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[PMID]:29212440
[Au] Autor:Gdanetz K; Benucci GMN; Vande Pol N; Bonito G
[Ad] Endereço:Department of Plant Biology, Michigan State University, East Lansing, Michigan, 48824, USA.
[Ti] Título:CONSTAX: a tool for improved taxonomic resolution of environmental fungal ITS sequences.
[So] Source:BMC Bioinformatics;18(1):538, 2017 Dec 06.
[Is] ISSN:1471-2105
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: One of the most crucial steps in high-throughput sequence-based microbiome studies is the taxonomic assignment of sequences belonging to operational taxonomic units (OTUs). Without taxonomic classification, functional and biological information of microbial communities cannot be inferred or interpreted. The internal transcribed spacer (ITS) region of the ribosomal DNA is the conventional marker region for fungal community studies. While bioinformatics pipelines that cluster reads into OTUs have received much attention in the literature, less attention has been given to the taxonomic classification of these sequences, upon which biological inference is dependent. RESULTS: Here we compare how three common fungal OTU taxonomic assignment tools (RDP Classifier, UTAX, and SINTAX) handle ITS fungal sequence data. The classification power, defined as the proportion of assigned OTUs at a given taxonomic rank, varied among the classifiers. Classifiers were generally consistent (assignment of the same taxonomy to a given OTU) across datasets and ranks; a small number of OTUs were assigned unique classifications across programs. We developed CONSTAX (CONSensus TAXonomy), a Python tool that compares taxonomic classifications of the three programs and merges them into an improved consensus taxonomy. This tool also produces summary classification outputs that are useful for downstream analyses. CONCLUSIONS: Our results demonstrate that independent taxonomy assignment tools classify unique members of the fungal community, and greater classification power is realized by generating consensus taxonomy of available classifiers with CONSTAX.
[Mh] Termos MeSH primário: DNA Fúngico/genética
DNA Intergênico/genética
Fungos
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Software
[Mh] Termos MeSH secundário: Microbiologia Ambiental
Fungos/classificação
Fungos/genética
Genoma Fúngico/genética
Genômica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Intergenic)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1186/s12859-017-1952-x


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[PMID]:29291459
[Au] Autor:Quijada NM; Mann E; Wagner M; Rodríguez-Lázaro D; Hernández M; Schmitz-Esser S
[Ad] Endereço:Institute for Milk Hygiene, University of Veterinary Medicine Vienna, Vienna, Austria; Laboratory of Molecular Biology and Microbiology, Instituto Tecnológico Agrario de Castilla y León, Valladolid, Spain.
[Ti] Título:Autochthonous facility-specific microbiota dominates washed-rind Austrian hard cheese surfaces and its production environment.
[So] Source:Int J Food Microbiol;267:54-61, 2018 Feb 21.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cheese ripening involves the succession of complex microbial communities that are responsible for the organoleptic properties of the final products. The food processing environment can act as a source of natural microbial inoculation, especially in traditionally manufactured products. Austrian Vorarlberger Bergkäse (VB) is an artisanal washed-rind hard cheese produced in the western part of Austria without the addition of external ripening cultures. Here, the composition of the bacterial communities present on VB rinds and on different processing surfaces from two ripening cellars was assessed by near full length 16S rRNA gene amplification, cloning and sequencing. Non-inoculated aerobic bacteria dominated all surfaces in this study. VB production conditions (long ripening time, high salt concentration and low temperatures) favor the growth of psychro- and halotolerant bacteria. Several bacterial groups, such as coryneforms, Staphylococcus equorum and Halomonas dominated VB and were also found on most environmental surfaces. Analysis of OTUs shared between different surfaces suggests that VB rind bacteria are inoculated naturally during the ripening from the processing environment and that cheese surfaces exert selective pressure on these communities, as only those bacteria better adapted flourished on VB rinds. This study analyzed VB processing environment microbiota and its relationship with VB rinds for the first time, elucidating that the processing environment and the cheese microbiota should be considered as microbiologically linked ecosystems with the goal of better defining the events that take place during cheese maturation.
[Mh] Termos MeSH primário: Fenômenos Fisiológicos Bacterianos
Queijo/microbiologia
Microbiologia Ambiental
[Mh] Termos MeSH secundário: Áustria
Bactérias/genética
Manipulação de Alimentos
Microbiota/genética
Microbiota/fisiologia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180102
[St] Status:MEDLINE


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[PMID]:28452235
[Au] Autor:Mizan MFR; Bang HJ; Sadekuzzaman M; Lee N; Kim TJ; Ha SD
[Ad] Endereço:a Department of Food Science and Technology, Advanced Food Safety Research group, Brain Korea 21 Plus , Chung-Ang University , Anseong , Republic of Korea.
[Ti] Título:Molecular characteristics, biofilm-forming abilities, and quorum sensing molecules in Vibrio parahaemolyticus strains isolated from marine and clinical environments in Korea.
[So] Source:Biofouling;33(5):369-378, 2017 05.
[Is] ISSN:1029-2454
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Vibrio parahaemolyticus is an inhabitant of marine and estuarine environments and causes seafood-borne gastroenteritis in humans. In this study, an UltraFast LabChip Real-Time PCR assay was evaluated for rapid detection and quantification of pathogenic V. parahaemolyticus isolates. Escherichia coli and Vibrio harveyi were used as negative controls. Twenty-six tdh-positive, biofilm-producing V. parahaemolyticus isolates were analyzed by repetitive extragenic palindromic-polymerase chain reaction (REP-PCR). REP-PCR analysis showed that the majority of the V. parahaemolyticus isolates originated from seafood and that clinical specimens formed two major clusters at 92.8% and 32% similarity levels. The presence and quantification of Autoinducer-2 was carried out using high-performance liquid chromatography with fluorescence detection (HPLC-FLD) after derivatization of Autoinducer-2 with 2, 3-diaminonaphthalene. The presence of tdh-positive V. parahaemolyticus in marine samples highlights the need for constant environmental monitoring to protect public health.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Técnicas de Laboratório Clínico/métodos
Microbiologia Ambiental
Percepção de Quorum
Alimentos Marinhos/microbiologia
Vibrio parahaemolyticus/isolamento & purificação
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/métodos
Microbiologia Ambiental/normas
Microbiologia de Alimentos/métodos
Doenças Transmitidas por Alimentos/microbiologia
Doenças Transmitidas por Alimentos/prevenção & controle
Seres Humanos
Reação em Cadeia da Polimerase em Tempo Real/métodos
República da Coreia
Sensibilidade e Especificidade
Especificidade da Espécie
Vibrio parahaemolyticus/genética
Vibrio parahaemolyticus/patogenicidade
Vibrio parahaemolyticus/fisiologia
Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1080/08927014.2017.1316840


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[PMID]:29327895
[Au] Autor:Savic D; Miljkovic-Selimovic B; Lepsanovic Z; Tambur Z; Konstantinovic S; Stankovic N; Ristanovic E
[Ti] Título:Antimicrobial susceptibility and ß-lactamase production in Bacillus cereus isolates from stool of patients, food and environment samples.
[So] Source:Vojnosanit Pregl;73(10):904-9, 2016 Oct.
[Is] ISSN:0042-8450
[Cp] País de publicação:Serbia
[La] Idioma:eng
[Ab] Resumo:Background/Aim: Bacillus cereus (B. cereus) usually ingested by food can cause two types of diseases: vomiting due to the presence of emetic toxin and diarrheal syndrome, due to the presence of diarrheal toxins. Systemic manifestations can also occur. The severe forms of disease demand antibiotic treatmant. The aim of this study was to determine the differences in antibiotic susceptibility and ß-lactamase activity of B. cereus isolates from stools of humans, food and environment. Methods: Identification of B. cereus was performed with selective medium, classical biochemical test and polymerase chain reaction (PCR) with primers specific for bal gene. Thirty isolates from each group were analysed for antibiotic susceptibility using the disk-diffusion assay. Production of ß-lactamase was determined by cefinase test, and double-disc method. Results: All strains identified as B. cereus using classical biochemical test, yielded 533 bp fragment with PCR. Isolates from all the three groups were susceptible to imipenem, vancomycin, and erythromycin. All isolates were susceptible to ciprofloxacin but one from the environment. A statistically significant difference between the groups was confirmed to tetracycline and trimethoprim-sulphamethoxazole sensitivity. A total of 28/30 (93.33%) samples from the foods and 25/30 (83.33%) samples from environment were approved sensitive to tetracycline, while 10/30 (33.33%) isolates from stools were sensitive. Opposite to this result, high susceptibility to trimethoprim-sulphamethoxazole was shown in samples from stools (100%), while isolates from foods (63.33%) and from environment (70%) had low susceptibility. All samples produced ß-lactamases. Conclusion: The strains of B. cereus from all the three groups showed high rate of sensitivity to most tested antibiotics, except to tetracycline in samples from human stool and to trimethoprim-sulphamethoxazole in samples from food and environment. The production of ß-lactamases was confirmed in all the strains.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bacillus cereus/efeitos dos fármacos
Farmacorresistência Bacteriana
Microbiologia Ambiental
Fezes/microbiologia
beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Bacillus cereus/enzimologia
Bacillus cereus/genética
Bacillus cereus/isolamento & purificação
Microbiologia de Alimentos
Seres Humanos
Testes de Sensibilidade Microbiana
beta-Lactamases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.2298/VSP150415134S


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[PMID]:29217420
[Au] Autor:Párraga-Niño N; Quero S; Uria N; Castillo-Fernandez O; Jimenez-Ezenarro J; Muñoz FX; Sabrià M; Garcia-Nuñez M
[Ad] Endereço:Unitat de Malalties Infeccioses, Fundació Institut d'Investigació Germans Trias I Pujol, Badalona, Spain E-08916; CIBER de Enfermedades Respiratorias, Madrid, Spain E-28029.
[Ti] Título:Antibody test for Legionella pneumophila detection.
[So] Source:Diagn Microbiol Infect Dis;90(2):85-89, 2018 Feb.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Legionella pneumophila is responsible for Legionnaires' disease (LD). Its detection in both environmental and clinical samples is mainly performed by culture plate method which requires up to 10days to obtain results. Nowadays, there are commercial antibodies against this bacterium, but they have not been tested against all subgroups of L. pneumophila sg 1 or serogroups 1-16 or their cross-reactions with other non-Legionella bacteria. Indeed, many of these antibodies became available when only 8 serogroups of L. pneumophila had been described. We tested 7 antibodies and found that 2 (Mab 8/5 and OBT) specifically detected all the subgroups of L. pneumophila sg 1, one without cross-reactions (Mab8/5). Moreover, the LP3IIG2 antibody detected almost all serogroups tested with lower rates of cross-reactivity, resulting in a specific sensitive antibody for the detection of L. pneumophila. LP3IIG2 presented higher rate of cross-reactivity against respiratory non-Legionella isolates, thereby contraindicating its clinical applicability.
[Mh] Termos MeSH primário: Anticorpos Antibacterianos/imunologia
Técnicas de Tipagem Bacteriana/métodos
Legionella pneumophila/imunologia
Doença dos Legionários/microbiologia
Sorotipagem/métodos
[Mh] Termos MeSH secundário: Antígenos de Bactérias/análise
Antígenos de Bactérias/imunologia
Microbiologia Ambiental
Técnica Indireta de Fluorescência para Anticorpo
Seres Humanos
Legionella pneumophila/isolamento & purificação
Doença dos Legionários/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antigens, Bacterial)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE


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[PMID]:29029976
[Au] Autor:Martín-Díaz J; Lucena F
[Ad] Endereço:Department of Genetics, Microbiology and Statistics, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain; The Water Research Institute, University of Barcelona, C/Montalegre 6, 08001 Barcelona, Spain. Electronic address: juliamadi@hotmail.com.
[Ti] Título:Extraction and RT-qPCR detection of enteroviruses from solid environmental matrixes: Method decision tree for different sample types and viral concentrations.
