[PMID]: | 21519905 |
[Au] Autor: | Yang JL; Mu H; Lü ZR; Yin SJ; Si YX; Zhou SM; Zhang F; Hu WJ; Meng FG; Zhou HM; Zhang ZP; Qian GY |
[Ad] Endereço: | School of Life Science, Ningxia University, Yinchuan 750021, People's Republic of China. |
[Ti] Título: | Trehalose has a protective effect on human brain-type creatine kinase during thermal denaturation. |
[So] Source: | Appl Biochem Biotechnol;165(2):476-84, 2011 Sep. |
[Is] ISSN: | 1559-0291 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | We investigated the effects of trehalose on thermal inactivation and aggregation of human brain-type creatine kinase (hBBCK) in this study. In the presence of 1.0 M trehalose, the midpoint temperature of thermal inactivation (T (m)) of hBBCK increased by 4.6 °C, and the activation energy (E (a)) for thermal inactivation increased from 29.7 to 41.1 kJ mol(-1). Intrinsic fluorescence spectra also showed an increase in the apparent transition temperature (T (1/2)) of hBBCK from 43.0 °C to 46.5 °C, 47.7 °C, and 49.9 °C in 0, 0.6, 0.8, and 1.2 M trehalose, respectively. In addition, trehalose significantly blocked the aggregation of hBBCK during thermal denaturation. Our results indicate that trehalose has potential applications as a thermal stabilizer and may aid in the folding of other enzymes in addition to hBBCK. |
[Mh] Termos MeSH primário: |
Bioquímica de Carboidratos/métodos Creatina Quinase Forma BB/metabolismo Desnaturação Proteica/efeitos dos fármacos Trealose/farmacologia
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[Mh] Termos MeSH secundário: |
Creatina Quinase Forma BB/química Estabilidade Enzimática/efeitos dos fármacos Temperatura Alta Seres Humanos Cinética Dobramento de Proteína/efeitos dos fármacos Espectrometria de Fluorescência Termodinâmica Trealose/química
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[Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| B8WCK70T7I (Trehalose); EC 2.7.3.2 (Creatine Kinase, BB Form) |
[Em] Mês de entrada: | 1201 |
[Cu] Atualização por classe: | 131121 |
[Lr] Data última revisão:
| 131121 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 110427 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1007/s12010-011-9266-3 |
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