| [PMID]: | 28403897 |
| [Au] Autor: | Mossaad E; Salim B; Suganuma K; Musinguzi P; Hassan MA; Elamin EA; Mohammed GE; Bakhiet AO; Xuan X; Satti RA; Inoue N |
| [Ad] Endereço: | Department of Pathology, Parasitology and Microbiology, College of Veterinary Medicine, Sudan University of Science and Technology, P.O. Box 204, Khartoum, Sudan. ehabmssd7@gmail.com. |
| [Ti] Título: | Trypanosoma vivax is the second leading cause of camel trypanosomosis in Sudan after Trypanosoma evansi. |
| [So] Source: | Parasit Vectors;10(1):176, 2017 Apr 13. |
| [Is] ISSN: | 1756-3305 |
| [Cp] País de publicação: | England |
| [La] Idioma: | eng |
| [Ab] Resumo: | BACKGROUND: This study was conducted in response to recurring reports from eastern Sudan of camel trypanosomosis that can no longer be treated by currently available trypanocidal drugs. One hundred and eighty-nine blood samples were obtained from camels in different herds and local markets in the western part of Sudan, and a cross-sectional study was carried out between December 2015 and February 2016 to identify the causative agents and possible circulating genotypes. RESULTS: The prevalence of trypanosomes detected using the conventional parasitological techniques of Giemsa-stained blood smears, wet blood smears and the microhematocrit centrifugation technique (MHCT) was 7% (13/189), 11% (21/189) and 19% (36/189), respectively. However, a multi-species KIN-PCR targeting the ITS region revealed that the prevalence of Trypanosoma evansi was 37% (70/189), while that of T. vivax was 25% (47/189). Consequently, we used a T. evansi-specific PCR (RoTat1.2 VSG gene) to analyse the KIN-PCR-positive samples and a T. vivax-specific PCR (Cathepsin L-like gene) to analyse all of the samples. The prevalence of T. evansi was 59% (41/70), while the prevalence of T. vivax was 31% (59/189). Mixed infections were detected in 18% (34/189) of the samples. These results were further confirmed by sequencing and a phylogenetic analysis of the complete internal transcribed spacer (ITS) region of T. evansi and the TviCatL gene of T. vivax. CONCLUSION: We conclude that T. vivax was newly introduced to the camel population and that T. evansi is no longer the single cause of camel trypanosomosis in Sudan. The presence of T. vivax in camels detected in this study is a challenge in the choice of diagnostic approaches, particularly serology, and PCRs. However, an analysis of drug resistance should be performed, and the genotypic variation should be verified. To our knowledge, this is the first molecular study on T. vivax and mixed-infection with T. vivax and T. evansi in Sudanese camels. |
| [Mh] Termos MeSH primário: |
Camelus/parasitologia
Trypanosoma/classificação
Trypanosoma/isolamento & purificação
Tripanossomíase Africana/veterinária
|
| [Mh] Termos MeSH secundário: |
Animais
Técnicas de Laboratório Clínico/métodos
Análise por Conglomerados
Estudos Transversais
DNA de Protozoário/química
DNA de Protozoário/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Parasitologia/métodos
Filogenia
Reação em Cadeia da Polimerase
Prevalência
Análise de Sequência de DNA
Sudão/epidemiologia
Trypanosoma/genética
Tripanossomíase Africana/epidemiologia
Tripanossomíase Africana/parasitologia
|
| [Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (DNA, Protozoan); 0 (DNA, Ribosomal Spacer) |
| [Em] Mês de entrada: | 1704 |
| [Cu] Atualização por classe: | 170425 |
[Lr] Data última revisão:
| 170425 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 170414 |
| [St] Status: | MEDLINE |
| [do] DOI: | 10.1186/s13071-017-2117-5 |
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