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[PMID]:29478634
[Au] Autor:Pagés-Díaz J; Pereda-Reyes I; Sanz JL; Lundin M; Taherzadeh MJ; Horváth IS
[Ad] Endereço:Process Engineering Centre (CIPRO), Universidad Tecnológica de La Habana "José Antonio Echeverría" (Technical University of Havana "José A. Echeverría"), (Cujae), Marianao, La Habana, Cuba; Swedish Centre for Resource Recovery, University of Borås, 501 90 Borås, Sweden. Electronic address: jhosane@q
[Ti] Título:A comparison of process performance during the anaerobic mono- and co-digestion of slaughterhouse waste through different operational modes.
[So] Source:J Environ Sci (China);64:149-156, 2018 Feb.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The use of consecutive feeding was applied to investigate the response of the microbial biomass to a second addition of substrates in terms of biodegradation using batch tests as a promising alternative to predict the behavior of the process. Anaerobic digestion (AD) of the slaughterhouse waste (SB) and its co-digestion with manure (M), various crops (VC), and municipal solid waste were evaluated. The results were then correlated to previous findings obtained by the authors for similar mixtures in batch and semi-continuous operation modes. AD of the SB failed showing total inhibition after a second feeding. Co-digestion of the SB+M showed a significant improvement for all of the response variables investigated after the second feeding, while co-digestion of the SB+VC resulted in a decline in all of these response variables. Similar patterns were previously detected, during both the batch and the semi-continuous modes.
[Mh] Termos MeSH primário: Matadouros
Resíduos Industriais
Eliminação de Resíduos/métodos
[Mh] Termos MeSH secundário: Anaerobiose
Biodegradação Ambiental
Reatores Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Industrial Waste)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180227
[St] Status:MEDLINE


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[PMID]:29107193
[Au] Autor:Feurer C; Le Roux A; Rossel R; Barnaud E; Dumarest M; Garry P; Pavio N
[Ad] Endereço:IFIP, French Institute for the Pig and Pork Industry, La Motte au Vicomte, B.P. 35104, 35651 Le Rheu, Cedex, France. Electronic address: carole.feurer@ifip.asso.fr.
[Ti] Título:High load of hepatitis E viral RNA in pork livers but absence in pork muscle at French slaughterhouses.
[So] Source:Int J Food Microbiol;264:25-30, 2018 Jan 02.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Pork ham muscle can be contaminated with HEV via blood vessels during viremia and represents a possible source of human contamination via the consumption of dried ham. This study evaluated the prevalence of HEV RNA in pork ham muscles and pork livers at slaughterhouses. Serology was determined on the corresponding serum samples. The apparent individual seroprevalence rate in the 49 pig farms studied was 59% [55.5%-61.4%]. None of the 1134 ham muscles tested was positive for the presence of HEV. HEV prevalence in paired liver samples was 2.8% with a level of contamination of up to 1.46 10 copies/g. Sequences of viral strains isolated from positive livers belonged to genotype 3 and subtypes 3c, 3e, 3f and 3j. Our results confirmed that raw pork liver food products are a source of risk for humans but they also showed that there is a limited risk of human infection by HEV through the consumption of ham muscle.
[Mh] Termos MeSH primário: Vírus da Hepatite E/isolamento & purificação
Hepatite E/epidemiologia
Músculos/virologia
Carne Vermelha/virologia
Sus scrofa/virologia
Doenças dos Suínos/epidemiologia
[Mh] Termos MeSH secundário: Matadouros
Adulto
Animais
França/epidemiologia
Genótipo
Vírus da Hepatite E/genética
Seres Humanos
Fígado/virologia
RNA Viral/genética
Estudos Soroepidemiológicos
Suínos
Doenças dos Suínos/virologia
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171107
[St] Status:MEDLINE


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[PMID]:28454589
[Au] Autor:Beyene T; Hayishe H; Gizaw F; Beyi AF; Abunna F; Mammo B; Ayana D; Waktole H; Abdi RD
[Ad] Endereço:Department of Biomedical Sciences, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia. takele.beyene@aau.edu.et.
[Ti] Título:Prevalence and antimicrobial resistance profile of Staphylococcus in dairy farms, abattoir and humans in Addis Ababa, Ethiopia.
[So] Source:BMC Res Notes;10(1):171, 2017 Apr 28.
[Is] ISSN:1756-0500
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Staphylococcus species cause mastitis and wound infection in livestock and food poisoning in humans through ingestion of contaminated foods, including meat and dairy products. They are evolving pathogens in that they readily acquire drug resistance, and multiple drug-resistant (MDR) isolates are increasing in human and veterinary healthcare. Therefore, this study was conducted to evaluate the prevalence of Staphylococci and their drug resistance in dairy farms and abattoir settings of Addis Ababa. METHODS: In this cross-sectional study, 193 samples of milk, meat, equipment and humans working in the dairy farms and abattoir were collected (dairy farms = 72 and abattoir sources = 121). Staphylococcus isolation and identification at the species level was done according to ISO-6888-3 using biochemical characteristics. An antimicrobial susceptibility test was conducted for 43 of the isolates using 15 antimicrobial agents commonly used for humans and livestock by the Kirby Bauer disk diffusion method following CLSI guidelines. RESULTS: Staphylococcus organism were isolated from 92 (47.7%) of the total 193 samples, 50% in the dairy farms and 46.3% in the abattoir. The isolated species were S. aureus (n = 31; 16.1%), S. intermedius (n = 21; 10.9%), S. hyicus (n = 16; 8.3%), and coagulase negative Staphylococcus (CNS) (n = 24; 12.4%). Gentamycin was effective drug as all isolates (n = 43; 100%) were susceptible to it and followed by kanamycin (n = 39; 90.7%). However, the majority of the isolates showed resistance to penicillin-G (95.3%), nalidixic acid (88.4%), cloxacillin (79.1%), vancomycin (65.1%) and cefoxitin (55.8%). Of the 15 S. aureus tested for drug susceptibility, 73.3% of them were phenotypically resistant to vancomycin (VRSA) and all of the 15 isolates showed multi-drug resistance (MDR) to >3 drugs. Also, all of the tested CNS (100%), S. hyicus (100%) and the majority of S. intermedius isolates (88.9%) developed MDR. CONCLUSION: Alarmingly, the Staphylococcus isolates circulating in the dairy farms and abattoir in the study area harbor MDR. High level of Staphylococcus species isolation from personnel and equipment besides food (meat and milk) samples in dairy farms and abattoir settings reveals that the hygiene practice in the dairy farm and abattoir is substandard. Prudent drug use and improved hygienic practice is recommended in the dairy farms and abattoir to safeguard the public from the risk of acquiring infections and MDR pathogenic Staphylococcus.
[Mh] Termos MeSH primário: Farmacorresistência Bacteriana/genética
Mastite Bovina/epidemiologia
Staphylococcus aureus Resistente à Meticilina/genética
Saúde do Trabalhador/educação
Infecções Estafilocócicas/epidemiologia
Staphylococcus/genética
[Mh] Termos MeSH secundário: Matadouros
Criação de Animais Domésticos
Animais
Antibacterianos/farmacologia
Técnicas de Tipagem Bacteriana
Bovinos
Estudos Transversais
Etiópia/epidemiologia
Fazendas
Feminino
Gentamicinas/farmacologia
Seres Humanos
Canamicina/farmacologia
Mastite Bovina/microbiologia
Mastite Bovina/transmissão
Carne/microbiologia
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Testes de Sensibilidade Microbiana
Leite/microbiologia
Prevalência
Infecções Estafilocócicas/microbiologia
Infecções Estafilocócicas/transmissão
Staphylococcus/classificação
Staphylococcus/efeitos dos fármacos
Staphylococcus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Gentamicins); 59-01-8 (Kanamycin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1186/s13104-017-2487-y


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[PMID]:28449278
[Au] Autor:Bansal M; Kaur S; Deka D; Singh R; Gill JPS
[Ad] Endereço:School of Public Health and Zoonoses, GADVASU, Ludhiana, India.
