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Pesquisa : G08.686.784.769.887 [Categoria DeCS]
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  1 / 2517 MEDLINE  
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[PMID]:28461656
[Au] Autor:Behringer R; Gertsenstein M; Nagy KV; Nagy A
[Ti] Título:Shipment of Live Preimplantation-Stage Mouse Embryos.
[So] Source:Cold Spring Harb Protoc;2017(5):pdb.prot092742, 2017 May 01.
[Is] ISSN:1559-6095
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sharing genetically modified mouse models is a very important part of collaboration between researchers. Shipping live animals around the world is inconvenient, expensive, and cumbersome because of the variety of international regulations and paperwork. The issue of health status differences between animal facilities is of great importance; traditionally, imported animals are quarantined to determine their health status and avoid the introduction of undesirable pathogens. The shipment of preimplantation-stage embryos for immediate transfer into pseudopregnant recipients upon arrival is a commonly used method for transportation. Time coordination on both sides is critical in this case, but the shipment can be done by any courier and the container does not need to be returned. This protocol has been used since the early 1990s to rederive dozens of mouse strains.
[Mh] Termos MeSH primário: Blastocisto/fisiologia
Camundongos/embriologia
Transportes/métodos
[Mh] Termos MeSH secundário: Animais
Transferência Embrionária
Feminino
Indicadores e Reagentes
Pseudogravidez
Transportes/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indicators and Reagents)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1101/pdb.prot092742


  2 / 2517 MEDLINE  
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[PMID]:28901587
[Au] Autor:Sattler R; Polasek L
[Ad] Endereço:Alaska Sea Life Center, Seward, Alaska.
[Ti] Título:Serum estradiol and progesterone profiles during estrus, pseudopregnancy, and active gestation in Steller sea lions.
[So] Source:Zoo Biol;36(5):323-331, 2017 Sep.
[Is] ISSN:1098-2361
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:While the proximate driver behind the decline of the Western stock of Steller sea lions (Eumetopias jubatus, >80% since 1970s) is likely multifactorial, the population reduction may have been powered by a decrease in fecundity. A harvest of Steller sea lions in the 1970s and 80s revealed a 30% reduction in the proportion of pregnant females from early (October-November) to late gestation (April-May). Identification and quantification of these reproductive failures are difficult when we lack species-specific data on endocrinology associated with discrete stages of the reproductive cycle (i.e., estrus, implantation, and gestation). We tracked changes in serum estradiol and progesterone in three adult female Steller sea lions from 2011 to 2015. In all years and most females, a discrete increase in estradiol was observed during the breeding season (June-August), indicative of estrus. Estradiol concentrations from October to May in a pregnant female compared to her corresponding values when non-pregnant did not consistently differ through gestation. An elevation in progesterone was observed in all females and all years beginning approximately in June and lasting through November. This likely results from progesterone production by the corpus luteum in both pregnant and pseudopregnant females. Serum progesterone shows promise as a diagnostic tool to identify pregnancy during months 3-5 (December-February) of the 8-month active gestation following embryonic implantation. This study provides ranges of key hormones during estrus, embryonic diapause/pseudopregnancy, and gestation in pregnant and non-pregnant females for studying reproduction in Steller sea lions.
[Mh] Termos MeSH primário: Estradiol/sangue
Estro/sangue
Prenhez
Progesterona/sangue
Leões-Marinhos/sangue
Leões-Marinhos/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Gravidez
Prenhez/sangue
Pseudogravidez/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
4G7DS2Q64Y (Progesterone); 4TI98Z838E (Estradiol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1002/zoo.21381


  3 / 2517 MEDLINE  
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[PMID]:28215431
[Au] Autor:Buska K; Kedzierska AE; Slawek A; Chelmonska-Soyta A
[Ad] Endereço:Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
[Ti] Título:Global decrease in the expression of signalling pathways' genes in murine uterus during preimplantation pregnancy.
[So] Source:Reprod Biol;17(1):89-96, 2017 Mar.
[Is] ISSN:2300-732X
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Early preimplantation embryo-maternal communication is crucial for the establishment and development of pregnancy. Though the involvement of several candidate genes and proteins in this complex event has been described, the hierarchy of molecular networks governing this communication remains unknown. The primary objective of this study was to determine whether the presence of embryos in the uterine lumen stimulated or inhibited gene expression in the uterine tissue on day 3.5 post coitum. To answer this question, we investigated the gene expression of dedicated signal transduction pathways in the uterus of CD-1 mice during the preimplantation stage of pregnancy and compared this expression to mice with induced pseudopregnancy. The expression levels of 84 genes assigned to nine intracellular signalling pathways were investigated by real-time PCR. The results demonstrated down-regulation of the uterine gene expression in the majority of pathways. Among target genes, 27 were significantly (p<0.05) down-regulated, and only three were significantly up-regulated. A majority of the down-regulated genes were found to be regulated by the TGFB and NFKB pathways, which suggests that the presence of the embryo selectively regulates signalling within signal transduction pathways. One of the up-regulated genes crucial for early pregnancy was Ptgs2 (p<0.05). The increased amount of both Ptgs2 gene and protein products indicates that Ptgs2 expression may be the earliest positive embryo signal for implantation and pregnancy recognition in mice. In conclusion, our results not only underline which signalling pathways are regulated in embryo-maternal communication before implantation but also support "the quiet state hypothesis" of silencing gene expression.
[Mh] Termos MeSH primário: Blastocisto/fisiologia
Ciclo-Oxigenase 2/metabolismo
Indução Enzimática
Regulação da Expressão Gênica no Desenvolvimento
Gravidez/metabolismo
Pseudogravidez/metabolismo
Útero/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais não Endogâmicos
Ciclo-Oxigenase 2/genética
Implantação do Embrião
Feminino
Perfilação da Expressão Gênica
Camundongos
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Transdução de Sinais
Organismos Livres de Patógenos Específicos
Útero/enzimologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.14.99.- (Ptgs2 protein, mouse); EC 1.14.99.1 (Cyclooxygenase 2)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


  4 / 2517 MEDLINE  
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[PMID]:28183999
[Au] Autor:Patterson AL; Pirochta J; Tufano SY; Teixeira JM
[Ad] Endereço:Department of ObstetricsGynecology, and Reproductive Biology, College of Human Medicine, Michigan State University, Grand Rapids, Michigan, USA.
[Ti] Título:Gain-of-function ß-catenin in the uterine mesenchyme leads to impaired implantation and decidualization.
[So] Source:J Endocrinol;233(1):119-130, 2017 Apr.
[Is] ISSN:1479-6805
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Embryo implantation and endometrial decidualization are critical events that occur during early pregnancy in humans and mice, and perturbation in either can result in infertility. WNT signaling through the canonical ß-catenin pathway plays a pivotal role in embryonic Müllerian duct development, postnatal uterine maturation and establishment of pregnancy. Loss of ß-catenin in the Müllerian duct mesenchyme (MDM)-derived stroma and myometrium results in impaired decidualization and infertility, whereas gain-of-function (GOF) results in the formation of mesenchymal tumors and sub-fertility attributed to malformed oviducts. We hypothesized that GOF ß-catenin further contributes to sub-fertility through improper stromal and epithelial cell signaling during embryo implantation and decidualization. We show that mice with GOF ß-catenin in MDM-derived stroma and myometrium have reduced implantation sites after embryo transfer and decreased decidualization. On day 4.5 of pseudopregnancy or in mice treated with progesterone and estrogen to mimic early pregnancy, the estrogen-LIF-ERK and progesterone-IHH pathways remain predominantly intact in GOF ß-catenin mice; however, JAK/STAT signaling is altered. pSTAT3 is significantly reduced in GOF ß-catenin mice and expression of downstream epithelial junctional complex factors, and , is increased. We also show that purified stromal cells from GOF ß-catenin uteri, when removed from epithelial cell influence and provided with the appropriate hormonal stimuli, are able to decidualize indicating that the cells are intrinsically capable of decidualization. Taken together, these results suggest that dysregulated ß-catenin activity in the stroma affects epithelial cell STAT3 signaling and ultimately embryo implantation and stromal decidualization.
[Mh] Termos MeSH primário: Decídua/metabolismo
Implantação do Embrião/fisiologia
Células Epiteliais/metabolismo
Transdução de Sinais/fisiologia
Útero/metabolismo
beta Catenina/metabolismo
[Mh] Termos MeSH secundário: Animais
Decídua/efeitos dos fármacos
Estradiol/farmacologia
Feminino
Camundongos
Camundongos Transgênicos
Fosforilação/efeitos dos fármacos
Progesterona/farmacologia
Pseudogravidez
Fator de Transcrição STAT3/metabolismo
Transdução de Sinais/efeitos dos fármacos
Útero/efeitos dos fármacos
beta Catenina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (STAT3 Transcription Factor); 0 (beta Catenin); 4G7DS2Q64Y (Progesterone); 4TI98Z838E (Estradiol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170616
[Lr] Data última revisão:
170616
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1530/JOE-16-0502


  5 / 2517 MEDLINE  
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[PMID]:27546215
[Au] Autor:Satoh H; Yoshio H; Kawaminami M; Kurusu S
[Ad] Endereço:Laboratory of Veterinary Physiology, Kitasato University School of Veterinary Medicine, Towada, Aomori 034-8628, Japan.
[Ti] Título:Type-dependent differences in Fas expression and phagocytes distribution in rat corpora lutea during natural regression: an immunohistochemical evidence.
[So] Source:J Vet Med Sci;78(12):1771-1777, 2017 Jan 10.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Though Fas/Fas ligand (FasL) system-dependent apoptosis is considered to be the primary form of cell death in regressing corpus luteum (CL), the cellular identity and regulation of expression of the ligand and receptor molecules are not fully understood. Here, we focused on immunohistochemical determination of Fas expression during natural regression with comparison of three different types of rat CLs. Detected Fas was in good spatial association with cleaved caspase-3 and FasL proteins and with macrophages and neutrophils. In CLs of the cycle and pseudopregnancy, Fas-positive cell types included large and small luteal (steroidogenic) cells and capillary endothelial cells mainly, and blood-derived immune cells occasionally. Fas signals were abundant at multiple focal inflammatory-like sites. In contrast, Fas signals in CL of pregnancy did not localize in steroidogenic cells, but almost exclusively in endothelial cells and granulocytes. The signals scattered evenly throughout the CL tissue as phagocytes also did. In all CLs types, the numbers of Fas-expressing cells increased transiently after functional inactivation and at the early phase of structural regression. This observation revealed spatio-temporally regulated expression of Fas that was highly associated with apoptotic and phagocytotic systems and type-dependent differences in Fas expression and phagocytes dynamics in naturally regressing CL of rats.
[Mh] Termos MeSH primário: Corpo Lúteo/citologia
Proteína Ligante Fas/metabolismo
Luteólise/fisiologia
Fagócitos/citologia
[Mh] Termos MeSH secundário: Animais
Caspase 3/metabolismo
Corpo Lúteo/metabolismo
Proteína Ligante Fas/genética
Feminino
Regulação da Expressão Gênica
Macrófagos/metabolismo
Neutrófilos/metabolismo
Fagócitos/metabolismo
Gravidez
Pseudogravidez/veterinária
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fas Ligand Protein); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160823
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.16-0199


  6 / 2517 MEDLINE  
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[PMID]:27756404
[Au] Autor:Brosnihan KB; M Pulgar V; Bharadwaj MS; Neves LA; Yamaleyeva LM
[Ad] Endereço:Hypertension and Vascular Research, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC, 27157-1032, USA. bbrosnih@wakehealth.edu.
[Ti] Título:Local intra-uterine Ang-(1-7) infusion attenuates PGE and 6-keto PGF in decidualized uterus of pseudopregnant rats.
[So] Source:Reprod Biol Endocrinol;14(1):68, 2016 Oct 18.
[Is] ISSN:1477-7827
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cyclooxygenase (COX)-derived prostanoids (PGE PGI ) are important contributors to the process of decidualization. Previous studies showed the presence of Ang-(1-7) in the primary and secondary decidualized zones of the implantation site at early pregnancy. Decreased concentrations of Ang-(1-7) were found in the decidualized uterus compared to the non-decidualized uterus of pseudopregnant rats, suggesting that low levels of Ang-(1-7) are required for successful decidualization at early pregnancy. METHODS: To understand the role of Ang-(1-7) in prostaglandin production in a decidualized uterus, induced by a bolus injection of sesame oil, Ang-(1-7) (24 µg/kg/h) or vehicle was then infused directly into the decidualized uterine horn using an osmotic minipump. The right horns were not injected or infused and served as non-decidualized uterine horns in both groups of animals. RESULTS: Decidualization increased PGE concentration in the uterus (0.53 ± 0.05 vs. 12.0 ± 3.2 pmol/mg protein, p < 0.001, non-decidualized vs. decidualized horns); Ang-(1-7) infusion attenuated the increase of PGE (12.0 ± 3.2 vs. 5.1 ± 1.3 pmol/mg protein, p < 0.01 control vs. Ang-(1-7) treated decidualized horns). The stable metabolite of PGI (6-keto PGF ) was increased with decidualization (0.79 ± 0.17 vs. 3.5 ± 0.82 pmol/mg protein, p < 0.001, non-decidualized vs. decidualized horns). Ang-(1-7) infusion attenuated the increase in 6-keto PGF in the decidualized horn (3.5 ± 0.82 vs 1.8 ± 0.37 pmol/mg protein, p < 0.05 control vs. Ang-(1-7) treated decidualized horns). The circulating levels of 6-keto-PGF and TXB were decreased by Ang-(1-7) infusion, while no difference was observed in circulating PGE . Although the global assessment of cleaved caspase 3 immunostaining, a marker of apoptosis, was unchanged within the Ang-(1-7) decidualized horn, there were localized decreases in cleaved caspase 3 staining in the luminal region in the decidualized uterus of Ang-(1-7)-treated rats. CONCLUSIONS: These studies show that increased local uterine Ang-(1-7) alters the uterine prostaglandin environment, possibly leading to disruptions of early events of decidualization.
[Mh] Termos MeSH primário: 6-Cetoprostaglandina F1 alfa/metabolismo
Angiotensina I/administração & dosagem
Dinoprostona/metabolismo
Fragmentos de Peptídeos/administração & dosagem
Pseudogravidez/metabolismo
Útero/metabolismo
[Mh] Termos MeSH secundário: 6-Cetoprostaglandina F1 alfa/antagonistas & inibidores
Animais
Decídua/efeitos dos fármacos
Decídua/metabolismo
Dinoprostona/antagonistas & inibidores
Feminino
Gravidez
Ratos
Ratos Sprague-Dawley
Útero/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptide Fragments); 58962-34-8 (6-Ketoprostaglandin F1 alpha); 9041-90-1 (Angiotensin I); IJ3FUK8MOF (angiotensin I (1-7)); K7Q1JQR04M (Dinoprostone)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE


  7 / 2517 MEDLINE  
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[PMID]:27717400
[Au] Autor:Xiong Y; Li W; Lin P; Wang L; Wang N; Chen F; Li X; Wang A; Jin Y
[Ad] Endereço:Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
[Ti] Título:Expression and regulation of ATF6α in the mouse uterus during embryo implantation.
[So] Source:Reprod Biol Endocrinol;14(1):65, 2016 Oct 07.
[Is] ISSN:1477-7827
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: ATF6α, one of the sensor proteins in the stress signaling pathway of the endoplasmic reticulum, is located in the membrane of the endoplasmic reticulum. To date, the physiological function of ATF6α in the process of embryo implantation has not been reported. METHODS: In this study, the expression pattern of ATF6α in the mouse uterus during peri-implantation and the estrous cycle was detected by real-time PCR, western blot and immunohistochemistry. RESULTS: ATF6α mRNA and protein levels were higher in the uterus near the implantation site on day 5 and were intensely expressed in the secondary decidual zone (SDZ) on days 7-8. In the uteri of pseudopregnant mice, ATF6α mRNA and protein levels were lower on day 5 than on other days. The activating blastocyst and artificial decidualization had an obvious effect of increasing the expression of ATF6α. In addition, the expression of ATF6α was affected by progesterone (P ) and estrogen (E ) in ovariectomized mice. This finding is further supported by evidence from mice during the estrous cycle. CONCLUSIONS: Thus, we have concluded that ATF6α may play an important role during embryo implantation and decidualization.
[Mh] Termos MeSH primário: Fator 6 Ativador da Transcrição/biossíntese
Implantação do Embrião/fisiologia
Regulação da Expressão Gênica no Desenvolvimento
Útero/metabolismo
[Mh] Termos MeSH secundário: Fator 6 Ativador da Transcrição/genética
Animais
Ciclo Estral/genética
Ciclo Estral/metabolismo
Feminino
Masculino
Camundongos
Gravidez
Pseudogravidez/genética
Pseudogravidez/metabolismo
RNA Mensageiro/biossíntese
RNA Mensageiro/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Activating Transcription Factor 6); 0 (Atf6 protein, mouse); 0 (RNA, Messenger)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170324
[Lr] Data última revisão:
170324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161009
[St] Status:MEDLINE


  8 / 2517 MEDLINE  
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[PMID]:27151362
[Au] Autor:Noguchi M; Ikedo T; Kawaguchi H; Tanimoto A
[Ad] Endereço:Department of Veterinary Medicine, Azabu University, Kanagawa 252-5201, Japan.
[Ti] Título:Estrus synchronization in microminipig using estradiol dipropionate and prostaglandin F2α.
[So] Source:J Reprod Dev;62(4):373-8, 2016 Aug 25.
[Is] ISSN:1348-4400
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The induction of pseudopregnancy by the exogenous administration of estradiol dipropionate (EDP) was investigated in cyclic Microminipigs (MMpigs) and the effects of exogenous administration of prostaglandin (PG) F2α on estrus exhibition were assessed in pseudopregnant MMpigs. In experiment 1, ovariectomized MMpigs were given a single intramuscular injection of 0.5, 1.5, or 2.5 mg of EDP. The estradiol-17ß level at each of these doses was significantly higher 1 to 3 days after EDP administration than on the day of the injection. In experiment 2, animals were given 1.5 mg of EDP once at 9 to 12 days after the end of estrus (D0) and then no (1.5 mg × 1 group), one (D0 and D4; 1.5 mg × 2 group), or two (D0, D4 and D7; 1.5 mg × 3 group) additional treatments. The pseudopregnancy rate was significantly higher in the 1.5 mg × 3 than in the 1.5 mg × 1 group. In experiment 3, PGF2α was administered twice between 26 and 28 days after EDP treatment to five pseudopregnant gilts with a 24-h interval between the two injections. Estrus after PGF2α treatment and LH surge were observed in 100% and 80% pseudopregnant MMpigs, respectively. The interval from the day of the first PGF2α treatment to the onset of estrus was 6.5 ± 0.2 days. These results indicate that multiple EDP treatments are required for induction of pseudopregnancy in MMpigs and estrus exhibition can be controlled in MMpigs by treatment with EDP and PGF2α.
[Mh] Termos MeSH primário: Dinoprosta/farmacologia
Estradiol/análogos & derivados
Sincronização do Estro/métodos
Pseudogravidez
[Mh] Termos MeSH secundário: Animais
Estradiol/farmacologia
Sincronização do Estro/efeitos dos fármacos
Feminino
Ovariectomia
Suínos
Porco Miniatura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
4TI98Z838E (Estradiol); B7IN85G1HY (Dinoprost); NIG5418BXB (estradiol dipropionate)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170518
[Lr] Data última revisão:
170518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160507
[St] Status:MEDLINE
[do] DOI:10.1262/jrd.2015-169


  9 / 2517 MEDLINE  
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[PMID]:27033606
[Au] Autor:Lang N; Wu B; He B; Wang L; Wang J
[Ad] Endereço:Graduate School of Peking Union Medical College, Beijing, People's Republic of China; Reproductive Physiology Laboratory, National Research Institute for Family Planning, Beijing, People's Republic of China.
[Ti] Título:Spontaneous decidualization in pseudopregnant rats with vitamin E deficiency.
[So] Source:Biochem Biophys Res Commun;473(4):828-833, 2016 05 13.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Successful implantation of an embryo requires adequate depth of invasion in the endometrium, which depends upon decidualization. The aim of the present study was to elucidate why humans experience spontaneous decidualization and menstruation while most other mammals do not. We established a spontaneous decidualization model in pseudopregnant rats with vitamin E deficiency (VED) to investigate mechanisms associated with spontaneous decidualization. Vaginal smears were used to monitor bleeding while vitamin E levels were analyzed with a commercial vitamin E assay kit. Trypan blue staining was used to observe the implantation site at 5.5 days post-coitum (dpc). Uterine morphology, estradiol (E2) and progesterone levels, and the anti-oxidation system were evaluated at 5.5, 7.5, and 9.5 dpc. The proportion of rats in the VED group exhibiting endometrial bleeding gradually increased (5.9%, 32.3%, and 50%) over three consecutive cycles of pseudopregnancy. Vitamin E levels in the VED group were markedly lower compared to the control group in both the plasma and uterus, while the level of vitamin E in the liver did not differ between the control and VED groups. Spontaneous decidualization in the VED group was validated by histological examination and immunohistochemistry. At 5.5 dpc, the mean serum E2 level in the VED group was more than twice that of the control group. The mean total anti-oxidizing capability, catalase level, and glutathione peroxidase activity were significantly reduced in the decidualized portion of the VED group compared to controls, while the malondialdehyde level was also significantly higher in the decidualized portion of the VED group. We hypothesize that the E2 surge at 5.5 dpc and increasing levels of reactive oxygen species are responsible for spontaneous decidualization in VED rats.
[Mh] Termos MeSH primário: Deciduoma/fisiopatologia
Estradiol/metabolismo
Complicações na Gravidez/metabolismo
Pseudogravidez/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Deficiência de Vitamina E/complicações
Deficiência de Vitamina E/metabolismo
[Mh] Termos MeSH secundário: Animais
Feminino
Gravidez
Pseudogravidez/complicações
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Reactive Oxygen Species); 4TI98Z838E (Estradiol)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171117
[Lr] Data última revisão:
171117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160402
[St] Status:MEDLINE


  10 / 2517 MEDLINE  
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[PMID]:26817987
[Au] Autor:Carter CL; Adams JK; Czarra JA; Coan PN
[Ad] Endereço:Office of Laboratory Animal Care, College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee;, Email: clcarter@utk.edu.
[Ti] Título:An Incidence of Pseudopregnancy Associated with the Social Enrichment of Rabbits (Oryctolagus cuniculi).
[So] Source:J Am Assoc Lab Anim Sci;55(1):98-9, 2016 Jan.
[Is] ISSN:1559-6109
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Here we describe a case of pseudopregnancy in a New Zealand White rabbit as a result of pair housing with an aggressive conspecific. Clinical signs included fur pulling and nest building that developed shortly after separation from the aggressor. An ovariohysterectomy was performed, and histopathologic findings support the diagnosis of pseudopregnancy. When introducing adult female rabbits to pair housing, stable pairs may be difficult to achieve because of the dominance-associated behavior that can occur as hierarchal relationships are formed. Does that are pair-housed after puberty should be monitored for aggressive behavior.
[Mh] Termos MeSH primário: Agressão
Comportamento Animal
Abrigo para Animais
Pseudogravidez/veterinária
Coelhos
[Mh] Termos MeSH secundário: Animais
Feminino
Incidência
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160129
[St] Status:MEDLINE



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