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  1 / 166 MEDLINE  
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[PMID]:28635965
[Au] Autor:Ma X; Cai Z; Liu W; Ge S; Tang L
[Ad] Endereço:College of Tropical Forestry, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, China.
[Ti] Título:Identification, genealogical structure and population genetics of S-alleles in Malus sieversii, the wild ancestor of domesticated apple.
[So] Source:Heredity (Edinb);119(3):185-196, 2017 Sep.
[Is] ISSN:1365-2540
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The self-incompatibility (SI) gene that is specifically expressed in pistils encodes the SI-associated ribonuclease (S-RNase), functioning as the female-specificity determinant of a gametophytic SI system. Despite extensive surveys in Malus domestica, the S-alleles have not been fully investigated for Malus sieversii, the primary wild ancestor of the domesticated apple. Here we screened the M. sieversii S-alleles via PCR amplification and sequencing, and identified 14 distinct alleles in this species. By contrast, nearly 40 are present in its close wild relative, Malus sylvestris. We further sequenced 8 nuclear genes to provide a neutral reference, and investigated the evolution of S-alleles via genealogical and population genetic analyses. Both shared ancestral polymorphism and an excess of non-synonymous substitution were detected in the S-RNases of the tribe Maleae in Rosaceae, indicating the action of long-term balancing selection. Approximate Bayesian Computations based on the reference neutral loci revealed a severe bottleneck in four of the six studied M. sieversii populations, suggesting that the low number of S-alleles found in this species is mainly the result of diversity loss due to a drastic population contraction. Such a bottleneck may lead to ambiguous footprints of ongoing balancing selection detected at the S-locus. This study not only elucidates the constituents and number of S-alleles in M. sieversii but also illustrates the potential utility of S-allele number shifts in demographic inference for self-incompatible plant species.
[Mh] Termos MeSH primário: Alelos
Genética Populacional
Malus/genética
Autoincompatibilidade em Angiospermas
[Mh] Termos MeSH secundário: Teorema de Bayes
DNA de Plantas/genética
Evolução Molecular
Frequência do Gene
Genes de Plantas
Variação Genética
Funções Verossimilhança
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.1038/hdy.2017.28


  2 / 166 MEDLINE  
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[PMID]:28441955
[Au] Autor:Muñoz-Sanz JV; Zuriaga E; López I; Badenes ML; Romero C
[Ad] Endereço:Fruit Tree Breeding Department, Instituto Valenciano de Investigaciones Agrarias (IVIA), CV-315, Km. 10,7., 46113, Moncada, Valencia, Spain.
[Ti] Título:Self-(in)compatibility in apricot germplasm is controlled by two major loci, S and M.
[So] Source:BMC Plant Biol;17(1):82, 2017 Apr 26.
[Is] ISSN:1471-2229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Apricot (Prunus armeniaca L.) exhibits a gametophytic self-incompatibility (GSI) system and it is mostly considered as a self-incompatible species though numerous self-compatible exceptions occur. These are mainly linked to the mutated S -haplotype carrying an insertion in the S-locus F-box gene that leads to a truncated protein. However, two S-locus unlinked pollen-part mutations (PPMs) termed m and m' have also been reported to confer self-compatibility (SC) in the apricot cultivars 'Canino' and 'Katy', respectively. This work was aimed to explore whether other additional mutations might explain SC in apricot as well. RESULTS: A set of 67 cultivars/accessions with different geographic origins were analyzed by PCR-screening of the S- and M-loci genotypes, contrasting results with the available phenotype data. Up to 20 S-alleles, including 3 new ones, were detected and sequence analysis revealed interesting synonymies and homonymies in particular with S-alleles found in Chinese cultivars. Haplotype analysis performed by genotyping and determining linkage-phases of 7 SSR markers, showed that the m and m' PPMs are linked to the same m haplotype. Results indicate that m -haplotype is tightly associated with SC in apricot germplasm being quite frequent in Europe and North-America. However, its prevalence is lower than that for S in terms of frequency and geographic distribution. Structures of 34 additional M-haplotypes were inferred and analyzed to depict phylogenetic relationships and M was found to be the closest haplotype to m Genotyping results showed that four cultivars classified as self-compatible do not have neither the S - nor the m -haplotype. CONCLUSIONS: According to apricot germplasm S-genotyping, a loss of genetic diversity affecting the S-locus has been produced probably due to crop dissemination. Genotyping and phenotyping data support that self-(in)compatibility in apricot relies mainly on the S- but also on the M-locus. Regarding this latter, we have shown that the m -haplotype associated with SC is shared by 'Canino', 'Katy' and many other cultivars. Its origin is still unknown but phylogenetic analysis supports that m arose later in time than S from a widely distributed M-haplotype. Lastly, other mutants putatively carrying new mutations conferring SC have also been identified deserving future research.
