[PMID]: | 27129880 |
[Au] Autor: | Yang CL; Wu XP; Chen B; Deng SS; Chen ZE; Huang YY; Jin SS |
[Ad] Endereço: | Institute of Agricultural Engineering Technology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China. |
[Ti] Título: | Comparative analysis of genetic polymorphisms among Monascus strains by ISSR and RAPD markers. |
[So] Source: | J Sci Food Agric;97(2):636-640, 2017 Jan. |
[Is] ISSN: | 1097-0010 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | BACKGROUND: The genus Monascus includes several species of fungi valued across Asia for their culinary uses and diverse medicinal properties. In this study, we evaluated the applicability of random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers in characterizing the genetic diversity in 41 Monascus strains collected from various regions of Fujian Province, the leading producer of Monascus in China. RESULTS: Seven screened ISSR primers generated 56 polymorphic bands, of which 93.33% were polymorphic. The genetic similarity coefficients (GSC) of the strains ranged from 0.50 to 1.00. Comparative sequence analysis using seven screened RAPD primers amplified a total of 49 polymorphic bands, of which 81.67% were polymorphic; GSC values ranged from 0.62 to 1.00. CONCLUSION: Correlation analysis revealed a significant positive correlation in genetic distances assessed using above two markers, which indicated they were suitable for Monascus species characterization. ISSR markers were more suitable for the classification and determination of Monascus species, while RAPD markers appear to be preferable for analyzing the differences among strains within the same species. Our study revealed that Monascus possesses rich genetic diversity, and that the genetic relationships among the selected strains were, to a very limited extent, correlated to their geographical variation. © 2016 Society of Chemical Industry. |
[Mh] Termos MeSH primário: |
Monascus/genética Polimorfismo Genético
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[Mh] Termos MeSH secundário: |
China Análise por Conglomerados DNA Fúngico/química DNA Fúngico/metabolismo Marcadores Genéticos Monascus/classificação Monascus/crescimento & desenvolvimento Monascus/isolamento & purificação Micologia/métodos Filogenia Filogeografia/métodos Técnica de Amplificação ao Acaso de DNA Polimórfico Sequências Repetitivas de Ácido Nucleico
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[Pt] Tipo de publicação: | COMPARATIVE STUDY; JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (DNA, Fungal); 0 (Genetic Markers) |
[Em] Mês de entrada: | 1706 |
[Cu] Atualização por classe: | 170613 |
[Lr] Data última revisão:
| 170613 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 160501 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1002/jsfa.7780 |
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