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[PMID]:28465095
[Au] Autor:Arriola CS; Vasconez N; Thompson MG; Olsen SJ; Moen AC; Bresee J; Ropero AM
[Ad] Endereço:Epidemic Intelligence Service Program, Centers for Disease Control and Prevention, Atlanta, GA, USA; Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: wus3@cdc.gov.
[Ti] Título:Association of influenza vaccination during pregnancy with birth outcomes in Nicaragua.
[So] Source:Vaccine;35(23):3056-3063, 2017 05 25.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Studies have shown that influenza vaccination during pregnancy reduces the risk of influenza disease in pregnant women and their offspring. Some have proposed that maternal vaccination may also have beneficial effects on birth outcomes. In 2014, we conducted an observational study to test this hypothesis using data from two large hospitals in Managua, Nicaragua. METHODS: We conducted a retrospective cohort study to evaluate associations between influenza vaccination and birth outcomes. We carried out interviews and reviewed medical records post-partum to collect data on demographics, influenza vaccination during pregnancy, birth outcomes and other risk factors associated with adverse neonatal outcomes. We used influenza surveillance data to adjust for timing of influenza circulation. We assessed self-reports of influenza vaccination status by further reviewing medical records of those who self-reported but did not have readily available evidence of vaccination status. We performed multiple logistic regression (MLR) and propensity score matching (PSM). RESULTS: A total of 3268 women were included in the final analysis. Of these, 55% had received influenza vaccination in 2014. Overall, we did not observe statistically significant associations between influenza vaccination and birth outcomes after adjusting for risk factors, with either MLR or PSM. With PSM, after adjusting for risk factors, we observed protective associations between influenza vaccination in the second and third trimester and preterm birth (aOR: 0.87; 95% confidence interval (CI): 0.75-0.99 and aOR: 0.66; 95% CI: 0.45-0.96, respectively) and between influenza vaccination in the second trimester and low birth weight (aOR: 0.80; 95% CI: 0.64-0.97). CONCLUSIONS: We found evidence to support an association between influenza vaccination and birth outcomes by trimester of receipt with data from an urban population in Nicaragua. The study had significant selection and recall biases. Prospective studies are needed to minimize these biases.
[Mh] Termos MeSH primário: Vacinas contra Influenza/administração & dosagem
Influenza Humana/prevenção & controle
Complicações Infecciosas na Gravidez/prevenção & controle
Resultado da Gravidez
[Mh] Termos MeSH secundário: Adolescente
Adulto
Estudos de Coortes
Feminino
Seres Humanos
Recém-Nascido de Baixo Peso
Recém-Nascido
Modelos Logísticos
Registros Médicos
Nicarágua/epidemiologia
Gravidez
Complicações Infecciosas na Gravidez/epidemiologia
Trimestres da Gravidez
Nascimento Prematuro/epidemiologia
Pontuação de Propensão
Estudos Retrospectivos
População Urbana/estatística & dados numéricos
Vacinação
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Influenza Vaccines)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


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[PMID]:27778409
[Au] Autor:Winston ME; Kronauer DJ; Moreau CS
[Ad] Endereço:Committee on Evolutionary Biology, University of Chicago, Chicago, IL, 60637, USA.
[Ti] Título:Early and dynamic colonization of Central America drives speciation in Neotropical army ants.
[So] Source:Mol Ecol;26(3):859-870, 2017 Feb.
[Is] ISSN:1365-294X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The emergence of the Isthmus of Panama is one of the most important events in recent geological history, yet its timing and role in fundamental evolutionary processes remain controversial. While the formation of the isthmus was complete around 3 million years ago (Ma), recent studies have suggested prior intercontinental biotic exchange. In particular, the possibility of early intermittent land bridges facilitating colonization constitutes a potential mechanism for speciation and colonization before full closure of the isthmus. To test this hypothesis, we employed genomic methods to study the biogeography of the army ant genus Eciton, a group of keystone arthropod predators in Neotropical rainforests. Army ant colonies are unable to disperse across water and are therefore ideally suited to study the biogeographic impact of land bridge formation. Using a reduced representation genome sequencing approach, we show that all strictly Central American lineages of Eciton diverged from their respective South American sister lineage between 4 and 7 Ma, significantly prior to the complete closure of the isthmus. Furthermore, three of the lineage pairs form extensive and coincident secondary contact zones in Costa Rica and Nicaragua, with no evidence of gene flow. Such a discrete and repeated biogeographic pattern indicates at least two waves of army ant dispersal into Central America that were separated by significant genetic divergence times. Thus, by integrating phylogenomic, population genomic and geographic evidence, we show that early colonization of Central America across the emerging Isthmus of Panamá drove parallel speciation in Eciton army ants.
[Mh] Termos MeSH primário: Formigas/classificação
Especiação Genética
[Mh] Termos MeSH secundário: Animais
Costa Rica
Feminino
Nicarágua
Panamá
Filogenia
Filogeografia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1111/mec.13846


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[PMID]:29031287
[Au] Autor:Soto LA; Caballero N; Fuentes LR; Muñoz PT; Gómez Echevarría JR; López MP; Bornay Llinares FJ; Stanford JL; Stanford CA; Donoghue HD
[Ad] Endereço:Universidad Miguel Hernández de Elche, Alicante, Spain.
[Ti] Título:Leprosy Associated with Atypical Cutaneous Leishmaniasis in Nicaragua and Honduras.
[So] Source:Am J Trop Med Hyg;97(4):1103-1110, 2017 Oct.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Central America, few cases of leprosy have been reported, but the disease may be unrecognized. Diagnosis is based on clinical criteria and histology. Preliminary field work in Nicaragua and Honduras found patients, including many children, with skin lesions clinically suggestive of atypical cutaneous leishmaniasis or indeterminate leprosy. Histology could not distinguish these diseases although acid-fast organisms were visible in a few biopsies. Lesions healed after standard antimicrobial therapy for leprosy. In the present study, patients, family members, and other community members were skin-tested and provided nasal swabs and blood samples. Biopsies were taken from a subgroup of patients with clinical signs of infection. Two laboratories analyzed samples, using local in-house techniques. , spp. and were detected using polymerase chain reactions. DNA was detected in blood samples and nasal swabs, including some cases where leprosy was not clinically suspected. spp. were also detected in blood and nasal swabs. Most biopsies contained DNA and coinfection of spp. with occurred in 33% of cases. DNA was also detected and sequenced from Nicaraguan and Honduran environmental samples. In conclusion, leprosy and leishmaniasis are present in both regions, and leprosy appears to be widespread. The nature of any relationship between these two pathogens and the epidemiology of these infections need to be elucidated.
[Mh] Termos MeSH primário: Leishmania/isolamento & purificação
Leishmaniose Cutânea/diagnóstico
Leishmaniose Cutânea/epidemiologia
Hanseníase/diagnóstico
Hanseníase/epidemiologia
Mycobacterium leprae/isolamento & purificação
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Honduras/epidemiologia
Seres Humanos
Masculino
Meia-Idade
Nicarágua/epidemiologia
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0622


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[PMID]:28863466
[Au] Autor:Waggoner JJ; Gresh L; Mohamed-Hadley A; Balmaseda A; Soda KJ; Abeynayake J; Sahoo MK; Liu Y; Kuan G; Harris E; Pinsky BA
[Ad] Endereço:Department of Medicine, Division of Infectious Diseases and Geographic Medicine.
[Ti] Título:Characterization of Dengue Virus Infections Among Febrile Children Clinically Diagnosed With a Non-Dengue Illness, Managua, Nicaragua.
[So] Source:J Infect Dis;215(12):1816-1823, 2017 Jun 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: We sought to characterize dengue virus (DENV) infections among febrile children enrolled in a pediatric cohort study who were clinically diagnosed with a non-dengue illness ("C cases"). Methods: DENV infections were detected and viral load quantitated by real-time reverse transcription-polymerase chain reaction in C cases presenting between January 2007 and January 2013. Results: One hundred forty-one of 2892 C cases (4.88%) tested positive for DENV. Of all febrile cases in the study, DENV-positive C cases accounted for an estimated 52.0% of patients with DENV viremia at presentation. Compared with previously detected, symptomatic dengue cases, DENV-positive C cases were significantly less likely to develop long-lasting humoral immune responses to DENV, as measured in healthy annual serum samples (79.7% vs 47.8%; P < .001). Humoral immunity was associated with viral load at presentation: 40 of 43 patients (93.0%) with a viral load ≥7.0 log10 copies/mL serum developed the expected rise in anti-DENV antibodies in annual samples versus 13 of 68 (19.1%) patients with a viral load below this level (P < .001). Conclusions: Antibody responses to DENV-positive C cases differ from responses to classic symptomatic dengue. These findings have important implications for DENV transmission modeling, immunology, and epidemiologic surveillance.
[Mh] Termos MeSH primário: Formação de Anticorpos/imunologia
Vírus da Dengue/imunologia
Dengue/diagnóstico
[Mh] Termos MeSH secundário: Anticorpos Antivirais/sangue
Estudos de Casos e Controles
Criança
Pré-Escolar
Estudos de Coortes
Dengue/epidemiologia
Dengue/virologia
Ensaio de Imunoadsorção Enzimática
Feminino
Febre/etiologia
Seres Humanos
Incidência
Masculino
Nicarágua/epidemiologia
RNA Viral/sangue
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Viremia/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (RNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170903
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix195


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[PMID]:28722618
[Au] Autor:Fischer RSB; Mandayam S; Chavarria D; Vangala C; Nolan MS; Garcia LL; Palma L; Garcia F; García-Trabanino R; Murray KO
[Ad] Endereço:Section of Tropical Medicine, Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine and Texas Children's Hospital, Houston, Texas.
[Ti] Título:Clinical Evidence of Acute Mesoamerican Nephropathy.
[So] Source:Am J Trop Med Hyg;97(4):1247-1256, 2017 Oct.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mesoamerican nephropathy (MeN), an epidemic of unexplained kidney disease in Central America, affects mostly young, healthy individuals. Its etiology is a mystery that requires urgent investigation. Largely described as a chronic kidney disease (CKD), no acute clinical scenario has been characterized. An understanding of the early disease process could elucidate an etiology and guide treatment and prevention efforts. We sought to document the earliest clinical signs in patients with suspected MeN in a high-risk population in Nicaragua. Physicians at a local hospital identified suspect cases and documented clinical/laboratory data, demographics, and medical histories. Over a 1-year period, physicians identified 255 mostly young (median 29 years), male (89.5%) patients with elevated creatinine or reduced creatinine clearance. Mean serum creatinine (2.0 ± 0.6 mg/dL) revealed a 2-fold increase from baseline, and half had stage 2 or 3 acute kidney injury. Leukocyturia (98.4%), leukocytosis (81.4%), and neutrophilia (86.2%) predominated. Nausea (59.4%), back pain (57.9%), fever (54.6%), vomiting (50.4%), headache (47.3%), and muscle weakness (45.0%) were common. A typical case of acute MeN presented with elevated (or increased ≥ 0.3 mg/dL or ≥ 1.5-fold from baseline) creatinine, no hypertension or diabetes, leukocyturia, and at least two of fever, nausea or vomiting, back pain, muscle weakness, headache, or leukocytosis and/or neutrophilia. Rapid progression (median 90 days) to CKD was recorded in 8.5% of patients. This evidence can serve as the basis of a sensitive and urgently needed case definition for disease surveillance of early-stage, acute MeN.
[Mh] Termos MeSH primário: Lesão Renal Aguda/epidemiologia
Lesão Renal Aguda/patologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Feminino
Seres Humanos
Inflamação/patologia
Masculino
Meia-Idade
Nicarágua/epidemiologia
População Rural
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170720
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.17-0260


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[PMID]:28722577
[Au] Autor:Gruber JF; Bowman NM; Becker-Dreps S; Reyes Y; Belson C; Michaels KC; Bucardo F
[Ad] Endereço:Department of Epidemiology, University of North Carolina at Chapel Hill Gillings School of Global Public Health, Chapel Hill, North Carolina.
[Ti] Título:Risk Factors for Norovirus Gastroenteritis among Nicaraguan Children.
[So] Source:Am J Trop Med Hyg;97(3):937-943, 2017 Sep.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Norovirus is a leading cause of pediatric gastroenteritis. Understanding norovirus epidemiology is essential for reducing disease burden. We conducted a case-control study to describe the distribution, clinical features, and risk factors of norovirus gastroenteritis among children < 5 years of age in León, Nicaragua. Cases were children testing positive for norovirus and controls were children living in the cases' communities. Study staff interviewed mothers of enrolled cases and controls to obtain detailed exposure information including food, water, and sanitation sources; recent exposures; household characteristics; and handwashing practices. In addition, study staff requested stool samples to be tested for norovirus from select household members. We used descriptive statistics to understand the epidemiologic and clinical features of gastroenteritis episodes. To analyze potential risk factors, we used Firth's penalized logistic regression to estimate crude and adjusted odds ratios (ORs) and corresponding 95% confidence intervals (CIs). There were 102 children with gastroenteritis, 18 cases of norovirus and 31 controls. Norovirus cases occurred later in the year, corresponding to a delay in the rainy season. Cases were more likely to have a household member with norovirus in their stool as compared with controls [crude OR: 13.3 (95% CI: 2.5, 136.2) and adjusted OR: 11.5 (95% CI: 1.6, 223.2)]. In addition, alcohol-based hand sanitizer use among household members was reported for 10 (32%) of controls and but never for cases. Further research is needed to understand household transmission of norovirus in low- and middle-income countries and the potential impact of hand sanitizer use.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/virologia
Gastroenterite/epidemiologia
Gastroenterite/virologia
Norovirus
[Mh] Termos MeSH secundário: Infecções por Caliciviridae/epidemiologia
Estudos de Casos e Controles
Pré-Escolar
Feminino
Seres Humanos
Lactente
Masculino
Nicarágua/epidemiologia
Fatores de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170720
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0799


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[PMID]:28542624
[Au] Autor:Nordenstedt N; Marcenaro D; Chilagane D; Mwaipopo B; Rajamäki ML; Nchimbi-Msolla S; Njau PJR; Mbanzibwa DR; Valkonen JPT
[Ad] Endereço:Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland.
[Ti] Título:Pathogenic seedborne viruses are rare but Phaseolus vulgaris endornaviruses are common in bean varieties grown in Nicaragua and Tanzania.
[So] Source:PLoS One;12(5):e0178242, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Common bean (Phaseolus vulgaris) is an annual grain legume that was domesticated in Mesoamerica (Central America) and the Andes. It is currently grown widely also on other continents including Africa. We surveyed seedborne viruses in new common bean varieties introduced to Nicaragua (Central America) and in landraces and improved varieties grown in Tanzania (eastern Africa). Bean seeds, harvested from Nicaragua and Tanzania, were grown in insect-controlled greenhouse or screenhouse, respectively, to obtain leaf material for virus testing. Equal amounts of total RNA from different samples were pooled (30-36 samples per pool), and small RNAs were deep-sequenced (Illumina). Assembly of the reads (21-24 nt) to contiguous sequences and searches for homologous viral sequences in databases revealed Phaseolus vulgaris endornavirus 1 (PvEV-1) and PvEV-2 in the bean varieties in Nicaragua and Tanzania. These viruses are not known to cause symptoms in common bean and are considered non-pathogenic. The small-RNA reads from each pool of samples were mapped to the previously characterized complete PvEV-1 and PvEV-2 sequences (genome lengths ca. 14 kb and 15 kb, respectively). Coverage of the viral genomes was 87.9-99.9%, depending on the pool. Coverage per nucleotide ranged from 5 to 471, confirming virus identification. PvEV-1 and PvEV-2 are known to occur in Phaseolus spp. in Central America, but there is little previous information about their occurrence in Nicaragua, and no information about occurrence in Africa. Aside from Cowpea mild mosaic virus detected in bean plants grown from been seeds harvested from one region in Tanzania, no other pathogenic seedborne viruses were detected. The low incidence of infections caused by pathogenic viruses transmitted via bean seeds may be attributable to new, virus-resistant CB varieties released by breeding programs in Nicaragua and Tanzania.
[Mh] Termos MeSH primário: Phaseolus/virologia
Vírus de Plantas/fisiologia
Sementes/virologia
[Mh] Termos MeSH secundário: Comovirus/genética
Nicarágua
Vírus de Plantas/genética
RNA Viral/genética
Tanzânia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178242


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[PMID]:28395541
[Au] Autor:Pernudy-Ubau A; Salinas-Molina J; Requenez Y; Ortiz-Lopez M; Puller AC; García-Rosales K; Rodríguez-Estrada A; Rodríguez-Romero W; Mejía-Baltodano G; Luo HY; Chui DHK
[Ad] Endereço:a Molecular Biology Laboratory 'M.A. Elmer Cisneros in Memoriam ', Polytechnic Institute of Health, National Autonomous University of Nicaragua (UNAN) , Managua , Nicaragua.
[Ti] Título:Hb Presbyterian (HBB: c.327C>G) in a Nicaraguan Family.
[So] Source:Hemoglobin;41(1):50-52, 2017 Jan.
[Is] ISSN:1532-432X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hemoglobin (Hb) is the protein responsible for oxygen transportation. It is a tetrameric protein comprising two α- and two ß-globin subunits. In the literature, a large number of mutations in the α- and ß-globin genes have been documented. Among these mutations, Hb Presbyterian (HBB: c.327 C>G), is a naturally occurring mutant exerting low oxygen affinity. The C to G exchange (AAC>AAG) at codon 108 of the ß-globin gene results in the substitution of asparagine by lysine. Here, we document the identification of HBB: c.327 C>G in a 6-year-old female patient and her father from Nicaragua and Cuba, respectively. The presence of the abnormal Hb was confirmed by cellulose acetate electrophoresis, high performance liquid chromatography (HPLC) and genomic DNA sequencing. The ß-globin gene sequences for both, father and daughter, disclosed the heterozygous mutation at codon 108 to be Hb Presbyterian or HBB: c.327 C>G. The mutant Hb was previously reported in four families from North America, Germany, Japan and Spain, respectively. This is the fifth family carrying HBB: c.327 C>G described to date and the first report from Latin America.
[Mh] Termos MeSH primário: Hemoglobinopatias/diagnóstico
Hemoglobinopatias/genética
Hemoglobinas Anormais/genética
Mutação
Globinas beta/genética
[Mh] Termos MeSH secundário: Adulto
Alelos
Criança
Códon
Análise Mutacional de DNA
Feminino
Genótipo
Hemoglobinopatias/sangue
Seres Humanos
Masculino
Nicarágua
Fenótipo
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon); 0 (Hemoglobins, Abnormal); 0 (beta-Globins); 69227-31-2 (hemoglobin Presbyterian)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.1080/03630269.2017.1302949


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[PMID]:28381638
[Au] Autor:Hertz T; Beatty PR; MacMillen Z; Killingbeck SS; Wang C; Harris E
[Ad] Endereço:Department of Microbiology, Immunology and Genetics, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
[Ti] Título:Antibody Epitopes Identified in Critical Regions of Dengue Virus Nonstructural 1 Protein in Mouse Vaccination and Natural Human Infections.
[So] Source:J Immunol;198(10):4025-4035, 2017 May 15.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dengue is a global public health problem and is caused by four dengue virus (DENV) serotypes (DENV1-4). A major challenge in dengue vaccine development is that cross-reactive anti-DENV Abs can be protective or potentially increase disease via Ab-dependent enhancement. DENV nonstructural protein 1 (NS1) has long been considered a vaccine candidate as it avoids Ab-dependent enhancement. In this study, we evaluated survival to challenge in a lethal DENV vascular leak model in mice immunized with NS1 combined with aluminum and magnesium hydroxide, monophosphoryl lipid A + AddaVax, or Sigma adjuvant system+CpG DNA, compared with mice infected with a sublethal dose of DENV2 and mice immunized with OVA (negative control). We characterized Ab responses to DENV1, 2, and 3 NS1 using an Ag microarray tiled with 20-mer peptides overlapping by 15 aa and identified five regions of DENV NS1 with significant levels of Ab reactivity in the NS1 + monophosphoryl lipid A + AddaVax group. Additionally, we profiled the Ab responses to NS1 of humans naturally infected with DENV2 or DENV3 in serum samples from Nicaragua collected at acute, convalescent, and 12-mo timepoints. One region in the wing domain of NS1 was immunodominant in both mouse vaccination and human infection studies, and two regions were identified only in NS1-immunized mice; thus, vaccination can generate Abs to regions that are not targeted in natural infection and could provide additional protection against lethal DENV infection. Overall, we identified a small number of immunodominant regions, which were in functionally important locations on the DENV NS1 protein and are potential correlates of protection.
[Mh] Termos MeSH primário: Antígenos Virais/imunologia
Vacinas contra Dengue/imunologia
Vírus da Dengue/imunologia
Dengue/imunologia
Epitopos/imunologia
Proteínas não Estruturais Virais/imunologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos
Adolescente
Animais
Anticorpos Antivirais/sangue
Criança
Pré-Escolar
Reações Cruzadas
Dengue/epidemiologia
Dengue/virologia
Vírus da Dengue/química
Modelos Animais de Doenças
Epitopos/química
Epitopos/genética
Epitopos/isolamento & purificação
Feminino
Seres Humanos
Imunidade Inata
Epitopos Imunodominantes/genética
Lactente
Masculino
Camundongos
Nicarágua/epidemiologia
Estudos Prospectivos
Sorotipagem
Vacinação
Proteínas não Estruturais Virais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (Dengue Vaccines); 0 (Epitopes); 0 (Immunodominant Epitopes); 0 (NS1 protein, Dengue virus type 2); 0 (NS1 protein, Dengue virus type 3); 0 (NS1 protein, dengue-1 virus); 0 (Viral Nonstructural Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1700029


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[PMID]:28330443
[Au] Autor:Thompson MG; Hunt DR; Arbaji AK; Simaku A; Tallo VL; Biggs HM; Kulb C; Gordon A; Khader IA; Bino S; Lucero MG; Azziz-Baumgartner E; Shifflett P; Sanchez F; Marar BI; Bakalli I; Simões EA; Levine MZ; Meece JK; Balmaseda A; Al-Sanouri TM; Dhimolea M; de Jesus JN; Thornburg NJ; Gerber SI; Gresh L; IRIS Network
[Ad] Endereço:Influenza Division, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA. Isq8@cdc.gov.
[Ti] Título:Influenza and respiratory syncytial virus in infants study (IRIS) of hospitalized and non-ill infants aged <1 year in four countries: study design and methods.
[So] Source:BMC Infect Dis;17(1):222, 2017 Mar 22.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: This multi-country prospective study of infants aged <1 year aims to assess the frequency of influenza virus and respiratory syncytial virus (RSV) infections associated with hospitalizations, to describe clinical features and antibody response to infection, and to examine predictors of very severe disease requiring intensive care. METHODS/DESIGN: We are enrolling a hospital-based cohort and a sample of non-ill infants in four countries (Albania, Jordan, Nicaragua, and the Philippines) using a common protocol. We are currently starting year 2 of a 2- to 3-year study and will enroll approximately 3,000 infants hospitalized for any acute illness (respiratory or non-respiratory) during periods of local influenza and/or RSV circulation. After informed consent and within 24 h of admission, we collect blood and respiratory specimens and conduct an interview to assess socio-demographic characteristics, medical history, and symptoms of acute illness (onset ≤10 days). Vital signs, interventions, and medications are documented daily through medical record abstraction. A follow-up health assessment and collection of convalescent blood occurs 3-5 weeks after enrollment. Influenza and RSV infection is confirmed by singleplex real time reverse transcriptase polymerase chain reaction (rRT-PCR) assays. Serologic conversion will be assessed comparing acute and convalescent sera using hemagglutination inhibition assay for influenza antibodies and enzyme-linked immunosorbent assay (ELISA) for RSV. Concurrent with hospital-based enrollment, respiratory specimens are also being collected (and tested by rRT-PCR) from approximately 1,400 non-ill infants aged <1 year during routine medical or preventive care. DISCUSSION: The Influenza and RSV in Infants Study (IRIS) promises to expand our knowledge of the frequency, clinical features, and antibody profiles of serious influenza and RSV disease among infants aged <1 year, quantify the proportion of infections that may be missed by traditional surveillance, and inform decisions about the potential value of existing and new vaccines and other prevention and treatment strategies.
[Mh] Termos MeSH primário: Hospitalização/estatística & dados numéricos
Influenza Humana/epidemiologia
Infecções por Vírus Respiratório Sincicial/epidemiologia
Vírus Sincicial Respiratório Humano/isolamento & purificação
[Mh] Termos MeSH secundário: Albânia/epidemiologia
Anticorpos Antivirais
Feminino
Seres Humanos
Lactente
Influenza Humana/diagnóstico
Jordânia/epidemiologia
Masculino
Nicarágua/epidemiologia
Filipinas/epidemiologia
Prevalência
Estudos Prospectivos
Reação em Cadeia da Polimerase em Tempo Real
Infecções por Vírus Respiratório Sincicial/diagnóstico
Vírus Sinciciais Respiratórios
Fatores de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2299-7



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