Base de dados : MEDLINE
Pesquisa : N06.230.150 [Categoria DeCS]
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[PMID]:29431936
[Au] Autor:Gildenskiold RS; Tatyanyuk TK; Savelyev SI; Rekis VK
[Ti] Título:[The possibility of provision of hygienically safe residing for the population in residential construction located closely to air transport enterprises].
[So] Source:Gig Sanit;95(11):1029-32, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Operation of the modern heavy aircraft on the surrounding vast territory is associated with the appearance of high levels of sound pressure generated by the powerful engines, especially during takeoff and landing operations. Currently, the elimination or significant reduction of noise pollution on the environment technically does not yet have a radical solution and the possible reduction of the impact ofnoise pollution on the residents ofnearby settlements is achievedfor residents of building by the optimal location of the runway, changing in conditions for the takeoff and landing of aircrafts, the ordering of the regimen of the airport, the introduction of the package sufficiently effective anti-noise diverse - engineering, planning activities, in some cases, the creation ofprotective noise shield on the border of the residential area and the construction ofprotective awnings over the children's and sports fields with fencing shields converted in the direction of the runway. An example of a positive decision, taking into account the complexity and variety of aspects of the problem, it is possible to consider the development of the project plan of the new microdistrict «Eletsky¼ in the Soviet district of the city of Lipetsk, falling under the impact of aircraft noise of plying aircrafts of the military airfield.
[Mh] Termos MeSH primário: Aviação
Planejamento em Saúde/organização & administração
Habitação
Ruído/prevenção & controle
[Mh] Termos MeSH secundário: Aeronaves
Aeroportos/normas
Aviação/métodos
Aviação/organização & administração
Ambiente Controlado
Habitação/organização & administração
Habitação/normas
Seres Humanos
Desenvolvimento Industrial
Ruído dos Transportes/efeitos adversos
Ruído dos Transportes/prevenção & controle
Saúde Pública/métodos
Federação Russa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


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[PMID]:29106842
[Au] Autor:Markel TA; Gormley T; Greeley D; Ostojic J; Wise A; Rajala J; Bharadwaj R; Wagner J
[Ad] Endereço:Department of Surgery, Riley Hospital for Children, Indiana University Health, Indianapolis, IN. Electronic address: tmarkel@iupui.edu.
[Ti] Título:Hats Off: A Study of Different Operating Room Headgear Assessed by Environmental Quality Indicators.
[So] Source:J Am Coll Surg;225(5):573-581, 2017 Nov.
[Is] ISSN:1879-1190
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The effectiveness of operating room headgear in preventing airborne contamination has been called into question. We hypothesized that bouffant style hats would be as effective in preventing bacterial and particulate contamination in the operating room compared with disposable or cloth skull caps, and bouffant style hats would have similar permeability, particle penetration, and porosity compared with skull caps. STUDY DESIGN: Disposable bouffant and skull cap hats and newly laundered cloth skull caps were tested. A mock surgical procedure was used in a dynamic operating room environment. Airborne particulate and microbial contaminants were sampled. Hat fabric was tested for permeability, particle transmission, and pore sizes. RESULTS: No significant differences were observed between disposable bouffant and disposable skull caps with regard to particle or actively sampled microbial contamination. However, when compared with disposable skull caps, disposable bouffant hats did have significantly higher microbial shed at the sterile field, as measured by passive settle plate analysis (p < 0.05). When compared with cloth skull caps, disposable bouffants yielded higher levels of 0.5 µm and 1.0 µm particles and significantly higher microbial shed detected with passive analysis. Fabric assessment determined that disposable bouffant hats had larger average and maximum pore sizes compared with cloth skull caps, and were significantly more permeable than either disposable or cloth skull caps. CONCLUSIONS: Disposable bouffant hats had greater permeability, penetration, and greater microbial shed, as assessed by passive microbial analysis compared with disposable skull caps. When compared with cloth skull caps, disposable bouffants yielded greater permeability, greater particulate contamination, and greater passive microbial shed. Disposable style bouffant hats should not be considered superior to skull caps in preventing airborne contamination in the operating room.
[Mh] Termos MeSH primário: Equipamentos Descartáveis/provisão & distribuição
Ambiente Controlado
Salas Cirúrgicas/normas
Têxteis/normas
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171107
[St] Status:MEDLINE


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[PMID]:28922412
[Au] Autor:Okubo T; Osaki T; Nozaki E; Uemura A; Sakai K; Matushita M; Matsuo J; Nakamura S; Kamiya S; Yamaguchi H
[Ad] Endereço:Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Kita-ku, Sapporo, Japan.
[Ti] Título:Walker occupancy has an impact on changing airborne bacterial communities in an underground pedestrian space, as small-dust particles increased with raising both temperature and humidity.
[So] Source:PLoS One;12(9):e0184980, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although human occupancy is a source of airborne bacteria, the role of walkers on bacterial communities in built environments is poorly understood. Therefore, we visualized the impact of walker occupancy combined with other factors (temperature, humidity, atmospheric pressure, dust particles) on airborne bacterial features in the Sapporo underground pedestrian space in Sapporo, Japan. Air samples (n = 18; 4,800L/each sample) were collected at 8:00 h to 20:00 h on 3 days (regular sampling) and at early morning / late night (5:50 h to 7:50 h / 22:15 h to 24:45 h) on a day (baseline sampling), and the number of CFUs (colony forming units) OTUs (operational taxonomic units) and other factors were determined. The results revealed that temperature, humidity, and atmospheric pressure changed with weather. The number of walkers increased greatly in the morning and evening on each regular sampling day, although total walker numbers did not differ significantly among regular sampling days. A slight increase in small dust particles (0.3-0.5µm) was observed on the days with higher temperature regardless of regular or baseline sampling. At the period on regular sampling, CFU levels varied irregularly among days, and the OTUs of 22-phylum types were observed, with the majority being from Firmicutes or Proteobacteria (γ-), including Staphylococcus sp. derived from human individuals. The data obtained from regular samplings reveled that although no direct interaction of walker occupancy and airborne CFU and OTU features was observed upon Pearson's correlation analysis, cluster analysis indicated an obvious lineage consisting of walker occupancy, CFU numbers, OTU types, small dust particles, and seasonal factors (including temperature and humidity). Meanwhile, at the period on baseline sampling both walker and CFU numbers were similarly minimal. Taken together, the results revealed a positive correlation of walker occupancy with airborne bacteria that increased with increases in temperature and humidity in the presence of airborne small particles. Moreover, the results indicated that small dust particles at high temperature and humidity may be a crucial factor responsible for stabilizing the bacteria released from walkers in built environments. The findings presented herein advance our knowledge and understanding of the relationship between humans and bacterial communities in built environments, and will help improve public health in urban communities.
[Mh] Termos MeSH primário: Ambiente Controlado
Firmicutes
Temperatura Alta
Umidade
Material Particulado/análise
Proteobactérias
Caminhada
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Japão
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Particulate Matter)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184980


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[PMID]:28768783
[Au] Autor:Refaie R; Rushton P; McGovern P; Thompson D; Serrano-Pedraza I; Rankin KS; Reed M
[Ad] Endereço:Northumbria Healthcare NHS Foundation Trust, Woodhorn Ln, Ashington, Northumberland NE63 9JJ, UK.
[Ti] Título:The effect of operating lights on laminar flow: an experimental study using neutrally buoyant helium bubbles.
[So] Source:Bone Joint J;99-B(8):1061-1066, 2017 Aug.
[Is] ISSN:2049-4408
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: The interaction between surgical lighting and laminar airflow is poorly understood. We undertook an experiment to identify any effect contemporary surgical lights have on laminar flow and recommend practical strategies to limit any negative effects. MATERIALS AND METHODS: Neutrally buoyant bubbles were introduced into the surgical field of a simulated setup for a routine total knee arthroplasty in a laminar flow theatre. Patterns of airflow were observed and the number of bubbles remaining above the surgical field over time identified. Five different lighting configurations were assessed. Data were analysed using simple linear regression after logarithmic transformation. RESULTS: In the absence of surgical lights, laminar airflow was observed, bubbles were cleared rapidly and did not accumulate. If lights were placed above the surgical field laminar airflow was abolished and bubbles rose from the surgical field to the lights then circulated back to the surgical field. The value of the decay parameter (slope) of the two setups differed significantly; no light (b = -1.589) one light (b = -0.1273, p < 0.001). Two lights touching (b = -0.1191) above the surgical field had a similar effect to that of a single light (p = 0. 2719). Two lights positioned by arms outstretched had a similar effect (b = -0.1204) to two lights touching (p = 0.998) and one light (p = 0.444). When lights were separated widely (160 cm), laminar airflow was observed but the rate of clearance of the bubbles remained slower (b = -1.1165) than with no lights present (p = 0.004). CONCLUSION: Surgical lights have a significantly negative effect on laminar airflow. Lights should be positioned as far away as practicable from the surgical field to limit this effect. Cite this article: 2017;99-B:1061-6.
[Mh] Termos MeSH primário: Artroplastia do Joelho/métodos
Desinfecção/métodos
Hélio/farmacologia
Iluminação/métodos
Salas Cirúrgicas
Infecção da Ferida Cirúrgica/prevenção & controle
[Mh] Termos MeSH secundário: Ambiente Controlado
Seres Humanos
Ventilação/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
206GF3GB41 (Helium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1302/0301-620X.99B8.BJJ-2016-0581.R2


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[PMID]:28644860
[Au] Autor:Feldman MJ; Paul RE; Banan D; Barrett JF; Sebastian J; Yee MC; Jiang H; Lipka AE; Brutnell TP; Dinneny JR; Leakey ADB; Baxter I
[Ad] Endereço:Donald Danforth Plant Science Center, St. Louis, Missouri, United States of America.
[Ti] Título:Time dependent genetic analysis links field and controlled environment phenotypes in the model C4 grass Setaria.
[So] Source:PLoS Genet;13(6):e1006841, 2017 Jun.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vertical growth of plants is a dynamic process that is influenced by genetic and environmental factors and has a pronounced effect on overall plant architecture and biomass composition. We have performed six controlled growth trials of an interspecific Setaria italica x Setaria viridis recombinant inbred line population to assess how the genetic architecture of plant height is influenced by developmental queues, water availability and planting density. The non-destructive nature of plant height measurements has enabled us to monitor height throughout the plant life cycle in both field and controlled environments. We find that plant height is reduced under water limitation and high density planting and affected by growth environment (field vs. growth chamber). The results support a model where plant height is a heritable, polygenic trait and that the major genetic loci that influence plant height function independent of growth environment. The identity and contribution of loci that influence height changes dynamically throughout development and the reduction of growth observed in water limited environments is a consequence of delayed progression through the genetic program which establishes plant height in Setaria. In this population, alleles inherited from the weedy S. viridis parent act to increase plant height early, whereas a larger number of small effect alleles inherited from the domesticated S. italica parent collectively act to increase plant height later in development.
[Mh] Termos MeSH primário: Ambiente Controlado
Locos de Características Quantitativas/genética
Setaria (Planta)/genética
[Mh] Termos MeSH secundário: Alelos
Biomassa
Mapeamento Cromossômico
Genoma de Planta
Genótipo
Herança Multifatorial/genética
Fenótipo
Setaria (Planta)/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170624
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006841


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[PMID]:28557780
[Au] Autor:Mixon W; Roth A
[Ad] Endereço:The Compounding Pharmacy, Hickory, North Carolina. wmixon@thecompoundingrx.com.
[Ti] Título:Microbiologic Testing for 503A Sterile-Compounding Pharmacies.
[So] Source:Int J Pharm Compd;21(3):193-203, 2017 May-Jun.
[Is] ISSN:1092-4221
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Compounding pharmacists must ensure that the sterile preparations they dispense are free of microbiologic contamination. Working in a cleanroom under controlled conditions (proper differential air pressure, temperature, and humidity; acceptable levels of viable and nonviable airborne particles and surface counts, etc.) and testing the efficacy of cleaning and disinfecting practices via environmental monitoring (viable-air and surface testing, glove-fingertip-thumb testing, etc.) are essential to preparing contamination-free medications. Sterile-compounding pharmacists must understand how to monitor their cleanroom environment and, if they perform testing in house, to interpret the results of simple microbiologic tests (a skill helpful even when tests are outsourced to a contract laboratory). In this article, which pertains to 503A sterile compounding, and is based on the current version of United States Pharmacopeia (USP) Chapter <797>, basic concepts in microbiology and the microbial tests that can be performed and interpreted in house and those that must be outsourced are discussed. Streamlining communication with contract laboratory personnel is reviewed. Requirements for an inhouse microbiology laboratory are presented, and the advantages and disadvantages of inhouse and outsourced testing are examined. A list of suggested reading is provided for easy reference. In a subsequent article, environmental monitoring and analysis will be addressed in detail.
[Mh] Termos MeSH primário: Composição de Medicamentos/normas
Farmácias/normas
Esterilização/normas
[Mh] Termos MeSH secundário: Desinfetantes/normas
Desinfecção/normas
Contaminação de Medicamentos/prevenção & controle
Ambiente Controlado
Monitoramento Ambiental/normas
Seres Humanos
Farmacêuticos/normas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disinfectants)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE


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[PMID]:28542169
[Au] Autor:Wang X; Zhang D; Guan B; Qi Q; Tong S
[Ad] Endereço:Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, P. R. China.
[Ti] Título:Optimum water supplement strategy to restore reed wetland in the Yellow River Delta.
[So] Source:PLoS One;12(5):e0177692, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In order to supply optimum water to restore reed wetlands used for bird habitats, a field investigation and greenhouse experiment were conducted. Three water supplementation stages (early stage at 20 May, middle stage at 20 July and later stage at 20 September, respectively) and five depths (0, 10, 15, 20 and 35 cm over the surface, respectively) were established, with three replicates for each treatment combination. Reed growth characteristics (survival rate, height, density and biomass) and soil properties of field investigation and experiment were recorded to determine the impacts of water supplementation on reed wetland restoration. The field investigation showed that reeds in natural wetlands grow better than those in degraded wetlands and soil properties in degraded wetlands were significantly different from soils in natural wetlands. With freshwater supplementation, reed growth characteristics and soil properties greatly improved. As water depth increased, reed growth decreased gradually. Reeds grew best in shallow water depth (≦10cm) than in the greater flooding depths. Saturated soils with no standing water at the early stage of reed growth increased reed survival and water depth can be increased as the reeds grow. During the process of water supplementation, soil salinity was reduced significantly. Soil salinity was reduced dramatically at early and middle stages of reed growth, but it increased slightly at the later stage. Soil pH increased greatly during the experiment. Soil total nitrogen (TN) and total organic carbon (TOC) showed contrasting changes, with soil TN decreasing and TOC increasing. To best manage reed wetlands restoration, we suggest saturating wetland in the spring to stimulate reed germination, increasing surface water depth up to 15cm at the stage of reed rapid growth, and then reducing water depth during the later growth stage.
[Mh] Termos MeSH primário: Recuperação e Remediação Ambiental/métodos
Rios
Zonas Úmidas
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Biomassa
Aves
Carbono/análise
China
Condutividade Elétrica
Ambiente Controlado
Concentração de Íons de Hidrogênio
Nitrogênio/análise
Plantas
Salinidade
Solo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 7440-44-0 (Carbon); N762921K75 (Nitrogen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177692


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[PMID]:28431422
[Au] Autor:Stedman JL; Yarmush JM; Joshi MC; Kamath S; Schianodicola J
[Ad] Endereço:From the Department of Anesthesiology, New York Methodist Hospital, Brooklyn, New York.
[Ti] Título:How Long Is Too Long? The Prespiked Intravenous Debate.
[So] Source:Anesth Analg;124(5):1564-1568, 2017 May.
[Is] ISSN:1526-7598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: As the number of operative cases increases, there is a greater emphasis on reducing inefficiency while maintaining patient safety. Recently, the issue of prespiking intravenous (IV) bags was raised. No study has assessed whether the risk of infection is related to the length of time a sterile (IV) fluid bag has been spiked. After completion of a pilot study revealed no microbial growth within 24 hours of an IV spike, a larger formal study was undertaken to determine whether there was an increased infection risk within 4 hours of spiking an intravenous fluid bag. METHODS: Five IV administration sets were spiked and hung in busy perioperative areas once a week for a 5-week period. Five samples were drawn from each IV set. Approximately 10 mL was collected per sample. All samples were inoculated in 2 separate growth media. If any growth was noted, the sample was marked as positive. RESULTS: A total of 125 samples were collected over a period of 5 weeks, yielding 250 specimens. No samples were excluded from the study. No growth was identified in any of the specimens. The 95% confidence interval was estimated to be 0, 0.063. DISCUSSION: There was no bacterial growth in prespiked normal saline IV bags in a perioperative environment. Thus, prespiking of normal saline IV bags in advance should pose no risk of infection to a patient if prepared within 4 hours.
[Mh] Termos MeSH primário: Infecções Bacterianas/prevenção & controle
Infecção Hospitalar/prevenção & controle
Contaminação de Medicamentos/prevenção & controle
Embalagem de Medicamentos
Hidratação/métodos
Cloreto de Sódio/administração & dosagem
[Mh] Termos MeSH secundário: Infecções Bacterianas/microbiologia
Infecção Hospitalar/microbiologia
Esquema de Medicação
Ambiente Controlado
Hidratação/efeitos adversos
Hidratação/instrumentação
Seres Humanos
Infusões Intravenosas
Salas Cirúrgicas
Projetos Piloto
Medição de Risco
Fatores de Risco
Cloreto de Sódio/efeitos adversos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE
[do] DOI:10.1213/ANE.0000000000001951


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[PMID]:28399169
[Au] Autor:Li YZ; Fan XW; Chen Q; Zhong H
[Ad] Endereço:State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, P. R. China.
[Ti] Título:A photoperiod-responsive protein compendium and conceptual proteome roadmap outline in maize grown in growth chambers with controlled conditions.
[So] Source:PLoS One;12(4):e0174003, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Maize (Zea mays L.) is one of the major staple food crops of the world. However, high photoperiod sensitivity, especially for tropical germplasms, impedes attempts to improve maize agronomical traits by integration of tropical and temperate maize germplasms. Physiological and phenotypic responses of maize to photoperiod have widely been investigated based on multi-site field observations; however, proteome-based responsive mechanisms under controlled photoperiod regimes, nutrient and moisture soils are not yet well understood. In the present study, we sequenced and analyzed six proteomes of tropically-adapted and photoperiod-sensitive M9 inbred line at the vegetative 3 stage and proteomes from tropically-adapted and photoperiod-sensitive Shuang M9 (SM9) inbred line at the vegetative-tasseling stage. All plants were grown in growth chambers with controlled soil and temperature and three photoperiod regimes, a short photoperiod (SP) of 10 h light/14 h dark, a control neutral photoperiod (NP) of 12 h light/12 h dark, and a long photoperiod (LP) of 16 h light/8 h dark for a daily cycle. We identified 4,395 proteins of which 401 and 425 differentially-expressed proteins (DPs) were found in abundance in M9 leaves and in SM9 leaves as per SP/LP vs. NP, respectively. Some DPs showed responses to both SP and LP while some only responded to either SP or LP, depending on M9 or SM9. Our study showed that the photoperiodic response pathway, circadian clock rhythm, and high light density/intensity crosstalk with each other, but apparently differ from dark signaling routes. Photoperiod response involves light-responsive or dark-responsive proteins or both. The DPs positioned on the signaling routes from photoperiod changes to RNA/DNA responses involve the mago nashi homolog and glycine-rich RNA-binding proteins. Moreover, the cell-to-cell movement of ZCN14 through plasmodesmata is likely blocked under a 16-h-light LP. Here, we propose a photoperiodic model based on our findings and those from previous studies.
[Mh] Termos MeSH primário: Ambiente Controlado
Modelos Biológicos
Fotoperíodo
Proteoma
Zea mays/crescimento & desenvolvimento
Zea mays/metabolismo
[Mh] Termos MeSH secundário: Cromatografia
Cromatografia Líquida de Alta Pressão
Ritmo Circadiano/fisiologia
Regulação da Expressão Gênica/fisiologia
Ontologia Genética
Inflorescência/crescimento & desenvolvimento
Inflorescência/metabolismo
Folhas de Planta/crescimento & desenvolvimento
Folhas de Planta/metabolismo
Proteínas de Plantas/metabolismo
Plasmodesmos/metabolismo
Proteínas de Ligação a RNA/metabolismo
Especificidade da Espécie
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Proteome); 0 (RNA-Binding Proteins); 0 (glycine-rich RNA-binding protein, plant)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174003


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[PMID]:28231617
[Au] Autor:Ribic U; Klancnik A; Jersek B
[Ad] Endereço:Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
[Ti] Título:Characterization of Staphylococcus epidermidis strains isolated from industrial cleanrooms under regular routine disinfection.
[So] Source:J Appl Microbiol;122(5):1186-1196, 2017 May.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: The purpose of this study was the genotypic and phenotypic characterization of 57 strains of Staphylococcus epidermidis isolated from cleanroom environments, based on their biofilm formation and antimicrobial resistance profiles. METHODS AND RESULTS: Biofilm formation was investigated using real-time PCR (icaA, aap, bhp genes), the Congo red agar method and the crystal violet assay. The majority of the strains (59·7%; 34/57) did not form biofilms according to the crystal violet assay, although the biofilm-associated genes were present in 94·7% (54/57) of the strains. Of the biofilm formers (40·4%; 23/57), 39·1% (9/23) have been identified as strong biofilm formers (>4× crystal violet absorbance cut-off). Resistance to a commercial disinfectant and its quaternary ammonium active component, didecyl-dimethyl-ammonium chloride (DDAC), was determined according to minimum inhibitory concentrations (MICs) and the presence of the qac (quaternary ammonium compound) genes. More than 95% (55/57) of the Staph. epidermidis strains had the qacA/B and qacC genes, but not the other qac genes. The MICs for the disinfectant and DDAC varied among the Staph. epidermidis strains, although none were resistant. CONCLUSIONS: Although 59·6% of the Staph. epidermidis strains did not form biofilms and none were resistant to DDAC, more than 94% had the genetic basis for development of resistance to quaternary ammonium compounds, and among them at least 14·0% (8/57) might represent a high risk to cleanroom hygiene as strong biofim formers with qacA/B and qacC genes. SIGNIFICANCE AND IMPACT OF THE STUDY: To assure controlled cleanroom environments, bacterial strains isolated from cleanroom environments need to be characterized regularly using several investigative methods.
[Mh] Termos MeSH primário: Desinfetantes/farmacologia
Staphylococcus epidermidis/isolamento & purificação
[Mh] Termos MeSH secundário: Biofilmes/efeitos dos fármacos
Desinfecção
Ambiente Controlado
Seres Humanos
Indústrias/instrumentação
Indústrias/normas
Testes de Sensibilidade Microbiana
Compostos de Amônio Quaternário/farmacologia
Staphylococcus epidermidis/classificação
Staphylococcus epidermidis/efeitos dos fármacos
Staphylococcus epidermidis/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disinfectants); 0 (Quaternary Ammonium Compounds)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170224
[St] Status:MEDLINE
[do] DOI:10.1111/jam.13424



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