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[PMID]:29178651
[Au] Autor:Laurino MY; Truitt AR; Tenney L; Fisher D; Lindor NM; Veenstra D; Jarvik GP; Newcomb PA; Fullerton SM
[Ad] Endereço:Cancer Prevention Program, Seattle Cancer Care Alliance, Seattle, Washington, USA.
[Ti] Título:Clinical verification of genetic results returned to research participants: findings from a Colon Cancer Family Registry.
[So] Source:Mol Genet Genomic Med;5(6):700-708, 2017 11.
[Is] ISSN:2324-9269
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The extent to which participants act to clinically verify research results is largely unknown. This study examined whether participants who received Lynch syndrome (LS)-related findings pursued researchers' recommendation to clinically verify results with testing performed by a CLIA-certified laboratory. METHODS: The Fred Hutchinson Cancer Research Center site of the multinational Colon Cancer Family Registry offered non-CLIA individual genetic research results to select registry participants (cases and their enrolled relatives) from 2011 to 2013. Participants who elected to receive results were counseled on the importance of verifying results at a CLIA-certified laboratory. Twenty-six (76.5%) of the 34 participants who received genetic results completed 2- and 12-month postdisclosure surveys; 42.3% of these (11/26) participated in a semistructured follow-up interview. RESULTS: Within 12 months of result disclosure, only 4 (15.4%) of 26 participants reported having verified their results in a CLIA-certified laboratory; of these four cases, all research and clinical results were concordant. Reasons for pursuing clinical verification included acting on the recommendation of the research team and informing future clinical care. Those who did not verify results cited lack of insurance coverage and limited perceived personal benefit of clinical verification as reasons for inaction. CONCLUSION: These findings suggest researchers will need to address barriers to seeking clinical verification in order to ensure that the intended benefits of returning genetic research results are realized.
[Mh] Termos MeSH primário: Neoplasias Colorretais Hereditárias sem Polipose/diagnóstico
Testes Genéticos
[Mh] Termos MeSH secundário: Adulto
Idoso
Neoplasias Colorretais Hereditárias sem Polipose/genética
Neoplasias Colorretais Hereditárias sem Polipose/psicologia
Proteínas de Ligação a DNA/genética
Família
Feminino
Pesquisa em Genética
Testes Genéticos/normas
Seres Humanos
Cobertura do Seguro
Laboratórios/normas
Masculino
Meia-Idade
Endonuclease PMS2 de Reparo de Erro de Pareamento/genética
Proteína 1 Homóloga a MutL/genética
Proteína 2 Homóloga a MutS/genética
Sistema de Registros
Inquéritos e Questionários
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (MLH1 protein, human); 0 (Msh6 protein, mouse); EC 3.6.1.- (PMS2 protein, human); EC 3.6.1.3 (MSH2 protein, human); EC 3.6.1.3 (Mismatch Repair Endonuclease PMS2); EC 3.6.1.3 (MutL Protein Homolog 1); EC 3.6.1.3 (MutS Homolog 2 Protein)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1002/mgg3.328


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[PMID]:29340525
[Au] Autor:França CN; Mendes CC; Ferreira CES
[Ad] Endereço:Pós Graduação em Ciências da Saúde, Universidade Santo Amaro, São Paulo, SP, Brasil.
[Ti] Título:Time collection and storage conditions of lipid profile.
[So] Source:Braz J Med Biol Res;51(3):e6955, 2018 Jan 11.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The stability of samples is crucial for getting reliable concentrations of many analytes, including lipid profile. Thus, the goal of this study was to analyze lipid profile under different storage and temperature conditions. This was a prospective study with 809 patients of both genders. Total cholesterol, triglycerides, high-density lipoprotein cholesterol, low density lipoprotein cholesterol and non-high-density lipoprotein were measured within 1 h from collection at room temperature, after 2-3 h of refrigeration (8°C) and after 4-5 h at room temperature. The processing time and storage conditions did not affect the analytes measured. These findings are important for multicenter studies, because of the difficulties related to centrifugation and freezing of samples immediately after collection.
[Mh] Termos MeSH primário: Coleta de Amostras Sanguíneas/métodos
Lipídeos/sangue
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Análise Química do Sangue
Preservação de Sangue
Coleta de Amostras Sanguíneas/instrumentação
Coleta de Amostras Sanguíneas/normas
Colesterol/sangue
Feminino
Seres Humanos
Laboratórios/normas
Lipoproteínas/sangue
Masculino
Meia-Idade
Estudos Prospectivos
Temperatura Ambiente
Fatores de Tempo
Triglicerídeos/sangue
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipids); 0 (Lipoproteins); 0 (Triglycerides); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


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[PMID]:29368465
[Au] Autor:Zwicker K
[Ti] Título:Experimenting with Radium Therapy: In the Laboratory & the Clinic.
[So] Source:Clio Med;95:194-214, 2016.
[Is] ISSN:0045-7183
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Mh] Termos MeSH primário: Centros Médicos Acadêmicos/história
Neoplasias/história
Radioterapia/história
Rádio (Elemento)/história
[Mh] Termos MeSH secundário: História do Século XIX
História do Século XX
Seres Humanos
Laboratórios/história
Neoplasias/radioterapia
Proteção Radiológica/história
Estados Unidos
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Nm] Nome de substância:
W90AYD6R3Q (Radium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM; QIS
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


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[PMID]:29424220
[Ti] Título:The role of extended and whole genome sequencing for tracking transmission of measles and rubella viruses: report from the Global Measles and Rubella Laboratory Network meeting, 2017.
[Ti] Título:Rôle du séquençage génomique étendu et complet pour suivre la transmission des virus rougeoleux et rubéoleux: rapport de la réunion du Réseau mondial de laboratoires de la rougeole et de la rubéole, 2017..
[So] Source:Wkly Epidemiol Rec;93(6):55-9, 2018 Feb 09.
[Is] ISSN:0049-8114
[Cp] País de publicação:Switzerland
[La] Idioma:eng; fre
[Mh] Termos MeSH primário: Genoma Viral
Laboratórios
Vírus do Sarampo/genética
Sarampo/transmissão
Vírus da Rubéola/genética
Rubéola (Sarampo Alemão)/transmissão
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Técnicas de Genotipagem
Seres Humanos
Sarampo/virologia
Rubéola (Sarampo Alemão)/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180210
[St] Status:MEDLINE


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[PMID]:29205831
[Au] Autor:Narasimhan V; Ooi G; Weidlich S; Carson P
[Ad] Endereço:Department of General Surgery, The Alfred Hospital, Melbourne, Victoria, Australia.
[Ti] Título:Response to Re: Laboratory Risk Indicator for Necrotizing Fasciitis score for early diagnosis of necrotizing fasciitis in Darwin.
[So] Source:ANZ J Surg;87(12):1062, 2017 12.
[Is] ISSN:1445-2197
[Cp] País de publicação:Australia
[La] Idioma:eng
[Mh] Termos MeSH primário: Diagnóstico Precoce
Fasciite Necrosante
[Mh] Termos MeSH secundário: Seres Humanos
Laboratórios
Fatores de Risco
[Pt] Tipo de publicação:LETTER; COMMENT
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1111/ans.14228


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[PMID]:29205823
[Au] Autor:Papa N; Sellayah R
[Ad] Endereço:Department of Surgery, Austin Health, The University of Melbourne, Melbourne, Victoria, Australia.
[Ti] Título:Re: Laboratory Risk Indicator for Necrotizing Fasciitis score for early diagnosis of necrotizing fasciitis in Darwin.
[So] Source:ANZ J Surg;87(12):1061, 2017 12.
[Is] ISSN:1445-2197
[Cp] País de publicação:Australia
[La] Idioma:eng
[Mh] Termos MeSH primário: Diagnóstico Precoce
Fasciite Necrosante
[Mh] Termos MeSH secundário: Seres Humanos
Laboratórios
Fatores de Risco
[Pt] Tipo de publicação:LETTER; COMMENT
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1111/ans.14200


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[PMID]:28456552
[Au] Autor:Ziebell K; Chui L; King R; Johnson S; Boerlin P; Johnson RP
[Ad] Endereço:National Microbiology Laboratory at Guelph, PHAC, Guelph, ON, Canada.
[Ti] Título:Subtyping of Canadian isolates of Salmonella Enteritidis using Multiple Locus Variable Number Tandem Repeat Analysis (MLVA) alone and in combination with Pulsed-Field Gel Electrophoresis (PFGE) and phage typing.
[So] Source:J Microbiol Methods;139:29-36, 2017 08.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Salmonella enterica subspecies enterica serovar Enteritidis (SE) is one of the most common causes of human salmonellosis and in Canada currently accounts for over 40% of human cases. Reliable subtyping of isolates is required for outbreak detection and source attribution. However, Pulsed-Field Gel Electrophoresis (PFGE), the current standard subtyping method for Salmonella spp., is compromised by the high genetic homogeneity of SE. Multiple Locus Variable Number Tandem Repeat Analysis (MLVA) was introduced to supplement PFGE, although there is a lack of data on the ability of MLVA to subtype Canadian isolates of SE. Three subtyping methods, PFGE, MLVA and phage typing were compared for their discriminatory power when applied to three panels of Canadian SE isolates: Panel 1: 70 isolates representing the diversity of phage types (PTs) and PFGE subtypes within these PTs; Panel 2: 214 apparently unrelated SE isolates of the most common PTs; and Panel 3: 27 isolates from 10 groups of epidemiologically related strains. For Panel 2 isolates, four MLVA subtypes were shared among 74% of unrelated isolates and in Panel 3 isolates, one MLVA subtype accounted for 62% of the isolates. For all panels, combining results from PFGE, MLVA and PT gave the best discrimination, except in Panel 1, where the combination of PT and PFGE was equally as high, due to the selection criteria for this panel. However, none of these methods is sufficiently discriminatory alone for reliable outbreak detection or source attribution, and must be applied together to achieve sufficient discrimination for practical purposes. Even then, some large clusters were not differentiated adequately. More discriminatory methods are required for reliable subtyping of this genetically highly homogeneous serovar. This need will likely be met by whole genome sequence analysis given the recent promising reports and as more laboratories implement this tool for outbreak response and surveillance.
[Mh] Termos MeSH primário: Técnicas de Tipagem Bacteriana/métodos
Tipagem de Bacteriófagos
Eletroforese em Gel de Campo Pulsado
Repetições Minissatélites
Salmonella enteritidis/classificação
Salmonella enteritidis/genética
[Mh] Termos MeSH secundário: Animais
Canadá
DNA Bacteriano/genética
Surtos de Doenças
Seres Humanos
Laboratórios
Infecções por Salmonella/microbiologia
Salmonella enteritidis/isolamento & purificação
Sorogrupo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


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[PMID]:28464797
[Au] Autor:Tesfaye A; Fiseha D; Assefa D; Klinkenberg E; Balanco S; Langley I
[Ad] Endereço:Addis Ababa City Government Health Bureau, Addis Ababa, Ethiopia. atabrish@gmail.com.
[Ti] Título:Modeling the patient and health system impacts of alternative xpert® MTB/RIF algorithms for the diagnosis of pulmonary tuberculosis in Addis Ababa, Ethiopia.
[So] Source:BMC Infect Dis;17(1):318, 2017 05 02.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: To reduce global tuberculosis (TB) burden, the active disease must be diagnosed quickly and accurately and patients should be treated and cured. In Ethiopia, TB diagnosis mainly relies on spot-morning-spot (SMS) sputum sample smear analysis using Ziehl-Neelsen staining techniques (ZN). Since 2014 targeted use of xpert has been implemented. New diagnostic techniques have higher sensitivity and are likely to detect more cases if routinely implemented. The objective of our study was to project the effects of alternative diagnostic algorithms on the patient, health system, and costs, and identify cost-effective algorithms that increase TB case detection in Addis Ababa, Ethiopia. METHODS: An observational quantitative modeling framework was applied using the Virtual Implementation approach. The model was designed to represent the operational and epidemiological context of Addis Ababa, the capital city of Ethiopia. We compared eight diagnostic algorithm with ZN microscopy, light emitting diode (LED) fluorescence microscopy and Xpert MTB/RIF. Interventions with an annualized cost per averted disability adjusted life year (DALY) of less than the Gross Domestic Product (GDP) per capita are considered cost-effective interventions. RESULTS: With a cost lower than the average per-capita GDP (US$690 for Ethiopia) for each averted disability adjusted life year (DALY), three of the modeled algorithms are cost-effective. Implementing them would have important patient, health system, and population-level effects in the context of Addis Ababa ❖ The full roll-out of Xpert MTB/RIF as the primary test for all presumptive TB cases would avert 91170 DALYs (95% credible interval [CrI] 54888 - 127448) with an additional health system cost of US$ 11.6 million over the next 10 years. The incremental cost-effectiveness ratio (ICER) is $370 per DALY averted. ❖ Same day LED fluorescence microscopy for all presumptive TB cases combined with Xpert MTB/RIF targeted to HIV-positive and High multidrug resistant (MDR) risk groups would avert 73600 DALYs( 95% CrI 48373 - 99214) with an additional cost of US$5.1 million over the next 10 years. The ICER is $169per DALY averted. ❖ Same-day LED fluorescence microscopy for all presumptive TB cases (and no Xpert MTB/RIF) would avert 43580 DALYs with a reduction cost of US$ 0.2 million over the next 10years. The ICER is $13 per DALY averted. CONCLUSIONS: The full roll-out of Xpert MTB/RIF is predicted to be the best option to substantially reduce the TB burden in Addis Ababa and is considered cost effective. However, the investment cost to implement this is far beyond the budget of the national TB control program. Targeted use of Xpert MTB/RIF for HIV positive and high MDR risk groups with same-day LED fluorescence microscopy for all other presumptive TB cases is an affordable alternative.
[Mh] Termos MeSH primário: Algoritmos
Diagnóstico por Computador/métodos
Tuberculose Pulmonar/diagnóstico
[Mh] Termos MeSH secundário: Análise Custo-Benefício
Assistência à Saúde/economia
Diagnóstico por Computador/economia
Etiópia
Feminino
Infecções por HIV/microbiologia
Seres Humanos
Laboratórios/economia
Masculino
Microscopia de Fluorescência/economia
Microscopia de Fluorescência/métodos
Mycobacterium tuberculosis/efeitos dos fármacos
Anos de Vida Ajustados por Qualidade de Vida
Sensibilidade e Especificidade
Escarro/microbiologia
Tuberculose Resistente a Múltiplos Medicamentos/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180120
[Lr] Data última revisão:
180120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2417-6


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[PMID]:29190268
[Au] Autor:Tapp L; Ramsey JG; Wen A; Gerona R
[Ti] Título:Synthetic Cannabinoid and Mitragynine Exposure of Law Enforcement Agents During the Raid of an Illegal Laboratory - Nevada, 2014.
[So] Source:MMWR Morb Mortal Wkly Rep;66(47):1291-1294, 2017 Dec 01.
[Is] ISSN:1545-861X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Synthetic cannabinoids (SCs), commonly known by the street name "Spice," are designer drugs of abuse that mimic the psychoactive effects of marijuana. Intentional SC use has resulted in multiple toxicities (1,2), but little is known about occupational SC exposure. After a federal agency's law enforcement personnel in Nevada reported irritability and feeling "high" after raiding illegal SC laboratories and processing seized SCs, a request for a health hazard evaluation was made by the agency to CDC's National Institute for Occupational Safety and Health (NIOSH) in 2014 to evaluate agents' occupational SC exposures. After making the request for a health hazard evaluation, federal agents conducted a raid of an illegal SC laboratory, with assistance from local law enforcement and Drug Enforcement Administration (DEA) personnel and with NIOSH investigators observing from a distance. After the raid, agents collected and processed material evidence. NIOSH investigators tested agents' urine for SC levels before and after the raid and measured SCs in the air and on surfaces after the raid. DEA determined that AB-PINACA (an SC compound) and mitragynine (a plant material with opium-like effects, also known as "kratom") were present in the illegal laboratory. AB-PINACA, its metabolites, and mitragynine were not detected in agents' urine before the raid; however, one or more of these substances was found in the urine of six of nine agents after the raid and processing of the SC evidence. AB-PINACA was detected in one surface wipe sample from the SC laboratory; none was detected in the air in the laboratory or in the offices of the law enforcement agency where the materials were processed after the raid. No policies were in place regarding work practices and use of personal protective equipment (PPE) during raids and evidence processing. To protect agents from SC exposures, NIOSH recommended that the agency require agents to wear a minimum level of PPE (e.g., protective gloves and disposable clothing) and undergo training in PPE and in handling and storing of contaminated evidence from SC laboratory raids. Showers and locker rooms also need to be provided so that agents can reduce contamination and prevent take-home exposure.
[Mh] Termos MeSH primário: Canabinoides/urina
Drogas Desenhadas
Controle de Medicamentos e Entorpecentes/legislação & jurisprudência
Laboratórios/legislação & jurisprudência
Aplicação da Lei
Exposição Ocupacional/análise
Alcaloides de Triptamina e Secologanina/urina
[Mh] Termos MeSH secundário: Adulto
Seres Humanos
Indazóis/urina
Masculino
Meia-Idade
Nevada
Equipamento de Proteção Individual/utilização
Valina/análogos & derivados
Valina/urina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cannabinoids); 0 (Designer Drugs); 0 (Indazoles); 0 (N-(1-amino-3-methyl-1-oxobutan-2-yl)-1-pentyl-1H-indazole-3-carboxamide); 0 (Secologanin Tryptamine Alkaloids); EP479K822J (mitragynine); HG18B9YRS7 (Valine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180119
[Lr] Data última revisão:
180119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.15585/mmwr.mm6647a3


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[PMID]:29261759
[Au] Autor:Chung HJ; Song YK; Hong SK; Hwang SH; Seo HS; Whang DH; Nam MH; Lee DH
[Ad] Endereço:Department of Laboratory Medicine, National Cancer Center, Goyang, South Korea.
[Ti] Título:Implementation of biological variation-based analytical performance specifications in the laboratory: Stringent evaluation of Improvacutor blood collection tubes.
[So] Source:PLoS One;12(12):e0189882, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recently, because the quality of laboratory analyses has increased along with the need for quality improvement, several external quality control bodies have adapted performance specifications using the Desirable Biological Variation Database, termed "Ricos goals"; these criteria are more stringent than those presented in CLIA 88. In this study, we aimed to validate newly introduced serum separator tubes, Improvacutor, for routine clinical chemistry testing in accordance with Ricos goals and CLIA 88. Blood samples were collected from 100 volunteers into three types of serum vacuum tubes: Greiner Vacuette, Becton Dickinson (BD) Vacutainer, and Improve Improvacutor. The samples were subjected to 16 routine chemistry tests using a TBA-200fr NEO chemistry autoanalyzer. In the comparison analysis, all 16 test results were acceptable according to CLIA 88. However, in the comparison of Improve and BD tubes, creatinine showed 4.31% (+0.08 µmol/L) bias. This slightly exceeded the Desirable Specification for Inaccuracy Ricos limit of ±3.96%, but still satisfied the CLIS88 limit of ±26.52 µmol/L. The remaining 15 analytes performed acceptably according to the Desirable Specifications of Ricos. The correlation coefficient of 12 analytes was greater than 0.95 in Passing-Bablok regression analysis among the three tubes, but was lower for four analytes: calcium, sodium, potassium, and chloride. In the stability assay, only potassium tested in the Greiner tube revealed a larger positive bias (2.18%) than the Ricos Desirable Specification for Inaccuracy based on biologic variation (1.8%). The BD tube also showed a positive bias of 1.74%, whereas the new Improve tube showed the smallest positive bias of 1.17% in potassium level after 72 h storage. Thus, the results of this study demonstrate that recently introduced analytical performance specifications based on components of biological variation (Rico's goal) could be extended to criterion for performance evaluation and applied.
[Mh] Termos MeSH primário: Coleta de Amostras Sanguíneas/instrumentação
Laboratórios
[Mh] Termos MeSH secundário: Adulto
Idoso
Nitrogênio da Ureia Sanguínea
Cálcio/sangue
Creatinina/sangue
Feminino
Seres Humanos
Masculino
Meia-Idade
Temperatura Ambiente
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
AYI8EX34EU (Creatinine); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189882



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