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[PMID]:29364989
[Au] Autor:Apolloni A; Nicolas G; Coste C; El Mamy AB; Yahya B; El Arbi AS; Gueya MB; Baba D; Gilbert M; Lancelot R
[Ad] Endereço:French Agricultural Research and International Cooperation Organization for Development (Cirad), Department of Biological Systems (Bios), UMR Animals, Health, Territories, Risks, and Ecosystems (Astre), Campus International de Baillarguet, 34398 Montpellier, France.
[Ti] Título:Towards the description of livestock mobility in Sahelian Africa: Some results from a survey in Mauritania.
[So] Source:PLoS One;13(1):e0191565, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Understanding spatio-temporal patterns of host mobility is a key factor to prevent and control animal and human diseases. This is utterly important in low-income countries, where animal disease epidemics have strong socio-economic impacts. In this article we analyzed a livestock mobility database, whose data have been collected by the Centre National d'Elevage et de Recherches Vétérinaires (CNERV) Mauritania, to describe its patterns and temporal evolution. Data were collected through phone and face-to-face interviews in almost all the regions in Mauritania over a period of roughly two weeks during June 2015. The analysis has shown the existence of two mobility patterns throughout the year: the first related to routine movements from January to August; the second strictly connected to the religious festivity of Tabaski that in 2014 occurred at the beginning of October. These mobility patterns are different in terms of animals involved (fewer cattle and dromedaries are traded around Tabaski), the means of transportation (the volume of animals moved by truck raises around Tabaski) and destinations (most of the animals are traded nationally around Tabaski). Due to the differences between these two periods, public health officers, researchers and other stakeholders should take account of the time of the year when implementing vaccination campaigns or creating surveillance networks.
[Mh] Termos MeSH primário: Gado
[Mh] Termos MeSH secundário: Animais
Mauritânia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191565


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[PMID]:29384308
[Au] Autor:Sy I; Traoré D; Niang Diène A; Koné B; Lô B; Faye O; Utzinger J; Cissé G; Tanner M
[Ti] Título:[Water, sanitation and diarrheal risk in Nouakchott Urban Community, Mauritania].
[Ti] Título:Eau potable, assainissement et risque de maladies diarrhéiques dans la Communauté Urbaine de Nouakchott, Mauritanie..
[So] Source:Sante Publique;29(5):741-750, 2017 Dec 05.
[Is] ISSN:0995-3914
[Cp] País de publicação:France
[La] Idioma:fre
[Ab] Resumo:INTRODUCTION: Drinking water and sanitation are two factors of inter-linked inextricably public health especially in the city of Nouakchott where the low availability of these services leads to a multitude of use and hygiene practices involving a complex socio-ecological system with an increased risk of waterborne diseases transmission (diarrhea, cholera, etc.). METHODS: Thus, this contribution analyzes the impact of socio-ecological system on the development of diarrheal diseases by using socio-environmental and epidemiological data from various sources (national surveys and registries consultation). RESULTS: Overall, the results show that only 25.6% of households have access to drinking water sources while 69.8% of the populations dispose improved latrines. Hence, the weakness in environmental sanitation conditions explains the level of diarrheal morbidity averring 12.8% at the urban level, with an unequal spatial distribution showing less affected communes such as Tevragh Zeina (9.1%) and municipalities more affected like Sebkha (19.1%). The distribution according to the age categories shows that children under 5 years are the most affected with 51.7% followed by people aged over 14 with 34.2%. The correlation analysis between socio-economic, environmental and epidemiological variables reveals a number of significant associations: untreated water consumption and diarrhea (R = 0.429); collection of wastewater and occurrence of diarrhea ; existence of improved latrine and reduction of diarrheal risk (R = 0.402). DISCUSSION: Therefore, exposure to diarrheal diseases through the prism of water and sanitation is a real public health problem that requires a systemic and integrated approach to improving environmental health.
[Mh] Termos MeSH primário: Diarreia/epidemiologia
Água Potável
Saneamento
[Mh] Termos MeSH secundário: Diarreia/prevenção & controle
Saúde Ambiental
Seres Humanos
Mauritânia/epidemiologia
Risco
Sanitários Públicos
População Urbana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drinking Water)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.3917/spub.175.0741


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[PMID]:28982126
[Au] Autor:Li H; Nykoluk M; Li L; Liu LR; Omange RW; Soule G; Schroeder LT; Toledo N; Kashem MA; Correia-Pinto JF; Liang B; Schultz-Darken N; Alonso MJ; Whitney JB; Plummer FA; Luo M
[Ad] Endereço:Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada.
[Ti] Título:Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques.
[So] Source:PLoS One;12(10):e0186079, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cynomolgus macaques are an increasingly important nonhuman primate model for HIV vaccine research. SIV-free animals without pre-existing anti-SIV immune responses are generally needed to evaluate the effect of vaccine-induced immune responses against the vaccine epitopes. Here, in order to select such animals for vaccine studies, we screened 108 naïve female Mauritian cynomolgus macaques for natural (baseline) antibodies to SIV antigens using a Bio-Plex multiplex system. The antigens included twelve 20mer peptides overlapping the twelve SIV protease cleavage sites (-10/+10), respectively (PCS peptides), and three non-PCS Gag or Env peptides. Natural antibodies to SIV antigens were detected in subsets of monkeys. The antibody reactivity to SIV was further confirmed by Western blot using purified recombinant SIV Gag and Env proteins. As expected, the immunization of monkeys with PCS antigens elicited anti-PCS antibodies. However, unexpectedly, antibodies to non-PCS peptides were also induced, as shown by both Bio-Plex and Western blot analyses, while the non-PCS peptides do not share sequence homology with PCS peptides. The presence of natural and vaccine cross-inducible SIV antibodies in Mauritian cynomolgus macaques should be considered in animal selection, experimental design and result interpretation, for their best use in HIV vaccine research.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Reações Cruzadas
Vacinas contra a SAIDS/imunologia
Vírus da Imunodeficiência Símia/imunologia
[Mh] Termos MeSH secundário: Animais
Fármacos Anti-HIV/uso terapêutico
Antígenos Virais/imunologia
Western Blotting
Vetores Genéticos
Macaca fascicularis
Mauritânia
Síndrome de Imunodeficiência Adquirida dos Símios/tratamento farmacológico
Síndrome de Imunodeficiência Adquirida dos Símios/imunologia
Síndrome de Imunodeficiência Adquirida dos Símios/prevenção & controle
Vírus da Imunodeficiência Símia/fisiologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-HIV Agents); 0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (SAIDS Vaccines)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186079


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[PMID]:28610374
[Au] Autor:Allen KE; N WPT; Welton LJ; Bauer AM
[Ad] Endereço:Department of Biology, Villanova University, 800 Lancaster Avenue, Villanova, Pennsylvania 19085, USA. Current address: Department of Ecology and Evolutionary Biology, University of Kansas, 1450 Jayhawk Boulevard, Lawrence, Kansas 66045, USA.. Kallen9@ku.edu.
[Ti] Título:A new species of Trachylepis (Squamata: Scincidae) from Central Africa and a key to the Trachylepis of West and Central Africa.
[So] Source:Zootaxa;4268(2):255-269, 2017 05 16.
[Is] ISSN:1175-5334
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:A new species of skink, Trachylepis gonwouoi sp. nov. is described from Cameroon and the Republic of the Congo. It differs from all other species of Trachylepis in Central-West Africa by a combination of number of keels on dorsal scales (3-5); moderate SVL (maximum size of 80 mm); number of scale rows at midbody (28-34); number of supracilliaries (6-10); a well defined lateral white stripe, bordered by black, extending from under the eye to the insertion of the hind limb; and a ventral color in life of bright blue-green. Trachylepis gonwouoi sp. nov. was found in association with disturbed forest at elevations from 50 to 1050m. This species is syntopic with T. affinis and T. maculilabris. In order to aid in the identification of Trachylepis in West and Central Africa with the addition of T. gonwouoi sp. nov., we provide an updated key to the Trachylepis found from Mauritania through the Democratic Republic of the Congo. This key combines previous literature that treated Western and Central African taxa separately and represents the most comprehensive key for Trachylepis in West-Central Africa to date.
[Mh] Termos MeSH primário: Lagartos
[Mh] Termos MeSH secundário: África Central
África Ocidental
Animais
Camarões
Congo
República Democrática do Congo
Mauritânia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.11646/zootaxa.4268.2.5


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[PMID]:28605299
[Au] Autor:Sas MA; Mertens M; Isselmou E; Reimer N; El Mamy BO; Doumbia B; Groschup MH
[Ad] Endereço:1 Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health , Greifswald - Isle of Riems, Germany .
[Ti] Título:Crimean-Congo Hemorrhagic Fever Virus-Specific Antibody Detection in Cattle in Mauritania.
[So] Source:Vector Borne Zoonotic Dis;17(8):582-587, 2017 Aug.
[Is] ISSN:1557-7759
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Crimean-Congo hemorrhagic fever virus (CCHFV) was detected for the first time in Mauritania in 1983 and several CCHFV outbreaks were reported in the following years. The last human case was diagnosed in 2015. However, no recent data exist about the prevalence of CCHFV in animals, although it is already described that prevalence studies in animals serve as good risk indicators. CCHFV can cause a severe hemorrhagic fever with a high case fatality rate in humans. Therefore, a precise risk assessment on the basis of updated data is very important. This article gives an overview about the current CCHFV prevalence in cattle in Mauritania. METHODS AND FINDINGS: A seroprevalence study was carried out using 495 cattle sera from Mauritania, which were collected in the year 2013. The sera were analyzed by an inhouse CCHFV-IgG-ELISA. As second screening test, an adapted commercial CCHFV-IgG-ELISA was performed. Inconclusive sera were additionally tested by a modified commercial CCHFV-IgG-IFA. All assays showed high diagnostic sensitivity (>95%) and specificity (>98%). The overall prevalence of CCHFV-specific antibodies found in Mauritanian cattle was 67%, ranging from 56% to 90% in different provinces. CONCLUSION: This study shows a very high CCHFV-specific antibody prevalence in cattle in Mauritania. It is the highest seroprevalence detected in Mauritania so far. This strengthens the hypothesis that CCHFV is a serious and ongoing threat for public health in Mauritania.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Doenças dos Bovinos/virologia
Vírus da Febre Hemorrágica da Crimeia-Congo
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/sangue
Doenças dos Bovinos/epidemiologia
Imunofluorescência
Imunoglobulina G/sangue
Mauritânia/epidemiologia
Estudos Soroepidemiológicos
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Immunoglobulin G)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE
[do] DOI:10.1089/vbz.2016.2084


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[PMID]:28381273
[Au] Autor:Ould Ahmedou Salem MS; Mint Lekweiry K; Bouchiba H; Pascual A; Pradines B; Ould Mohamed Salem Boukhary A; Briolant S; Basco LK; Bogreau H
[Ad] Endereço:Unité de Recherche «Génomes et Milieux¼, Faculté des Sciences et Techniques, Université de Nouakchott Al-Aasriya, Nouveau campus universitaire, BP 5026, Nouakchott, Mauritania. salem0606@yahoo.fr.
[Ti] Título:Characterization of Plasmodium falciparum genes associated with drug resistance in Hodh Elgharbi, a malaria hotspot near Malian-Mauritanian border.
[So] Source:Malar J;16(1):140, 2017 Apr 05.
[Is] ISSN:1475-2875
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: A malaria hotspot in the southeastern region of Mauritania, near the Malian border, may hamper malaria control strategies. The objectives were to estimate the prevalence of genetic polymorphisms associated with drug resistance in Plasmodium falciparum isolates and establish baseline data. METHODS: The study was conducted in two malaria-endemic areas in Hodh Elgharbi, situated in the Malian-Mauritanian border area. Blood samples were collected from symptomatic patients. Single nucleotide polymorphisms in Pfcrt, Pfmdr1, Pfdhfr, and Pfdhps were genotyped using PCR-restriction fragment length polymorphism, DNA sequencing and primer extension. The Pfmdr1 gene copy number was determined by real-time PCR. RESULTS: Of 280 P. falciparum-infected patients, 193 (68.9%) carried the Pfcrt 76T mutant allele. The Pfmdr1 86Y and 184F mutations were found in 61 (23.1%) of 264 isolates and 167 (67.6%) of 247 samples that were successfully genotyped, respectively. Pfmdr1 mutant alleles 1034C, 1042D and 1246Y were rarely observed. Of 102 P. falciparum isolates analysed, ten (9.8%) had more than one copy of Pfmdr1 gene. The prevalence of isolates harbouring at least triple mutant Pfdhfr 51I, 59R, 108 N/T was 42% (112/268), of which 42 (37.5%) had an additional Pfdhps 437G mutation. The Pfdhps 540E mutation was observed in four isolates (1.5%), including three associated with Pfdhfr triple mutant. Only two quintuple mutants (Pfdhfr-51I-59R-108N Pfdhps-437G-540E) were observed. CONCLUSIONS: The observed mutations in Pfdhfr, Pfdhps, Pfmdr1, and Pfcrt may jeopardize the future of seasonal malaria chemoprevention based on amodiaquine-sulfadoxine-pyrimethamine, intermittent preventive treatment for pregnant women using sulfadoxine-pyrimethamine, and treatment with artesunate-amodiaquine. Complementary studies should be carried out to document the distribution, origin and circulation of P. falciparum populations in this region and more widely in the country to assess the risk of the spread of resistance.
[Mh] Termos MeSH primário: Antimaláricos/farmacologia
Resistência a Medicamentos
Genes de Protozoários
Malária Falciparum/parasitologia
Plasmodium falciparum/efeitos dos fármacos
Plasmodium falciparum/genética
Polimorfismo de Nucleotídeo Único
[Mh] Termos MeSH secundário: Doenças Assintomáticas
DNA de Protozoário/química
DNA de Protozoário/genética
Dosagem de Genes
Seres Humanos
Mali
Mauritânia
Plasmodium falciparum/isolamento & purificação
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Prevalência
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimalarials); 0 (DNA, Protozoan)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1186/s12936-017-1791-2


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[PMID]:28035638
[Au] Autor:Ba H; Ahouidi AD; Duffy CW; Deh YB; Diedhiou C; Tandia A; Diallo MY; Assefa S; Lô BB; Elkory MB; Conway DJ
[Ad] Endereço:Institut national de recherche en santé publique (INRSP), BP 695, Nouakchott, Mauritanie. hampateba2001@yahoo.fr.
[Ti] Título:[Evaluation of malaria rapid diagnostic test Optimal-IT® pLDH along the Plasmodium falciparum distribution limit in Mauritania].
[Ti] Título:Evaluation du test de diagnostic rapide du paludisme OptiMal-IT® pLDH à la limite de la distribution de Plasmodium falciparum en Mauritanie..
[So] Source:Bull Soc Pathol Exot;110(1):31-37, 2017 Feb.
[Is] ISSN:0037-9085
[Cp] País de publicação:France
[La] Idioma:fre
[Ab] Resumo:Performance of the malaria Rapid Diagnostic Test (RDT) OptiMal-IT® was evaluated in Mauritania where malaria is low and dependent on a short transmission season. Slide microscopy was considered as the reference method of diagnosis. Febrile patients with suspected malaria were recruited from six health facilities, 3 urban and 3 rural, during two periods (December 2011 to February 2012, and August 2012 to March 2013). Overall, 780 patients were sampled, with RDT and thick blood film microscopy results being obtained for 759 of them. Out of 774 slides examined, of which 200 were positive, P. falciparum and P. vivax mono-infections were detected in 63.5% (127) and 29.5% (59), while P. falciparum/P. vivax coinfections were detected in 7% (14). Both species were observed in all study sites, although in significantly different proportions. The proportions of thick blood film and OptiMal-IT® RDT positive individuals was 26.3% and 30.3% respectively. Sensitivity and specificity of OptiMal-IT® RDT were 89% [95% CI, 84.7-93.3] and 91.1% [88.6-93.4]. Positives and negative predictive values were 78.1% [72.2-83.7] and 95.9% [94.1-97.5]. These diagnostic values are similar to those generally reported elsewhere, and support the use of RDTs as the main diagnostic tool for malaria in Mauritanian health facilities. In the future, choice of RDTs to be used must take account of thermostability in a hot, dry environment and their ability to detect P. falciparum and P. vivax.
[Mh] Termos MeSH primário: Testes Diagnósticos de Rotina/métodos
Malária Falciparum/diagnóstico
[Mh] Termos MeSH secundário: Antígenos de Protozoários/sangue
Seres Humanos
Malária Falciparum/sangue
Malária Falciparum/epidemiologia
Mauritânia/epidemiologia
Plasmodium falciparum/imunologia
Valor Preditivo dos Testes
Kit de Reagentes para Diagnóstico
Sensibilidade e Especificidade
Fatores de Tempo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Protozoan); 0 (Reagent Kits, Diagnostic)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161231
[St] Status:MEDLINE
[do] DOI:10.1007/s13149-017-0541-y


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[PMID]:28029091
[Au] Autor:Rissmann M; Eiden M; El Mamy BO; Isselmou K; Doumbia B; Ziegler U; Homeier-Bachmann T; Yahya B; Groschup MH
[Ad] Endereço:Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut,Greifswald - Insel Riems,Germany.
[Ti] Título:Serological and genomic evidence of Rift Valley fever virus during inter-epidemic periods in Mauritania.
[So] Source:Epidemiol Infect;145(5):1058-1068, 2017 04.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Rift Valley fever virus (RVFV) is an emerging pathogen of major concern throughout Africa and the Arabian Peninsula, affecting both livestock and humans. In the past recurrent epidemics were reported in Mauritania and studies focused on the analysis of samples from affected populations during acute outbreaks. To verify characteristics and presence of RVFV during non-epidemic periods we implemented a multi-stage serological and molecular analysis. Serum samples of small ruminants, cattle and camels were obtained from Mauritania during an inter-epidemic period in 2012-2013. This paper presents a comparative analysis of potential variations and shifts of antibody presence and the capability of inter-epidemic infections in Mauritanian livestock. We observed distinct serological differences between tested species (seroprevalence: small ruminants 3·8%, cattle 15·4%, camels 32·0%). In one single bovine from Nouakchott, a recent RVF infection could be identified by the simultaneous detection of IgM antibodies and viral RNA. This study indicates the occurrence of a low-level enzootic RVFV circulation in livestock in Mauritania. Moreover, results indicate that small ruminants can preferably act as sentinels for RVF surveillance.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Epidemias
RNA Viral/sangue
Febre do Vale de Rift/epidemiologia
Vírus da Febre do Vale do Rift/isolamento & purificação
Ruminantes
[Mh] Termos MeSH secundário: Animais
Mauritânia/epidemiologia
Febre do Vale de Rift/imunologia
Febre do Vale de Rift/virologia
Vírus da Febre do Vale do Rift/genética
Vírus da Febre do Vale do Rift/imunologia
Estudos Soroepidemiológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (RNA, Viral)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161229
[St] Status:MEDLINE
[do] DOI:10.1017/S0950268816003022


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[PMID]:28017635
[Au] Autor:Boué F; El Berbri I; Hormaz V; Boucher JM; El Mamy AB; Traore A; Fihri OF; Petavy AF; Dakkak A; Umhang G
[Ad] Endereço:ANSES, Nancy Laboratory for Rabies and Wildlife, National Reference Laboratory for Echinococcus spp., Wildlife Surveillance and Eco-epidemiology Unit, Technopôle Agricole et Vétérinaire, CS 40009, 54220 Malzéville, France. Electronic address: franck.boue@anses.fr.
[Ti] Título:Use of FTA card methodology for sampling and molecular characterization of Echinococcus granulosus sensu lato in Africa.
[So] Source:Exp Parasitol;173:29-33, 2017 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cystic Echinococcosis is a parasitic disease caused by the cestode Echinococcus granulosus widely distributed in Africa. Monitoring of this parasite requires access to cyst samples on intermediate hosts observed at the slaughterhouse. In order to facilitate sampling in the field and analysis, the French National Reference Laboratory for Echinococcus spp. has developed a tissue derived from DNA sampling with FTA card technology. The DNA samples were taken by applying the FTA paper on the germinal layer after opening the cysts. The sampling technique was validated using frozen cysts (n = 76) stored in the laboratory and from field samples (n = 134) taken at the slaughterhouse by veterinarian technicians during meat inspection in Morocco, Mali and Mauritania. DNA was extracted after several weeks of storage at room temperature. PCR assays were performed using primers for generic cestode (cox1) and amplified fragments were sequenced. All samples taken in the lab and 80% of field samples were capable of molecular characterization. Cyst-derived DNA from FTA samples can be useful for easy sampling, storage and rapid, safe and cheap shipment. The use of the FTA methodology will facilitate studies in the field to investigate the presence and genetic characterization of E. granulosus sensu lato in African countries.
[Mh] Termos MeSH primário: DNA de Helmintos/análise
Equinococose/veterinária
Echinococcus granulosus/classificação
Echinococcus granulosus/genética
Parasitologia/métodos
Manejo de Espécimes/métodos
[Mh] Termos MeSH secundário: Matadouros
Animais
Animais Domésticos/parasitologia
Animais Selvagens/parasitologia
DNA de Helmintos/isolamento & purificação
Equinococose/diagnóstico
Equinococose/parasitologia
Echinococcus granulosus/isolamento & purificação
Mali
Mauritânia
Marrocos
Reação em Cadeia da Polimerase
Manejo de Espécimes/instrumentação
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Helminth)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161227
[St] Status:MEDLINE


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[PMID]:27935801
[Au] Autor:Philibert A; Ravit M; Ridde V; Dossa I; Bonnet E; Bedecarrats F; Dumont A
[Ad] Endereço:Interdisciplinary Research Centre on Well-being, Health, Society and Environment (Cinbiose), University of Quebec in Montreal, Montreal, Québec, Canada.
[Ti] Título:Maternal and neonatal health impact of obstetrical risk insurance scheme in Mauritania: a quasi experimental before-and-after study.
[So] Source:Health Policy Plan;32(3):405-417, 2017 Apr 01.
[Is] ISSN:1460-2237
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A variety of health financing schemes shaped on pre-payment scheme have been implemented across Sub-Saharan Africa (SSA) to address the Millennium Development Goals (MDGs). In Mauritania, the Obstetric Risk Insurance package (ORI) focusing on maternal and perinatal health has been progressively implemented at the health district level since 2002. Here, our main objective was to assess the effectiveness of the ORI in increasing facility-based delivery rates, as well as increases in family planning, antenatal and postnatal care, caesarean delivery and neonatal health, from demographic and health survey data between 2002 and 2011. We also examined whether the effects of the ORI varied between strata of the population. The study was based on a quasi-experimental before-and-after design to assess the causal link between availability of ORI and increase in use of maternal health services and neonatal mortality. In combination with geographical information system, difference-in-differences and odd ratio approaches were used to address our objectives. Indicators of access to care for pregnant women and neonatal health and improved in both non-intervention and intervention groups during the study period. There was no global effect of the availability of ORI on facility-based delivery rates, nor on the use of antenatal and postnatal care services, except for qualified antenatal services. However, delivery rates in local health centres with ORI increased more rapidly than in those with no ORI, the contrary was shown for hospitals. Caesarean delivery and family planning decreased with ORI. Although late neonatal mortality rates remained low in the country, a significant decrease was seen in districts without ORI. Except for some strata of the population, ORI has not really met its objective of attracting more pregnant women towards facility-based health care.
[Mh] Termos MeSH primário: Instalações de Saúde/utilização
Mortalidade Infantil
Seguro Saúde/economia
Serviços de Saúde Materna/utilização
[Mh] Termos MeSH secundário: Adolescente
Adulto
Serviços de Planejamento Familiar
Feminino
Inquéritos Epidemiológicos
Seres Humanos
Lactente
Mortalidade Materna
Mauritânia
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:H
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1093/heapol/czw142



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