[Mh] Termos MeSH primário: Prunus armeniaca/genética
Autoincompatibilidade em Angiospermas/genética
[Mh] Termos MeSH secundário: Genótipo
Mutação
Filogeografia
Pólen/genética
Prunus armeniaca/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE
[do] DOI:10.1186/s12870-017-1027-1


  3 / 166 MEDLINE  
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[PMID]:28303339
[Au] Autor:Bittencourt Júnior NS
[Ad] Endereço:Departamento de Zoologia e Botânica, Instituto de Biociências Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho", Rua Cristóvão Colombo 2265, Jardim Nazareth, CEP 15054-000, São José do Rio Preto, São Paulo, Brazil. nesbitte@ibilce.unesp.br.
[Ti] Título:Evidence for post-zygotic self-incompatibility in Handroanthus impetiginosus (Bignoniaceae).
[So] Source:Plant Reprod;30(2):69-79, 2017 Jun.
[Is] ISSN:2194-7961
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Late-acting self-incompatibility (LSI) has been defined as a genetically controlled self-sterility mechanism that prevents seed set by selfing, despite normal pollen tube growth and ovule penetration in self-pollinated pistils. In species of the Bignoniaceae with LSI, such as Handroanthus impetiginosus, the selfed pistils are characterized by a marked delay in ovule penetration, fertilization, and endosperm initiation, followed by uniform pistil abscission. This highlights the contentious possibility of a post-zygotic self-incompatibility system. However, previous studies were unable to confirm fusion of the sperm and egg cell nuclei in selfed ovules. In the present study, the cytology of the embryo sac, double fertilization, and pistil longevity was investigated in H. impetiginosus using comparative nuclei microspectrofluorometry of DAPI-stained sections of self- vs. unpollinated pistils. Differences in both pistil longevity and ovary size between self- and unpollinated flowers at the time of pistil abscission were significant. Zygotes with double the DNA content in their nuclei relative to unfertilized egg cell nuclei were verified in selfed ovules from the first day after pollination onward, and G1 karyogamy appeared to have occurred. Our cytological analysis clearly indicates that ovules of self-pollinated pistils in H. impetiginosus are fertilized before pistil abscission but no embryogenesis initiation occurs, which strongly supports the idea of a post-zygotic self-incompatibility mechanism.
[Mh] Termos MeSH primário: Bignoniaceae/fisiologia
Autoincompatibilidade em Angiospermas
[Mh] Termos MeSH secundário: Bignoniaceae/genética
DNA de Plantas
Flores/fisiologia
Microespectrofotometria
Polinização
Autoincompatibilidade em Angiospermas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE
[do] DOI:10.1007/s00497-017-0300-7


  4 / 166 MEDLINE  
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[PMID]:28137595
[Au] Autor:Ma Y; Li Q; Hu G; Qin Y
[Ad] Endereço:State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
[Ti] Título:Comparative transcriptional survey between self-incompatibility and self-compatibility in Citrus reticulata Blanco.
[So] Source:Gene;609:52-61, 2017 Apr 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Seedlessness is an excellent economical trait, and self-incompatibility (SI) is one of important factors resulting in seedless fruit in Citrus. However, SI molecular mechanism in Citrus is still unclear. In this study, RNA-Seq technology was used to identify differentially expressed genes related to SI reaction of 'Wuzishatangju' (Citrus reticulata Blanco). A total of 35.67GB raw RNA-Seq data was generated and was de novo assembled into 50,364 unigenes with an average length of 897bp and N50 value of 1549. Twenty-three candidate unigenes related to SI were analyzed using qPCR at different tissues and stages after self- and cross-pollination. Seven pollen S genes (Unigene0050323, Unigene0001060, Unigene0004230, Unigene0004222, Unigene0012037, Unigene0048889 and Unigene0004272), three pistil S genes (Unigene0019191, Unigene0040115, Unigene0036542) and three genes (Unigene0038751, Unigene0031435 and Unigene0029897) associated with the pathway of ubiquitin-mediated proteolysis were identified. Unigene0031435, Unigene0038751 and Unigene0029897 are probably involved in SI reaction of 'Wuzishatangju' based on expression analyses. The present study provides a new insight into the molecular mechanism of SI in Citrus at the transcriptional level.
[Mh] Termos MeSH primário: Citrus/genética
Citrus/fisiologia
Proteínas de Plantas/genética
Autoincompatibilidade em Angiospermas
[Mh] Termos MeSH secundário: Flores/fisiologia
Pólen/fisiologia
RNA de Plantas/genética
Análise de Sequência de RNA
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (RNA, Plant)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE


  5 / 166 MEDLINE  
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[PMID]:28062510
[Au] Autor:Spigler RB
[Ad] Endereço:Department of Biology, Temple University, 1900 N. 12th Street, Philadelphia, PA 19122, USA rachel.spigler@temple.edu.
[Ti] Título:Plasticity of floral longevity and floral display in the self-compatible biennial Sabatia angularis (Gentianaceae): untangling the role of multiple components of pollination.
[So] Source:Ann Bot;119(1):167-176, 2017 Jan.
[Is] ISSN:1095-8290
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: Plasticity of floral traits in response to pollination can enable plants to maximize opportunities for pollen import and export under poor pollination conditions, while minimizing costs under favourable ones. Both floral longevity and display are key traits influencing pollination. While pollination-induced flower wilting is widely documented, we lack an understanding of the multifactorial complexity of this response, including the influence of other pollination components, costs of extended longevity and subsequent impacts on floral display. METHODS: Plasticity of floral longevity was experimentally evaluated in Sabatia angularis in response to multiple pollination factors: pollen addition, removal, and source (self, single-donor outcross, multiple-donor outcross) and timing of pollination. Effects of pollen quantity were further evaluated by exploiting variation in autonomous self-pollen deposition. Delayed pollination costs were tested comparing seed set from early versus late pollinations. Finally, I compared floral display metrics (peak floral display, time to peak flower, flowering duration, mean flowering rate) between experimentally pollinated and control plants. KEY RESULTS: Floral longevity was highly plastic in response to pollen addition and its timing, and the response was dose-dependent but insensitive to pollen source. Pollen removal tended to extend floral longevity, but only insofar as it precluded pollination-induced wilting via autonomous self-pollination. Under delayed pollination, the wilting response was faster and no cost was detected. Pollination further led to reduced peak floral displays and condensed flowering periods. CONCLUSIONS: Floral longevity and display plasticity could optimize fitness in S. angularis, a species prone to pollen limitation and high inbreeding depression. Under pollinator scarcity, extended floral longevities offer greater opportunities for pollen receipt and export at no cost to seed set, reproductive assurance via autonomous self-pollination and larger, more attractive floral displays. Under high pollinator availability, shortened longevities lead to smaller displays that should lower the risk of geitonogamy.
[Mh] Termos MeSH primário: Flores/fisiologia
Gentianaceae/fisiologia
Polinização/fisiologia
[Mh] Termos MeSH secundário: Cruzamentos Genéticos
Flores/anatomia & histologia
Gentianaceae/anatomia & histologia
Autoincompatibilidade em Angiospermas/fisiologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170108
[St] Status:MEDLINE
[do] DOI:10.1093/aob/mcw195


  6 / 166 MEDLINE  
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[PMID]:27941096
[Au] Autor:Voillemot M; Pannell JR
[Ad] Endereço:Department of Ecology and Evolution, Biophore Building, University of Lausanne, CH-1015 Lausanne, Switzerland marie.voillemot@unil.ch.
[Ti] Título:Maintenance of mixed mating after the loss of self-incompatibility in a long-lived perennial herb.
[So] Source:Ann Bot;119(1):177-190, 2017 Jan.
[Is] ISSN:1095-8290
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: Many hermaphroditic plants avoid self-fertilization by means of a molecular self-incompatibility (SI) system, a complex trait that is difficult to evolve but relatively easy to lose. Loss of SI is a prerequisite for an evolutionary transition from obligate outcrossing to self-fertilization, which may bring about rapid changes in the genetic diversity and structure of populations. Loss of SI is also often followed by the evolution of a 'selfing syndrome', with plants having small flowers, little nectar and few pollen grains per ovule. Here, we document the loss of SI in the long-lived Spanish toadflax Linaria cavanillesii, which has led to mixed mating rather than a transition to a high rate of selfing and in which an outcrossing syndrome has been maintained. METHODS: We performed crosses within and among six populations of L. cavanillesii in the glasshouse, measured floral traits in a common-garden experiment, performed a pollen-limitation experiment in the field and conducted population genetic analyses using microsatellites markers. KEY RESULTS: Controlled crosses revealed variation in SI from fully SI through intermediate SI to fully self-compatible (SC). Flowers of SC individuals showed no evidence of a selfing syndrome. Although the SC population of L. cavanillesii had lower within-population genetic diversity than SI populations, as expected, population differentiation among all populations was extreme and represents an F outlier in the distribution for both selfing and outcrossing species of flowering plants. CONCLUSIONS: Together, our results suggest that the transition to SC in L. cavanillesii has probably been very recent, and may have been aided by selection during or following a colonization bottleneck rather than in the absence of pollinators. We find little indication that the transition to SC has been driven by selection for reproductive assurance under conditions currently prevailing in natural populations.
[Mh] Termos MeSH primário: Linaria/fisiologia
Polinização/fisiologia
Autoincompatibilidade em Angiospermas/fisiologia
[Mh] Termos MeSH secundário: Cruzamentos Genéticos
Frutas/fisiologia
Variação Genética
Linaria/genética
Reprodução/fisiologia
Sementes/fisiologia
Espanha
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE
[do] DOI:10.1093/aob/mcw203


  7 / 166 MEDLINE  
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[PMID]:27933381
[Au] Autor:Liang M; Yang W; Su S; Fu L; Yi H; Chen C; Deng X; Chai L
[Ad] Endereço:Key Laboratory of Horticultural Plant Biology, Ministry of Education, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region), MOA, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
[Ti] Título:Genome-wide identification and functional analysis of S-RNase involved in the self-incompatibility of citrus.
[So] Source:Mol Genet Genomics;292(2):325-341, 2017 Apr.
[Is] ISSN:1617-4623
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:S-RNase-based self-incompatibility is found in Solanaceae, Rosaceae, and Scrophulariaceae, and is the most widespread mechanism that prevents self-fertilization in plants. Although 'Shatian' pummelo (Citrus grandis), a traditional cultivated variety, possesses the self-incompatible trait, the role of S-RNases in the self-incompatibility of 'Shatian' pummelo is poorly understood. To identify genes associated with self-incompatibility in citrus, we identified 16 genes encoding homologs of ribonucleases in the genomes of sweet orange (Citrus sinensis) and clementine mandarin (Citrus clementine). We preliminarily distinguished S-RNases from S-like RNases with a phylogenetic analysis that classified these homologs into three groups, which is consistent with the previous reports. Expression analysis provided evidence that CsRNS1 and CsRNS6 are S-like RNase genes. The expression level of CsRNS1 was increased during fruit development. The expression of CsRNS6 was increased during the formation of embryogenic callus. In contrast, we found that CsRNS3 possessed several common characteristics of the pistil determinant of self-incompatibility: it has an alkaline isoelectric point (pI), harbors only one intron, and is specifically expressed in style. We obtained a cDNA encoding CgRNS3 from 'Shatian' pummelo and found that it is homolog to CsRNS3 and that CgRNS3 exhibited the same expression pattern as CsRNS3. In an in vitro culture system, the CgRNS3 protein significantly inhibited the growth of self-pollen tubes from 'Shatian' pummelo, but after a heat treatment, this protein did not significantly inhibit the elongation of self- or non-self-pollen tubes. In conclusion, an S-RNase gene, CgRNS3, was obtained by searching the genomes of sweet orange and clementine for genes exhibiting sequence similarity to ribonucleases followed by expression analyses. Using this approach, we identified a protein that significantly inhibited the growth of self-pollen tubes, which is the defining property of an S-RNase.
