Base de dados : MEDLINE
Pesquisa : L01.737.787 [Categoria DeCS]
Referências encontradas : 2148 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 215 ir para página                         

  1 / 2148 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29402038
[Au] Autor:Youn Y; Ishitsuka Y; Jin C; Selvin PR
[Ti] Título:Thermal nanoimprint lithography for drift correction in super-resolution fluorescence microscopy.
[So] Source:Opt Express;26(2):1670-1680, 2018 Jan 22.
[Is] ISSN:1094-4087
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Localization-based super-resolution microscopy enables imaging of biological structures with sub-diffraction-limited accuracy, but generally requires extended acquisition time. Consequently, stage drift often limits the spatial precision. Previously, we reported a simple method to correct for this by creating an array of 1 µm fiducial markers, every ~8 µm, on the coverslip, using UV-nanoimprint lithography (UV-NIL). While this allowed reliable and accurate 3D drift correction, it suffered high autofluorescence background with shorter wavelength illumination, unstable adsorption to the substrate glass surface, and suboptimal biocompatibility. Here, we present an improved fiducial micro-pattern prepared by thermal nanoimprint lithography (T-NIL). The new pattern is made of a thermal plastic material with low fluorescence backgrounds across the wide excitation range, particularly in the blue-region; robust structural stability under cell culturing condition; and a high bio-compatibility in terms of cell viability and adhesion. We demonstrate drift precision to 1.5 nm for lateral (x, y) and 6.1 nm axial (z) axes every 0.2 seconds for a total of 1 min long image acquisition. As a proof of principle, we acquired 4-color wide-field fluorescence images of live mammalian cells; we also acquired super-resolution images of fixed hippocampal neurons, and super-resolution images of live glutamate receptors and postsynaptic density proteins.
[Mh] Termos MeSH primário: Marcadores Fiduciais
Aumento da Imagem/métodos
Microscopia de Fluorescência/métodos
Nanotecnologia
Neurônios
Impressão
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis
Fluorescência
Células HeLa
Hipocampo/citologia
Seres Humanos
Neuroglia
Polímeros
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Polymers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE
[do] DOI:10.1364/OE.26.001670


  2 / 2148 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28453952
[Au] Autor:Perea M; Rosa E; Marcet A
[Ad] Endereço:Universitat de València, Valencia, Spain. Electronic address: mperea@uv.es.
[Ti] Título:Where is the locus of the lowercase advantage during sentence reading?
[So] Source:Acta Psychol (Amst);177:30-35, 2017 Jun.
[Is] ISSN:1873-6297
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:While most models of visual word identification and reading posit that a word's visual codes are rapidly transformed onto case-invariant representations (i.e., table and TABLE would equally activate the word unit corresponding to "table"), a number of experiments have shown a lowercase advantage in various word identification and reading tasks. In the present experiment, we examined the locus of this lowercase advantage by comparing the pattern of eye movements when reading sentences in lowercase vs. uppercase. Each sentence contained a target word that was high or low in word-frequency. Overall, results showed faster reading times for lowercase than for uppercase sentences. More important, while the word-frequency effect was sizeable in the first-fixation durations on the target word, the lowercase advantage only arose in the gaze durations (i.e., the sum of durations of first-pass fixations on the target word, including refixations). Furthermore, we found an effect of word-frequency, but not of letter case, in the first-fixation duration on target words with multiple first-pass fixations. Taken together, these findings suggest that the lowercase advantage reflects operations that do not occur in the initial contact with the lexical entries.
[Mh] Termos MeSH primário: Compreensão/fisiologia
Impressão
Leitura
Semântica
[Mh] Termos MeSH secundário: Adulto
Movimentos Oculares/fisiologia
Feminino
Fixação Ocular/fisiologia
Seres Humanos
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171222
[Lr] Data última revisão:
171222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  3 / 2148 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28737607
[Au] Autor:Alabdulkader B; Leat SJ
[Ad] Endereço:School of Optometry and Vision Science, University of Waterloo, Waterloo, Ontario, Canada.
[Ti] Título:A Standardized Arabic Reading Acuity Chart: The Balsam Alabdulkader-Leat Chart.
[So] Source:Optom Vis Sci;94(8):807-816, 2017 Aug.
[Is] ISSN:1538-9235
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The aim of this study was to develop and validate the first standardized Arabic continuous text near-visual-acuity chart, the Balsam Alabdulkader-Leat (BAL) chart. METHODS: Three versions of the BAL chart were created from previously validated sentences. Reading acuity (RA) and reading speed in standard-length words per minute (SLWPM) were measured for three versions of the BAL chart and three English charts (MNREAD, Colenbrander, and Radner) for 86 bilingual adults with normal vision aged 15 to 59 years. The RA and SLWPM were compared using analysis of variance. To analyze agreement between the charts, Bland-Altman plots were used. Normal visual acuity (0.00 logMAR [log minimum angle of resolution]) was calibrated for the BAL chart with linear regression analysis. RESULTS: Average RAs for BAL1, BAL2, and BAL3 were 0.62, 0.64 and 0.65 log-point print, respectively, which were statistically significantly different (repeated-measures analysis of variance, P < .05), but not considered clinically significant. The coefficients of agreement for RA between the BAL charts were 0.054 (between 1 and 2), 0.061 (between 2 and 3), and 0.059 (between 1 and 3). Linear regression between the average RA for the BAL chart and the MNREAD and Radner charts showed that 0.7 log-point size at 40 cm is equivalent to 0.00 logMAR, and the new BAL chart was labeled accordingly. Mean SLWPM for the BAL charts was 201, 195, and 195 SLWPM, respectively, and for the Colenbrander, MNREAD, and Radner charts was 146, 171, and 146, respectively. The coefficients of agreement for log-SLWPM between BAL1 and BAL2, BAL2 and BAL3, and BAL1 and BAL3 were 0.063, 0.064, and 0.057 log-SLWPM, respectively. CONCLUSIONS: The BAL chart showed high interchart agreement. It is recommended for accurate near performance measures in Arabic for both research and clinical settings.
[Mh] Termos MeSH primário: Árabes
Linguagem
Leitura
Testes Visuais/métodos
Acuidade Visual/fisiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Feminino
Seres Humanos
Masculino
Meia-Idade
Impressão
Reprodutibilidade dos Testes
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE
[do] DOI:10.1097/OPX.0000000000001103


  4 / 2148 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28692846
[Au] Autor:Tallapragada SD; Layek K; Mukherjee R; Mistry KK; Ghosh M
[Ad] Endereço:National Institute of Technology, M. G. Avenue, Durgapur 713209, India.
[Ti] Título:Development of screen-printed electrode based immunosensor for the detection of HER2 antigen in human serum samples.
[So] Source:Bioelectrochemistry;118:25-30, 2017 Dec.
[Is] ISSN:1878-562X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, an immunosensor based on screen-printed electrode (SPE) has been developed for the detection of Human Epidermal Growth Factor Receptor-2 (HER2) antigen. The SPEs were fabricated and a sandwich enzyme linked immunosorbent assay (ELISA) format was followed for the construction of the immunosensor. The capture antibody (mouse anti-human ErbB2) was coated onto the electrode surface without any prior surface modification, followed by the addition of recombinant human HER2 antigen. Biotinylated goat anti-human ErbB2 was used as the detection antibody which was linked to streptavidin conjugated horseradish peroxidase (HRP). 3,3',5,5'-tetramethylbenzidine (TMB) was used as the substrate. The redox reaction was measured using cyclic voltammetry at scan rate of 50mV/s for the quantification of the antigen in solution. The biotin-avidin chemistry enabled the accurate detection of the antigen in nanogram levels. The amperometric signal obtained increased linearly with increase in the HER2 concentration and two-fold linear range was obtained between 5ng/ml-20ng/ml and 20-200ng/ml respectively. The limit of detection (LOD) and the limit of quantification (LOQ) of this immunosensor were found to be 4ng/ml and 5ng/ml respectively. The detection of HER2 in the serum samples of invasive and non-invasive breast cancer patients has been realized.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/instrumentação
Análise Química do Sangue/instrumentação
Ensaio de Imunoadsorção Enzimática/instrumentação
Impressão
Receptor ErbB-2/sangue
[Mh] Termos MeSH secundário: Anticorpos Imobilizados/química
Anticorpos Imobilizados/imunologia
Benzidinas/química
Eletroquímica
Eletrodos
Peroxidase do Rábano Silvestre/química
Peroxidase do Rábano Silvestre/metabolismo
Seres Humanos
Limite de Detecção
Receptor ErbB-2/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Immobilized); 0 (Benzidines); 3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine); EC 1.11.1.- (Horseradish Peroxidase); EC 2.7.10.1 (ERBB2 protein, human); EC 2.7.10.1 (Receptor, ErbB-2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE


  5 / 2148 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28524743
[Au] Autor:Pirela SV; Martin J; Bello D; Demokritou P
[Ad] Endereço:a Department of Environmental Health, Harvard T.H. Chan School of Public Health , Center for Nanotechnology and Nanotoxicology , Boston , MA , USA.
[Ti] Título:Nanoparticle exposures from nano-enabled toner-based printing equipment and human health: state of science and future research needs.
[So] Source:Crit Rev Toxicol;47(8):678-704, 2017 Sep.
[Is] ISSN:1547-6898
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Toner formulations used by laser printers (LP) and photocopiers (PC), collectively called "toner-based printing equipment" (TPE), are nano-enabled products (NEP) because they contain several engineered nanomaterials (ENM) that improve toner performance. It has been shown that during consumer use (printing), these ENM are released in the air, together with other semi-volatile organic nanoparticles, and newly formed gaseous co-pollutants such as volatile organic compounds (VOC). The aim of this review is to detail and analyze physico-chemical and morphological (PCM), as well as the toxicological properties of particulate matter (PM) emissions from TPE. The review covers evolution of science since the early 2000, when this printing technology first became a subject of public interest, as well as the lagging regulatory framework around it. Important studies that have significantly changed our understanding of these exposures are also highlighted. The review continues with a critical appraisal of the most up-to-date cellular, animal and human toxicological evidence on the potential adverse human health effects of PM emitted from TPE. We highlight several limitations of existing studies, including (i) use of high and often unrealistic doses in vitro or in vivo; (ii) unrealistically high-dose rates in intratracheal instillation studies; (iii) improper use of toners as surrogate for emitted nanoparticles; (iv) lack of or inadequate PCM characterization of exposures; and (v) lack of dosimetry considerations in in vitro studies. Presently, there is compelling evidence that the PM from TPE are biologically active and capable of inducing oxidative stress in vitro and in vivo, respiratory tract inflammation in vivo (in rats) and in humans, several endpoints of cellular injury in monocultures and co-cultures, including moderate epigenetic modifications in vitro. In humans, limited epidemiological studies report typically 2-3 times higher prevalence of chronic cough, wheezing, nasal blockage, excessive sputum production, breathing difficulties, and shortness of breath, in copier operators relative to controls. Such symptoms can be exacerbated during chronic exposures, and in individuals susceptible to inhaled pollutants. Thus respiratory, immunological, cardiovascular, and other disorders may be developed following such exposures; however, further toxicological and larger scale molecular epidemiological studies must be done to fully understand the mechanism of action of these TPE emitted nanoparticles. Major research gaps have also been identified. Among them, a methodical risk assessment based on "real world" exposures rather than on the toner particles alone needs to be performed to provide the much-needed data to establish regulatory guidelines protective of individuals exposed to TPE emissions at both the occupational and consumer level. Industry-wide molecular epidemiology as well as mechanistic animal and human studies are also urgently needed.
[Mh] Termos MeSH primário: Exposição Ocupacional
Impressão
[Mh] Termos MeSH secundário: Poluentes Atmosféricos/toxicidade
Animais
Seres Humanos
Nanopartículas/toxicidade
Material Particulado/toxicidade
Pesquisa/tendências
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Particulate Matter)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE
[do] DOI:10.1080/10408444.2017.1318354


  6 / 2148 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28425993
[Au] Autor:Ziemelis K
[Ti] Título:Materials science: How to print glass.
[So] Source:Nature;544(7650):305, 2017 04 19.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Vidro
Impressão
[Mh] Termos MeSH secundário: Teste de Materiais
Ciência
[Pt] Tipo de publicação:NEWS; COMMENT
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1038/544305a


  7 / 2148 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28411527
[Au] Autor:Centanni TM; King LW; Eddy MD; Whitfield-Gabrieli S; Gabrieli JDE
[Ad] Endereço:Massachusetts Institute of Technology, McGovern Institute for Brain Research and Department of Brain and Cognitive Sciences, 43 Vassar St., 46-4033 Cambridge, MA 02139, USA. Electronic address: tmcentanni@gmail.com.
[Ti] Título:Development of sensitivity versus specificity for print in the visual word form area.
[So] Source:Brain Lang;170:62-70, 2017 Jul.
[Is] ISSN:1090-2155
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:An area near the left lateral occipito-temporal sulcus that responds preferentially to print has been designated as the visual word form area (VWFA). Research suggests that specialization in this brain region increases as reading expertise is achieved. Here we aimed to characterize that development in terms of sensitivity (response to printed words relative to non-linguistic faces) versus specificity (response to printed words versus line drawings of nameable objects) in typically reading children ages 7-14 versus young adults as measured by functional magnetic resonance imaging (fMRI). Relative to adults, children displayed equivalent sensitivity but reduced specificity. These findings suggest that sensitivity for print relative to non-linguistic stimuli develops relatively early in the VWFA in the course of reading development, but that specificity for printed words in VWFA is still developing through at least age 14.
[Mh] Termos MeSH primário: Desenvolvimento da Linguagem
Impressão
Leitura
Lobo Temporal/fisiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Mapeamento Encefálico
Criança
Face
Feminino
Seres Humanos
Imagem por Ressonância Magnética
Masculino
Estimulação Luminosa
Percepção Visual/fisiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE


  8 / 2148 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28391101
[Au] Autor:Bravo D; Ferrero P; Penya-Roja JM; Álvarez-Hornos FJ; Gabaldón C
[Ad] Endereço:Research Group GI(2)AM, Department of Chemical Engineering, University of Valencia, Avd. Universitat s/n, 46100, Burjassot, Spain; Pure Air Solutions, PB 135, 8440 AC, Heerenveen, The Netherlands.
[Ti] Título:Control of VOCs from printing press air emissions by anaerobic bioscrubber: Performance and microbial community of an on-site pilot unit.
[So] Source:J Environ Manage;197:287-295, 2017 Jul 15.
[Is] ISSN:1095-8630
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel process consisted of an anaerobic bioscrubber was studied at the field scale for the removal of volatile organic compounds (VOCs) emitted from a printing press facility. The pilot unit worked under high fluctuating waste gas emissions containing ethanol, ethyl acetate, and 1-ethoxy-2-propanol as main pollutants, with airflows ranging between 184 and 1253 m h and an average concentration of 1126 ± 470 mg-C Nm . Three scrubber configurations (cross-flow and vertical-flow packings and spray tower) were tested, and cross-flow packing was found to be the best one. For this packing, daily average values of VOC removal efficiency ranged between 83% and 93% for liquid to air volume ratios between 3.5·10 and 9.1·10 . Biomass growth was prevented by periodical chemical cleaning; the average pressure drop was 165 Pa m . Rapid initiation of anaerobic degradation was achieved by using granular sludge from a brewery wastewater treatment plant. Despite the intermittent and fluctuating organic load, the expanded granular sludge bed reactor showed an excellent level of performance, reaching removal efficiencies of 93 ± 5% at 25.1 ± 3.2 °C, with biogas methane content of 94 ± 3% in volume. Volatile fatty acid concentration was as low as 200 mg acetic acid L by treating daily average organic loads up to 3.0 kg COD h , equivalent to 24 kg COD m bed d . The denaturing gradient gel electrophoresis (DGGE) results revealed the initial shift of the domains Archaea and Bacteria associated with the limitation of the carbon source to a few organic solvents. The Archaea domain was more sensitive, resulting in a drop of the Shannon index from 1.07 to 0.41 in the first 123 days. Among Archaea, the predominance of Methanosaeta persisted throughout the experimental period. The increase in the proportion of Methanospirillum and Methanobacterium sp. was linked to the spontaneous variations of operating temperature and load, respectively. Among Bacteria, high levels of ethanol degraders (Geobacter and Pelobacter sp.) were observed during the trial.
[Mh] Termos MeSH primário: Reatores Biológicos
Impressão
Compostos Orgânicos Voláteis
Eliminação de Resíduos Líquidos
[Mh] Termos MeSH secundário: Anaerobiose
Metano
Pilotos
Esgotos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sewage); 0 (Volatile Organic Compounds); OP0UW79H66 (Methane)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170410
[St] Status:MEDLINE


  9 / 2148 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28379948
[Au] Autor:Kim YD; Hone J
[Ad] Endereço:Department of Physics and Center for Humanities and Sciences, Kyung Hee University, Seoul 02447, Republic of Korea.
[Ti] Título:Materials science: Screen printing of 2D semiconductors.
[So] Source:Nature;544(7649):167-168, 2017 04 13.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Impressão
Semicondutores
[Mh] Termos MeSH secundário: Ciência
[Pt] Tipo de publicação:NEWS; COMMENT
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1038/nature21908


  10 / 2148 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28327370
[Au] Autor:Curzietti M; Bonnefond A; Staub B; Vidailhet P; Doignon-Camus N
[Ad] Endereço:INSERM 1114, Department of Psychiatry, University of Strasbourg, France. Electronic address: maxime.curzietti@orange.fr.
[Ti] Título:The effects of age on visual expertise for print.
[So] Source:Brain Lang;169:48-56, 2017 Jun.
[Is] ISSN:1090-2155
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Progressive visual processing decline is a known factor in aging. The present study investigates the evolution of visual expertise for printed stimuli with aging. Fifty-five participants of increasing age (20-30, 40-50, 60-70, 75-85years old) were recruited. Behavioral and EEG data were collected during a lexical decision task, in which words and symbol strings were presented. Analyses of EEG data focused mainly on three major points: visual expertise for print, automatization of the expertise and differences in attentional demand between the processing of words and symbols. Results indicated a preservation of visual expertise with age, with larger N170 amplitude for words than for symbols. Moreover, a decrease in stimulus processing speed was observed as a function of age. No difference in attentional demand as a function of stimulus was observed.
[Mh] Termos MeSH primário: Envelhecimento/fisiologia
Impressão
Leitura
Percepção Visual/fisiologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Atenção/fisiologia
Eletroencefalografia
Potenciais Evocados
Feminino
Seres Humanos
Masculino
Meia-Idade
Estimulação Luminosa
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE



página 1 de 215 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde