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[PMID]:29239718
[Au] Autor:Ordeig L; Garcia-Cehic D; Gregori J; Soria ME; Nieto-Aponte L; Perales C; Llorens M; Chen Q; Riveiro-Barciela M; Buti M; Esteban R; Esteban JI; Rodriguez-Frias F; Quer J
[Ad] Endereço:1​Liver Unit, Liver Disease Laboratory-Viral Hepatitis, Internal Medicine Department, Vall d'Hebron Institut Recerca (VHIR)-Hospital Universitari Vall d'Hebron (HUVH), 08035, Barcelona, Spain.
[Ti] Título:New hepatitis C virus genotype 1 subtype naturally harbouring resistance-associated mutations to NS5A inhibitors.
[So] Source:J Gen Virol;99(1):97-102, 2018 Jan.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hepatitis C virus (HCV) is a highly divergent virus currently classified into seven major genotypes and 86 subtypes (ICTV, June 2017), which can have differing responses to therapy. Accurate genotyping/subtyping using high-resolution HCV subtyping enables confident subtype identification, identifies mixed infections and allows detection of new subtypes. During routine genotyping/subtyping, one sample from an Equatorial Guinea patient could not be classified into any of the subtypes. The complete genomic sequence was compared to reference sequences by phylogenetic and sliding window analysis. Resistance-associated substitutions (RASs) were assessed by deep sequencing. The unclassified HCV genome did not belong to any of the existing genotype 1 (G1) subtypes. Sliding window analysis along the complete genome ruled out recombination phenomena suggesting that it belongs to a new HCV G1 subtype. Two NS5A RASs (L31V+Y93H) were found to be naturally combined in the genome which could limit treatment possibilities in patients infected with this subtype.
[Mh] Termos MeSH primário: Farmacorresistência Viral/genética
Genótipo
Hepacivirus/genética
Mutação
Filogenia
Proteínas não Estruturais Virais/genética
[Mh] Termos MeSH secundário: Anilidas/uso terapêutico
Antivirais/uso terapêutico
Benzimidazóis/uso terapêutico
Carbamatos/uso terapêutico
Guiné Equatorial
Feminino
Fluorenos/uso terapêutico
Expressão Gênica
Hepacivirus/classificação
Hepacivirus/efeitos dos fármacos
Hepatite C/tratamento farmacológico
Hepatite C/patologia
Hepatite C/virologia
Seres Humanos
Imidazóis/uso terapêutico
Testes de Sensibilidade Microbiana
Meia-Idade
Análise de Sequência de DNA
Sulfonamidas/uso terapêutico
Uracila/análogos & derivados
Uracila/uso terapêutico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABT-267); 0 (ABT-333); 0 (Anilides); 0 (Antiviral Agents); 0 (BMS-790052); 0 (Benzimidazoles); 0 (Carbamates); 0 (Fluorenes); 0 (Imidazoles); 0 (NS-5 protein, hepatitis C virus); 0 (Sulfonamides); 0 (Viral Nonstructural Proteins); 013TE6E4WV (ledipasvir); 56HH86ZVCT (Uracil)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000996


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[PMID]:28748565
[Au] Autor:Portik DM; Leaché AD; Rivera D; Barej MF; Burger M; Hirschfeld M; Rödel MO; Blackburn DC; Fujita MK
[Ad] Endereço:Department of Biology, The University of Texas at Arlington, Arlington, TX, USA.
[Ti] Título:Evaluating mechanisms of diversification in a Guineo-Congolian tropical forest frog using demographic model selection.
[So] Source:Mol Ecol;26(19):5245-5263, 2017 Oct.
[Is] ISSN:1365-294X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The accumulation of biodiversity in tropical forests can occur through multiple allopatric and parapatric models of diversification, including forest refugia, riverine barriers and ecological gradients. Considerable debate surrounds the major diversification process, particularly in the West African Lower Guinea forests, which contain a complex geographic arrangement of topographic features and historical refugia. We used genomic data to investigate alternative mechanisms of diversification in the Gaboon forest frog, Scotobleps gabonicus, by first identifying population structure and then performing demographic model selection and spatially explicit analyses. We found that a majority of population divergences are best explained by allopatric models consistent with the forest refugia hypothesis and involve divergence in isolation with subsequent expansion and gene flow. These population divergences occurred simultaneously and conform to predictions based on climatically stable regions inferred through ecological niche modelling. Although forest refugia played a prominent role in the intraspecific diversification of S. gabonicus, we also find evidence for potential interactions between landscape features and historical refugia, including major rivers and elevational barriers such as the Cameroonian Volcanic Line. We outline the advantages of using genomewide variation in a model-testing framework to distinguish between alternative allopatric hypotheses, and the pitfalls of limited geographic and molecular sampling. Although phylogeographic patterns are often species-specific and related to life-history traits, additional comparative studies incorporating genomic data are necessary for separating shared historical processes from idiosyncratic responses to environmental, climatic and geological influences on diversification.
[Mh] Termos MeSH primário: Anuros/classificação
Biodiversidade
Evolução Biológica
Filogenia
[Mh] Termos MeSH secundário: Animais
Camarões
Congo
DNA Mitocondrial/genética
Guiné Equatorial
Florestas
Gabão
Fluxo Gênico
Modelos Biológicos
Nigéria
Filogeografia
Clima Tropical
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1111/mec.14266


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[PMID]:28622465
[Ti] Título:Validation of maternal and neonatal tetanus elimination in Equatorial Guinea, 2016.
[Ti] Título:alidation de l'élimination du tétanos maternel et néonatal en Guinée équatoriale, 2016..
[So] Source:Wkly Epidemiol Rec;92(24):333-44, 2017 06 16.
[Is] ISSN:0049-8114
[Cp] País de publicação:Switzerland
[La] Idioma:eng; fre
[Mh] Termos MeSH primário: Erradicação de Doenças/métodos
Programas de Imunização/organização & administração
Áreas de Pobreza
Tétano/prevenção & controle
[Mh] Termos MeSH secundário: Adulto
Análise por Conglomerados
Guiné Equatorial
Feminino
Saúde Global
Guias como Assunto
Seres Humanos
Lactente
Mortalidade Infantil
Recém-Nascido
Mortalidade Materna
Inquéritos e Questionários
Tétano/mortalidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170618
[St] Status:MEDLINE


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[PMID]:28403879
[Au] Autor:Gómez-Barroso D; García-Carrasco E; Herrador Z; Ncogo P; Romay-Barja M; Ondo Mangue ME; Nseng G; Riloha M; Santana MA; Valladares B; Aparicio P; Benito A
[Ad] Endereço:CIBERESP, National Centre of Epidemiology, Carlos III Institute of Health (ISCIII), Madrid, Spain. dgomez@externos.isciii.es.
[Ti] Título:Spatial clustering and risk factors of malaria infections in Bata district, Equatorial Guinea.
[So] Source:Malar J;16(1):146, 2017 Apr 12.
[Is] ISSN:1475-2875
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The transmission of malaria is intense in the majority of the countries of sub-Saharan Africa, particularly in those that are located along the Equatorial strip. The present study aimed to describe the current distribution of malaria prevalence among children and its environment-related factors as well as to detect malaria spatial clusters in the district of Bata, in Equatorial Guinea. METHODS: From June to August 2013 a representative cross-sectional survey using a multistage, stratified, cluster-selected sample was carried out of children in urban and rural areas of Bata District. All children were tested for malaria using rapid diagnostic tests (RDTs). Results were linked to each household by global position system data. Two cluster analysis methods were used: hot spot analysis using the Getis-Ord Gi statistic, and the SaTScan™ spatial statistic estimates, based on the assumption of a Poisson distribution to detect spatial clusters. In addition, univariate associations and Poisson regression model were used to explore the association between malaria prevalence at household level with different environmental factors. RESULTS: A total of 1416 children aged 2 months to 15 years living in 417 households were included in this study. Malaria prevalence by RDTs was 47.53%, being highest in the age group 6-15 years (63.24%, p < 0.001). Those children living in rural areas were there malaria risk is greater (65.81%) (p < 0.001). Malaria prevalence was higher in those houses located <1 km from a river and <3 km to a forest (IRR: 1.31; 95% CI 1.13-1.51 and IRR: 1.44; 95% CI 1.25-1.66, respectively). Poisson regression analysis also showed a decrease in malaria prevalence with altitude (IRR: 0.73; 95% CI 0.62-0.86). A significant cluster inland of the district, in rural areas has been found. CONCLUSIONS: This study reveals a high prevalence of RDT-based malaria among children in Bata district. Those households situated in inland rural areas, near to a river, a green area and/or at low altitude were a risk factor for malaria. Spatial tools can help policy makers to promote new recommendations for malaria control.
[Mh] Termos MeSH primário: Análise por Conglomerados
Malária/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Animais
Criança
Pré-Escolar
Estudos Transversais
Meio Ambiente
Guiné Equatorial/epidemiologia
Feminino
Seres Humanos
Imunocromatografia
Lactente
Masculino
Prevalência
Fatores de Risco
População Rural
Análise Espacial
Topografia Médica
População Urbana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1186/s12936-017-1794-z


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[PMID]:28367743
[Au] Autor:Zheng X; Lin M; Xie DD; Li J; Chen JT; Eyi UM; Monte-Nguba SM; Ehapo JC; Yang H; Yang HT; Yang LY
[Ad] Endereço:a Central Laboratory , Chaozhou Central Hospital Affiliated to Southern Medical University , Chaozhou , Guangdong , People's Republic of China.
[Ti] Título:Prevalence of HIV and malaria: a cross-sectional study on Bioko Island, Equatorial Guinea.
[So] Source:Afr J AIDS Res;16(1):65-70, 2017 Mar.
[Is] ISSN:1727-9445
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:Malaria and HIV are two of the most severe public health problems in Africa. However, epidemiological data on Bioko Island is scarce. To investigate the prevalence of malaria and HIV infections and assess association of malaria and HIV infections and possible confounding factors, we performed a cross-sectional survey of people of malaria-endemic Bioko Island, Equatorial Guinea. A cross-sectional study of 1 526 subjects was carried out to determine the prevalence of malaria and HIV infection in Malabo region hospital on Bioko Island. Questionnaires were administered and venous blood samples were drawn for malaria parasites and HIV detection. The prevalence of participants infected with malaria and HIV in this area were 13.8% and 6.6% respectively. The average prevalence of co-infection for malaria and HIV was 0.92%. HIV-infection was significantly associated with the age and gender. Malaria infections were significantly associated with the age. This study showed that the prevalence of HIV and malaria on Bioko Island was higher than expected, although the co-infection prevalence of malaria and HIV was low. The results also indicated that malaria and HIV infections lead to more public health risk to youngsters and women.
[Mh] Termos MeSH primário: Infecções por HIV/epidemiologia
Malária/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Criança
Pré-Escolar
Coinfecção
Estudos Transversais
Guiné Equatorial/epidemiologia
Feminino
Seres Humanos
Lactente
Ilhas
Malária/parasitologia
Masculino
Meia-Idade
Razão de Chances
Carga Parasitária
Vigilância da População
Prevalência
Fatores de Risco
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170418
[Lr] Data última revisão:
170418
[Sb] Subgrupo de revista:IM; X
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.2989/16085906.2016.1257495


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[PMID]:28225668
[Au] Autor:Lu F; Culleton R; Zhang M; Ramaprasad A; von Seidlein L; Zhou H; Zhu G; Tang J; Liu Y; Wang W; Cao Y; Xu S; Gu Y; Li J; Zhang C; Gao Q; Menard D; Pain A; Yang H; Zhang Q; Cao J
[Ad] Endereço:Jiangsu Institute of Parasitic Diseases, Wuxi, China.
[Ti] Título:Emergence of Indigenous Artemisinin-Resistant Plasmodium falciparum in Africa.
[So] Source:N Engl J Med;376(10):991-3, 2017 03 09.
[Is] ISSN:1533-4406
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Antimaláricos/uso terapêutico
Artemisininas/uso terapêutico
Resistência a Medicamentos
Malária Falciparum/tratamento farmacológico
Plasmodium falciparum/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adulto
África
Ásia Sudeste
Quimioterapia Combinada
Guiné Equatorial
Seres Humanos
Malária Falciparum/transmissão
Masculino
Mutação
Papua Nova Guiné
Parasitemia
Plasmodium falciparum/genética
Plasmodium falciparum/isolamento & purificação
Polimorfismo de Nucleotídeo Único
Quinolinas/uso terapêutico
[Pt] Tipo de publicação:CASE REPORTS; LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antimalarials); 0 (Artemisinins); 0 (Quinolines); 6A9O50735X (dihydroartemisinin); A0HV2Q956Y (piperaquine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE
[do] DOI:10.1056/NEJMc1612765


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[PMID]:28219992
[Au] Autor:Wilson BK; Kaur H; Allan EL; Lozama A; Bell D
[Ad] Endereço:Intellectual Ventures Laboratory, Bellevue, Washington.
[Ti] Título:A New Handheld Device for the Detection of Falsified Medicines: Demonstration on Falsified Artemisinin-Based Therapies from the Field.
[So] Source:Am J Trop Med Hyg;96(5):1117-1123, 2017 May.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AbstractPoor-quality medicines are a major problem for health-care systems in resource-poor settings as identifying falsified medicines requires a complex laboratory infrastructure such as a Medicines Quality Control Laboratory. We report here an evaluation of a low-cost, handheld near-infrared spectrometer (NIRS) device by analyzing a library of artemisinin-based combination therapy (ACT) medicines to determine its usefulness as a drug-screening tool. The "SCiO" research prototype device was used to collect NIR spectra of a library of ACT and artesunate monotherapy medicine samples previously collected in Bioko Island and Equatorial Guinea and Kintampo, Ghana. The quality of these samples had been categorized as falsified, substandard, and quality assured based on the amount of stated active pharmaceutical ingredients detected using high-performance liquid chromatography photodiode array. Numerical analyses were performed on the NIR spectra to assess the usefulness of NIR to identify falsified and substandard medicines. The NIRS device was successful at detecting falsified medicines in all cases where the library contained both quality assured and falsified medicines of the same stated brand of medicines. The NIRS device was successful at identifying substandard amounts of artesunate but not amodiaquine in the ACT samples ( = 15) of artesunate-amodiaquine. This work reveals that this low-cost, portable NIRS device is promising for screening ACTs for falsified samples and could enable widespread drug screening at all points of the health system.
[Mh] Termos MeSH primário: Amodiaquina/análise
Antimaláricos/análise
Artemisininas/análise
Computadores de Mão
Medicamentos Falsificados/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Combinação de Medicamentos
Contaminação de Medicamentos/prevenção & controle
Guiné Equatorial
Gana
Seres Humanos
Aplicativos Móveis
Controle de Qualidade
Sensibilidade e Especificidade
Espectrofotometria Infravermelho/instrumentação
Espectrofotometria Infravermelho/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimalarials); 0 (Artemisinins); 0 (Counterfeit Drugs); 0 (Drug Combinations); 0 (amodiaquine, artesunate drug combination); 220236ED28 (Amodiaquine); 60W3249T9M (artesunate); 9RMU91N5K2 (artemisinine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0904


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[PMID]:28086777
[Au] Autor:Berzosa P; Esteban-Cantos A; García L; González V; Navarro M; Fernández T; Romay-Barja M; Herrador Z; Rubio JM; Ncogo P; Santana-Morales M; Valladares B; Riloha M; Benito A
[Ad] Endereço:Malaria Laboratory, National Centre of Tropical Medicine, Institute of Health Carlos III, C/Monforte de Lemos 5, 28029, Madrid, Spain. pberzosa@isciii.es.
[Ti] Título:Profile of molecular mutations in pfdhfr, pfdhps, pfmdr1, and pfcrt genes of Plasmodium falciparum related to resistance to different anti-malarial drugs in the Bata District (Equatorial Guinea).
[So] Source:Malar J;16(1):28, 2017 Jan 13.
[Is] ISSN:1475-2875
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The emergence of drug resistance in Plasmodium falciparum has been a major contributor to the global burden of malaria. Drug resistance complicates treatment, and it is one of the most important problems in malaria control. This study assessed the level of mutations in P. falciparum genes, pfdhfr, pfdhps, pfmdr1, and pfcrt, related to resistance to different anti-malarial drugs, in the Continental Region of Equatorial Guinea, after 8 years of implementing artesunate combination therapies as the first-line treatment. RESULTS: A triple mutant of pfdhfr (51I/59R/108N), which conferred resistance to sulfadoxine/pyrimethamine (SP), was found in 78% of samples from rural settings; its frequency was significantly different between urban and rural settings (p = 0.007). The 164L mutation was detected for the first time in this area, in rural settings (1.4%). We also identified three classes of previously described mutants and their frequencies: the partially resistant (pfdhfr 51I/59R/108N + pfdhps 437G), found at 54% (95% CI 47.75-60.25); the fully resistant (pfdhfr 51I/59R/108N + pfdhps 437G/540E), found at 28% (95% CI 7.07-14.93); and the super resistant (pfdhfr 51I/59R/108N + pfdhps 437G/540E/581G), found at 6% (95% CI 0.48-4.32). A double mutation in pfmdr1 (86Y + 1246Y) was detected at 2% (95% CI 0.24-3.76) frequency, distributed in both urban and rural samples. A combination of single mutations in the pfmdr1 and pfcrt genes (86Y + 76T), which was related to resistance to chloroquine and amodiaquine, was detected in 22% (95% CI 16.8-27.2) of samples from the area. CONCLUSIONS: The high level of mutations detected in P. falciparum genes related to SP resistance could be linked to the unsuccessful withdrawal of SP treatment in this area. Drug resistance can reduce the efficacy of intermittent prophylactic treatment with SP for children under 5 years old and for pregnant women. Although a high number of mutations was detected, the efficacy of the first-line treatment, artemisinin/amodiaquine, was not affected. To avoid increases in the numbers, occurrence, and spread of mutations, and to protect the population, the Ministry of Health should ensure that health centres and hospitals are supplied with appropriate first-line treatments for malaria.
[Mh] Termos MeSH primário: Antimaláricos/farmacologia
Di-Hidropteroato Sintase/genética
Resistência a Medicamentos
Proteínas de Membrana Transportadoras/genética
Proteínas Associadas à Resistência a Múltiplos Medicamentos/genética
Plasmodium falciparum/efeitos dos fármacos
Proteínas de Protozoários/genética
Tetra-Hidrofolato Desidrogenase/genética
[Mh] Termos MeSH secundário: Estudos Transversais
Combinação de Medicamentos
Guiné Equatorial
Frequência do Gene
Genótipo
Seres Humanos
Mutação
Plasmodium falciparum/enzimologia
Plasmodium falciparum/genética
Plasmodium falciparum/isolamento & purificação
Pirimetamina/farmacologia
Sulfadoxina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimalarials); 0 (Drug Combinations); 0 (Mdr1 protein, Plasmodium falciparum); 0 (Membrane Transport Proteins); 0 (Multidrug Resistance-Associated Proteins); 0 (PfCRT protein, Plasmodium falciparum); 0 (Protozoan Proteins); 37338-39-9 (fanasil, pyrimethamine drug combination); 88463U4SM5 (Sulfadoxine); EC 1.5.1.3 (Tetrahydrofolate Dehydrogenase); EC 2.5.1.15 (Dihydropteroate Synthase); Z3614QOX8W (Pyrimethamine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170115
[St] Status:MEDLINE
[do] DOI:10.1186/s12936-016-1672-0


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[PMID]:27795385
[Au] Autor:Guerra M; Neres R; Salgueiro P; Mendes C; Ndong-Mabale N; Berzosa P; de Sousa B; Arez AP
[Ad] Endereço:Global Health and Tropical Medicine (GHTM), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), Lisbon, Portugal.
[Ti] Título:Plasmodium falciparum Genetic Diversity in Continental Equatorial Guinea before and after Introduction of Artemisinin-Based Combination Therapy.
[So] Source:Antimicrob Agents Chemother;61(1), 2017 Jan.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Efforts to control malaria may affect malaria parasite genetic variability and drug resistance, the latter of which is associated with genetic events that promote mechanisms to escape drug action. The worldwide spread of drug resistance has been a major obstacle to controlling Plasmodium falciparum malaria, and thus the study of the origin and spread of associated mutations may provide some insights into the prevention of its emergence. This study reports an analysis of P. falciparum genetic diversity, focusing on antimalarial resistance-associated molecular markers in two socioeconomically different villages in mainland Equatorial Guinea. The present study took place 8 years after a previous one, allowing the analysis of results before and after the introduction of an artemisinin-based combination therapy (ACT), i.e., artesunate plus amodiaquine. Genetic diversity was assessed by analysis of the Pfmsp2 gene and neutral microsatellite loci. Pfdhps and Pfdhfr alleles associated with sulfadoxine-pyrimethamine (SP) resistance and flanking microsatellite loci were investigated, and the prevalences of drug resistance-associated point mutations of the Pfcrt, Pfmdr1, Pfdhfr, and Pfdhps genes were estimated. Further, to monitor the use of ACT, we provide the baseline prevalences of K13 propeller mutations and Pfmdr1 copy numbers. After 8 years, noticeable differences occurred in the distribution of genotypes conferring resistance to chloroquine and SP, and the spread of mutated genotypes differed according to the setting. Regarding artemisinin resistance, although mutations reported as being linked to artemisinin resistance were not present at the time, several single nucleotide polymorphisms (SNPs) were observed in the K13 gene, suggesting that closer monitoring should be maintained to prevent the possible spread of artemisinin resistance in Africa.
[Mh] Termos MeSH primário: Amodiaquina/uso terapêutico
Antimaláricos/uso terapêutico
Artemisininas/uso terapêutico
Resistência a Medicamentos/genética
Variação Genética
Malária Falciparum/tratamento farmacológico
Plasmodium falciparum/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antígenos de Protozoários/genética
Antígenos de Protozoários/metabolismo
Cloroquina/uso terapêutico
Variações do Número de Cópias de DNA
Combinação de Medicamentos
Guiné Equatorial
Feminino
Loci Gênicos
Genótipo
Seres Humanos
Malária Falciparum/parasitologia
Masculino
Proteínas de Membrana Transportadoras/genética
Proteínas de Membrana Transportadoras/metabolismo
Repetições de Microssatélites
Plasmodium falciparum/genética
Plasmodium falciparum/crescimento & desenvolvimento
Mutação Puntual
Proteínas de Protozoários/genética
Proteínas de Protozoários/metabolismo
Pirimetamina/uso terapêutico
Sulfadoxina/uso terapêutico
Tetra-Hidrofolato Desidrogenase/genética
Tetra-Hidrofolato Desidrogenase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Protozoan); 0 (Antimalarials); 0 (Artemisinins); 0 (Drug Combinations); 0 (Membrane Transport Proteins); 0 (PfCRT protein, Plasmodium falciparum); 0 (Protozoan Proteins); 0 (merozoite surface protein 2, Plasmodium); 220236ED28 (Amodiaquine); 37338-39-9 (fanasil, pyrimethamine drug combination); 60W3249T9M (artesunate); 88463U4SM5 (Sulfadoxine); 886U3H6UFF (Chloroquine); EC 1.5.1.3 (Tetrahydrofolate Dehydrogenase); Z3614QOX8W (Pyrimethamine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161101
[St] Status:MEDLINE


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[PMID]:27746398
[Au] Autor:Pérez-Lago L; Izco S; Herranz M; Tudó G; Carcelén M; Comas I; Sierra O; González-Martín J; Ruiz-Serrano MJ; Eyene J; Bouza E; García de Viedma D
[Ad] Endereço:Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades respiratorias, CIBERES, Spain.
[Ti] Título:A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial Guinea 15 years after its emergence.
[So] Source:Clin Microbiol Infect;23(2):92-97, 2017 Feb.
[Is] ISSN:1469-0691
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Molecular epidemiology techniques in tuberculosis (TB) can identify high-risk strains that are actively transmitted. We aimed to implement a novel strategy to optimize the identification and control of multidrug-resistant (MDR) TB in a specific population. METHODS: We developed a strain-specific PCR tailored from whole genome sequencing (WGS) data to track a specific MDR prevalent strain in Equatorial Guinea (EG-MDR). RESULTS: The PCR was applied prospectively on remnants of GeneXpert reaction mixtures owing to the lack of culture facilities in Equatorial Guinea. In 147 (93%) of 158 cases, we were able to differentiate between infection by the EG-MDR strain or by any other strain and found that 44% of all rifampicin-resistant TB cases were infected by EG-MDR. We also analysed 93 isolates obtained from Equatorial Guinea 15 years ago, before MDR-TB had become the problem it is today. We found that two of the scarce historical MDR cases were infected by EG-MDR. WGS revealed low variability-six single nucleotide polymorphisms acquired by this strain over 15 years-likely because of the lack in the country of a specific program to treat MDR-TB. CONCLUSIONS: Our novel strategy, which integrated WGS analysis and strain-specific PCRs, represents a low-cost, rapid and transferable strategy that allowed a prospective efficient survey and fast historical analysis of MDR-TB in a population.
[Mh] Termos MeSH primário: Genoma Bacteriano
Genômica
Mycobacterium tuberculosis/genética
Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia
Tuberculose Resistente a Múltiplos Medicamentos/microbiologia
[Mh] Termos MeSH secundário: Alelos
Antituberculosos/farmacologia
Farmacorresistência Bacteriana/efeitos dos fármacos
Guiné Equatorial/epidemiologia
Genômica/métodos
Seres Humanos
Testes de Sensibilidade Microbiana
Repetições Minissatélites
Tipagem de Sequências Multilocus
Mycobacterium tuberculosis/efeitos dos fármacos
Reação em Cadeia da Polimerase/métodos
Polimorfismo de Nucleotídeo Único
Prevalência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antitubercular Agents)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE



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