[Ti] Título:Seroepidemiology and molecular characterization of hepatitis E virus infection in swine and occupationally exposed workers in Punjab, India.
[So] Source:Zoonoses Public Health;64(8):662-672, 2017 12.
[Is] ISSN:1863-2378
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Hepatitis E virus (HEV) has two discrete epidemiological patterns: waterborne epidemics in developing countries only, caused by HEV genotype I, and sporadic zoonotic outbreaks in developing and developed countries caused by genotypes III and IV. This study was designed to investigate seroprevalence, molecular detection and the characterization of HEV by nested RT-PCR in swine as well as the occupational risk to exposed human population in Punjab state of north-western India. The occupational risk-exposed group comprised of swine farmers (organized - mixed feed feeders and unorganized - swill feeders), slaughterhouse workers, sewage workers and veterinary internes. During the study period, blood and faecal samples were collected from 320 swine and 360 humans with both high and low occupational exposure risks. The overall seroprevalence of swine HEV was 65.00%, with a significantly higher seropositivity in growing pigs (2-8 months of age). The prevalence of HEV RNA in swine faecal samples by nRT-PCR was 8.75% with a significantly higher detection in swill-fed pigs. With humans in the high occupational exposure risk population, significantly higher anti-HEV IgG seropositivity was observed (60.48%) as compared to control population (10.71%). Strong evidence of association between human anti-HEV IgG seropositivity and certain occupational exposure risk groups was observed (p < 0.05). This indicates that unorganized swine farmers, slaughterhouse workers and sewage workers have higher odds of HEV infection in this study region. Percentage of nucleotide similarity between swine and human HEV isolates was less than that found in countries with zoonotic HEV outbreaks. Molecular characterization revealed the circulation of G IV and G I genotypes among swine and human population in Punjab state, respectively.
[Mh] Termos MeSH primário: Vírus da Hepatite E
Hepatite E/veterinária
Exposição Ocupacional
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Matadouros
Adulto
Animais
Fazendeiros
Fezes/virologia
Hepatite E/epidemiologia
Hepatite E/virologia
Vírus da Hepatite E/genética
Seres Humanos
Índia/epidemiologia
Filogenia
Estudos Soroepidemiológicos
Suínos
Doenças dos Suínos/epidemiologia
Médicos Veterinários
Adulto Jovem
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1111/zph.12363


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[PMID]:27770505
[Au] Autor:Rodgers JD; Simpkin E; Lee R; Clifton-Hadley FA; Vidal AB
[Ad] Endereço:Department of Bacteriology, Animal and Plant Health Agency, Addlestone, Surrey, UK.
[Ti] Título:Sensitivity of Direct Culture, Enrichment and PCR for Detection of Campylobacter jejuni and C. coli in Broiler Flocks at Slaughter.
[So] Source:Zoonoses Public Health;64(4):262-271, 2017 06.
[Is] ISSN:1863-2378
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Broiler chicken flocks are a significant source of Campylobacter jejuni and Campylobacter coli that result in the major public health problem of campylobacteriosis. Accurate estimates of the prevalence of both C. coli and C. jejuni in flocks would enhance epidemiological understanding, risk assessment and control options. This study combined results from a panel of 10 detection tests (direct culture, enrichment and PCR) on caecal samples from flocks at slaughter. A parallel interpretation approach was used to determine the presence of Campylobacter spp. and for C. jejuni and C. coli individually. The sample was considered positive if at least one method detected the target and this interpretation was taken to represent a 'proxy gold standard' for detection in the absence of a gold standard reference test. The sensitivity of each individual method to detect Campylobacter spp., C. jejuni and C. coli was then estimated relative to the proxy gold standard. Enrichment in adapted Exeter broth (deficient in polymyxin B) with a resuscitation step was 100% sensitive, whilst direct culture on modified charcoal cefoperazone deoxycholate agar (mCCDA) was highly sensitive (97.9%). Enrichment methods using Preston broth and Bolton broth were significantly less sensitive. Enrichment in Exeter broth promoted the recovery of C. jejuni, whilst enrichment in Bolton broth favoured C. coli. A RT-PCR detection test could identify 80% of flocks that were co-colonised with both species. This study found that 76.3% (n = 127) of flocks were colonised with Campylobacter spp. The majority (95.9%) of Campylobacter-positive flocks were colonised with C. jejuni; however, approximately one-third of positive flocks were simultaneously colonised with both C. jejuni and C. coli. The findings highlight the impact of different detection methodologies on the accuracy of the estimated incidence of both C. jejuni and C. coli entering the abattoir within broiler flocks and the associated public health risks.
[Mh] Termos MeSH primário: Técnicas Bacteriológicas/veterinária
Infecções por Campylobacter/veterinária
Campylobacter coli/isolamento & purificação
Campylobacter jejuni/isolamento & purificação
Galinhas/microbiologia
Doenças das Aves Domésticas/microbiologia
[Mh] Termos MeSH secundário: Matadouros
Animais
Infecções por Campylobacter/microbiologia
Reação em Cadeia da Polimerase/métodos
Reação em Cadeia da Polimerase/veterinária
Doenças das Aves Domésticas/diagnóstico
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE
[do] DOI:10.1111/zph.12306


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[PMID]:29284248
[Au] Autor:Dutkiewicz J; Sroka J; Zajac V; Wasinski B; Cisak E; Sawczyn A; Kloc A; Wójcik-Fatla A
[Ad] Endereço:Department of Biological Health Hazards and Parasitology, Institute of Rural Health, Lublin, Poland. jadut777@onet.eu.
[Ti] Título:Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part I - Epidemiology.
[So] Source:Ann Agric Environ Med;24(4):683-695, 2017 Dec 23.
[Is] ISSN:1898-2263
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Streptococcus suis (ex Elliot 1966, Kilpper-Bälz & Schleifer 1987) is a facultatively anaerobic Gram-positive ovoid or coccal bacterium surrounded by a polysaccharide capsule. Based on the antigenic diversity of the capsule, S. suis strains are classified serologically into 35 serotypes. Streptococcus suis is a commensal of pigs, commonly colonizing their tonsils and nasal cavities, mostly in weaning piglets between 4-10 weeks of age. This species occurs also in cattle and other mammals, in birds and in humans. Some strains, mostly those belonging to serotype 2, are also pathogenic for pigs, as well as for other animals and humans. Meningitis is the primary disease syndrome caused by S. suis, both in pigs and in humans. It is estimated that meningitis accounted for 68.0% of all cases of human disease reported until the end of 2012, followed by septicaemia (including life-threatening condition described as 'streptococcal toxic shock-like syndrome' - STSLS), arthritis, endocarditis, and endophthalmitis. Hearing loss and/or ves tibular dysfunction are the most common sequelae after recovery from meningitis caused by S. suis, occurring in more than 50% of patients. In the last two decades, the number of reported human cases due to S. suis has dramatically increased, mostly due to epidemics recorded in China in 1998 and 2005, and the fulminant increase in morbidity in the countries of south-eastern Asia, mostly Vietnam and Thailand. Out of 1,642 cases of S. suis infections identified between 2002-2013 worldwide in humans, 90.2% occurred in Asia, 8.5% in Europe and 1.3% in other parts of the globe. The human disease has mostly a zoonotic and occupational origin and occurs in pig breeders, abattoir workers, butchers and workers of meat processing facilities, veterinarians and meat inspectors. Bacteria are transmitted to workers by close contact with pigs or pig products, usually through contamination of minor cuts or abrasions on skin of hands and/or arms, or by pig bite. A different epidemiologic situation occurs in the Southeast Asian countries where most people become infected by habitual consumption of raw or undercooked pork, blood and offal products in the form of traditional dishes. Prevention of S. suis infections in pigs includes vaccination, improvement in pig-raising conditions, disinfection and/or fumigation of animal houses, and isolation of sick animals at the outbreak of disease. Prevention of human infections comprises: protection of skin from pig bite or injury with sharp tools by people occupationally exposed to pigs and pig products, prompt disinfection and dressing of wounds and abrasions at work, protection of the respiratory tract by wearing appropriate masks or repirators, consulting a doctor in the case of febrile illness after exposure to pigs or pork meat, avoidance of occupations associated with exposure to pigs and pork by immunocompomised people, avoidance of consumption of raw pork or pig blood, adequate cooking of pork, and health education.
[Mh] Termos MeSH primário: Produtos da Carne/microbiologia
Exposição Ocupacional/análise
Infecções Estreptocócicas/epidemiologia
Infecções Estreptocócicas/veterinária
Streptococcus suis/fisiologia
Doenças dos Suínos/epidemiologia
[Mh] Termos MeSH secundário: Matadouros
Animais
China
Seres Humanos
Produtos da Carne/análise
Infecções Estreptocócicas/microbiologia
Streptococcus suis/genética
Streptococcus suis/isolamento & purificação
Suínos
Doenças dos Suínos/microbiologia
Zoonoses/epidemiologia
Zoonoses/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE


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[PMID]:28585329
[Au] Autor:Domínguez R; Pateiro M; Crecente S; Ruiz M; Sarriés MV; Lorenzo JM
[Ad] Endereço:Centro Tecnológico de la Carne de Galicia, Parque Tecnológico de Galicia, Ourense, Spain.
[Ti] Título:Effect of linseed supplementation and slaughter age on meat quality of grazing cross-bred Galician x Burguete foals.
[So] Source:J Sci Food Agric;98(1):266-273, 2018 Jan.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The aim of this study was to assess the effect of finishing diet (control concentrate vs. linseed concentrate) and slaughter age (13 vs. 26 months) on meat and nutritional quality of foal meat. For this study, 46 foals from crossing Galicia Mountain x Burguete breeds were used. RESULTS: The obtained results showed that slaughter age had an influence on chemical composition and colour parameters. Foals slaughtered at the age of 13 months had lower content of intramuscular fat and higher cholesterol contents than those slaughtered at 26 months of age. Regarding colour parameters, older foals showed the highest values of redness and lowest myoglobin contents. Finishing diet had a low effect on the majority of parameters evaluated. Foals fed with linseed presented lower shear force values than those fed with control concentrate. Fatty acid and amino acid contents were hardly influenced by finishing diet, whereas slaughter age effect had a high impact on fatty acid profile. Older animals showed lowest SFA values and n-6/n-3 ratio. CONCLUSION: Older animals presented the best meat quality. Diet had low effect in meat quality and could be related to the short time during finishing diet was administered and the low amount of linseed in the experimental diet. © 2017 Society of Chemical Industry.
[Mh] Termos MeSH primário: Ração Animal/análise
Suplementos Nutricionais/análise
Linho/química
Cavalos/metabolismo
Óleo de Semente do Linho/análise
Carne/análise
[Mh] Termos MeSH secundário: Matadouros
Aminoácidos/análise
Aminoácidos/metabolismo
Animais
Cruzamento
Feminino
Linho/metabolismo
Cavalos/genética
Cavalos/crescimento & desenvolvimento
Óleo de Semente do Linho/metabolismo
Masculino
Músculo Esquelético/química
Músculo Esquelético/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 8001-26-1 (Linseed Oil)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170607
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8466


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[PMID]:29255538
[Au] Autor:Ayoola MC; Akinseye VO; Cadmus E; Awosanya E; Popoola OA; Akinyemi OO; Perrett L; Taylor A; Stack J; Moriyon I; Cadmus SI
[Ad] Endereço:Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.
[Ti] Título:Prevalence of bovine brucellosis in slaughtered cattle and barriers to better protection of abattoir workers in Ibadan, South-Western Nigeria.
[So] Source:Pan Afr Med J;28:68, 2017.
[Is] ISSN:1937-8688
[Cp] País de publicação:Uganda
[La] Idioma:eng
[Ab] Resumo:Introduction: Brucellosis is a neglected zoonosis of public health importance. This study was conducted to determine the prevalence and risk factors of brucellosis among slaughtered cattle as well as challenges to the protection of abattoir workers in Nigeria. Methods: A slaughterhouse study was conducted in a major abattoir in Ibadan from March to August, 2013. To diagnose brucellosis, serum samples from 1,241 slaughtered cattle were tested using Rose-Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (cELISA); again, 57 milk samples were tested with milk ring test (MRT) and indirect ELISA (iELISA). Furthermore, a survey on the usage of personal protective equipment (PPE) and challenges to its use by abattoir workers was done. Data were analysed using Stata 12. Results: Seroprevalence by RBT was 7.8%; 77.3% (75/97) of these were corroborated by cELISA. Prevalence in milk samples by MRT and indirect ELISA were 33.3% and 3.5%, respectively. Sex (OR: 2.5; 95%CI:1.3-4.5) was the factor significantly associated with seropositivity. None of the abattoir workers used standard protective overalls; while, 99.6% of the meat handlers and 84.1% of the butchers worked barefoot. Most of the workers (75.7%) wore no protective gloves. The respondents agreed that provision of free PPE and sanctions against non-users would encourage its use. Conclusion: Our findings indicate moderate prevalence (7.8%) of bovine brucellosis with sex of cattle being a risk factor. A notable barrier to better protection of abattoir workers against brucellosis is perceived inconvenience arising from use of gloves. Therefore, preventive and control measures against brucellosis must include education and use of PPE among abattoir workers.
[Mh] Termos MeSH primário: Matadouros
Brucella/isolamento & purificação
Brucelose/epidemiologia
Doenças Profissionais/prevenção & controle
[Mh] Termos MeSH secundário: Adulto
Animais
Brucelose/prevenção & controle
Brucelose/veterinária
Bovinos
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Masculino
Nigéria/epidemiologia
Doenças Profissionais/microbiologia
Exposição Ocupacional/efeitos adversos
Prevalência
Roupa de Proteção/utilização
Fatores de Risco
Estudos Soroepidemiológicos
Fatores Sexuais
Zoonoses/microbiologia
Zoonoses/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171222
[Lr] Data última revisão:
171222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.11604/pamj.2017.28.68.10925


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[PMID]:28462735
[Au] Autor:Dohmen W; VAN Gompel L; Schmitt H; Liakopoulos A; Heres L; Urlings BA; Mevius D; Bonten MJM; Heederik DJJ
[Ad] Endereço:Division of Environmental Epidemiology,Institute for Risk Assessment Sciences, Utrecht University,Utrecht,the Netherlands.
[Ti] Título:ESBL carriage in pig slaughterhouse workers is associated with occupational exposure.
[So] Source:Epidemiol Infect;145(10):2003-2010, 2017 07.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We investigated the prevalence of extended-spectrum ß-lactamase (ESBL) carriage in slaughterhouse workers and the association with occupational exposure to slaughter animals and products. Stool samples from 334 employees in a Dutch pig slaughterhouse were obtained. Presence of ESBL was determined by selective plating, microarray analysis, and gene sequencing. Questionnaires were used to collect personal and occupational information. The overall prevalence of ESBL carriage was 4·8% (16/334). All ESBL-producing isolates were Escherichia coli. The ESBL genes detected were bla CTX-M-1 (n = 8), bla CTX-M-15 (n = 3), bla CTX-M-27 (n = 2), bla CTX-M-24 (n = 1), bla CTX-M-55 (n = 1), and bla SHV-12 (n = 1). A higher prevalence of ESBL was seen in workers in jobs with as tasks 'removal of lungs, heart, liver, tongue' (33%), and 'removal of head and spinal cord' (25%). For further analysis, participants were divided in two groups based on potential exposure to ESBL as related to their job title. One group with an assumed higher exposure to ESBL (e.g. stable work, stabbing, dehairing, removal of organs) and another group with an assumed lower exposure to ESBL (e.g. refrigeration, packaging and expedition). In the 'higher exposure' group, ten out of 95 (10·5%) were carrying ESBL vs. six out of 233 (2·6%) in the 'lower exposure' group. Human ESBL carriage was significantly associated with job exposure in the slaughterhouse (OR 4·5, CI 1·6-12·6). Results suggest that ESBL carriage in slaughterhouse workers overall is comparable with the Dutch population. Within the slaughterhouse population a difference in carriage exists depending on their position along the slaughter line and tasks involved.
[Mh] Termos MeSH primário: Matadouros
Infecções por Escherichia coli/epidemiologia
Escherichia coli/fisiologia
Exposição Ocupacional
[Mh] Termos MeSH secundário: Adulto
Animais
Escherichia coli/genética
Infecções por Escherichia coli/microbiologia
Fezes/microbiologia
Feminino
Seres Humanos
Masculino
Países Baixos/epidemiologia
Sus scrofa
beta-Lactamases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1017/S0950268817000784


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[PMID]:28829794
[Au] Autor:Brusa V; Restovich V; Galli L; Teitelbaum D; Signorini M; Brasesco H; Londero A; García D; Padola NL; Superno V; Sanz M; Petroli S; Costa M; Bruzzone M; Sucari A; Ferreghini M; Linares L; Suberbie G; Rodríguez R; Leotta GA
[Ad] Endereço:IGEVET-Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, La Plata, Argentina.
[Ti] Título:Isolation and characterization of non-O157 Shiga toxin-producing Escherichia coli from beef carcasses, cuts and trimmings of abattoirs in Argentina.
[So] Source:PLoS One;12(8):e0183248, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Several foods contaminated with Shiga toxin-producing Escherichia coli (STEC) are associated with human diseases. Some countries have established microbiological criteria for non-O157 STEC, thus, the absence of serogroups O26, O45, O103, O104, O111, O121, and O145 in sprouts from the European Union or ground beef and beef trimmings from the United States is mandatory. While in Argentina screening for O26, O103, O111, O145 and O121 in ground beef, ready-to-eat food, sausages and vegetables is mandatory, other countries have zero-tolerance for all STEC in chilled beef. The aim of this study was to provide data on the prevalence of non-O157 STEC isolated from beef processed in eight Argentinean cattle slaughterhouses producing beef for export and local markets, and to know the non-O157 STEC profiles through strain characterization and genotypic analysis. Samples (n = 15,965) from 3,205 beef carcasses, 9,570 cuts and 3,190 trimmings collected between March and September 2014 were processed in pools of five samples each. Pools of samples (n = 3,193) from 641 carcasses, 1,914 cuts and 638 trimming were analyzed for non-O157 STEC isolation according to ISO/CEN 13136:2012. Of these, 37 pools of carcasses (5.8%), 111 pools of cuts (5.8%) and 45 pools of trimmings (7.0%) were positive for non-O157 STEC. STEC strains (n = 200) were isolated from 193 pools of samples. The most prevalent serotypes were O174:H21, O185:H7, O8:H19, O178:H19 and O130:H11, and the most prevalent genotypes were stx2c(vh-b) and stx2a/saa/ehxA. O103:H21 strain was eae-positive and one O178:H19 strain was aggR/aaiC-positive. The prevalence of non-O157 STEC in beef carcasses reported here was low. None of the non-O157 STEC strains isolated corresponded to the non-O157 STEC serotypes and virulence profiles isolated from human cases in Argentina in the same study period. The application of microbiological criteria for each foodstuff should be determined by risk analysis in order to have a stringent monitoring system. Likewise, zero-tolerance intervention measures should be applied in beef, together with GMP and HACCP. Further, collaborative efforts for risk assessment, management and communication are extremely important to improve the safety of foodstuffs.
[Mh] Termos MeSH primário: Matadouros
Carne/microbiologia
Escherichia coli Shiga Toxigênica/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Argentina
Bovinos
Eletroforese em Gel de Campo Pulsado
Genes Bacterianos
Escherichia coli Shiga Toxigênica/química
Escherichia coli Shiga Toxigênica/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183248



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