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[PMID]:29229330
[Au] Autor:Guan F; You Y; Li X; Robinson MA
[Ad] Endereço:Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center Campus, 382 West Street Road, Kennett Square, PA, 19348, USA; Pennsylvania Equine Toxicology and Research Laboratory, 220 East Rosedale Avenue, West Chester, PA, 19382, USA. Electronic addres
[Ti] Título:Detection and confirmation of α-cobratoxin in equine plasma by solid-phase extraction and liquid chromatography coupled to mass spectrometry.
[So] Source:J Chromatogr A;1533:38-48, 2018 Jan 19.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:α-Cobratoxin (CTX) is a large peptide (71 amino acids) with strong analgesic effect and may be misused in sports such as horse racing. To prevent such misuse, a sensitive method is required for detection and confirmation of the toxin in equine samples. CTX was extracted from equine plasma using an optimized mixed-mode solid-phase extraction (SPE) procedure. Extracted CTX was reduced with dithiothreitol and alkylated with iodoacetamide, and then was digested by trypsin at 56°C for 30min. The digest was analysed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), and tryptic peptides T2 ( CFITPDITSK ) and T4 ( TWCDAFCSIR ) were monitored for detection and confirmation of CTX. The limit of detection (LOD) was 0.05ng/mL for CTX in plasma, and the limit of confirmation (LOC) 0.2ng/mL. Unlike small peptides consisting of the 20 canonical amino acids, CTX was stable in equine plasma at ambient temperature for at least 24h. The developed analytical method was successfully applied to analysis of incurred plasma samples; CTX was detected in plasma collected 15min through 36h post subcutaneous administration of CTX (2.0mg dose) to a research horse, and confirmed 30min through 24h. Additionally, an approach named "reliable targeted SEQUEST search" has been proposed for assessing the specificity of T2 at product ion spectrum level for confirmation of CTX. T2 is uniquely specific for CTX, as evaluated with this approach and BLAST search. Furthermore, the effect of dimethyl sulfoxide (DMSO) as a mobile phase additive on electrospray (ESI) response of T2 and T4, background noise level and signal to noise ratio (S/N) was examined; DMSO increased signal intensity of T2 and T4 by a factor of less than 2. It is the first report that DMSO raised background noise level and did not improve S/N for the peptides, to the authors' knowledge. The developed analytical method may be applicable for analysis of CTX in plasma from other species such as greyhound dogs or even human beings.
[Mh] Termos MeSH primário: Análise Química do Sangue/métodos
Cromatografia Líquida
Proteínas Neurotóxicas de Elapídeos/sangue
Doping nos Esportes/prevenção & controle
Extração em Fase Sólida
Espectrometria de Massas em Tandem
[Mh] Termos MeSH secundário: Animais
Análise Química do Sangue/normas
Cavalos
Limite de Detecção
Preparações Farmacêuticas/sangue
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cobra Neurotoxin Proteins); 0 (Pharmaceutical Preparations); 69344-74-7 (alpha-cobratoxin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


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[PMID]:28939282
[Au] Autor:Cuervo D; Loli C; Fernández-Álvarez M; Muñoz G; Carreras D
[Ad] Endereço:Laboratorio de Control del Dopaje, Agencia Española de Protección de la Salud en el Deporte, Ministerio de Educación, Cultura y Deporte, Gobierno de España, c/El Greco s/n, 28040, Madrid, Spain. Electronic address: dario.cuervo@aepsad.gob.es.
[Ti] Título:Determination of doping peptides via solid-phase microelution and accurate-mass quadrupole time-of-flight LC-MS.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1065-1066:134-144, 2017 Oct 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A complete analytical protocol for the determination of 25 doping-related peptidic drugs and 3 metabolites in urine was developed by means of accurate-mass quadrupole time-of-flight (Q-TOF) LC-MS analysis following solid-phase extraction (SPE) on microplates and conventional SPE pre-treatment for initial testing and confirmation, respectively. These substances included growth hormone releasing factors, gonadotropin releasing factors and anti-diuretic hormones, with molecular weights ranging from 540 to 1320Da. Optimal experimental conditions were stablished after investigation of different parameters concerning sample preparation and instrumental analysis. Weak cation exchange SPE followed by C18 HPLC chromatography and accurate mass detection provided the required sensitivity and selectivity for all the target peptides under study. 2mg SPE on 96-well microplates can be used in combination with full scan MS detection for the initial testing, thus providing a fast, cost-effective and high-throughput protocol for the processing of a large batch of samples simultaneously. On the other hand, extraction on 30mg SPE cartridges and subsequent target MS/MS determination was the protocol of choice for confirmatory purposes. The methodology was validated in terms of selectivity, recovery, matrix effect, precision, sensitivity (limit of detection, LOD), cross contamination, carryover, robustness and stability. Recoveries ranged from 6 to 70% (microplates) and 17-95% (cartridges), with LODs from 0.1 to 1ng/mL. The suitability of the method was assessed by analyzing different spiked or excreted urines containing some of the target substances.
[Mh] Termos MeSH primário: Doping nos Esportes
Peptídeos/urina
Extração em Fase Sólida/métodos
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Antidiuréticos/isolamento & purificação
Antidiuréticos/urina
Cromatografia Líquida de Alta Pressão/métodos
Hormônio Liberador de Gonadotropina/isolamento & purificação
Hormônio Liberador de Gonadotropina/urina
Hormônio Liberador de Hormônio do Crescimento/isolamento & purificação
Hormônio Liberador de Hormônio do Crescimento/urina
Seres Humanos
Limite de Detecção
Peptídeos/isolamento & purificação
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antidiuretic Agents); 0 (Peptides); 33515-09-2 (Gonadotropin-Releasing Hormone); 9034-39-3 (Growth Hormone-Releasing Hormone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170924
[St] Status:MEDLINE


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[PMID]:28850889
[Au] Autor:Abushareeda W; Lyris E; Kraiem S; Wahaibi AA; Alyazidi S; Dbes N; Lommen A; Nielen M; Horvatovich PL; Alsayrafi M; Georgakopoulos C
[Ad] Endereço:Anti-Doping Lab Qatar, Sports City, P.O. Box. 27775, Doha, Qatar.
[Ti] Título:Gas chromatographic quadrupole time-of-flight full scan high resolution mass spectrometric screening of human urine in antidoping analysis.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1063:74-83, 2017 Sep 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This paper presents the development and validation of a high-resolution full scan (FS) electron impact ionization (EI) gas chromatography coupled to quadrupole Time-of-Flight mass spectrometry (GC/QTOF) platform for screening anabolic androgenic steroids (AAS) in human urine samples. The World Antidoping Agency (WADA) enlists AAS as prohibited doping agents in sports, and our method has been developed to comply with the qualitative specifications of WADA to be applied for the detection of sports antidoping prohibited substances, mainly for AAS. The method also comprises of the quantitative analysis of the WADA's Athlete Biological Passport (ABP) endogenous steroidal parameters. The applied preparation of urine samples includes enzymatic hydrolysis for the cleavage of the Phase II glucuronide conjugates, generic liquid-liquid extraction and trimethylsilyl (TMS) derivatization steps. Tandem mass spectrometry (MS/MS) acquisition was applied on few selected ions to enhance the specificity and sensitivity of GC/TOF signal of few compounds. The full scan high resolution acquisition of analytical signal, for known and unknown TMS derivatives of AAS provides the antidoping system with a new analytical tool for the detection designer drugs and novel metabolites, which prolongs the AAS detection, after electronic data files' reprocessing. The current method is complementary to the respective liquid chromatography coupled to mass spectrometry (LC/MS) methodology widely used to detect prohibited molecules in sport, which cannot be efficiently ionized with atmospheric pressure ionization interface.
[Mh] Termos MeSH primário: Anabolizantes/urina
Doping nos Esportes/prevenção & controle
Cromatografia Gasosa-Espectrometria de Massas/métodos
[Mh] Termos MeSH secundário: Androsterona/urina
Criança
Epitestosterona/urina
Feminino
Seres Humanos
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anabolic Agents); 481-30-1 (Epitestosterone); C24W7J5D5R (Androsterone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE


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[PMID]:28700478
[Au] Autor:Xing Y; Liu X; Yan M; Chen T; Lu F; Xu B; Gong Y; Chu F; Lei H
[Ad] Endereço:aSchool of Chinese Pharmacy, Beijing University of Chinese Medicine bNational Anti-Doping Laboratory, China Anti-Doping Agency cCollege of Applied Statistics, Beijing University of Technology, Beijing, China.
[Ti] Título:Impact of nonsteroidal aromatase inhibitors on steroid profile in a Chinese population.
[So] Source:Medicine (Baltimore);96(28):e7411, 2017 Jul.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Steroid profiling was introduced to determine the endogenous steroid misuse in sports. Thus, screening for the exogenous use of these prohibited substances can be established by monitoring a range of endogenous steroids, which constitute the steroid profile and evaluate their concentrations and ratios against reference values. The steroid profiling is currently based on population statistics. As large interindividual variations exist, athlete biological passport (ABP) analysis is ongoing. This study aimed to identify new biomarker(s) for aromatase inhibitor detection in sports using statistical analysis and adapt the model into ABP analysis.Forty-one Chinese nonathlete volunteers (21 males and 20 females) were administered 3 nonsteroidal aromatase inhibitors (aminoglutethimide, letrozole, and anastrozole) independently. Statistical analysis was performed on 16 steroid profile parameters.After administration, the concentrations of endogenous androgen biomarkers including testosterone (T), epitestosterone, androsterone (AN), etiocholanolone (ETIO), 5α-diol, 5ß-diol, and dehydroepiandrosterone were increased, while the level of estrogen was decreased. These biomarkers returned to the baselines levels within 1 month. In females, the concentrations of endogenous biomarkers were affected by nonsteroidal aromatase inhibitors, without a common trend. Three new endogenous biomarkers (AN/estrone, ETIO/estrone, and T/estrone) elevated significantly after treatment. The 3 new models were more sensitive than the World Anti-Doping Agency ratio biomarkers. They were also effective in exponentially weighted moving average chart analysis.Verification experiment demonstrated that the biomarker T/estrone was valid in judging the steroidal aromatase inhibitor abuse. The screening of these new endogenous biomarkers can provide additional parameters to support ABP monitoring and specific information regarding the administered steroids.
[Mh] Termos MeSH primário: Aminoglutetimida/farmacologia
Inibidores da Aromatase/farmacologia
Hormônios/sangue
Nitrilos/farmacologia
Esteroides/sangue
Triazóis/farmacologia
[Mh] Termos MeSH secundário: Biomarcadores Farmacológicos/sangue
Doping nos Esportes
Feminino
Seres Humanos
Masculino
Modelos Biológicos
Método Simples-Cego
Transtornos Relacionados ao Uso de Substâncias/sangue
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Aromatase Inhibitors); 0 (Biomarkers, Pharmacological); 0 (Hormones); 0 (Nitriles); 0 (Steroids); 0 (Triazoles); 0O54ZQ14I9 (Aminoglutethimide); 2Z07MYW1AZ (anastrozole); 7LKK855W8I (letrozole)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170730
[Lr] Data última revisão:
170730
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000007411


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[PMID]:28674304
[Au] Autor:Imanishi T; Kawabata T; Takayama A
[Ad] Endereço:Education and Research Center for Clinical Pharmacy, Kyoto Pharmaceutical University.
[Ti] Título:Current Status of Doping in Japan Based on Japan Anti-Doping Disciplinary Panels of the Japan Anti-Doping Agency (JADA): A Suggestion on Anti-Doping Activities by Pharmacists in Japan.
[So] Source:Yakugaku Zasshi;137(7):883-891, 2017.
[Is] ISSN:1347-5231
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:In 2009, the Japan Anti-Doping Agency (JADA) established the "Sports Pharmacist Accreditation Program" to prevent doping in sports. Since then, anti-doping activities in Japan have been attracting attention. In this study, we investigated research about the current status of doping from 2007 to 2014 in Japan to make anti-doping activities more concrete, and we also discussed future anti-doping activities by pharmacists. In Japan, bodybuilding was the sporting event with the highest number and rate of doping from 2007 to 2014. Many of the positive doping cases were detected for class S1 (anabolic agents), S5 (diuretics and masking agents), and S6 (stimulants). Within class S1, supplements were the main cause of positive doping. Within class S5, medicines prescribed by medical doctors were the main cause of positive doping. Within class S6, non-prescription medicines (e.g., OTC) were the main cause of positive doping. When we looked at the global statistics on doping, many of the positive doping cases were detected for class S1. On comparing the Japanese statistics with the global statistics, the rate of positive doping caused by class S1 was significantly lower, but that caused by classes S5 and S6 was significantly higher in Japan than in the world. In conclusion, pharmacists in Japan should pay attention to class S1, S5, and S6 prohibited substances and to the sport events of bodybuilding. Based on this study, sports pharmacists as well as common pharmacists should suggest new anti-doping activities to prevent doping in the future.
[Mh] Termos MeSH primário: Doping nos Esportes/prevenção & controle
Doping nos Esportes/estatística & dados numéricos
Farmacêuticos
Papel Profissional
[Mh] Termos MeSH secundário: Anabolizantes/efeitos adversos
Estimulantes do Sistema Nervoso Central/efeitos adversos
Diuréticos/efeitos adversos
Doping nos Esportes/tendências
Glucocorticoides/efeitos adversos
Seres Humanos
Japão/epidemiologia
Detecção do Abuso de Substâncias
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anabolic Agents); 0 (Central Nervous System Stimulants); 0 (Diuretics); 0 (Glucocorticoids)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170705
[St] Status:MEDLINE
[do] DOI:10.1248/yakushi.16-00260


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[PMID]:28533317
[Au] Autor:Baggish AL; Weiner RB; Kanayama G; Hudson JI; Lu MT; Hoffmann U; Pope HG
[Ad] Endereço:From Cardiovascular Performance Program, Division of Cardiology, Massachusetts General Hospital, Boston (A.L.B., R.B.W.); Department of Medicine, Harvard Medical School, Boston, MA (A.L.B., R.B.W.); Biological Psychiatry Laboratory and Psychiatric Epidemiology Research Program, McLean Hospital, Belm
[Ti] Título:Cardiovascular Toxicity of Illicit Anabolic-Androgenic Steroid Use.
[So] Source:Circulation;135(21):1991-2002, 2017 May 23.
[Is] ISSN:1524-4539
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Millions of individuals have used illicit anabolic-androgenic steroids (AAS), but the long-term cardiovascular associations of these drugs remain incompletely understood. METHODS: Using a cross-sectional cohort design, we recruited 140 experienced male weightlifters 34 to 54 years of age, comprising 86 men reporting ≥2 years of cumulative lifetime AAS use and 54 nonusing men. Using transthoracic echocardiography and coronary computed tomography angiography, we assessed 3 primary outcome measures: left ventricular (LV) systolic function (left ventricular ejection fraction), LV diastolic function (early relaxation velocity), and coronary atherosclerosis (coronary artery plaque volume). RESULTS: Compared with nonusers, AAS users demonstrated relatively reduced LV systolic function (mean±SD left ventricular ejection fraction = 52±11% versus 63±8%; <0.001) and diastolic function (early relaxation velocity = 9.3±2.4 cm/second versus 11.1±2.0 cm/second; <0.001). Users currently taking AAS at the time of evaluation (N=58) showed significantly reduced LV systolic (left ventricular ejection fraction = 49±10% versus 58±10%; <0.001) and diastolic function (early relaxation velocity = 8.9±2.4 cm/second versus 10.1±2.4 cm/second; =0.035) compared with users currently off-drug (N=28). In addition, AAS users demonstrated higher coronary artery plaque volume than nonusers (median [interquartile range] 3 [0, 174] mL versus 0 [0, 69] mL ; =0.012). Lifetime AAS dose was strongly associated with coronary atherosclerotic burden (increase [95% confidence interval] in rank of plaque volume for each 10-year increase in cumulative duration of AAS use: 0.60 SD units [0.16-1.03 SD units]; =0.008). CONCLUSIONS: Long-term AAS use appears to be associated with myocardial dysfunction and accelerated coronary atherosclerosis. These forms of AAS-associated adverse cardiovascular phenotypes may represent a previously underrecognized public-health problem.
[Mh] Termos MeSH primário: Androgênios/efeitos adversos
Doença da Artéria Coronariana/induzido quimicamente
Vasos Coronários/efeitos dos fármacos
Doping nos Esportes
Substâncias para Melhoria do Desempenho/efeitos adversos
Transtornos Relacionados ao Uso de Substâncias/complicações
Congêneres da Testosterona/efeitos adversos
Disfunção Ventricular Esquerda/induzido quimicamente
Função Ventricular Esquerda/efeitos dos fármacos
Levantamento de Peso
[Mh] Termos MeSH secundário: Adulto
Cardiotoxicidade
Estudos de Casos e Controles
Angiografia por Tomografia Computadorizada
Angiografia Coronária/métodos
Doença da Artéria Coronariana/diagnóstico por imagem
Doença da Artéria Coronariana/patologia
Vasos Coronários/diagnóstico por imagem
Vasos Coronários/patologia
Estudos Transversais
Diástole
Ecocardiografia
Seres Humanos
Masculino
Meia-Idade
Tomografia Computadorizada Multidetectores
Placa Aterosclerótica
Medição de Risco
Fatores de Risco
Volume Sistólico/efeitos dos fármacos
Sístole
Fatores de Tempo
Disfunção Ventricular Esquerda/diagnóstico por imagem
Disfunção Ventricular Esquerda/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Androgens); 0 (Performance-Enhancing Substances); 0 (Testosterone Congeners)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE
[do] DOI:10.1161/CIRCULATIONAHA.116.026945


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[PMID]:28504570
[Au] Autor:Gröschl M
[Ad] Endereço:Celerion Switzerland AG, Allmendstrasse 32, CH-8320 Fehraltorf, Switzerland.
[Ti] Título:Saliva: a reliable sample matrix in bioanalytics.
[So] Source:Bioanalysis;9(8):655-668, 2017 Apr.
[Is] ISSN:1757-6199
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Saliva is gaining increasing attention as a bioanalytical sample matrix. Mostly because of the easy and noninvasive collection, it is not only beneficial in endocrinological and behavioral science, but also in pediatrics. Saliva also has the advantage of being the only body fluid which can be collected even during physical exercise, for example, during sportive activities, and there are physiological characteristics that make it superior to serum/plasma or urine for specific scientific questions. This review provides an insight into the physiology of saliva formation, explaining how certain compounds enter this bodily fluid, and gives advice for collection, storage and analytical methods. Finally, it presents a number of reliable and proven applications for saliva analysis from scientific fields including endocrinology, sports medicine, forensics and immunology.
[Mh] Termos MeSH primário: Saliva/química
Saliva/fisiologia
Manejo de Espécimes/métodos
[Mh] Termos MeSH secundário: Animais
Biomarcadores/análise
Cromatografia/métodos
Doping nos Esportes
Monitoramento de Medicamentos/métodos
Desenho de Equipamento
Ciências Forenses/métodos
Hormônios/análise
Seres Humanos
Imunoglobulinas/análise
Técnicas Imunológicas/métodos
Preservação Biológica/métodos
Glândulas Salivares/anatomia & histologia
Glândulas Salivares/química
Glândulas Salivares/fisiologia
Manejo de Espécimes/instrumentação
Medicina Esportiva/métodos
Detecção do Abuso de Substâncias/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biomarkers); 0 (Hormones); 0 (Immunoglobulins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.4155/bio-2017-0010


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[PMID]:28387807
[Au] Autor:Liu Y; Uboh CE; Li X; Guan F; You Y; Maylin GA; Zhu F; Soma LR
[Ad] Endereço:Department of Clinical Studies, University of Pennsylvania School of Veterinary Medicine, New Bolton Center Campus, 382 West Street Road, Kennett Square, PA 19348, USA.
[Ti] Título:Validated LC-MS-MS Method for Simultaneous Analysis of 17 Barbiturates in Horse Plasma for Doping Control.
[So] Source:J Anal Toxicol;41(5):431-440, 2017 Jun 01.
[Is] ISSN:1945-2403
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A rapid and sensitive method for simultaneous screening, quantification and confirmation of 17 barbiturates in horse plasma using liquid chromatography-tandem mass spectrometry is described. Analytes were recovered from plasma by liquid-liquid extraction using methyl tert-butyl ether, separated on a C18 column, and analyzed in negative electrospray ionization mode. Multiple-reaction monitoring was employed for screening and quantification. Confirmation for the presence of the analytes was achieved by comparing ion intensity ratio. The ranges for limits of detection, quantification and confirmation were 0.003-1 ng/mL (S/N ≥ 3), 0.01-2.5 ng/mL and 0.02-5 ng/mL, respectively. The linear dynamic range of the method was 0.1-100 ng/mL. The precision and accuracy at 0.5, 5 and 50 ng/mL of all 17 barbiturates during intra-day assay were 1.6-8.6% and 96-106%, respectively. For inter-day assay, precision and accuracy at the same three concentrations were 2.6-8.9% and 96-106%, respectively. Analysis of all 17 analytes was completed within 7 min. Thus, the present method is fast, simple, sensitive and reproducibly reliable.
[Mh] Termos MeSH primário: Barbitúricos/sangue
Doping nos Esportes
Detecção do Abuso de Substâncias/métodos
[Mh] Termos MeSH secundário: Animais
Cavalos
Extração Líquido-Líquido
Éteres Metílicos
Plasma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Barbiturates); 0 (Methyl Ethers); 29I4YB3S89 (methyl tert-butyl ether)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170721
[Lr] Data última revisão:
170721
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1093/jat/bkx025


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[PMID]:28385722
[Au] Autor:Collier R
[Ad] Endereço:CMAJ.
[Ti] Título:A place for pot in sports?
[So] Source:CMAJ;189(11):E448-E449, 2017 03 20.
[Is] ISSN:1488-2329
[Cp] País de publicação:Canada
[La] Idioma:eng
[Mh] Termos MeSH primário: Analgésicos Opioides/uso terapêutico
Atletas
Traumatismos em Atletas/tratamento farmacológico
Doping nos Esportes
Maconha Medicinal/uso terapêutico
Dor/tratamento farmacológico
[Mh] Termos MeSH secundário: Traumatismos em Atletas/complicações
Canadá
Seres Humanos
Dor/etiologia
[Pt] Tipo de publicação:NEWS
[Nm] Nome de substância:
0 (Analgesics, Opioid); 0 (Medical Marijuana)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1503/cmaj.1095401


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[PMID]:28382919
[Au] Autor:Herholz C; Zink N; Laska H; Gumpendobler M; Trolliet C; Probst S
[Ad] Endereço:Hochschule für Agrar-, Forst- und Lebensmittelwissenschaften, Zollikofen.
[Ti] Título:[Doping relevant substances in horse feed].
[Ti] Título:Dopingrelevante Substanzen in Futtermitteln für Pferde..
[So] Source:Schweiz Arch Tierheilkd;159(4):231-235, 2017 Apr.
[Is] ISSN:0036-7281
[Cp] País de publicação:Switzerland
[La] Idioma:ger
[Ab] Resumo:INTRODUCTION: Horse feed material of Swiss and foreign production available on the Swiss market was tested with regard to possible contamination with 7 different alkaloids, atropine and colchicine. Twenty-eight feed samples as well as 2 poppy seed samples were analyzed. Out of 28 feed samples 18 were positive for prohibited substances in Equestrian sports. The concentration of prohibited substances was in none of the samples high enough to cause an effect on the body of the horse. The poppy seed sample, which was obtained by conventional harvesting and cleaning methods, contained a very high Morphine concentration compared to the sample which was harvested by hand. Despite high quality standards a contamination with prohibited substances in horse feed cannot be excluded.
[Mh] Termos MeSH primário: Ração Animal/análise
Ração Animal/normas
Doping nos Esportes
Contaminação de Alimentos/análise
Cavalos/fisiologia
[Mh] Termos MeSH secundário: Alcaloides/análise
Animais
Atropina/análise
Colchicina/análise
Morfina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 76I7G6D29C (Morphine); 7C0697DR9I (Atropine); SML2Y3J35T (Colchicine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.17236/sat00112



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