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[PMID]: 29367478 [Au] Autor: Sun M; Xu X; Zhang Q; Rui X; Wu J; Dong M [Ad] Endereço: College of Food Science and Technology, Nanjing Agricultural University. [Ti] Título: Ultrasonic-assisted Aqueous Extraction and Physicochemical Characterization of Oil from Clanis bilineata. [So] Source: J Oleo Sci;67(2):151-165, 2018 Feb 01. [Is] ISSN: 1347-3352 [Cp] País de publicação: Japan [La] Idioma: eng [Ab] Resumo: Ultrasound-assisted aqueous extraction (UAAE) was used to extract oil from Clanis bilineata (CB), a traditional edible insect that can be reared on a large scale in China, and the physicochemical property and antioxidant capacity of the UAAE-derived oil (UAAEO) were investigated for the first time. UAAE conditions of CB oil was optimized using response surface methodology (RSM) and the highest oil yield (19.47%) was obtained under optimal conditions for ultrasonic power, extraction temperature, extraction time, and ultrasonic interval time at 400 W, 40°C, 50 min, and 2 s, respectively. Compared with Soxhlet extraction-derived oil (SEO), UAAEO had lower acid (AV), peroxide (PV) and p-anisidine values (PAV) as well as higher polyunsaturated fatty acids contents and thermal stability. Furthermore, UAAEO showed stronger antioxidant activities than those of SEO, according to DPPH radical scavenging and ß-carotene bleaching tests. Therefore, UAAE is a promising process for the large-scale production of CB oil and CB has a developing potential as functional oil resource. [Mh] Termos MeSH primário: Fenômenos Químicos Larva/química Lepidópteros/química Extração Líquido-Líquido/métodos Óleos/química Óleos/isolamento & purificação Ultrassom Água [Mh] Termos MeSH secundário: Animais Antioxidantes Ácidos Graxos Insaturados/análise Óleos/farmacologia Temperatura Ambiente [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); 0 (Fatty Acids, Unsaturated); 0 (Oils); 059QF0KO0R (Water) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180309 [Lr] Data última revisão: 180309 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180126 [St] Status: MEDLINE [do] DOI: 10.5650/jos.ess17108

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[PMID]: 29211285 [Au] Autor: Alcalde MP; Bramante CM; Vivan RR; Amorso-Silva PA; Andrade FB; Duarte MAH [Ad] Endereço: Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Dentística, Endodontia e Materiais Odontológicos, Bauru, SP, Brasil. [Ti] Título: Intradentinal antimicrobial action and filling quality promoted by ultrasonic agitation of epoxy resin-based sealer in endodontic obturation. [So] Source: J Appl Oral Sci;25(6):641-649, 2017 Nov-Dec. [Is] ISSN: 1678-7765 [Cp] País de publicação: Brazil [La] Idioma: eng [Ab] Resumo: MATERIAL AND METHODS: The aim of this study was evaluate the influence of ultrasonic activation (UA) of AH Plus to improve canal and isthmus filing, and analyse the antimicrobial effect against Enterococcus faecalis within dentinal tubules. Thirty mesial roots of mandibular first molars were selected and divided into 2 groups (n = 15): with and without UA of the sealer. Then the root canals were filled by using the single cone technique, and the specimens were sectioned at 2, 4 and 6 mm from the apex for stereomicroscope and confocal laser scanner microscopy (CLSM) analysis. In addition, 30 bovine incisors were contaminated with Enterococcus faecalis and divided into 3 groups (n = 10). The specimens were obturated by using the single cone technique with (G1) and without (G2) UA of the sealer and G3 as the control group. All were sectioned into 6 mm-long cylinders and stained with LIVE/DEAD to assess bacterial viability by CLSM. RESULTS: The UA of the sealer significantly reduced the presence of unfilled areas in the canal and isthmus area in all sections (p<0.05), and there was a significant increase in sealer penetration in both canals and isthmuses (p<0.05). As regards gaps, a significant reduction was found at 2 and 6 mm in the isthmus area of the UA group (p<0.05). Moreover, UA of the sealer significantly reduced bacterial viability in the superficial dentine when compared with the other groups (p<0.05). CONCLUSION: Ultrasonic activation of the AH Plus sealer promoted a better quality of root canal filling and increased the intratubular penetration of sealer, especially in the isthmus area. Additionally, ultrasonic activation of the sealer increased the intradentinal antimicrobial action against Enterococcus faecalis, mainly in the superficial dentine of the root canal. [Mh] Termos MeSH primário: Cavidade Pulpar/microbiologia Enterococcus faecalis/efeitos dos fármacos Resinas Epóxi Materiais Restauradores do Canal Radicular/farmacologia Obturação do Canal Radicular/métodos Ultrassom [Mh] Termos MeSH secundário: Seres Humanos Microscopia Confocal [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Epoxy Resins); 0 (Root Canal Filling Materials) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180227 [Lr] Data última revisão: 180227 [Sb] Subgrupo de revista: D; IM [Da] Data de entrada para processamento: 171207 [St] Status: MEDLINE

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[PMID]: 29370210 [Au] Autor: Ojha KS; Burgess CM; Duffy G; Kerry JP; Tiwari BK [Ad] Endereço: Food Chemistry and Technology, Teagasc Food Research Centre, Dublin, Ireland. [Ti] Título: Integrated phenotypic-genotypic approach to understand the influence of ultrasound on metabolic response of Lactobacillus sakei. [So] Source: PLoS One;13(1):e0191053, 2018. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The lethal effects of soundwaves on a range of microorganisms have been known for almost a century whereas, the use of ultrasound to promote or control their activity is much more recent. Moreover, the fundamental molecular mechanism influencing the behaviour of microorganisms subjected to ultrasonic waves is not well established. In this study, we investigated the influence of ultrasonic frequencies of 20, 45, 130 and 950 kHz on growth kinetics of Lactobacillus sakei. A significant increase in the growth rate of L. sakei was observed following ultrasound treatment at 20 kHz despite the treatment yielding a significant reduction of ca. 3 log cfu/mL in cells count. Scanning electron microscopy showed that ultrasound caused significant changes on the cell surface of L. sakei culture with the formation of pores "sonoporation". Phenotypic microarrays showed that all ultrasound treated L. sakei after exposure to various carbon, nitrogen, phosphorus and sulphur sources had significant variations in nutrient utilisation. Integration of this phenotypic data with the genome of L. sakei revealed that various metabolic pathways were being influenced by the ultrasound treatments. Results presented in this study showed that the physiological response of L. sakei in response to US is frequency dependent and that it can influence metabolic pathways. Hence, ultrasound treatments can be employed to modulate microbial activity for specialised applications. [Mh] Termos MeSH primário: Genótipo Lactobacillus sakei/metabolismo Ultrassom [Mh] Termos MeSH secundário: Carbono/metabolismo Genes Bacterianos Lactobacillus sakei/genética Lactobacillus sakei/crescimento & desenvolvimento Lactobacillus sakei/ultraestrutura Microscopia Eletrônica de Varredura Nitrogênio/metabolismo Fósforo/metabolismo Enxofre/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 27YLU75U4W (Phosphorus); 70FD1KFU70 (Sulfur); 7440-44-0 (Carbon); N762921K75 (Nitrogen) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180222 [Lr] Data última revisão: 180222 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180126 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0191053

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[PMID]: 29338016 [Au] Autor: Baghirov H; Snipstad S; Sulheim E; Berg S; Hansen R; Thorsen F; Mørch Y; Davies CL; Åslund AKO [Ad] Endereço: Department of Physics, The Norwegian University of Science and Technology (NTNU), Trondheim, Norway. [Ti] Título: Ultrasound-mediated delivery and distribution of polymeric nanoparticles in the normal brain parenchyma of a metastatic brain tumour model. [So] Source: PLoS One;13(1):e0191102, 2018. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The treatment of brain diseases is hindered by the blood-brain barrier (BBB) preventing most drugs from entering the brain. Focused ultrasound (FUS) with microbubbles can open the BBB safely and reversibly. Systemic drug injection might induce toxicity, but encapsulation into nanoparticles reduces accumulation in normal tissue. Here we used a novel platform based on poly(2-ethyl-butyl cyanoacrylate) nanoparticle-stabilized microbubbles to permeabilize the BBB in a melanoma brain metastasis model. With a dual-frequency ultrasound transducer generating FUS at 1.1 MHz and 7.8 MHz, we opened the BBB using nanoparticle-microbubbles and low-frequency FUS, and applied high-frequency FUS to generate acoustic radiation force and push nanoparticles through the extracellular matrix. Using confocal microscopy and image analysis, we quantified nanoparticle extravasation and distribution in the brain parenchyma. We also evaluated haemorrhage, as well as the expression of P-glycoprotein, a key BBB component. FUS and microbubbles distributed nanoparticles in the brain parenchyma, and the distribution depended on the extent of BBB opening. The results from acoustic radiation force were not conclusive, but in a few animals some effect could be detected. P-glycoprotein was not significantly altered immediately after sonication. In summary, FUS with our nanoparticle-stabilized microbubbles can achieve accumulation and displacement of nanoparticles in the brain parenchyma. [Mh] Termos MeSH primário: Neoplasias Encefálicas/patologia Encéfalo/metabolismo Modelos Animais de Doenças Nanopartículas Metástase Neoplásica Polímeros/administração & dosagem Ultrassom [Mh] Termos MeSH secundário: Animais Barreira Hematoencefálica Neoplasias Encefálicas/metabolismo Sistemas de Liberação de Medicamentos Feminino Seres Humanos Camundongos Camundongos Endogâmicos NOD Camundongos SCID Polímeros/farmacocinética [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Polymers) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180215 [Lr] Data última revisão: 180215 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180117 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0191102

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[PMID]: 29293593 [Au] Autor: Jiang B; Jia P; Zhao W; Wang W [Ad] Endereço: College of Resource and Civil Engineering, Northeastern University, Shenyang, Liaoning Province, China. [Ti] Título: The application of compressive sampling in rapid ultrasonic computerized tomography (UCT) technique of steel tube slab (STS). [So] Source: PLoS One;13(1):e0190281, 2018. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: This paper explores a new method for rapid structural damage inspection of steel tube slab (STS) structures along randomly measured paths based on a combination of compressive sampling (CS) and ultrasonic computerized tomography (UCT). In the measurement stage, using fewer randomly selected paths rather than the whole measurement net is proposed to detect the underlying damage of a concrete-filled steel tube. In the imaging stage, the â„“1-minimization algorithm is employed to recover the information of the microstructures based on the measurement data related to the internal situation of the STS structure. A numerical concrete tube model, with the various level of damage, was studied to demonstrate the performance of the rapid UCT technique. Real-world concrete-filled steel tubes in the Shenyang Metro stations were detected using the proposed UCT technique in a CS framework. Both the numerical and experimental results show the rapid UCT technique has the capability of damage detection in an STS structure with a high level of accuracy and with fewer required measurements, which is more convenient and efficient than the traditional UCT technique. [Mh] Termos MeSH primário: Aço Tomografia Computadorizada por Raios X/instrumentação Ultrassom [Mh] Termos MeSH secundário: Algoritmos Modelos Teóricos Tomografia Computadorizada por Raios X/métodos [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES [Nm] Nome de substância: 12597-69-2 (Steel) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180215 [Lr] Data última revisão: 180215 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180103 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0190281

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[PMID]: 28452520 [Au] Autor: Müller R; Gupta AK; Zhu H; Pannala M; Gillani US; Fu Y; Caspers P; Buck JR [Ad] Endereço: Department of Mechanical Engineering, Virginia Tech, Blacksburg, Virginia 24061, USA. [Ti] Título: Dynamic Substrate for the Physical Encoding of Sensory Information in Bat Biosonar. [So] Source: Phys Rev Lett;118(15):158102, 2017 Apr 14. [Is] ISSN: 1079-7114 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Horseshoe bats have dynamic biosonar systems with interfaces for ultrasonic emission (reception) that change shape while diffracting the outgoing (incoming) sound waves. An information-theoretic analysis based on numerical and physical prototypes shows that these shape changes add sensory information (mutual information between distant shape conformations <20%), increase the number of resolvable directions of sound incidence, and improve the accuracy of direction finding. These results demonstrate that horseshoe bats have a highly effective substrate for dynamic encoding of sensory information. [Mh] Termos MeSH primário: Quirópteros Ecolocação Ultrassom [Mh] Termos MeSH secundário: Animais Modelos Biológicos Localização de Som [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180209 [Lr] Data última revisão: 180209 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170429 [St] Status: MEDLINE [do] DOI: 10.1103/PhysRevLett.118.158102

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[PMID]: 29390675 [Au] Autor: Abi Ghanem M; Dehoux T; Liu L; Le Saux G; Plawinski L; Durrieu MC; Audoin B [Ad] Endereço: University Bordeaux, CNRS, UMR 5295, I2M, F-33400 Talence, France. [Ti] Título: Opto-acoustic microscopy reveals adhesion mechanics of single cells. [So] Source: Rev Sci Instrum;89(1):014901, 2018 Jan. [Is] ISSN: 1089-7623 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Laser-generated GHz-ultrasonic-based technologies have shown the ability to image single cell adhesion and stiffness simultaneously. Using this new modality, we here demonstrate quantitative indicators to investigate contact mechanics and adhesion processes of the cell. We cultured human cells on a rigid substrate, and we used an inverted pulsed opto-acoustic microscope to generate acoustic pulses containing frequencies up to 100 GHz in the substrate. We map the reflection of the acoustic pulses at the cell-substrate interface to obtain images of the acoustic impedance of the cell, Z , as well as of the stiffness of the interface, K, with 1 µm lateral resolution. Our results show that the standard deviation ΔZ reveals differences between different cell types arising from the multiplicity of local conformations within the nucleus. From the distribution of K-values within the nuclear region, we extract a mean interfacial stiffness, K , that quantifies the average contact force in areas of the cell displaying weak bonding. By analogy with classical contact mechanics, we also define the ratio of the real to nominal contact areas, S /S . We show that K can be interpreted as a quantitative indicator of passive contact at metal-cell interfaces, while S /S is sensitive to active adhesive processes in the nuclear region. The ability to separate the contributions of passive and active adhesion processes should allow gaining insight into cell-substrate interactions, with important applications in tissue engineering. [Mh] Termos MeSH primário: Adesão Celular Microscopia Acústica [Mh] Termos MeSH secundário: Seres Humanos Engenharia Tecidual Ultrassom [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180208 [Lr] Data última revisão: 180208 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180203 [St] Status: MEDLINE [do] DOI: 10.1063/1.5019807

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[PMID]: 29390660 [Au] Autor: Gupta S; Bleuel M; Schneider GJ [Ad] Endereço: Department of Chemistry and Department of Physics, Louisiana State University, Baton Rouge, Louisiana 70803, USA. [Ti] Título: A new ultrasonic transducer sample cell for in situ small-angle scattering experiments. [So] Source: Rev Sci Instrum;89(1):015111, 2018 Jan. [Is] ISSN: 1089-7623 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Ultrasound irradiation is a commonly used technique for nondestructive diagnostics or targeted destruction. We report on a new versatile sonication device that fits in a variety of standard sample environments for neutron and X-ray scattering instruments. A piezoelectric transducer permits measuring of the time-dependent response of the sample in situ during or after sonication. We use small-angle neutron scattering (SANS) to demonstrate the effect of a time-dependent perturbation on the structure factor of micelles formed from sodium dodecyl sulfate surfactant molecules. We observe a substantial change in the micellar structure during and after exposure to ultrasonic irradiation. We also observe a time-dependent relaxation to the equilibrium values of the unperturbed system. The strength of the perturbation of the structure factor depends systematically on the duration of sonication. The relaxation behavior can be well reproduced after multiple times of sonication. Accumulation of the recorded intensities of the different sonication cycles improves the signal-to-noise ratio and permits reaching very short relaxation times. In addition, we present SANS data for the micellar form factor on alkyl-poly (ethylene oxide) surfactant molecules irradiated by ultrasound. Due to the flexibility of our new in situ sonication device, different experiments can be performed, e.g., to explore molecular potentials in more detail by introducing a systematic time-dependent perturbation. [Mh] Termos MeSH primário: Espalhamento a Baixo Ângulo Ultrassom [Mh] Termos MeSH secundário: Micelas Polietilenoglicóis Transdutores [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Micelles); 30IQX730WE (Polyethylene Glycols); B697894SGQ (polyethylene glycol 400) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180208 [Lr] Data última revisão: 180208 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180203 [St] Status: MEDLINE [do] DOI: 10.1063/1.5021370

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[PMID]: 29280631 [Au] Autor: Zhang N; Tu Z; Wang H; Liu G; Wang Z; Huang T; Qin X; Xie X; Wang A [Ad] Endereço: State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang, Jiangxi 330047, China. [Ti] Título: Liquid Chromatography High-Resolution Mass Spectrometry Identifies the Glycation Sites of Bovine Serum Albumin Induced by d-Ribose with Ultrasonic Treatment. [So] Source: J Agric Food Chem;66(3):563-570, 2018 Jan 24. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Ultrasonication is an emerging technology applied in food processing and biological experimental pretreatments. Cavitation phenomena induced during ultrasonic treatment can generate localized high temperature and pressure, which can result in glycation reaction between protein and reducing sugars. In this study, the mixture of bovine serum albumin (BSA) and d-ribose was treated under 600 W for different times. Interestingly, a large amount of carbonized black materials appeared after ultrasonication, while the UV absorbance and intrinsic fluorescence spectra reflecting conformational changes were not obvious. Only 12 sites (11 lysines and 1 arginine) of the BSA with ribose under ultrasonic treatment for 35 min were identified through liquid chromatography high-resolution mass spectrometry (LCHR-MS). K547, K548, R359/R360, and K587 were the most reactive glycated sites, with the average degree of substitution per peptide molecule (DSP) value ranging from 15 to 35%. The glycated modification was distributed not only in domain III, but also in domains I and II. The glycated modification could occur during ultrasonic treatment, thereby influencing the properties of biomacromolecule after extraction. [Mh] Termos MeSH primário: Ribose/química Soroalbumina Bovina/química [Mh] Termos MeSH secundário: Motivos de Aminoácidos Animais Cromatografia Líquida Glicosilação Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz Ultrassom [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 27432CM55Q (Serum Albumin, Bovine); 681HV46001 (Ribose) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180207 [Lr] Data última revisão: 180207 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171228 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.7b04578

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[PMID]: 28453970 [Au] Autor: Yamamoto R; Koyama D; Matsukawa M [Ad] Endereço: Faculty of Life and Medical Sciences, Doshisha University, 1-3 Tataramiyakodani, Kyotanabe, Kyoto 610-0321, Japan; Wave Electronics Research Center, Doshisha University, 1-3 Tataramiyakodani, Kyotanabe, Kyoto 610-0321, Japan. [Ti] Título: On-chip ultrasonic manipulation of microparticles by using the flexural vibration of a glass substrate. [So] Source: Ultrasonics;79:81-86, 2017 08. [Is] ISSN: 1874-9968 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: As biotechnology develops, techniques for manipulating and separating small particles such as cells and DNA are required in the life sciences. This paper investigates on-chip manipulation of microparticles in small channels by using ultrasonic vibration. The chip consists of a rectangular glass substrate with a cross-shaped channel (cross-section: 2.0×2.0mm ) and four lead zirconate titanate transducers attached to the substrate's four corners. To efficiently generate the flexural vibration mode on the chip, we used finite element analysis to optimize the configurations of the glass substrate and transducers. Silicon carbide microparticles with an average diameter of 50µm were immersed in the channels, which were filled with ethanol. By applying an in-phase input voltage of 75V at 225kHz to the four transducers, a flexural vibration mode with a wavelength of 13mm was excited on the glass substrate, and this flexural vibration generated an acoustic standing wave in the channel. The particles could be trapped at the nodal lines of the standing wave. By controlling the driving phase difference between the two pairs of transducers, the vibrational distribution of the substrate could be moved along the channels so that the acoustic standing wave moved in the same direction. The trapped particles could be manipulated by the two-phase drive, and the transport direction could be switched at the junction of the channels orthogonally by changing the combination of the driving condition to four transducers. [Mh] Termos MeSH primário: Vidro/química Chumbo/química Titânio/química Ultrassom/métodos Zircônio/química [Mh] Termos MeSH secundário: Análise de Elementos Finitos Tamanho da Partícula Som Vibração [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 12626-81-2 (lead titanate zirconate); 2P299V784P (Lead); C6V6S92N3C (Zirconium); D1JT611TNE (Titanium) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180118 [Lr] Data última revisão: 180118 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170429 [St] Status: MEDLINE

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