Base de dados : MEDLINE
Pesquisa : H01.770.644.578 [Categoria DeCS]
Referências encontradas : 6 [refinar]
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  1 / 6 MEDLINE  
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[PMID]:29317618
[Au] Autor:Kirtane AR; Abouzid O; Minahan D; Bensel T; Hill AL; Selinger C; Bershteyn A; Craig M; Mo SS; Mazdiyasni H; Cleveland C; Rogner J; Lee YL; Booth L; Javid F; Wu SJ; Grant T; Bellinger AM; Nikolic B; Hayward A; Wood L; Eckhoff PA; Nowak MA; Langer R; Traverso G
[Ad] Endereço:Department of Chemical Engineering and David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
[Ti] Título:Development of an oral once-weekly drug delivery system for HIV antiretroviral therapy.
[So] Source:Nat Commun;9(1):2, 2018 01 09.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The efficacy of antiretroviral therapy is significantly compromised by medication non-adherence. Long-acting enteral systems that can ease the burden of daily adherence have not yet been developed. Here we describe an oral dosage form composed of distinct drug-polymer matrices which achieved week-long systemic drug levels of the antiretrovirals dolutegravir, rilpivirine and cabotegravir in a pig. Simulations of viral dynamics and patient adherence patterns indicate that such systems would significantly reduce therapeutic failures and epidemiological modelling suggests that using such an intervention prophylactically could avert hundreds of thousands of new HIV cases. In sum, weekly administration of long-acting antiretrovirals via a novel oral dosage form is a promising intervention to help control the HIV epidemic worldwide.
[Mh] Termos MeSH primário: Fármacos Anti-HIV/administração & dosagem
Sistemas de Liberação de Medicamentos/métodos
Compostos Heterocíclicos com 3 Anéis/administração & dosagem
Piridonas/administração & dosagem
Rilpivirina/administração & dosagem
[Mh] Termos MeSH secundário: Administração Oral
Animais
Fármacos Anti-HIV/farmacocinética
Fármacos Anti-HIV/uso terapêutico
Avaliação Pré-Clínica de Medicamentos
Compostos Heterocíclicos com 3 Anéis/farmacocinética
Compostos Heterocíclicos com 3 Anéis/uso terapêutico
Seres Humanos
Modelos Teóricos
Cooperação do Paciente
Estudo de Prova de Conceito
Piridonas/farmacocinética
Piridonas/uso terapêutico
Rilpivirina/farmacocinética
Rilpivirina/uso terapêutico
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-HIV Agents); 0 (GSK1265744); 0 (Heterocyclic Compounds, 3-Ring); 0 (Pyridones); DKO1W9H7M1 (dolutegravir); FI96A8X663 (Rilpivirine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02294-6


  2 / 6 MEDLINE  
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[PMID]:28467301
[Au] Autor:Wolff JN; Gemmell NJ; Tompkins DM; Dowling DK
[Ad] Endereço:School of Biological Sciences, Monash University, Victoria, Australia.
[Ti] Título:Introduction of a male-harming mitochondrial haplotype via 'Trojan Females' achieves population suppression in fruit flies.
[So] Source:Elife;6, 2017 05 03.
[Is] ISSN:2050-084X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pests are a global threat to biodiversity, ecosystem function, and human health. Pest control approaches are thus numerous, but their implementation costly, damaging to non-target species, and ineffective at low population densities. The Trojan Female Technique (TFT) is a prospective self-perpetuating control technique that is species-specific and predicted to be effective at low densities. The goal of the TFT is to harness naturally occurring mutations in the mitochondrial genome that impair male fertility while having no effect on females. Here, we provide proof-of-concept for the TFT, by showing that introduction of a male fertility-impairing mtDNA haplotype into replicated populations of causes numerical population suppression, with the magnitude of effect positively correlated with its frequency at trial inception. Further development of the TFT could lead to establishing a control strategy that overcomes limitations of conventional approaches, with broad applicability to invertebrate and vertebrate species, to control environmental and economic pests.
[Mh] Termos MeSH primário: DNA Mitocondrial/genética
Haplótipos
Infertilidade Masculina
Controle de Mosquitos/métodos
Mutação
Estudo de Prova de Conceito
[Mh] Termos MeSH secundário: Animais
Drosophila melanogaster
Feminino
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Mitochondrial)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  3 / 6 MEDLINE  
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[PMID]:28455259
[Au] Autor:Wengreen HJ; Nix E; Madden GJ
[Ad] Endereço:Utah State University, 8700 Old Main Hill, Logan, UT 84321, United States. Electronic address: heidi.wengreen@usu.edu.
[Ti] Título:The effect of social norms messaging regarding skin carotenoid concentrations among college students.
[So] Source:Appetite;116:39-44, 2017 09 01.
[Is] ISSN:1095-8304
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Descriptive social-normative messaging positively influences short-term dietary choices and healthy food intake. The purpose of this study was to evaluate the effects of descriptive normative messages on college students' skin carotenoid concentrations (a biomarker of carotenoid-containing fruit and vegetable [FV] intake) over an 8-week period. 251 college students consented to participate and 74% completed the study. Students were randomly assigned to groups who, following a baseline evaluation of skin-carotenoid levels, were told how their score ranked within a peer group of college students attending the same university (Individualized Normative group), that their score was in the lower 20th percentile of the peer group (Manipulated Normative group), or were given no information about their score or the peer group (Control group). Skin carotenoid concentrations were reassessed 8 weeks after the normative messages were presented or withheld. Skin carotenoid levels of those in the Manipulated Normative group increased significantly more than did scores of those in the Control group (t (126) = 3.74, p < 0.001; d = 0.67), but these students' self-reported FV intake did not increase. This finding suggests normative messaging can influence behavior for up to 8 weeks, but future research must better evaluate if the increase in skin carotenoids reflects increased FV consumption, increased consumption of carotenoid-containing FV (with decreased consumption of other FV), or is accounted for by some other behavior change (e.g., increased use of supplements). These findings support further exploration of normative messaging as a technique for producing the long-term behavior change needed to impact public health.
[Mh] Termos MeSH primário: Carotenoides/metabolismo
Frutas
Dieta Saudável
Cooperação do Paciente
Pele/metabolismo
Normas Sociais
Verduras
[Mh] Termos MeSH secundário: Adulto
Biomarcadores/metabolismo
Comportamento de Escolha
Feminino
Preferências Alimentares
Dieta Saudável/psicologia
Seres Humanos
Internet
Masculino
Inquéritos Nutricionais
Ciências da Nutrição/educação
Cooperação do Paciente/psicologia
Estudo de Prova de Conceito
Estudantes
Universidades
Utah
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Biomarkers); 36-88-4 (Carotenoids)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  4 / 6 MEDLINE  
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[PMID]:28456668
[Au] Autor:Sarcey E; Serres A; Tindy F; Chareyre A; Ng S; Nicolas M; Vetter E; Bonnevay T; Abachin E; Mallet L
[Ad] Endereço:Sanofi Pasteur, Analytical Research and Development Department EU, Campus Mérieux-1541, Avenue Marcel Mérieux, 69280, Marcy L'Etoile, France. Electronic address: Eric.Sarcey@sanofi.com.
[Ti] Título:Quantifying low-frequency revertants in oral poliovirus vaccine using next generation sequencing.
[So] Source:J Virol Methods;246:75-80, 2017 Aug.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Spontaneous reversion to neurovirulence of live attenuated oral poliovirus vaccine (OPV) serotype 3 (chiefly involving the n.472U>C mutation), must be monitored during production to ensure vaccine safety and consistency. Mutant analysis by polymerase chain reaction and restriction enzyme cleavage (MAPREC) has long been endorsed by the World Health Organization as the preferred in vitro test for this purpose; however, it requires radiolabeling, which is no longer supported by many laboratories. We evaluated the performance and suitability of next generation sequencing (NGS) as an alternative to MAPREC. The linearity of NGS was demonstrated at revertant concentrations equivalent to the study range of 0.25%-1.5%. NGS repeatability and intermediate precision were comparable across all tested samples, and NGS was highly reproducible, irrespective of sequencing platform or analysis software used. NGS was performed on OPV serotype 3 working seed lots and monovalent bulks (n=21) that were previously tested using MAPREC, and which covered the representative range of vaccine production. Percentages of 472-C revertants identified by NGS and MAPREC were comparable and highly correlated (r≥0.80), with a Pearson correlation coefficient of 0.95585 (p<0.0001). NGS demonstrated statistically equivalent performance to that of MAPREC for quantifying low-frequency OPV serotype 3 revertants, and offers a valid alternative to MAPREC.
[Mh] Termos MeSH primário: Sequenciamento de Nucleotídeos em Larga Escala/métodos
Mutação
Vacina Antipólio Oral/genética
Poliovirus/isolamento & purificação
Poliovirus/patogenicidade
[Mh] Termos MeSH secundário: Seres Humanos
Mutação Puntual
Poliovirus/genética
Reação em Cadeia da Polimerase
Estudo de Prova de Conceito
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Poliovirus Vaccine, Oral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


  5 / 6 MEDLINE  
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[PMID]:28471417
[Au] Autor:Neuhaus J; Schiffer E; Mannello F; Horn LC; Ganzer R; Stolzenburg JU
[Ad] Endereço:Department of Urology, Research Laboratory, University of Leipzig, Liebigstraße 19, 04103 Leipzig, Germany. jochen.neuhaus@medizin.uni-leipzig.de.
[Ti] Título:Protease Expression Levels in Prostate Cancer Tissue Can Explain Prostate Cancer-Associated Seminal Biomarkers-An Explorative Concept Study.
[So] Source:Int J Mol Sci;18(5), 2017 May 04.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Previously, we described prostate cancer (PCa) detection (83% sensitivity; 67% specificity) in seminal plasma by CE-MS/MS. Moreover, advanced disease was distinguished from organ-confined tumors with 80% sensitivity and 82% specificity. The discovered biomarkers were naturally occurring fragments of larger seminal proteins, predominantly semenogelin 1 and 2, representing endpoints of the ejaculate liquefaction. Here we identified proteases putatively involved in PCa specific protein cleavage, and examined gene expression and tissue protein levels, jointly with cell localization in normal prostate (nP), benign prostate hyperplasia (BPH), seminal vesicles and PCa using qPCR, Western blotting and confocal laser scanning microscopy. We found differential gene expression of chymase (CMA1), matrix metalloproteinases (MMP3, MMP7), and upregulation of MMP14 and tissue inhibitors (TIMP1 and TIMP2) in BPH. In contrast tissue protein levels of MMP14 were downregulated in PCa. MMP3/TIMP1 and MMP7/TIMP1 ratios were decreased in BPH. In seminal vesicles, we found low-level expression of most proteases and, interestingly, we also detected TIMP1 and low levels of TIMP2. We conclude that MMP3 and MMP7 activity is different in PCa compared to BPH due to fine regulation by their inhibitor TIMP1. Our findings support the concept of seminal plasma biomarkers as non-invasive tool for PCa detection and risk stratification.
[Mh] Termos MeSH primário: Biomarcadores/metabolismo
Metaloproteinases da Matriz/metabolismo
Neoplasias da Próstata/metabolismo
Sêmen/enzimologia
[Mh] Termos MeSH secundário: Idoso
Estudos de Casos e Controles
Quimases/genética
Quimases/metabolismo
Seres Humanos
Masculino
Metaloproteinases da Matriz/genética
Meia-Idade
Estudo de Prova de Conceito
Neoplasias da Próstata/patologia
Inibidor Tecidual de Metaloproteinase-1/genética
Inibidor Tecidual de Metaloproteinase-1/metabolismo
Inibidor Tecidual de Metaloproteinase-2/genética
Inibidor Tecidual de Metaloproteinase-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (TIMP1 protein, human); 0 (TIMP2 protein, human); 0 (Tissue Inhibitor of Metalloproteinase-1); 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2); EC 3.4.21.39 (Chymases); EC 3.4.24.- (Matrix Metalloproteinases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE


  6 / 6 MEDLINE  
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[PMID]:28459074
[Au] Autor:Rosenthal KS; Stone S; Koski G; Zimmerman DH
[Ad] Endereço:College of Medicine, Roseman University of Health Sciences, 10530 Discovery Drive, Las Vegas, NV 89135, USA.
[Ti] Título:LEAPS Vaccine Incorporating HER-2/neu Epitope Elicits Protection That Prevents and Limits Tumor Growth and Spread of Breast Cancer in a Mouse Model.
[So] Source:J Immunol Res;2017:3613505, 2017.
[Is] ISSN:2314-7156
[Cp] País de publicação:Egypt
[La] Idioma:eng
[Ab] Resumo:The prototype J-LEAPS T cell vaccine for HER-2/neu breast cancer (J-HER) consists of the murine HER-2/neu H-2 CD8 T cell epitope covalently attached through a triglycine linker to the J-immune cell binding ligand (ICBL) (human 2 microglobulin peptide). The J-ICBL was chosen for its potential to promote Th1/Tc1 responses. In this proof-of-concept study, the ability of J-HER to prevent or treat cancer was tested in the TUBO cell-challenged BALB/c mouse model for HER-2/neu-expressing tumors. The J-HER vaccine was administered as an emulsion in Montanide ISA-51 without the need for a more potent adjuvant. When administered as a prophylactic vaccination before tumor challenge, J-HER protected against tumor development for at least 48 days. Despite eliciting protection, antibody production in J-HER-immunized, TUBO-challenged mice was less than that in unimmunized mice. More importantly, therapeutic administration of J-HER one week after challenge with TUBO breast cancer cells limited the spread of the tumors and the morbidity and the mortality in the challenged mice. The ability to elicit responses that prevent spread of the TUBO tumor by J-HER suggests its utility as a neoimmunoadjuvant therapy to surgery. Individual or mixtures of J-LEAPS vaccines can be readily prepared to include different CD8 T cell epitopes to optimize tumor therapy and customize treatment for individuals with different HLA types.
[Mh] Termos MeSH primário: Vacinas Anticâncer
Neoplasias Mamárias Experimentais/prevenção & controle
Neoplasias Mamárias Experimentais/terapia
[Mh] Termos MeSH secundário: Animais
Neoplasias da Mama/genética
Neoplasias da Mama/imunologia
Neoplasias da Mama/prevenção & controle
Neoplasias da Mama/terapia
Linfócitos T CD8-Positivos/imunologia
Vacinas Anticâncer/genética
Vacinas Anticâncer/imunologia
Vacinas Anticâncer/uso terapêutico
Linhagem Celular Tumoral
Modelos Animais de Doenças
Progressão da Doença
Epitopos de Linfócito T/imunologia
Feminino
Genes erbB-2
Imunoglobulina G/sangue
Neoplasias Mamárias Experimentais/imunologia
Neoplasias Mamárias Experimentais/patologia
Camundongos
Camundongos Endogâmicos BALB C
Metástase Neoplásica/prevenção & controle
Estudo de Prova de Conceito
Linfócitos T Citotóxicos/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cancer Vaccines); 0 (Epitopes, T-Lymphocyte); 0 (Immunoglobulin G)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1155/2017/3613505



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