Base de dados : MEDLINE
Pesquisa : J01.637.087 [Categoria DeCS]
Referências encontradas : 6374 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 638 ir para página                         

  1 / 6374 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29193978
[Au] Autor:Balestri D; Roux Y; Mattarozzi M; Mucchino C; Heux L; Brazzolotto D; Artero V; Duboc C; Pelagatti P; Marchiò L; Gennari M
[Ad] Endereço:Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università degli Studi di Parma , Parco Area delle Scienze 17A, 43124 Parma, Italy.
[Ti] Título:Heterogenization of a [NiFe] Hydrogenase Mimic through Simple and Efficient Encapsulation into a Mesoporous MOF.
[So] Source:Inorg Chem;56(24):14801-14808, 2017 Dec 18.
[Is] ISSN:1520-510X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the quest for new, efficient, and noble-metal-free H -evolution catalysts, hydrogenase enzymes are a source of inspiration. Here, we describe the development of a new hybrid material based on a structural and functional [NiFe]-hydrogenase model complex (NiFe) incorporated into the Zr-based MOF PCN-777. The bulk NiFe@PCN-777 material was synthesized by simple encapsulation. Characterization by solid-state NMR and IR spectroscopy, SEM-EDX, ICP-OES, and gas adsorption confirmed the inclusion of the guest. FTO-supported thin films of the NiFe@PCN-777 composite were obtained by electrophoretic deposition of the bulk material and characterized by SEM-EDX, ICP-OES, and cyclic voltammetry. The average surface concentration of electroactive NiFe catalyst in the film was found to be ∼9.6 × 10 mol cm , implying that a surprisingly high fraction (37%) of NiFe units incorporated in the MOF are electroactive. By cyclic voltammetry, we showed that NiFe maintains its electrocatalytic capabilities for H reduction inside the MOF cavities, even if under controlled-potential electrolysis conditions the activity of NiFe cannot be discerned from that of free PCN-777 and FTO.
[Mh] Termos MeSH primário: Materiais Biomiméticos/química
Hidrogenase/química
Ferro/química
Estruturas Metalorgânicas/química
Níquel/química
Zircônio/química
[Mh] Termos MeSH secundário: Catálise
Técnicas Eletroquímicas
Modelos Moleculares
Oxirredução
Prótons
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metal-Organic Frameworks); 0 (Protons); 7OV03QG267 (Nickel); C6V6S92N3C (Zirconium); E1UOL152H7 (Iron); EC 1.12.- (nickel-iron hydrogenase); EC 1.12.7.2 (Hydrogenase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1021/acs.inorgchem.7b01824


  2 / 6374 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29188999
[Au] Autor:Song LC; Zhu L; Hu FQ; Wang YX
[Ad] Endereço:Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, College of Chemistry, and ‡Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, China.
[Ti] Título:Studies on Chemical Reactivity and Electrocatalysis of Two Acylmethyl(hydroxymethyl)pyridine Ligand-Containing [Fe]-Hydrogenase Models (2-COCH -6-HOCH C H N)Fe(CO) L (L = η -SCOMe, η -2-SC H N).
[So] Source:Inorg Chem;56(24):15216-15230, 2017 Dec 18.
[Is] ISSN:1520-510X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:On the basis of preparation and characterization of [Fe]-H ase models (2-COCH -6-HOCH C H N)Fe(CO) L (A, L = η -SCOMe; B, L = η -2-SC H N), the chemical reactivities of A and B with various electrophilic and nucleophilic reagents have been investigated, systematically. Thus, when A reacted with 1 equiv of MeCOCl in the presence of Et N in MeCN to give the η -SCOMe-coordinated acylation product (2-COCH -6-MeCO CH C H N)Fe(CO) (η -SCOMe) (1), treatment of A with excess HBF ·Et O in MeCN gave the cationic MeCN-coordinated complex [(2-COCH -6-HOCH C H N)Fe(CO) (MeCN)](BF ) (2). In addition, when 2 was treated with 1 equiv of 2,6-(p-4-MeC H ) C H SK or PPh in CH Cl to give the thiophenolato- and PPh -substituted derivatives (2-COCH -6-HOCH C H N)Fe(CO) [2,6-(p-MeC H ) C H S] (3) and [(2-COCH -6-HOCH C H N)Fe(CO) (PPh )](BF ) (4), treatment of B with 1 equiv of PMe or P(OMe) in THF afforded the phosphine- and phosphite-substituted complexes (2-COCH -6-HOCH C H N)(η -2-SC H N)Fe(CO) L (5, L = PMe ; 6, L = P(OMe) ). Interestingly, in contrast to A, when B reacted with excess HBF ·Et O in MeCN to afford the BF adduct [2-COCH -6-HO(BF )CH C H N]Fe(CO) (η -2-SC H N) (7), reaction of B with 1 equiv of p-MeC H COCl in the presence of Et N in MeCN gave not only the expected 2-acylmethyl-6-p-toluoyloxomethylpyridine-containing complex (2-COCH -6-p-MeC H CO CH C H N)Fe(CO) (η -2-SC H N) (8), but also gave the unexpected 2-toluoyloxovinyl-6-toluoyloxomethylpyridine-containing complex (2-p-MeC H CO C H-6-p-MeC H CO CH C H N)Fe(CO) (η -2-SC H N) (9). While the possible pathways for the novel reactions leading to complexes 1, 2, and 7-9 are suggested, the structures of complexes B, 1-4, and 6-9 were unambiguously confirmed by X-ray crystallography. In addition, model complexes A and B have been found to be catalysts for proton reduction to H from TFA under CV conditions.
[Mh] Termos MeSH primário: Materiais Biomiméticos/química
Hidrogenase/química
Compostos de Ferro/química
Proteínas com Ferro-Enxofre/química
Piridinas/química
[Mh] Termos MeSH secundário: Catálise
Cristalografia por Raios X
Técnicas Eletroquímicas
Ligantes
Modelos Moleculares
Oxirredução
Prótons
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Iron Compounds); 0 (Iron-Sulfur Proteins); 0 (Ligands); 0 (Protons); 0 (Pyridines); 0 (acylmethyl(hydroxymethyl)pyridine); EC 1.12.- (iron hydrogenase); EC 1.12.7.2 (Hydrogenase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1021/acs.inorgchem.7b02582


  3 / 6374 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28453953
[Au] Autor:Ramaraju H; Miller SJ; Kohn DH
[Ad] Endereço:Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
[Ti] Título:Dual-functioning peptides discovered by phage display increase the magnitude and specificity of BMSC attachment to mineralized biomaterials.
[So] Source:Biomaterials;134:1-12, 2017 Jul.
[Is] ISSN:1878-5905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Design of biomaterials for cell-based therapies requires presentation of specific physical and chemical cues to cells, analogous to cues provided by native extracellular matrices (ECM). We previously identified a peptide sequence with high affinity towards apatite (VTKHLNQISQSY, VTK) using phage display. The aims of this study were to identify a human MSC-specific peptide sequence through phage display, combine it with the apatite-specific sequence, and verify the specificity of the combined dual-functioning peptide to both apatite and human bone marrow stromal cells. In this study, a combinatorial phage display identified the cell binding sequence (DPIYALSWSGMA, DPI) which was combined with the mineral binding sequence to generate the dual peptide DPI-VTK. DPI-VTK demonstrated significantly greater binding affinity (1/K ) to apatite surfaces compared to VTK, phosphorylated VTK (VTK ), DPI-VTK , RGD-VTK, and peptide-free apatite surfaces (p < 0.01), while significantly increasing hBMSC adhesion strength (τ , p < 0.01). MSCs demonstrated significantly greater adhesion strength to DPI-VTK compared to other cell types, while attachment of MC3T3 pre-osteoblasts and murine fibroblasts was limited (p < 0.01). MSCs on DPI-VTK coated surfaces also demonstrated increased spreading compared to pre-osteoblasts and fibroblasts. MSCs