Base de dados : MEDLINE
Pesquisa : H01.158.273.688 [Categoria DeCS]
Referências encontradas : 4479 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 448 ir para página                         

  1 / 4479 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29240337
[Au] Autor:Kerttula AM; Lavikainen A
[Ti] Título:Nucleic acid diagnostic approaches in parasitology.
[So] Source:Duodecim;133(8):742-8, 2017.
[Is] ISSN:0012-7183
[Cp] País de publicação:Finland
[La] Idioma:eng
[Ab] Resumo:Nucleic acid diagnostic technologies are partly replacing traditional microscopy and antigen detection methods in parasitological diagnostics. In particular, the diagnostics of parasitic diarrhea is undergoing a transformation due to the application of polymerase chain reaction (PCR) tests. Diagnostics of malaria is still based on microscopy, but rapid nucleic acid tests are emerging. Laboratories of clinical microbiology in Finland currently provide PCR tests e.g. for intestinal protozoa, Toxoplasma and Trichomonas. Nucleic acid diagnostic methods are superior in specificity and sensitivity, but may give false positive results after a treated infection.
[Mh] Termos MeSH primário: Técnicas de Amplificação de Ácido Nucleico/métodos
Parasitologia/métodos
[Mh] Termos MeSH secundário: Finlândia
Seres Humanos
Malária/diagnóstico
Reação em Cadeia da Polimerase
Toxoplasmose/diagnóstico
Tricomoníase/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE


  2 / 4479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28822208
[Au] Autor:Kiewra D; Rydzanicz K; Czulowska A
[Ad] Endereço:Department of Microbial Ecology and Environmental Protection, Institute of Genetics and Microbiology, University of Wroclaw, ul. Przybyszewskiego 63/77, 51-148 Wroclaw, Poland
[Ti] Título:Professor Elzbieta Lonc, D. Sc. (1951­2017) ­ pro memoriam
[So] Source:Ann Parasitol;63(2):143-146, 2017.
[Is] ISSN:2299-0631
[Cp] País de publicação:Poland
[La] Idioma:eng
[Mh] Termos MeSH primário: Microbiologia
Parasitologia
[Mh] Termos MeSH secundário: História do Século XX
História do Século XXI
Microbiologia/história
Parasitologia/história
Polônia
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Ps] Nome de pessoa como assunto:Lonc E
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170820
[St] Status:MEDLINE
[do] DOI:10.17420/ap6302.99


  3 / 4479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28665738
[Au] Autor:Barrios MA; Kenyon A; Beckstead R
[Ad] Endereço:Department of Poultry Science, The University of Georgia, Athens, GA 30602.
[Ti] Título:Development of a Dry Medium for Isolation of Histomonas meleagridis in the Field.
[So] Source:Avian Dis;61(2):242-244, 2017 Jun.
[Is] ISSN:1938-4351
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Blackhead disease is caused by Histomonas meleagridis, an anaerobic protozoan parasite, and results in mortality rates of up to 100% in turkeys and 30% in chickens. Outbreaks of blackhead disease are unpredictable, and the harvesting of H. meleagridis strains from the field would be a great resource for researchers to study its epidemiology. Therefore, the objective of this study was to develop a dry medium that would allow storage at ambient temperatures until needed. Fifty milliliters of horse serum was dried and then mixed with dry medium M199 with Hanks balanced salts (10.6 g), sodium bicarbonate (0.35 g), and rice powder (0.8 g). To test the ability of reconstituted medium to support growth of H. meleagridis, groups of 10 flasks containing 0.2 g of dry medium were stored for 24 hr at 25 and 60 C before testing. Other groups of flasks containing dry medium were stored at 25, 37, and 42 C for 1, 3, or 6 mo. At each test period, the flasks were reconstituted with 10 ml of water, inoculated with 100 000 H. meleagridis cells, and incubated at 40 C for 48 hr. Fresh liquid medium was used as a control. There were no differences in cell counts in medium stored at 25 or 60 C for 24 hr. After 1 mo, cell counts in reconstituted medium were about half that of fresh liquid medium after 48 hr of incubation. But after 3 and 6 mo, the cell counts were not significantly different in all groups (P < 0.05) after 72 hr of incubation. These results show that dried Dwyer medium can be stored at ambient temperatures for extended times and would be an effective tool for obtaining isolates of H. meleagridis from the field.
[Mh] Termos MeSH primário: Meios de Cultura/química
Parasitologia/métodos
Doenças das Aves Domésticas/parasitologia
Infecções Protozoárias em Animais/parasitologia
Trichomonadida/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Meios de Cultura/metabolismo
Trichomonadida/genética
Trichomonadida/isolamento & purificação
Trichomonadida/metabolismo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1637/11530-110816-ResNote.1


  4 / 4479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28617281
[Au] Autor:Gyawali P; Sidhu JPS; Ahmed W; Jagals P; Toze S
[Ad] Endereço:School of Public Health, The University of Queensland, Herston Road, Herston, Qld 4006, Australia E-mail: pradep033@yahoo.com.
[Ti] Título:Comparison of culture-based, vital stain and PMA-qPCR methods for the quantitative detection of viable hookworm ova.
[So] Source:Water Sci Technol;75(11-12):2615-2621, 2017 Jun.
[Is] ISSN:0273-1223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Accurate quantitative measurement of viable hookworm ova from environmental samples is the key to controlling hookworm re-infections in the endemic regions. In this study, the accuracy of three quantitative detection methods [culture-based, vital stain and propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR)] was evaluated by enumerating 1,000 ± 50 Ancylostoma caninum ova in the laboratory. The culture-based method was able to quantify an average of 397 ± 59 viable hookworm ova. Similarly, vital stain and PMA-qPCR methods quantified 644 ± 87 and 587 ± 91 viable ova, respectively. The numbers of viable ova estimated by the culture-based method were significantly (P < 0.05) lower than vital stain and PMA-qPCR methods. Therefore, both PMA-qPCR and vital stain methods appear to be suitable for the quantitative detection of viable hookworm ova. However, PMA-qPCR would be preferable over the vital stain method in scenarios where ova speciation is needed.
[Mh] Termos MeSH primário: Ancylostoma/isolamento & purificação
Azidas/metabolismo
Azul de Metileno/química
Parasitologia/métodos
Propídio/análogos & derivados
Reação em Cadeia da Polimerase em Tempo Real/métodos
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Animais
Óvulo
Parasitologia/instrumentação
Propídio/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azides); 0 (propidium monoazide); 36015-30-2 (Propidium); T42P99266K (Methylene Blue)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.2166/wst.2017.142


  5 / 4479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28506043
[Au] Autor:Makki M; Dupouy-Camet J; Sajjadi SMS; Naddaf SR; Mobedi I; Rezaeian M; Mohebali M; Mowlavi G
[Ad] Endereço:Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
[Ti] Título:First Paleoparasitological Report on the Animal Feces of Bronze Age Excavated from Shahr-e Sukhteh, Iran.
[So] Source:Korean J Parasitol;55(2):197-201, 2017 Apr.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Shahr-e Sukhteh (meaning burnt city in Persian) in Iran is an archeological site dated back to around 3,200-1,800 BC. It is located in Sistan and Baluchistan Province of Iran and known as the junction of Bronze Age trade routes crossing the Iranian plateau. It was appointed as current study area for paleoparasitological investigations. Excavations at this site have revealed various archeological materials since 1967. In the present study, sheep and carnivore coprolites excavated from this site were analyzed by means of rehydration technique using TSP solution for finding helminth eggs. sp., and sp. eggs were identified, while some other objects similar to Anoplocephalidae and spp. eggs were also retrieved from the samples but their measured parameters did not match those of these species. The present paper illustrates the first paleoparasitological findings of Bronze Age in eastern Iran supporting the economic activities, peopling, and communication as well as the appropriate condition for zoonotic helminthiasis life cycle in Shahr-e Sukhteh archeological site.
[Mh] Termos MeSH primário: Carnívoros/parasitologia
Fezes/parasitologia
Helmintos/isolamento & purificação
Paleontologia
Contagem de Ovos de Parasitas
Parasitologia
Ovinos/parasitologia
[Mh] Termos MeSH secundário: Animais
Helmintos/crescimento & desenvolvimento
História Antiga
Irã (Geográfico)
Estágios do Ciclo de Vida
Paleontologia/métodos
Parasitologia/métodos
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2017.55.2.197


  6 / 4479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28486652
[Au] Autor:Eads DA
[Ad] Endereço:Department of Biology, Colorado State University, Fort Collins, CO 80523.
[Ti] Título:Swabbing Prairie Dog Burrows for Fleas That Transmit Yersinia pestis: Influences on Efficiency.
[So] Source:J Med Entomol;54(5):1273-1277, 2017 Sep 01.
[Is] ISSN:1938-2928
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Scientists and health-care professionals sometimes use a swabbing technique to collect fleas from rodent burrows, and later test the fleas for Yersinia pestis, the causative agent of plague. Detection of Y. pestis is enhanced when large pools of fleas are available. The following study investigated factors that might affect the rate at which fleas are collected from burrows in colonies of black-tailed prairie dogs (Cynomys ludovicianus). Data were collected from 13 colonies in New Mexico during 0600-1000 hours, June-August 2010-2011. Fleas were scarce on swabs inserted into burrows that were not actively used by prairie dogs; fleas are presumably suppressed in burrows that are void of hosts and might have begun to collapse due to a lack of maintenance. Fleas were scarce on swabs inserted into burrows with little sunlight entering the tunnel; many species of fleas use changes in light intensity to locate objects, but if light is limited, it might be difficult to locate a swab. Fleas were scarce on swabs inserted to shallow depths underground, especially during hot mornings, and during the hottest portions of mornings; when conditions are hot above ground, ectothermic fleas might migrate into the deep components of burrows, or become less willing to jump onto hosts, making it difficult to collect the fleas with swabs. If the swabbing technique is used to survey for Y. pestis on colonies of black-tailed prairie dogs, investigators might use the results of this study to modify their methods and increase the number of fleas collected.
[Mh] Termos MeSH primário: Infestações por Pulgas/veterinária
Parasitologia/métodos
Peste/veterinária
Doenças dos Roedores/transmissão
Sciuridae
Sifonápteros/microbiologia
Yersinia pestis/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Meio Ambiente
Infestações por Pulgas/parasitologia
Infestações por Pulgas/transmissão
New Mexico
Peste/microbiologia
Peste/transmissão
Doenças dos Roedores/microbiologia
Doenças dos Roedores/parasitologia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE
[do] DOI:10.1093/jme/tjx090


  7 / 4479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28483382
[Au] Autor:Rinaldo A; Bertuzzo E; Blokesch M; Mari L; Gatto M
[Ad] Endereço:Laboratory of Ecohydrology, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland; Dipartimento ICEA, Università di Padova, Padova, Italy. Electronic address: andrea.rinaldo@epfl.ch.
[Ti] Título:Modeling Key Drivers of Cholera Transmission Dynamics Provides New Perspectives for Parasitology.
[So] Source:Trends Parasitol;33(8):587-599, 2017 08.
[Is] ISSN:1471-5007
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hydroclimatological and anthropogenic factors are key drivers of waterborne disease transmission. Information on human settlements and host mobility on waterways along which pathogens and hosts disperse, and relevant hydroclimatological processes, can be acquired remotely and included in spatially explicit mathematical models of disease transmission. In the case of epidemic cholera, such models allowed the description of complex disease patterns and provided insight into the course of ongoing epidemics. The inclusion of spatial information in models of disease transmission can aid in emergency management and the assessment of alternative interventions. Here, we review the study of drivers of transmission via spatially explicit approaches and argue that, because many parasitic waterborne diseases share the same drivers as cholera, similar principles may apply.
[Mh] Termos MeSH primário: Cólera/transmissão
Modelos Teóricos
Parasitologia
[Mh] Termos MeSH secundário: Cólera/epidemiologia
Água Doce
Seres Humanos
Parasitologia/tendências
Doenças Transmitidas pela Água/epidemiologia
Doenças Transmitidas pela Água/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE


  8 / 4479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28438422
[Au] Autor:Kofer J; Hofer H; Hartmann S
[Ad] Endereço:Institute of Immunology, Center of Infection Medicine, Freie Universität Berlin, Robert-von-Ostertag Strasse 7-13, 14163 Berlin, Germany; Center for Infection Biology and Immunity (ZIBI), Faculty for Veterinary Medicine, Freie Universität Berlin.
[Ti] Título:Next-Generation Parasitologists: Structured Training Programs Meet Educational Challenges.
[So] Source:Trends Parasitol;33(6):423-425, 2017 06.
[Is] ISSN:1471-5007
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Parasite infections are diverse, complex and widespread, and they represent major health threats to people and animals alike. Topics such as vaccine development, drug resistance, immune regulation, vector-borne parasitic diseases, and wildlife parasitology are key issues. Here, we discuss the need and direction of structured educational programs for graduates in modern parasitology.
[Mh] Termos MeSH primário: Educação/normas
Parasitologia/educação
[Mh] Termos MeSH secundário: Currículo/normas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE


  9 / 4479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28420302
[Au] Autor:Platt TR
[Ad] Endereço:Department of Biology, Saint Mary's College, Notre Dame, Indiana 46556. Correspondence should be sent to: tplatt@saintmarys.edu.
[Ti] Título:The Genus Spirorchis MacCallum, 1918 (Digenea: Schistosomatoidea) and the Early History of Parasitology in the United States.
[So] Source:J Parasitol;103(4):407-420, 2017 Aug.
[Is] ISSN:1937-2345
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We know little about the founders of our discipline apart from their scientific contributions and brief biographical sketches, most frequently in published obituaries. A number of years ago, Ralph Lichtenfels, then Director of the National Parasite Collection, sent me photocopies of letters between Henry Baldwin Ward, Horace W. Stunkard, George A. MacCallum, and William G. MacCallum dating from the early years of the 20th century that hinted at a series of conflicts centered on the proposal of Spirorchis MacCallum, 1918 (Digenea: Schistosomatoidea). The description of a fluke that matured in the blood of a tetrapod and that was morphologically similar to the schistosomes of humans was in its time a transformative discovery; and the scientist who published it would have garnered some scholarly recognition. Herein, I provide an historical account of the issues and the motives of each individual and the eventual resolution of these matters.
[Mh] Termos MeSH primário: Parasitologia
[Mh] Termos MeSH secundário: Animais
Correspondência como Assunto
História do Século XX
Ontário
Parasitologia/história
Terminologia como Assunto
Trematódeos/classificação
Infecções por Trematódeos/história
Infecções por Trematódeos/parasitologia
Infecções por Trematódeos/veterinária
Tartarugas/parasitologia
Estados Unidos
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Ps] Nome de pessoa como assunto:Ward HB; MacCallum GA; MacCallum WG; Stunkard HW
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1645/17-15


  10 / 4479 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28403897
[Au] Autor:Mossaad E; Salim B; Suganuma K; Musinguzi P; Hassan MA; Elamin EA; Mohammed GE; Bakhiet AO; Xuan X; Satti RA; Inoue N
[Ad] Endereço:Department of Pathology, Parasitology and Microbiology, College of Veterinary Medicine, Sudan University of Science and Technology, P.O. Box 204, Khartoum, Sudan. ehabmssd7@gmail.com.
[Ti] Título:Trypanosoma vivax is the second leading cause of camel trypanosomosis in Sudan after Trypanosoma evansi.
[So] Source:Parasit Vectors;10(1):176, 2017 Apr 13.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: This study was conducted in response to recurring reports from eastern Sudan of camel trypanosomosis that can no longer be treated by currently available trypanocidal drugs. One hundred and eighty-nine blood samples were obtained from camels in different herds and local markets in the western part of Sudan, and a cross-sectional study was carried out between December 2015 and February 2016 to identify the causative agents and possible circulating genotypes. RESULTS: The prevalence of trypanosomes detected using the conventional parasitological techniques of Giemsa-stained blood smears, wet blood smears and the microhematocrit centrifugation technique (MHCT) was 7% (13/189), 11% (21/189) and 19% (36/189), respectively. However, a multi-species KIN-PCR targeting the ITS region revealed that the prevalence of Trypanosoma evansi was 37% (70/189), while that of T. vivax was 25% (47/189). Consequently, we used a T. evansi-specific PCR (RoTat1.2 VSG gene) to analyse the KIN-PCR-positive samples and a T. vivax-specific PCR (Cathepsin L-like gene) to analyse all of the samples. The prevalence of T. evansi was 59% (41/70), while the prevalence of T. vivax was 31% (59/189). Mixed infections were detected in 18% (34/189) of the samples. These results were further confirmed by sequencing and a phylogenetic analysis of the complete internal transcribed spacer (ITS) region of T. evansi and the TviCatL gene of T. vivax. CONCLUSION: We conclude that T. vivax was newly introduced to the camel population and that T. evansi is no longer the single cause of camel trypanosomosis in Sudan. The presence of T. vivax in camels detected in this study is a challenge in the choice of diagnostic approaches, particularly serology, and PCRs. However, an analysis of drug resistance should be performed, and the genotypic variation should be verified. To our knowledge, this is the first molecular study on T. vivax and mixed-infection with T. vivax and T. evansi in Sudanese camels.
[Mh] Termos MeSH primário: Camelus/parasitologia
Trypanosoma/classificação
Trypanosoma/isolamento & purificação
Tripanossomíase Africana/veterinária
[Mh] Termos MeSH secundário: Animais
Técnicas de Laboratório Clínico/métodos
Análise por Conglomerados
Estudos Transversais
DNA de Protozoário/química
DNA de Protozoário/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Parasitologia/métodos
Filogenia
Reação em Cadeia da Polimerase
Prevalência
Análise de Sequência de DNA
Sudão/epidemiologia
Trypanosoma/genética
Tripanossomíase Africana/epidemiologia
Tripanossomíase Africana/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2117-5



página 1 de 448 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde