Base de dados : MEDLINE
Pesquisa : G12.122 [Categoria DeCS]
Referências encontradas : 22877 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 2288 ir para página                         

  1 / 22877 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29324815
[Au] Autor:Yerabham ASK; Müller-Schiffmann A; Ziehm T; Stadler A; Köber S; Indurkhya X; Marreiros R; Trossbach SV; Bradshaw NJ; Prikulis I; Willbold D; Weiergräber OH; Korth C
[Ad] Endereço:Department of Neuropathology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
[Ti] Título:Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein.
[So] Source:PLoS One;13(1):e0191162, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Accumulating evidence suggests an important role for the Disrupted-in-Schizophrenia 1 (DISC1) protein in neurodevelopment and chronic mental illness. In particular, the C-terminal 300 amino acids of DISC1 have been found to mediate important protein-protein interactions and to harbor functionally important phosphorylation sites and disease-associated polymorphisms. However, long disordered regions and oligomer-forming subdomains have so far impeded structural analysis. VHH domains derived from camelid heavy chain only antibodies are minimal antigen binding modules with appreciable solubility and stability, which makes them well suited for the stabilizing proteins prior to structural investigation. Here, we report on the generation of a VHH domain derived from an immunized Lama glama, displaying high affinity for the human DISC1 C region (aa 691-836), and its characterization by surface plasmon resonance, size exclusion chromatography and immunological techniques. The VHH-DISC1 (C region) complex was also used for structural investigation by small angle X-ray scattering analysis. In combination with molecular modeling, these data support predictions regarding the three-dimensional fold of this DISC1 segment as well as its steric arrangement in complex with our VHH antibody.
[Mh] Termos MeSH primário: Camelídeos Americanos/imunologia
Proteínas do Tecido Nervoso/imunologia
Anticorpos de Cadeia Única/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Complexo Antígeno-Anticorpo/química
Complexo Antígeno-Anticorpo/genética
Reações Antígeno-Anticorpo
Fenômenos Biofísicos
Camelídeos Americanos/genética
Mapeamento de Epitopos
Feminino
Seres Humanos
Cadeias Pesadas de Imunoglobulinas/química
Cadeias Pesadas de Imunoglobulinas/genética
Camundongos
Modelos Moleculares
Proteínas do Tecido Nervoso/química
Proteínas do Tecido Nervoso/genética
Fragmentos de Peptídeos/química
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/imunologia
Domínios e Motivos de Interação entre Proteínas
Espalhamento a Baixo Ângulo
Anticorpos de Cadeia Única/genética
Ressonância de Plasmônio de Superfície
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigen-Antibody Complex); 0 (DISC1 protein, human); 0 (Disc1 protein, mouse); 0 (Immunoglobulin Heavy Chains); 0 (Nerve Tissue Proteins); 0 (Peptide Fragments); 0 (Single-Chain Antibodies)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191162


  2 / 22877 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28465032
[Au] Autor:Kamat V; Rafique A
[Ad] Endereço:Biomolecular HTS Center, Therapeutic Proteins, Regeneron Pharmaceuticals, 777, Old Saw Mill River Road, Tarrytown, NY, 10591, USA. Electronic address: vishal.kamat@regeneron.com.
[Ti] Título:Extending the throughput of Biacore 4000 biosensor to accelerate kinetic analysis of antibody-antigen interaction.
[So] Source:Anal Biochem;530:75-86, 2017 08 01.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The surface plasmon resonance (SPR) biosensors are being routinely used in different stages of drug discovery and development. However, the lack of high throughput SPR biosensors continues to be a primary bottleneck for the rapid kinetic screening of large panels of monoclonal antibodies (mAbs). To further increase the throughput of the Biacore 4000 biosensor, we have developed three kinetic screening assays to characterize mAb-antigen interactions - (i) 16-mAb capture kinetic, (ii) single cycle kinetic (SCK), and (iii) parallel kinetic (PK). The performance of all three kinetic assays was evaluated by characterizing the binding of kinetically diverse human mAbs to four antigens with molecular weights of 14kD, 29kD, 38kD, and 48kD and binding affinities ranging from 130pM to 200 nM. The binding rate constants measured using all three kinetic assays were reproducible across multiple experiments and correlated with the values generated using the conventional 8-mAb capture kinetic assay on the Biacore 4000 (R > 0.94). Moreover, the 16-mAb capture assay decreased experiment time and analyte consumption by 35% and 50%, respectively. This work illustrates the significance of the 16-mAb capture kinetic, SCK, and PK assays to increase the throughput of Biacore 4000 and to support rapid kinetic screening of mAbs.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/análise
Reações Antígeno-Anticorpo/fisiologia
Antígenos/imunologia
Técnicas Biossensoriais/métodos
Processamento de Imagem Assistida por Computador/métodos
Ressonância de Plasmônio de Superfície/métodos
[Mh] Termos MeSH secundário: Anticorpos Monoclonais/imunologia
Seres Humanos
Cinética
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antigens)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  3 / 22877 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29235322
[Au] Autor:Bobrovnik SA; Demchenko MO; Komisarenko SV
[Ti] Título:Kinetic parameters of polyreactive immunoglobulins interaction with antigens in the presence of protamine.
[So] Source:Ukr Biochem J;88(3):29-35, 2016 May-Jun.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:The discovered earlier phenomenon of the enhancment of polyreactive immunoglobulines (PRIGs) binding to antigens in the presence of protamine and Tween 20 was investigated in more details. The comparative analysis of PRIGs reaction dynamics with immobilized antigen was provided. In addition, the rate constants for the reaction and the affinity constants of PRIGs-antigen binding in the presence or absence of optimal protamine concentration were determined. The rate constant of PRIGs-antigen reaction did not increase in the presence of protamine optimal concentration and was even reduced approximately twice. However, in the presence of protamine the concentration of reactive PRIGs molecules, that were able to interact with antigen, increased approximately 30 times, and this led to strong reaction rate increase. Protamine also influenced the affinity constant of PRIGs-antigen binding, which increased approximately three times. The suggestion was made that such protamine effect was due to its influence on the PRIGs molecules special structure, and, as a result of the conformational change PRIGs became able to bind more effectively to the antigens.
[Mh] Termos MeSH primário: Reações Antígeno-Anticorpo
Antígenos/química
Imunoglobulinas/química
Protaminas/química
Soroalbumina Bovina/química
[Mh] Termos MeSH secundário: Animais
Afinidade de Anticorpos
Especificidade de Anticorpos
Antígenos/imunologia
Bovinos
Ensaio de Imunoadsorção Enzimática
Proteínas Imobilizadas
Cinética
Camundongos
Polissorbatos/química
Salmão
Soroalbumina Bovina/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens); 0 (Immobilized Proteins); 0 (Immunoglobulins); 0 (Polysorbates); 0 (Protamines); 27432CM55Q (Serum Albumin, Bovine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.03.029


  4 / 22877 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28748255
[Au] Autor:Katsumata O; Mori M; Sawane Y; Niimura T; Ito A; Okamoto H; Fukaya M; Sakagami H
[Ad] Endereço:Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa, 252-0374, Japan.
[Ti] Título:Cellular and subcellular localization of ADP-ribosylation factor 6 in mouse peripheral tissues.
[So] Source:Histochem Cell Biol;148(6):577-596, 2017 Dec.
[Is] ISSN:1432-119X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:ADP-ribosylation factor 6 (Arf6) is a small GTPase that regulates endosomal trafficking and actin cytoskeleton remodeling. In the present study, we comprehensively examined the cellular and subcellular localization of Arf6 in adult mouse peripheral tissues by immunofluorescence and immunoelectron microscopy using the heat-induced antigen retrieval method with Tris-EDTA buffer (pH 9.0). Marked immunolabeling of Arf6 was observed particularly in epithelial cells of several tissues including the esophagus, stomach, small and large intestines, trachea, kidney, epididymis, oviduct, and uterus. In most epithelial cells of simple or pseudostratified epithelia, Arf6 exhibited predominant localization to the basolateral membrane and a subpopulation of endosomes. At an electron microscopic level, Arf6 was localized along the basolateral membrane, with dense accumulation at interdigitating processes and infoldings. Arf6 was present in a ring-like appearance at intercellular bridges in spermatogonia and spermatocytes in the testis and at the Flemming body of cytokinetic somatic cells in the ovarian follicle, thymus, and spleen. The present study provides anatomical clues to help understand the physiological roles of Arf6 at the whole animal level.
[Mh] Termos MeSH primário: Fatores de Ribosilação do ADP/análise
Epididimo/química
Intestino Delgado/química
Rim/química
Oviductos/química
Testículo/química
[Mh] Termos MeSH secundário: Animais
Reações Antígeno-Anticorpo
Feminino
Imunofluorescência
Células HeLa
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos ICR
Microscopia Imunoeletrônica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.6.5.2 (ADP-Ribosylation Factors); EC 3.6.5.2 (ADP-ribosylation factor 6)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171206
[Lr] Data última revisão:
171206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1007/s00418-017-1599-8


  5 / 22877 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28791742
[Au] Autor:Mahmud MN; Oda M; Usui D; Inoshima Y; Ishiguro N; Kamatari YO
[Ad] Endereço:The United Graduate School of Veterinary Sciences, Gifu University, Gifu, 501-1193, Japan.
[Ti] Título:A multispecific monoclonal antibody G2 recognizes at least three completely different epitope sequences with high affinity.
[So] Source:Protein Sci;26(11):2162-2169, 2017 Nov.
[Is] ISSN:1469-896X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A monoclonal antibody (mAb) G2 possesses an unusual characteristic of reacting with at least three proteins (ATP6V1C1, SEPT3, and C6H10orf76) other than its original antigen, chicken prion protein (ChPrP). The epitopes on ChPrP and ATP6V1C1 have been identified previously. In this study, we identified the epitope in the third protein, SEPT3. Interestingly, there was no amino acid sequence similarity among the epitopes on the three proteins. These epitopes had high binding affinities to G2 (K = ∼10 M for monovalent binding and K = ∼10 M for divalent binding), as determined using a SPR biosensor. This is the first report on a three-in-one mAb recognizing completely different epitope sequences with high affinity. Additionally, competitive ELISA indicated that the binding sites on G2, specific for the three different epitopes, overlapped, suggesting that the antigen-binding site may be flexible in the free form and capable of adapting to at least three different conformations to enable interactions with three different antigens.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/metabolismo
Epitopos/química
Proteínas Nucleares/química
Proteínas Priônicas/química
Septinas/química
ATPases Vacuolares Próton-Translocadoras/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Anticorpos Monoclonais/biossíntese
Anticorpos Monoclonais/química
Afinidade de Anticorpos
Especificidade de Anticorpos
Reações Antígeno-Anticorpo
Sítios de Ligação
Ligação Competitiva
Galinhas
Clonagem Molecular
Ensaio de Imunoadsorção Enzimática
Mapeamento de Epitopos
Epitopos/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Cinética
Proteínas Nucleares/genética
Proteínas Nucleares/imunologia
Proteínas Priônicas/genética
Proteínas Priônicas/imunologia
Ligação Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Septinas/genética
Septinas/imunologia
Ressonância de Plasmônio de Superfície
ATPases Vacuolares Próton-Translocadoras/genética
ATPases Vacuolares Próton-Translocadoras/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Epitopes); 0 (Nuclear Proteins); 0 (Prion Proteins); 0 (Recombinant Proteins); EC 3.6.1.- (Septins); EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1002/pro.3263


  6 / 22877 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28782517
[Au] Autor:Suárez CF; Pabón L; Barrera A; Aza-Conde J; Patarroyo MA; Patarroyo ME
[Ad] Endereço:Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá D.C., Colombia; Universidad del Rosario, Bogotá D.C., Colombia; Universidad de Ciencias Aplicadas y Ambientales (UDCA), Bogotá, Colombia.
[Ti] Título:Structural analysis of owl monkey MHC-DR shows that fully-protective malaria vaccine components can be readily used in humans.
[So] Source:Biochem Biophys Res Commun;491(4):1062-1069, 2017 Sep 30.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:More than 50 years ago the owl monkey (genus Aotus) was found to be highly susceptible to developing human malaria, making it an excellent experimental model for this disease. Microbes and parasites' (especially malaria) tremendous genetic variability became resolved during our malaria vaccine development, involving conserved peptides having high host cell binding activity (cHABPs); however, cHABPs are immunologically silent and must be specially modified (mHABPs) to induce a perfect fit into major histocompatibility complex (MHC) molecules (HLA in humans). Since malarial immunity is mainly antibody-mediated and controlled by the HLA-DRB genetic region, ∼1000 Aotus have been molecularly characterised for MHC-DRB, revealing striking similarity between human and Aotus MHC-DRB repertories. Such convergence suggested that a large group of immune protection-inducing protein structures (IMPIPS), highly immunogenic and protection inducers against malarial intravenous challenge in Aotus, could easily be used in humans for inducing full protection against malaria. We highlight the value of a logical and rational methodology for developing a vaccine in an appropriate animal model: Aotus monkeys.
[Mh] Termos MeSH primário: Antígenos de Histocompatibilidade Classe II/química
Antígenos de Histocompatibilidade Classe II/imunologia
Vacinas Antimaláricas/química
Vacinas Antimaláricas/imunologia
[Mh] Termos MeSH secundário: Animais
Reações Antígeno-Anticorpo
Aotidae
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Histocompatibility Antigens Class II); 0 (Malaria Vaccines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE


  7 / 22877 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28755586
[Au] Autor:Attallah C; Aguilar MF; Garay AS; Herrera FE; Etcheverrigaray M; Oggero M; Rodrigues DE
[Ad] Endereço:UNL, CONICET, FBCB, Cell Culture Laboratory, Ciudad Universitaria UNL, Pje. "El Pozo" - C.C. 242, S3000ZAA Santa Fe, Argentina.
[Ti] Título:An unusual cysteine V 87 affects the antibody fragment conformations without interfering with the disulfide bond formation.
[So] Source:Mol Immunol;90:143-149, 2017 Oct.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Cys residues are almost perfectly conserved in all antibodies. They contribute significantly to the antibody fragment stability. The relevance of two natural contiguous Cys residues of an anti-recombinant human-follicle stimulation hormone (rhFSH) in a format of single-chain variable fragment (scFv) was studied. This scFv contains 5 Cys residues: V 22 and V 92 in the variable heavy chain (V ) and V 23, V 87 and V 88 in the variable light chain (V ). The influence of two unusual contiguous Cys at positions V 87 and V 88 was studied by considering the wild type fragment and mutant variants: V -C88S, V -C87S, V -C87Y. The analysis was carried out using antigen-binding ability measurement by indirect specific ELISA and a detailed molecular modeling that comprises homology methods, long molecular dynamics simulations and docking. We found that V -C87 affected the antibody fragment stability without interfering with the disulfide bond formation. The effect of mutating the V -C87 by a usual residue at this position like Tyr caused distant structural changes at the V region that confers a higher mobility to the V -CDR2 and V -CDR3 loops improving the scFv binding to the antigen.
[Mh] Termos MeSH primário: Cisteína/química
Hormônio Foliculoestimulante Humano/imunologia
Região Variável de Imunoglobulina/imunologia
Simulação de Acoplamento Molecular
Simulação de Dinâmica Molecular
Anticorpos de Cadeia Única/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Afinidade de Anticorpos/genética
Afinidade de Anticorpos/imunologia
Reações Antígeno-Anticorpo/imunologia
Ensaio de Imunoadsorção Enzimática
Seres Humanos
Cadeias Pesadas de Imunoglobulinas/química
Cadeias Pesadas de Imunoglobulinas/imunologia
Cadeias Leves de Imunoglobulina/química
Cadeias Leves de Imunoglobulina/imunologia
Região Variável de Imunoglobulina/química
Conformação Molecular
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Follicle Stimulating Hormone, Human); 0 (Immunoglobulin Heavy Chains); 0 (Immunoglobulin Light Chains); 0 (Immunoglobulin Variable Region); 0 (Single-Chain Antibodies); K848JZ4886 (Cysteine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170730
[St] Status:MEDLINE


  8 / 22877 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28720505
[Au] Autor:Patterson JT; Gros E; Zhou H; Atassi G; Kerwin L; Carmody L; Zhu T; Jones B; Fu Y; Kaufmann GF
[Ad] Endereço:Sorrento Therapeutics, Inc., 4955 Directors Place, San Diego, CA 92121, USA. Electronic address: jpatterson@sorrentotherapeutics.com.
[Ti] Título:Chemically generated IgG2 bispecific antibodies through disulfide bridging.
[So] Source:Bioorg Med Chem Lett;27(16):3647-3652, 2017 08 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bispecific antibodies (BsAbs) are designed to engage two antigens simultaneously, thus, effectively expanding the ability of antibody-based therapeutics to target multiple pathways within the same cell, engage two separate soluble antigens, bind the same antigen with distinct paratopes, or crosslink two different cell types. Many recombinant BsAb formats have emerged, however, expression and purification of such constructs can often be challenging. To this end, we have developed a chemical strategy for generating BsAbs using native IgG2 architecture. Full-length antibodies can be conjugated via disulfide bridging with linkers bearing orthogonal groups to produce BsAbs. We report that an αHER2/EGFR BsAb was successfully generated by this approach and retained the ability to bind both antigens with no significant loss of potency.
[Mh] Termos MeSH primário: Anticorpos Biespecíficos/química
Dissulfetos/química
Imunoglobulina G/imunologia
[Mh] Termos MeSH secundário: Anticorpos Biespecíficos/imunologia
Reações Antígeno-Anticorpo
Sítios de Ligação de Anticorpos
Linhagem Celular Tumoral
Química Click
Seres Humanos
Células MCF-7
Microscopia de Fluorescência
Receptor do Fator de Crescimento Epidérmico/imunologia
Receptor do Fator de Crescimento Epidérmico/metabolismo
Receptor ErbB-2/imunologia
Receptor ErbB-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bispecific); 0 (Disulfides); 0 (Immunoglobulin G); EC 2.7.10.1 (Receptor, Epidermal Growth Factor); EC 2.7.10.1 (Receptor, ErbB-2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170720
[St] Status:MEDLINE


  9 / 22877 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28698001
[Au] Autor:Markwalter CF; Jang IK; Burton RA; Domingo GJ; Wright DW
[Ad] Endereço:Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.
[Ti] Título:Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2.
[So] Source:Anal Biochem;534:10-13, 2017 Oct 01.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Predicting antibody pair performance in a sandwich format streamlines development of antibody-based diagnostics and laboratory research tools, such as enzyme-linked immunosorbent assays (ELISAs) and lateral flow immunoassays (LFAs). We have evaluated panels of monoclonal antibodies against the malarial parasite biomarker Plasmodium falciparum histidine rich protein 2 (HRP2), including 9 new monoclonal antibodies, using biolayer interferometry (BLI) and screened antibody pairs in a checkerboard ELISA. This study showed BLI predicts antibody pair ELISA performance for HRP2. Pairs that included capture antibodies with low off-rate constants and detection antibodies with high on-rate constants performed best in an ELISA format.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/imunologia
Antígenos de Protozoários/análise
Ensaio de Imunoadsorção Enzimática
Plasmodium falciparum/química
Proteínas de Protozoários/análise
[Mh] Termos MeSH secundário: Reações Antígeno-Anticorpo
Antígenos de Protozoários/imunologia
Plasmodium falciparum/imunologia
Proteínas de Protozoários/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antigens, Protozoan); 0 (HRP-2 antigen, Plasmodium falciparum); 0 (Protozoan Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171001
[Lr] Data última revisão:
171001
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE


  10 / 22877 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28669727
[Au] Autor:Usui D; Inaba S; Kamatari YO; Ishiguro N; Oda M
[Ad] Endereço:Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, Kyoto 606-8522, Japan.
[Ti] Título:Light-chain residue 95 is critical for antigen binding and multispecificity of monoclonal antibody G2.
[So] Source:Biochem Biophys Res Commun;490(4):1205-1209, 2017 Sep 02.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The monoclonal antibody, G2, specifically binds to the immunogen peptide derived from the chicken prion protein, Pep18mer, and two chicken proteins derived peptides, Pep8 and Pep395; G2 binds with equal affinity to Pep18mer. The amino acid sequences of the three peptides are completely different, and so the recognition mechanism of G2 is unique and interesting. We generated a single-chain Fv (scFv) antibody of G2, and demonstrated its correct folding with an antigen binding function similar to intact G2 antibody. We also generated a Pro containing mutant of G2 scFv at residue 95 of the light chain, and analyzed its antigen binding using a surface plasmon biosensor. The mutant lost its binding ability to Pep18mer, but remained those to Pep8 and Pep395. The results clearly indicate residue 95 as being critical for multispecific antigen binding of G2 at the site generated from the junctional diversity introduced at the joints between the V and J gene segments.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/imunologia
Antígenos/imunologia
Cadeias Leves de Imunoglobulina/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Anticorpos Monoclonais/genética
Reações Antígeno-Anticorpo
Antígenos/genética
Sítios de Ligação
Cadeias Leves de Imunoglobulina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antigens); 0 (Immunoglobulin Light Chains)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE



página 1 de 2288 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde