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[PMID]: | 29458459 |
[Au] Autor: | Klotz F; Brinkhoff T; Freese HM; Wietz M; Teske A; Simon M; Giebel HA |
[Ad] Endereço: | 1​Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, D-26111 Oldenburg, Germany. |
[Ti] Título: | Tritonibacter horizontis gen. nov., sp. nov., a member of the Rhodobacteraceae, isolated from the Deepwater Horizon oil spill. |
[So] Source: | Int J Syst Evol Microbiol;68(3):736-744, 2018 Mar. | [Is] ISSN: | 1466-5034 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | A heterotrophic, Gram-stain-negative, aerobic, sodium-requiring and motile bacterium was isolated from oil-contaminated surface water of the Gulf of Mexico during the Deepwater Horizon oil spill. Strain O3.65 showed highest 16S rRNA gene sequence similarity to Phaeobacter gallaeciensis BS107 and Phaeobacter inhibens T5 , both with 98.3 %, respectively. Based on complete genome analysis, highest similarity was observed to species of the genus Ruegeria. Strain O3.65 exhibited a broad salinity, temperature and pH range of 0.5-10 % NaCl, 4-45 °C and 5.5-9.0, respectively. The DNA G+C content of strain O3.65 was 61.5 mol%. The major respiratory lipoquinone was ubiquinone-10 (Q-10), the most dominant fatty acids (>1 %) comprised 18 : 1ω7c and 18 : 1ω7c 11-methyl, 10 : 0 3OH, 12 : 1 3OH, 14 : 1 3OH/3-oxo-14 : 0, 16 : 0, 16 : 0 2OH, 18 : 1 2OH and 12 : 1. The polar lipid pattern indicated presence of phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid, two unidentified phospholipids and seven unidentified lipids. On Difco marine broth agar, strain O3.65 formed smooth, shiny white to beige and convex colonies with regular edges. Phylogenetic, phylogenomic and phenotypic differences revealed that strain O3.65 represents a new species of a novel genus within the family Rhodobacteraceae, for which we propose the name Tritonibacter horizontis gen. nov., sp. nov. The type strain of the type species is O3.65 (=DSM 101689 =LMG 29740 ). |
[Mh] Termos MeSH primário: |
Poluição por Petróleo Filogenia Rhodobacteraceae/classificação Água do Mar/microbiologia
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[Mh] Termos MeSH secundário: |
Técnicas de Tipagem Bacteriana Composição de Bases DNA Bacteriano/genética Ácidos Graxos/química Golfo do México Processos Heterotróficos Fosfolipídeos/química RNA Ribossômico 16S/genética Rhodobacteraceae/genética Rhodobacteraceae/isolamento & purificação Análise de Sequência de DNA Ubiquinona/química Poluentes Químicos da Água
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 0 (Water Pollutants, Chemical); 1339-63-5 (Ubiquinone); I7T5V2W47R (Ubiquinone Q2) |
[Em] Mês de entrada: | 1803 |
[Cu] Atualização por classe: | 180308 |
[Lr] Data última revisão:
| 180308 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 180221 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1099/ijsem.0.002573 |
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