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[PMID]:27902321
[Au] Autor:Formella M; Gatherer D
[Ad] Endereço:Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster LA1 4YW, UK.
[Ti] Título:The serology of Ebolavirus - a wider geographical range, a wider genus of viruses or a wider range of virulence?
[So] Source:J Gen Virol;97(12):3120-3130, 2016 Dec.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Viruses of the genus Ebolavirus are the causative agents of Ebola virus disease (EVD), of which there have been only 25 recorded outbreaks since the discovery of Zaire and Sudan ebolaviruses in the late 1970s. Until the west African outbreak commencing in late 2013, EVD was confined to an area of central Africa stretching from the coast of Gabon through the Congo river basin and eastward to the Great Lakes. Nevertheless, population serological studies since 1976, most of which were carried out in the first two decades after that date, have suggested a wider distribution and more frequent occurrence across tropical Africa. We review this body of work, discussing the various methods employed over the years and the degree to which they can currently be regarded as reliable. We conclude that there is adequate evidence for a wider geographical range of exposure to Ebolavirus or related filoviruses and discuss three possibilities that could account for this: (a) EVD outbreaks have been misidentified as other diseases in the past; (b) unidentified, and clinically milder, species of the genus Ebolavirus circulate over a wider range than the most pathogenic species; and (c) EVD may be subclinical with a frequency high enough that smaller outbreaks may be unidentified. We conclude that the second option is the most likely and therefore predict the future discovery of other, less virulent, members of the genus Ebolavirus.
[Mh] Termos MeSH primário: Ebolavirus/isolamento & purificação
Doença pelo Vírus Ebola/virologia
[Mh] Termos MeSH secundário: África/epidemiologia
Animais
Anticorpos Antivirais/sangue
Ebolavirus/classificação
Ebolavirus/imunologia
Ebolavirus/patogenicidade
Doença pelo Vírus Ebola/sangue
Doença pelo Vírus Ebola/epidemiologia
Seres Humanos
Sorologia
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000638


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[PMID]:27846275
[Au] Autor:Lindblom A; Wallménius K; Sjöwall J; Fryland L; Wilhelmsson P; Lindgren PE; Forsberg P; Nilsson K
[Ad] Endereço:Unit of Infectious Diseases, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
[Ti] Título:Prevalence of Rickettsia spp. in Ticks and Serological and Clinical Outcomes in Tick-Bitten Individuals in Sweden and on the Åland Islands.
[So] Source:PLoS One;11(11):e0166653, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tick-transmitted diseases are an emerging health problem, and the hard tick Ixodes ricinus is the main vector for Borrelia spp., tick-borne encephalitis virus and most of the spotted fever Rickettsiae in Europe. The aim of the present study was to examine the incidence of rickettsial infection in the southernmost and south central parts of Sweden and the Åland Islands in Finland, the risk of infection in humans and its correlation with a bite of a Rickettsia-infected tick, the self-reported symptoms of rickettsial disease, and the prevalence of co-infection between Rickettsia spp. and Borrelia spp. Persons with a recent tick bite were enrolled through public media and asked to answer a questionnaire, provide a blood sample and bring detached ticks at enlistment and at follow-up three months later. Blood samples were previously analysed for Borrelia spp. antibodies and, for this report, analysed for antibodies to Rickettsia spp. by immunofluorescence and in 16 cases also using Western Blot. Ninety-six (44.0%) of the 218 participants were seropositive for IgG antibodies to Rickettsia spp. Forty (18.3%) of the seropositive participants had increased titres at the follow-up, indicating recent/current infection, while four (1.8%) had titres indicating probable recent/current infection (≥1:256). Of 472 ticks, 39 (8.3%) were Rickettsia sp. positive. Five (31.3%) of 16 participants bitten by a Rickettsia-infected tick seroconverted. Experience of the self-reported symptoms nausea (p = 0.006) and radiating pain (p = 0.041) was more common among those with recent, current or probable infection compared to those who did not seroconvert. Participants who showed seroreactivity or seroconversion to Rickettsia spp. had more symptoms than those who were seronegative. Seven (3.2%) participants showed seroconversion to Borrelia spp., and three (1.4%) of these showed seroconversion to both Rickettsia spp. and Borrelia spp., in accordance with previous studies in Sweden. Symptoms of rickettsial disease were in most of the cases vague and general that were difficult to differentiate from other tick-borne diseases.
[Mh] Termos MeSH primário: Infecções por Borrelia/transmissão
Coinfecção/transmissão
Encefalite Transmitida por Carrapatos/transmissão
Infecções por Rickettsia/transmissão
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Borrelia/isolamento & purificação
Borrelia/patogenicidade
Infecções por Borrelia/epidemiologia
Infecções por Borrelia/microbiologia
Infecções por Borrelia/virologia
Coinfecção/sangue
Coinfecção/microbiologia
Coinfecção/virologia
Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação
Vírus da Encefalite Transmitidos por Carrapatos/patogenicidade
Encefalite Transmitida por Carrapatos/sangue
Encefalite Transmitida por Carrapatos/microbiologia
Encefalite Transmitida por Carrapatos/virologia
Feminino
Febre/sangue
Febre/microbiologia
Febre/fisiopatologia
Febre/virologia
Seres Humanos
Ixodes/patogenicidade
Ixodes/virologia
Masculino
Meia-Idade
Rickettsia/isolamento & purificação
Rickettsia/patogenicidade
Infecções por Rickettsia/sangue
Infecções por Rickettsia/microbiologia
Infecções por Rickettsia/virologia
Sorologia
Suécia
Picadas de Carrapatos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161116
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166653


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[PMID]:27678365
[Au] Autor:Broide E; Matalon S; Kriger-Sharabi O; Richter V; Shirin H; Leshno M
[Ad] Endereço:Efrat Broide, Shay Matalon, Ofra Kriger-Sharabi, Vered Richter, Haim Shirin, The Kamila Gonczarowski Institute of Gastroenterology, Assaf Harofeh Medical Center, Zerifin 70300, Israel.
[Ti] Título:Cost effectiveness of routine duodenal biopsies in iron deficiency anemia.
[So] Source:World J Gastroenterol;22(34):7813-23, 2016 Sep 14.
[Is] ISSN:2219-2840
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AIM: To investigate the cost effectiveness of routine small bowel biopsies (SBBs) in patients with iron deficiency anemia (IDA) independent of their celiac disease (CD) serology test results. METHODS: We used a state transition Markov model. Two strategies were compared: routine SBBs during esophagogastroduodenoscopy (EGD) in all patients with IDA regardless their celiac serology status (strategy A) vs SBBs only in IDA patients with positive serology (strategy B). The main outcomes were quality adjusted life years (QALY), average cost and the incremental cost effectiveness ratio (ICER). One way sensitivity analysis was performed on all variables and two way sensitivity analysis on selected variables were done. In order to validate the results, a Monte Carlo simulation of 100 sample trials with 10, and an acceptability curve were performed. RESULTS: Strategy A of routine SBBs yielded 19.888 QALYs with a cost of $218.10 compared to 19.887 QALYs and $234.17 in strategy B. In terms of cost-effectiveness, strategy A was the dominant strategy, as long as the cost of SBBs stayed less than $67. In addition, the ICER of strategy A was preferable, providing the cost of biopsy stays under $77. Monte Carlo simulation demonstrated that strategy A yielded the same QALY but with lower costs than strategy B. CONCLUSION: Our model suggests that EGD with routine SBBs is a cost-effective approach with improved QALYs in patients with IDA when the prevalence of CD is 5% or greater. SBBs should be a routine screening tool for CD among patients with IDA, regardless of their celiac antibody status.
[Mh] Termos MeSH primário: Anemia Ferropriva/economia
Anemia Ferropriva/patologia
Biópsia/economia
Doença Celíaca/patologia
Análise Custo-Benefício
Procedimentos Cirúrgicos do Sistema Digestório/métodos
Duodeno/cirurgia
[Mh] Termos MeSH secundário: Anemia Ferropriva/complicações
Biópsia/métodos
Doença Celíaca/complicações
Doença Celíaca/diagnóstico
Doença Celíaca/economia
Endoscopia do Sistema Digestório
Medicina Baseada em Evidências
Seres Humanos
Cadeias de Markov
Prevalência
Modelos de Riscos Proporcionais
Anos de Vida Ajustados por Qualidade de Vida
Sorologia/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170517
[Lr] Data última revisão:
170517
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160929
[St] Status:MEDLINE
[do] DOI:10.3748/wjg.v22.i34.7813


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[PMID]:27677396
[Au] Autor:Bazán PC; Lo Presti MS; Strauss M; Báez AL; Miler N; Paglini PA; Rivarola HW
[Ad] Endereço:Centro de estudios e investigación de la enfermedad de Chagas y Leishmaniasis. Cátedra de Física Biomédica, (INICSA), CONICET. Facultad de Ciencias Médicas. Universidad Nacional de Córdoba, Santa Rosa 1085, Córdoba, Argentina. Electronic address: bazancarolina5@gmail.com.
[Ti] Título:Quantitative PCR and unconventional serological methods to evaluate clomipramine treatment effectiveness in experimental Trypanosoma cruzi infection.
[So] Source:Exp Mol Pathol;101(2):274-280, 2016 Oct.
[Is] ISSN:1096-0945
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Clomipramine (CLO), a tricyclic antidepressant drug, has been used for the treatment of mice infected with Trypanosoma cruzi. In this work we evaluated the effectiveness of CLO treatment upon T. cruzi-infected mice in the chronic phase of the experimental infection using Quantitative polymerase chain reaction (qPCR) and recombinant ELISA. Sixty Swiss albino mice were inoculated with 50 trypomastigote forms of T. cruzi (Tulahuen strain). CLO treatment consisted of 5mg/kg/day during 60days by intraperitoneal injection, beginning on day 90 post infection (p.i) when the mice presented electrocardiographic (ECG) alterations compatible with the chronic phase of the disease. The evolution of experimental infection and the treatment efficacy were studied through survival, electrocardiography, serology using a mixture and individual (1, 2, 13, 30, 36 and SAPA) recombinant proteins from epimastigotes and trypomastigotes of T. cruzi; and qPCR on days 180 and 270 p.i. CLO treatment in the chronic phase decreased the parasite load, reduced the levels of antibodies against antigen 13 throughout 270days p.i and reversed the ECG abnormalities in the treated animals, from 100% of the mice with alterations at the beginning of the treatment to only 20% of the mice with alterations by day 270 p.i. This study shows that qPCR and the use of recombinant antigens are more sensitive to evaluate the effectiveness of the treatment and proves that clomipramine may be considered as a new chemotherapy for the chronic phase of the disease.
[Mh] Termos MeSH primário: Doença de Chagas/sangue
Doença de Chagas/tratamento farmacológico
Clomipramina/uso terapêutico
Reação em Cadeia da Polimerase em Tempo Real/métodos
Sorologia/métodos
Trypanosoma cruzi/fisiologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antiprotozoários/sangue
Doença de Chagas/diagnóstico por imagem
Doença de Chagas/parasitologia
DNA de Protozoário/sangue
Eletrocardiografia
Feminino
Masculino
Camundongos
Parasitemia/tratamento farmacológico
Padrões de Referência
Análise de Sobrevida
Resultado do Tratamento
Trypanosoma cruzi/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (DNA, Protozoan); NUV44L116D (Clomipramine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170529
[Lr] Data última revisão:
170529
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160929
[St] Status:MEDLINE


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[PMID]:27438093
[Au] Autor:Panzarin V; Toffan A; Abbadi M; Buratin A; Mancin M; Braaen S; Olsen CM; Bargelloni L; Rimstad E; Cattoli G
[Ad] Endereço:OIE Reference Laboratory for Viral Encephalopathy and Retinopathy, Department of Comparative Biomedical Sciences, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro (PD), Italy.
[Ti] Título:Molecular Basis for Antigenic Diversity of Genus Betanodavirus.
[So] Source:PLoS One;11(7):e0158814, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Betanodaviruses are the causative agents of viral nervous necrosis (VNN), a devastating disease for the Mediterranean mariculture. Four different betanodavirus species are recognized, Striped jack-, Redspotted grouper-, Tiger puffer-, and Barfin flounder nervous necrosis virus (SJNNV, RGNNV, TPNNV and BFNNV), but there is little knowledge on their antigenic properties. In order to describe the serological relationships among different betanodavirus genotypes, serum neutralization assays were performed using rabbit polyclonal antisera against eight fish nodaviruses that cover a wide species-, temporal-, spatial- and genetic range. The results indicate that the SJNNV and RGNNV are antigenically distinct, constituting serotypes A and C, respectively. The TPNNV and BFNNV, the latter representing cold-water betanodaviruses, are antigenically related and cluster within serotype B. The reassortant viruses RGNNV/SJNNV and SJNNV/RGNNV group within serotypes A and C, respectively, indicating that the coat protein encoded by RNA2 acts as major immunoreactivity determinant. Immunostaining of in vitro expressed wild type and chimeric capsid proteins between the RGNNV and the SJNNV species indicated that the C-terminal part of the capsid protein retains the immunoreactive portion. The amino acid (aa) residues determining RGNNV and SJNNV antigenic diversity were mapped to aa residues 217-256 and aa 257-341, respectively. Neutralization of reverse genetics derived chimeric viruses indicated that these areas determine the neutralizing epitopes. The data obtained are crucial for the development of targeted serological tests for the diagnosis of VNN, and informative for development of cross-protective vaccines against various betanodavirus genotypes.
[Mh] Termos MeSH primário: Variação Antigênica/imunologia
Nodaviridae/imunologia
[Mh] Termos MeSH secundário: Animais
Proteínas do Capsídeo/metabolismo
Linhagem Celular
Análise por Conglomerados
Células Epiteliais/metabolismo
Células Epiteliais/virologia
Peixes/virologia
Coloração Negativa
Testes de Neutralização
Nodaviridae/classificação
Nodaviridae/isolamento & purificação
Nodaviridae/ultraestrutura
Filogenia
Análise de Componente Principal
Proteínas Recombinantes/metabolismo
Genética Reversa
Sorologia
Estatísticas não Paramétricas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Recombinant Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0158814


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[PMID]:27130394
[Au] Autor:Özler K; Aktas E; Atay Ç; Yilmaz B; Arikan M; Güngör S
[Ad] Endereço:Dr. Abdurrahman Yurtaslan Oncology Training and Research Hospital, Department of Orthopaedics and Traumatology, Ankara, Turkey.
[Ti] Título:Serum and knee synovial fluid matrix metalloproteinase-13 and tumor necrosis factor-alpha levels in patients with late-stage osteoarthritis.
[So] Source:Acta Orthop Traumatol Turc;50(3):356-61, 2016.
[Is] ISSN:1017-995X
[Cp] País de publicação:Turkey
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The aim of this study was to compare serum and synovial fluid levels of matrix metalloproteinase-13 (MMP-13) and tumor necrosis factor-alpha (TNF-α) in 2 stages of osteoarthritis, and investigate their correlation with Western Ontario and McMaster Universities Arthritis Index (WOMAC) scores. METHODS: Forty-two patients (mean age: 64±8.8 years) with grade 3 and grade 4 knee osteoarthritis according to Kellgren-Lawrence criteria were enrolled in the study and underwent total knee arthroplasty. TNF-α and MMP-13 levels were measured preoperatively from venous blood samples and intraoperatively from knee synovial fluid via enzyme-linked immunosorbent assay. Preoperative and 1-month postoperative knee functions were assessed by WOMAC. RESULTS: Grade 4 synovial fluid MMP-13 (4.76±5.82 pg/ml) was elevated compared to grade 3 (3.95±4.45), whereas grade 3 serum MMP-13 (1.128±0.308 pg/ml) was found elevated compared to grade 4 (1.038±0.204) (p=0.438, p=0.430, respectively). Grade 4 serum TNF-α (0.253±0.277) was elevated compared to grade 3 (0.206±0.219), whereas grade 3 synovial fluid TNF-α (0.129±0.052) was elevated compared to grade 4 (0.118±0.014). Positive correlation was observed between synovial fluid MMP-13 levels and postoperative WOMAC scores. Mean serum TNF-α (0.226±0.246 pg/ml) was elevated compared to synovial levels (0.124±1.59), and synovial MMP-13 (4.31±1.24) was elevated compared to serum levels (1.089±1.519). CONCLUSION: Despite the systemic increase in TNF-α levels concordant with osteoarthritis grade, MMP-13 levels are elevated via local manner, with a significant correlation with WOMAC scores.
[Mh] Termos MeSH primário: Artroplastia do Joelho
Metaloproteinase 13 da Matriz/química
Osteoartrite do Joelho/metabolismo
Líquido Sinovial/química
Fator de Necrose Tumoral alfa/química
[Mh] Termos MeSH secundário: Idoso
Feminino
Seres Humanos
Masculino
Metaloproteinase 13 da Matriz/sangue
Meia-Idade
Sorologia
Fator de Necrose Tumoral alfa/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tumor Necrosis Factor-alpha); EC 3.4.24.- (MMP13 protein, human); EC 3.4.24.- (Matrix Metalloproteinase 13)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170209
[Lr] Data última revisão:
170209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160501
[St] Status:MEDLINE
[do] DOI:10.3944/AOTT.2015.15.0115


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[PMID]:27088660
[Au] Autor:Eisenberg T; Ewers C; Rau J; Akimkin V; Nicklas W
[Ad] Endereço:a Hessisches Landeslabor , Gießen , Germany.
[Ti] Título:Approved and novel strategies in diagnostics of rat bite fever and other Streptobacillus infections in humans and animals.
[So] Source:Virulence;7(6):630-48, 2016 Aug 17.
[Is] ISSN:2150-5608
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rat bite fever (RBF), a worldwide occurring and most likely under-diagnosed zoonosis caused by Streptobacillus moniliformis, represents the most prominent disease of Streptobacillus infections. Recently, novel members have been described, from which a reservoir in rats and other animal species and a zoonotic potential can be assumed. Despite regularly published case reports, diagnostics of RBF continues to represent a 'diagnostic dilemma', because the mostly applied 16S rRNA sequence analysis may be uncertain for proper pathogen identification. Virtually nothing is known regarding prevalence in humans and animal reservoirs. For a realistic assessment of the pathogen's spread, epidemiology and virulence traits, future studies should focus on the genomic background of Streptobacillus. Full genome sequence analyses of a representative collection of strains might facilitate to unequivocally identify and type isolates. Prevalence studies using selective enrichment mechanisms may also enable the isolation of novel strains and candidate species of this neglected group of microorganisms.
[Mh] Termos MeSH primário: Infecções por Fusobacterium/diagnóstico
Técnicas de Diagnóstico Molecular/métodos
Febre por Mordedura de Rato/diagnóstico
Streptobacillus/genética
Streptobacillus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Infecções por Fusobacterium/microbiologia
Genes Essenciais
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Seres Humanos
Testes de Sensibilidade Microbiana
Microscopia Eletrônica
RNA Ribossômico 16S/genética
Febre por Mordedura de Rato/microbiologia
Ratos
Sorologia/métodos
Espectrofotometria Infravermelho
Streptobacillus/efeitos dos fármacos
Streptobacillus/ultraestrutura
Zoonoses/diagnóstico
Zoonoses/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160419
[St] Status:MEDLINE
[do] DOI:10.1080/21505594.2016.1177694


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[PMID]:26824935
[Au] Autor:Verma SK; Minicucci L; Murphy D; Carstensen M; Humpal C; Wolf P; Calero-Bernal R; Cerqueira-Cézar CK; Kwok OC; Su C; Hill D; Dubey JP
[Ad] Endereço:United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland, 20705-2350.
[Ti] Título:Antibody Detection and Molecular Characterization of Toxoplasma gondii from Bobcats (Lynx rufus), Domestic Cats (Felis catus), and Wildlife from Minnesota, USA.
[So] Source:J Eukaryot Microbiol;63(5):567-71, 2016 Sep.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Little is known of the epidemiology of toxoplasmosis in Minnesota. Here, we evaluated Toxoplasma gondii infection in 50 wild bobcats (Lynx rufus) and 75 other animals on/near 10 cattle farms. Antibodies to T. gondii were assayed in serum samples or tissue fluids by the modified agglutination test (MAT, cut-off 1:25). Twenty nine of 50 bobcats and 15 of 41 wildlife trapped on the vicinity of 10 farms and nine of 16 adult domestic cats (Felis catus) and six of 14 domestic dogs resident on farms were seropositive. Toxoplasma gondii oocysts were not found in feces of any felid. Tissues of all seropositive wild animals trapped on the farm were bioassayed in mice and viable T. gondii was isolated from two badgers (Taxidea taxus), two raccoons (Procyon lotor), one coyote (Canis latrans), and one opossum (Didelphis virginiana). All six T. gondii isolates were further propagated in cell culture. Multi-locus PCR-RFLP genotyping using 10 markers (SAG1, SAG2 (5'-3'SAG2, and alt.SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico), and DNA from cell culture derived tachyzoites revealed three genotypes; #5 ToxoDataBase (1 coyote, 1 raccoon), #1 (1 badger, 1 raccoon, 1 opossum), and #2 (1 badger). This is the first report of T. gondii prevalence in domestic cats and in bobcats from Minnesota, and the first isolation of viable T. gondii from badger.
[Mh] Termos MeSH primário: Animais Selvagens/parasitologia
Anticorpos Antiprotozoários/sangue
Gatos/parasitologia
Lynx/parasitologia
Toxoplasma/genética
Toxoplasma/imunologia
Toxoplasma/isolamento & purificação
Toxoplasmose Animal/parasitologia
[Mh] Termos MeSH secundário: Testes de Aglutinação/veterinária
Animais
Animais Domésticos/parasitologia
Bovinos/parasitologia
Coiotes/parasitologia
DNA de Protozoário
Cães/parasitologia
Fezes/parasitologia
Genótipo
Camundongos
Minnesota/epidemiologia
Mustelidae/parasitologia
Oocistos
Gambás/parasitologia
Reação em Cadeia da Polimerase/veterinária
Polimorfismo de Fragmento de Restrição
Prevalência
Guaxinins/parasitologia
Sorologia/métodos
Toxoplasma/classificação
Toxoplasmose Animal/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (DNA, Protozoan)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160130
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12301


  9 / 726 MEDLINE  
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[PMID]:26679224
[Au] Autor:Alidjinou EK; Lazrek M; Libier L; Bocket L; Dewilde A; Engelmann I; Hober D
[Ad] Endereço:Université de Lille, Faculté de Médecine, CHU Lille, Laboratoire de Virologie, EA3610 Lille, France.
[Ti] Título:A patient with fever, abdominal pain and bicytopenia: Trouble once again with these IgM antibodies!
[So] Source:J Clin Virol;75:60-3, 2016 Feb.
[Is] ISSN:1873-5967
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We here report the case of a 30-year old man with a history of ulcerative colitis, who presented clinical and biological features compatible with a viral hepatitis. Initial serological results revealed the presence of IgM antibodies against many viruses, and the most likely diagnosis was viral hepatitis A. However, further investigations were performed and concluded to cytomegalovirus primary infection.
[Mh] Termos MeSH primário: Colite Ulcerativa
Infecções por Citomegalovirus
Citomegalovirus
Imunoglobulina M/sangue
[Mh] Termos MeSH secundário: Dor Abdominal
Adulto
Anticorpos Antivirais/imunologia
Colite Ulcerativa/sangue
Colite Ulcerativa/complicações
Colite Ulcerativa/imunologia
Colite Ulcerativa/fisiopatologia
Infecções por Citomegalovirus/complicações
Infecções por Citomegalovirus/diagnóstico
Diagnóstico Diferencial
Febre
Hepatite A/complicações
Hepatite A/diagnóstico
Seres Humanos
Imunoglobulina M/imunologia
Masculino
Sorologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Immunoglobulin M)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160131
[Lr] Data última revisão:
160131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151219
[St] Status:MEDLINE


  10 / 726 MEDLINE  
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[PMID]:24944047
[Au] Autor:Grzybowski A; Ciesielska M
[Ad] Endereço:Department of Ophthalmology, Poznan City Hospital, University Medical School, Poznan, Poland.
[Ti] Título:Lesser known aspects of Ludwik Fleck's (1896-1961) heroic life during World War II.
[So] Source:J Med Biogr;24(3):402-8, 2016 Aug.
[Is] ISSN:1758-1087
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Professor Ludwik Fleck was a famous scientist and a prominent philosopher. Although his life and work were studied extensively, the Second World War period was a subject of some discussion and controversy. On account of his Jewish origin, he was first arrested and moved from the Lwów ghetto to the 'Laokoon' factory and then imprisoned in KL Auschwitz-Birkenau and in KL Buchenwald. Fleck produced the anti-typhus vaccine in the chemo-bacteriological laboratory in the Jewish Hospital at Kuszewicza Street and in the 'Laokoon' factory in Lwów. During his incarceration in KL Auschwitz-Birkenau, Fleck worked in the camp laboratory in Block 10 carrying out bacteriological studies for the inmates and then was assigned to work in the Wasserman station in Rajsko. From January 1944 Fleck performed routine laboratory tests in Block 50 in KL Buchenwald. Though Fleck had a privileged life in the camp, he participated in the sabotage activities organized by the camp resistance.
[Mh] Termos MeSH primário: Alergia e Imunologia/história
Microbiologia/história
Vacinas Antirrickéttsia/história
[Mh] Termos MeSH secundário: História do Século XX
Filosofia
Polônia
Sorologia/história
II Guerra Mundial
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Ps] Nome de pessoa como assunto:Fleck L
[Nm] Nome de substância:
0 (Rickettsial Vaccines); 0 (typhus vaccine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM; QIS
[Da] Data de entrada para processamento:140620
[St] Status:MEDLINE
[do] DOI:10.1177/0967772014532893



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