Australia, 6920, Australia.
[Ti] Título:A review on the morphology of ovarian follicles in elasmobranchs: A case study in Rhizoprionodon taylori.
[So] Source:J Morphol;278(4):486-499, 2017 Apr.
[Is] ISSN:1097-4687
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The identification of the elasmobranch secondary ovarian follicles and their function can be challenging and the inconsistent use of terminology derived from other taxa is a matter of ongoing debate. In this study, the available information on the histology of the elasmobranch secondary ovarian follicles derived from atresia (preovulatory follicles) or ovulation (postovulatory follicles) is reviewed highlighting their morphology and steroidogenic capacity. Based on this literature review, the ovarian follicles of the Australian sharpnose shark Rhizoprionodon taylori were classified according to their preovulatory or postovulatory origin. Two types of secondary follicles originating from atresia of developing follicles (atretic previtellogenic follicles) and ripe follicles (atretic vitellogenic follicles), and one type of postovulatory follicle were identified throughout the reproductive year of this species. Morphological similarities of the elasmobranch secondary ovarian follicles and their variations in different species denote the difficulty to classify them. Given the multiple origins of ovarian follicles, their poorly understood functions and capacity to supply steroids, visual identification of these structures and the use of terminology derived from mammalian and other vertebrate studies (with the exception of the corpora lutea as a temporary endocrine gland) is not advisable. J. Morphol. 278:486-499, 2017. © 2017 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Folículo Ovariano/anatomia & histologia
Tubarões/anatomia & histologia
[Mh] Termos MeSH secundário: Animais
Feminino
Atresia Folicular/fisiologia
Oócitos/citologia
Ovulação
Vitelogênese/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170207
[St] Status:MEDLINE
[do] DOI:10.1002/jmor.20644


  7 / 801 MEDLINE  
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[PMID]:28086947
[Au] Autor:Uslu B; Dioguardi CC; Haynes M; Miao DQ; Kurus M; Hoffman G; Johnson J
[Ad] Endereço:Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, 333 Cedar Street, New Haven, Connecticut, USA.
[Ti] Título:Quantifying growing versus non-growing ovarian follicles in the mouse.
[So] Source:J Ovarian Res;10(1):3, 2017 Jan 13.
[Is] ISSN:1757-2215
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: A standard histomorphometric approach has been used for nearly 40 years that identifies atretic (e.g., dying) follicles by counting the number of pyknotic granulosa cells (GC) in the largest follicle cross-section. This method holds that if one pyknotic granulosa nucleus is seen in the largest cross section of a primary follicle, or three pyknotic cells are found in a larger follicle, it should be categorized as atretic. Many studies have used these criteria to estimate the fraction of atretic follicles that result from genetic manipulation or environmental insult. During an analysis of follicle development in a mouse model of Fragile X premutation, we asked whether these 'historical' criteria could correctly identify follicles that were not growing (and could thus confirmed to be dying). METHODS: Reasoning that the fraction of mitotic GC reveals whether the GC population was increasing at the time of sample fixation, we compared the number of pyknotic nuclei to the number of mitotic figures in follicles within a set of age-matched ovaries. RESULTS: We found that, by itself, pyknotic nuclei quantification resulted in high numbers of false positives (improperly categorized as atretic) and false negatives (improperly categorized intact). For preantral follicles, scoring mitotic and pyknotic GC nuclei allowed rapid, accurate identification of non-growing follicles with 98% accuracy. This method most often required the evaluation of one follicle section, and at most two serial follicle sections to correctly categorize follicle status. For antral follicles, we show that a rapid evaluation of follicle shape reveals which are intact and likely to survive to ovulation. CONCLUSIONS: Combined, these improved, non-arbitrary methods will greatly improve our ability to estimate the fractions of growing/intact and non-growing/atretic follicles in mouse ovaries.
[Mh] Termos MeSH primário: Atresia Folicular/fisiologia
Folículo Ovariano/fisiologia
[Mh] Termos MeSH secundário: Animais
Núcleo Celular/patologia
Modelos Animais de Doenças
Feminino
Síndrome do Cromossomo X Frágil/patologia
Células da Granulosa/citologia
Células da Granulosa/metabolismo
Células da Granulosa/patologia
Camundongos
Mitose
Folículo Ovariano/citologia
Folículo Ovariano/crescimento & desenvolvimento
Insuficiência Ovariana Primária/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170115
[St] Status:MEDLINE
[do] DOI:10.1186/s13048-016-0296-x


  8 / 801 MEDLINE  
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[PMID]:27664382
[Au] Autor:Donadeu FX; Mohammed BT; Ioannidis J
[Ad] Endereço:The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian EH25 9RG, UK. Electronic address: xavier.donadeu@roslin.ed.ac.uk.
[Ti] Título:A miRNA target network putatively involved in follicular atresia.
[So] Source:Domest Anim Endocrinol;58:76-83, 2017 Jan.
[Is] ISSN:1879-0054
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In a previous microarray study, we identified a subset of micro RNAS (miRNAs), which expression was distinctly higher in atretic than healthy follicles of cattle. In the present study, we investigated the involvement of those miRNAs in granulosa and theca cells during atresia. Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR) confirmed that miR-21-5p/-3p, miR-150, miR-409a, miR-142-5p, miR-378, miR-222, miR-155, and miR-199a-5p were expressed at higher levels in atretic than healthy follicles (9-17 mm, classified based on steroidogenic capacity). All miRNAs except miR-21-3p and miR-378 were expressed at higher levels in theca than granulosa cells. The expression of 13 predicted miRNA targets was determined in follicular cells by RT-qPCR, revealing downregulation of HIF1A, ETS1, JAG1, VEGFA, and MSH2 in either or both cell types during atresia. Based on increases in miRNA levels simultaneous with decreases in target levels in follicular cells, several predicted miRNA target interactions were confirmed that are putatively involved in follicular atresia, namely miR-199a-5p/miR-155-HIF1A in granulosa cells, miR-155/miR-222-ETS1 in theca cells, miR-199a-5p-JAG1 in theca cells, miR-199a-5p/miR-150/miR-378-VEGFA in granulosa and theca cells, and miR-155-MSH2 in theca cells. These results offer novel insight on the involvement of miRNAs in follicle development by identifying a miRNA target network that is putatively involved in follicle atresia.
[Mh] Termos MeSH primário: Bovinos
Atresia Folicular/genética
MicroRNAs/fisiologia
[Mh] Termos MeSH secundário: Animais
Bovinos/genética
Bovinos/fisiologia
Feminino
Expressão Gênica
Células da Granulosa/química
Células da Granulosa/metabolismo
MicroRNAs/genética
Análise em Microsséries/veterinária
Reprodutibilidade dos Testes
Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária
Células Tecais/química
Células Tecais/metabolismo
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE


  9 / 801 MEDLINE  
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[PMID]:27882941
[Au] Autor:Du X; Zhang L; Li X; Pan Z; Liu H; Li Q
[Ad] Endereço:College of Animal Science and Technology, Department of Animal Genetics, Breeding and Reproduction, Nanjing Agricultural University, Nanjing, China.
[Ti] Título:TGF-ß signaling controls FSHR signaling-reduced ovarian granulosa cell apoptosis through the SMAD4/miR-143 axis.
[So] Source:Cell Death Dis;7(11):e2476, 2016 Nov 24.
[Is] ISSN:2041-4889
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Follicle-stimulating hormone receptor (FSHR) and its intracellular signaling control mammalian follicular development and female infertility. Our previous study showed that FSHR is downregulated during follicular atresia of porcine ovaries. However, its role and regulation in follicular atresia remain unclear. Here, we showed that FSHR knockdown induced porcine granulosa cell (pGC) apoptosis and follicular atresia, and attenuated the levels of intracellular signaling molecules such as PKA, AKT and p-AKT. FSHR was identified as a target of miR-143, a microRNA that was upregulated during porcine follicular atresia. miR-143 enhanced pGC apoptosis by targeting FSHR, and reduced the levels of intracellular signaling molecules. SMAD4, the final molecule in transforming growth factor (TGF)-ß signaling, bound to the promoter and induced significant downregulation of miR-143 in vitro and in vivo. Activated TGF-ß signaling rescued miR-143-reduced FSHR and intracellular signaling molecules, and miR-143-induced pGC apoptosis. Overall, our findings offer evidence to explain how TGF-ß signaling influences and FSHR signaling for regulation of pGC apoptosis and follicular atresia by a specific microRNA, miR-143.
[Mh] Termos MeSH primário: Apoptose
Células da Granulosa/citologia
Células da Granulosa/metabolismo
MicroRNAs/metabolismo
Receptores do FSH/metabolismo
Transdução de Sinais
Proteína Smad4/metabolismo
Fator de Crescimento Transformador beta/metabolismo
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Feminino
Atresia Folicular/metabolismo
Técnicas de Silenciamento de Genes
Modelos Biológicos
Regiões Promotoras Genéticas/genética
Ligação Proteica/genética
Sus scrofa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (Receptors, FSH); 0 (Smad4 Protein); 0 (Transforming Growth Factor beta)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161125
[St] Status:MEDLINE
[do] DOI:10.1038/cddis.2016.379


  10 / 801 MEDLINE  
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[PMID]:27684714
[Au] Autor:Torres-Ramírez N; Escobar ML; Vázquez-Nin GH; Ortiz R; Echeverría OM
[Ad] Endereço:Departamento de Biología Celular, Laboratorio de Microscopía Electrónica, Facultad de Ciencias, Universidad Nacional Autónoma de México (UNAM), Av. Universidad 3000, Circuito Exterior S/N Delegación Coyoacán, C.P. Ciudad Universitaria, 04510, Ciudad de México, Mexico.
[Ti] Título:Paraptosis-like cell death in Wistar rat granulosa cells.
[So] Source:Dev Growth Differ;58(8):651-663, 2016 Oct.
[Is] ISSN:1440-169X
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Follicular atresia, a common process present in all mammals, involves apoptotic and autophagic cell death. However, the participation of paraptosis, a type of caspase-independent cell death, during follicular atresia is unknown. This study found swollen endoplasmic reticulum in the granulosa cells of adult Wistar rats. Calnexin was used as a marker of the endoplasmic reticulum at the ultrastructural and optical levels. The cells with swelling of the endoplasmic reticulum were negative to the TUNEL assay and active caspase-3 immunodetection, indicating that this swelling is not part of any apoptotic or autophagic process. Additionally, immunodetection of the CHOP protein was used as a marker of endoplasmic reticulum stress, and this confirmed the presence of the paraptosis process. These data suggest that paraptosis-like cell death is associated with the death of granulosa cells during follicular atresia in adult Wistar rats.
[Mh] Termos MeSH primário: Estresse do Retículo Endoplasmático
Retículo Endoplasmático/metabolismo
Atresia Folicular/metabolismo
Células da Granulosa/metabolismo
[Mh] Termos MeSH secundário: Animais
Calnexina/metabolismo
Caspase 3/metabolismo
Morte Celular
Feminino
Ratos
Ratos Wistar
Fator de Transcrição CHOP
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ddit3 protein, rat); 139873-08-8 (Calnexin); 147336-12-7 (Transcription Factor CHOP); EC 3.4.22.- (Casp3 protein, rat); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170209
[Lr] Data última revisão:
170209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160930
[St] Status:MEDLINE
[do] DOI:10.1111/dgd.12322



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