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[PMID]:29220517
[Au] Autor:Varenhorst AJ; Pritchard SR; O'Neal ME; Hodgson EW; Singh AK
[Ad] Endereço:Plant Science Department, South Dakota State University.
[Ti] Título:Determining the Effectiveness of Three-Gene Pyramids Against Aphis glycines (Hemiptera: Aphididae) Biotypes.
[So] Source:J Econ Entomol;110(6):2428-2435, 2017 12 05.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Since the discovery of Aphis glycines Matsumura (Hemiptera: Aphididae) in the United States, the primary management tactic has been foliar insecticides. Alternative management options such as host plant resistance to A. glycines have been developed and their effectiveness proved. However, the use of host plant resistance was complicated by the discovery of multiple, virulent biotypes of A. glycines in the United States that are capable of overcoming single Rag genes, Rag1 and Rag2, as well as a two-gene pyramid of Rag1+Rag2. However, current models predict that the virulent allele frequency of A. glycines decreases in response to the use of pyramided Rag genes, suggesting that pyramids represent a more sustainable use of these traits. Previous research has demonstrated that virulent biotypes can be effectively managed using a three-gene pyramid of Rag1+Rag2+Rag3. Additional Rag-genes have been discovered (Rag4 and Rag5), but whether the incorporation of these genes into novel three-gene pyramids will improve efficacy is not known. We tested single-gene (Rag1 and Rag2) and pyramid cultivars (Rag1+Rag2, Rag1+Rag2+Rag3, Rag1+Rag2+Rag4) to multiple biotypes in laboratory assays. Our results confirm that the Rag1+Rag2+Rag3 pyramid effectively manages all known A. glycines biotypes when compared with cultivars that are overcome by the associated biotype. Our results indicate that Rag1+Rag2+Rag4 would be an effective management option for biotype-1, biotype-2, and biotype-3 A. glycines, but had a negligible impact on biotype-4.
[Mh] Termos MeSH primário: Afídeos/fisiologia
Herbivoria
Controle Biológico de Vetores/métodos
Feijão de Soja/genética
[Mh] Termos MeSH secundário: Animais
Fenótipo
Melhoramento Vegetal
Feijão de Soja/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tox230


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[PMID]:29372803
[Au] Autor:Rysbekova AB; Kazkeyev DT; Usenbekov BN; Mukhina ZM; Zhanbyrbaev EA; Sartbaeva IA; Zhambakin KZ; Berkimbay KA; Batayeva DS
[Ti] Título:[Prebreeding selection of rice with colored pericarp based on genotyping Rc and Pb genes].
[So] Source:Genetika;53(1):43-53, 2017 Jan.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The research was aimed at developing prebreeding resources of Kazakhstan rice varieties with colored pericarp for breeding. During the study, hybrid analysis of inheritance of the trait "colored pericarp" in breeding material used for the work was performed. Rice genotypes with colored pericarp, as well as white rice varieties possessing important breeding traits and maturing under conditions of the republic, were selected from the collection of the Institute of Plant Biology and Biotechnology, Republic of Kazakhstan. Identification of allelic status of Rc (red pericarp) and Pb (anthocyanin pericarp) genes was performed for selected samples using the PCR method. When selecting parental forms for crossing, foreign rice varieties with colored pericarp (Rubin, Mavr, Black rice, etc.) were used as recipient forms. As donors, we used local white rice varieties of Kazakhstan breeding adapted to the soil and climate conditions of rice growing regions (Madina, Marzhan, Bakanasskiy, PakLi) as well as foreign varieties. The ability to set hybrid caryopses and the percentage of sterility were determined in obtained F1 rice hybrids. As a result, the most promising prebreeding material was selected, which will be used for breeding Kazakhstan rice varieties with colored pericarp.
[Mh] Termos MeSH primário: Genes de Plantas/fisiologia
Genótipo
Oryza
Pigmentação/genética
Melhoramento Vegetal
Sementes
[Mh] Termos MeSH secundário: Oryza/genética
Oryza/metabolismo
Sementes/genética
Sementes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE


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[PMID]:29314836
[Au] Autor:Lee J; Nonaka S; Takayama M; Ezura H
[Ad] Endereço:Graduate School of Life and Environmental Sciences, University of Tsukuba , 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
[Ti] Título:Utilization of a Genome-Edited Tomato (Solanum lycopersicum) with High Gamma Aminobutyric Acid Content in Hybrid Breeding.
[So] Source:J Agric Food Chem;66(4):963-971, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:γ-Aminobutyric acid (GABA) is a nonproteogenic amino acid with health-promoting functions. Although tomato fruits have a relatively high GABA content compared with other crops, levels must be further increased to effectively confer the health-promoting functions. In this study, we evaluated the potential of the genome-edited tomato as a breeding material for producing high-GABA hybrid tomatoes. Hybrid lines were produced by crossing the genome-edited tomato with a pure line tomato cultivar, "Aichi First", and were evaluated for GABA accumulation and other fruit traits. The hybrid lines showed high GABA accumulation in the fruits, which was sufficiently high for expecting health-promoting functions and had minimal effects on other fruit traits, suggesting that the high GABA is a dominant trait and that the genome-edited tomato would be useful as a parental line of hybrid cultivars. These results also indicate that genome editing technology is useful for the rapid breeding of high-GABA hybrid tomato cultivars.
[Mh] Termos MeSH primário: Hibridização Genética
Lycopersicon esculentum/química
Lycopersicon esculentum/genética
Melhoramento Vegetal/métodos
Plantas Geneticamente Modificadas
Ácido gama-Aminobutírico/análise
[Mh] Termos MeSH secundário: Alelos
Sequência de Aminoácidos
Aminoácidos/análise
Carotenoides/análise
Edição de Genes
Genes de Plantas
Glutamato Descarboxilase/química
Glutamato Descarboxilase/genética
Lycopersicon esculentum/metabolismo
Mutação
Plantas Geneticamente Modificadas/genética
Ácido gama-Aminobutírico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 36-88-4 (Carotenoids); 56-12-2 (gamma-Aminobutyric Acid); EC 4.1.1.15 (Glutamate Decarboxylase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05171


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[PMID]:29369585
[Au] Autor:Lebedeva MV; Levkoev EA; Volkov VA; Fetisova AA; Navalikhin SV; Shabunin DA; Danilov YI; Zhigunov AV; Potokin EK
[Ti] Título:[The recovering of breeding achievements of Populus × leningradensis bogd. and Populus × newensis bogd. Based on microsatellite analysis].
[So] Source:Genetika;52(10):1159-68, 2016 Oct.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The genotyping of 75 trees from poplar plantations in St. Petersburg and Leningrad oblast was conducted with microsatellite markers to identify the elite clonal varieties developed by P.L. Bogdanov in the period of 1938­1965. The information about the varieties was lost. The authentic herbarium specimens of poplar clonal varieties preserved at the St. Petersburg State Forest Technical University were used as reference genotypes. According to the results of DNA fingerprinting, we identified the clonal plantations of Populus × newesis Bogd. and Populus × leningradensis Bogd. from the Kartashevskii forest district and the arboretum of the St. Petersburg State Forest Technical University. The identified elite poplar hybrids have a higher frost resistant and a higher growth rate. They are recommended for plantation cultivation in the northwest of Russia.
[Mh] Termos MeSH primário: Aclimatação
Genótipo
Repetições de Microssatélites
Melhoramento Vegetal
Populus/genética
[Mh] Termos MeSH secundário: Técnicas de Genotipagem
Populus/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


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[PMID]:29369563
[Au] Autor:Traykov M; Trenchev I
[Ti] Título:Mathematical models in genetics.
[So] Source:Genetika;52(9):1089-96, 2016 Sep.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:In this study, we present some of the basic ideas of population genetics. The founders of population genetics are R.A. Fisher, S. Wright, and J. B.S. Haldane. They, not only developed almost all the basic theory associated with genetics, but they also initiated multiple experiments in support of their theories. One of the first significant insights, which are a result of the Hardy­Weinberg law, is Mendelian inheritance preserves genetic variation on which the natural selection acts. We will limit to simple models formulated in terms of differential equations. Some of those differential equations are nonlinear and thus emphasize issues such as the stability of the fixed points and time scales on which those equations operate. First, we consider the classic case when selection acts on diploid locus at which wу can get arbitrary number of alleles. Then, we consider summaries that include recombination and selection at multiple loci. Also, we discuss the evolution of quantitative traits. In this case, the theory is formulated in respect of directly measurable quantities. Special cases of this theory have been successfully used for many decades in plants and animals breeding.
[Mh] Termos MeSH primário: Modelos Genéticos
Plantas/genética
[Mh] Termos MeSH secundário: Animais
Cruzamento
Loci Gênicos
Melhoramento Vegetal
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


  6 / 743 MEDLINE  
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[PMID]:29368884
[Au] Autor:Lantos C; Pauk J
[Ti] Título:Anther culture as an effective tool in winter wheat (Triticum aestivum L.) breeding.
[So] Source:Genetika;52(8):910-8, 2016 Aug.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to determine the effect of genotype and induction medium in anther culture of wheat (Triticum aestivum L.). Ten F1 winter wheat genotypes were tested in anther culture (AC) to compare the two most frequently applied induction media (W14mf and P4mf). Androgenesis was induced during the treatment of each tested genotypes and green plants were produced from them using both media. Based on statistical analysis, the genotypes significantly influenced (at the 0.001 probability level) the efficiency of AC (embryo-like structures (ELS), albinos, green plantlets and transplanted plantlets) and the media also had a significant effect on the number of ELS and albino plantlets. Both media can be used for AC in wheat doubled haploid (DH) plant production. The production of ELS and green plantlets was higher in P4mf medium (48.84 ELS/100 anthers, 4.82 green plantlets/100 anthers) than in W14mf medium (28.14 ELS/100 anthers, 4.59 green plantlets/100 anthers). However, the green plant regeneration efficiency of the microspore-derived structures was 16.9% when using W14mf medium, while this value was 9.6% in the case of ELS induced with P4mf medium. The application of W14mf medium thus proved to be time- and labour-saving medium in the large-scale production of DH wheat plants. In our experiments, 267 DH plants were produced for our winter wheat breeding program. The spontaneous rediploidization rate was 32.72%.
[Mh] Termos MeSH primário: Genótipo
Melhoramento Vegetal/métodos
Técnicas de Embriogênese Somática de Plantas/métodos
Triticum
[Mh] Termos MeSH secundário: Triticum/genética
Triticum/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


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[PMID]:29368867
[Au] Autor:Afonnikov DA; Genaev MA; Doroshkov AV; Komyshev EG; Pshenichnikova TA
[Ti] Título:[Methods of high-throughput plant phenotyping for large-scale breeding and genetic experiments].
[So] Source:Genetika;52(7):788-803, 2016 Jul.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Phenomics is a field of science at the junction of biology and informatics which solves the problems of rapid, accurate estimation of the plant phenotype; it was rapidly developed because of the need to analyze phenotypic characteristics in large scale genetic and breeding experiments in plants. It is based on using the methods of computer image analysis and integration of biological data. Owing to automation, new approaches make it possible to considerably accelerate the process of estimating the characteristics of a phenotype, to increase its accuracy, and to remove a subjectivism (inherent to humans). The main technologies of high-throughput plant phenotyping in both controlled and field conditions, their advantages and disadvantages, and also the prospects of their use for the efficient solution of problems of plant genetics and breeding are presented in the review.
[Mh] Termos MeSH primário: Processamento de Imagem Assistida por Computador
Fenótipo
Melhoramento Vegetal/métodos
Plantas/anatomia & histologia
Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


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[PMID]:29368840
[Au] Autor:Khlestkina EK; Shumny VK
[Ti] Título:[Prospects for application of breakthrough technologies in breeding: The CRISPR/Cas9 system for plant genome editing].
[So] Source:Genetika;52(7):774-87, 2016 Jul.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Integration of the methods of contemporary genetics and biotechnology into the breeding process is assessed, and the potential role and efficacy of genome editing as a novel approach is discussed. Use of molecular (DNA) markers for breeding was proposed more than 30 years ago. Nowadays, they are widely used as an accessory tool in order to select plants by mono- and olygogenic traits. Presently, the genomic approaches are actively introduced into the breeding processes owing to automatization of DNA polymorphism analyses and development of comparatively cheap methods of DNA sequencing. These approaches provide effective selection by complex quantitative traits, and are based on the full-genome genotyping of the breeding material. Moreover, biotechnological tools, such as doubled haploids production, which provides fast obtainment of homozygotes, are widely used in plant breeding. Use of genomic and biotechnological approaches makes the development of varieties less time consuming. It also decreases the cultivated areas and financial expenditures required for accomplishment of the breeding process. However, the capacities of modern breeding are not limited to only these advantages. Experiments carried out on plants about 10 years ago provided the first data on genome editing. In the last two years, we have observed a sharp increase in the number of publications that report about successful experiments aimed at plant genome editing owing to the use of the relatively simple and convenient CRISPR/Cas9 system. The goal of some of these experiments was to modify agriculturally valuable genes of cultivated plants, such as potato, cabbage, tomato, maize, rice, wheat, barley, soybean and sorghum. These studies show that it is possible to obtain nontransgenic plants carrying stably inherited, specifically determined mutations using the CRISPR/Cas9 system. This possibility offers the challenge to obtain varieties with predetermined mono- and olygogenic traits.
[Mh] Termos MeSH primário: Sistemas CRISPR-Cas
Edição de Genes/métodos
Genoma de Planta
Melhoramento Vegetal
Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


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[PMID]:29228905
[Au] Autor:Kwong QB; Teh CK; Ong AL; Chew FT; Mayes S; Kulaveerasingam H; Tammi M; Yeoh SH; Appleton DR; Harikrishna JA
[Ad] Endereço:Biotechnology & Breeding Department, Sime Darby Plantation R&D Centre, 43400, Serdang, Selangor, Malaysia. kwong.qi.bin@simedarbyplantation.com.
[Ti] Título:Evaluation of methods and marker Systems in Genomic Selection of oil palm (Elaeis guineensis Jacq.).
[So] Source:BMC Genet;18(1):107, 2017 Dec 11.
[Is] ISSN:1471-2156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Genomic selection (GS) uses genome-wide markers as an attempt to accelerate genetic gain in breeding programs of both animals and plants. This approach is particularly useful for perennial crops such as oil palm, which have long breeding cycles, and for which the optimal method for GS is still under debate. In this study, we evaluated the effect of different marker systems and modeling methods for implementing GS in an introgressed dura family derived from a Deli dura x Nigerian dura (Deli x Nigerian) with 112 individuals. This family is an important breeding source for developing new mother palms for superior oil yield and bunch characters. The traits of interest selected for this study were fruit-to-bunch (F/B), shell-to-fruit (S/F), kernel-to-fruit (K/F), mesocarp-to-fruit (M/F), oil per palm (O/P) and oil-to-dry mesocarp (O/DM). The marker systems evaluated were simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs). RR-BLUP, Bayesian A, B, Cπ, LASSO, Ridge Regression and two machine learning methods (SVM and Random Forest) were used to evaluate GS accuracy of the traits. RESULTS: The kinship coefficient between individuals in this family ranged from 0.35 to 0.62. S/F and O/DM had the highest genomic heritability, whereas F/B and O/P had the lowest. The accuracies using 135 SSRs were low, with accuracies of the traits around 0.20. The average accuracy of machine learning methods was 0.24, as compared to 0.20 achieved by other methods. The trait with the highest mean accuracy was F/B (0.28), while the lowest were both M/F and O/P (0.18). By using whole genomic SNPs, the accuracies for all traits, especially for O/DM (0.43), S/F (0.39) and M/F (0.30) were improved. The average accuracy of machine learning methods was 0.32, compared to 0.31 achieved by other methods. CONCLUSION: Due to high genomic resolution, the use of whole-genome SNPs improved the efficiency of GS dramatically for oil palm and is recommended for dura breeding programs. Machine learning slightly outperformed other methods, but required parameters optimization for GS implementation.
[Mh] Termos MeSH primário: Arecaceae/genética
Marcadores Genéticos
Genoma de Planta
Melhoramento Vegetal/métodos
Seleção Genética
[Mh] Termos MeSH secundário: Arecaceae/crescimento & desenvolvimento
Teorema de Bayes
Mapeamento Cromossômico
Cromossomos de Plantas
Genômica
Genótipo
Repetições de Microssatélites
Fenótipo
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1186/s12863-017-0576-5


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[PMID]:29272307
[Au] Autor:Technow F; Gerke J
[Ad] Endereço:Maize Product Development/Systems and Innovation for Breeding and Seed Products, DuPont Pioneer, Tavistock, Ontario, Canada.
[Ti] Título:Parent-progeny imputation from pooled samples for cost-efficient genotyping in plant breeding.
[So] Source:PLoS One;12(12):e0190271, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The increased usage of whole-genome selection (WGS) and other molecular evaluation methods in plant breeding relies on the ability to genotype a very large number of untested individuals in each breeding cycle. Many plant breeding programs evaluate large biparental populations of homozygous individuals derived from homozygous parent inbred lines. This structure lends itself to parent-progeny imputation, which transfers the genotype scores of the parents to progeny individuals that are genotyped for a much smaller number of loci. Here we introduce a parent-progeny imputation method that infers individual genotypes from non-barcoded pooled samples of DNA of multiple individuals using a Hidden Markov Model (HMM). We demonstrate the method for pools of simulated maize double haploids (DH) from biparental populations, genotyped using a genotyping by sequencing (GBS) approach for 3,000 loci at 0.125x to 4x coverage. We observed high concordance between true and imputed marker scores and the HMM produced well-calibrated genotype probabilities that correctly reflected the uncertainty of the imputed scores. Genomic estimated breeding values (GEBV) calculated from the imputed scores closely matched GEBV calculated from the true marker scores. The within-population correlation between these sets of GEBV approached 0.95 at 1x and 4x coverage when pooling two or four individuals, respectively. Our approach can reduce the genotyping cost per individual by a factor up to the number of pooled individuals in GBS applications without the need for extra sequencing coverage, thereby enabling cost-effective large scale genotyping for applications such as WGS in plant breeding.
[Mh] Termos MeSH primário: Análise Custo-Benefício
Melhoramento Vegetal
[Mh] Termos MeSH secundário: DNA de Plantas/genética
Sequenciamento de Nucleotídeos em Larga Escala
Cadeias de Markov
Modelos Teóricos
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171223
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190271



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