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[PMID]:29346447
[Au] Autor:Chang HJ; Shin HS; Kim TH; Yoo JY; Teasley HE; Zhao JJ; Ha UH; Jeong JW
[Ad] Endereço:Department of Obstetrics, Gynecology and Reproductive Biology, Michigan State University, Grand Rapids, MI, United States of America.
[Ti] Título:Pik3ca is required for mouse uterine gland development and pregnancy.
[So] Source:PLoS One;13(1):e0191433, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The PI3K/AKT signaling pathway plays a critical role in the maintenance of equilibrium between cell survival and apoptosis. The Pik3ca gene is mutated in a range of human cancers. It has been found to be oncogenic, and mutations lead to constitutive activation of the PI3K/AKT pathway. The expression patterns of PIK3CA proteins in the uterus of mice during early pregnancy indicate that it may play a role in the regulation of glandular epithelial cells, which is required to support uterine receptivity. To further investigate the role of Pik3ca in uterine function, Pik3ca was conditionally ablated only in the PGR-positive cells (Pgrcre/+Pik3caf/f; Pik3cad/d). A defect of uterine gland development and decidualization led to subfertility observed in Pik3cad/d mice. Pik3cad/d mice showed significantly decreased uterine weight compared to Pik3caf/f mice. Interestingly, a significant decrease of gland numbers were detected in Pik3cad/d mice compared to control mice. In addition, we found a decrease of Foxa2 expression, which is a known uterine gland marker in Pik3cad/d mice. Furthermore, the excessive proliferation of endometrial epithelial cells was observed in Pik3cad/d mice. Our studies suggest that Pik3ca has a critical role in uterine gland development and female fertility.
[Mh] Termos MeSH primário: Fosfatidilinositol 3-Quinases/metabolismo
Útero/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Western Blotting
Proliferação Celular
Decídua/citologia
Decídua/crescimento & desenvolvimento
Implantação do Embrião
Feminino
Fertilidade
Camundongos
Camundongos Endogâmicos C57BL
Mutação
Fosfatidilinositol 3-Quinases/genética
Gravidez
Reação em Cadeia da Polimerase em Tempo Real
Útero/citologia
Útero/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.1.137 (Pik3ca protein, mouse)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191433


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[PMID]:29325267
[Au] Autor:Shen JD; Wu W; Shu L; Cai LL; Xie JZ; Ma L; Sun XP; Cui YG; Liu JY
[Ad] Endereço:The Center of Reproductive Medicine, the First Affiliated Hospital of Nanjing Medical University, State Key Laboratory of Reproductive Medicine, Nanjing 210029, China.
[Ti] Título:[Analysis of clinical outcomes of different embryo stage biopsy in array comparative genomic hybridization based preimplantation genetic diagnosis and screening].
[So] Source:Zhonghua Fu Chan Ke Za Zhi;52(12):828-834, 2017 Dec 25.
[Is] ISSN:0529-567X
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To evaluate the efficiency of the application of array comparative genomic hybridization (array-CGH) in preimplantation genetic diagnosis or screening (PGD/PGS), and compare the clinical outcomes of different stage embryo biopsy. The outcomes of 381 PGD/PGS cycles referred in the First Affiliated Hospital of Nanjing Medical University from July 2011 to August 2015 were retrospectively analyzed. There were 320 PGD cycles with 156 cleavage-stage-biopsy cycles and 164 trophectoderm-biopsy cycles, 61 PGS cycles with 23 cleavage-stage-biopsy cycles and 38 trophectoderm-biopsy cycles. Chromosomal analysis was performed by array-CGH technology combined with whole genome amplification. Single embryo transfer was performed in all transfer cycles. Live birth rate was calculated as the main clinical outcomes. The embryo diagnosis rate of PGD/PGS by array-CGH were 96.9%-99.1%. In PGD biopsy cycles, the live birth rate per embryo transfer cycle and live birth rate per embryo biopsy cycle were 50.0%(58/116) and 37.2%(58/156) in cleavage-stage-biopsy group, 67.5%(85/126) and 51.8%(85/164) in trophectoderm-biopsy group (both 0.01). In PGS biopsy cycles, the live birth rate per embryo transfer cycle and live birth rate per embryo biopsy cycle were the same as 34.8%(8/23) in cleavage-stage-biopsy group, the same as 42.1%(16/38) in trophectoderm-biopsy group (both 0.05). High diagnosis rate and idea live birth rate are achieved in PGD/PGS cycles based on array-CGH technology. The live birth rate of trophectoderm-biopsy group is significantly higher than that of cleavage-stage-biopsy group in PGD cycles; the efficiency of trophectoderm-biopsy is better.
[Mh] Termos MeSH primário: Hibridização Genômica Comparativa
Implantação do Embrião/genética
Implantação do Embrião/fisiologia
Transferência Embrionária/métodos
Testes Genéticos
Diagnóstico Pré-Implantação/métodos
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Biópsia
Técnicas de Cultura Embrionária
Feminino
Seres Humanos
Gravidez
Estudos Retrospectivos
Transferência de Embrião Único
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0529-567x.2017.12.007


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[PMID]:28449669
[Au] Autor:Gleicher N; Metzger J; Croft G; Kushnir VA; Albertini DF; Barad DH
[Ad] Endereço:The Center for Human Reproduction, 21 East 69th Street, New York, NY, 10021, USA. ngleicher@thechr.com.
[Ti] Título:A single trophectoderm biopsy at blastocyst stage is mathematically unable to determine embryo ploidy accurately enough for clinical use.
[So] Source:Reprod Biol Endocrinol;15(1):33, 2017 Apr 27.
[Is] ISSN:1477-7827
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: It has become increasingly apparent that the trophectoderm (TE) at blastocyst stage is much more mosaic than has been appreciated. Whether preimplantation genetic screening (PGS), utilizing a single TE biopsy (TEB), can reliably determine embryo ploidy has, therefore, increasingly been questioned in parallel. METHODS: We for that reason here established 2 mathematical models to assess probabilities of false-negative and false-positive results of an on average 6-cell biopsy from an approximately 300-cell TE. This study was a collaborative effort between investigators at The Center for Human Reproduction in New York City and the Center for Studies in Physics and Biology and the Brivanlou Laboratory of Stem Cell Biology and Molecular Embryology, the latter two both at Rockefeller University in New York City. RESULTS: Both models revealed that even under best case scenario, assuming even distribution of mosaicism in TE (since mosaicism is usually clonal, a highly unlikely scenario), a biopsy of at least 27 TE cells would be required to reach minimal diagnostic predictability from a single TEB. CONCLUSIONS: As currently performed, a single TEB is, therefore, mathematically incapable of reliably determining whether an embryo can be transferred or should be discarded. Since a single TEB, as currently performed, apparently is not representative of the complete TE, this study, thus, raises additional concern about the clinical utilization of PGS.
[Mh] Termos MeSH primário: Blastocisto
Fase de Clivagem do Zigoto
Ectoderma/patologia
Ploidias
Diagnóstico Pré-Implantação/métodos
Trofoblastos/patologia
[Mh] Termos MeSH secundário: Aneuploidia
Biópsia
Blastocisto/metabolismo
Blastocisto/patologia
Fase de Clivagem do Zigoto/metabolismo
Fase de Clivagem do Zigoto/patologia
Implantação do Embrião/genética
Feminino
Seres Humanos
Modelos Teóricos
Gravidez
Diagnóstico Pré-Implantação/normas
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1186/s12958-017-0251-8


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[PMID]:29319820
[Au] Autor:Ashary N; Tiwari A; Modi D
[Ad] Endereço:Molecular and Cellular Biology Laboratory, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Mumbai, India.
[Ti] Título:Embryo Implantation: War in Times of Love.
[So] Source:Endocrinology;159(2):1188-1198, 2018 02 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Contrary to widespread belief, the implantation of an embryo for the initiation of pregnancy is like a battle, in that the embryo uses a variety of coercive tactics to force its acceptance by the endometrium. We propose that embryo implantation involves a three-step process: (1) identification of a receptive endometrium; (2) superimposition of a blastocyst-derived signature onto the receptive endometrium before implantation; and finally (3) breaching by the embryo and trophoblast invasion, culminating in decidualization and placentation. We review here the story that is beginning to emerge, focusing primarily on the cells that are in "combat" during this process.
[Mh] Termos MeSH primário: Implantação do Embrião/fisiologia
Placentação/fisiologia
[Mh] Termos MeSH secundário: Animais
Blastocisto/fisiologia
Embrião de Mamíferos
Feminino
Seres Humanos
Gravidez
Transdução de Sinais/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1210/en.2017-03082


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[PMID]:29244071
[Au] Autor:Muter J; Alam MT; Vrljicak P; Barros FSV; Ruane PT; Ewington LJ; Aplin JD; Westwood M; Brosens JJ
[Ad] Endereço:Division of Biomedical Sciences, Clinical Science Research Laboratories, Warwick Medical School, University of Warwick, Coventry, United Kingdom.
[Ti] Título:The Glycosyltransferase EOGT Regulates Adropin Expression in Decidualizing Human Endometrium.
[So] Source:Endocrinology;159(2):994-1004, 2018 02 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In pregnancy, resistance of endometrial decidual cells to stress signals is critical for the integrity of the fetomaternal interface and, by extension, survival of the conceptus. O-GlcNAcylation is an essential posttranslational modification that links glucose sensing to cellular stress resistance. Unexpectedly, decidualization of primary endometrial stromal cells (EnSCs) was associated with a 60% reduction in O-linked ß-N-acetylglucosamine (O-GlcNAc)‒modified proteins, reflecting downregulation of the enzyme that adds O-GlcNAc to substrates (O-GlcNAc transferase; OGT) but not the enzyme that removes the modification (O-GlcNAcase). Notably, epidermal growth factor domain-specific O-linked GlcNAc transferase (EOGT), an endoplasmic reticulum-specific OGT that modifies a limited number of secreted and membrane proteins, was markedly induced in differentiating EnSCs. Knockdown of EOGT perturbed a network of decidual genes involved in multiple cellular functions. The most downregulated gene upon EOGT knockdown in decidualizing cells was the energy homeostasis-associated gene (ENHO), which encodes adropin, a metabolic hormone involved in energy homeostasis and glucose and fatty acid metabolism. Analysis of midluteal endometrial biopsies revealed an inverse correlation between endometrial EOGT and ENHO expression and body mass index. Taken together, our findings revealed that obesity impairs the EOGT-adropin axis in decidual cells, which in turn points toward a mechanistic link between metabolic disorders and adverse pregnancy outcome.
[Mh] Termos MeSH primário: Proteínas Sanguíneas/genética
Implantação do Embrião/genética
Endométrio/metabolismo
N-Acetilglucosaminiltransferases/fisiologia
Peptídeos/genética
[Mh] Termos MeSH secundário: Biópsia
Proteínas Sanguíneas/metabolismo
Índice de Massa Corporal
Células Cultivadas
Endométrio/enzimologia
Endométrio/patologia
Feminino
Regulação da Expressão Gênica
Seres Humanos
Infertilidade Feminina/complicações
Infertilidade Feminina/genética
Infertilidade Feminina/patologia
Obesidade/complicações
Obesidade/genética
Obesidade/patologia
Peptídeos/metabolismo
Gravidez
Complicações na Gravidez/genética
Complicações na Gravidez/patologia
Resultado da Gravidez/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Blood Proteins); 0 (Enho protein, human); 0 (Peptides); EC 2.4.1.- (EOGT protein, human); EC 2.4.1.- (N-Acetylglucosaminyltransferases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1210/en.2017-03064


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[PMID]:29287711
[Au] Autor:Li W; Xi Y; Xue S; Wang Y; Wu L; Liu H; Lei M
[Ad] Endereço:Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education and Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, PR China.
[Ti] Título:Sequence analysis of microRNAs during pre-implantation between Meishan and Yorkshire pigs.
[So] Source:Gene;646:20-27, 2018 Mar 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Embryonic implantation in sows is a coordinated interaction between the implantation-competent blastocyst and receptive uterus. In addition, microRNAs are small endogenous non-coding RNAs which are involved in post-transcriptional gene regulation of several biological processes including embryonic implantation. However, the mechanisms of miRNAs involved in embryonic implantation of sows remain largely unknown. Here, we analyzed miRNAome of endometrium on day 9, 12 and 15 of pregnancy and on day 12 of non-pregnancy in Meishan and Yorkshire pigs by Illumina sequencing. From 24 libraries, we identified 312 known microRNAs and 211 potential novel miRNAs. Bioinformatics analysis showed that differentially expressed microRNAs on day 12 of pregnancy between the two breeds may play critical roles by involving "p53 signaling pathway" and "Wnt signaling pathway". Furthermore, our results demonstrated that ssc-miR-21, ssc-miR-451, ssc-miR-204, ssc-miR-199a-5p and ssc-miR-199b-5p would play crucial roles for implantation. The data generated in this study were expected to elucidate the influence of microRNAs during pre-implantation in pigs.
[Mh] Termos MeSH primário: Implantação do Embrião
Sequenciamento de Nucleotídeos em Larga Escala/métodos
MicroRNAs/genética
Análise de Sequência de RNA/métodos
Sus scrofa/genética
[Mh] Termos MeSH secundário: Animais
Biologia Computacional/métodos
Endométrio/química
Feminino
Perfilação da Expressão Gênica/veterinária
Regulação da Expressão Gênica no Desenvolvimento
Redes Reguladoras de Genes
Idade Gestacional
Sequenciamento de Nucleotídeos em Larga Escala/veterinária
Gravidez
Análise de Sequência de RNA/veterinária
Transdução de Sinais
Sus scrofa/classificação
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


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[PMID]:28451773
[Au] Autor:Chen X; Saravelos SH; Liu Y; Huang J; Wang CC; Li TC
[Ad] Endereço:Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Shatin, Hong Kong.
[Ti] Título:Correlation between three-dimensional power Doppler and morphometric measurement of endometrial vascularity at the time of embryo implantation in women with unexplained recurrent miscarriage.
[So] Source:J Mol Histol;48(3):235-242, 2017 Jun.
[Is] ISSN:1567-2387
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Power Doppler in combination with three-dimensional (3D-PD) ultrasonography has been used as a noninvasive tool to evaluate the vascularity. However, it is unclear whether 3D-PD can accurately reflect endometrial vascularization and replace the invasive endometrial biopsy. This study aims to investigate the correlation between 3D-PD and micro vessel morphometric measurement of endometrial vascularity. Twenty-five women with unexplained recurrent miscarriage were recruited for 3D-PD and endometrial biopsy on precisely day LH + 7. Immunohistochemistry using vWF was employed to identify micro vessels in endometrial biopsy specimens followed by the use of morphometric technique to measure the mean vessel diameter and volume fractions. The vascularization index (VI), flow index (FI) and vascularization flow index (VFI) assessed by 3D-PD were calculated for both the endometrial and sub-endometrial regions. There were no significant correlations between any of the ultrasonographic measurements (endometrial thickness, endometrial volume, endometrial VI/FI/VFI, sub-endometrial volume, sub-endometrial VI/FI/VFI) and morphometric features (number of micro vessel, mean diameter of micro vessel and volume fraction measurement of vessel). This study indicates that endometrial vascularity assessed by 3D-PD could not be used to reflect changes in micro vessels of the endometrium at the time of embryo implantation in women with unexplained recurrent miscarriage.
[Mh] Termos MeSH primário: Aborto Habitual/etiologia
Implantação do Embrião
Endométrio/irrigação sanguínea
Imagem Tridimensional/métodos
[Mh] Termos MeSH secundário: Adulto
Endométrio/diagnóstico por imagem
Feminino
Seres Humanos
Imagem Tridimensional/instrumentação
Microcirculação
Ultrassonografia Doppler em Cores
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1007/s10735-017-9722-7


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[PMID]:28466234
[Au] Autor:Negrón-Pérez VM; Hansen PJ
[Ad] Endereço:Department of Animal Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program and Genetics Institute, University of Florida, Gainesville, Florida, USA.
[Ti] Título:The bovine embryo hatches from the zona pellucida through either the embryonic or abembryonic pole.
[So] Source:J Assist Reprod Genet;34(6):725-731, 2017 Jun.
[Is] ISSN:1573-7330
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Implantation of the mammalian embryo in the uterus is preceded by escape from the zona pellucida. In some species, hatching from the zona occurs preferentially from one or the other poles of the embryo. The situation for the bovine embryo, in which hatching precedes attachment to the uterus by more than a week, is unclear. The purpose was to describe whether hatching of the bovine embryo from the zona pellucida occurs preferentially from the embryonic or abembryonic pole. METHODS: Bovine blastocysts undergoing hatching were examined by light microscopy (n = 84) and epifluorescence imaging using antibodies for markers of epiblast, hypoblast, and trophectoderm (TE) (n = 26). The location of hatching was classified as being at the embryonic pole, if hatching occurred ipsilateral to the inner cell mass (ICM), or abembryonic, if hatching occurred contralateral to the ICM. RESULTS: A total of 55% of blastocysts exited the zona pellucida through an opening at the embryonic pole. In these cases, 68% of the cells emerging through the zona pellucida were derived from the ICM. The remainder of blastocysts hatched from an opening either contralateral or to the side of the ICM. In these cases, 87% of hatched cells were TE. CONCLUSION: For the bovine embryo, there is nearly equal probability of hatching from the embryonic or abembryonic poles. Given that the surface area of the zona pellucida in contact with the TE overlying the ICM is less than for the remainder of the blastocyst, there is some preference for hatching through the embryonic pole. Thus, the bovine embryo is distinct from the mouse and human, where hatching occurs preferentially at the abembryonic pole.
[Mh] Termos MeSH primário: Implantação do Embrião/fisiologia
Desenvolvimento Embrionário/fisiologia
Útero/crescimento & desenvolvimento
Zona Pelúcida/fisiologia
[Mh] Termos MeSH secundário: Animais
Blastocisto/fisiologia
Bovinos
Contagem de Células
Implantação do Embrião/genética
Embrião de Mamíferos/fisiologia
Desenvolvimento Embrionário/genética
Feminino
Seres Humanos
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1007/s10815-017-0933-3


  9 / 10287 MEDLINE  
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[PMID]:29196267
[Au] Autor:Kusama K; Nakamura K; Bai R; Nagaoka K; Sakurai T; Imakawa K
[Ad] Endereço:Animal Resource Science Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Ibaraki, 319-0206, Japan.
[Ti] Título:Intrauterine exosomes are required for bovine conceptus implantation.
[So] Source:Biochem Biophys Res Commun;495(1):1370-1375, 2018 01 01.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Exosomes, extracellular vesicles, are present in uterine flushing fluids (UFs), which are involved in conceptus-endometrial interactions during peri-implantation periods. Despite several studies on intrauterine exosomes conducted, the roles conceptus and endometrial exosomes play during peri-implantation periods have not been well characterized. To investigate the effect of bovine intrauterine exosomes on conceptus implantation, exosomes isolated from bovine UFs during peri-implantation periods were subjected to global protein analysis. The analysis detected 596 exosomal proteins, including ruminants' pregnancy recognition factor IFNT, and 172 differentially expressed proteins with more than 1.5-fold changes in UFs on days 17, 20 and 22 pregnancy (day of conceptus implantation is initiated on days 19-19.5). Treatment of primary bovine endometrial epithelial cells with exosomes from day 17 UFs up-regulated the expression of apoptosis-related genes, and treatment with exosomes from day 20 and 22 UFs up-regulated the expression of adhesion molecule. Based on these findings, intrauterine exosomes should be considered as an essential constituent for successful implantation.
[Mh] Termos MeSH primário: Implantação do Embrião/fisiologia
Exossomos/metabolismo
Prenhez/fisiologia
Proteoma/metabolismo
Útero/fisiologia
Útero/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Bovinos
Exossomos/ultraestrutura
Feminino
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Proteome)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171203
[St] Status:MEDLINE


  10 / 10287 MEDLINE  
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[PMID]:29216287
[Au] Autor:Huang J; Jin N; Qin H; Shi X; Liu Y; Cheung W; Wang CC; Chan TF; Li TC
[Ad] Endereço:Department of Obstetrics and Gynaecology, the Chinese University of Hong Kong, Hong Kong SAR, China.
[Ti] Título:Transcriptomic profiles in peripheral blood between women with unexplained recurrent implantation failure and recurrent miscarriage and the correlation with endometrium: A pilot study.
[So] Source:PLoS One;12(12):e0189159, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AIM: To study the transcriptome profiles in the blood of recurrent implantation failure (RIF), recurrent miscarriage (RM) and fertile women during the window of implantation, and further analysis the correlation of transcriptome profiles between blood and endometrium. METHODS: This is an observational prospective study. In total 9 subjects were recruited, 3 RIF, 3 RM, and 3 controls. Paired samples (endometrium and peripheral blood) from the same subjects were precisely timed on the 7th days after luteal hormone surge (LH+7). RNA sequencing was applied to investigate the transcriptome profiles. RESULTS: The results of transcriptome in peripheral blood cannot be used to characterize women with RIF and unexplained RM. There was a medium level correlation between transcriptome in peripheral blood and endometrium during the window of implantation. CONCLUSION: The differential transcriptome patterns in blood are not representative of those in endometrium, and the blood transcriptome cannot differentiate among the women with RIF, RM or fertile.
[Mh] Termos MeSH primário: Aborto Habitual
Implantação do Embrião
Endométrio/patologia
Transcriptoma
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Projetos Piloto
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189159



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