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  1 / 19414 MEDLINE  
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[PMID]:29231025
[Au] Autor:Shen X; Li M; Wang YL; Chen YL; Lin Y; Zhao ZM; Que TZ
[Ad] Endereço:Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China.
[Ti] Título:[Comparison of MPure-12 Automatic Nucleic Acid Purification and Chelex-100 Method].
[So] Source:Fa Yi Xue Za Zhi;33(2):168-170, 2017 Apr.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVES: To explore the forensic application value of MPure-12 automatic nucleic acid purification (MPure-12 Method) for DNA extraction by extracting and typing DNA from bloodstains and various kinds of biological samples with different DNA contents. METHODS: Nine types of biological samples, such as bloodstains, semen stains, and saliva were collected. DNA were extracted using MPure-12 method and Chelex-100 method, followed by PCR amplification and electrophoresis for obtaining STR-profiles. RESULTS: The samples such as hair root, chutty, butt, muscular tissue, saliva stain, bloodstain and semen stain were typed successfully by MPure-12 method. Partial alleles were lacked in the samples of saliva, and the genotyping of contact swabs was unsatisfactory. Additional, all of the bloodstains (20 µL, 15 µL, 10 µL, 5 µL, 1 µL) showed good typing results using Chelex-100 method. But the loss of alleles occurred in 1 µL blood volume by MPure-12 method. CONCLUSIONS: MPure-12 method is suitable for DNA extraction of a certain concentration blood samples.Chelex-100 method may be better for the extraction of trace blood samples.This instrument used in nucleic acid extraction has the advantages of simplicity of operator, rapidity, high extraction efficiency, high rate of reportable STR-profiles and lower man-made pollution.
[Mh] Termos MeSH primário: Quelantes
DNA/isolamento & purificação
Medicina Legal/métodos
Reação em Cadeia da Polimerase/métodos
Poliestirenos
Polivinil
[Mh] Termos MeSH secundário: Alelos
Manchas de Sangue
DNA/sangue
Impressões Digitais de DNA
Genótipo
Seres Humanos
Masculino
Resinas Sintéticas
Saliva
Sêmen/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chelating Agents); 0 (Polystyrenes); 0 (Polyvinyls); 0 (Resins, Synthetic); 11139-85-8 (Chelex 100); 80208-96-4 (chelex); 9007-49-2 (DNA)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2017.02.013


  2 / 19414 MEDLINE  
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[PMID]:29205002
[Au] Autor:Ding DX; Ding M
[Ad] Endereço:Department of Forensic Serology, Faculty of Forensic Medicine, China Medical University, Shenyang 110001, China.
[Ti] Título:[Application of Multiple Displacement Amplification in Samples with Inhibitors].
[So] Source:Fa Yi Xue Za Zhi;32(5):342-345, 2016 Oct.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVES: To explore the ability of inhibition resistibility of multiple displacement amplification (MDA) in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods (MBM) to purify sample. METHODS: Different concentrations of hemoglobin and humid acid (HA) mixed with DNA samples and then divided the samples into MDA group, MBM group and control group. locus was amplified and detected by polyacrylamide gel electrophoresis detection system and AmpFâ„“STR® Identifiler™ Plus Kit-capillary electrophoresis detection system. RESULTS: When hemoglobin concentrations exceed 1 ng/µL or HA concentrations exceed 0.1 ng/µL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/µL or HA concentrations exceed 1 ng/µL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors. CONCLUSIONS: MDA has the capability to remove the inhibition of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.
[Mh] Termos MeSH primário: DNA/análise
Medicina Legal/métodos
Técnicas de Amplificação de Ácido Nucleico
[Mh] Termos MeSH secundário: Eletroforese em Gel de Poliacrilamida
Hemoglobinas
Seres Humanos
Substâncias Húmicas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hemoglobins); 0 (Humic Substances); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2016.05.006


  3 / 19414 MEDLINE  
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[PMID]:28457516
[Au] Autor:Nakata M; Kasuda S; Yuui K; Kudo R; Hatake K
[Ad] Endereço:Department of Legal Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan. Electronic address: dc112064@naramed-u.ac.jp.
[Ti] Título:Relevance of hemolysis-induced tissue factor expression on monocytes in soft clot formation in alcohol-containing blood.
[So] Source:Leg Med (Tokyo);25:83-88, 2017 Mar.
[Is] ISSN:1873-4162
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:The fluidity of cadaveric blood is an important characteristic in the post-mortem examination of cases of asphyxial death. Although it is empirically known that soft blood clots are present in cadaveric blood containing alcohol, the relationship between such clots and blood alcohol is unclear. We addressed this issue through in vitro studies using blood collected from healthy volunteers. Assessment of global hemostasis by rotational thromboelastometry revealed that ethanol treatment enhanced the procoagulant activity of whole blood. However, ethanol inhibited epinephrine-induced platelet aggregation, whereas plasma levels of von Willebrand factor and the activity of coagulation factors VIII and IX were unaffected. In contrast, tissue factor (TF) activity was higher in plasma obtained from ethanol-treated whole blood than that in plasma from untreated blood. Ethanol induced hemolysis of red blood cells, and the consequent hemoglobin (Hb) release promoted de novo synthesis of TF in isolated monocytes, as determined by real-time reverse transcription PCR, western blotting, and flow cytometry. However, ethanol itself did not induce TF expression in monocytes. Given that TF activates the extrinsic coagulation pathway and amplifies hemostatic reactions, Hb-induced TF expression in monocytes might contribute to soft blood clot formation.
[Mh] Termos MeSH primário: Coagulação Sanguínea/efeitos dos fármacos
Etanol/sangue
Hemólise
Monócitos/efeitos dos fármacos
Tromboplastina/efeitos dos fármacos
[Mh] Termos MeSH secundário: Autopsia
Cadáver
Citometria de Fluxo
Medicina Legal
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
3K9958V90M (Ethanol); 9035-58-9 (Thromboplastin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  4 / 19414 MEDLINE  
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[PMID]:28457515
[Au] Autor:Nishi E; Watanabe K; Tashiro Y; Sakai K
[Ad] Endereço:Forensic Science Division, Department of Criminal Investigation, Oita Prefectural Police HQ, 3-1-1 Otemachi, Oita 870-8502, Japan; Laboratory of Soil and Environmental Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate Schoo
[Ti] Título:Terminal restriction fragment length polymorphism profiling of bacterial flora derived from single human hair shafts can discriminate individuals.
[So] Source:Leg Med (Tokyo);25:75-82, 2017 Mar.
[Is] ISSN:1873-4162
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Human hairs are the trace evidence most commonly encountered at many crime scenes. However, they have not been effectively utilized for actual criminal investigations because of the low accuracy of their morphological inspection, low detection rate of short tandem repeat (STR) typing, and the problem of heteroplasmy in mitochondrial DNA analysis. Here, we examined the possibility of individual discrimination by comparing profiles of bacterial flora on hair. We carried out the profiling of terminal restriction fragment length polymorphisms (T-RFLP) of the amplified bacterial 16S ribosomal RNA (rRNA) gene from hair samples. Compared with existing STR typing methods that use hair roots, this method using hair shafts allowed the detection of stable bacterial DNA. We successfully obtained the T-RFLP profile from single hair shafts of all volunteers tested. The profiles were specific to each individual, and multiple profiles obtained from the individual him/herself showed higher similarity than those from different individuals. These individual-specific profiles were stably obtained from samples from most volunteers, when collected again after 6months. Storage of the collected hair samples at -30°C was effective for obtaining reproducible T-RF profiles. When unidentified hair samples collected in the laboratory were compared with a pre-constructed database, 17 of 22 hairs were assigned to a small group of people, including the corresponding individuals. These results show that T-RFLP analysis of bacterial flora on a hair shaft found at a crime scene could provide useful information for narrowing down a suspect.
[Mh] Termos MeSH primário: Bactérias/genética
Cabelo/fisiologia
Polimorfismo de Fragmento de Restrição/genética
[Mh] Termos MeSH secundário: Feminino
Medicina Legal
Seres Humanos
Masculino
RNA Ribossômico 16S/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  5 / 19414 MEDLINE  
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[PMID]:28457514
[Au] Autor:Ueki M; Takeshita H; Fujihara J; Kimura-Kataoka K; Iida R; Yasuda T
[Ad] Endereço:Department of Medical Genetics and Biochemistry, School of Medical Sciences, University of Fukui, Fukui 910-1193, Japan.
[Ti] Título:Simple screening method for copy number variations associated with physical features.
[So] Source:Leg Med (Tokyo);25:71-74, 2017 Mar.
