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  1 / 764 MEDLINE  
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[PMID]:28449693
[Au] Autor:Mariën J; Borremans B; Gryseels S; Soropogui B; De Bruyn L; Bongo GN; Becker-Ziaja B; de Bellocq JG; Günther S; Magassouba N; Leirs H; Fichet-Calvet E
[Ad] Endereço:Evolutionary Ecology Group, University of Antwerp, Antwerp, Belgium. Joachim.marien@uantwerpen.be.
[Ti] Título:No measurable adverse effects of Lassa, Morogoro and Gairo arenaviruses on their rodent reservoir host in natural conditions.
[So] Source:Parasit Vectors;10(1):210, 2017 Apr 27.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In order to optimize net transmission success, parasites are hypothesized to evolve towards causing minimal damage to their reservoir host while obtaining high shedding rates. For many parasite species however this paradigm has not been tested, and conflicting results have been found regarding the effect of arenaviruses on their rodent host species. The rodent Mastomys natalensis is the natural reservoir host of several arenaviruses, including Lassa virus that is known to cause Lassa haemorrhagic fever in humans. Here, we examined the effect of three arenaviruses (Gairo, Morogoro and Lassa virus) on four parameters of wild-caught Mastomys natalensis: body mass, head-body length, sexual maturity and fertility. After correcting for the effect of age, we compared these parameters between arenavirus-positive (arenavirus RNA or antibody) and negative animals using data from different field studies in Guinea (Lassa virus) and Tanzania (Morogoro and Gairo viruses). RESULTS: Although the sample sizes of our studies (1297, 749 and 259 animals respectively) were large enough to statistically detect small differences in body conditions, we did not observe any adverse effects of these viruses on Mastomys natalensis. We did find that sexual maturity was significantly positively related with Lassa virus antibody presence until a certain age, and with Gairo virus antibody presence in general. Gairo virus antibody-positive animals were also significantly heavier and larger than antibody-free animals. CONCLUSION: Together, these results suggest that the pathogenicity of arenaviruses is not severe in M. natalensis, which is likely to be an adaptation of these viruses to optimize transmission success. They also suggest that sexual behaviour might increase the probability of M. natalensis to become infected with arenaviruses.
[Mh] Termos MeSH primário: Infecções por Arenaviridae/veterinária
Arenavirus/isolamento & purificação
Portador Sadio/veterinária
Vetores de Doenças
Murinae/fisiologia
Murinae/virologia
[Mh] Termos MeSH secundário: Animais
Infecções por Arenaviridae/patologia
Infecções por Arenaviridae/virologia
Portador Sadio/patologia
Portador Sadio/virologia
Guiné
Tanzânia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2146-0


  2 / 764 MEDLINE  
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[PMID]:29253027
[Au] Autor:Panteli M; Benetos E; Dixon S
[Ad] Endereço:Centre for Digital Music, School of Electronic Engineering and Computer Science, Queen Mary University of London, London, United Kingdom.
[Ti] Título:A computational study on outliers in world music.
[So] Source:PLoS One;12(12):e0189399, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The comparative analysis of world music cultures has been the focus of several ethnomusicological studies in the last century. With the advances of Music Information Retrieval and the increased accessibility of sound archives, large-scale analysis of world music with computational tools is today feasible. We investigate music similarity in a corpus of 8200 recordings of folk and traditional music from 137 countries around the world. In particular, we aim to identify music recordings that are most distinct compared to the rest of our corpus. We refer to these recordings as 'outliers'. We use signal processing tools to extract music information from audio recordings, data mining to quantify similarity and detect outliers, and spatial statistics to account for geographical correlation. Our findings suggest that Botswana is the country with the most distinct recordings in the corpus and China is the country with the most distinct recordings when considering spatial correlation. Our analysis includes a comparison of musical attributes and styles that contribute to the 'uniqueness' of the music of each country.
[Mh] Termos MeSH primário: Música
Processamento de Sinais Assistido por Computador
[Mh] Termos MeSH secundário: Algoritmos
Benin
Botsuana
China
Análise por Conglomerados
Características Culturais
Geografia
Guiné
Seres Humanos
Linguagem
Modelos Estatísticos
Software
Sudão do Sul
Zimbábue
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189399


  3 / 764 MEDLINE  
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[PMID]:29190713
[Au] Autor:VanSteelandt A; Aho J; Franklin K; Likofata J; Kamgang JB; Keita S; Koivogui L; Magassouba N; Martel LD; Dahourou AG
[Ad] Endereço:Epidemiology, Informatics, Surveillance and Laboratory Branch, Department of Global Health Protection, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
[Ti] Título:Operational evaluation of rapid diagnostic testing for Ebola Virus Disease in Guinean laboratories.
[So] Source:PLoS One;12(11):e0188047, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Rapid Diagnostic Tests (RDTs) for Ebola Virus Disease (EVD) at the point of care have the potential to increase access and acceptability of EVD testing and the speed of patient isolation and secure burials for suspect cases. A pilot program for EVD RDTs in high risk areas of Guinea was introduced in October 2015. This paper presents concordance data between EVD RDTs and PCR testing in the field as well as an assessment of the acceptability, feasibility, and quality assurance of the RDT program. METHODS AND FINDINGS: Concordance data were compiled from laboratory surveillance databases. The operational measures of the laboratory-based EVD RDT program were evaluated at all 34 sentinel sites in Guinea through: (1) a technical questionnaire filled by the lab technicians who performed the RDTs, (2) a checklist filled by the evaluator during the site visits, and (3) direct observation of the lab technicians performing the quality control test. Acceptability of the EVD RDT was good for technicians, patients, and families although many technicians (69.8%) expressed concern for their safety while performing the test. The feasibility of the program was good based on average technician knowledge scores (6.6 out of 8) but basic infrastructure, equipment, and supplies were lacking. There was much room for improvement in quality assurance of the program. CONCLUSIONS: The implementation of new diagnostics in weak laboratory systems requires general training in quality assurance, biosafety and communication with patients in addition to specific training for the new test. Corresponding capacity building in terms of basic equipment and a long-term commitment to transfer supervision and quality improvement to national public health staff are necessary for successful implementation.
[Mh] Termos MeSH primário: Doença pelo Vírus Ebola/diagnóstico
Laboratórios
[Mh] Termos MeSH secundário: Algoritmos
Estudos de Viabilidade
Guiné/epidemiologia
Doença pelo Vírus Ebola/epidemiologia
Projetos Piloto
Sistemas Automatizados de Assistência Junto ao Leito
Garantia da Qualidade dos Cuidados de Saúde
Inquéritos e Questionários
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188047


  4 / 764 MEDLINE  
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[PMID]:29187944
[Au] Autor:Balde N; Camara A; Sobngwi-Tambekou J; Balti EV; Tchatchoua A; Fezeu L; Limen S; Ngamani S; Ngapout S; Kengne AP; Sobngwi E
[Ad] Endereço:University Hospital Donka, Conakry, Guinea.
[Ti] Título:Improving access to HbA1c in sub-Saharan Africa (IA3) cohort: cohort profile.
[So] Source:Pan Afr Med J;27:275, 2017.
[Is] ISSN:1937-8688
[Cp] País de publicação:Uganda
[La] Idioma:eng
[Ab] Resumo:Introduction: Glycated haemoglobin (HbA1c) is the best surrogate of average blood glucose control in diabetic patients, and lowering HbA1c significantly reduces diabetes complications. Moreover, immediate feedback of HbA1c measurement to patients may improve control. However, HbA1c is unavailable in most parts of Africa, a continent with one of the highest burden of diabetes. To translate these evidences, we are conducting a multicentric project in 10 health care facilities in Guinea and Cameroon to evaluate the feasibility and one-year benefit of affordable HbA1c measurement with immediate feedback to patients on diabetes control and related outcomes. Participants: We consecutively enrolled patients with diabetes mellitus independently of the type of disease. We hypothesised an average 1%-decrease in HbA1c in a 1000-patient study population, with a 20% increase in the number of patients reaching treatment goals within 12 months of intervention and follow-up. Findings to date: A total of 1, 349 diabetic patients aged 56.2±12.6 years are enrolled (813 in Cameroon and 536 in Guinea) of whom 59.8% are women. The mean duration of diabetes is 7.4±6.3 years and baseline HbA1c is 9.7±2.6% in Guinea and 8.6±2.5% in Cameroon. Future plans: To investigate whether the introduction of routine HbA1c measurement with immediate feedback to patients and provision of relevant education would improve diabetes control after one year. The impact of the intervention on diabetes associated-complications and mortality warrant further assessment in the long term.
[Mh] Termos MeSH primário: Glicemia/metabolismo
Diabetes Mellitus/terapia
Hemoglobina A Glicada/análise
Acesso aos Serviços de Saúde
[Mh] Termos MeSH secundário: Adulto
Idoso
Camarões
Estudos de Coortes
Estudos de Viabilidade
Feminino
Seguimentos
Guiné
Seres Humanos
Masculino
Meia-Idade
Educação de Pacientes como Assunto
Estudos Prospectivos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Glycated Hemoglobin A)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.11604/pamj.2017.27.275.10270


  5 / 764 MEDLINE  
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[PMID]:29049279
[Au] Autor:Jalloh MF; Robinson SJ; Corker J; Li W; Irwin K; Barry AM; Ntuba PN; Diallo AA; Jalloh MB; Nyuma J; Sellu M; VanSteelandt A; Ramsden M; Tracy L; Raghunathan PL; Redd JT; Martel L; Marston B; Bunnell R
[Ti] Título:Knowledge, Attitudes, and Practices Related to Ebola Virus Disease at the End of a National Epidemic - Guinea, August 2015.
[So] Source:MMWR Morb Mortal Wkly Rep;66(41):1109-1115, 2017 Oct 20.
[Is] ISSN:1545-861X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Health communication and social mobilization efforts to improve the public's knowledge, attitudes, and practices (KAP) regarding Ebola virus disease (Ebola) were important in controlling the 2014-2016 Ebola epidemic in Guinea (1), which resulted in 3,814 reported Ebola cases and 2,544 deaths.* Most Ebola cases in Guinea resulted from the washing and touching of persons and corpses infected with Ebola without adequate infection control precautions at home, at funerals, and in health facilities (2,3). As the 18-month epidemic waned in August 2015, Ebola KAP were assessed in a survey among residents of Guinea recruited through multistage cluster sampling procedures in the nation's eight administrative regions (Boké, Conakry, Faranah, Kankan, Kindia, Labé, Mamou, and Nzérékoré). Nearly all participants (92%) were aware of Ebola prevention measures, but 27% believed that Ebola could be transmitted by ambient air, and 49% believed they could protect themselves from Ebola by avoiding mosquito bites. Of the participants, 95% reported taking actions to avoid getting Ebola, especially more frequent handwashing (93%). Nearly all participants (91%) indicated they would send relatives with suspected Ebola to Ebola treatment centers, and 89% said they would engage special Ebola burial teams to remove corpses with suspected Ebola from homes. Of the participants, 66% said they would prefer to observe an Ebola-affected corpse from a safe distance at burials rather than practice traditional funeral rites involving corpse contact. The findings were used to guide the ongoing epidemic response and recovery efforts, including health communication, social mobilization, and planning, to prevent and respond to future outbreaks or sporadic cases of Ebola.
[Mh] Termos MeSH primário: Epidemias
Conhecimentos, Atitudes e Prática em Saúde
Doença pelo Vírus Ebola/epidemiologia
Doença pelo Vírus Ebola/psicologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Feminino
Guiné/epidemiologia
Seres Humanos
Masculino
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171020
[St] Status:MEDLINE
[do] DOI:10.15585/mmwr.mm6641a4


  6 / 764 MEDLINE  
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[PMID]:28827791
[Au] Autor:Kaboré JW; Ilboudo H; Noyes H; Camara O; Kaboré J; Camara M; Koffi M; Lejon V; Jamonneau V; MacLeod A; Hertz-Fowler C; Belem AMG; Matovu E; Bucheton B; Sidibe I; TrypanoGEN Research Group as members of The H3Africa Consortium
[Ad] Endereço:Centre International de Recherche-Développement sur l'Elevage en zone Subhumide (CIRDES), Bobo-Dioulasso, Burkina Faso.
[Ti] Título:Candidate gene polymorphisms study between human African trypanosomiasis clinical phenotypes in Guinea.
[So] Source:PLoS Negl Trop Dis;11(8):e0005833, 2017 Aug.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Human African trypanosomiasis (HAT), a lethal disease induced by Trypanosoma brucei gambiense, has a range of clinical outcomes in its human host in West Africa: an acute form progressing rapidly to second stage, spontaneous self-cure and individuals able to regulate parasitaemia at very low levels, have all been reported from endemic foci. In order to test if this clinical diversity is influenced by host genetic determinants, the association between candidate gene polymorphisms and HAT outcome was investigated in populations from HAT active foci in Guinea. METHODOLOGY AND RESULTS: Samples were collected from 425 individuals; comprising of 232 HAT cases, 79 subjects with long lasting positive and specific serology but negative parasitology and 114 endemic controls. Genotypes of 28 SNPs in eight genes passed quality control and were used for an association analysis. IL6 rs1818879 allele A (p = 0.0001, OR = 0.39, CI95 = [0.24-0.63], BONF = 0.0034) was associated with a lower risk of progressing from latent infection to active disease. MIF rs36086171 allele G seemed to be associated with an increased risk (p = 0.0239, OR = 1.65, CI95 = [1.07-2.53], BONF = 0.6697) but did not remain significant after Bonferroni correction. Similarly MIF rs12483859 C allele seems be associated with latent infections (p = 0.0077, OR = 1.86, CI95 = [1.18-2.95], BONF = 0.2157). We confirmed earlier observations that APOL1 G2 allele (DEL) (p = 0.0011, OR = 2.70, CI95 = [1.49-4.91], BONF = 0.0301) is associated with a higher risk and APOL1 G1 polymorphism (p = 0.0005, OR = 0.45, CI95 = [0.29-0.70], BONF = 0.0129) with a lower risk of developing HAT. No associations were found with other candidate genes. CONCLUSION: Our data show that host genes are involved in modulating Trypanosoma brucei gambiense infection outcome in infected individuals from Guinea with IL6 rs1818879 being associated with a lower risk of progressing to active HAT. These results enhance our understanding of host-parasite interactions and, ultimately, may lead to the development of new control tools.
[Mh] Termos MeSH primário: Predisposição Genética para Doença
Polimorfismo de Nucleotídeo Único
Tripanossomíase Africana/genética
Tripanossomíase Africana/patologia
[Mh] Termos MeSH secundário: Estudos de Associação Genética
Guiné
Seres Humanos
Fenótipo
Trypanosoma brucei gambiense
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005833


  7 / 764 MEDLINE  
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[PMID]:28721176
[Au] Autor:Merrill RD; Ward SE; Oppert MC; Schneider DA
[Ad] Endereço:International Border Team, Division of Global Migration and Quarantine, U.S. Centers for Disease Control and Prevention (CDC), Atlanta, USA.
[Ti] Título:From one to the other: responding to Ebola cases on either side of the line.
[So] Source:Pan Afr Med J;27(Suppl 1):12, 2017.
[Is] ISSN:1937-8688
[Cp] País de publicação:Uganda
[La] Idioma:eng
[Ab] Resumo:This case study is adapted from events that occurred along the Sierra Leone and Guinea land border during the 2014-2016 Ebola epidemic in West Africa. The response activities involved Sierra Leone and Guinea officials, along with assistance from U.S. Centers for Disease Control and Prevention (CDC) and the World Health Organisation (WHO). This case study builds upon an understanding of basic surveillance systems and outbreak response activities. Through this exercise, students will understand how to incorporate communication and coordination into surveillance and response efforts with counterparts across the border in neighbouring countries. This integration is important to reduce the spread of communicable diseases between neighbouring countries. The time required to complete this case study is 2-3 hours.
[Mh] Termos MeSH primário: Surtos de Doenças/prevenção & controle
Epidemias/prevenção & controle
Epidemiologia/educação
Doença pelo Vírus Ebola/epidemiologia
[Mh] Termos MeSH secundário: Centers for Disease Control and Prevention (U.S.)
Comunicação
Métodos Epidemiológicos
Guiné/epidemiologia
Seres Humanos
Cooperação Internacional
Serra Leoa/epidemiologia
Estados Unidos
Organização Mundial da Saúde
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170720
[St] Status:MEDLINE
[do] DOI:10.11604/pamj.supp.2017.27.1.12568


  8 / 764 MEDLINE  
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[PMID]:28665950
[Au] Autor:Ingelbeen B; Bah EI; Decroo T; Balde I; Nordenstedt H; van Griensven J; De Weggheleire A
[Ad] Endereço:Médecins Sans Frontières, Operational Centre Brussels, Conakry, Guinea.
[Ti] Título:Mortality among PCR negative admitted Ebola suspects during the 2014/15 outbreak in Conakry, Guinea: A retrospective cohort study.
[So] Source:PLoS One;12(6):e0180070, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Non-cases are suspect Ebola Virus Disease (EVD) cases testing negative by EVD RT-PCR after admission to an Ebola Treatment Centre (ETC). Admitting non-cases to an ETC prompts concerns on case- and workload in the ETC, risk for nosocomial EVD infection, and delays in diagnosis and disease-specific treatment. We retrospectively analysed characteristics, outcomes and determinants of death of EVD cases and non-cases admitted to the Conakry ETC in Guinea between 03/2014 and 09/2015. Of the 2362 admitted suspects who underwent full confirmatory PCR testing, 1540 (65.2%) were non-cases; among them 727 needed repeated confirmatory PCR testing resulting in 2.5 days (average) in the ETC isolation ward. Twenty-one patients tested positive on the repeat test, most in a period of flawed sampling for the initial test and none after introduction of PCR confirmation with geneXpert. No readmissions following nosocomial EVD infection were recorded. No combination of symptoms yielded acceptable sensitivity and specificity to allow differentiating confirmed from non-cases. Symptoms as ocular bleeding/redness have high specificity, but limited usefulness as not common. Admission delay and age distribution were not different for both groups. In total, 98 (20.6%) of 475 deaths in the ETC were non-cases. Most died within 24 hours after admission. Living in Conakry (aOR 1.78 (1.08-2.96)) was the strongest risk factor for death. Weeks with higher admission load had lower case fatality among non-cases, probably because more acute (and treatable) illnesses of contacts of known cases were admitted. These findings show high numbers of potentially critically ill non-cases need to be considered when setting up triage and referral of EVD suspect cases. Symptoms and risk factors alone do not allow differentiating the non-cases. Integration of highly-sensitive EVD diagnostic methods with short turnaround time in the triage of peripheral hospitals and dropping the systematic 2nd PCR for symptomatic early presenters could limit delays in access to adapted care of cases and seriously ill non-cases. Whether feasible without compromising outbreak control, and under which conditions, should be further assessed.
[Mh] Termos MeSH primário: Surtos de Doenças
Doença pelo Vírus Ebola/mortalidade
[Mh] Termos MeSH secundário: Adulto
Guiné/epidemiologia
Doença pelo Vírus Ebola/diagnóstico
Doença pelo Vírus Ebola/epidemiologia
Doença pelo Vírus Ebola/fisiopatologia
Seres Humanos
Reação em Cadeia da Polimerase
Estudos Retrospectivos
Fatores de Risco
Triagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180070


  9 / 764 MEDLINE  
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[PMID]:28640812
[Au] Autor:Carazo Perez S; Folkesson E; Anglaret X; Beavogui AH; Berbain E; Camara AM; Depoortere E; Lefevre A; Maes P; Malme KN; Malvy JD; Ombelet S; Poelaert G; Sissoko D; Tounkara A; Trbovic P; Piguet P; Antierens A
[Ad] Endereço:Médecins Sans Frontières, Brussels, Belgium.
[Ti] Título:Challenges in preparing and implementing a clinical trial at field level in an Ebola emergency: A case study in Guinea, West Africa.
[So] Source:PLoS Negl Trop Dis;11(6):e0005545, 2017 Jun.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During the large Ebola outbreak that affected West Africa in 2014 and 2015, studies were launched to evaluate potential treatments for the disease. A clinical trial to evaluate the effectiveness of the antiviral drug favipiravir was conducted in Guinea. This paper describes the main challenges of the implementation of the trial in the Ebola treatment center of Guéckédou. Following the principles of the Good Clinical Research Practices, we explored the aspects of the community's communication and engagement, ethical conduct, trial protocol compliance, informed consent of participants, ongoing benefit/risk assessment, record keeping, confidentiality of patients and study data, and roles and responsibilities of the actors involved. We concluded that several challenges have to be addressed to successfully implement a clinical trial during an international medical emergency but that the potential for collaboration between research teams and humanitarian organizations needs to be highlighted.
[Mh] Termos MeSH primário: Amidas/uso terapêutico
Antivirais/uso terapêutico
Ensaios Clínicos como Assunto/organização & administração
Doença pelo Vírus Ebola/tratamento farmacológico
Doença pelo Vírus Ebola/epidemiologia
Pirazinas/uso terapêutico
[Mh] Termos MeSH secundário: Ensaios Clínicos como Assunto/normas
Confidencialidade
Surtos de Doenças
Guiné/epidemiologia
Seres Humanos
Consentimento Livre e Esclarecido
Registros Médicos/normas
Projetos de Pesquisa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amides); 0 (Antiviral Agents); 0 (Pyrazines); EW5GL2X7E0 (favipiravir)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005545


  10 / 764 MEDLINE  
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[PMID]:28558022
[Au] Autor:Cimini E; Viola D; Cabeza-Cabrerizo M; Romanelli A; Tumino N; Sacchi A; Bordoni V; Casetti R; Turchi F; Martini F; Bore JA; Koundouno FR; Duraffour S; Michel J; Holm T; Zekeng EG; Cowley L; Garcia Dorival I; Doerrbecker J; Hetzelt N; Baum JHJ; Portmann J; Wölfel R; Gabriel M; Miranda O; Díaz G; Díaz JE; Fleites YA; Piñeiro CA; Castro CM; Koivogui L; Magassouba N; Diallo B; Ruibal P; Oestereich L; Wozniak DM; Lüdtke A; Becker-Ziaja B; Capobianchi MR; Ippolito G; Carroll MW; Günther S; Di Caro A; Muñoz-Fontela C; Agrati C
[Ad] Endereço:Department of Epidemiology and Pre-clinical research, National Institute for Infectious Diseases "Lazzaro Spallanzani", Rome, Italy.
[Ti] Título:Different features of Vδ2 T and NK cells in fatal and non-fatal human Ebola infections.
[So] Source:PLoS Negl Trop Dis;11(5):e0005645, 2017 May.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Human Ebola infection is characterized by a paralysis of the immune system. A signature of αß T cells in fatal Ebola infection has been recently proposed, while the involvement of innate immune cells in the protection/pathogenesis of Ebola infection is unknown. Aim of this study was to analyze γδ T and NK cells in patients from the Ebola outbreak of 2014-2015 occurred in West Africa, and to assess their association with the clinical outcome. METHODOLOGY/PRINCIPAL FINDINGS: Nineteen Ebola-infected patients were enrolled at the time of admission to the Ebola Treatment Centre in Guinea. Patients were divided in two groups on the basis of the clinical outcome. The analysis was performed by using multiparametric flow cytometry established by the European Mobile Laboratory in the field. A low frequency of Vδ2 T-cells was observed during Ebola infection, independently from the clinical outcome. Moreover, Vδ2 T-cells from Ebola patients massively expressed CD95 apoptotic marker, suggesting the involvement of apoptotic mechanisms in Vδ2 T-cell loss. Interestingly, Vδ2 T-cells from survivors expressed an effector phenotype and presented a lower expression of the CTLA-4 exhaustion marker than fatalities, suggesting a role of effector Vδ2 T-cells in the protection. Furthermore, patients with fatal Ebola infection were characterized by a lower NK cell frequency than patients with non fatal infection. In particular, both CD56bright and CD56dim NK frequency were very low both in fatal and non fatal infections, while a higher frequency of CD56neg NK cells was associated to non-fatal infections. Finally, NK activation and expression of NKp46 and CD158a were independent from clinical outcome. CONCLUSIONS/SIGNIFICANCES: Altogether, the data suggest that both effector Vδ2 T-cells and NK cells may play a role in the complex network of protective response to EBOV infection. Further studies are required to characterize the protective effector functions of Vδ2 and NK cells.
[Mh] Termos MeSH primário: Doença pelo Vírus Ebola/imunologia
Doença pelo Vírus Ebola/mortalidade
Células Matadoras Naturais/imunologia
Receptores de Antígenos de Linfócitos T gama-delta/imunologia
Subpopulações de Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Biomarcadores/metabolismo
Antígeno CD56/metabolismo
Antígeno CTLA-4/metabolismo
Bases de Dados Factuais
Ebolavirus
Feminino
Citometria de Fluxo
Guiné/epidemiologia
Seres Humanos
Ativação Linfocitária/imunologia
Masculino
Receptor 1 Desencadeador da Citotoxicidade Natural/metabolismo
Receptores KIR2DL1/metabolismo
Carga Viral
Receptor fas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (CD56 Antigen); 0 (CTLA-4 Antigen); 0 (FAS protein, human); 0 (NCR1 protein, human); 0 (Natural Cytotoxicity Triggering Receptor 1); 0 (Receptors, Antigen, T-Cell, gamma-delta); 0 (Receptors, KIR2DL1); 0 (fas Receptor)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005645



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