[So] Source:J Virol Methods;251:145-150, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Quantitative RT-PCR methods (RT-qPCR) are becoming increasingly desirable for the detection of enteric viruses in solid environmental matrixes such as sediments, soils and sewage sludge. However, effective methodologies that allow the extraction of high quality RNA ready for molecular quantification continue to be evaluated. In the present study, four different methods for enterovirus extraction from solid environmental matrixes were compared in terms of viral recovery and inhibitor removal. Three indirect methods based on glycine elution and concentration by ultracentrifugation were tested. The main differences between indirect methods were the sample to glycine buffer ratio, and the ultracentrifugation protocol applied. One commercial direct method was also tested. The indirect methods produced better results than the direct method. The ultracentrifugation led to viral losses in samples with high titers; however, as the virus concentration reduced, the ultracentrifugation became increasingly important for viral recovery. Two commercial RNA extraction kits were also evaluated and it was selected the most effective in removing RT-qPCR inhibitors. The results obtained allowed the development of a method decision tree with three versions that are suitable for different samples and viral concentrations.
[Mh] Termos MeSH primário: Enterovirus/isolamento & purificação
Microbiologia Ambiental
RNA Viral/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real/métodos
Carga Viral/métodos
[Mh] Termos MeSH secundário: Árvores de Decisões
Enterovirus/genética
RNA Viral/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171015
[St] Status:MEDLINE


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[PMID]:28457900
[Au] Autor:Bardbari AM; Arabestani MR; Karami M; Keramat F; Alikhani MY; Bagheri KP
[Ad] Endereço:Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address: Alimoh2412@gmail.com.
[Ti] Título:Correlation between ability of biofilm formation with their responsible genes and MDR patterns in clinical and environmental Acinetobacter baumannii isolates.
[So] Source:Microb Pathog;108:122-128, 2017 Jul.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Acinetobacter baumannii potential to form biofilm and exhibit multiple antibiotic resistances may be responsible in its survival in hospital environment. Accordingly, our study was aimed to determine the correlation between ability of biofilm formation and the frequency of biofilm related genes with antibiotic resistance phenotypes, and also the categorization of their patterns in clinical and environmental isolates. A total of 75 clinical and 32 environmental strains of the A. baumannii were collected and identified via API 20NE. Antibiotic susceptibility was evaluated by disk diffusion and microdilution broth methods. Biofilm formation assay was performed by microtiter plate method. OXA types and biofilm related genes including Bla , Bla , Bla , Bla , bap, bla , and ompA were amplified by PCR. The rate of MDR A. baumannii in clinical isolates (100%) was higher than environmental (81.2%) isolates (p < 0.05). Among 10 antibiotypes, the predominant resistance pattern in clinical and environmental isolates was antibiotypes I (85.3 and 78.1%, respectively). Analysis of the frequency of bla gene revealed a statistically significant difference between clinical (85.3%) and environmental (68.7%) isolates (p < 0.05). The prevalence of strong biofilm producers in clinical and environmental isolates were 31.2%-58.7%, respectively. In the clinical and environmental isolates, the frequencies of ompA, bla and bap genes were 100%, 53.3%, 82.7% and 100%, 37.5%, 84.4% respectively. Statistical analysis revealed a significant correlation between the frequency of MDR isolates and biofilm formation ability (p = 0.008). The high frequency of antibiotype I would be indicated that an outbreak has been happened earlier and an endemic strain is currently being settled in the hospital environment. It would be suggested that if there was no difference in the frequency of pattern I and biofilm formation ability between clinical and environmental isolates, it is a critical point representing the higher risk of bacterial transmission from environment to the patients. The resulting data would be assisted in the improvement of disinfection strategies to better control of nosocomial infections. One dominant resistance pattern has shown among clinical and environmental isolates. The frequency of blaOXA-23 had significant difference between clinical and environmental isolates. The presence of bap gene in the A. baumannii isolates was associated with biofilm formation. There was a significant correlation between multiple drug resistance and biofilm formation. The clinical isolates had a higher ability to form strong biofilms compared to the environmental samples.