[Mh] Termos MeSH primário: Citrus/genética
Ribonucleases/genética
Autoincompatibilidade em Angiospermas/genética
[Mh] Termos MeSH secundário: DNA Complementar/metabolismo
Flores/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Estudos de Associação Genética
Genoma de Planta
Genótipo
Família Multigênica
Filogenia
Proteínas de Plantas/genética
Pólen
Regiões Promotoras Genéticas
Proteínas Recombinantes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Plant Proteins); 0 (Recombinant Proteins); EC 3.1.- (Ribonucleases); EC 3.1.4.- (ribonuclease S)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1007/s00438-016-1279-8


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[PMID]:27870312
[Au] Autor:Duarte MO; Mendes-Rodrigues C; Alves MF; Oliveira PE; Sampaio DS
[Ad] Endereço:Instituto de Biologia, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil.
[Ti] Título:Mixed pollen load and late-acting self-incompatibility flexibility in Adenocalymma peregrinum (Miers) L.G. Lohmann (Bignonieae: Bignoniaceae).
[So] Source:Plant Biol (Stuttg);19(2):140-146, 2017 Mar.
[Is] ISSN:1438-8677
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mixed cross and self-pollen load on the stigma (mixed pollination) of species with late-acting self-incompatibility system (LSI) can lead to self-fertilized seed production. This "cryptic self-fertility" may allow selfed seedling development in species otherwise largely self-sterile. Our aims were to check if mixed pollinations would lead to fruit set in LSI Adenocalymma peregrinum, and test for evidence of early-acting inbreeding depression in putative selfed seeds from mixed pollinations. Experimental pollinations were carried out in a natural population. Fruit and seed set from self-, cross and mixed pollinations were analysed. Further germination tests were carried out for the seeds obtained from treatments. Our results confirm self-incompatibility, and fruit set from cross-pollinations was three-fold that from mixed pollinations. This low fruit set in mixed pollinations is most likely due to a greater number of self- than cross-fertilized ovules, which promotes LSI action and pistil abortion. Likewise, higher percentage of empty seeds in surviving fruits from mixed pollinations compared with cross-pollinations is probably due to ovule discounting caused by self-fertilization. Moreover, germinability of seeds with developed embryos was lower in fruits from mixed than from cross-pollinations, and the non-viable seeds from mixed pollinations showed one-third of the mass of those from cross-pollinations. The great number of empty seeds, lower germinability, lower mass of non-viable seeds, and higher variation in seed mass distribution in mixed pollinations, strongly suggests early-acing inbreeding depression in putative selfed seeds. In this sense, LSI and inbreeding depression acting together probably constrain self-fertilized seedling establishment in A. peregrinum.
[Mh] Termos MeSH primário: Bignoniaceae/fisiologia
Depressão por Endogamia
Polinização
Autoincompatibilidade em Angiospermas
[Mh] Termos MeSH secundário: Bignoniaceae/genética
Bignoniaceae/crescimento & desenvolvimento
Biomassa
Flores/genética
Flores/crescimento & desenvolvimento
Flores/fisiologia
Frutas/genética
Frutas/crescimento & desenvolvimento
Frutas/fisiologia
Germinação
Óvulo Vegetal/genética
Óvulo Vegetal/crescimento & desenvolvimento
Óvulo Vegetal/fisiologia
Pólen/genética
Pólen/crescimento & desenvolvimento
Pólen/fisiologia
Plântulas/genética
Plântulas/crescimento & desenvolvimento
Plântulas/fisiologia
Sementes/genética
Sementes/crescimento & desenvolvimento
Sementes/fisiologia
Autofertilização
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161122
[St] Status:MEDLINE
[do] DOI:10.1111/plb.12526


  9 / 166 MEDLINE  
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[PMID]:27804968
[Au] Autor:Mable BK; Hagmann J; Kim ST; Adam A; Kilbride E; Weigel D; Stift M
[Ad] Endereço:Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Glasgow, UK.
[Ti] Título:What causes mating system shifts in plants? Arabidopsis lyrata as a case study.
[So] Source:Heredity (Edinb);118(1):52-63, 2017 Jan.
[Is] ISSN:1365-2540
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The genetic breakdown of self-incompatibility (SI) and subsequent mating system shifts to inbreeding has intrigued evolutionary geneticists for decades. Most of our knowledge is derived from interspecific comparisons between inbreeding species and their outcrossing relatives, where inferences may be confounded by secondary mutations that arose after the initial loss of SI. Here, we study an intraspecific breakdown of SI and its consequences in North American Arabidopsis lyrata to test whether: (1) particular S-locus haplotypes are associated with the loss of SI and/or the shift to inbreeding; (2) a population bottleneck may have played a role in driving the transition to inbreeding; and (3) the mutation(s) underlying the loss of SI are likely to have occurred at the S-locus. Combining multiple approaches for genotyping, we found that outcrossing populations on average harbour 5 to 9 S-locus receptor kinase (SRK) alleles, but only two, S1 and S19, are shared by most inbreeding populations. Self-compatibility (SC) behaved genetically as a recessive trait, as expected from a loss-of-function mutation. Bulked segregant analysis in SC × SI F2 individuals using deep sequencing confirmed that all SC plants were S1 homozygotes but not all S1 homozygotes were SC. This was also revealed in population surveys, where only a few S1 homozygotes were SC. Together with crossing data, this suggests that there is a recessive factor that causes SC that is physically unlinked to the S-locus. Overall, our results emphasise the value of combining classical genetics with advanced sequencing approaches to resolve long outstanding questions in evolutionary biology.
[Mh] Termos MeSH primário: Arabidopsis/genética
Endogamia
Proteínas de Plantas/genética
Proteínas Quinases/genética
Autoincompatibilidade em Angiospermas/genética
[Mh] Termos MeSH secundário: Arabidopsis/fisiologia
Evolução Biológica
Genes de Plantas
Genética Populacional
Great Lakes Region
Haplótipos
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); EC 2.7.- (Protein Kinases); EC 2.7.11.1 (S-receptor kinase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161103
[St] Status:MEDLINE
[do] DOI:10.1038/hdy.2016.99


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[PMID]:27864264
[Au] Autor:Melen MK; Herman JA; Lucas J; O'Malley RE; Parker IM; Thom AM; Whittall JB
[Ad] Endereço:Department of Environmental Studies, San Jose State University, One Washington Square, San Jose, California 95192 USA.
[Ti] Título:Reproductive success through high pollinator visitation rates despite self incompatibility in an endangered wallflower.
[So] Source:Am J Bot;103(11):1979-1989, 2016 Nov.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Self incompatibility (SI) in rare plants presents a unique challenge-SI protects plants from inbreeding depression, but requires a sufficient number of mates and xenogamous pollination. Does SI persist in an endangered polyploid? Is pollinator visitation sufficient to ensure reproductive success? Is there evidence of inbreeding/outbreeding depression? We characterized the mating system, primary pollinators, pollen limitation, and inbreeding/outbreeding depression in Erysimum teretifolium to guide conservation efforts. METHODS: We compared seed production following self pollination and within- and between-population crosses. Pollen tubes were visualized after self pollinations and between-population pollinations. Pollen limitation was tested in the field. Pollinator observations were quantified using digital video. Inbreeding/outbreeding depression was assessed in progeny from self and outcross pollinations at early and later developmental stages. KEY RESULTS: Self-pollination reduced seed set by 6.5× and quadrupled reproductive failure compared with outcross pollination. Pollen tubes of some self pollinations were arrested at the stigmatic surface. Seed-set data indicated strong SI, and fruit-set data suggested partial SI. Pollinator diversity and visitation rates were high, and there was no evidence of pollen limitation. Inbreeding depression (δ) was weak for early developmental stages and strong for later developmental stages, with no evidence of outbreeding depression. CONCLUSIONS: The rare hexaploid E. teretifolium is largely self incompatible and suffers from late-acting inbreeding depression. Reproductive success in natural populations was accomplished through high pollinator visitation rates consistent with a lack of pollen limitation. Future reproductive health for this species will require large population sizes with sufficient mates and a robust pollinator community.
[Mh] Termos MeSH primário: Erysimum/fisiologia
Insetos/fisiologia
Polinização
[Mh] Termos MeSH secundário: Animais
Erysimum/genética
Erysimum/crescimento & desenvolvimento
Flores/genética
Flores/crescimento & desenvolvimento
Flores/fisiologia
Frutas/genética
Frutas/crescimento & desenvolvimento
Frutas/fisiologia
Depressão por Endogamia
Pólen/genética
Pólen/crescimento & desenvolvimento
Pólen/fisiologia
Tubo Polínico/genética
Tubo Polínico/crescimento & desenvolvimento
Tubo Polínico/fisiologia
Poliploidia
Reprodução
Sementes/genética
Sementes/crescimento & desenvolvimento
Sementes/fisiologia
Autofertilização
Autoincompatibilidade em Angiospermas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161120
[St] Status:MEDLINE



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