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[PMID]: 29422098 [Au] Autor: Huyben D; Boqvist S; Passoth V; Renström L; Allard Bengtsson U; Andréoletti O; Kiessling A; Lundh T; Vågsholm I [Ad] Endereço: Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, 75007, Uppsala, Sweden. david.huyben@slu.se. [Ti] Título: Screening of intact yeasts and cell extracts to reduce Scrapie prions during biotransformation of food waste. [So] Source: Acta Vet Scand;60(1):9, 2018 Feb 08. [Is] ISSN: 1751-0147 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Yeasts can be used to convert organic food wastes to protein-rich animal feed in order to recapture nutrients. However, the reuse of animal-derived waste poses a risk for the transmission of infectious prions that can cause neurodegeneration and fatality in humans and animals. The aim of this study was to investigate the ability of yeasts to reduce prion activity during the biotransformation of waste substrates-thereby becoming a biosafety hurdle in such a circular food system. During pre-screening, 30 yeast isolates were spiked with Classical Scrapie prions and incubated for 72 h in casein substrate, as a waste substitute. Based on reduced Scrapie seeding activity, waste biotransformation and protease activities, intact cells and cell extracts of 10 yeasts were further tested. Prion analysis showed that five yeast species reduced Scrapie seeding activity by approximately 1 log10 or 90%. Cryptococcus laurentii showed the most potential to reduce prion activity since both intact and extracted cells reduced Scrapie by 1 log10 and achieved the highest protease activity. These results show that select forms of yeast can act as a prion hurdle during the biotransformation of waste. However, the limited ability of yeasts to reduce prion activity warrants caution as a sole barrier to transmission as higher log reductions are needed before using waste-cultured yeast in circular food systems. [Mh] Termos MeSH primário: Biotransformação Príons/metabolismo Scrapie/prevenção & controle Gerenciamento de Resíduos/métodos Leveduras/metabolismo [Mh] Termos MeSH secundário: Animais Extratos Celulares/análise Alimentos Parasitologia de Alimentos/normas Parasitologia de Alimentos/tendências Peptídeo Hidrolases/metabolismo Gerenciamento de Resíduos/normas Leveduras/enzimologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cell Extracts); 0 (Prions); EC 3.4.- (Peptide Hydrolases) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180227 [Lr] Data última revisão: 180227 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180210 [St] Status: MEDLINE [do] DOI: 10.1186/s13028-018-0363-y

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[PMID]: 28910293 [Au] Autor: Hoffmann S; Devleesschauwer B; Aspinall W; Cooke R; Corrigan T; Havelaar A; Angulo F; Gibb H; Kirk M; Lake R; Speybroeck N; Torgerson P; Hald T [Ad] Endereço: U.S. Dept. of Agriculture, Economic Research Service, Washington D.C., United States of America. [Ti] Título: Attribution of global foodborne disease to specific foods: Findings from a World Health Organization structured expert elicitation. [So] Source: PLoS One;12(9):e0183641, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: Recently the World Health Organization, Foodborne Disease Burden Epidemiology Reference Group (FERG) estimated that 31 foodborne diseases (FBDs) resulted in over 600 million illnesses and 420,000 deaths worldwide in 2010. Knowing the relative role importance of different foods as exposure routes for key hazards is critical to preventing illness. This study reports the findings of a structured expert elicitation providing globally comparable food source attribution estimates for 11 major FBDs in each of 14 world subregions. METHODS AND FINDINGS: We used Cooke's Classical Model to elicit and aggregate judgments of 73 international experts. Judgments were elicited from each expert individually and aggregated using both equal and performance weights. Performance weighted results are reported as they increased the informativeness of estimates, while retaining accuracy. We report measures of central tendency and uncertainty bounds on food source attribution estimate. For some pathogens we see relatively consistent food source attribution estimates across subregions of the world; for others there is substantial regional variation. For example, for non-typhoidal salmonellosis, pork was of minor importance compared to eggs and poultry meat in the American and African subregions, whereas in the European and Western Pacific subregions the importance of these three food sources were quite similar. Our regional results broadly agree with estimates from earlier European and North American food source attribution research. As in prior food source attribution research, we find relatively wide uncertainty bounds around our median estimates. CONCLUSIONS: We present the first worldwide estimates of the proportion of specific foodborne diseases attributable to specific food exposure routes. While we find substantial uncertainty around central tendency estimates, we believe these estimates provide the best currently available basis on which to link FBDs and specific foods in many parts of the world, providing guidance for policy actions to control FBDs. [Mh] Termos MeSH primário: Doenças Transmitidas por Alimentos/epidemiologia Doenças Transmitidas por Alimentos/mortalidade [Mh] Termos MeSH secundário: Animais Microbiologia de Alimentos Parasitologia de Alimentos Saúde Global Seres Humanos Prevalência Organização Mundial da Saúde [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171012 [Lr] Data última revisão: 171012 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170915 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0183641

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[PMID]: 28767982 [Au] Autor: Noya BA; Pérez-Chacón G; Díaz-Bello Z; Dickson S; Muñoz-Calderón A; Hernández C; Pérez Y; Mauriello L; Moronta E [Ad] Endereço: Universidad Central de Venezuela, Facultad de Medicina, Instituto de Medicina Tropical, Sección de Inmunología, Caracas, Venezuela. [Ti] Título: Description of an oral Chagas disease outbreak in Venezuela, including a vertically transmitted case. [So] Source: Mem Inst Oswaldo Cruz;112(8):569-571, 2017 Aug. [Is] ISSN: 1678-8060 [Cp] País de publicação: Brazil [La] Idioma: eng [Ab] Resumo: We describe the eleventh major outbreak of foodborne Trypanosoma cruzi transmission in urban Venezuela, including evidence for vertical transmission from the index case to her fetus. After confirming fetal death at 24 weeks of gestation, pregnancy interruption was performed. On direct examination of the amniotic fluid, trypomastigotes were detected. T. cruzi specific-polymerase chain reaction (PCR) also proved positive when examining autopsied fetal organs. Finally, microscopic fetal heart examination revealed amastigote nests. Acute orally transmitted Chagas disease can be life threatening or even fatal for pregnant women and unborn fetuses owing to vertical transmission. There is therefore an urgent need to improve national epidemiologic control measures. [Mh] Termos MeSH primário: Doença de Chagas/transmissão Morte Fetal/etiologia Parasitologia de Alimentos Doenças Transmitidas por Alimentos/parasitologia Transmissão Vertical de Doença Infecciosa [Mh] Termos MeSH secundário: Adolescente Adulto Doença de Chagas/complicações Doença de Chagas/epidemiologia Surtos de Doenças Feminino Seres Humanos Hidropisia Fetal/parasitologia Reação em Cadeia da Polimerase Gravidez População Urbana Venezuela/epidemiologia Adulto Jovem [Pt] Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171004 [Lr] Data última revisão: 171004 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170803 [St] Status: MEDLINE

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[PMID]: 28722600 [Au] Autor: Nawa Y; Yoshikawa M; Sawanyawisuth K; Chotmongkol V; Figueiras SF; Benavides M; Diaz Camacho SP [Ad] Endereço: Faculty of Medicine, Tropical Diseases Research Centre, Khon Kaen University, Khon Kaen, Thailand. [Ti] Título: Ocular Gnathostomiasis-Update of Earlier Survey. [So] Source: Am J Trop Med Hyg;97(4):1232-1234, 2017 Oct. [Is] ISSN: 1476-1645 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Ocular gnathostomiasis is rather a rare food-borne zoonosis caused by infection with the larvae of several species of genus and is a representative ocular larva migrans syndrome. In our previous literature survey, we found 73 cases of ocular gnathostomiasis reported up to and including 2009, though additional sporadic cases have been reported in Asia and the Americas since that report. Here, we review 10 additional cases reported since 2010, and also update current findings regarding epidemiological and clinical features in affected patients. [Mh] Termos MeSH primário: Oftalmopatias/parasitologia Gnatostomíase/epidemiologia Gnatostomíase/parasitologia [Mh] Termos MeSH secundário: Adolescente Adulto Animais Feminino Parasitologia de Alimentos Saúde Global Gnatostomíase/diagnóstico Seres Humanos Masculino Meia-Idade Adulto Jovem Zoonoses [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171030 [Lr] Data última revisão: 171030 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 170720 [St] Status: MEDLINE [do] DOI: 10.4269/ajtmh.17-0133

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[PMID]: 28683055 [Au] Autor: Springer YP; Casillas S; Helfrich K; Mocan D; Smith M; Arriaga G; Mixson L; Castrodale L; McLaughlin J [Ti] Título: Two Outbreaks of Trichinellosis Linked to Consumption of Walrus Meat - Alaska, 2016-2017. [So] Source: MMWR Morb Mortal Wkly Rep;66(26):692-696, 2017 Jul 07. [Is] ISSN: 1545-861X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: During 1975-2012, CDC surveillance identified 1,680 trichinellosis cases in the United States with implicated food items; among these cases, 1,219 were attributed to consumption of raw or pork products, and 461 were attributed to nonpork products. Although trichinellosis in the United States has historically been associated with consumption of pork, multiple nonporcine species of wild game also are competent hosts for Trichinella spp. and have been collectively implicated in the majority of trichinellosis cases since the late 1990s (1-4) (Figure 1). During July 2016-May 2017, the Alaska Division of Public Health (ADPH) investigated two outbreaks of trichinellosis in the Norton Sound region associated with consumption of raw or undercooked walrus (Odobenus rosmarus) meat; five cases were identified in each of the two outbreaks. These were the first multiple-case outbreaks of walrus-associated trichinellosis in Alaska since 1992 (Figure 2). Health care providers should inquire about consumption of commercially prepared and personally harvested meats when evaluating suspected trichinellosis cases, especially in areas where consumption of wild game is commonplace. [Mh] Termos MeSH primário: Surtos de Doenças Parasitologia de Alimentos Carne/parasitologia Triquinelose/epidemiologia [Mh] Termos MeSH secundário: Adolescente Adulto Alaska/epidemiologia Animais Feminino Seres Humanos Masculino Trichinella/isolamento & purificação Triquinelose/diagnóstico Morsas [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170713 [Lr] Data última revisão: 170713 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170707 [St] Status: MEDLINE [do] DOI: 10.15585/mmwr.mm6626a3

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[PMID]: 28650216 [Au] Autor: Gracenea M; Gállego L [Ad] Endereço: Laboratory of Parasitology, Department of Biology, Healthcare and the Environment, Faculty of Pharmacy and Food Sciences, University of Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain. [Ti] Título: Brachylaimiasis: Brachylaima spp. (Digenea: Brachylaimidae) Metacercariae Parasitizing the Edible Snail Cornu aspersum (Helicidae) in Spanish Public Marketplaces and Health-Associated Risk Factors. [So] Source: J Parasitol;103(5):440-450, 2017 Oct. [Is] ISSN: 1937-2345 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The edible land snail Cornu aspersum (Pulmonata: Stylommatophora) acts as a second intermediate host in the terrestrial life cycle of Brachylaima spp. trematodes, harboring unencysted metacercariae in its kidney. The ingestion of undercooked infected snails by humans may allow metacercariae to potentially develop to adult stage in the intestine, causing brachylaimiasis, as already seen in Australia. The prevalence and dynamics of C. aspersum parasitization by Brachylaima spp. metacercariae in specimens intended for human consumption in Spanish marketplaces were studied. In total, 3,710 C. aspersum specimens were analyzed over 5 yr, which were obtained from public marketplaces in the Spanish cities of Barcelona, Bilbao, Madrid, Tudela, Valencia, and Zaragoza. The overall prevalence was 41.97% (95% CI: 40.38-45.56%). The Tudela marketplace had the highest values for both the seasonal prevalence and abundance in all studies during autumn (93.57% and 3.09, respectively). This market also gave the highest individual metacercarial burden recorded, 212 metacercariae in a single specimen. Overall, the highest prevalence of Brachylaima spp. occurred in autumn (58.65%) and the lowest in winter (22.64%). There was a seasonal effect on prevalence, which increased from summer to autumn and then decreased in winter. In total, 96 experimental Brachylaima adults were obtained from the metacercariae parasitizing the analyzed snails. These were identified through morphometric tools (principal component analysis) as Brachylaima mascomai (56 in Barcelona, 1 in Bilbao, 7 in Tudela, and 3 in Valencia), and Brachylaima llobregatensis (17 in Barcelona, 8 in Bilbao, 1 in Valencia, and 3 in Zaragoza). Logistic regression modeling, conducted to predict the probability of purchasing parasitized snails using city and season as predictors showed a correct prediction overall of 79.0%, with a significant (p = 0.001) risk effect in the Barcelona-autumn interaction (2.551-38.442), a significant (p = 0.049) protection effect in the Tudela-spring interaction (0.076-0.997), a significant (p < 0.001) risk effect in the Tudela-autumn interaction (4.330-78.584), and a significant (p = 0.014) protection effect in the Valencia-spring interaction (0.033-0.687). The high overall prevalence of Brachylaima spp. metacercariae should be a matter of concern for public health authorities, mainly in countries where C. aspersum is consumed. [Mh] Termos MeSH primário: Parasitologia de Alimentos Caramujos/parasitologia Trematódeos/fisiologia Infecções por Trematódeos/transmissão [Mh] Termos MeSH secundário: Animais Intervalos de Confiança Reservatórios de Doenças/parasitologia Feminino Seres Humanos Estágios do Ciclo de Vida Modelos Logísticos Metacercárias/fisiologia Camundongos Camundongos Endogâmicos ICR Prevalência Análise de Componente Principal Fatores de Risco Estações do Ano Espanha Trematódeos/crescimento & desenvolvimento Infecções por Trematódeos/epidemiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171025 [Lr] Data última revisão: 171025 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170627 [St] Status: MEDLINE [do] DOI: 10.1645/17-29

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[PMID]: 28437182 [Au] Autor: Cong W; Chi WB; Sun WW; Shan XF; Kang YH; Meng QF; Qian AD [Ad] Endereço: 1 College of Marine Science, Shandong University at Weihai , Weihai, Shandong Province, People's Republic of China . [Ti] Título: First Report of Toxoplasma gondii Infection in Common Quails (Coturnix coturnix) Intended for Human Consumption in Three Provinces of Northeastern China. [So] Source: Vector Borne Zoonotic Dis;17(5):351-353, 2017 May. [Is] ISSN: 1557-7759 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Common quails are nutritious food and are also used as medicine in some parts of the world. Until now, no information on Toxoplasma gondii infection in common quails intended for human consumption in China is available. Therefore, this study was carried out to determine the prevalence of T. gondii infection in common quails in six cities from Shandong, Liaoning, and Jilin provinces, China. Out of 620 common quails examined, 59 (9.52%) were seropositive for T. gondii by the modified agglutination test. T. gondii infection in male and female common quails was 8.10% and 10.71%, respectively. The highest prevalence (13.10%) was detected in a group of common quails from Rizhao of Shandong province, followed by common quails from Yantai of Shandong province (13.04%) and the lowest prevalence (5.56%) was detected in a group of common quails from Shenyang of Liaoning province. The results of this survey revealed the presence of T. gondii infection in common quails in China, indicating that common quails are a potential source for transmission of T. gondii to humans, therefore, posing a potential public health concern. To our knowledge, this is the first report of T. gondii infection in common quails in China. [Mh] Termos MeSH primário: Coturnix Doenças das Aves Domésticas/parasitologia Toxoplasma Toxoplasmose Animal/epidemiologia [Mh] Termos MeSH secundário: Animais China/epidemiologia Parasitologia de Alimentos Doenças das Aves Domésticas/sangue Doenças das Aves Domésticas/epidemiologia Toxoplasmose Animal/sangue [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170915 [Lr] Data última revisão: 170915 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170425 [St] Status: MEDLINE [do] DOI: 10.1089/vbz.2016.2078

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[PMID]: 28426643 [Au] Autor: Marder EP; Cieslak PR; Cronquist AB; Dunn J; Lathrop S; Rabatsky-Ehr T; Ryan P; Smith K; Tobin-D'Angelo M; Vugia DJ; Zansky S; Holt KG; Wolpert BJ; Lynch M; Tauxe R; Geissler AL [Ti] Título: Incidence and Trends of Infections with Pathogens Transmitted Commonly Through Food and the Effect of Increasing Use of Culture-Independent Diagnostic Tests on Surveillance - Foodborne Diseases Active Surveillance Network, 10 U.S. Sites, 2013-2016. [So] Source: MMWR Morb Mortal Wkly Rep;66(15):397-403, 2017 Apr 21. [Is] ISSN: 1545-861X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Foodborne diseases represent a substantial public health concern in the United States. CDC's Foodborne Diseases Active Surveillance Network (FoodNet) monitors cases reported from 10 U.S. sites* of laboratory-diagnosed infections caused by nine enteric pathogens commonly transmitted through food. This report describes preliminary surveillance data for 2016 on the nine pathogens and changes in incidences compared with 2013-2015. In 2016, FoodNet identified 24,029 infections, 5,512 hospitalizations, and 98 deaths caused by these pathogens. The use of culture-independent diagnostic tests (CIDTs) by clinical laboratories to detect enteric pathogens has been steadily increasing since FoodNet began surveying clinical laboratories in 2010 (1). CIDTs complicate the interpretation of FoodNet surveillance data because pathogen detection could be affected by changes in health care provider behaviors or laboratory testing practices (2). Health care providers might be more likely to order CIDTs because these tests are quicker and easier to use than traditional culture methods, a circumstance that could increase pathogen detection (3). Similarly, pathogen detection could also be increasing as clinical laboratories adopt DNA-based syndromic panels, which include pathogens not often included in routine stool culture (4,5). In addition, CIDTs do not yield isolates, which public health officials rely on to distinguish pathogen subtypes, determine antimicrobial resistance, monitor trends, and detect outbreaks. To obtain isolates for infections identified by CIDTs, laboratories must perform reflex culture ; if clinical laboratories do not, the burden of culturing falls to state public health laboratories, which might not be able to absorb that burden as the adoption of these tests increases (2). Strategies are needed to preserve access to bacterial isolates for further characterization and to determine the effect of changing trends in testing practices on surveillance. [Mh] Termos MeSH primário: Testes Diagnósticos de Rotina/métodos Testes Diagnósticos de Rotina/utilização Microbiologia de Alimentos Parasitologia de Alimentos Doenças Transmitidas por Alimentos/diagnóstico Doenças Transmitidas por Alimentos/epidemiologia Vigilância da População [Mh] Termos MeSH secundário: Técnicas de Cultura/utilização Seres Humanos Incidência Estados Unidos/epidemiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170502 [Lr] Data última revisão: 170502 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170421 [St] Status: MEDLINE [do] DOI: 10.15585/mmwr.mm6615a1

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[PMID]: 28359911 [Au] Autor: Guardone L; Nucera D; Pergola V; Costanzo F; Costa E; Tinacci L; Guidi A; Armani A [Ad] Endereço: FishLab, Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy. [Ti] Título: Visceral larvae as a predictive index of the overall level of fish batch infection in European anchovies (Engraulis encrasicolus): A rapid procedure for Food Business Operators to assess marketability. [So] Source: Int J Food Microbiol;250:12-18, 2017 Jun 05. [Is] ISSN: 1879-3460 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: The European anchovy (Engraulis encrasicolus), one of the most important pelagic fish resources in the Mediterranean Sea, is frequently infected by anisakid larvae. Food Business Operators (FBOs) should use appropriate sampling plans and analytical methods to avoid commercialization of massively infected batches and reduce the risk of transmission of viable zoonotic larvae. In this study, performed at FishLab (Department of Veterinary Sciences of the University of Pisa) during 2016, an official sampling plan was associated with a digestion protocol for the inspection of anchovies. Considering that anisakid larvae are usually located in the fish visceral cavity and in the adjacent muscles (VM), this part was analyzed. In particular, we assessed the reliability of the digestion of a subsample of 150g (±30g) of VM, randomly collected from 29 specimens, in estimating the marketability of the anchovies' batch. Fifty-seven samples of 29 anchovies were collected. Each anchovy was sectioned to separate VM. All the subsamples were digested, and visible larvae counted. A high correlation between the number of larvae in VM regions and in the total batch was observed, indicating a very significant contribution of the VM region on total number of parasites. The Mean Abundance (MA) was used to assess the batch marketability according to a threshold calculated on the basis of the maximum number of nematodes tolerated per sample. Considering that the MA can be calculated only when the number of examined specimens is known, the number of visible Larvae per gram of tissue (LpG) was calculated on 150g (±30g) of VM subsamples. A LpG marketability threshold was calculated dividing the maximum number of tolerated nematodes by the average weight of a sample of 29 anchovies calculated considering data available in literature. To evaluate the diagnostic performance of the LpG threshold, the marketability of 57 batches assessed on the basis of the MA threshold was assumed as the gold standard. The proposed LpG showed very high Specificity and Sensitivity. These findings suggest that the analysis of VM is representative of the overall infestation of the batch, both when considering the absolute number of parasites and the LpG, and may represent a valid alternative to the whole anchovy digestion. In particular, the use of an automated digestive method, coupled with the aforesaid sampling plan, could allow the procedure to be used by FBOs in operational conditions. [Mh] Termos MeSH primário: Anisaquíase/prevenção & controle Anisakis/isolamento & purificação Peixes/parasitologia Parasitologia de Alimentos/métodos Larva/crescimento & desenvolvimento [Mh] Termos MeSH secundário: Animais Anisaquíase/parasitologia Anisaquíase/transmissão Alimentos Manipulação de Alimentos/métodos Mar Mediterrâneo Músculos/parasitologia Reprodutibilidade dos Testes Vísceras/parasitologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170928 [Lr] Data última revisão: 170928 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170401 [St] Status: MEDLINE

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[PMID]: 28343955 [Au] Autor: Felin E; Näreaho A; Fredriksson-Ahomaa M [Ad] Endereço: Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66, Finland. Electronic address: elina.felin@helsinki.fi. [Ti] Título: Comparison of commercial ELISA tests for the detection of Toxoplasma antibodies in the meat juice of naturally infected pigs. [So] Source: Vet Parasitol;238:30-34, 2017 Apr 30. [Is] ISSN: 1873-2550 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Toxoplasmosis is a globally distributed protozoal zoonosis. Pigs are considered an important reservoir of Toxoplasma gondii and pork a major infection source of human toxoplasmosis. ELISA methods are commonly used diagnostic tools for detecting Toxoplasma infections. They are also used for slaughterhouse-based serological monitoring of toxoplasmosis in pigs to identify positive farms. The methods used are non-standardised with varying sensitivity and specificity. In our study, four commercial ELISA tests for the detection of Toxoplasma antibodies in the meat juice of slaughter pigs were compared with a modified agglutination test (MAT) as a reference. The cut-off values of the ELISA tests provided by the manufacturer varied between 0.20 and 0.50, and clearly influenced prevalence. The sensitivity of tests I, II and III varied between 96.4 and 78.6. Sensitivity was unacceptably low (3.6) for test IV (cut-off=0.30). Tests I, II and III had the highest accuracy and the best agreement with the reference test when a cut-off of 0.30 was used. Test II and III showed very good agreement (K=0.92 and 0.84, respectively) with the MAT. A very strong correlation (Pearson correlation >0.89) was observed between the S/P values of tests I, II and III. Our results demonstrate that the test and cut-off value used influence the results of the apparent seroprevalence studies. [Mh] Termos MeSH primário: Anticorpos Antiprotozoários/química Ensaio de Imunoadsorção Enzimática/veterinária Parasitologia de Alimentos Carne Vermelha/parasitologia Doenças dos Suínos/parasitologia [Mh] Termos MeSH secundário: Animais Ensaio de Imunoadsorção Enzimática/métodos Suínos Doenças dos Suínos/diagnóstico [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antibodies, Protozoan) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171030 [Lr] Data última revisão: 171030 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170328 [St] Status: MEDLINE

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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