cultured on DPI-VTK coated apatite films exhibited significantly greater proliferation compared to controls (p < 0.001). Moreover, early and late stage osteogenic differentiation markers were elevated on DPI-VTK coated apatite films compared to controls. Taken together, phage display can identify non-obvious cell and material specific peptides to increase human MSC adhesion strength to specific biomaterial surfaces and subsequently increase cell proliferation and differentiation. These new peptides expand biomaterial design methodology for cell-based regeneration of bone defects. This strategy of combining cell and material binding phage display derived peptides is broadly applicable to a variety of systems requiring targeted adhesion of specific cell populations, and may be generalized to the engineering of any adhesion surface.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Células Mesenquimais Estromais/citologia
Peptídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Materiais Biomiméticos/química
Adesão Celular/efeitos dos fármacos
Adesão Celular/fisiologia
Diferenciação Celular/efeitos dos fármacos
Diferenciação Celular/fisiologia
Células Cultivadas
Fibroblastos/citologia
Fibroblastos/efeitos dos fármacos
Fibroblastos/fisiologia
Seres Humanos
Células Mesenquimais Estromais/efeitos dos fármacos
Células Mesenquimais Estromais/fisiologia
Osteoblastos/citologia
Osteoblastos/efeitos dos fármacos
Osteoblastos/fisiologia
Peptídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Peptides)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  4 / 6374 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28471002
[Au] Autor:Cecchini MM; Reale S; Manini P; d'Ischia M; De Angelis F
[Ad] Endereço:Department of Physical and Chemical Sciences, University of L'Aquila, Via Vetoio, Coppito, L'Aquila, Italy.
[Ti] Título:Modeling Fungal Melanin Buildup: Biomimetic Polymerization of 1,8-Dihydroxynaphthalene Mapped by Mass Spectrometry.
[So] Source:Chemistry;23(33):8092-8098, 2017 Jun 12.
[Is] ISSN:1521-3765
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Due to the emerging biomedical relevance and technological potential of fungal melanins, and prompted by the virtual lack of information about their structural arrangement, an optimized synthetic protocol has been devised for a potential structural model of Ascomyces allomelanin through enzyme-catalyzed oxidative polymerization of 1,8-dihydroxynaphthalene (1,8-DHN). Electrospray ionization mass spectrometry (ESI-MS) measurements of freshly synthesized DHN-polymer recorded in the negative ion mode allowed detection of oligomers up to m/z 4000, separated by 158 Da, corresponding to the in-chain DHN-unit. The dominant peaks were assigned to singly-charged distribution, up to 23 repeating units, whereas a doubly charged polymer distribution was also detectable. Chemical derivatization, ultra-performance liquid chromatography (UPLC)-ESI MS, and MS/MS data confirmed that oxidative polymerization of 1,8-DHN proceeds through C-C coupling of the naphthalene rings. The new insights reported here into synthetic 1,8-DHN oligomers/polymers as a mimic of fungal melanins may guide novel interesting advances and applications in the field of biomimetic functional materials.
[Mh] Termos MeSH primário: Materiais Biomiméticos/química
Proteínas Fúngicas/metabolismo
Fungos/metabolismo
Melaninas/metabolismo
Naftóis/química
[Mh] Termos MeSH secundário: Biocatálise
Materiais Biomiméticos/metabolismo
Cromatografia Líquida de Alta Pressão
Proteínas Fúngicas/química
Peroxidase do Rábano Silvestre/metabolismo
Melaninas/química
Oxirredução
Polimerização
Espectrometria de Massas por Ionização por Electrospray
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Melanins); 0 (Naphthols); 569-42-6 (1,8-dihydroxynaphthalene); EC 1.11.1.- (Horseradish Peroxidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1002/chem.201701951


  5 / 6374 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29395078
[Au] Autor:Han S; Lee JY; Heo EY; Kwon IK; Yune TY; Youn I
[Ad] Endereço:Biomedical Research Institute, Korea Institute of Science and Technology, 5, Hwarang-ro 14-gil, Seongbuk-gu, Seoul, 02791, Republic of Korea.
[Ti] Título:Implantation of a Matrigel-loaded agarose scaffold promotes functional regeneration of axons after spinal cord injury in rat.
[So] Source:Biochem Biophys Res Commun;496(3):785-791, 2018 02 12.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An agarose scaffold can be useful for supporting and guiding injured axons after spinal cord injury (SCI), but the electrophysiological signal of regenerated axon in scaffolds has not yet been determined. The current study investigated whether a Matrigel-loaded agarose scaffold would enhance the regeneration of axons after SCI. Moreover, the functional connectivity of regenerated axons within the channels of the scaffold was evaluated by directly recording motor evoked potentials. Our data showed that the agarose scaffold containing Matrigel can support and enhance linearly organized axon regeneration after SCI. Additionally, motor evoked potentials were successfully recorded from regenerated axons. These results demonstrate that an agarose scaffold loaded with Matrigel could promote the regeneration of axons and guide the reconnection of functional axons after SCI.
[Mh] Termos MeSH primário: Axônios/patologia
Colágeno/química
Regeneração Tecidual Guiada/instrumentação
Laminina/química
Regeneração Nervosa/fisiologia
Proteoglicanas/química
Traumatismos da Medula Espinal/patologia
Traumatismos da Medula Espinal/terapia
Tecidos Suporte
[Mh] Termos MeSH secundário: Animais
Materiais Biomiméticos/síntese química
Combinação de Medicamentos
Desenho de Equipamento
Análise de Falha de Equipamento
Masculino
Crescimento Neuronal
Próteses e Implantes
Ratos
Ratos Sprague-Dawley
Recuperação de Função Fisiológica
Sefarose/química
Traumatismos da Medula Espinal/fisiopatologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drug Combinations); 0 (Laminin); 0 (Proteoglycans); 119978-18-6 (matrigel); 9007-34-5 (Collagen); 9012-36-6 (Sepharose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180204
[St] Status:MEDLINE


  6 / 6374 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29358689
[Au] Autor:Gueye M; Manathunga M; Agathangelou D; Orozco Y; Paolino M; Fusi S; Haacke S; Olivucci M; Léonard J
[Ad] Endereço:Université de Strasbourg, CNRS, Institut de Physique et Chimie des Matériaux de Strasbourg, UMR 7504, F-67034, Strasbourg, France.
[Ti] Título:Engineering the vibrational coherence of vision into a synthetic molecular device.
[So] Source:Nat Commun;9(1):313, 2018 01 22.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The light-induced double-bond isomerization of the visual pigment rhodopsin operates a molecular-level optomechanical energy transduction, which triggers a crucial protein structure change. In fact, rhodopsin isomerization occurs according to a unique, ultrafast mechanism that preserves mode-specific vibrational coherence all the way from the reactant excited state to the primary photoproduct ground state. The engineering of such an energy-funnelling function in synthetic compounds would pave the way towards biomimetic molecular machines capable of achieving optimum light-to-mechanical energy conversion. Here we use resonance and off-resonance vibrational coherence spectroscopy to demonstrate that a rhodopsin-like isomerization operates in a biomimetic molecular switch in solution. Furthermore, by using quantum chemical simulations, we show why the observed coherent nuclear motion critically depends on minor chemical modifications capable to induce specific geometric and electronic effects. This finding provides a strategy for engineering vibrationally coherent motions in other synthetic systems.
[Mh] Termos MeSH primário: Materiais Biomiméticos/química
Indanos/química
Dispositivos Ópticos
Pirróis/química
Retinaldeído/química
Rodopsina/química
[Mh] Termos MeSH secundário: Alquilação
Animais
Materiais Biomiméticos/síntese química
Engenharia Química
Seres Humanos
Indanos/síntese química
Luz
Processos Fotoquímicos
Pirróis/síntese química
Teoria Quântica
Análise Espectral/instrumentação
Análise Espectral/métodos
Vibração
Visão Ocular/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Indans); 0 (Pyrroles); 9009-81-8 (Rhodopsin); RR725D715M (Retinaldehyde)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02668-w


  7 / 6374 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29253563
[Au] Autor:Povarova NV; Markina NM; Baranov MS; Barinov NA; Klinov DV; Kozhemyako VB; Lukyanov KA
[Ad] Endereço:Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia; Nizhny Novgorod State Medical Academy, Minin and Pozharsky Sq. 10/1, 603005 Nizhny Novgorod, Russia. Electronic address: povarovanv@gmail.com.
[Ti] Título:A water-soluble precursor for efficient silica polymerization by silicateins.
[So] Source:Biochem Biophys Res Commun;495(2):2066-2070, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Silicateins, the spicule-forming proteins from marine demosponges capable to polymerize silica, are popular objects of biomineralization studies due to their ability to form particles varied in shape and composition under physiological conditions. Despite the occurrence of the many approaches to nanomaterial synthesis using silicateins, biochemical properties of this protein family are poorly characterized. The main reason for this is that tetraethyl orthosilicate (TEOS), the commonly used silica acid precursor, is almost insoluble in water and thus is poorly available for the protein. To solve this problem, we synthesized new water-soluble silica precursor, tetra(glycerol)orthosilicate (TGS), and characterized biochemical properties of the silicatein A1 from marine sponge Latrunculia oparinae. Compared to TEOS, TGS ensured much greater activity of silicatein and was less toxic for the mammalian cell culture. We evaluated optimum conditions for the enzyme - pH range, temperature and TGS concentration. We concluded that TGS is a useful silica acid precursor that can be used for silica particles synthesis and in vivo applications.
[Mh] Termos MeSH primário: Materiais Biomiméticos/síntese química
Catepsinas/química
Polímeros/síntese química
Poríferos/química
Dióxido de Silício/síntese química
Água/química
[Mh] Termos MeSH secundário: Animais
Teste de Materiais
Solubilidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Polymers); 059QF0KO0R (Water); 7631-86-9 (Silicon Dioxide); EC 3.4.- (Cathepsins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


  8 / 6374 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29330415
[Au] Autor:Zhou Y; Damasceno PF; Somashekar BS; Engel M; Tian F; Zhu J; Huang R; Johnson K; McIntyre C; Sun K; Yang M; Green PF; Ramamoorthy A; Glotzer SC; Kotov NA
[Ad] Endereço:School of Biomedical Engineering, School of Ophthalmology and Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325011, China. zhouyl@wibe.ac.cn.
[Ti] Título:Unusual multiscale mechanics of biomimetic nanoparticle hydrogels.
[So] Source:Nat Commun;9(1):181, 2018 01 12.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Viscoelastic properties are central for gels and other materials. Simultaneously, high storage and loss moduli are difficult to attain due to their contrarian requirements to chemical structure. Biomimetic inorganic nanoparticles offer a promising toolbox for multiscale engineering of gel mechanics, but a conceptual framework for their molecular, nanoscale, mesoscale, and microscale engineering as viscoelastic materials is absent. Here we show nanoparticle gels with simultaneously high storage and loss moduli from CdTe nanoparticles. Viscoelastic figure of merit reaches 1.83 MPa exceeding that of comparable gels by 100-1000 times for glutathione-stabilized nanoparticles. The gels made from the smallest nanoparticles display the highest stiffness, which was attributed to the drastic change of GSH configurations when nanoparticles decrease in size. A computational model accounting for the difference in nanoparticle interactions for variable GSH configurations describes the unusual trends of nanoparticle gel viscoelasticity. These observations are generalizable to other NP gels interconnected by supramolecular interactions and lead to materials with high-load bearing abilities and energy dissipation needed for multiple technologies.
[Mh] Termos MeSH primário: Hidrogéis/síntese química
Nanopartículas/química
[Mh] Termos MeSH secundário: Materiais Biomiméticos
Compostos de Cádmio/química
Glutationa/química
Fenômenos Mecânicos
Telúrio/química
Substâncias Viscoelásticas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cadmium Compounds); 0 (Hydrogels); 0 (Viscoelastic Substances); GAN16C9B8O (Glutathione); NQA0O090ZJ (Tellurium); STG188WO13 (cadmium telluride)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180114
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02579-w


  9 / 6374 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29236205
[Au] Autor:Chiti MC; Dolmans MM; Mortiaux L; Zhuge F; Ouni E; Shahri PAK; Van Ruymbeke E; Champagne SD; Donnez J; Amorim CA
[Ad] Endereço:Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Avenue Mounier 52, bte. B1.52.02, 1200, Brussels, Belgium.
[Ti] Título:A novel fibrin-based artificial ovary prototype resembling human ovarian tissue in terms of architecture and rigidity.
[So] Source:J Assist Reprod Genet;35(1):41-48, 2018 Jan.
[Is] ISSN:1573-7330
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The aim of this study is to optimize fibrin matrix composition in order to mimic human ovarian tissue architecture for human ovarian follicle encapsulation and grafting. METHODS: Ultrastructure of fresh human ovarian cortex in age-related women (n = 3) and different fibrin formulations (F12.5/T1, F30/T50, F50/T50, F75/T75), rheology of fibrin matrices and histology of isolated and encapsulated human ovarian follicles in these matrices. RESULTS: Fresh human ovarian cortex showed a highly fibrous and structurally inhomogeneous architecture in three age-related patients, but the mean ± SD of fiber thickness (61.3 to 72.4 nm) was comparable between patients. When the fiber thickness of four different fibrin formulations was compared with human ovarian cortex, F50/T50 and F75/T75 showed similar fiber diameters to native tissue, while F12.5/T1 was significantly different (p value < 0.01). In addition, increased concentrations of fibrin exhibited enhanced storage modulus with F50/T50, resembling physiological ovarian rigidity. Excluding F12.5/T1 from further analysis, only three remaining fibrin matrices (F30/T50, F50/T50, F75/T75) were histologically investigated. For this, frozen-thawed fragments of human ovarian tissue collected from 22 patients were used to isolate ovarian follicles and encapsulate them in the three fibrin formulations. All three yielded similar follicle recovery and loss rates soon after encapsulation. Therefore, based on fiber thickness, porosity, and rigidity, we selected F50/T50 as the fibrin formulation that best mimics native tissue. CONCLUSIONS: Of all the different fibrin matrix concentrations tested, F50/T50 emerged as the combination of choice in terms of ultrastructure and rigidity, most closely resembling human ovarian cortex.
[Mh] Termos MeSH primário: Órgãos Artificiais
Fibrina/química
Ovário
[Mh] Termos MeSH secundário: Materiais Biomiméticos/química
Composição de Medicamentos
Elasticidade
Feminino
Dureza
Seres Humanos
Fenômenos Mecânicos
Folículo Ovariano/transplante
Folículo Ovariano/ultraestrutura
Ovário/química
Ovário/citologia
Ovário/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9001-31-4 (Fibrin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1007/s10815-017-1091-3


  10 / 6374 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29298982
[Au] Autor:Li X; Zhai T; Gao P; Cheng H; Hou R; Lou X; Xia F
[Ad] Endereço:State Key Laboratory of Material Processing and Die & Mould Technology, School of Material Sciences and Engineering, Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), 430074, Wu
[Ti] Título:Role of outer surface probes for regulating ion gating of nanochannels.
[So] Source:Nat Commun;9(1):40, 2018 01 03.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nanochannels with functional elements have shown promise for DNA sequencing, single-molecule sensing, and ion gating. Ionic current measurement is currently a benchmark, but is focused solely on the contribution from nanochannels' inner-wall functional elements (NIWFE); the attributes of functional elements at nanochannels' outer surface (NOSFE) are nearly ignored, and remain elusive. Here we show that the role of NOSFE and NIWFE for ion gating can be distinguished by constructing DNA architectures using dual-current readout. The established molecular switches have continuously tunable and reversible ion-gating ability. We find that NOSFE exhibits negligible ion-gating behavior, but it can produce a synergistic effect in alliance with NIWFE. Moreover, the high-efficiency gating systems display more noticeable synergistic effect than the low-efficiency ones. We also reveal that the probe amount of NOSFE and NIWFE is almost equally distributed in our biomimetic nanochannels, which is potentially a premise for the synergistic ion-gating phenomena.
[Mh] Termos MeSH primário: Materiais Biomiméticos
Ativação do Canal Iônico
Transporte de Íons
Nanoestruturas
[Mh] Termos MeSH secundário: Óxido de Alumínio
Técnicas de Patch-Clamp
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
LMI26O6933 (Aluminum Oxide)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180105
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02447-7



página 1 de 638 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde