Base de dados : MEDLINE
Pesquisa : G15.649 [Categoria DeCS]
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[PMID]:29031775
[Au] Autor:Qiu Y; Liao L; Jin X; Mao D; Liu R
[Ad] Endereço:Hunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, School of Life Science, Hunan Normal University, Changsha 410081, China.
[Ti] Título:Analysis of the meiotic transcriptome reveals the genes related to the regulation of pollen abortion in cytoplasmic male-sterile pepper (Capsicum annuum L.).
[So] Source:Gene;641:8-17, 2018 Jan 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:CMS, which refers to the inability to generate functional pollen grains while still producing a normal gynoecium, has been widely used for pepper hybrid seed production. Pepper line 8214A is an excellent CMS line exhibiting 100% male sterility and superior economic characteristics. A TUNEL assay revealed the nuclear DNA is damaged in 8214A PMCs during meiosis. TEM images indicated that the 8214A PMCs exhibited asynchronous meiosis after prophase I, and some PMCs degraded prematurely with morphological features typical of PCD. Additionally, at the end of meiosis, the 8214A PMCs formed abnormal non-tetrahedral tetrads that degraded in situ. To identify the genes involved in the pollen abortion of line 8214A, the transcriptional profiles of the 8214A and the 8214B anthers (i.e., from the fertile maintainer line) during meiosis were analyzed using an RNA-seq approach. A total of 1355 genes were determined to be differentially expressed, including 424 and 931 up- and down- regulated genes, respectively, in the 8214A anthers during meiosis relative to the expression levels in the 8214B. The expression levels of ubiquitin ligase and cell cycle-related genes were apparently down-regulated, while the expression of methyltransferase genes was up-regulated in the 8214A anthers during meiosis, which likely contributed to the PCD of these PMCs during meiosis. Thus, our results may be useful for revealing the molecular mechanism regulating the pollen abortion of CMS pepper.
[Mh] Termos MeSH primário: Capsicum/genética
Proteínas de Ciclo Celular/metabolismo
Meiose/genética
Metiltransferases/metabolismo
Infertilidade das Plantas/genética
Pólen/genética
[Mh] Termos MeSH secundário: Proteínas de Ciclo Celular/genética
Dano ao DNA/genética
Flores/metabolismo
Perfilação da Expressão Gênica
Sequenciamento de Nucleotídeos em Larga Escala
Meiose/fisiologia
Metiltransferases/genética
Transcriptoma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Cycle Proteins); EC 2.1.1.- (Methyltransferases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE


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[PMID]:28835997
[Au] Autor:Lukaszewski AJ
[Ad] Endereço:Department of Botany and Plant Sci., University of California, Riverside, CA, 92521, USA. adam.lukaszewski@ucr.edu.
[Ti] Título:Chromosomes 1BS and 1RS for control of male fertility in wheats and triticales with cytoplasms of Aegilops kotschyi, Ae. mutica and Ae. uniaristata.
[So] Source:Theor Appl Genet;130(12):2521-2526, 2017 Dec.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Engineered chromosomes 1BS and 1RS offer a new alternative in the development of hybrid systems in bread wheat and triticale. In the cytoplasmic male sterility system for hybrid wheat based on the cytoplasm of Triticum timopheevi fertility restoration is difficult, with few good restorer genes available. In the system based on the cytoplasms of Aegilops kotschyi, Ae. uniaristata and Ae. mutica, essentially all chromosomes 1B carry locus Rf that restores male fertility; male sterility manifests itself in wheats with the 1RS.1BL translocation where 1BS chromosome arm is missing. To generate male sterile wheats without the 1RS.1BL translocation, the 1BS arm was cytogenetically engineered to replace the segment with Rf with two short inserts of rye chromatin. Conversely, to enhance fertility restoration by doubling the number of restorers present for eventual use in wheat and triticale, a region of 1BS with Rf was inserted into 1RS. Alloplasmic wheats with Rf removed were completely male sterile; alloplasmic wheats with engineered 1RS carrying Rf and without normal 1B were male fertile. An exception to the ubiquitous presence of Rf is T. spelta var. duhamelianum; four accessions tested in this study gave inconsistent results but some did not restore male fertility. Engineered chromosomes 1BS and 1RS and chromosomes 1B of T. spelta offer a new alternative for practical application of a cytoplasmic male sterility system in the development of hybrid wheat and hexaploid triticale.
[Mh] Termos MeSH primário: Citoplasma/genética
Infertilidade das Plantas/genética
Triticale/genética
Triticum/genética
[Mh] Termos MeSH secundário: Cromossomos de Plantas
Engenharia Genética
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/fisiologia
Poliploidia
Translocação Genética
Triticale/fisiologia
Triticum/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170825
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2973-6


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[PMID]:28815278
[Au] Autor:Pucci A; Picarella ME; Mazzucato A
[Ad] Endereço:Department of Agricultural and Forestry Sciences (DAFNE), University of Tuscia, Via S.C. de Lellis snc, 01100, Viterbo, Italy.
[Ti] Título:Phenotypic, genetic and molecular characterization of 7B-1, a conditional male-sterile mutant in tomato.
[So] Source:Theor Appl Genet;130(11):2361-2374, 2017 Nov.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: We characterized the photoperiod-sensitive 7B - 1 male-sterile mutant in tomato, showing its allelism with stamenless - 2 . Mapping experiments indicated SlGLO2 , a B-class MADS-box family member, as a strong candidate to underlie the 7B - 1 mutation. The interest in male sterility (MS) dates back to a long time due to its perspective use in hybrid seed production. Here, we characterize 7B-1, a photoperiod-sensitive male-sterile (ms) mutant in tomato (Solanum lycopersicum L.), in which stamens are restored to fertility by permissive growth conditions in short days (SD). This system represents a useful strategy to facilitate the maintenance of the ms line. Examination of 7B-1 and other structural mutants, vms, sl, sl-2 and tap3, showed carpellization of stamens in the third floral whorl. 7B-1 exhibits strong expressivity in long days (LD), producing 100% aberrant anthers and virtually no seed production under open pollination, whereas it recovered fertility in SD. By genetic analysis, we demonstrate that 7B-1 is not allelic to sl nor to vms; instead it shows allelism to sl-2. Because the homeotic phenotype of the mutation resembles lesions to members of the B-class MADS-box transcription factor family, that specify petal and stamen identity, we pursued a candidate gene approach towards these targets. Using an interspecific backcross mapping population and markers linked to B-class MADS-box genes, significant linkage was found between 7B-1 and the SlGLO2 gene on Chr6. This result was supported by the 7B-1 phenotype that is similar to that of SlGLO2 knock outs and by the strong downregulation of the gene in the mutant. Although the lesion underlying the mutant phenotype is still elusive, our results pave the way for the final demonstration that SlGLO2 underlies 7B-1 and further the use of 7B-1 mutant in tomato hybrid seed production schemes.
[Mh] Termos MeSH primário: Lycopersicon esculentum/genética
Infertilidade das Plantas/genética
[Mh] Termos MeSH secundário: Alelos
Mapeamento Cromossômico
Cruzamentos Genéticos
Flores/genética
Ligação Genética
Marcadores Genéticos
Lycopersicon esculentum/fisiologia
Mutação
Fenótipo
Pólen/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2964-7


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[PMID]:28657614
[Au] Autor:Chen H; Zhao Z; Liu L; Kong W; Lin Y; You S; Bai W; Xiao Y; Zheng H; Jiang L; Li J; Zhou J; Tao D; Wan J
[Ad] Endereço:National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, China.
[Ti] Título:Genetic analysis of a hybrid sterility gene that causes both pollen and embryo sac sterility in hybrids between Oryza sativa L. and Oryza longistaminata.
[So] Source:Heredity (Edinb);119(3):166-173, 2017 Sep.
[Is] ISSN:1365-2540
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Oryza longistaminata originates from African wild rice and contains valuable traits conferring tolerance to biotic and abiotic stress. However, interspecific crosses between O. longistaminata and Oryza sativa cultivars are hindered by reproductive barriers. To dissect the mechanism of interspecific hybrid sterility, we developed a near-isogenic line (NIL) using indica variety RD23 as the recipient parent and O. longistaminata as the donor parent. Both pollen and embryo sac semi-sterility were observed in F hybrids between RD23 and NIL. Cytological analysis demonstrated that pollen abortion in F hybrids occurred at the early bi-nucleate stage due to a failure of the first mitosis in microspores. Partial embryo sacs in the F hybrids were defective during the functional megaspore formation stage. Most notably, nearly half of the male or female gametes were aborted in heterozygotes S40 S40 , regardless of their genotypes. Thus, S40 was indicated as a one-locus sporophytic sterility gene controlling both male and female fertility in hybrids between RD23 and O. longistaminata. A population of 16 802 plants derived from the hybrid RD23/NIL-S40 was developed to fine-map S40. Finally, the S40 locus was delimited to an 80-kb region on the short arm of chromosome 1 in terms with reference sequences of cv. 93-11. Eight open reading frames (ORFs) were localized in this region. On the basis of gene expression and genomic sequence analysis, ORF5 and ORF8 were identified as candidate genes for the S40 locus. These results are helpful in cloning the S40 gene and marker-assisted transferring of the corresponding neutral allele in rice breeding programs.
[Mh] Termos MeSH primário: Hibridização Genética
Oryza/genética
Infertilidade das Plantas/genética
Pólen/genética
[Mh] Termos MeSH secundário: Mapeamento Cromossômico
Cruzamentos Genéticos
DNA de Plantas/genética
Genes de Plantas
Marcadores Genéticos
Genótipo
Oryza/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (Genetic Markers)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1038/hdy.2017.32


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[PMID]:28604770
[Au] Autor:Guerrero RF; Muir CD; Josway S; Moyle LC
[Ad] Endereço:Department of Biology, Indiana University, Bloomington, Indiana, United States of America.
[Ti] Título:Pervasive antagonistic interactions among hybrid incompatibility loci.
[So] Source:PLoS Genet;13(6):e1006817, 2017 Jun.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Species barriers, expressed as hybrid inviability and sterility, are often due to epistatic interactions between divergent loci from two lineages. Theoretical models indicate that the strength, direction, and complexity of these genetic interactions can strongly affect the expression of interspecific reproductive isolation and the rates at which new species evolve. Nonetheless, empirical analyses have not quantified the frequency with which loci are involved in interactions affecting hybrid fitness, and whether these loci predominantly interact synergistically or antagonistically, or preferentially involve loci that have strong individual effects on hybrid fitness. We systematically examined the prevalence of interactions between pairs of short chromosomal regions from one species (Solanum habrochaites) co-introgressed into a heterospecific genetic background (Solanum lycopersicum), using lines containing pairwise combinations of 15 chromosomal segments from S. habrochaites in the background of S. lycopersicum (i.e., 95 double introgression lines). We compared the strength of hybrid incompatibility (either pollen sterility or seed sterility) expressed in each double introgression line to the expected additive effect of its two component single introgressions. We found that epistasis was common among co-introgressed regions. Interactions for hybrid dysfunction were substantially more prevalent in pollen fertility compared to seed fertility phenotypes, and were overwhelmingly antagonistic (i.e., double hybrids were less unfit than expected from additive single introgression effects). This pervasive antagonism is expected to attenuate the rate at which hybrid infertility accumulates among lineages over time (i.e., giving diminishing returns as more reproductive isolation loci accumulate), as well as decouple patterns of accumulation of sterility loci and hybrid incompatibility phenotypes. This decoupling effect might explain observed differences between pollen and seed fertility in their fit to theoretical predictions of the accumulation of isolation loci, including the 'snowball' effect.
[Mh] Termos MeSH primário: Cromossomos de Plantas/genética
Epistasia Genética
Lycopersicon esculentum/genética
Locos de Características Quantitativas/genética
Isolamento Reprodutivo
[Mh] Termos MeSH secundário: Mapeamento Cromossômico
Fertilidade/genética
Genótipo
Hibridização Genética
Lycopersicon esculentum/crescimento & desenvolvimento
Modelos Genéticos
Fenótipo
Infertilidade das Plantas/genética
Pólen/genética
Sementes/genética
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006817


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[PMID]:28520767
[Au] Autor:Singh M; Kumar M; Thilges K; Cho MJ; Cigan AM
[Ad] Endereço:DuPont Pioneer, Johnston, Iowa, United States of America.
[Ti] Título:MS26/CYP704B is required for anther and pollen wall development in bread wheat (Triticum aestivum L.) and combining mutations in all three homeologs causes male sterility.
[So] Source:PLoS One;12(5):e0177632, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Development of anthers and pollen represents an important aspect of the life cycle in flowering plants. Genes contributing to anther and pollen development have been widely studied in many plant species. Ms26/CYP704B genes play an important role in pollen development through biosynthesis of sporopollenin for pollen exine formation. To investigate the role of Ms26/CYP704B genes in anther and pollen development of bread wheat, mutations in the A-, B-, and D-homeologs of the putative Ms26/CYP704B gene were analyzed. Single and double homozygous mutants in any of the homeologs did not affect pollen development and male fertility. Triple homozygous mutants resulted in completely male sterile plants that were defective in pollen and anther development. Additionally, double homozygous-single heterozygous mutants were also male sterile although with varying levels of residual fertility. The fertility of these triple mutants was dependent upon the homeolog contributing the wild-type allele. Two heterologous Ms26/CYP704B genes, when transformed into a triple homozygous mutant background, completely restored male fertility, whereas a single gene was unable to restore fertility. Functional analysis of Ms26/CYP704B furthers the understanding of male fertility genes which can be utilized for the development of novel hybrid seed production systems in wheat.
[Mh] Termos MeSH primário: Sistema Enzimático do Citocromo P-450/genética
Flores/genética
Mutação
Infertilidade das Plantas/genética
Pólen/genética
Triticum/fisiologia
[Mh] Termos MeSH secundário: Alelos
Sequência de Aminoácidos
Sistema Enzimático do Citocromo P-450/química
Sistema Enzimático do Citocromo P-450/metabolismo
Regulação da Expressão Gênica de Plantas
Genes Recessivos
Teste de Complementação Genética
Proteínas de Plantas/química
Proteínas de Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177632


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[PMID]:28405714
[Au] Autor:Ji JL; Yang LM; Fang ZY; Zhuang M; Zhang YY; Lv HH; Liu YM; Li ZS
[Ad] Endereço:Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
[Ti] Título:Recessive male sterility in cabbage (Brassica oleracea var. capitata) caused by loss of function of BoCYP704B1 due to the insertion of a LTR-retrotransposon.
[So] Source:Theor Appl Genet;130(7):1441-1451, 2017 Jul.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: The LTR-retrotransposon insertion in BoCYP704B1 is proved to be the primary cause of the male sterility in cabbage. Effective allele-specific markers were developed for marker-assisted selection of male sterile gene. 83121A is a spontaneous male sterile mutant identified from cabbage. Genetic analysis indicated that male sterility is controlled by a single recessive gene. Pollen wall formation in the 83121A mutant was severely defective, with a lack of sporopollenin or exine. To understand the mechanisms of male sterility in 83121A, transcription analysis using RNA-Seq was carried out in the buds of the male sterile line 83121A and the male fertile line 83121B, which are near-isogenic lines differing only in the fertility trait. Via expression analysis of differentially expressed genes involved in pollen exine development before the bicellular pollen stage, BoCYP704B1 was identified as a candidate gene, which was approximately downregulated 30-fold in 83121A. BoCYP704B1 is a member of the evolutionarily conserved CYP704B family, which is essential for sporopollenin formation. The BoCYP704B1 transcript is specifically detected in the developing anthers of wild-type cabbage. Further sequence analysis revealed that a 5424-bp long terminal repeat-retrotransposon (LTR-RT) was inserted into the first exon of BoCYP704B1 in 83121A, which is not found in wild-type plants. The insertion of LTR-RT not only reduced the expression of BoCYP704B1 but also altered structure of protein encoded by BoCYP704B1. Moreover, linkage analysis showed that the homozygotic mutational BoCYP704B1 always cosegregated with male sterility. These data suggest that the LTR-RT insertion in BoCYP704B1 hinders sporopollenin formation in 83121A leading to male sterility. The allele-specific markers developed in this study were effective for marker-assisted selection of the male sterile gene.
[Mh] Termos MeSH primário: Brassica/genética
Sistema Enzimático do Citocromo P-450/genética
Genes Recessivos
Infertilidade das Plantas/genética
Retroelementos
[Mh] Termos MeSH secundário: Sequência de Bases
Brassica/fisiologia
Genes de Plantas
Marcadores Genéticos
Fenótipo
Pólen/genética
Pólen/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers); 0 (Retroelements); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2899-z


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[PMID]:28326434
[Au] Autor:Würschum T; Leiser WL; Weissmann S; Maurer HP
[Ad] Endereço:State Plant Breeding Institute, University of Hohenheim, 70593, Stuttgart, Germany. tobias.wuerschum@uni-hohenheim.de.
[Ti] Título:Genetic architecture of male fertility restoration of Triticum timopheevii cytoplasm and fine-mapping of the major restorer locus Rf3 on chromosome 1B.
[So] Source:Theor Appl Genet;130(6):1253-1266, 2017 Jun.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Restoration of fertility in the cytoplasmic male sterility-inducing Triticum timopheevii cytoplasm can be achieved with the major restorer locus Rf3 located on chromosome 1B, but is also dependent on modifier loci. Hybrid breeding relies on a hybrid mechanism enabling a cost-efficient hybrid seed production. In wheat and triticale, cytoplasmic male sterility based on the T. timopheevii cytoplasm is commonly used, and the aim of this study was to dissect the genetic architecture underlying fertility restoration. Our study was based on two segregating F triticale populations with 313 and 188 individuals that share a common female parent and have two different lines with high fertility restoration ability as male parents. The plants were cloned to enable replicated assessments of their phenotype and fertility restoration was evaluated based on seed set or staining for pollen fertility. The traits showed high heritabilities but their distributions differed between the two populations. In one population, a quarter of the lines were sterile, conforming to a 3:1 segregation ratio. QTL mapping identified two and three QTL in these populations, with the major QTL being detected on chromosome 1B. This QTL was collinear in both populations and likely corresponds to Rf3. We found that Rf3 explained approximately 30 and 50% of the genotypic variance, has a dominant mode of inheritance, and that the female parent lacks this locus, probably due to a 1B.1R translocation. Taken together, Rf3 is a major restorer locus that enables fertility restoration of the T. timopheevii cytoplasm, but additional modifier loci are needed for full restoration of male fertility. Consequently, Rf3 holds great potential for hybrid wheat and triticale breeding, but other loci must also be considered, either through marker-assisted or phenotypic selection.
[Mh] Termos MeSH primário: Mapeamento Cromossômico
Genes de Plantas
Infertilidade das Plantas/genética
Locos de Características Quantitativas
Triticum/genética
[Mh] Termos MeSH secundário: Genes Dominantes
Genótipo
Fenótipo
Pólen/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2885-5


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[PMID]:28315925
[Au] Autor:Hackauf B; Bauer E; Korzun V; Miedaner T
[Ad] Endereço:Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Rudolf-Schick-Platz 3a, 18190, Groß Lüsewitz, Germany. bernd.hackauf@julius-kuehn.de.
[Ti] Título:Fine mapping of the restorer gene Rfp3 from an Iranian primitive rye (Secale cereale L.).
[So] Source:Theor Appl Genet;130(6):1179-1189, 2017 Jun.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: A comparative genetics approach allowed to precisely determine the map position of the restorer gene Rfp3 in rye and revealed that Rfp3 and the restorer gene Rfm1 in barley reside at different positions in a syntenic 4RL/6HS segment. Cytoplasmic male sterility (CMS) is a reliable and striking genetic mechanism for hybrid seed production. Breeding of CMS-based hybrids in cereals requires the use of effective restorer genes as an indispensable pre-requisite. We report on the fine mapping of a restorer gene for the Pampa cytoplasm in winter rye that has been tapped from the Iranian primitive rye population Altevogt 14160. For this purpose, we have mapped 41 gene-derived markers to a 38.8 cM segment in the distal part of the long arm of chromosome 4R, which carries the restorer gene. Male fertility restoration was comprehensively analyzed in progenies of crosses between a male-sterile tester genotype and 21 recombinant as well as six non-recombinant BC S lines. This approach allowed us to validate the position of this restorer gene, which we have designated Rfp3, on chromosome 4RL. Rfp3 was mapped within a 2.5 cM interval and cosegregated with the EST-derived marker c28385. The gene-derived conserved ortholog set (COS) markers enabled us to investigate the orthology of restorer genes originating from different genetic resources of rye as well as barley. The observed localization of Rfp3 and Rfm1 in a syntenic 4RL/6HS segment asks for further efforts towards cloning of both restorer genes as an option to study the mechanisms of male sterility and fertility restoration in cereals.
[Mh] Termos MeSH primário: Mapeamento Cromossômico
Genes de Plantas
Infertilidade das Plantas/genética
Secale/genética
[Mh] Termos MeSH secundário: Cruzamentos Genéticos
Marcadores Genéticos
Irã (Geográfico)
Melhoramento Vegetal
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170320
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2879-3


  10 / 691 MEDLINE  
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[PMID]:28298376
[Au] Autor:Kamath A; Levin RA; Miller JS
[Ad] Endereço:Department of Biology, Amherst College, Amherst, Massachusetts 01002 USA.
[Ti] Título:Floral size and shape evolution following the transition to gender dimorphism.
[So] Source:Am J Bot;104(3):451-460, 2017 Mar.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Floral morphology is expected to evolve following the transition from cosexuality to gender dimorphism in plants, as selection through male and female function becomes dissociated. Specifically, male-biased dimorphism in flower size can arise through selection for larger flowers through male function, selection for smaller flowers through female function, or both. The evolutionary pathway to floral dimorphism can be most effectively reconstructed in species with intraspecific variation in sexual system. We examined the evolution of flower size and shape in , whose populations are either gender dimorphic with male and female plants, or cosexual with hermaphroditic plants. METHODS: Floral morphology was characterized in populations spanning the species' complete range. For a subset of the range where cosexual and dimorphic populations are in close proximity, we compared the size and shape of flowers from female and male plants in dimorphic populations to hermaphrodites in cosexual populations, accounting for variation associated with abiotic environmental conditions. KEY RESULTS: The magnitude of flower size dimorphism varied across dimorphic populations. After controlling for environmental variation across cosexual and dimorphic populations, flowers on males were larger than flowers on females and hermaphrodites, whereas flower size did not differ between females and hermaphrodites. Flower shape differences were associated with mating type, sexual system, and environmental variation. CONCLUSIONS: While abiotic environmental gradients shape both overall flower size and shape, male-biased flower size dimorphism in appears to arise through selection for larger flowers in males but not smaller flowers in females.
[Mh] Termos MeSH primário: Flores/genética
Lycium/genética
[Mh] Termos MeSH secundário: Evolução Biológica
Meio Ambiente
Flores/anatomia & histologia
Flores/crescimento & desenvolvimento
Flores/fisiologia
Geografia
Lycium/anatomia & histologia
Lycium/crescimento & desenvolvimento
Lycium/fisiologia
Infertilidade das Plantas
Reprodução
Caracteres Sexuais
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1600442



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