[Is] ISSN:1873-4162
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Recent studies of copy number variations (CNVs) associated with physical features, such as body mass index, body height or bone length, have suggested that such CNVs could serve as markers in forensic cases involving unidentified individuals. However, the process of cataloging CNVs has been slow because of the cumbersome nature and low reliability of the procedures involved. Here we describe a simple quantitative real-time PCR (Q-PCR) method for screening of medicolegally useful CNVs, which does not require reference DNA with known copy number. The first step is to prepare a chimeric plasmid vector including one copy each of the single-copy gene-specific sequence as the internal standard, and the target CNV-specific sequence. To assess the validity of this new method, we analyzed CNVs in the LTBP1 and ETV6 gene regions, both of which are candidate CNVs associated with body height. The PCR efficiencies for the single-copy (reference) gene and the target CNV were similar, indicating that quantitation was reliable. Furthermore, simulated analysis of the LTBP1 CNV using mock samples prepared by mixing vectors in varying proportions showed that this analytical method allowed correct determination of the LTBP1 copy number. These results demonstrated that our simple method has considerable potential for screening of trait-related CNVs that would be useful for forensic casework.
[Mh] Termos MeSH primário: Variações do Número de Cópias de DNA/genética
Variações do Número de Cópias de DNA/fisiologia
Medicina Legal
Programas de Rastreamento/métodos
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Feminino
Seres Humanos
Masculino
Meia-Idade
Fenótipo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  6 / 19414 MEDLINE  
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[PMID]:28457507
[Au] Autor:Aramaki T; Ikeda T; Usui A; Funayama M
[Ad] Endereço:Department of Forensic Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan. Electronic address: aramaki@forensic.med.tohoku.ac.jp.
[Ti] Título:Age estimation by ossification of thyroid cartilage of Japanese males using Bayesian analysis of postmortem CT images.
[So] Source:Leg Med (Tokyo);25:29-35, 2017 Mar.
[Is] ISSN:1873-4162
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Many studies have reported that the degree of thyroid cartilage ossification correlates with age. However, its use in forensics has been limited by individual variation, tissue fragility, and observer subjectivity. We examined a new method for age estimation from the degree of ossification of thyroid cartilage using postmortem computed tomography (CT) and Bayesian analysis. The scoring of CT density values, ossification rate, and morphological changes was performed in 131 Japanese males (20-80years old), and an age estimation table was created and verified in 39 other Japanese males. We tried two types of prior probability; one was males undergoing postmortem CT imaging at our institute, and the other was the age composition of the male population in Japan. The differences in percentage in each age group were observed, especially in the ninth decade, but they were not large enough to change the result. Two decedents in their 30s and 80s could not be assigned an age group. Likelihood might have had more significant influence than prior probability. Bayesian approach and new scoring system of thyroid cartilage ossification might be useful as an age estimation method. Although the low posterior probability in decedents the age ≧70 remains a problem, the advantage of Bayesian estimation that it can sequentially update the age estimation table by factoring in exceptional data can solve this problem. In this study, accuracy of age estimation did not dramatically increase, however, Bayesian statistics might become a useful technique for examining any tissue samples subject to age-related calcification.
[Mh] Termos MeSH primário: Determinação da Idade pelo Esqueleto/métodos
Osteogênese
Cartilagem Tireóidea/patologia
Tomografia Computadorizada por Raios X
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Teorema de Bayes
Medicina Legal
Seres Humanos
Japão
Masculino
Meia-Idade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  7 / 19414 MEDLINE  
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[PMID]:29231027
[Au] Autor:Liu F; Dang YH
[Ad] Endereço:Department of Forensic Science, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China.
[Ti] Título:[Research Progress on Forensic Dentistry].
[So] Source:Fa Yi Xue Za Zhi;33(2):175-180, 2017 Apr.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Forensic dentistry is an interdiscipline of forensic medicine and stomatology, which provides legal information by collecting, testing and assessing the dental evidence scientifically. In this review, the present application of forensic dentistry has been described, such as the estimation of age, sex, species, occupation and living habit, as well as the identification of individual, domestic violence or abuse, which aims to enrich and improve forensic dentistry for making it be more useful in forensic medicine even in juridical practice.
[Mh] Termos MeSH primário: Determinação da Idade pelos Dentes
Odontologia Legal/métodos
[Mh] Termos MeSH secundário: Antropologia Forense
Medicina Legal
Seres Humanos
Dente
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2017.02.015


  8 / 19414 MEDLINE  
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[PMID]:29231008
[Au] Autor:Zeng Y; Ma JL; Chen L
[Ad] Endereço:Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
[Ti] Título:[Significance of Hypoxia-related microRNA for Estimating the Cause of Mechanical Asphyxia Death].
[So] Source:Fa Yi Xue Za Zhi;33(1):38-41, 2017 Feb.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Under hypoxia condition, microRNA (miRNA) can interact with transcription factors for regulating the cell metabolism, angiogenesis, erythropoiesis, cellular proliferation, differentiation and apoptosis. The biological processes above may play an important role in mechanical asphyxia death. This article reviews the regulating function of miRNA under hypoxia condition and the influence of hypoxia to biosynthesis of miRNA, which may provide some new ideas to the research of miRNA on determining the cause of mechanical asphyxia death in the field of forensic medicine.
[Mh] Termos MeSH primário: Acidentes
Obstrução das Vias Respiratórias/fisiopatologia
Asfixia/patologia
Hipóxia/genética
MicroRNAs/genética
[Mh] Termos MeSH secundário: Apoptose
Asfixia/mortalidade
Causas de Morte
Morte
Medicina Legal
Seres Humanos
Hipóxia/metabolismo
Hipóxia/fisiopatologia
MicroRNAs/metabolismo
Oxigênio
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (MicroRNAs); S88TT14065 (Oxygen)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2017.01.010


  9 / 19414 MEDLINE  
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[PMID]:29419499
[Au] Autor:Santos W
[Ad] Endereço:Dept. Agrárias e Exatas, Universidade Estadual da Paraíba, Catolé do Rocha, Brazil.
[Ti] Título:Forensic entomology in the veterinary context.
[So] Source:Vet Rec;182(5):137-138, 2018 02 03.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Entomologia
Ciências Forenses
[Mh] Termos MeSH secundário: Animais
Dípteros
Medicina Legal
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180209
[St] Status:MEDLINE
[do] DOI:10.1136/vr.k407


  10 / 19414 MEDLINE  
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[PMID]:29227827
[Au] Autor:Drury A; Cunningham C
[Ad] Endereço:Centre for Anatomy and Human Identification, University of Dundee, Dow Street, Dundee, DD15EH, United Kingdom. Electronic address: anne.drury@medportal.ca.
[Ti] Título:Determining when a fracture occurred: Does the method matter? Analysis of the similarity of three different methods for estimating time since fracture of juvenile long bones.
[So] Source:J Forensic Leg Med;53:97-105, 2018 Jan.
[Is] ISSN:1878-7487
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Radiographic fracture date estimation is a critical component of skeletal trauma analysis in the living. Several timetables have been proposed for how the appearance of radiographic features can be interpreted to provide a likely time frame for fracture occurrence. This study compares three such timetables for pediatric fractures, by Islam et al. (2000), Malone et al. (2011), and Prosser et al. (2012), in order to determine whether the fracture date ranges produced by using these methods are in agreement with one another. Fracture date ranges were estimated for 112 long bone fractures in 96 children aged 1-17 years, using the three different timetables. The extent of similarity of the intervals was tested by statistically comparing the overlap between the ranges. Results showed that none of the methods were in perfect agreement with one another. Differences seen included the size of the estimated date range for when a fracture occurred, and the specific dates given for both the upper and lower ends of the fracture date range. There was greater similarity between the ranges produced by Malone et al. (2011) and both the other two studies than there was between Islam et al. (2000) and Prosser et al. (2012). The greatest similarity existed between Malone et al. (2011) and Islam et al. (2000). The extent of differences between methods can vary widely, depending on the fracture analysed. Using one timetable gives an average earliest possible fracture date of less than 2 days before another, but the range was extreme, with one method estimating minimum time since fracture as 25 days before another method for a given fracture. In most cases, one method gave maximum time since fracture as a week less than the other two methods, but range was extreme and some estimates were nearly two months different. The variability in fracture date estimates given by these timetables indicates that caution should be exercised when estimating the timing of a juvenile fracture if relying solely on one of the published guides. Future research should be undertaken to compare these methods on a population of known fracture timing, and to better understand the relationship between age of the individual, skeletal health, fracture healing rates, and radiographic characteristics of fracture healing.
[Mh] Termos MeSH primário: Consolidação da Fratura
Fraturas Ósseas/diagnóstico por imagem
[Mh] Termos MeSH secundário: Adolescente
Criança
Pré-Escolar
Feminino
Medicina Legal/métodos
Seres Humanos
Lactente
Masculino
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE



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