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Pesquisa : J01.637.051.479.258 [Categoria DeCS]
Referências encontradas : 243 [refinar]
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[PMID]:29231937
[Au] Autor:Ugrinic M; Zambrano A; Berger S; Mann S; Tang TD; deMello A
[Ad] Endereço:Department of Chemistry & Applied Biosciences, ETH Zurich, Vladimir Prelog Weg 1, 8093 Zurich, Switzerland. andrew.demello@chem.ethz.ch.
[Ti] Título:Microfluidic formation of proteinosomes.
[So] Source:Chem Commun (Camb);54(3):287-290, 2018 Jan 02.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Herein we describe a novel microfluidic method for the generation of proteinosome micro-droplets, based on bovine serum albumin and glucose oxidase conjugated to PNIPAAm chains. The size of such water-in-oil droplets is regulated via control of the input reagent flow rate, with generated proteinosome populations exhibiting narrower size distributions than those observed when using standard bulk methodologies. Importantly, proteinosomes transferred from an oil to an aqueous-environment remain intact, become fully hydrated and exhibit an increase in average size. Moreover, functional proteinosomes prepared via microfluidics exhibit lower K values and higher enzymatic activities than proteinosomes produced by bulk methodologies.
[Mh] Termos MeSH primário: Células Artificiais/química
Glucose Oxidase/química
Soroalbumina Bovina/química
[Mh] Termos MeSH secundário: Resinas Acrílicas/química
Animais
Bovinos
Fluoresceína-5-Isotiocianato/química
Peroxidase do Rábano Silvestre/química
Microfluídica
Tamanho da Partícula
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acrylic Resins); 25189-55-3 (poly-N-isopropylacrylamide); 27432CM55Q (Serum Albumin, Bovine); EC 1.1.3.4 (Glucose Oxidase); EC 1.11.1.- (Horseradish Peroxidase); I223NX31W9 (Fluorescein-5-isothiocyanate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1039/c7cc08466h


  2 / 243 MEDLINE  
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[PMID]:28723913
[Au] Autor:Kinsler G; Sinai S; Lee NK; Nowak MA
[Ad] Endereço:Dept. Applied Mathematics, School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, United States of America.
[Ti] Título:Prebiotic selection for motifs in a model of template-free elongation of polymers within compartments.
[So] Source:PLoS One;12(7):e0180208, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The transition from prelife where self-replication does not occur, to life which exhibits self-replication and evolution, has been a subject of interest for many decades. Membranes, forming compartments, seem to be a critical component of this transition as they provide several concurrent benefits. They maintain localized interactions, generate electro-chemical gradients, and help in selecting cooperative functions as they arise. These functions pave the way for the emergence and maintenance of simple metabolic cycles and polymers. In the context of origin of life, evolution of information-carrying molecules and RNA based enzymes within compartments has been subject to intensive theoretical and experimental research. Hence, many experimental efforts aim to produce compartments that contain elongating polynucleotides (also referred to as protocells), which store information and perform catalysis. Despite impressive experimental progress, we are still relatively ignorant about the dynamics by which elongating polynucleotides can produce more sophisticated behaviors. Here we perform computer simulations to couple information production through template-free elongation of polymers with dividing compartments. We find that polymers with a simple ability-biasing the concentration of monomers within their own compartment-can acquire a selective advantage in prelife. We further investigate whether such a mechanism allows for cooperative dynamics to dominate over purely competitive ones. We show that under this system of biased monomer addition, even without template-directed self-replication, genetic motifs can emerge, compete, cooperate, and ultimately survive within the population.
[Mh] Termos MeSH primário: Evolução Biológica
Origem da Vida
Polímeros
Prebióticos
[Mh] Termos MeSH secundário: Células Artificiais
Simulação por Computador
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polymers); 0 (Prebiotics)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180208


  3 / 243 MEDLINE  
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[PMID]:28580796
[Au] Autor:Salehi-Reyhani A; Ces O; Elani Y
[Ad] Endereço:Department of Chemistry, Imperial College London, London SW7 2AZ, UK.
[Ti] Título:Artificial cell mimics as simplified models for the study of cell biology.
[So] Source:Exp Biol Med (Maywood);242(13):1309-1317, 2017 Jul.
[Is] ISSN:1535-3699
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Living cells are hugely complex chemical systems composed of a milieu of distinct chemical species (including DNA, proteins, lipids, and metabolites) interconnected with one another through a vast web of interactions: this complexity renders the study of cell biology in a quantitative and systematic manner a difficult task. There has been an increasing drive towards the utilization of artificial cells as cell mimics to alleviate this, a development that has been aided by recent advances in artificial cell construction. Cell mimics are simplified cell-like structures, composed from the bottom-up with precisely defined and tunable compositions. They allow specific facets of cell biology to be studied in isolation, in a simplified environment where control of variables can be achieved without interference from a living and responsive cell. This mini-review outlines the core principles of this approach and surveys recent key investigations that use cell mimics to address a wide range of biological questions. It will also place the field in the context of emerging trends, discuss the associated limitations, and outline future directions of the field. Impact statement Recent years have seen an increasing drive to construct cell mimics and use them as simplified experimental models to replicate and understand biological phenomena in a well-defined and controlled system. By summarizing the advances in this burgeoning field, and using case studies as a basis for discussion on the limitations and future directions of this approach, it is hoped that this minireview will spur others in the experimental biology community to use artificial cells as simplified models with which to probe biological systems.
[Mh] Termos MeSH primário: Células Artificiais
Fenômenos Fisiológicos Celulares
Técnicas Citológicas/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1177/1535370217711441


  4 / 243 MEDLINE  
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[PMID]:28358875
[Au] Autor:Ho KK; Lee JW; Durand G; Majumder S; Liu AP
[Ad] Endereço:Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, United States of America.
[Ti] Título:Protein aggregation with poly(vinyl) alcohol surfactant reduces double emulsion-encapsulated mammalian cell-free expression.
[So] Source:PLoS One;12(3):e0174689, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Development of artificial cell models requires encapsulation of biomolecules within membrane-bound compartments. There have been limited studies of using mammalian cell-free expression (CFE) system as the 'cytosol' of artificial cells. We exploit glass capillary droplet microfluidics for the encapsulation of mammalian CFE within double emulsion templated vesicles. The complexity of the physicochemical properties of HeLa cell-free lysate poses a challenge compared with encapsulating simple buffer solutions. In particular, we discovered the formation of aggregates in double emulsion templated vesicles encapsulating mammalian HeLa CFE, but not with bacterial CFE. The aggregates did not arise from insolubility of the proteins made from CFE nor due to the interaction of mammalian CFE with the organic solvents in the middle phase of the double emulsions. We found that aggregation is dependent on the concentration of poly(vinyl) alcohol (PVA) surfactant, a critical double emulsion-stabilizing surfactant, and the lysate concentration in mammalian CFE. Despite vesicle instability and reduced protein expression, we demonstrate protein expression by encapsulating mammalian CFE system. Using mass spectrometry and Western blot, we identified and verified that actin is one of the proteins inside the mammalian CFE that aggregated with PVA surfactant. Our work establishes a baseline description of mammalian CFE system encapsulated in double emulsion templated vesicles as a platform for building artificial cells.
[Mh] Termos MeSH primário: Sistema Livre de Células/química
Álcool de Polivinil/química
Agregados Proteicos
Solventes/química
[Mh] Termos MeSH secundário: Células Artificiais/química
Extratos Celulares/química
Vidro/química
Células HeLa
Seres Humanos
Microfluídica/métodos
Tamanho da Partícula
Surfactantes Pulmonares/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Extracts); 0 (Protein Aggregates); 0 (Pulmonary Surfactants); 0 (Solvents); 9002-89-5 (Polyvinyl Alcohol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174689


  5 / 243 MEDLINE  
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[PMID]:28282047
[Au] Autor:Mansy SS
[Ad] Endereço:Centre for Integrative Biology, University of Trento, via Sommarive 9, 38123 Povo TN, Italy.
[Ti] Título:Protocells: Non-living predators.
[So] Source:Nat Chem;9(2):107-108, 2017 01 24.
[Is] ISSN:1755-4349
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Células Artificiais/química
Biomimética/métodos
Biologia Sintética/métodos
[Mh] Termos MeSH secundário: DNA/química
Peptídeo Hidrolases/química
Eletricidade Estática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1038/nchem.2725


  6 / 243 MEDLINE  
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[PMID]:28280154
[Au] Autor:Mitchell LA; Wang A; Stracquadanio G; Kuang Z; Wang X; Yang K; Richardson S; Martin JA; Zhao Y; Walker R; Luo Y; Dai H; Dong K; Tang Z; Yang Y; Cai Y; Heguy A; Ueberheide B; Fenyö D; Dai J; Bader JS; Boeke JD
[Ad] Endereço:Department of Biochemistry and Molecular Pharmacology, New York University Langone School of Medicine, New York, NY 10016, USA.
[Ti] Título:Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond.
[So] Source:Science;355(6329), 2017 03 10.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We describe design, rapid assembly, and characterization of synthetic yeast Sc2.0 chromosome VI (synVI). A mitochondrial defect in the synVI strain mapped to synonymous coding changes within ( ), encoding an essential proteasome subunit; Sc2.0 coding changes reduced Pre4 protein accumulation by half. Completing Sc2.0 specifies consolidation of 16 synthetic chromosomes into a single strain. We investigated phenotypic, transcriptional, and proteomewide consequences of Sc2.0 chromosome consolidation in poly-synthetic strains. Another "bug" was discovered through proteomic analysis, associated with alteration of the transcription start due to transfer RNA deletion and loxPsym site insertion. Despite extensive genetic alterations across 6% of the genome, no major global changes were detected in the poly-synthetic strain "omics" analyses. This work sets the stage for completion of a designer, synthetic eukaryotic genome.
[Mh] Termos MeSH primário: Cromossomos Artificiais de Levedura/química
Cromossomos Artificiais de Levedura/genética
Saccharomyces cerevisiae/genética
Biologia Sintética/métodos
[Mh] Termos MeSH secundário: Células Artificiais/metabolismo
Mapeamento Físico do Cromossomo
Complexo de Endopeptidases do Proteassoma/genética
Proteômica
Saccharomyces cerevisiae/crescimento & desenvolvimento
Proteínas de Saccharomyces cerevisiae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Saccharomyces cerevisiae Proteins); EC 3.4.25.1 (PRE4 protein, S cerevisiae); EC 3.4.25.1 (Proteasome Endopeptidase Complex)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE


  7 / 243 MEDLINE  
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[PMID]:28187261
[Au] Autor:Timmer N; Droge ST
[Ad] Endereço:Institute for Risk Assessment Sciences, Utrecht University , Utrecht, 3508 TD, The Netherlands.
[Ti] Título:Sorption of Cationic Surfactants to Artificial Cell Membranes: Comparing Phospholipid Bilayers with Monolayer Coatings and Molecular Simulations.
[So] Source:Environ Sci Technol;51(5):2890-2898, 2017 Mar 07.
[Is] ISSN:1520-5851
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study reports the distribution coefficient between phospholipid bilayer membranes and phosphate buffered saline (PBS) medium (D ) for 19 cationic surfactants. The method used a sorbent dilution series with solid supported lipid membranes (SSLMs). The existing SSLM protocol, applying a 96 well plate setup, was adapted to use 1.5 mL glass autosampler vials instead, which facilitated sampling and circumvented several confounding loss processes for some of the cationic surfactants. About 1% of the phospholipids were found to be detached from the SSLM beads, resulting in nonlinear sorption isotherms for compounds with log D values above 4. Renewal of the medium resulted in linear sorption isotherms. D values determined at pH 5.4 demonstrated that cationic surfactant species account for the observed D . Log D values above 5.5 are only experimentally feasible with lower LC-MS/MS detection limits and/or concentrated extracts of the aqueous samples. Based on the number of carbon atoms, dialkylamines showed a considerably lower sorption affinity than linear alkylamine analogues. These SSLM results closely overlapped with measurements on a chromatographic tool based on immobilized artificial membranes (IAM-HPLC) and with quantum-chemistry based calculations with COSMOmic. The SSLM data suggest that IAM-HPLC underestimates the D of ionized primary and secondary alkylamines by 0.8 and 0.5 log units, respectively.
[Mh] Termos MeSH primário: Células Artificiais
Membranas Artificiais
[Mh] Termos MeSH secundário: Fosfolipídeos/química
Tensoativos
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membranes, Artificial); 0 (Phospholipids); 0 (Surface-Active Agents)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1021/acs.est.6b05662


  8 / 243 MEDLINE  
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[PMID]:28112394
[Au] Autor:Li J; Wang H; Kwon YC; Jewett MC
[Ad] Endereço:Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208.
[Ti] Título:Establishing a high yielding streptomyces-based cell-free protein synthesis system.
[So] Source:Biotechnol Bioeng;114(6):1343-1353, 2017 Jun.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cell-free protein synthesis (CFPS) has emerged as a powerful platform for applied biotechnology and synthetic biology, with a range of applications in synthesizing proteins, evolving proteins, and prototyping genetic circuits. To expand the current CFPS repertoire, we report here the development and optimization of a Streptomyces-based CFPS system for the expression of GC-rich genes. By developing a streamlined crude extract preparation protocol and optimizing reaction conditions, we were able to achieve active enhanced green fluorescent protein (EGFP) yields of greater than 50 µg/mL with batch reactions lasting up to 3 h. By adopting a semi-continuous reaction format, the EGFP yield could be increased to 282 ± 8 µg/mL and the reaction time was extended to 48 h. Notably, our extract preparation procedures were robust to multiple Streptomyces lividans and Streptomyces coelicolor strains, although expression yields varied. We show that our optimized Streptomyces lividans system provides benefits when compared to an Escherichia coli-based CFPS system for increasing percent soluble protein expression for four Streptomyces-originated high GC-content genes that are involved in biosynthesis of the nonribosomal peptides tambromycin and valinomycin. Looking forward, we believe that our Streptomyces-based CFPS system will contribute significantly towards efforts to express complex natural product gene clusters (e.g., nonribosomal peptides and polyketides), providing a new avenue for obtaining and studying natural product biosynthesis pathways. Biotechnol. Bioeng. 2017;114: 1343-1353. © 2017 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Vias Biossintéticas/genética
Melhoramento Genético/métodos
Biossíntese de Proteínas/fisiologia
Proteínas Recombinantes/biossíntese
Streptomyces/genética
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Células Artificiais/metabolismo
Proteínas Recombinantes/metabolismo
Streptomyces/classificação
Frações Subcelulares/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170607
[Lr] Data última revisão:
170607
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.1002/bit.26253


  9 / 243 MEDLINE  
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[PMID]:28110042
[Au] Autor:York-Duran MJ; Godoy-Gallardo M; Labay C; Urquhart AJ; Andresen TL; Hosta-Rigau L
[Ad] Endereço:Department of Micro- and Nanotechnology, Centre for Nanomedicine and Theranostics, DTU Nanotech, Technical University of Denmark, Building 423, 2800 Lyngby, Denmark.
[Ti] Título:Recent advances in compartmentalized synthetic architectures as drug carriers, cell mimics and artificial organelles.
[So] Source:Colloids Surf B Biointerfaces;152:199-213, 2017 Apr 01.
[Is] ISSN:1873-4367
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Compartmentalization is a key feature of biological cells which conduct their metabolic activity in individual steps isolated in distinct, separated compartments. The creation of architectures containing multiple compartments with a structure that resembles that of a biological cell has generated significant research attention and these assemblies are proposed as candidate materials for a range of biomedical applications. In this Review article, the recent successes of multicompartment architectures as carriers for the delivery of therapeutic cargo or the creation of micro- and nanoreactors that mimic metabolic activities, thus acting as artificial cells or organelles, are discussed. The developed technologies to assemble such complex architectures are outlined, the multicompartment carriers' properties which contribute to their performance in diverse applications are discussed, and their successful applications are highlighted. Finally, future directions and developments in the field are suggested.
[Mh] Termos MeSH primário: Portadores de Fármacos
[Mh] Termos MeSH secundário: Células Artificiais
Nanoestruturas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Drug Carriers)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170123
[St] Status:MEDLINE


  10 / 243 MEDLINE  
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[PMID]:28061350
[Au] Autor:Lipsitz YY; Bedford P; Davies AH; Timmins NE; Zandstra PW
[Ad] Endereço:Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada.
[Ti] Título:Achieving Efficient Manufacturing and Quality Assurance through Synthetic Cell Therapy Design.
[So] Source:Cell Stem Cell;20(1):13-17, 2017 Jan 05.
[Is] ISSN:1875-9777
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:New methods to manipulate gene and cell state can be used to engineer cell functionality, simplify quality assessment, and enhance manufacturability. These strategies could help overcome unresolved cell therapy manufacturing challenges and complement frameworks to design quality into these complex cellular systems, ultimately increasing patient access to living therapeutics.
[Mh] Termos MeSH primário: Células Artificiais/citologia
Terapia Baseada em Transplante de Células e Tecidos/normas
[Mh] Termos MeSH secundário: Engenharia Celular
Seres Humanos
Controle Social Formal
Pesquisa Médica Translacional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE



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