Base de dados : MEDLINE
Pesquisa : J01.897.280.500.545 [Categoria DeCS]
Referências encontradas : 15749 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 1575 ir para página                         

  1 / 15749 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28699512
[Au] Autor:Vineeth Kumar TV; Sanil G
[Ad] Endereço:Molecular Ecology Laboratory, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala. India.
[Ti] Título:A Review of the Mechanism of Action of Amphibian Antimicrobial Peptides Focusing on Peptide-Membrane Interaction and Membrane Curvature.
[So] Source:Curr Protein Pept Sci;18(12):1263-1272, 2017.
[Is] ISSN:1875-5550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Research interests on amphibian antimicrobial peptides (AMPs) are currently increasing because of their capability to combat microorganisms from both terrestrial and aquatic environments, which are the warehouses of human pathogens. The most remarkable feature of AMPs are their mechanism of action, primarily targeted to anionic membranes. Researchers have postulated many models to describe peptide- membrane interaction, which leads to membrane permeation/intracellular targeting. Despite these models information regarding the relationship between membrane curvature and peptidemembrane interaction is scarce. This relationship could be clearly depicted using the two-state model and interfacial activity model. In the review, we discuss in detail the two state and interfacial activity models and explain the influence of membrane curvature on peptide binding and the membrane interaction of curvature-sensitive peptides. In addition, the models proposed to explain the mechanism of action of membrane lytic and non-lytic AMPs are also reviewed.
[Mh] Termos MeSH primário: Anti-Infecciosos/química
Peptídeos Catiônicos Antimicrobianos/química
Membrana Celular/química
Bicamadas Lipídicas/química
Modelos Estruturais
[Mh] Termos MeSH secundário: Anfíbios/metabolismo
Animais
Anti-Infecciosos/isolamento & purificação
Anti-Infecciosos/farmacologia
Peptídeos Catiônicos Antimicrobianos/isolamento & purificação
Peptídeos Catiônicos Antimicrobianos/farmacologia
Membrana Celular/efeitos dos fármacos
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Eletricidade Estática
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antimicrobial Cationic Peptides); 0 (Lipid Bilayers)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.2174/1389203718666170710114932


  2 / 15749 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28381544
[Au] Autor:Li R; Ding C; Zhang J; Xie M; Park D; Ding Y; Chen G; Zhang G; Gilbert-Ross M; Zhou W; Marcus AI; Sun SY; Chen ZG; Sica GL; Ramalingam SS; Magis AT; Fu H; Khuri FR; Curran WJ; Owonikoko TK; Shin DM; Zhou J; Deng X
[Ad] Endereço:Department of Radiation Oncology, Emory University School of Medicine and Winship Cancer Institute of Emory University, Atlanta, Georgia.
[Ti] Título:Modulation of Bax and mTOR for Cancer Therapeutics.
[So] Source:Cancer Res;77(11):3001-3012, 2017 Jun 01.
[Is] ISSN:1538-7445
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A rationale exists for pharmacologic manipulation of the serine (S)184 phosphorylation site of the proapoptotic Bcl2 family member Bax as an anticancer strategy. Here, we report the refinement of the Bax agonist SMBA1 to generate CYD-2-11, which has characteristics of a suitable clinical lead compound. CYD-2-11 targeted the structural pocket proximal to S184 in the C-terminal region of Bax, directly activating its proapoptotic activity by inducing a conformational change enabling formation of Bax homooligomers in mitochondrial membranes. In murine models of small-cell and non-small cell lung cancers, including patient-derived xenograft and the genetically engineered mutant KRAS-driven lung cancer models, CYD-2-11 suppressed malignant growth without evident significant toxicity to normal tissues. In lung cancer patients treated with mTOR inhibitor RAD001, we observed enhanced S184 Bax phosphorylation in lung cancer cells and tissues that inactivates the propaoptotic function of Bax, contributing to rapalog resistance. Combined treatment of CYD-2-11 and RAD001 in murine lung cancer models displayed strong synergistic activity and overcame rapalog resistance and Taken together, our findings provide preclinical evidence for a pharmacologic combination of Bax activation and mTOR inhibition as a rational strategy to improve lung cancer treatment. .