[Mh] Termos MeSH primário: Acinetobacter baumannii/genética
Acinetobacter baumannii/fisiologia
Biofilmes/crescimento & desenvolvimento
Genes MDR/genética
Genes MDR/fisiologia
[Mh] Termos MeSH secundário: Infecções por Acinetobacter/microbiologia
Acinetobacter baumannii/efeitos dos fármacos
Acinetobacter baumannii/isolamento & purificação
Adulto
Idoso
Idoso de 80 Anos ou mais
Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Técnicas Bacteriológicas
Biofilmes/efeitos dos fármacos
Infecção Hospitalar/microbiologia
Infecção Hospitalar/prevenção & controle
DNA Bacteriano/análise
Farmacorresistência Bacteriana Múltipla/genética
Microbiologia Ambiental
Feminino
Seres Humanos
Irã (Geográfico)
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (DNA, Bacterial)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:29214970
[Au] Autor:Kassi FK; Bellet V; Drakulovski P; Krasteva D; Roger F; Valérie BA; Aboubakar T; Doumbia A; Kouakou GA; Delaporte E; Reynes J; Yavo W; Menan HIE; Bertout S
[Ad] Endereço:1​Laboratoire de Parasitologie et de Mycologie - CeDReS (Centre de Diagnostic et de Recherche sur le SIDA et les Autres Maladies Infectieuses), UFR Pharmacie, CHU de Treichville, Université Félix Houphouët Boigny, Abidjan, Ivory Coast.
[Ti] Título:Comparative typing analyses of clinical and environmental strains of the Cryptococcus neoformans/Cryptococcus gattii species complex from Ivory Coast.
[So] Source:J Med Microbiol;67(1):87-96, 2018 Jan.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The aim of this study was to assess the biotope of the Cryptococcus neoformans/Cryptococcus gattii species complex from Ivory Coast, and clarify the possible epidemiological relationship between environmental and clinical strains. METHODOLOGY: Samples from Eucalyptus camaldulensis (n=136), Mangifera indica (n=13) and pigeon droppings (n=518) were collected from different sites close to the living environment of Ivorian HIV patients with cryptococcosis (n=10, 50 clinical strains). Clinical and environmental strains were characterized by molecular serotyping and genotyping [RFLP analysis of the URA5 gene, (GACA)4, (GTG)5 and M13 PCR fingerprinting] and compared.Results/Key findings. Environmental strains were recovered only from the pigeon droppings. In vitro susceptibility profiles showed that all strains were susceptible to fluconazole, flucytosine and amphotericin B. All environmental strains consisted of C. neoformans (A, AFLP1/VNI), whereas clinical strains included C. neoformans (A, AFLP1/VNI), C. neoformans x Cryptococcus deneoformans hybrids (AD, AFLP3/VNIII) and Cryptococcus deuterogattii (B, AFLP6/VGII). Two patients were co-infected with both C. neoformans and C. neoformans x C. deneoformans hybrids. We noticed a low genetic diversity among the environmental samples compared to the high diversity of the clinical samples. Some clinical strains were genetically more similar to environmental strains than to other clinical strains, including those from the same patient. CONCLUSION: These results provide new information on the ecology and epidemiology of the C. neoformans/C. gattii species complex in Ivory Coast.
[Mh] Termos MeSH primário: Cryptococcus gattii/genética
Cryptococcus neoformans/genética
[Mh] Termos MeSH secundário: Adulto
Anfotericina B/uso terapêutico
Antifúngicos/uso terapêutico
Cloranfenicol/uso terapêutico
Costa do Marfim
Criptococose/microbiologia
DNA Fúngico/genética
Microbiologia Ambiental
Feminino
Fluconazol/uso terapêutico
Flucitosina/uso terapêutico
Genótipo
Infecções por HIV/microbiologia
Seres Humanos
Masculino
Testes de Sensibilidade Microbiana/métodos
Meia-Idade
Tipagem Molecular/métodos
Técnicas de Tipagem Micológica/métodos
Estudos Prospectivos
Sorotipagem/métodos
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (DNA, Fungal); 66974FR9Q1 (Chloramphenicol); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); D83282DT06 (Flucytosine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000654


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[PMID]:29211242
[Au] Autor:Castro-Artavia E; Retana-Moreira L; Lorenzo-Morales J; Abrahams-Sandí E
[Ad] Endereço:University of Costa Rica, Faculty of Microbiology, Department of Parasitology, San Pedro, San José, Costa Rica.
[Ti] Título:Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers.
[So] Source:Mem Inst Oswaldo Cruz;112(12):817-821, 2017 Dec.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Acanthamoeba is the genus of free-living amoebae that is most frequently isolated in nature. To date, 20 Acanthamoeba genotypes have been described. Genotype T4 is responsible for approximately 90% of encephalitis and keratitis cases. Due to the ubiquitous presence of amoebae, isolation from environmental sources is not uncommon; to determine the clinical importance of an isolation, it is necessary to have evidence of the pathogenic potential of amoebae. OBJECTIVE: The aim of this study was to physiologically characterise 8 Acanthamoeba T4 isolates obtained from dental units and emergency combination showers and to determine their pathogenic potential by employing different laboratory techniques. METHODS: Eight axenic cultures of Acanthamoeba genotype T4 were used in pathogenic potential assays. Osmotolerance, thermotolerance, determination and characterisation of extracellular proteases and evaluation of cytopathic effects in MDCK cells were performed. FINDINGS: All of the isolates were osmotolerant, thermotolerant and had serine proteases from 44-122 kDa. Two isolates had cytopathic effects on the MDCK cell monolayer. MAIN CONCLUSION: The presence of Acanthamoeba T4 with pathogenic potential in areas such as those tested in this study reaffirms the need for adequate cleaning and maintenance protocols to reduce the possibility of infection with free-living amoebae.
[Mh] Termos MeSH primário: Acanthamoeba
Microbiologia Ambiental
[Mh] Termos MeSH secundário: Acanthamoeba/genética
Acanthamoeba/isolamento & purificação
Acanthamoeba/patogenicidade
Genótipo
Seres Humanos
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


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[PMID]:28470425
[Au] Autor:Ranawat P; Rawat S
[Ad] Endereço:Department of Botany and Microbiology, Hemvati Nandan Bahuguna Garhwal University, Srinagar (Garhwal), Uttrakhand, India.
[Ti] Título:Radiation resistance in thermophiles: mechanisms and applications.
[So] Source:World J Microbiol Biotechnol;33(6):112, 2017 Jun.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The study of prokaryotic life in high temperature environments viz., geothermal areas, hot, acidic geysers and undersea hydrothermal vents has revealed the existence of thermophiles (or hyperthermophiles). These microorganisms possess various stress adaptation mechanisms which enable them to bypass multiple physical and chemical barriers for survival. The discovery of radiation resistant thermophile Deinococcus geothermalis has given new insights into the field of radiation microbiology. The ability of radiation resistant thermophiles to deal with the lethal effects of ionizing radiations like DNA damage, oxidative bursts and protein damage has made them a model system for exobiology and interplanetary transmission of life. They might be an antiquity of historical transport process that brought microbial life on Earth. These radiation resistant thermophiles are resistant to desiccation as well and maintain their homeostasis by advance DNA repair mechanisms, reactive oxygen species (ROS) detoxification system and accumulation of compatible solutes. Moreover, engineered radioresistant thermophilic strains are the best candidate for bioremediation of radionuclide waste while the extremolytes produced by these organisms may have predicted therapeutic uses. So, the present article delineate a picture of radiation resistance thermophiles, their adaptive mechanisms to evade stress viz., radiation and desiccation, their present applications along with new horizons in near future.
[Mh] Termos MeSH primário: Archaea/fisiologia
Archaea/efeitos da radiação
Bactérias/efeitos da radiação
Fenômenos Fisiológicos Bacterianos/efeitos da radiação
Temperatura Alta
[Mh] Termos MeSH secundário: Actinobacteria/fisiologia
Actinobacteria/efeitos da radiação
Bactérias/genética
Fenômenos Fisiológicos Bacterianos/genética
Biodegradação Ambiental
Cianobactérias/fisiologia
Cianobactérias/efeitos da radiação
Dano ao DNA/efeitos da radiação
Reparo do DNA
Deinococcus/genética
Deinococcus/fisiologia
Deinococcus/efeitos da radiação
Microbiologia Ambiental
Exobiologia
Halobacterium/fisiologia
Halobacterium/efeitos da radiação
Pyrococcus/fisiologia
Pyrococcus/efeitos da radiação
Radiação Ionizante
Espécies Reativas de Oxigênio/efeitos da radiação
Explosão Respiratória/efeitos da radiação
Estresse Fisiológico
Sulfolobus/fisiologia
Sulfolobus/efeitos da radiação
Thermococcus/fisiologia
Thermococcus/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Reactive Oxygen Species)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-017-2279-5



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