[PMID]: | 28566500 |
[Au] Autor: | Li J; Hatano R; Xu S; Wan L; Yang L; Weinstein AM; Palmer L; Wang T |
[Ad] Endereço: | Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut. |
[Ti] Título: | Gender difference in kidney electrolyte transport. I. Role of AT receptor in thiazide-sensitive Na -Cl cotransporter activity and expression in male and female mice. |
[So] Source: | Am J Physiol Renal Physiol;313(2):F505-F513, 2017 Aug 01. |
[Is] ISSN: | 1522-1466 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | We studied gender differences in Na -Cl cotransporter (NCC) activity and expression in wild-type (WT) and AT receptor knockout (KO) mice. In renal clearance experiments, urine volume (UV), glomerular filtration rate, absolute Na (E ) and K (E ), and fractional Na (FE ) and K excretion were measured and compared at peak changes after bolus intravenous injection of hydrochlorothiazide (HCTZ; 30 mg/kg). In WT, females responded more strongly than males to HCTZ, with larger fractional increases of UV (7.8- vs. 3.4-fold), E (11.7- vs. 5.7-fold), FE (7.9- vs. 4.9-fold), and E (2.8- vs. 1.4-fold). In contrast, there were no gender differences in the responses to the diuretic in KO mice; HCTZ produced greater effects on male KO than on WT but similar effects on females. In WT, total (tNCC) and phosphorylated (pNCC) NCC protein expressions were 1.8- and 4.6-fold higher in females compared with males ( < 0.05), consistent with the larger response to HCTZ. In KO mice, tNCC and pNCC increased significantly in males to levels not different from those in females. There were no gender differences in the expression of the Na /H exchanger (NHE3) in WT; NHE3 protein decreased to similar extents in male and female KO animals, suggesting AT -mediated NHE3 expression in proximal tubules. The resulting increase in delivery of NaCl to the distal nephron may underlie increased NCC expression and activity in mice lacking the AT receptor. |
[Mh] Termos MeSH primário: |
Angiotensina II/metabolismo Receptor Tipo 1 de Angiotensina/metabolismo Caracteres Sexuais Trocadores de Sódio-Hidrogênio/metabolismo
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[Mh] Termos MeSH secundário: |
Animais Diurese Feminino Hidroclorotiazida Rim/metabolismo Masculino Camundongos Knockout Natriurese Fenótipo Proteínas Serina-Treonina Quinases/metabolismo Receptor Tipo 1 de Angiotensina/genética Receptores de Droga/metabolismo Simportadores de Cloreto de Sódio/metabolismo Trocador 3 de Sódio-Hidrogênio Membro 3 da Família 12 de Carreador de Soluto/metabolismo
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Receptor, Angiotensin, Type 1); 0 (Receptors, Drug); 0 (Slc12a3 protein, mouse); 0 (Slc9a3 protein, mouse); 0 (Sodium Chloride Symporters); 0 (Sodium-Hydrogen Exchanger 3); 0 (Sodium-Hydrogen Exchangers); 0 (Solute Carrier Family 12, Member 3); 0 (thiazide receptor); 0J48LPH2TH (Hydrochlorothiazide); 11128-99-7 (Angiotensin II); EC 2.7.1.- (Prkwnk4 protein, mouse); EC 2.7.1.- (Stk39 protein, mouse); EC 2.7.11.1 (Protein-Serine-Threonine Kinases) |
[Em] Mês de entrada: | 1709 |
[Cu] Atualização por classe: | 171116 |
[Lr] Data última revisão:
| 171116 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170602 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1152/ajprenal.00087.2017 |
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