[Mh] Termos MeSH primário: Neoplasias Pulmonares/genética
Serina-Treonina Quinases TOR/genética
Serina-Treonina Quinases TOR/metabolismo
Proteína X Associada a bcl-2/genética
Proteína X Associada a bcl-2/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Proliferação Celular
Modelos Animais de Doenças
Seres Humanos
Neoplasias Pulmonares/patologia
Camundongos
Modelos Estruturais
Fosforilação
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (bcl-2-Associated X Protein); EC 2.7.1.1 (MTOR protein, human); EC 2.7.1.1 (TOR Serine-Threonine Kinases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1158/0008-5472.CAN-16-2356


  3 / 15749 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28327439
[Au] Autor:Chávez-Fumagalli MA; Schneider MS; Lage DP; Machado-de-Ávila RA; Coelho EA
[Ad] Endereço:Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: miguel.fumagalli@pq.cnpq.br.
[Ti] Título:An in silico functional annotation and screening of potential drug targets derived from Leishmania spp. hypothetical proteins identified by immunoproteomics.
[So] Source:Exp Parasitol;176:66-74, 2017 May.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Leishmaniasis is a parasitic disease caused by the protozoan of the Leishmania genus. While no human vaccine is available, drugs such as pentavalent antimonials, pentamidine and amphotericin B are used for treat the patients. However, the high toxicity of these pharmaceutics, the emergence of parasite resistance and/or their high cost have showed to the urgent need of identify new targets to be employed in the improvement of the treatment against leishmaniasis. In a recent immunoproteomics approach performed in the Leishmania infantum species, 104 antigenic proteins were recognized by antibodies in sera of visceral leishmaniasis (VL) dogs. Some of them were later showed to be effective diagnostic markers and/or vaccine candidates against the disease. Between these proteins, 24 considered as hypothetical were identified in the promastigote and amastigote-like extracts of the parasites. The present study aimed to use bioinformatics tools to select new drug targets between these hypothetical proteins. Their cellular localization was predicted to be seven membrane proteins, as well as eight cytoplasmic, three nuclear, one mitochondrial and five proteins remained unclassified. Their functions were predicted as being two transport proteins, as well as five with metabolic activity, three as cell signaling and fourteen proteins remained unclassified. Ten hypothetical proteins were well-annotated and compared to their homology regarding to human proteins. Two proteins, a calpain-like and clavaminate synthase-like proteins were selected by using Docking analysis as being possible drug targets. In this sense, the present study showed the employ of new strategies to select possible drug candidates, according their localization and biological function in Leishmania parasites, aiming to treat against VL.
[Mh] Termos MeSH primário: Biologia Computacional/métodos
Leishmania infantum/efeitos dos fármacos
Proteômica/métodos
Proteínas de Protozoários/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Calpaína/química
Calpaína/efeitos dos fármacos
Calpaína/imunologia
Sistemas de Liberação de Medicamentos
Seres Humanos
Leishmania infantum/química
Leishmania infantum/imunologia
Leishmaniose Visceral/tratamento farmacológico
Oxigenases de Função Mista/química
Oxigenases de Função Mista/efeitos dos fármacos
Oxigenases de Função Mista/imunologia
Modelos Estruturais
Conformação Molecular
Proteínas de Protozoários/química
Proteínas de Protozoários/efeitos dos fármacos
Curva ROC
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protozoan Proteins); EC 1.- (Mixed Function Oxygenases); EC 1.14.11.- (clavaminate synthase); EC 3.4.22.- (Calpain)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE


  4 / 15749 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28321577
[Au] Autor:Richard Strimbeck G
[Ad] Endereço:Department of Biology, Norwegian University of Science and Technology, 7491, Trondheim, Norway. richard.strimbeck@ntnu.no.
[Ti] Título:Hiding in plain sight: the F segment and other conserved features of seed plant SK dehydrins.
[So] Source:Planta;245(5):1061-1066, 2017 May.
[Is] ISSN:1432-2048
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:MAIN CONCLUSION: An 11-residue amino acid sequence, DRGLFDFLGKK, is highly conserved in a subset of dehydrins found across the full spectrum of seed plants and here given the name F-segment. An 11-residue amino acid sequence, DRGLFDFLGKK, is highly conserved in identity and polarity in 130 non-redundant dehydrin sequences representing conifers and all major angiosperm groups. This newly described motif is here given the name F segment based on the pair of hydrophobic F residues at the core of the sequence. The majority of dehydrins previously classified as SK dehydrins contain one F segment N terminal to the S and K segments and can accordingly be reclassified as FSK dehydrins. A cysteine-containing variant, GCGMFDFLKK, occurs in a few rosid and asterid taxa. The S segment in this and other dehydrin types also includes previously overlooked conserved features, including a KLHR prefix and charged or G residues within and following the characteristic string of S residues. Secondary structure prediction models indicate that the F segment and S segment prefix may form amphipathic helices that could be involved in membrane or protein binding.
[Mh] Termos MeSH primário: Coniferophyta/química
Magnoliopsida/química
Proteínas de Plantas/química
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Sequência de Aminoácidos
Coniferophyta/genética
Sequência Conservada
Magnoliopsida/genética
Modelos Estruturais
Proteínas de Plantas/genética
Estrutura Terciária de Proteína
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 134711-03-8 (dehydrin proteins, plant)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1007/s00425-017-2679-7


  5 / 15749 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28159683
[Au] Autor:Davidson N; Tong HJ; Kalberer M; Seville PC; Ward AD; Kuimova MK; Pope FD
[Ad] Endereço:School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
[Ti] Título:Measurement of the Raman spectra and hygroscopicity of four pharmaceutical aerosols as they travel from pressurised metered dose inhalers (pMDI) to a model lung.
[So] Source:Int J Pharm;520(1-2):59-69, 2017 Mar 30.
[Is] ISSN:1873-3476
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Particle inhalation is an effective and rapid delivery method for a variety of pharmaceuticals, particularly bronchodilation drugs used for treating asthma and COPD. Conditions of relative humidity and temperature inside the lungs are generally very different from the outside ambient air, with the lung typically being warmer and more humid. Changes in humidity, from inhaler to lung, can cause hygroscopic phase transitions and particle growth. Increasing particle size and mass can negatively affect particle deposition within the lung leading to inefficient treatment, while deliquescence prior to impaction is liable to accelerate drug uptake. To better understand the hygroscopic properties of four pharmaceutical aerosol particles; pharmaceutical particles from four commercially available pressurised metered dose inhalers (pMDIs) were stably captured in an optical trap, and their composition was examined online via Raman spectroscopy. Micron-sized particles of salbutamol sulfate, salmeterol xinafoate, fluticasone propionate and ciclesonide were levitated and examined over a range of relative humidity values inside a chamber designed to mimic conditions within the respiratory tract. The effect of temperature upon hygroscopicity was also investigated for salbutamol sulfate particles. Salbutamol sulfate was found to have significant hygroscopicity, salmeterol xinafoate showed some hygroscopic interactions, whilst fluticasone propionate and ciclesonide revealed no observable hygroscopicity. Thermodynamic and structural modelling is used to explain the observed experimental results.
[Mh] Termos MeSH primário: Aerossóis/química
Análise Espectral Raman
Molhabilidade
[Mh] Termos MeSH secundário: Albuterol/química
Fluticasona/química
Umidade
Inaladores Dosimetrados
Modelos Estruturais
Tamanho da Partícula
Pregnenodionas/química
Xinafoato de Salmeterol/química
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aerosols); 0 (Pregnenediones); 6EW8Q962A5 (Salmeterol Xinafoate); CUT2W21N7U (Fluticasone); QF8SVZ843E (Albuterol); S59502J185 (ciclesonide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE


  6 / 15749 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27999439
[Au] Autor:Eguchi Y; Okajima T; Tochio N; Inukai Y; Shimizu R; Ueda S; Shinya S; Kigawa T; Fukamizo T; Igarashi M; Utsumi R
[Ad] Endereço:Department of Bioscience, Graduate School of Agriculture, Kindai University, Nara, Japan.
[Ti] Título:Angucycline antibiotic waldiomycin recognizes common structural motif conserved in bacterial histidine kinases.
[So] Source:J Antibiot (Tokyo);70(3):251-258, 2017 Mar.
[Is] ISSN:0021-8820
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Two-component signal transduction systems (TCSs), composed of a histidine kinase sensor (HK) and its cognate response regulator, sense and respond to environmental changes and are related to the virulence of pathogens. TCSs are potential targets for alternative antibiotics and anti-virulence agents. Here we found that waldiomycin, an angucycline antibiotic that inhibits a growth essential HK, WalK, in Gram-positive bacteria, also inhibits several class I HKs from the Gram-negative Escherichia coli. NMR analyses and site-directed mutagenesis studies using the osmo-sensing EnvZ, a prototypical HK of E. coli, showed that waldiomycin directly binds to both H-box and X-region, which are the two conserved regions in the dimerization-inducing and histidine-containing phosphotransfer (DHp) domain of HKs. Waldiomycin inhibits phosphorylation of the conserved histidine in the H-box. Analysis of waldiomycin derivatives suggests that the angucyclic ring, situated near the H-box in the waldiomycin-EnvZ DHp domain complex model, is responsible for the inhibitory activity. We demonstrate that waldiomycin is an HK inhibitor binding to the H-box region and has the potential of inhibiting a broad spectrum of HKs.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Histidina Quinase/antagonistas & inibidores
Histidina Quinase/química
Quinonas/farmacologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas da Membrana Bacteriana Externa/antagonistas & inibidores
Proteínas da Membrana Bacteriana Externa/efeitos dos fármacos
Proteínas da Membrana Bacteriana Externa/genética
Sequência Conservada
Escherichia coli/efeitos dos fármacos
Escherichia coli/enzimologia
Proteínas de Escherichia coli/antagonistas & inibidores
Proteínas de Escherichia coli/efeitos dos fármacos
Proteínas de Escherichia coli/genética
Histidina Quinase/genética
Modelos Estruturais
Complexos Multienzimáticos/antagonistas & inibidores
Complexos Multienzimáticos/efeitos dos fármacos
Complexos Multienzimáticos/genética
Mutagênese Sítio-Dirigida
Fosforilação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Outer Membrane Proteins); 0 (Escherichia coli Proteins); 0 (Multienzyme Complexes); 0 (Quinones); 0 (waldiomycin); EC 2.7.13.1 (Histidine Kinase); EC 2.7.3.- (envZ protein, E coli)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE
[do] DOI:10.1038/ja.2016.151


  7 / 15749 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27641734
[Au] Autor:Yang LY; Liu XF; Yang Y; Yang LL; Liu KW; Tang YB; Zhang M; Tan MJ; Cheng SM; Xu YC; Yang HY; Liu ZJ; Song GJ; Huang W
[Ad] Endereço:CAS Key Laboratory of Receptor Research, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
[Ti] Título:Biochemical features of the adhesion G protein-coupled receptor CD97 related to its auto-proteolysis and HeLa cell attachment activities.
[So] Source:Acta Pharmacol Sin;38(1):56-68, 2017 Jan.
[Is] ISSN:1745-7254
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:CD97 belongs to the adhesion GPCR family characterized by a long ECD linked to the 7TM via a GPCR proteolytic site (GPS) and plays important roles in modulating cell migration and invasion. CD97 (EGF1-5) is a splicing variant of CD97 that recognizes a specific ligand chondroitin sulfate on cell membranes and the extracellular matrix. The aim of this study was to elucidate the extracellular molecular basis of the CD97 EGF1-5 isoform in protein expression, auto-proteolysis and cell adhesion, including epidermal growth factor (EGF)-like domain, GPCR autoproteolysis-inducing (GAIN) domain, as well as GPS mutagenesis and N-glycosylation. Both wild-type (WT) CD97-ECD and its truncated, GPS mutated, PNGase F-deglycosylated, and N-glycosylation site mutated forms were expressed and purified. The auto-proteolysis of the proteins was analyzed with Western blotting and SDS-PAGE. Small angle X-ray scattering (SAXS) and molecular modeling were used to determine a structural profile of the properly expressed receptor. Potential N-glycosylation sites were identified using MS and were modulated with PNGase F digestion and glyco-site mutations. A flow cytometry-based HeLa cell attachment assay was used for all aforementioned CD97 variants to elucidate the molecular basis of CD97-HeLa interactions. A unique concentration-dependent GPS auto-proteolysis was observed in CD97 EGF1-5 isoform with the highest concentration (4 mg/mL) per sample was self-cleaved much faster than the lower concentration (0.1 mg/mL), supporting an intermolecular mechanism of auto-proteolysis that is distinct to the reported intramolecular mechanism for other CD97 isoforms. N-glycosylation affected the auto-proteolysis of CD97 EGF1-5 isoform in a similar way as the other previously reported CD97 isoforms. SAXS data for WT and deglycosylated CD97ECD revealed a spatula-like shape with GAIN and EGF domains constituting the body and handle, respectively. Structural modeling indicated a potential interaction between the GAIN and EGF5 domains accounting for the absence of expression of the GAIN domain itself, although EGF5-GAIN was expressed similarly in the wild-type protein. For HeLa cell adhesion, the GAIN-truncated forms showed dramatically reduced binding affinity. The PNGase F-deglycosylated and GPS mutated forms also exhibited reduced HeLa attachment compared with WT CD97. However, neither N-glycosylation mutagenesis nor auto-proteolysis inhibition caused by N-glycosylation mutagenesis affected CD97-HeLa cell interactions. A comparison of the HeLa binding affinities of PNGase F-digested, GPS-mutated and N-glycosylation-mutated CD97 samples revealed diverse findings, suggesting that the functions of CD97 ECD were complex, and various technologies for function validation should be utilized to avoid single-approach bias when investigating N-glycosylation and auto-proteolysis of CD97. A unique mechanism of concentration-dependent auto-proteolysis of the CD97 EGF1-5 isoform was characterized, suggesting an intermolecular mechanism that is distinct from that of other previously reported CD97 isoforms. The EGF5 and GAIN domains are likely associated with each other as CD97 expression and SAXS data revealed a potential interaction between the two domains. Finally, the GAIN and EGF domains are also important for CD97-HeLa adhesion, whereas N-glycosylation of the CD97 GAIN domain and GPS auto-proteolysis are not required for HeLa cell attachment.
[Mh] Termos MeSH primário: Antígenos CD/metabolismo
Adesão Celular/fisiologia
Proteólise
[Mh] Termos MeSH secundário: Antígenos CD/genética
Glicosilação
Células HeLa
Seres Humanos
Modelos Estruturais
Mutagênese
Peptídeo-N4-(N-acetil-beta-glucosaminil) Asparagina Amidase/metabolismo
Isoformas de Proteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (CD97 protein, human); 0 (Protein Isoforms); EC 3.5.1.52 (Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160920
[St] Status:MEDLINE
[do] DOI:10.1038/aps.2016.89


  8 / 15749 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27765655
[Au] Autor:Rajvanshi S; Choudhary K; Agrawal N
[Ad] Endereço:Department of Zoology, University of Lucknow, Lucknow, 226007, India.
[Ti] Título:Threading: A novel insilico indagation method for genetic characterization of some diplostomoid metacercariae (Digenea:Diplostomidae Poirier, 1886).
[So] Source:Exp Parasitol;171:71-76, 2016 Dec.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The protein encoding zone of Mitochondrial DNA region (inherited from single lineage) seems most suitable and effective for taxonomic, systematic, ecological, evolutionary, DNA barcoding, cryptic species and population studies, exploiting nucleotide/amino acid datasets (1D/2D/3D conformational level). Nowadays, expeditious computerized methods are in trend for analyzing genetic material to demonstrate variations at various levels of protein structures. Structural proteomics have implemented here for genetic identification, differentiation and relationship of species from information rich data of mt COI gene of the family Diplostomidae with inclusion of molecular tools. Various aspects have been utilized herein for re-validation and infallible discrimination of Trematode diplostomoid metacercariae (Tetracotyle lucknowensis Pandey, 1971; T. xenentodoni Chakrabarti, 1970; T. fausti Rai and Pande, 1969; T. muscularius Chakrabarti, 1970 and Diplostomulum minutum Pandey, 1968), the infective stage in the life cycle, causing severe damage to fish host, whose adults are found mainly in fish eating birds and mammals.
[Mh] Termos MeSH primário: Proteínas de Helminto/química
Trematódeos/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Aminoácidos/análise
Animais
Ciclo-Oxigenase 1/genética
DNA Mitocondrial/química
Doenças dos Peixes/parasitologia
Peixes
Proteínas de Helminto/genética
Imagem Tridimensional
Metacercárias/química
Metacercárias/classificação
Metacercárias/genética
Mitocôndrias/enzimologia
Modelos Biológicos
Modelos Estruturais
Conformação Molecular
Filogenia
Conformação Proteica
Proteômica
Trematódeos/química
Trematódeos/classificação
Infecções por Trematódeos/parasitologia
Infecções por Trematódeos/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (DNA, Mitochondrial); 0 (Helminth Proteins); EC 1.14.99.1 (Cyclooxygenase 1)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170112
[Lr] Data última revisão:
170112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161022
[St] Status:MEDLINE


  9 / 15749 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Mineo, Jose R
PubMed Central Texto completo
Texto completo
[PMID]:27313992
[Au] Autor:Bastos LM; Macêdo AG; Silva MV; Santiago FM; Ramos EL; Santos FA; Pirovani CP; Goulart LR; Mineo TW; Mineo JR
[Ad] Endereço:Laboratório de Imunoparasitologia "Dr. Mário Endsfeldz Camargo", Instituto de Ciências Biomédicas, Universidade Federal de UberlândiaUberlândia, Brazil; Laboratório de Nanobiotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de UberlândiaUberlândia, Brazil.
[Ti] Título:Toxoplasma gondii-Derived Synthetic Peptides Containing B- and T-Cell Epitopes from GRA2 Protein Are Able to Enhance Mice Survival in a Model of Experimental Toxoplasmosis.
[So] Source:Front Cell Infect Microbiol;6:59, 2016.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Toxoplasmosis is a zoonosis distributed all over the world, which the etiologic agent is an intracellular protozoan parasite, Toxoplasma gondii. This disease may cause abortions and severe diseases in many warm-blood hosts, including humans, particularly the immunocompromised patients. The parasite specialized secretory organelles, as micronemes, rhoptries and dense granules, are critical for the successful parasitism. The dense granule protein 2 (GRA2) is a parasite immunogenic protein secreted during infections and previous studies have been shown that this parasite component is crucial for the formation of intravacuolar membranous nanotubular network (MNN), as well as for secretion into the vacuole and spatial organization of the parasites within the vacuole. In the present study, we produced a monoclonal antibody to GRA2 (C3C5 mAb, isotype IgG2b), mapped the immunodominant epitope of the protein by phage display and built GRA2 synthetic epitopes to evaluate their ability to protect mice in a model of experimental infection. Our results showed that synthetic peptides for B- and T-cell epitopes are able to improve survival of immunized animals. In contrast with non-immunized animals, the immunized mice with both B- and T-cell epitopes had a better balance of cytokines and demonstrated higher levels of IL-10, IL-4 and IL-17 production, though similar levels of TNF-α and IL-6 were observed. The immunization with both B- and T-cell epitopes resulted in survival rate higher than 85% of the challenged mice. Overall, these results demonstrate that immunization with synthetic epitopes for both B- and T-cells from GRA2 protein can be more effective to protect against infection by T. gondii.
[Mh] Termos MeSH primário: Antígenos de Protozoários/imunologia
Epitopos de Linfócito B/imunologia
Epitopos de Linfócito T/imunologia
Peptídeos/imunologia
Proteínas de Protozoários/imunologia
Vacinas Protozoárias/imunologia
Toxoplasma/imunologia
Toxoplasmose/prevenção & controle
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos
Sequência de Aminoácidos
Animais
Anticorpos Monoclonais/química
Anticorpos Monoclonais/imunologia
Anticorpos Antiprotozoários/sangue
Antígenos de Protozoários/genética
Citocinas/metabolismo
Modelos Animais de Doenças
Feminino
Técnica Indireta de Fluorescência para Anticorpo
Imunidade Humoral
Interleucinas/imunologia
Interleucinas/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Modelos Estruturais
Peptídeos/síntese química
Peptídeos/genética
Conformação Proteica
Vacinas Protozoárias/síntese química
Vacinas Protozoárias/genética
Taxa de Sobrevida
Toxoplasma/química
Toxoplasmose/imunologia
Toxoplasmose/parasitologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Monoclonal); 0 (Antibodies, Protozoan); 0 (Antigens, Protozoan); 0 (Cytokines); 0 (Epitopes, B-Lymphocyte); 0 (Epitopes, T-Lymphocyte); 0 (Gra2 protein, Toxoplasma gondii); 0 (Interleukins); 0 (Peptides); 0 (Protozoan Proteins); 0 (Protozoan Vaccines); 0 (soluble tachyzoite antigen, Toxoplasma gondii)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160618
[St] Status:MEDLINE
[do] DOI:10.3389/fcimb.2016.00059


  10 / 15749 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27283960
[Au] Autor:Wang F; Ye B
[Ad] Endereço:Department of Pathogenic Biology, Chongqing Medical University, Chongqing, 400016, China.
[Ti] Título:In silico cloning and B/T cell epitope prediction of triosephosphate isomerase from Echinococcus granulosus.
[So] Source:Parasitol Res;115(10):3991-8, 2016 Oct.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Cystic echinococcosis is a worldwide zoonosis caused by Echinococcus granulosus. Because the methods of diagnosis and treatment for cystic echinococcosis were limited, it is still necessary to screen target proteins for the development of new anti-hydatidosis vaccine. In this study, the triosephosphate isomerase gene of E. granulosus was in silico cloned. The B cell and T cell epitopes were predicted by bioinformatics methods. The cDNA sequence of EgTIM was composition of 1094 base pairs, with an open reading frame of 753 base pairs. The deduced amino acid sequences were composed of 250 amino acids. Five cross-reactive epitopes, locating on 21aa-35aa, 43aa-57aa, 94aa-107aa, 115-129aa, and 164aa-183aa, could be expected to serve as candidate epitopes in the development of vaccine against E. granulosus. These results could provide bases for gene cloning, recombinant expression, and the designation of anti-hydatidosis vaccine.
[Mh] Termos MeSH primário: Antígenos de Helmintos/imunologia
Equinococose/imunologia
Echinococcus granulosus/enzimologia
Echinococcus granulosus/imunologia
Processamento de Proteína Pós-Traducional
Triose-Fosfato Isomerase/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Antígenos de Helmintos/genética
Antígenos de Helmintos/metabolismo
Clonagem Molecular
Biologia Computacional
DNA Complementar/genética
Equinococose/parasitologia
Echinococcus granulosus/genética
Epitopos de Linfócito B/genética
Epitopos de Linfócito B/imunologia
Epitopos de Linfócito T/genética
Epitopos de Linfócito T/imunologia
Seres Humanos
Modelos Estruturais
Fases de Leitura Aberta/genética
Filogenia
Sinais Direcionadores de Proteínas
Transporte Proteico
Alinhamento de Sequência
Triose-Fosfato Isomerase/genética
Triose-Fosfato Isomerase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Helminth); 0 (DNA, Complementary); 0 (Epitopes, B-Lymphocyte); 0 (Epitopes, T-Lymphocyte); 0 (Protein Sorting Signals); EC 5.3.1.1 (Triose-Phosphate Isomerase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160611
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-016-5166-3



página 1 de 1575 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde