Base de dados : MEDLINE
Pesquisa : L01.453.245.667 [Categoria DeCS]
Referências encontradas : 647880 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 64788 ir para página                         

  1 / 647880 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28743231
[Au] Autor:Xu X; Li G; Li L; Su Z; Chen C
[Ad] Endereço:College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
[Ti] Título:Genome-wide comparative analysis of putative Pth11-related G protein-coupled receptors in fungi belonging to Pezizomycotina.
[So] Source:BMC Microbiol;17(1):166, 2017 Jul 25.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: G-protein coupled receptors (GPCRs) are the largest family of transmembrane receptors in fungi, where they play important roles in signal transduction. Among them, the Pth11-related GPCRs form a large and divergent protein family, and are only found in fungi in Pezizomycotina. However, the evolutionary process and potential functions of Pth11-related GPCRs remain largely unknown. RESULTS: Twenty genomes of fungi in Pezizomycotina covering different nutritional strategies were mined for putative Pth11-related GPCRs. Phytopathogens encode much more putative Pth11-related GPCRs than symbionts, saprophytes, or entomopathogens. Based on the phylogenetic tree, these GPCRs can be divided into nine clades, with each clade containing fungi in different taxonomic orders. Instead of fungi from the same order, those fungi with similar nutritional strategies were inclined to share orthologs of putative Pth11-related GPCRs. Most of the CFEM domain-containing Pth11-related GPCRs, which were only included in two clades, were detected in phytopathogens. Furthermore, many putative Pth11-related GPCR genes of phytopathogens were upregulated during invasive plant infection, but downregulated under biotic stress. The expressions of putative Pth11-related GPCR genes of saprophytes and entomopathogens could be affected by nutrient conditions, especially the carbon source. The gene expressions revealed that Pth11-related GPCRs could respond to biotic/abiotic stress and invasive plant infection with different expression patterns. CONCLUSION: Our results indicated that the Pth11-related GPCRs existed before the diversification of Pezizomycotina and have been gained and/or lost several times during the evolutionary process. Tandem duplications and trophic variations have been important factors in this evolution.
[Mh] Termos MeSH primário: Ascomicetos/genética
Proteínas Fúngicas/química
Genoma Fúngico
Receptores Acoplados a Proteínas-G/química
Receptores Acoplados a Proteínas-G/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Ascomicetos/química
Ascomicetos/classificação
Ascomicetos/metabolismo
Evolução Molecular
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Dados de Sequência Molecular
Filogenia
Receptores Acoplados a Proteínas-G/genética
Alinhamento de Sequência
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Receptors, G-Protein-Coupled)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-017-1076-5


  2 / 647880 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29191658
[Au] Autor:Sang M; Wu Q; Xi X; Ma C; Wang L; Zhou M; Burrows JF; Chen T
[Ad] Endereço:School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, China; School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK.
[Ti] Título:Identification and target-modifications of temporin-PE: A novel antimicrobial peptide in the defensive skin secretions of the edible frog, Pelophylax kl. esculentus.
[So] Source:Biochem Biophys Res Commun;495(4):2539-2546, 2018 01 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A potent natural antimicrobial peptide named temporin-PE was identified and encoded from the skin secretions of Pelophylax kl. esculentus via "shotgun" cloning and LC-MS/MS fragmentation analysis. Target-modifications were carried out to further enhance the antimicrobial and anti-proliferative bioactivities, whilst decreasing the hemolytic effect. A range of bioassays demonstrated that replacing a proline with a tyrosine residue resulted in a loss of the bioactivity against Gram-negative bacteria, but dramatically improved the hemolytic and anti-proliferative activity, indicating the FLP- motif influences the hemolytic activity of temporins. Moreover, the coupling of TAT to the peptide dramatically improved its antimicrobial activity, indicating coupling TAT to these peptides could be considered as a potential tool to improve their antimicrobial activity. Overall, we have shown that targeted modifications of this natural antimicrobial peptide can adjust its bioactivities to help its development as an antibiotic or anti-proliferative agent.
[Mh] Termos MeSH primário: Proteínas de Anfíbios/química
Peptídeos Catiônicos Antimicrobianos/administração & dosagem
Peptídeos Catiônicos Antimicrobianos/química
Fenômenos Fisiológicos Bacterianos/efeitos dos fármacos
Rana esculenta/metabolismo
Pele/secreção
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Anfíbios/administração & dosagem
Proteínas de Anfíbios/secreção
Animais
Antibacterianos/administração & dosagem
Antibacterianos/química
Antibacterianos/isolamento & purificação
Peptídeos Catiônicos Antimicrobianos/secreção
Sobrevivência Celular/efeitos dos fármacos
Dados de Sequência Molecular
Pele/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amphibian Proteins); 0 (Anti-Bacterial Agents); 0 (Antimicrobial Cationic Peptides); 0 (temporin-PE, Pelophylax kl. esculentus)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE


  3 / 647880 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
[PMID]:29236923
[Au] Autor:Jorge S; Kremer FS; Oliveira NR; Navarro GOSV; Guimarães AM; Sanchez CD; Woloski RDDS; Ridieri KF; Campos VF; Pinto LDS; Dellagostin OA
[Ad] Endereço:Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
[Ti] Título:Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain.
[So] Source:Mem Inst Oswaldo Cruz;113(2):80-86, 2018 Feb.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:BACKGROUND Leptospirosis is the most widespread zoonotic disease. It is caused by infection with pathogenic Leptospira species, of which over 300 serovars have been described. The accurate identification of the causative Leptospira spp. is required to ascertain the pathogenic status of the local isolates. OBJECTIVES This study aimed to obtain the complete genome sequence of a virulent Leptospira interrogans strain isolated from southern Brazil and to describe its genetic features. METHODS The whole genome was sequenced by next-generation sequencing (Ion Torrent). The genome was assembled, scaffolded, annotated, and manually reviewed. Mutations were identified based on a variant calling analysis using the genome of L. interrogans strain Fiocruz L1-130 as a reference. FINDINGS The entire genome had an average GC content of 35%. The variant calling analysis identified 119 single nucleotide polymorphisms (SNPs), from which 30 led to a missense mutation. The structural analyses identified potential evidence of genomic inversions, translocations, and deletions in both the chromosomes. MAIN CONCLUSIONS The genome properties provide comprehensive information about the local isolates of Leptospira spp., and thereby, could facilitate the identification of new targets for the development of diagnostic kits and vaccines.
[Mh] Termos MeSH primário: Genoma Bacteriano
Leptospira interrogans/genética
Virulência/genética
Microbiologia da Água
[Mh] Termos MeSH secundário: Brasil
Leptospira interrogans/isolamento & purificação
Dados de Sequência Molecular
Filogenia
Polimorfismo Genético
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE


  4 / 647880 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28463807
[Au] Autor:Phillips C
[Ad] Endereço:Forensic Genetics Unit, Institute of Forensic Sciences, University of Santiago de Compostela, Galicia, Spain. Electronic address: c.phillips@mac.com.
[Ti] Título:A genomic audit of newly-adopted autosomal STRs for forensic identification.
[So] Source:Forensic Sci Int Genet;29:193-204, 2017 07.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In preparation for the growing use of massively parallel sequencing (MPS) technology to genotype forensic STRs, a comprehensive genomic audit of 73 STRs was made in 2016 [Parson et al., Forensic Sci. Int. Genet. 22, 54-63]. The loci examined included miniSTRs that were not in widespread use, but had been incorporated into MPS kits or were under consideration for this purpose. The current study expands the genomic analysis of autosomal STRs that are not commonly used, to include the full set of developed miniSTRs and an additional 24 STRs, most of which have been recently included in several supplementary forensic multiplex kits for capillary electrophoresis. The genomic audit of these 47 newly-adopted STRs examined the linkage status of new loci on the same chromosome as established forensic STRs; analyzed world-wide population variation of the newly-adopted STRs using published data; assessed their forensic informativeness; and compiled the sequence characteristics, repeat structures and flanking regions of each STR. A further 44 autosomal STRs developed for forensic analyses but not incorporated into commercial kits, are also briefly described.
[Mh] Termos MeSH primário: Impressões Digitais de DNA
Repetições de Microssatélites
[Mh] Termos MeSH secundário: Impressões Digitais de DNA/instrumentação
Ligação Genética
Genoma Humano
Seres Humanos
Dados de Sequência Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  5 / 647880 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28988126
[Au] Autor:Sopena S; Godoy C; Tabernero D; Homs M; Gregori J; Riveiro-Barciela M; Ruiz A; Esteban R; Buti M; Rodríguez-Frías F
[Ad] Endereço:Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, 28029 Madrid, Spain; Liver Pathology Unit, Departments of Biochemistry and Microbiology, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona (UAB), 08035 Barc
[Ti] Título:Quantitative characterization of hepatitis delta virus genome edition by next-generation sequencing.
[So] Source:Virus Res;243:52-59, 2018 01 02.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIM: To determine the capacity of next-generation sequencing (NGS) for quantifying edited and unedited HDV populations, and to confirm if edition is a general phenomenon taking place along the entire HDV region analyzed, as we previously reported (Homs M et al. PLoS One 2016, 11, e0158557). METHODS: Four serum samples from 4 patients with chronic HDV/HBV infection were included in the study. The region selected for analysis covered 360 nucleotides (nt), positions 910-1270 of the HDV genome, which included the HDAg ORF editing site (nt 1014 within codon 196). Quantification of edited and unedited genomes was performed by molecular cloning and Sanger sequencing and by NGS. To evaluate the reliability of the NGS values obtained, we combined a clone with an edited codon and one with an unedited codon in known percentages in a series of artificial mixtures, which were then analyzed by NGS. In addition, we determined the nt changes occurring over the complete amplified region after excluding the editing codon (196) to evaluate edition along it. RESULTS: In total, 11,208 quality-filtered sequences were obtained in the 4 samples. The 95% confidence intervals for the proportions of unedited populations by molecular cloning and NGS were overlapping, and those of cloning were wider, indicating that they are comparable and that NGS is more precise than cloning. Unedited genomes predominated over edited ones in all 4 samples analyzed by NGS and in 3 of the 4 samples analyzed by molecular cloning. In total, 83,276 quality-filtered sequences were obtained from the artificial mixtures. Percentages of the two viral populations detected by NGS in these mixtures were comparable to the expected percentages. Evaluation of edition along the HDV coding region showed that transitions were more frequent than transversions, accounting for 63.09% and 36.91%, respectively. Interestingly, among the 4 possible transition-type changes, G:A and A:G accounted for 73.86% of the total. CONCLUSION: Next-generation sequencing proved useful to quantify edited and unedited HDV genomes, and provided relevant information on the HDV quasispecies.
[Mh] Termos MeSH primário: Genoma Viral
Hepatite D/virologia
Vírus Delta da Hepatite/genética
Vírus Delta da Hepatite/isolamento & purificação
Sequenciamento de Nucleotídeos em Larga Escala/métodos
[Mh] Termos MeSH secundário: Sequência de Bases
Vírus Delta da Hepatite/classificação
Seres Humanos
Dados de Sequência Molecular
Filogenia
Replicação Viral
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


  6 / 647880 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29260551
[Au] Autor:Zhang L; Zhang H; Song Y
[Ad] Endereço:Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology , Zibo, 255049 Shandong, People's Republic of China.
[Ti] Título:Identification and Characterization of Diacylglycerol Acyltransferase from Oleaginous Fungus Mucor circinelloides.
[So] Source:J Agric Food Chem;66(3):674-681, 2018 Jan 24.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Acyl-CoA:diacylglycerol acyltransferase (DGAT) is a pivotal regulator of triacylglycerol (TAG) synthesis. The oleaginous fungus Mucor circinelloides has four putative DGATs: McDGAT1A, McDGAT1B, McDGAT2A, and McDGAT2B, classified into the DGAT1 and DGAT2 subfamilies, respectively. To identify and characterize DGATs in M. circinelloides, these four genes were expressed in Saccharomyces cerevisiae H1246 (TAG-deficient quadruple mutant), individually. TAG biosynthesis was restored only by the expression of McDGAT2B, and TAG content was significantly higher in the mutants with McDGAT2B expression than in a S. cerevisiae mutant with endogenous DGA1 expression. McDGAT2B prefers saturated fatty acids to monounsaturated fatty acids and has an obvious preference for C18:3 (ω-6) according to the results of substrate preference experiments. Furthermore, only the mRNA expression pattern of McDGAT2B correlated with TAG biosynthesis during a fermentation process. Our experiments strongly indicate that McDGAT2B is crucial for TAG accumulation, suggesting that it may be an essential target for metabolic engineering aimed at increasing lipid content of M. circinelloides.
[Mh] Termos MeSH primário: Diacilglicerol O-Aciltransferase/química
Proteínas Fúngicas/química
Mucor/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Diacilglicerol O-Aciltransferase/genética
Diacilglicerol O-Aciltransferase/metabolismo
Ácidos Graxos/química
Ácidos Graxos/metabolismo
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Dados de Sequência Molecular
Mucor/química
Mucor/genética
Família Multigênica
Alinhamento de Sequência
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Fungal Proteins); EC 2.3.1.20 (Diacylglycerol O-Acyltransferase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04295


  7 / 647880 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28471472
[Au] Autor:Panwalkar V; Neudecker P; Willbold D; Dingley AJ
[Ad] Endereço:ICS-6 (Strukturbiochemie), Forschungszentrum Jülich, Germany.
[Ti] Título:Multiple WW domains of Nedd4-1 undergo conformational exchange that is quenched upon peptide binding.
[So] Source:FEBS Lett;591(11):1573-1583, 2017 06.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The third WW domain (WW3*) of the ubiquitin ligase human neuronal precursor cell expressed developmentally downregulated gene 4-1 (hNedd4-1) was reported to bind its PY motif peptide by a coupled folding-binding equilibrium. However, it is unknown whether these thermodynamic properties are retained in the context of neighboring hNedd4-1 domains. In this report, NMR data show that the WW3* displays a fold-unfold equilibrium in the presence of neighboring WW domains, and that similar fold-unfold equilibria also likely exist for neighboring WW domains. These equilibria are quenched upon interaction with peptide. Thus, the binding mechanism of hNedd4-1 WW domains to proteins involves coupled folding and binding equilibria, and this mechanism may be a general feature that modulates peptide affinities of WW domains.
[Mh] Termos MeSH primário: Complexos Endossomais de Distribuição Requeridos para Transporte/química
Complexos Endossomais de Distribuição Requeridos para Transporte/metabolismo
Peptídeos/química
Peptídeos/metabolismo
Ubiquitina-Proteína Ligases/química
Ubiquitina-Proteína Ligases/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Seres Humanos
Espectroscopia de Ressonância Magnética
Dados de Sequência Molecular
Ubiquitina-Proteína Ligases Nedd4
Fases de Leitura Aberta/genética
Ligação Proteica
Domínios Proteicos
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Endosomal Sorting Complexes Required for Transport); 0 (Peptides); EC 2.3.2.26 (Nedd4 Ubiquitin Protein Ligases); EC 2.3.2.26 (Nedd4 protein, human); EC 2.3.2.27 (Ubiquitin-Protein Ligases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12664


  8 / 647880 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28463435
[Au] Autor:Peng T; Chen X; Pan Y; Zheng Z; Wei X; Xi J; Zhang J; Gao X; Shang Q
[Ad] Endereço:College of Plant Science, Jilin University, Changchun, China.
[Ti] Título:Transcription factor aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator is involved in regulation of the xenobiotic tolerance-related cytochrome P450 CYP6DA2 in Aphis gossypii Glover.
[So] Source:Insect Mol Biol;26(5):485-495, 2017 10.
[Is] ISSN:1365-2583
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The cotton aphid, Aphis gossypii, is one of the most economically important agricultural pests worldwide as it is polyphagous and resistant to many classes of insecticides. Overexpression of the cytochrome P450 monooxygenase (P450) CYP6DA2 has previously been found to be associated with gossypol and spirotetramat tolerance in the cotton aphid. In the present study, the elements located in the promoter region (-357:-343; -250:-241; -113:-104) of CYP6DA2 were shown to control promoter activity, and gossypol induction was observed. We hypothesized that the expression of CYP6DA2 is subject to transcriptional regulation. To investigate the underlying mechanism, we assessed two transcription factors, aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT), and found that the abundance of AhR was highly correlated with CYP6DA2 abundance. RNA interference of AhR or ARNT significantly decreased the levels of the target gene as well as those of its counterpart, and both dramatically repressed CYP6DA2 expression. Cotransfection of the ARNT, AhR, or AhR plus ARNT and CYP6DA2 promoter constructs elevated CYP6DA2 promoter activity, with the AhR plus ARNT cotransfection being the most effective. Thus, these elements located in the promoter were responsible for CYP6DA2 transcription, and CYP6DA2 expression was regulated by the transcription factors AhR and ARNT.
[Mh] Termos MeSH primário: Afídeos/metabolismo
Translocador Nuclear Receptor Aril Hidrocarboneto/metabolismo
Família 6 do Citocromo P450/metabolismo
Receptores de Hidrocarboneto Arílico/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Afídeos/genética
Compostos Aza
Sequência de Bases
Sequência Conservada
Família 6 do Citocromo P450/genética
Técnicas de Silenciamento de Genes
Gossipol
Proteínas de Insetos/metabolismo
Resistência a Inseticidas
Dados de Sequência Molecular
Regiões Promotoras Genéticas
Compostos de Espiro
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aza Compounds); 0 (Insect Proteins); 0 (Receptors, Aryl Hydrocarbon); 0 (Spiro Compounds); 138391-32-9 (Aryl Hydrocarbon Receptor Nuclear Translocator); 4G7KR034OX (spirotetramat); EC 1.14.14.1 (Cytochrome P450 Family 6); KAV15B369O (Gossypol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1111/imb.12311


  9 / 647880 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29175332
[Au] Autor:Kozlov G; Wong K; Wang W; Skubák P; Muñoz-Escobar J; Liu Y; Siddiqui N; Pannu NS; Gehring K
[Ad] Endereço:Department of Biochemistry, Groupe de recherche axé sur la structure des protéines, McGill University, Montreal, QC H3G 0B1, Canada.
[Ti] Título:Ankyrin repeats as a dimerization module.
[So] Source:Biochem Biophys Res Commun;495(1):1002-1007, 2018 01 01.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Legionella pneumophila is a pathogen, causing severe pneumonia in humans called Legionnaires' disease. AnkC (LegA12) is a poorly characterized 495-residue effector protein conserved in multiple Legionella species. Here, we report the crystal structure of a C-terminally truncated AnkC (2-384) at 3.2 Å resolution. The structure shows seven ankyrin repeats (ARs) with unique structural features. AnkC forms a dimer along the outer surface of loops between ARs. The dimer exists both in the crystal form and in solution, as shown by analytical ultracentrifugation. This is the first example of ARs as a dimerization module as opposed to solely a protein interaction domain. In addition, a novel α-helix insert between AR3-AR4 is positioned across the surface opposite the ankyrin groove. Sequence conservation suggests that the ankyrin groove of AnkC is a functional site that interacts with binding targets. This ankyrin domain structure is an important step towards a functional characterization of AnkC.
[Mh] Termos MeSH primário: Repetição de Anquirina
Anquirinas/química
Anquirinas/ultraestrutura
Modelos Químicos
Modelos Moleculares
Multimerização Proteica
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sítios de Ligação
Simulação por Computador
Sequência Conservada
Legionella pneumophila/metabolismo
Dados de Sequência Molecular
Ligação Proteica
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ankyrins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  10 / 647880 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27771561
[Au] Autor:Grace M; Munger K
[Ad] Endereço:Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, 150 Harrison Avenue, Jaharis 607, Boston, MA, 02111, USA.
[Ti] Título:Proteomic analysis of the gamma human papillomavirus type 197 E6 and E7 associated cellular proteins.
[So] Source:Virology;500:71-81, 2017 01.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gamma HPV197 was the most frequently identified HPV when human skin cancer specimens were analyzed by deep sequencing (Arroyo Muhr et al., Int. J. Cancer 136: 2546-55, 2015). To gain insight into the biological activities of HPV197, we investigated the cellular interactomes of HPV197 E6 and E7. HPV197 E6 protein interacts with a broad spectrum of cellular LXXLL domain proteins, including UBE3A and MAML1. HPV197 E6 also binds and inhibits the TP53 tumor suppressor and interacts with the CCR4-NOT ubiquitin ligase and deadenylation complex. Despite lacking a canonical retinoblastoma (RB1) tumor suppressor binding site, HPV197 E7 binds RB1 and activates E2F transcription. Hence, HPV197 E6 and E7 proteins interact with a similar set of cellular proteins as E6 and E7 proteins encoded by HPVs that have been linked to human carcinogenesis and/or have transforming activities in vitro.
[Mh] Termos MeSH primário: Gammapapillomavirus/metabolismo
Proteínas Oncogênicas Virais/metabolismo
Proteínas E7 de Papillomavirus/metabolismo
Infecções por Papillomavirus/virologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Ligação a DNA/genética
Proteínas de Ligação a DNA/metabolismo
Gammapapillomavirus/química
Gammapapillomavirus/classificação
Gammapapillomavirus/genética
Seres Humanos
Dados de Sequência Molecular
Proteínas Oncogênicas Virais/química
Proteínas Oncogênicas Virais/genética
Proteínas E7 de Papillomavirus/química
Proteínas E7 de Papillomavirus/genética
Infecções por Papillomavirus/genética
Infecções por Papillomavirus/metabolismo
Ligação Proteica
Proteômica
Alinhamento de Sequência
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
Ubiquitina-Proteína Ligases/genética
Ubiquitina-Proteína Ligases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (MAML1 protein, human); 0 (Oncogene Proteins, Viral); 0 (Papillomavirus E7 Proteins); 0 (Transcription Factors); EC 2.3.2.26 (UBE3A protein, human); EC 2.3.2.27 (Ubiquitin-Protein Ligases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE



página 1 de 64788 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde