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Pesquisa : H01.158.201 [Categoria DeCS]
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  1 / 10451 MEDLINE  
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[PMID]:29236394
[Au] Autor:Vynogradova RP; Danilova VM; Yurasova SP
[Ti] Título:[Scientific and practical activity of the Department of Muscle Biochemistry of the Palladin Institute of Biochemistry of NAS of Ukraine].
[So] Source:Ukr Biochem J;89(1):90-101, 2017 Jan-Dec.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:rus; ukr
[Ab] Resumo:The article focuses on scientific and practical activity of the Department of Muscle Biochemistry of the Palladin Institute of Biochemistry of NAS of Ukraine in the context of its foundation and development. Main findings and practical achievements in the area of muscle biochemistry are summarized and discussed.
[Mh] Termos MeSH primário: Academias e Institutos/história
Bioquímica/história
Músculos/metabolismo
[Mh] Termos MeSH secundário: Bioquímica/recursos humanos
História do Século XX
História do Século XXI
Seres Humanos
Ucrânia
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj89.01.090


  2 / 10451 MEDLINE  
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[PMID]:29236382
[Au] Autor:Danilova VM; Vynogradova RP; Chernysh IG; Petrenko TM
[Ti] Título:[Inventive activity of the Department of Metabolism Regulation of the Palladin Institute of Biochemistry of NAS of Ukraine].
[So] Source:Ukr Biochem J;88(6):110-21, 2016 Nov-Dec.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:rus; ukr
[Ab] Resumo:The article is devoted to the inventive activity of the Department of Metabolism Regulation of the Palladin Institute of Biochemistry of NAS of Ukraine in the context of the history of its inception, development and the research activities of its founder, academician of NAS of Ukraine M. F. Guly as well as his students and followers. It briefly tells about practical achievements of M. F. Guly which were as significant, immense and diverse as his scientific accomplishments. The paper analyses in detail the practical results of scientific research of his students and followers aimed to solve practical problems of medicine, food-processing, agriculture, and which are essentially a continuation of the ideas and projects of M. F. Guly.
[Mh] Termos MeSH primário: Academias e Institutos/história
Bioquímica/história
Invenções/história
[Mh] Termos MeSH secundário: Bioquímica/recursos humanos
História do Século XX
História do Século XXI
Ucrânia
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.06.110


  3 / 10451 MEDLINE  
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[PMID]:29235825
[Au] Autor:Danilova VM; Vynogradova RP; Torkhova SG
[Ti] Título:[Inventive activity of the Department of Molecular Immunology of the Palladin Institute of Biochemistry of NAS of Ukraine].
[So] Source:Ukr Biochem J;88(5):116-35, 2016 Sep-Oct.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:rus; ukr
[Ab] Resumo:The article is devoted to the inventive activity of the Department of Molecular Immunology of the Palladin Institute of Biochemistry of NAS of Ukraine in the context of the history of its inception, development and in the context of scholarly and organizational activities of Sergii Vasyl'ovych Komisarenko. This autumn marks 50th anniversary since young Sergii Komisarenko (now ­ Academician of NAS and NAMS of Ukraine, Dr. Biol. Sci., Professor) has joined the Palladin Institute of Biochemistry, has completed all stages of the academic carrier from PhD student to Head of the Institute. He is the first in Ukraine who started the new branch of research ­ molecular immunology, created a strong scientific school, which earned worldwide acclaim and made significant contribution to finding solutions to current problems in human health sciences. S.V. Komisarenko was among those, who were first in the USSR to use immunoenzyme and flow cytofluometric assays, hybridoma technology for producing monoclonal antibodies and immunochemical assay of proteins, which became the basis for development of highly sensitive and highly specific immunodiagnostic systems, which are of high necessity in medicine, veterinary, development of immunotechnologies, environment monitoring, etc. Under his leadership the Department has made a series of important discoveries and developments including relating to antitumour immunotoxins, effects of low dose radiation on the immune system of Chernobyl liquidators, immunochemical structure of neurotoxin apamine, cytochrom c, fibrinogen and fibrin molecules at different stages of polymerization, diphtheria toxin and its receptor, tuberculosis causing micobacterium, roles of protease-activated receptors (PARs) and nicotinic acetylcholine receptors of lymphocytes, nature of polyreactive immunoglobulins (PRIGs), among other important scientific contributions. S.V. Komisarenko and his colleagues also hold numerous (more than 80) author's certificates and patents in Ukraine and USSR.
[Mh] Termos MeSH primário: Academias e Institutos/história
Alergia e Imunologia/história
Bioquímica/história
Invenções/história
[Mh] Termos MeSH secundário: Alergia e Imunologia/recursos humanos
Bioquímica/recursos humanos
História do Século XX
História do Século XXI
Seres Humanos
Imunoglobulinas/análise
Ucrânia
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Nm] Nome de substância:
0 (Immunoglobulins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.05.116


  4 / 10451 MEDLINE  
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[PMID]:29235792
[Au] Autor:Danilova VM; Vynogradova RP; Chernysh IY
[Ti] Título:[Inventive activity of the Departments of Chemistry and Biochemistry of Enzymes, and Protein Structure and Function of the Palladin Institute of Biochemistry of NAS of Ukraine. Part III. Diagnostic test-systems for analysis of fibrinolysis blood system and novel approaches to thrombosis treatment].
[So] Source:Ukr Biochem J;88(4):129-37, 2016 Jul-Aug.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:rus; ukr
[Ab] Resumo:This article continues analysis of scientific achievements of the Institute of Biochemistry in the study of hemostasis system. Two previous articles were focused on the studies of blood coagulation proteins and development of the immune-enzyme test-systems for evaluation of the risk of thrombosis upon various pathologies. This article highlights the research on the blood fibrinolysis system and new approaches to thrombosis treatment, which were developed (and are under development) in the Palladin Institute of Biochemistry of the NAS of Ukraine, in particular, in the Department of Chemistry and Biochemistry of Enzymes headed previously by Dr.Sci.(Biol.) S. O. Kudinov and now by Dr.Sci.(Biol.) T .V. Grinenko, and also in the Department of Protein Structure and Function headed by Dr.Biol.Sci. E. M. Makogonenko. The fundamental knowledge of protein molecule functions and mechanisms of regulation of blood coagulation and fibrinolysis opens up new opportunities to diagnose hemostasis disorders and control the effectiveness of the cardiovascular disease treatment and also contributes to development of new techniques for isolation of new proteins ­ promising therapeutic agents.
[Mh] Termos MeSH primário: Academias e Institutos/história
Bioquímica/história
Transtornos da Coagulação Sanguínea/diagnóstico
Testes Diagnósticos de Rotina/história
Invenções/história
[Mh] Termos MeSH secundário: Bioquímica/recursos humanos
Transtornos da Coagulação Sanguínea/história
Fibrinólise/fisiologia
História do Século XX
História do Século XXI
Seres Humanos
Ucrânia
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.04.129


  5 / 10451 MEDLINE  
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[PMID]:29235336
[Au] Autor:Lugovska NE
[Ti] Título:[Inventive activity of the Departments of Protein Structure and Function, and Molecular Immunology of the Palladin Institute of Biochemistry of NAS of Ukraine. Part II. National breakthrough in the study and diagnostics of human hemostasis system].
[So] Source:Ukr Biochem J;88(3):106-18, 2016 May-Jun.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:rus; ukr
[Ab] Resumo:The scientists of Protein Structure and Function, and Molecular Immunology Departments of the Palladin Institute of Biochemistry (NAS of Ukraine) under the supervision of member of NASU and NAMSU, prof. S. V. Komisarenko and corresponding member of NASU prof. E. V. Lugovskoy have made the real breakthrough in the field of research of the mechanisms of fibrin polymerization and formation of fibrin framework of thrombi. The immunodiagnostic test-systems for the evaluation of the risk of thrombus formation were developed for the first time. Researches have obtained the monoclonal antibodies to fibrinogen, fibrin, D-dimer and their fragments. These monoclonal antibodies were used as molecular probes for the localization of newly detected fibrin polymerization sites. Obtained antibodies with high affinity interact with fibrinogen, D-dimer and soluble fibrin ­ main markers of the risk of thrombus formation. They were used for the development of the immunodiagnostic test-systems to quantify these markers in human blood plasma for the evaluation of the state of haemostasis system, detection of prethrombotic states, disseminated intravascular coagulation, detection of thrombosis and monitoring of antithrombotic and fibrinolytic therapy. The successful trial of developed test-systems was carried out in clinics of Ukraine, and the State registration was obtained for the implementation of them into the clinical practice. Presented works were awarded State prize of Ukraine in Science and technology.
[Mh] Termos MeSH primário: Academias e Institutos/história
Alergia e Imunologia/história
Bioquímica/história
Transtornos da Coagulação Sanguínea/diagnóstico
Invenções/história
[Mh] Termos MeSH secundário: Alergia e Imunologia/recursos humanos
Bioquímica/recursos humanos
Transtornos da Coagulação Sanguínea/história
História do Século XX
História do Século XXI
Seres Humanos
Ucrânia
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.03.106


  6 / 10451 MEDLINE  
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[PMID]:29227613
[Au] Autor:Danilova LD; Vynogradova RP; Grigorieva MV
[Ti] Título:[Inventive activity of the Department of Protein Structure and Function of the Palladin Institute of Biochemistry of NAS of Ukraine. Part I. Development of the diagnostic methods for detection of hemostasis disorders and characterization of certain blood coagulation factors].
[So] Source:Ukr Biochem J;88(2):107-18, 2016 Mar-Apr.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:rus; ukr
[Mh] Termos MeSH primário: Academias e Institutos/história
Bioquímica/história
Transtornos Herdados da Coagulação Sanguínea/diagnóstico
Invenções/história
[Mh] Termos MeSH secundário: Bioquímica/recursos humanos
Transtornos Herdados da Coagulação Sanguínea/história
Fatores de Coagulação Sanguínea/história
Fatores de Coagulação Sanguínea/metabolismo
Fatores de Coagulação Sanguínea/ultraestrutura
História do Século XX
História do Século XXI
Seres Humanos
Ucrânia
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Nm] Nome de substância:
0 (Blood Coagulation Factors)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.02.107


  7 / 10451 MEDLINE  
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[PMID]:29227595
[Au] Autor:Vynogradova RP; Lugovska GG; Yurasova SP
[Ti] Título:[Analysis of inventive activity of the department of vitamin and coenzyme biochemistry of Palladin Institute of Biochemistry NAS of Ukraine in the period of 1980-2015].
[So] Source:Ukr Biochem J;88(1):126-40, 2016 Jan-Feb.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:rus; ukr
[Mh] Termos MeSH primário: Academias e Institutos/história
Bioquímica/história
Invenções/história
[Mh] Termos MeSH secundário: Coenzimas/metabolismo
Coenzimas/farmacologia
História do Século XX
História do Século XXI
Ucrânia
Vitaminas/metabolismo
Vitaminas/farmacologia
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Nm] Nome de substância:
0 (Coenzymes); 0 (Vitamins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.01.126


  8 / 10451 MEDLINE  
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[PMID]:29251437
[Au] Autor:Chenette EJ; Martin SJ
[Ad] Endereço:The FEBS Journal Editorial Office, Cambridge, UK.
[Ti] Título:50 years of The FEBS Journal: looking back as well as ahead.
[So] Source:FEBS J;284(24):4162-4171, 2017 Dec.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this last issue of 2017, we're celebrating the 50th anniversary of The FEBS Journal. This Editorial considers how the journal has grown and changed from volume 1, issue 1 and outlines our exciting plans for the future.
[Mh] Termos MeSH primário: Publicações Periódicas como Assunto/história
[Mh] Termos MeSH secundário: Bibliometria
Bioquímica/história
Europa (Continente)
Alemanha
História do Século XX
História do Século XXI
Publicações Periódicas como Assunto/tendências
Sociedades Científicas/história
[Pt] Tipo de publicação:EDITORIAL; HISTORICAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1111/febs.14328


  9 / 10451 MEDLINE  
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[PMID]:28747322
[Au] Autor:Panchapakesan SSS; Ferguson ML; Hayden EJ; Chen X; Hoskins AA; Unrau PJ
[Ad] Endereço:Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
[Ti] Título:Ribonucleoprotein purification and characterization using RNA Mango.
[So] Source:RNA;23(10):1592-1599, 2017 10.
[Is] ISSN:1469-9001
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The characterization of RNA-protein complexes (RNPs) is a difficult but increasingly important problem in modern biology. By combining the compact RNA Mango aptamer with a fluorogenic thiazole orange desthiobiotin (TO1-Dtb or TO3-Dtb) ligand, we have created an RNA tagging system that simplifies the purification and subsequent characterization of endogenous RNPs. Mango-tagged RNP complexes can be immobilized on a streptavidin solid support and recovered in their native state by the addition of free biotin. Furthermore, Mango-based RNP purification can be adapted to different scales of RNP isolation ranging from pull-down assays to the isolation of large amounts of biochemically defined cellular RNPs. We have incorporated the Mango aptamer into the U1 small nuclear RNA (snRNA), shown that the Mango-snRNA is functional in cells, and used the aptamer to pull down a U1 snRNA-associated protein. To demonstrate large-scale isolation of RNPs, we purified and characterized bacterial RNA polymerase holoenzyme (HE) in complex with a Mango-containing 6S RNA. We were able to use the combination of a red-shifted TO3-Dtb ligand and eGFP-tagged HE to follow the binding and release of the 6S RNA by two-color native gel analysis as well as by single-molecule fluorescence cross-correlation spectroscopy. Together these experiments demonstrate how the Mango aptamer in conjunction with simple derivatives of its flurophore ligands enables the purification and characterization of endogenous cellular RNPs in vitro.
[Mh] Termos MeSH primário: Aptâmeros de Nucleotídeos/química
Bioquímica/métodos
Ribonucleoproteínas/isolamento & purificação
Espectrometria de Fluorescência/métodos
[Mh] Termos MeSH secundário: Benzotiazóis/química
Biotina/análogos & derivados
Biotina/química
Proteínas de Fluorescência Verde/genética
Quinolinas/química
RNA Bacteriano/metabolismo
RNA Nuclear Pequeno/química
RNA não Traduzido/metabolismo
Ribonucleoproteínas/metabolismo
Saccharomyces cerevisiae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (6S RNA); 0 (Aptamers, Nucleotide); 0 (Benzothiazoles); 0 (Quinolines); 0 (RNA, Bacterial); 0 (RNA, Small Nuclear); 0 (RNA, Untranslated); 0 (Ribonucleoproteins); 0 (U1 small nuclear RNA); 107091-89-4 (thiazole orange); 147336-22-9 (Green Fluorescent Proteins); 6SO6U10H04 (Biotin); 71U5JB52KS (desthiobiotin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1261/rna.062166.117


  10 / 10451 MEDLINE  
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[PMID]:28934475
[Au] Autor:Incarnato D; Morandi E; Anselmi F; Simon LM; Basile G; Oliviero S
[Ad] Endereço:Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Via Accademia Albertina, 13, Torino, Italy.
[Ti] Título:In vivo probing of nascent RNA structures reveals principles of cotranscriptional folding.
[So] Source:Nucleic Acids Res;45(16):9716-9725, 2017 Sep 19.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Defining the in vivo folding pathway of cellular RNAs is essential to understand how they reach their final native conformation. We here introduce a novel method, named Structural Probing of Elongating Transcripts (SPET-seq), that permits single-base resolution analysis of transcription intermediates' secondary structures on a transcriptome-wide scale, enabling base-resolution analysis of the RNA folding events. Our results suggest that cotranscriptional RNA folding in vivo is a mixture of cooperative folding events, in which local RNA secondary structure elements are formed as they get transcribed, and non-cooperative events, in which 5'-halves of long-range helices get sequestered into transient non-native interactions until their 3' counterparts have been transcribed. Together our work provides the first transcriptome-scale overview of RNA cotranscriptional folding in a living organism.
[Mh] Termos MeSH primário: Dobramento de RNA
RNA/química
[Mh] Termos MeSH secundário: Bioquímica/métodos
Escherichia coli/genética
Conformação de Ácido Nucleico
Ribonuclease P/química
Ribonuclease P/metabolismo
Ésteres do Ácido Sulfúrico/química
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sulfuric Acid Esters); 63231-63-0 (RNA); EC 3.1.26.5 (Ribonuclease P); JW5CW40Z50 (dimethyl sulfate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx617



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