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Pesquisa : L01.178.682.192.836.147 [Categoria DeCS]
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[PMID]:29371626
[Au] Autor:Carradec Q; Pelletier E; Da Silva C; Alberti A; Seeleuthner Y; Blanc-Mathieu R; Lima-Mendez G; Rocha F; Tirichine L; Labadie K; Kirilovsky A; Bertrand A; Engelen S; Madoui MA; Méheust R; Poulain J; Romac S; Richter DJ; Yoshikawa G; Dimier C; Kandels-Lewis S; Picheral M; Searson S; Jaillon O; Aury JM; Karsenti E; Sullivan MB; Sunagawa S; Bork P; Not F; Hingamp P; Raes J; Guidi L; Ogata H; de Vargas C; Iudicone D; Bowler C; Wincker P; Tara Oceans Coordinators
[Ad] Endereço:CEA - Institut de Biologie François Jacob, Genoscope, Evry, 91057, France.
[Ti] Título:A global ocean atlas of eukaryotic genes.
[So] Source:Nat Commun;9(1):373, 2018 01 25.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:While our knowledge about the roles of microbes and viruses in the ocean has increased tremendously due to recent advances in genomics and metagenomics, research on marine microbial eukaryotes and zooplankton has benefited much less from these new technologies because of their larger genomes, their enormous diversity, and largely unexplored physiologies. Here, we use a metatranscriptomics approach to capture expressed genes in open ocean Tara Oceans stations across four organismal size fractions. The individual sequence reads cluster into 116 million unigenes representing the largest reference collection of eukaryotic transcripts from any single biome. The catalog is used to unveil functions expressed by eukaryotic marine plankton, and to assess their functional biogeography. Almost half of the sequences have no similarity with known proteins, and a great number belong to new gene families with a restricted distribution in the ocean. Overall, the resource provides the foundations for exploring the roles of marine eukaryotes in ocean ecology and biogeochemistry.
[Mh] Termos MeSH primário: Organismos Aquáticos
Eucariotos/genética
Células Eucarióticas/metabolismo
Metagenoma
Filogenia
Zooplâncton/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Atlas como Assunto
Bactérias/classificação
Bactérias/genética
Biodiversidade
Ecossistema
Eucariotos/classificação
Células Eucarióticas/citologia
Metagenômica/métodos
Oceanos e Mares
Fitoplâncton/classificação
Fitoplâncton/genética
Água do Mar
Vírus/classificação
Vírus/genética
Zooplâncton/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02342-1


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[PMID]:29096072
[Au] Autor:Ecker JR; Geschwind DH; Kriegstein AR; Ngai J; Osten P; Polioudakis D; Regev A; Sestan N; Wickersham IR; Zeng H
[Ad] Endereço:Genomic Analysis Laboratory and Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
[Ti] Título:The BRAIN Initiative Cell Census Consortium: Lessons Learned toward Generating a Comprehensive Brain Cell Atlas.
[So] Source:Neuron;96(3):542-557, 2017 Nov 01.
[Is] ISSN:1097-4199
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A comprehensive characterization of neuronal cell types, their distributions, and patterns of connectivity is critical for understanding the properties of neural circuits and how they generate behaviors. Here we review the experiences of the BRAIN Initiative Cell Census Consortium, ten pilot projects funded by the U.S. BRAIN Initiative, in developing, validating, and scaling up emerging genomic and anatomical mapping technologies for creating a complete inventory of neuronal cell types and their connections in multiple species and during development. These projects lay the foundation for a larger and longer-term effort to generate whole-brain cell atlases in species including mice and humans.
[Mh] Termos MeSH primário: Atlas como Assunto
Mapeamento Encefálico/métodos
Encéfalo/citologia
Encéfalo/fisiologia
Rede Nervosa/citologia
Rede Nervosa/fisiologia
[Mh] Termos MeSH secundário: Animais
Encéfalo/anatomia & histologia
Mapeamento Encefálico/tendências
Seres Humanos
Rede Nervosa/anatomia & histologia
Projetos Piloto
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171103
[St] Status:MEDLINE


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[PMID]:28768315
[Au] Autor:Abbasi J
[Ti] Título:An International Human Cell Atlas Consortium Takes Shape.
[So] Source:JAMA;318(8):685-686, 2017 Aug 22.
[Is] ISSN:1538-3598
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Atlas como Assunto
Células/citologia
[Mh] Termos MeSH secundário: Anatomia Artística
Animais
Células/ultraestrutura
Seres Humanos
Internacionalidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE
[do] DOI:10.1001/jama.2017.5640


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[PMID]:28682347
[Au] Autor:Nowogrodzki A
[Ti] Título:How to build a human cell atlas.
[So] Source:Nature;547(7661):24-26, 2017 07 05.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Atlas como Assunto
Rastreamento de Células
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1038/547024a


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[PMID]:28489821
[Au] Autor:Hildebrand DGC; Cicconet M; Torres RM; Choi W; Quan TM; Moon J; Wetzel AW; Scott Champion A; Graham BJ; Randlett O; Plummer GS; Portugues R; Bianco IH; Saalfeld S; Baden AD; Lillaney K; Burns R; Vogelstein JT; Schier AF; Lee WA; Jeong WK; Lichtman JW; Engert F
[Ad] Endereço:Graduate Program in Neuroscience, Division of Medical Sciences, Graduate School of Arts and Sciences, Harvard University, Cambridge, Massachusetts, USA.
[Ti] Título:Whole-brain serial-section electron microscopy in larval zebrafish.
[So] Source:Nature;545(7654):345-349, 2017 05 18.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:High-resolution serial-section electron microscopy (ssEM) makes it possible to investigate the dense meshwork of axons, dendrites, and synapses that form neuronal circuits. However, the imaging scale required to comprehensively reconstruct these structures is more than ten orders of magnitude smaller than the spatial extents occupied by networks of interconnected neurons, some of which span nearly the entire brain. Difficulties in generating and handling data for large volumes at nanoscale resolution have thus restricted vertebrate studies to fragments of circuits. These efforts were recently transformed by advances in computing, sample handling, and imaging techniques, but high-resolution examination of entire brains remains a challenge. Here, we present ssEM data for the complete brain of a larval zebrafish (Danio rerio) at 5.5 days post-fertilization. Our approach utilizes multiple rounds of targeted imaging at different scales to reduce acquisition time and data management requirements. The resulting dataset can be analysed to reconstruct neuronal processes, permitting us to survey all myelinated axons (the projectome). These reconstructions enable precise investigations of neuronal morphology, which reveal remarkable bilateral symmetry in myelinated reticulospinal and lateral line afferent axons. We further set the stage for whole-brain structure-function comparisons by co-registering functional reference atlases and in vivo two-photon fluorescence microscopy data from the same specimen. All obtained images and reconstructions are provided as an open-access resource.
[Mh] Termos MeSH primário: Encéfalo/ultraestrutura
Microscopia Eletrônica
Peixe-Zebra
[Mh] Termos MeSH secundário: Anatomia Artística
Animais
Atlas como Assunto
Axônios/metabolismo
Axônios/ultraestrutura
Encéfalo/anatomia & histologia
Encéfalo/citologia
Conjuntos de Dados como Assunto
Larva/anatomia & histologia
Larva/citologia
Larva/ultraestrutura
Microscopia de Fluorescência por Excitação Multifotônica
Publicação de Acesso Aberto
Peixe-Zebra/anatomia & histologia
Peixe-Zebra/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170511
[St] Status:MEDLINE
[do] DOI:10.1038/nature22356


  6 / 848 MEDLINE  
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[PMID]:28320311
[Au] Autor:Janusonis S
[Ad] Endereço:Department of Psychological and Brain Sciences, University of California, Santa Barbara, CA, 93106-9660, USA. skirmantas.janusonis@psych.ucsb.edu.
[Ti] Título:A receptor-based analysis of local ecosystems in the human brain.
[So] Source:BMC Neurosci;18(1):33, 2017 Mar 20.
[Is] ISSN:1471-2202
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: As a complex system, the brain is a self-organizing entity that depends on local interactions among cells. Its regions (anatomically defined nuclei and areas) can be conceptualized as cellular ecosystems, but the similarity of their functional profiles is poorly understood. The study used the Allen Human Brain Atlas to classify 169 brain regions into hierarchically-organized environments based on their expression of 100 G protein-coupled neurotransmitter receptors, with no a priori reference to the regions' positions in the brain's anatomy or function. The analysis was based on hierarchical clustering, and multiscale bootstrap resampling was used to estimate the reliability of detected clusters. RESULTS: The study presents the first unbiased, hierarchical tree of functional environments in the human brain. The similarity of brain regions was strongly influenced by their anatomical proximity, even when they belonged to different functional systems. Generally, spatial vicinity trumped long-range projections or network connectivity. The main cluster of brain regions excluded the dentate gyrus of the hippocampus. The nuclei of the amygdala formed a cluster irrespective of their striatal or pallial origin. In its receptor profile, the hypothalamus was more closely associated with the midbrain than with the thalamus. The cerebellar cortical areas formed a tight and exclusive cluster. Most of the neocortical areas (with the exception of some occipital areas) clustered in a large, statistically well supported group that included no other brain regions. CONCLUSIONS: This study adds a new dimension to the established classifications of brain divisions. In a single framework, they are reconsidered at multiple scales-from individual nuclei and areas to their groups to the entire brain. The analysis provides support for predictive models of brain self-organization and adaptation.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Receptores Acoplados a Proteínas-G/metabolismo
Receptores de Neurotransmissores/metabolismo
[Mh] Termos MeSH secundário: Adulto
Atlas como Assunto
Encéfalo/anatomia & histologia
Análise por Conglomerados
Feminino
Seres Humanos
Masculino
Meia-Idade
RNA Mensageiro/metabolismo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (Receptors, G-Protein-Coupled); 0 (Receptors, Neurotransmitter)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1186/s12868-017-0355-2


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[PMID]:28298886
[Au] Autor:Dempsey B; Le S; Turner A; Bokiniec P; Ramadas R; Bjaalie JG; Menuet C; Neve R; Allen AM; Goodchild AK; McMullan S
[Ad] Endereço:Faculty of Medicine and Health Sciences, Neurobiology of Vital Systems, Macquarie University Sydney, NSW, Australia.
[Ti] Título:Mapping and Analysis of the Connectome of Sympathetic Premotor Neurons in the Rostral Ventrolateral Medulla of the Rat Using a Volumetric Brain Atlas.
[So] Source:Front Neural Circuits;11:9, 2017.
[Is] ISSN:1662-5110
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Spinally projecting neurons in the rostral ventrolateral medulla (RVLM) play a critical role in the generation of vasomotor sympathetic tone and are thought to receive convergent input from neurons at every level of the neuraxis; the factors that determine their ongoing activity remain unresolved. In this study we use a genetically restricted viral tracing strategy to definitively map their spatially diffuse connectome. We infected bulbospinal RVLM neurons with a recombinant rabies variant that drives reporter expression in monosynaptically connected input neurons and mapped their distribution using an MRI-based volumetric atlas and a novel image alignment and visualization tool that efficiently translates the positions of neurons captured in conventional photomicrographs to Cartesian coordinates. We identified prominent inputs from well-established neurohumoral and viscero-sympathetic sensory actuators, medullary autonomic and respiratory subnuclei, and supramedullary autonomic nuclei. The majority of inputs lay within the brainstem (88-94%), and included putative respiratory neurons in the pre-Bötzinger Complex and post-inspiratory complex that are therefore likely to underlie respiratory-sympathetic coupling. We also discovered a substantial and previously unrecognized input from the region immediately ventral to nucleus prepositus hypoglossi. In contrast, RVLM sympathetic premotor neurons were only sparsely innervated by suprapontine structures including the paraventricular nucleus, lateral hypothalamus, periaqueductal gray, and superior colliculus, and we found almost no evidence of direct inputs from the cortex or amygdala. Our approach can be used to quantify, standardize and share complete neuroanatomical datasets, and therefore provides researchers with a platform for presentation, analysis and independent reanalysis of connectomic data.
[Mh] Termos MeSH primário: Encéfalo/anatomia & histologia
Conectoma/métodos
Neurônios/fisiologia
Medula Espinal/anatomia & histologia
[Mh] Termos MeSH secundário: Animais
Atlas como Assunto
Vetores Genéticos
Imagem por Ressonância Magnética
Masculino
Bulbo/anatomia & histologia
Vírus da Raiva
Ratos
Ratos Sprague-Dawley
Simplexvirus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.3389/fncir.2017.00009


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[PMID]:28288465
[Au] Autor:Serup J
[Ad] Endereço:The 'Tattoo Clinic', Department of Dermatology, Bispebjerg University Hospital, Copenhagen, Denmark.
[Ti] Título:Atlas of Illustrative Cases of Tattoo Complications.
[So] Source:Curr Probl Dermatol;52:139-229, 2017.
[Is] ISSN:1662-2944
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Tattoos, and tattoo complications as well, are colorful and visually flashy. A clinical outlook provides important clues to diagnosis by pattern recognition. This atlas,which is a report of 79 case illustrations, is made as a practical tool and vade mecum for the clinician. The atlas can be used in the office and as a bedside tool. It includes characteristic photos, and each case is accompanied with a brief medical history, a comment with learning points, the final diagnosis, and a review of therapeutic options. This spectrum of cases illustrates the different diagnostic entities according to the newly proposed Bispebjerg University Hospital ('Tattoo Clinic') diagnostic system of tattoo complications, a system reported to the 11th revision of the WHO disease classification system. Cases include allergies predominantly seen in red tattoos manifesting the characteristic patterns of plaque elevation, excessive hyperkeratosis, ulcerating reactions, urticaria, and generalized skin rash. Each type of reaction has typical clues to diagnosis and a typical disease course. 'Cross-sensitivity' allergic reactions in old tattoos of the same color as the trigger tattoo are also illustrated. Reactions in black tattoos are dominated by a pattern with papulonodular reactions, which are nonallergic anddue to pigment agglomeration in the skin. Reactions may be granulomatous and a marker of systemic sarcoidosis, sometimes associated with a general outbreak in the skin in black tattoos, e.g. 'rush phenomenon'. Bacterial infections are also illustrated. The technical hazards of tattooing and sequels as well as unwanted effects of tattoo removal by lasers are shown along with severe scarring following tattoo removal by caustics. This atlas is a tutorial in modern medical and surgical treatments of tattoo complications.
[Mh] Termos MeSH primário: Dermatopatias/diagnóstico
Dermatopatias/etiologia
Tatuagem/efeitos adversos
[Mh] Termos MeSH secundário: Atlas como Assunto
Cor
Dinamarca
Feminino
Granuloma de Corpo Estranho/diagnóstico
Granuloma de Corpo Estranho/etiologia
Seres Humanos
Hipersensibilidade/diagnóstico
Hipersensibilidade/etiologia
Tinta
Sarcoidose/diagnóstico
Sarcoidose/etiologia
Dermatopatias/terapia
Dermatopatias Bacterianas/diagnóstico
Dermatopatias Bacterianas/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE
[do] DOI:10.1159/000453379


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[PMID]:28251150
[Au] Autor:Zheng X; Wei W; Huang Q; Song S; Wan J; Huang G
[Ad] Endereço:Department of Automation, The School of Electrical Engineering Information, Sichuan University, Chengdu, China; Department of Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
[Ti] Título:A Computer-Aided Analysis Method of SPECT Brain Images for Quantitative Treatment Monitoring: Performance Evaluations and Clinical Applications.
[So] Source:Biomed Res Int;2017:1962181, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective and quantitative analysis of longitudinal single photon emission computed tomography (SPECT) images are significant for the treatment monitoring of brain disorders. Therefore, a computer aided analysis (CAA) method is introduced to extract a change-rate map (CRM) as a parametric image for quantifying the changes of regional cerebral blood flow (rCBF) in longitudinal SPECT brain images. The performances of the CAA-CRM approach in treatment monitoring are evaluated by the computer simulations and clinical applications. The results of computer simulations show that the derived CRMs have high similarities with their ground truths when the lesion size is larger than system spatial resolution and the change rate is higher than 20%. In clinical applications, the CAA-CRM approach is used to assess the treatment of 50 patients with brain ischemia. The results demonstrate that CAA-CRM approach has a 93.4% accuracy of recovered region's localization. Moreover, the quantitative indexes of recovered regions derived from CRM are all significantly different among the groups and highly correlated with the experienced clinical diagnosis. In conclusion, the proposed CAA-CRM approach provides a convenient solution to generate a parametric image and derive the quantitative indexes from the longitudinal SPECT brain images for treatment monitoring.
[Mh] Termos MeSH primário: Encéfalo/diagnóstico por imagem
Monitoramento de Medicamentos
Processamento de Imagem Assistida por Computador/métodos
Tomografia Computadorizada de Emissão de Fóton Único/métodos
[Mh] Termos MeSH secundário: Anatomia Artística
Atlas como Assunto
Simulação por Computador
Feminino
Seres Humanos
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1155/2017/1962181


  10 / 848 MEDLINE  
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[PMID]:28242137
[Au] Autor:Guerreiro F; Burgos N; Dunlop A; Wong K; Petkar I; Nutting C; Harrington K; Bhide S; Newbold K; Dearnaley D; deSouza NM; Morgan VA; McClelland J; Nill S; Cardoso MJ; Ourselin S; Oelfke U; Knopf AC
[Ad] Endereço:Faculty of Sciences, University of Lisbon, Campo Grande, Portugal; Division of Radiotherapy and Imaging, The Institute of Cancer Research, London, United Kingdom. Electronic address: filipaguerreiro23@gmail.com.
[Ti] Título:Evaluation of a multi-atlas CT synthesis approach for MRI-only radiotherapy treatment planning.
[So] Source:Phys Med;35:7-17, 2017 Mar.
[Is] ISSN:1724-191X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND PURPOSE: Computed tomography (CT) imaging is the current gold standard for radiotherapy treatment planning (RTP). The establishment of a magnetic resonance imaging (MRI) only RTP workflow requires the generation of a synthetic CT (sCT) for dose calculation. This study evaluates the feasibility of using a multi-atlas sCT synthesis approach (sCT ) for head and neck and prostate patients. MATERIAL AND METHODS: The multi-atlas method was based on pairs of non-rigidly aligned MR and CT images. The sCT was obtained by registering the MRI atlases to the patient's MRI and by fusing the mapped atlases according to morphological similarity to the patient. For comparison, a bulk density assignment approach (sCT ) was also evaluated. The sCT was obtained by assigning density values to MRI tissue classes (air, bone and soft-tissue). After evaluating the synthesis accuracy of the sCTs (mean absolute error), sCT-based delineations were geometrically compared to the CT-based delineations. Clinical plans were re-calculated on both sCTs and a dose-volume histogram and a gamma analysis was performed using the CT dose as ground truth. RESULTS: Results showed that both sCTs were suitable to perform clinical dose calculations with mean dose differences less than 1% for both the planning target volume and the organs at risk. However, only the sCT provided an accurate and automatic delineation of bone. CONCLUSIONS: Combining MR delineations with our multi-atlas CT synthesis method could enable MRI-only treatment planning and thus improve the dosimetric and geometric accuracy of the treatment, and reduce the number of imaging procedures.
[Mh] Termos MeSH primário: Atlas como Assunto
Imagem por Ressonância Magnética/métodos
Planejamento da Radioterapia Assistida por Computador/métodos
Tomografia Computadorizada por Raios X/métodos
[Mh] Termos MeSH secundário: Algoritmos
Estudos de Viabilidade
Seres Humanos
Masculino
Neoplasias Orofaríngeas/diagnóstico por imagem
Neoplasias Orofaríngeas/radioterapia
Neoplasias da Próstata/diagnóstico por imagem
Neoplasias da Próstata/radioterapia
Dosagem Radioterapêutica
Radioterapia de Intensidade Modulada/métodos
Estudos Retrospectivos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE



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