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Pesquisa : H01.158.273.248.750.249 [Categoria DeCS]
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[PMID]:29268103
[Au] Autor:Carusso S; Juárez AB; Moretton J; Magdaleno A
[Ad] Endereço:Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Salud Pública e Higiene Ambiental, Junin 956, 4° Piso, C1113 AAC, Buenos Aires, Argentina; Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental y Departa
[Ti] Título:Effects of three veterinary antibiotics and their binary mixtures on two green alga species.
[So] Source:Chemosphere;194:821-827, 2018 Mar.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The individual and combined toxicities of chlortetracycline (CTC), oxytetracycline (OTC) and enrofloxacin (ENF) have been examined in two green algae representative of the freshwater environment, the international standard strain Pseudokichneriella subcapitata and the native strain Ankistrodesmus fusiformis. The toxicities of the three antibiotics and their mixtures were similar in both strains, although low concentrations of ENF and CTC + ENF were more toxic in A. fusiformis than in the standard strain. The toxicological interactions of binary mixtures were predicted using the two classical models of additivity: Concentration Addition (CA) and Independent Action (IA), and compared to the experimentally determined toxicities over a range of concentrations between 0.1 and 10 mg L . The CA model predicted the inhibition of algal growth in the three mixtures in P. subcapitata, and in the CTC + OTC and CTC + ENF mixtures in A. fusiformis. However, this model underestimated the experimental results obtained in the OTC + ENF mixture in A. fusiformis. The IA model did not predict the experimental toxicological effects of the three mixtures in either strain. The sum of the toxic units (TU) for the mixtures was calculated. According to these values, the binary mixtures CTC + ENF and OTC + ENF showed an additive effect, and the CTC + OTC mixture showed antagonism in P. subcapitata, whereas the three mixtures showed synergistic effects in A. fusiformis. Although A. fusiformis was isolated from a polluted river, it showed a similar sensitivity with respect to P. subcapitata when it was exposed to binary mixtures of antibiotics.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Clorófitas/efeitos dos fármacos
Drogas Veterinárias/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/toxicidade
Clortetraciclina/toxicidade
Interações Medicamentosas
Fluoroquinolonas/toxicidade
Biologia de Ecossistemas de Água Doce
Oxitetraciclina/toxicidade
Drogas Veterinárias/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Fluoroquinolones); 0 (Veterinary Drugs); 3DX3XEK1BN (enrofloxacin); WCK1KIQ23Q (Chlortetracycline); X20I9EN955 (Oxytetracycline)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE


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[PMID]:28336620
[Au] Autor:Enserink M
[Ti] Título:Fishy business.
[So] Source:Science;355(6331):1254-1257, 2017 Mar 24.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Biologia de Ecossistemas de Água Doce
Biologia Marinha
Percas
Má Conduta Científica
[Mh] Termos MeSH secundário: Animais
Ingestão de Alimentos
Esocidae
Cadeia Alimentar
Óvulo
Publicações Periódicas como Assunto
Plásticos
Suécia
Poluentes da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plastics); 0 (Water Pollutants)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1126/science.355.6331.1254


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[PMID]:27500403
[Au] Autor:Clewing C; Albrecht C; Wilke T
[Ad] Endereço:Department of Animal Ecology and Systematics, Justus Liebig University Giessen, Giessen, Germany.
[Ti] Título:A Complex System of Glacial Sub-Refugia Drives Endemic Freshwater Biodiversity on the Tibetan Plateau.
[So] Source:PLoS One;11(8):e0160286, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although only relatively few freshwater invertebrate families are reported from the Tibetan Plateau, the degree of endemism may be high. Many endemic lineages occur within permafrost areas, raising questions about the existence of isolated intra-plateau glacial refugia. Moreover, if such refugia existed, it might be instructive to learn whether they were associated with lakes or with more dynamic ecosystems such as ponds, wetlands, or springs. To study these hypotheses, we used pulmonate snails of the plateau-wide distributed genus Radix as model group and the Lake Donggi Cona drainage system, located in the north-eastern part of the plateau, as model site. First, we performed plateau-wide phylogenetic analyses using mtDNA data to assess the overall relationships of Radix populations inhabiting the Lake Donggi Cona system for revealing refugial lineages. We then conducted regional phylogeographical analyses applying a combination of mtDNA and nuclear AFLP markers to infer the local structure and demographic history of the most abundant endemic Radix clade for identifying location and type of (sub-)refugia within the drainage system. Our phylogenetic analysis showed a high diversity of Radix lineages in the Lake Donggi Cona system. Subsequent phylogeographical analyses of the most abundant endemic clade indicated a habitat-related clustering of genotypes and several Late Pleistocene spatial/demographic expansion events. The most parsimonious explanation for these patterns would be a scenario of an intra-plateau glacial refugium in the Lake Donggi Cona drainage system, which might have consisted of isolated sub-refugia. Though the underlying processes remain unknown, an initial separation of lake and watershed populations could have been triggered by lake-level fluctuations before and during the Last Glacial Maximum. This study inferred the first intra-plateau refugium for freshwater animals on the Tibetan Plateau. It thus sheds new light on the evolutionary history of its endemic taxa and provides important insights into the complex refugial history of a high-altitude ecosystem.
[Mh] Termos MeSH primário: Biodiversidade
DNA Mitocondrial/genética
Biologia de Ecossistemas de Água Doce
Caramujos/genética
[Mh] Termos MeSH secundário: Análise do Polimorfismo de Comprimento de Fragmentos Amplificados/métodos
Animais
Teorema de Bayes
Ecossistema
Evolução Molecular
Genética Populacional
Filogeografia
Dinâmica Populacional
Análise de Sequência de DNA/métodos
Tibet
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160809
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0160286


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[PMID]:27359116
[Au] Autor:Civade R; Dejean T; Valentini A; Roset N; Raymond JC; Bonin A; Taberlet P; Pont D
[Ad] Endereço:Hydrosystems and Bioprocesses Research unit, IRSTEA, Antony, France.
[Ti] Título:Spatial Representativeness of Environmental DNA Metabarcoding Signal for Fish Biodiversity Assessment in a Natural Freshwater System.
[So] Source:PLoS One;11(6):e0157366, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the last few years, the study of environmental DNA (eDNA) has drawn attention for many reasons, including its advantages for monitoring and conservation purposes. So far, in aquatic environments, most of eDNA research has focused on the detection of single species using species-specific markers. Recently, species inventories based on the analysis of a single generalist marker targeting a larger taxonomic group (eDNA metabarcoding) have proven useful for bony fish and amphibian biodiversity surveys. This approach involves in situ filtering of large volumes of water followed by amplification and sequencing of a short discriminative fragment from the 12S rDNA mitochondrial gene. In this study, we went one step further by investigating the spatial representativeness (i.e. ecological reliability and signal variability in space) of eDNA metabarcoding for large-scale fish biodiversity assessment in a freshwater system including lentic and lotic environments. We tested the ability of this approach to characterize large-scale organization of fish communities along a longitudinal gradient, from a lake to the outflowing river. First, our results confirm that eDNA metabarcoding is more efficient than a single traditional sampling campaign to detect species presence, especially in rivers. Second, the species list obtained using this approach is comparable to the one obtained when cumulating all traditional sampling sessions since 1995 and 1988 for the lake and the river, respectively. In conclusion, eDNA metabarcoding gives a faithful description of local fish biodiversity in the study system, more specifically within a range of a few kilometers along the river in our study conditions, i.e. longer than a traditional fish sampling site.
[Mh] Termos MeSH primário: Biodiversidade
Código de Barras de DNA Taxonômico
Monitoramento Ambiental/métodos
Peixes/genética
[Mh] Termos MeSH secundário: Animais
Ecossistema
Biologia de Ecossistemas de Água Doce
Lagos
Reprodutibilidade dos Testes
Rios
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160701
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0157366


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[PMID]:27352247
[Au] Autor:Salinero MC; Michalski F
[Ad] Endereço:Laboratório de Ecologia e Conservação de Vertebrados, Universidade Federal do Amapá, Macapá, Amapá, Brazil.
[Ti] Título:Implications of Scientific Collaboration Networks on Studies of Aquatic Vertebrates in the Brazilian Amazon.
[So] Source:PLoS One;11(6):e0158413, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The quantity of wildlife extracted from the Amazon has increased in the past decades as a consequence of an increase in human population density and income growth. To evaluate the spatial distribution of studies on subsistence and/or commercial hunting conducted in the Brazilian Amazon, we selected eight mid-sized and large-bodied aquatic vertebrate species with a history of human exploitation in the region. We used a combination of searches in the gray and scientific literature from the past 24 years to provide an updated distributional map of studies on the target species. We calculated the distances between the study sites and the locations of the research institutes/universities that the first and last authors of the same study were affiliated to. For the period of 1990 to 2014, we found 105 studies on the subsistence and/or commercial hunting of aquatic vertebrates in the Brazilian Amazon in 271 locations that involved 43 institutions (37 Brazilian and 6 international). The spatial distribution of the studies across the Brazilian Amazon varied, but over 80% took place in the northeast and central Amazon, encompassing three States of the Legal Brazilian Amazon (Amazonas, 51.42%; Pará, 19.05%; and Amapá, 16.19%). Over half of the research study sites (52.91%) were within 500 km of the research institute/university of the first or last authors. Some research institutes/universities did not have any inter-institutional collaborations, while others collaborated with eight or more institutes. Some research institutes/universities conducted many studies, had an extensive collaboration network, and contributed greatly to the network of studies on Amazonian aquatic vertebrates. Our research contributes to the knowledge of studies on the subsistence and/or commercial hunting of the most exploited aquatic vertebrates of the Brazilian Amazon, illustrates the impact that collaboration networks have on research, and highlights potential areas for improvement and the generation of new collaborations.
[Mh] Termos MeSH primário: Distribuição Animal
Organismos Aquáticos/fisiologia
Biologia de Ecossistemas de Água Doce/estatística & dados numéricos
[Mh] Termos MeSH secundário: Jacarés e Crocodilos/fisiologia
Animais
Brasil
Comportamento Cooperativo
Golfinhos/fisiologia
Ecossistema
Biologia de Ecossistemas de Água Doce/organização & administração
Publicações Periódicas como Assunto/estatística & dados numéricos
Rios
Trichechus/fisiologia
Tartarugas/fisiologia
Universidades/estatística & dados numéricos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160629
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0158413


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[PMID]:26928527
[Au] Autor:Katouzian AR; Sari A; Macher JN; Weiss M; Saboori A; Leese F; Weigand AM
[Ad] Endereço:School of Biology and Centre of Excellence in Phylogeny of Living Organisms, University of Tehran, Tehran, Iran.
[Ti] Título:Drastic underestimation of amphipod biodiversity in the endangered Irano-Anatolian and Caucasus biodiversity hotspots.
[So] Source:Sci Rep;6:22507, 2016 Mar 01.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Biodiversity hotspots are centers of biological diversity and particularly threatened by anthropogenic activities. Their true magnitude of species diversity and endemism, however, is still largely unknown as species diversity is traditionally assessed using morphological descriptions only, thereby ignoring cryptic species. This directly limits evidence-based monitoring and management strategies. Here we used molecular species delimitation methods to quantify cryptic diversity of the montane amphipods in the Irano-Anatolian and Caucasus biodiversity hotspots. Amphipods are ecosystem engineers in rivers and lakes. Species diversity was assessed by analysing two genetic markers (mitochondrial COI and nuclear 28S rDNA), compared with morphological assignments. Our results unambiguously demonstrate that species diversity and endemism is dramatically underestimated, with 42 genetically identified freshwater species in only five reported morphospecies. Over 90% of the newly recovered species cluster inside Gammarus komareki and G. lacustris; 69% of the recovered species comprise narrow range endemics. Amphipod biodiversity is drastically underestimated for the studied regions. Thus, the risk of biodiversity loss is significantly greater than currently inferred as most endangered species remain unrecognized and/or are only found locally. Integrative application of genetic assessments in monitoring programs will help to understand the true magnitude of biodiversity and accurately evaluate its threat status.
[Mh] Termos MeSH primário: Anfípodes/genética
Biodiversidade
DNA Mitocondrial/genética
Monitorização de Parâmetros Ecológicos
Complexo IV da Cadeia de Transporte de Elétrons/genética
RNA Ribossômico 28S/genética
[Mh] Termos MeSH secundário: Animais
Ásia Ocidental
Biologia de Ecossistemas de Água Doce
Marcadores Genéticos/genética
Variação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (Genetic Markers); 0 (RNA, Ribosomal, 28S); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE
[do] DOI:10.1038/srep22507


  7 / 58 MEDLINE  
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[PMID]:26142058
[Au] Autor:Gomes LC; Pessali TC; Sales NG; Pompeu PS; Carvalho DC
[Ad] Endereço:Programa de Pós-graduação em Zoologia de Vertebrados, Laboratório de Genética da Conservação, Pontifícia Universidade Católica de Minas Gerais, Rua Dom José Gaspar, 500,Coração Eucarístico, Belo Horizonte, MG, 30535-901, Brazil.
[Ti] Título:Integrative taxonomy detects cryptic and overlooked fish species in a neotropical river basin.
[So] Source:Genetica;143(5):581-8, 2015 Oct.
[Is] ISSN:1573-6857
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The great freshwater fish diversity found in the neotropical region makes management and conservation actions challenging. Due to shortage of taxonomists and insufficient infrastructure to deal with such great biodiversity (i.e. taxonomic impediment), proposed remedies to accelerate species identification and descriptions include techniques that combine DNA-based identification and concise morphological description. The building of a DNA barcode reference database correlating meristic and genetic data was developed for 75 % of the Mucuri River basin's freshwater fish. We obtained a total of 141 DNA barcode sequences from 37 species belonging to 30 genera, 19 families, and 5 orders. Genetic distances within species, genera, and families were 0.74, 9.5, and 18.86 %, respectively. All species could be clearly identified by the DNA barcodes. Divergences between meristic morphological characteristics and DNA barcodes revealed two cryptic species among the Cyphocharax gilbert and Astyanax gr. bimaculatus specimens, and helped to identify two overlooked species within the Gymnotus and Astyanax taxa. Therefore, using a simplified model of neotropical biodiversity, we tested the efficiency of an integrative taxonomy approach for species discovery, identification of cryptic diversity, and accelerating biodiversity descriptions.
[Mh] Termos MeSH primário: Código de Barras de DNA Taxonômico/métodos
Peixes/genética
[Mh] Termos MeSH secundário: Animais
Biodiversidade
Brasil
Characidae/genética
Classificação
Biologia de Ecossistemas de Água Doce
Filogenia
Rios
Análise de Sequência de DNA
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1606
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150705
[St] Status:MEDLINE
[do] DOI:10.1007/s10709-015-9856-z


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[PMID]:25994809
[Au] Autor:Heinrich JM; Irazoqui HA
[Ad] Endereço:Group of Innovation on Bio-processes Engineering, Institute for the Technological Development of the Chemical Industry (INTEC), National Council of Scientific and Technological Research (CONICET) and University of Litoral (UNL), Santa Fe, Argentina.
[Ti] Título:Kinetic Model of Photoautotrophic Growth of Chlorella sp. Microalga, Isolated from the Setúbal Lagoon.
[So] Source:Photochem Photobiol;91(5):1095-102, 2015 Sep-Oct.
[Is] ISSN:1751-1097
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this work, a kinetic expression relating light availability in the culture medium with the rate of microalgal growth is obtained. This expression, which is valid for low illumination conditions, was derived from the reactions that take part in the light-dependent stage of photosynthesis. The kinetic expression obtained is a function of the biomass concentration in the culture, as well as of the local volumetric rate of absorption of photons, and only includes two adjustable parameters. To determine the value of these parameters and to test the validity of the hypotheses made, autotrophic cultures of the Chlorella sp. strain were carried out in a modified BBM medium at three CO2 concentrations in the gas stream, namely 0.034%, 0.34% and 3.4%. Moreover, the local volumetric rate of photon absorption was predicted based on a physical model of the interaction of the radiant energy with the suspended biomass, together with a Monte Carlo simulation algorithm. The proposed intrinsic expression of the biomass growth rate, together with the Monte Carlo radiation field simulator, are key to scale up photobioreactors when operating under low irradiation conditions, independently of the configuration of the reactor and of its light source.
[Mh] Termos MeSH primário: Chlorella/crescimento & desenvolvimento
Luz
Modelos Biológicos
[Mh] Termos MeSH secundário: Chlorella/isolamento & purificação
Biologia de Ecossistemas de Água Doce
Fotossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1607
[Cu] Atualização por classe:150904
[Lr] Data última revisão:
150904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150522
[St] Status:MEDLINE
[do] DOI:10.1111/php.12468


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[PMID]:25868573
[Au] Autor:Spyra A; Kubicka J; Strzelec M
[Ad] Endereço:Department of Hydrobiology, The University of Silesia, 9 Bankowa Street, 40-007, Katowice, Poland, aneta.spyra@us.edu.pl.
[Ti] Título:The Influence of the Disturbed Continuity of the River and the Invasive Species--Potamopyrgus antipodarum (Gray, 1843), Gammarus tigrinus (Sexton, 1939) on Benthos Fauna: A Case Study on Urban Area in the River Ruda (Poland).
[So] Source:Environ Manage;56(1):233-44, 2015 Jul.
[Is] ISSN:1432-1009
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The progressive degradation of aquatic ecosystems and ecohydrological role of rivers is one of the most important global environmental issues. The loss of the ability of rivers to self-purify waters due to the disturbances of river continuity cause a lack of biological life in parts of rivers or even in an entire river. The appearance of alien species in degraded aquatic environments is an increasingly common phenomenon and constitutes one of the threats to biodiversity. The aim of the study was to evaluate the possible impact of alien species Potamopyrgus antipodarum (Gray, 1843) and Gammarus tigrinus (Sexton, 1939) on native invertebrates as well as the influence of environmental factors on the occurrence benthos fauna including also alien species. The study conducted in industrial area, in the River Ruda (Poland), showed that at the sites at which the occurrence of the two alien species was observed, the density of native benthos and diversity decreased significantly. CCA analysis showed that non-native species occurred in fast water velocity and that their presence was associated with high values of conductivity, hardness, and a high chloride content. The arrival of new species from other geographical areas is one of the factors that influences the species balance in native aquatic fauna. The number of alien species in freshwater ecosystems probably will increase in the future as new aliens are moved outside of their native ranges.
[Mh] Termos MeSH primário: Organismos Aquáticos
Ecossistema
Espécies Introduzidas
Invertebrados
Rios
[Mh] Termos MeSH secundário: Animais
Biodiversidade
Biologia de Ecossistemas de Água Doce/métodos
Polônia
Densidade Demográfica
Movimentos da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1601
[Cu] Atualização por classe:170924
[Lr] Data última revisão:
170924
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150415
[St] Status:MEDLINE
[do] DOI:10.1007/s00267-015-0483-3


  10 / 58 MEDLINE  
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[PMID]:25356635
[Au] Autor:Cowles MW; Omuro KC; Stanley BN; Quintanilla CG; Zayas RM
[Ad] Endereço:Department of Biology, San Diego State University, San Diego, California, United States of America.
[Ti] Título:COE loss-of-function analysis reveals a genetic program underlying maintenance and regeneration of the nervous system in planarians.
[So] Source:PLoS Genet;10(10):e1004746, 2014 Oct.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Members of the COE family of transcription factors are required for central nervous system (CNS) development. However, the function of COE in the post-embryonic CNS remains largely unknown. An excellent model for investigating gene function in the adult CNS is the freshwater planarian. This animal is capable of regenerating neurons from an adult pluripotent stem cell population and regaining normal function. We previously showed that planarian coe is expressed in differentiating and mature neurons and that its function is required for proper CNS regeneration. Here, we show that coe is essential to maintain nervous system architecture and patterning in intact (uninjured) planarians. We took advantage of the robust phenotype in intact animals to investigate the genetic programs coe regulates in the CNS. We compared the transcriptional profiles of control and coe RNAi planarians using RNA sequencing and identified approximately 900 differentially expressed genes in coe knockdown animals, including 397 downregulated genes that were enriched for nervous system functional annotations. Next, we validated a subset of the downregulated transcripts by analyzing their expression in coe-deficient planarians and testing if the mRNAs could be detected in coe+ cells. These experiments revealed novel candidate targets of coe in the CNS such as ion channel, neuropeptide, and neurotransmitter genes. Finally, to determine if loss of any of the validated transcripts underscores the coe knockdown phenotype, we knocked down their expression by RNAi and uncovered a set of coe-regulated genes implicated in CNS regeneration and patterning, including orthologs of sodium channel alpha-subunit and pou4. Our study broadens the knowledge of gene expression programs regulated by COE that are required for maintenance of neural subtypes and nervous system architecture in adult animals.
[Mh] Termos MeSH primário: Sistema Nervoso Central/crescimento & desenvolvimento
Neurônios
Planárias/genética
Regeneração/genética
[Mh] Termos MeSH secundário: Animais
Biologia de Ecossistemas de Água Doce
Regulação da Expressão Gênica no Desenvolvimento
Técnicas de Silenciamento de Genes
Planárias/crescimento & desenvolvimento
Células-Tronco Pluripotentes
Interferência de RNA
RNA Mensageiro/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Messenger)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141031
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1004746



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