Base de dados : MEDLINE
Pesquisa : H01.158.273.540.859 [Categoria DeCS]
Referências encontradas : 5135 [refinar]
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[PMID]:28450173
[Au] Autor:Chandler JC; Schaeffer JW; Davidson M; Magzamen SL; Pérez-Méndez A; Reynolds SJ; Goodridge LD; Volckens J; Franklin AB; Shriner SA; Bisha B
[Ad] Endereço:National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, CO, USA.
[Ti] Título:A method for the improved detection of aerosolized influenza viruses and the male-specific (F+) RNA coliphage MS2.
[So] Source:J Virol Methods;246:38-41, 2017 Aug.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The detection of aerosolized viruses can serve as an important surveillance and control tool in agriculture, human health, and environmental settings. Here, we adapted an anion exchange resin-based method, initially developed to concentrate negatively charged viruses from water, to liquid impingement-based bioaerosol sampling. In this method, aerosolized viruses are collected in a 20ml liquid sample contained within widely used impingers, BioSamplers (SKC Inc., Eighty Four, PA), and further concentrated via adsorption to an anion exchange resin that is suspended within this liquid. Viral nucleic acids are then extracted from the resin to facilitate molecular analyses through a reduction in the effective sample volume. For this study, various quantities of two negatively charged viruses, type A and type B influenza viruses (FluMist Quadrivalent vaccine) and the male-specific (F+) RNA coliphage MS2 (MS2), were nebulized into a custom-built bioaerosolization chamber, and sampled using BioSamplers with and without anion exchange resin. Compared to direct testing of the BioSampler liquid, detection was improved by 6.77× and 3.33× for type A and type B influenza viruses, respectively, by using the anion exchange resin. For MS2, the anion exchange resin method allowed for an average improvement in detection of 8.26×.
[Mh] Termos MeSH primário: Microbiologia do Ar
Levivirus/isolamento & purificação
Orthomyxoviridae/isolamento & purificação
Virologia/métodos
[Mh] Termos MeSH secundário: Aerossóis
Resinas de Troca de Ânions
Seres Humanos
Levivirus/genética
Masculino
RNA Viral
Manejo de Espécimes/métodos
Virologia/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aerosols); 0 (Anion Exchange Resins); 0 (RNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  2 / 5135 MEDLINE  
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[PMID]:29381929
[Au] Autor:Röck T; Beck R; Jürgens S; Bartz-Schmidt KU; Bramkamp M; Thaler S; Röck D
[Ad] Endereço:Centre for Ophthalmology.
[Ti] Título:Factors influencing the virological testing of cornea donors.
[So] Source:Medicine (Baltimore);96(47):e8561, 2017 Nov.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To assess the influence of donor, environment, and logistical factors on the results of virological testing of blood samples from cornea donors.Data from 670 consecutive cornea donors were analyzed retrospectively. Logistic regression analysis was used to assess the influence of different factors on the results of virological testing of blood samples from cornea donors.The mean annual rate of donors with serology-reactive or not evaluable result was 14.8% (99 of 670) (range 11.9%-16.9%). The cause of donor death by cancer increased the risk of serology-reactive or not evaluable result (P = .0300). Prolonged time between death and post mortem blood removal was associated with a higher rate of serology-reactive or not evaluable result (P < .0001). Mean monthly temperature including warmer months, differentiating between septic and aseptic donors, sex, and donor age had no significant impact on the results of virological testing of blood samples from cornea donors.The cause of donor death by cancer and a prolonged time between death and post mortem blood removal seem to be mainly responsible for serology-reactive or not evaluable result of blood samples from cornea donors. The percentage of discarded corneas caused by serology-reactive or not evaluable result may be reduced by shortening the period of time between death and post mortem blood removal.
[Mh] Termos MeSH primário: Transplante de Córnea/métodos
Doadores de Tecidos
Virologia/métodos
Virologia/estatística & dados numéricos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Fatores Etários
Idoso
Idoso de 80 Anos ou mais
Causas de Morte
Feminino
Seres Humanos
Modelos Logísticos
Masculino
Meia-Idade
Estudos Retrospectivos
Fatores Sexuais
Fatores de Tempo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008561


  3 / 5135 MEDLINE  
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[PMID]:29198371
[Au] Autor:François C; Segard C; Bouvier M; Stefanski M; Pannier C; Zawadzki P; Roussel C; Hecquet D; Duverlie G; Brochot E; Castelain S
[Ad] Endereço:Laboratoire de Virologie, CHU d'Amiens, France; EA 4294, Unité de virologie clinique et fondamentale, Université Picardie Jules Verne, Amiens, France. Electronic address: catherine.francois@u-picardie.fr.
[Ti] Título:Comparison of Abbott Architect , Siemens Immulite , and Diasorin Liaison for determination of Epstein-Barr virus serological diagnosis.
[So] Source:Diagn Microbiol Infect Dis;90(2):96-101, 2018 Feb.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study compared the performance of 3 automated immunoassays, Architect (Abbott), Immulite (Siemens) and Liaison (Diasorin), for Epstein-Barr virus (EBV) serology. Ninety-one serum samples collected in Amiens University Hospital were analyzed for the presence of Viral Capsid Antigen (VCA) IgG and IgM and Epstein-Barr Nuclear Antigen (EBNA) IgG. The agreement between the 3 assays was calculated for each marker individually and for determination of the EBV profile, based on interpretation of the combination of these 3 EBV markers. Although similar results were obtained with Architect and Liaison , several discordant results were observed with Immulite , particularly for EBNA IgG. A large number of EBNA IgG-positive results were observed, which interfered with interpretation of the EBV profile. In contrast, Immulite performed similarly to the 2 other assays for detection of VCA IgM.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Antígenos Virais/sangue
Infecções por Vírus Epstein-Barr/diagnóstico
Herpesvirus Humano 4/imunologia
Imunoensaio/métodos
[Mh] Termos MeSH secundário: Infecções por Vírus Epstein-Barr/virologia
Herpesvirus Humano 4/isolamento & purificação
Seres Humanos
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Estudos Retrospectivos
Sensibilidade e Especificidade
Virologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (Immunoglobulin G); 0 (Immunoglobulin M)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


  4 / 5135 MEDLINE  
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[PMID]:29074234
[Au] Autor:Sasaki M; Anindita PD; Phongphaew W; Carr M; Kobayashi S; Orba Y; Sawa H
[Ad] Endereço:Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.
[Ti] Título:Development of a rapid and quantitative method for the analysis of viral entry and release using a NanoLuc luciferase complementation assay.
[So] Source:Virus Res;243:69-74, 2018 01 02.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Subviral particles (SVPs) self-assemble and are released from cells transfected with expression plasmids encoding flavivirus structural proteins. Flavivirus-like particles (VLPs), consisting of flavivirus structural proteins and a subgenomic replicon, can enter cells and cause single-round infections. Neither SVPs or VLPs possess complete viral RNA genomes, therefore are replication-incompetent systems; however, they retain the capacity to fuse and bud from target cells and follow the same maturation process as whole virions. SVPs and VLPs have been previously employed in studies analyzing entry and release steps of viral life cycles. In this study, we have developed quantitative methods for the detection of cellular entry and release of SVPs and VLPs by applying a luciferase complementation assay based on the high affinity interaction between the split NanoLuc luciferase protein, LgBiT and the small peptide, HiBiT. We introduced HiBiT into the structural protein of West Nile virus and generated SVPs and VLPs harboring HiBiT (SVP-HiBiT and VLP-HiBiT, respectively). As SVP-HiBiT emitted strong luminescence upon exposure to LgBiT and its substrate, the nascently budded SVP-HiBiT in the supernatant was readily quantified by luminometry. Similarly, the cellular entry of VLP-HiBiT generated luminescence when VLP-HiBiT was infected into LgBiT-expressing cells. These methods utilizing SVP-HiBiT and VLP-HiBiT will facilitate research into life cycles of flaviviruses, including WNV.
[Mh] Termos MeSH primário: Luciferases/análise
Vírion/fisiologia
Virologia/métodos
Internalização do Vírus
Liberação de Vírus
Vírus do Nilo Ocidental/fisiologia
[Mh] Termos MeSH secundário: Linhagem Celular
Seres Humanos
Luciferases/genética
Luciferases/metabolismo
Vírion/genética
Febre do Nilo Ocidental/virologia
Vírus do Nilo Ocidental/genética
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171028
[St] Status:MEDLINE


  5 / 5135 MEDLINE  
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[PMID]:29050971
[Au] Autor:Zhou C; Li S; Zhou Y; Fan Y
[Ad] Endereço:College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
[Ti] Título:A simple method for obtaining rice black-streaked dwarf virus-infected small brown planthopper nymphs.
[So] Source:J Virol Methods;251:80-82, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Rice black-streaked dwarf virus (RBSDV), an important rice virus, is transmitted by vector small brown planthopper (SBPH) in a persistent manner, but not transovarial transmission. In order to obtain viruliferous SBPH nymphs for relevant research, a simple and reliable method was developed, through allowing SBPH adults laying eggs on RBSDV-infected rice plants. The results showed the hatching nymphs on diseased plants could early acquire virus, and the virus was detected in 2nd-instar nymphs from the spawning method, which was earlier than insect feed on diseased plant. The average viruliferous rate of SBPH from the spawning method was 32.9%, which was not lower than the feeding diseased plant method. The novel method was very easy to operate and time-saving, facilitating the study on the interaction between RBSDV and SBPH nymphs (especially young 2nd-4th instar nymphs), such as, the effect of RBSDV on nymph development, host plant orientation preference of viruliferous nymph, identification of viral interacting protein in nymph, etc.
[Mh] Termos MeSH primário: Entomologia/métodos
Hemípteros/virologia
Ninfa/virologia
Vírus de Plantas/crescimento & desenvolvimento
Virologia/métodos
[Mh] Termos MeSH secundário: Animais
Hemípteros/crescimento & desenvolvimento
Ninfa/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171021
[St] Status:MEDLINE


  6 / 5135 MEDLINE  
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[PMID]:28966037
[Au] Autor:Allmaier G; Blaas D; Bliem C; Dechat T; Fedosyuk S; Gösler I; Kowalski H; Weiss VU
[Ad] Endereço:Institute of Chemical Technologies and Analytics, TU Wien (Vienna University of Technology), Vienna, Austria.
[Ti] Título:Monolithic anion-exchange chromatography yields rhinovirus of high purity.
[So] Source:J Virol Methods;251:15-21, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:For vaccine development, 3D-structure determination, direct fluorescent labelling, and numerous other studies, homogeneous virus preparations of high purity are essential. Working with human rhinoviruses (RVs), members of the picornavirus family and the main cause of generally mild respiratory infections, we noticed that our routine preparations appeared highly pure on analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), exclusively showing the four viral capsid proteins (VPs). However, the preparations turned out to contain substantial amounts of contaminating material when analyzed by orthogonal analytical methods including capillary zone electrophoresis, nano electrospray gas-phase electrophoretic mobility molecular analysis (nES GEMMA), and negative stain transmission electron microscopy (TEM). Because these latter analyses are not routine to many laboratories, the above contaminations might remain unnoticed and skew experimental results. By using human rhinovirus serotype A2 (RV-A2) as example we report monolithic anion-exchange chromatography (AEX) as a last polishing step in the purification and demonstrate that it yields infective, highly pure, virus (RV-A2 in the respective fractions was confirmed by peptide mass fingerprinting) devoid of foreign material as judged by the above criteria.
[Mh] Termos MeSH primário: Cromatografia por Troca Iônica/métodos
Rhinovirus/isolamento & purificação
Virologia/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE


  7 / 5135 MEDLINE  
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[PMID]:27775563
[Au] Autor:Dirk BS; Van Nynatten LR; Dikeakos JD
[Ad] Endereço:Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 5C1, Canada. bdirk@uwo.ca.
[Ti] Título:Where in the Cell Are You? Probing HIV-1 Host Interactions through Advanced Imaging Techniques.
[So] Source:Viruses;8(10), 2016 10 19.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Viruses must continuously evolve to hijack the host cell machinery in order to successfully replicate and orchestrate key interactions that support their persistence. The type-1 human immunodeficiency virus (HIV-1) is a prime example of viral persistence within the host, having plagued the human population for decades. In recent years, advances in cellular imaging and molecular biology have aided the elucidation of key steps mediating the HIV-1 lifecycle and viral pathogenesis. Super-resolution imaging techniques such as stimulated emission depletion (STED) and photoactivation and localization microscopy (PALM) have been instrumental in studying viral assembly and release through both cell-cell transmission and cell-free viral transmission. Moreover, powerful methods such as Forster resonance energy transfer (FRET) and bimolecular fluorescence complementation (BiFC) have shed light on the protein-protein interactions HIV-1 engages within the host to hijack the cellular machinery. Specific advancements in live cell imaging in combination with the use of multicolor viral particles have become indispensable to unravelling the dynamic nature of these virus-host interactions. In the current review, we outline novel imaging methods that have been used to study the HIV-1 lifecycle and highlight advancements in the cell culture models developed to enhance our understanding of the HIV-1 lifecycle.
[Mh] Termos MeSH primário: HIV-1/fisiologia
HIV-1/patogenicidade
Interações Hospedeiro-Patógeno
Imagem Óptica/métodos
Virologia/métodos
[Mh] Termos MeSH secundário: Células Cultivadas
Seres Humanos
Coloração e Rotulagem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171212
[Lr] Data última revisão:
171212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  8 / 5135 MEDLINE  
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[PMID]:28863444
[Au] Autor:Datta S; Walsh EE; Peterson DR; Falsey AR
[Ad] Endereço:Department of Medicine, Rochester General Hospital.
[Ti] Título:Can Analysis of Routine Viral Testing Provide Accurate Estimates of Respiratory Syncytial Virus Disease Burden in Adults?
[So] Source:J Infect Dis;215(11):1706-1710, 2017 Jun 01.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Respiratory syncytial virus (RSV) is increasingly recognized as a significant cause of adult respiratory illness. We evaluated routine viral testing and discharge diagnoses for identifying RSV and influenza burden. Polymerase chain reaction results performed in adults during emergency room visits or hospitalizations were reviewed. Peak RSV activity preceded influenza activity by 8 weeks. The ratio of total number of viral tests performed divided by total number of respiratory visits was higher during influenza than RSV peaks (1.31 vs 0.72; P = .0001). Influenza and RSV were listed primary diagnoses in 56 (30%) vs 7 (6%), respectively (P < .0001). Routine viral testing to estimate adult RSV disease burden has limitations.
[Mh] Termos MeSH primário: Testes Diagnósticos de Rotina/estatística & dados numéricos
Infecções por Vírus Respiratório Sincicial/diagnóstico
Infecções por Vírus Respiratório Sincicial/epidemiologia
Vírus Sincicial Respiratório Humano
Virologia/estatística & dados numéricos
[Mh] Termos MeSH secundário: Adulto
Seres Humanos
Influenza Humana
Reação em Cadeia da Polimerase
Infecções por Vírus Respiratório Sincicial/virologia
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170903
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix196


  9 / 5135 MEDLINE  
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[PMID]:28814703
[Au] Autor:Shin M
[Ad] Endereço:Dept. of Science Studies, Chonbuk National University, Jeonju-si, Jeollabuk-do, KOREA.
[Ti] Título:Becoming an International Scientist in South Korea: Ho Wang Lee's Research Activity about Epidemic Hemorrhagic Fever.
[So] Source:Uisahak;26(1):95-124, 2017 Apr.
[Is] ISSN:1225-505X
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:In the 1960-70s, South Korea was still in the position of a science latecomer. Although the scientific research environment in South Korea at that time was insufficient, there was a scientist who achieved outcomes that could be recognized internationally while acting in South Korea. He was Ho Wang Lee(1928~ ) who found Hantann Virus that causes epidemic hemorrhagic fever for the first time in the world. It became a clue to identify causative viruses of hemorrhagic diseases that were scattered here and there throughout the world. In addition, these outcomes put Ho Wang Lee on the global center of research into epidemic hemorrhagic fever. This paper examines how a Korean scientist who was in the periphery of virology could go into the central area of virology. Also this article shows the process through which the virus found by Ho Wang Lee was registered with the international academia and he proceeded with follow-up research based on this progress to reach the level at which he generalized epidemic hemorrhagic fever related studies throughout the world. While he was conducting the studies, experimental methods that he had never experienced encountered him as new difficulties. He tried to solve the new difficulties faced in his changed status through devices of cooperation and connection. Ho Wang Lee's growth as a researcher can be seen as well as a view of a researcher that grew from a regional level to an international level and could advance from the area of non-mainstream into the mainstream. This analytic tool is meaningful in that it can be another method of examining the growth process of scientists in South Korea or developing countries.
[Mh] Termos MeSH primário: Vírus Hantaan/fisiologia
Febre Hemorrágica com Síndrome Renal/história
Virologia/história
[Mh] Termos MeSH secundário: Febre Hemorrágica com Síndrome Renal/virologia
História do Século XX
História do Século XXI
República da Coreia
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Ps] Nome de pessoa como assunto:Lee HW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:QIS
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.13081/kjmh.2017.26.95


  10 / 5135 MEDLINE  
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[PMID]:28785814
[Au] Autor:Nasukawa T; Uchiyama J; Taharaguchi S; Ota S; Ujihara T; Matsuzaki S; Murakami H; Mizukami K; Sakaguchi M
[Ad] Endereço:School of Veterinary Medicine, Azabu University, Fuchinobe 1-7-71, Chuo-ku, Sagamihara-shi, Kanagawa, 252-0206, Japan.
[Ti] Título:Virus purification by CsCl density gradient using general centrifugation.
[So] Source:Arch Virol;162(11):3523-3528, 2017 Nov.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Virus purification by cesium chloride (CsCl) density gradient, which generally requires an expensive ultracentrifuge, is an essential technique in virology. Here, we optimized virus purification by CsCl density gradient using general centrifugation (40,000 × g, 2 h, 4 °C), which showed almost the same purification ability as conventional CsCl density gradient ultracentrifugation (100,000 × g, 1 h, 4 °C) using phages S13' and φEF24C. Moreover, adenovirus strain JM1/1 was also successfully purified by this method. We suggest that general centrifugation can become a less costly alternative to ultracentrifugation for virus purification by CsCl densiy gradient and will thus encourage research in virology.
[Mh] Termos MeSH primário: Bacteriófagos/classificação
Bacteriófagos/fisiologia
Centrifugação com Gradiente de Concentração/métodos
Césio/química
Cloretos/química
Virologia/métodos
[Mh] Termos MeSH secundário: Centrifugação com Gradiente de Concentração/instrumentação
Virologia/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chlorides); 1KSV9V4Y4I (Cesium); GNR9HML8BA (cesium chloride)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170809
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3513-z



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